CN213624139U - Simple and easy cell collection filter equipment - Google Patents
Simple and easy cell collection filter equipment Download PDFInfo
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- CN213624139U CN213624139U CN202022009095.7U CN202022009095U CN213624139U CN 213624139 U CN213624139 U CN 213624139U CN 202022009095 U CN202022009095 U CN 202022009095U CN 213624139 U CN213624139 U CN 213624139U
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Abstract
The utility model provides a simple cell collecting and filtering device, which comprises a piston cylinder, wherein the piston cylinder comprises an end cover and a rear cylinder body which are arranged in the front and the rear, the end cover is connected with the rear cylinder body by screw thread, and a sealing ring is arranged at the joint of the end cover and the rear cylinder body; a liquid inlet extending forwards is formed in the center of the end cover, a filter screen is installed in the liquid inlet, and a valve is installed on the liquid inlet; a cell filter sieve is clamped and fixed between the end cover and the rear cylinder body, and the meshes of the cell filter sieve are smaller than those of the filter screen; the rear cylinder body is positioned at the rear part of the cell filter sieve and is provided with a liquid outlet, and a valve is arranged on the liquid outlet; the piston is arranged in the piston cylinder and is connected with the rear cylinder body in a sliding way; the piston also comprises a push rod, and the push rod is connected with the rear end part of the piston. This patent realizes going on in step of the transfer and the filterable of liquid, has portably operated, through the cooperation of piston and piston cylinder, and then realizes the vacuum aspiration of cell culture liquid, filters for traditional gravity, has improved filtration efficiency.
Description
Technical Field
The utility model relates to the technical field of machinery, concretely relates to cell filtering device.
Background
Cell primary culture refers to a culture stage in which cells, tissues and organs are directly removed from the body and cultured until they are before passage. In addition to the cells, the cell culture solution obtained after the primary culture of the cells generally has original tissues, organs, cell masses, and culture solution that are not completely dispersed, so that it is necessary to first subject the mixture solution after the completion of the culture to preliminary filtration.
There are various ways of preliminary filtration and separation, and the most commonly used separation method is cell filtration.
When the cell culture solution is filtered, the cell culture solution to be filtered is often transferred to a cell filter for filtering through devices such as a pipette, and the transferring and filtering are performed step by step, so that the operation is complex.
In addition, the cell filter of current cell filter through its specific pore size's that has cell filter sieve, filters cell culture liquid, and the gravity that cell filter sieve adopted filters, and filtration efficiency is lower, and speed is slower, and the cell in the in-process cell culture liquid pollutes easily, and the mesh is stopped up easily.
SUMMERY OF THE UTILITY MODEL
Problem to prior art exists, the utility model provides a filter equipment is collected to simple and easy cell to solve current move liquid and filter substep complex operation, and the technical problem that filtration efficiency is low.
The technical scheme of the utility model is that: the simple cell collecting and filtering device is characterized by comprising a piston cylinder, wherein the piston cylinder comprises an end cover and a rear cylinder body which are arranged in front and at back, the end cover is in threaded connection with the rear cylinder body, and a sealing ring is arranged at the joint of the end cover and the rear cylinder body;
a liquid inlet extending forwards is formed in the center of the end cover, a filter screen is installed in the liquid inlet, and a valve or a plugging cover is installed on the liquid inlet;
a cell filter sieve is clamped and fixed between the end cover and the rear cylinder body, and the meshes of the cell filter sieve are smaller than those of the filter screen;
the rear cylinder body is positioned behind the cell filter sieve and is provided with a liquid outlet, and the liquid outlet is provided with a valve or a blocking cover;
the piston is arranged in the piston cylinder and is connected with the rear cylinder body in a sliding manner;
the piston is characterized by further comprising a push rod, and the push rod is connected with the rear end portion of the piston.
This patent is through optimizing filter equipment's structure, realizes going on in step of the transfer and the filterable of liquid, has portably operated, through the cooperation of piston and piston cylinder, and then realizes the vacuum aspiration of cell culture liquid, filters for traditional gravity, has improved filtration efficiency.
