CN210204746U - Circulating tumor cell collecting device - Google Patents
Circulating tumor cell collecting device Download PDFInfo
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- CN210204746U CN210204746U CN201920819754.8U CN201920819754U CN210204746U CN 210204746 U CN210204746 U CN 210204746U CN 201920819754 U CN201920819754 U CN 201920819754U CN 210204746 U CN210204746 U CN 210204746U
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Abstract
The utility model relates to a medical treatment appurtenance technical field especially relates to a circulation is collection device for tumor cell, including the surge drum, the bottom of surge drum even has the puncture, and the surge drum is inside to be equipped with a plurality of cavities, and a plurality of cavities include tumor cell elution room, red blood cell collection room and hydrops collection room, and tumor cell elution room and red blood cell collection room are indoor to be equipped with first filtration rete, are equipped with the second filtration rete between red blood cell collection room and the hydrops collection room. By utilizing the collecting device, the tumor cells are eluted, captured and collected on line, and the operation is more convenient.
Description
Technical Field
The utility model relates to the technical field of medical equipment, especially, relate to a circulating tumor cell is with collection device.
Background
Circulating Tumor Cells (CTCs) are a general name of various tumor cells existing in peripheral blood, and fall off from solid tumor focuses (primary focuses and metastatic focuses) due to spontaneous or diagnosis and treatment operations, most CTCs are subjected to apoptosis or phagocytosis after entering the peripheral blood, and a few CTCs can escape and develop into the metastatic focuses, so that the death risk of malignant tumor patients is greatly increased. Circulating tumor cell content is few, lacks the specificity marker moreover, and prior art medical personnel adopt the collection device who has the puncture usually to carry out circulating tumor cell's extraction to the patient, and current collection device simple structure mainly has following problem during the use: when in extraction, some effusion can also enter the collecting device together, thus increasing the separation difficulty of tumor cells; tumor cells collected in the collecting device need to be transferred into other screening devices for subsequent elution, interference components such as red blood cells and the like are washed away, and then the eluted collected matter is detected, so that the existing collecting device cannot capture circulating tumor cells on line.
SUMMERY OF THE UTILITY MODEL
The utility model discloses the technical problem that will solve is: aiming at the defects of the prior art, the collecting device for the circulating tumor cells is reasonable in structural design, can remove accumulated liquid and can elute and capture the tumor cells on line.
In order to solve the technical problem, the technical scheme of the utility model is that:
a collection device for circulating tumor cells comprises a collection cylinder, wherein the bottom of the collection cylinder is connected with a puncture, a plurality of cavities are arranged inside the collection cylinder, the plurality of cavities comprise a tumor cell elution chamber, a red blood cell collection chamber and an effusion collection chamber, a first filter membrane layer is arranged in the tumor cell elution chamber and the red blood cell collection chamber, and a second filter membrane layer is arranged between the red blood cell collection chamber and the effusion collection chamber; the tumor cell elution chamber is positioned at the bottom of the collection cylinder, one side of the tumor cell elution chamber is provided with a tumor cell delivery pipe, and the other side of the tumor cell elution chamber is provided with an eluent conduit; the red blood cell collecting chamber is positioned above the tumor cell eluting chamber, a push rod is arranged in the red blood cell collecting chamber, one end of the push rod is connected with a piston, the other end of the push rod extends out of the red blood cell collecting chamber, and a red blood cell discharging pipe is arranged on one side of the red blood cell collecting chamber; the hydrops collection chamber is located the opposite side of red blood cell collection chamber, one side that the chamber was collected to the hydrops is equipped with hydrops eduction tube.
As an improved technical scheme, air blowing pipes are respectively arranged above and below the eluent guide pipe, and the end parts of the air blowing pipes are connected with extrusion air bags.
As an improved technical scheme, the pore diameter of the first filter membrane layer is 6-10 μm.
As an improved technical scheme, the pore diameter of the second filter membrane layer is 2-4 μm.
As an improved technical scheme, a cleaning solution conduit is further arranged on one side of the red blood cell collecting chamber.
As an improved technical scheme, the bottom of the tumor cell elution chamber is V-shaped.
After the technical scheme is adopted, the beneficial effects of the utility model are that:
the collecting device for circulating tumor cells comprises a collecting barrel, wherein the bottom of the collecting barrel is connected with a puncture, a plurality of cavities are arranged in the collecting barrel, the plurality of cavities comprise a tumor cell eluting chamber, a red blood cell collecting chamber and an effusion collecting chamber, a first filter membrane layer is arranged in the tumor cell eluting chamber and the red blood cell collecting chamber, and a second filter membrane layer is arranged between the red blood cell collecting chamber and the effusion collecting chamber; the tumor cell elution chamber is positioned at the bottom of the collection cylinder, one side of the tumor cell elution chamber is provided with a tumor cell delivery pipe, and the other side of the tumor cell elution chamber is provided with an eluent conduit; the erythrocyte collecting chamber is positioned above the tumor cell eluting chamber, a push rod is arranged in the erythrocyte collecting chamber, one end of the push rod is connected with a piston, the other end of the push rod extends out of the erythrocyte collecting chamber, and an erythrocyte discharging pipe is arranged on one side of the erythrocyte collecting chamber; the hydrops collection chamber is located the opposite side of red blood cell collection chamber, and one side of hydrops collection chamber is equipped with hydrops eduction tube. In practical application, a medical worker firstly inserts a puncture into an affected part to be collected, a worker manually pulls a push rod, effusion and blood carrying tumor cells enter a collecting cylinder along with the puncture, because a first filter membrane layer is arranged between a red blood cell collecting chamber and a tumor cell eluting chamber, the diameter of the red blood cells is 6-8um, and the diameter of the tumor cells is larger than that of the red blood cells, the red blood cells and the effusion can enter the red blood cell collecting chamber through the first filter membrane layer in the pumping process of the push rod, the tumor cells are intercepted in the tumor cell eluting chamber by the first filter membrane layer, the eluent is conveyed into the tumor cell eluting chamber through an eluent conduit, the tumor cells are eluted by the eluent, and finally the tumor cells are led out for detection through the tumor cell conduit; after the components such as the effusion, the red blood cells and the like enter the red blood cell collecting chamber, the effusion can enter the effusion collecting chamber through the second filtering membrane layer, and the push rod is pushed to be finally discharged through the effusion discharge pipe; the components such as the red blood cells are intercepted in the red blood cell collecting chamber and are discharged through the red blood cell calandria; by adopting the collecting device, the on-line elution, capture and collection of the tumor cells are realized, the operation is simple, and the use is more convenient.
Because the upper part and the lower part of the eluent conduit are respectively provided with an air blowing pipe communicated with the tumor cell elution chamber, and the end part of the air blowing pipe is connected with an extrusion air bag. The air bag and the air blowing pipe are squeezed to blow air to the tumor cell eluting chamber, so that the mobility of blood and effusion is improved, and the separation of tumor cells from the effusion, living cells and other components is facilitated.
Since the pore diameter of the first filter membrane layer is 6-10 μm. The first filtering membrane layer with the aperture size can realize the interception of tumor cells and is beneficial to the discharge of effusion and red blood cells.
Since the pore size of the second filter membrane layer is 2-4 μm. The second filtering membrane layer with the aperture size can realize the interception of red blood cells and is beneficial to the drainage of effusion.
Because one side of the red blood cell collecting chamber is also provided with a cleaning solution conduit. Carry the washing liquid to the erythrocyte collection room through the washing liquid pipe in, the washing liquid can pass first filtration rete and second filtration rete respectively, gets into hydrops collection indoor portion and inside tumor cell elution room in proper order, has realized the washing to hydrops collection room, tumor cell elution room and erythrocyte collection room.
Drawings
Fig. 1 is a schematic structural view of a collection device for circulating tumor cells according to the present invention;
the device comprises a collecting cylinder 1, a tumor cell eluting chamber 10, a tumor cell delivery pipe 101, an eluent conduit 102, an air blowing pipe 103, an erythrocyte collecting chamber 11, an erythrocyte discharging pipe 110, a cleaning fluid conduit 111, an effusion collecting chamber 12, an effusion delivery pipe 120, a puncture 2, a first filter membrane layer 3, a second filter membrane layer 4, a push rod 5, a piston 50, a handle 51 and an extrusion air bag 6.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
A collection device for circulating tumor cells is shown in figure 1 and comprises a collection cylinder 1, wherein the bottom of the collection cylinder 1 is connected with a puncture 2, a plurality of cavities are arranged inside the collection cylinder 1 and comprise a tumor cell elution chamber 10, a red blood cell collection chamber 11 and an effusion collection chamber 12, a first filter membrane layer 3 (the aperture of the first filter membrane layer is 6-10 mu m) is arranged in the tumor cell elution chamber 10 and the red blood cell collection chamber 11, a second filter membrane layer 4 (the aperture of the second filter membrane layer is 2-4 mu m) is arranged between the red blood cell collection chamber 11 and the effusion collection chamber 12, a clamping groove is arranged on the inner wall of the collection cylinder, and the first filter membrane layer and the second filter membrane layer are respectively clamped with the inner wall of the collection cylinder; the tumor cell elution chamber 10 is positioned at the bottom of the collection cylinder 1, one side of the tumor cell elution chamber 10 is provided with a tumor cell delivery pipe 101, and the other side of the tumor cell elution chamber 10 is provided with an eluent conduit 101; the erythrocyte collecting chamber 11 is positioned above the tumor cell eluting chamber 10, a push rod 5 is arranged in the erythrocyte collecting chamber 11, one end of the push rod 5 is connected with a piston 50, the other end of the push rod 5 extends out of the erythrocyte collecting chamber 11, the end part is provided with a handle 51, and one side of the erythrocyte collecting chamber 11 is provided with an erythrocyte calandria 110; the effusion collection chamber 12 is located the opposite side of the erythrocyte collection chamber 11, and an effusion outlet tube 120 is arranged on one side of the effusion collection chamber 12.
In practical application, medical staff firstly inserts the puncture into the affected part to be collected, the staff manually pulls the push rod, the effusion and the blood carrying tumor cells enter the collecting cylinder along with the puncture, because the first filter membrane layer is arranged between the red blood cell collecting chamber and the tumor cell eluting chamber, the diameter of the red blood cells is between 6 and 8um, the diameter of the tumor cells is larger than that of the red blood cells, the red blood cells and accumulated liquid can enter a red blood cell collecting chamber through a first filtering membrane layer in the pumping process of the push rod, the tumor cells are trapped in a tumor cell eluting chamber by the first filtering membrane layer, an eluent conduit is connected with a reservoir made of elastic materials and filled with eluent components, the eluent of the reservoir is extruded and conveyed into the tumor cell eluting chamber along the eluent conduit, the tumor cells are eluted by the eluent, and finally the tumor cells are led out through the tumor cell conduit for detection; after the components such as the effusion, the red blood cells and the like enter the red blood cell collecting chamber, the effusion can enter the effusion collecting chamber through the second filtering membrane layer and is finally discharged through the effusion discharge pipe; the components such as the red blood cells are intercepted in the red blood cell collecting chamber and are discharged through the red blood cell calandria; by adopting the collecting device, the on-line elution, capture and collection of the tumor cells are realized, the operation is simple, and the use is more convenient.
Wherein, the upper part and the lower part of the eluent conduit 102 are respectively provided with an air blowing pipe 103 communicated with the tumor cell elution chamber 10, and the end part of the air blowing pipe 103 is connected with an extrusion air bag 6. The air bag and the air blowing pipe are squeezed to blow air to the tumor cell eluting chamber, so that the mobility of blood and effusion is improved, and the separation of tumor cells from the effusion, living cells and other components is facilitated.
Wherein a cleaning solution conduit 111 is further provided at one side of the red blood cell collecting chamber 11. Through the washing liquid storage cylinder of elastic material, with its and washing liquid pipe intercommunication, the extrusion washing liquid storage cylinder carries the washing liquid to the erythrocyte collection indoor, and the washing liquid can pass first filtration rete and second filtration rete respectively, gets into the hydrops in proper order and collects indoor portion and the inside tumor cell elution room, has realized the washing to hydrops collection room, tumor cell elution room and erythrocyte collection room.
The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modifications, equivalents, improvements and the like made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.
Claims (6)
1. A collection device for circulating tumor cells, characterized in that: the device comprises a collecting cylinder, wherein the bottom of the collecting cylinder is connected with a puncture, a plurality of cavities are arranged in the collecting cylinder, the plurality of cavities comprise a tumor cell elution chamber, a red blood cell collection chamber and an effusion collection chamber, a first filter membrane layer is arranged in the tumor cell elution chamber and the red blood cell collection chamber, and a second filter membrane layer is arranged between the red blood cell collection chamber and the effusion collection chamber; the tumor cell elution chamber is positioned at the bottom of the collection cylinder, one side of the tumor cell elution chamber is provided with a tumor cell delivery pipe, and the other side of the tumor cell elution chamber is provided with an eluent conduit; the red blood cell collecting chamber is positioned above the tumor cell eluting chamber, a push rod is arranged in the red blood cell collecting chamber, one end of the push rod is connected with a piston, the other end of the push rod extends out of the red blood cell collecting chamber, and a red blood cell discharging pipe is arranged on one side of the red blood cell collecting chamber; the hydrops collection chamber is located the opposite side of red blood cell collection chamber, one side that the chamber was collected to the hydrops is equipped with hydrops eduction tube.
2. The device according to claim 1, wherein the device further comprises: and air blowing pipes communicated with the tumor cell eluting chamber are respectively arranged above and below the eluent conduit, and the end parts of the air blowing pipes are connected with extrusion air bags.
3. The device according to claim 1, wherein the device further comprises: the pore diameter of the first filter membrane layer is 6-10 μm.
4. The device according to claim 1, wherein the device further comprises: the pore diameter of the second filter membrane layer is 2-4 μm.
5. The device according to claim 1, wherein the device further comprises: and a cleaning solution guide pipe is also arranged on one side of the red blood cell collecting chamber.
6. The device according to claim 1, wherein the device further comprises: the bottom of the tumor cell elution chamber is V-shaped.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920819754.8U CN210204746U (en) | 2019-05-31 | 2019-05-31 | Circulating tumor cell collecting device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920819754.8U CN210204746U (en) | 2019-05-31 | 2019-05-31 | Circulating tumor cell collecting device |
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| Publication Number | Publication Date |
|---|---|
| CN210204746U true CN210204746U (en) | 2020-03-31 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201920819754.8U Expired - Fee Related CN210204746U (en) | 2019-05-31 | 2019-05-31 | Circulating tumor cell collecting device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111733072A (en) * | 2020-06-08 | 2020-10-02 | 中国科学院宁波工业技术研究院慈溪生物医学工程研究所 | Circulating tumor cell screening and separating device and method and application |
-
2019
- 2019-05-31 CN CN201920819754.8U patent/CN210204746U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111733072A (en) * | 2020-06-08 | 2020-10-02 | 中国科学院宁波工业技术研究院慈溪生物医学工程研究所 | Circulating tumor cell screening and separating device and method and application |
| CN111733072B (en) * | 2020-06-08 | 2023-06-30 | 中国科学院宁波工业技术研究院慈溪生物医学工程研究所 | Circulating tumor cell screening and separating device, method and application |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200331 |
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| CF01 | Termination of patent right due to non-payment of annual fee |