Further preferably, the liquid inlet is connected with a liquid suction nozzle through threads.
Is convenient for meeting different liquid absorption requirements. And replacing different liquid suction nozzles.
Further preferably, the cell filter sieve is bent, and a front end portion of the piston is matched with a curvature of the cell filter sieve.
Through the bending of the cell filter screen, the filter area is convenient to increase, and the filter effect is improved.
Further preferably, the cell filter sieve comprises a filter side cylinder and a filter disc, the rear end of the filter side cylinder is fixedly connected with the filter disc, and the front end of the filter side cylinder is provided with a flanging extending outwards;
the flanging is clamped between the end cover and the rear cylinder body;
holes for filtering are formed in the filtering side barrel and the filtering disc;
the front end of the piston is provided with a cylindrical groove for embedding the filtering side barrel and the filtering disc.
By optimizing the structure of the cell filter sieve, the filtering efficiency is convenient to increase.
Preferably, the liquid inlet is provided with a valve, and the valve on the liquid inlet is a one-way valve leading the flow direction from the liquid inlet into the piston cylinder;
the valve on the liquid outlet is a one-way valve which is guided out from the piston cylinder in a flow guiding direction.
The automatic opening of the liquid inlet and the liquid outlet is convenient to realize in the reciprocating motion process of the piston, and the complexity of manual operation is avoided.
Further preferably, the liquid outlet is detachably connected with a conduit, and a pipe buckle or a pipe clamp for fixing the conduit is arranged on the side wall of the rear cylinder.
When needing to discharge liquid, the catheter is put into a centrifuge tube or an EP tube to collect cell suspension for next operation.
Further preferably, the liquid outlet is formed in the front end portion or the rear end portion of the rear cylinder.
When the liquid outlet is arranged at the front end part of the rear cylinder body, the sample is pushed out by the forward pushing of the piston.
When the liquid outlet is arranged at the rear end part of the rear barrel, after sample suction is finished, the piston continuously moves backwards, and when the piston is positioned behind the liquid outlet, the blocking cover or the valve at the liquid outlet is opened, so that a sample is led out from the liquid outlet.
Drawings
Fig. 1 is a cross-sectional view of the present invention;
FIG. 2 is a cross-sectional view of the cell filter screen of the present invention in a bent shape;
FIG. 3 is a cross-sectional view of the liquid outlet of the present invention disposed at the rear end of the rear cylinder;
FIG. 4 is a schematic structural view of the bent cell filter screen of the present invention.
In the figure: 1 is an end cover, 2 is a rear cylinder body, 3 is a piston, 4 is a push rod, 5 is a liquid inlet, 6 is a liquid outlet, 7 is a filter screen, 8 is a cell filter screen, 81 is a flanging, 82 is a filter side cylinder, and 83 is a filter disc.
Detailed Description
The present invention will be further described with reference to the accompanying drawings.
Referring to fig. 1 to 4, a simple cell collecting and filtering device comprises a piston cylinder, wherein the piston cylinder comprises an end cover 1 and a rear cylinder body 2 which are arranged in front and back, the end cover 1 is in threaded connection with the rear cylinder body 2, and a sealing ring is arranged at the joint of the end cover 1 and the rear cylinder body 2; a liquid inlet 5 extending forwards is formed in the center of the end cover 1, a filter screen 7 is installed in the liquid inlet 5, and a valve or a blocking cover is installed on the liquid inlet 5; a cell filter sieve 8 is clamped and fixed between the end cover 1 and the rear cylinder body 2, and the meshes of the cell filter sieve 8 are smaller than those of the filter screen 7; a liquid outlet 6 is arranged on the rear part of the cell filter sieve 8 of the rear cylinder 2, and a valve or a plugging cover is arranged on the liquid outlet 6; the piston 3 is arranged in the piston cylinder, and the piston 3 is connected with the rear cylinder body 2 in a sliding manner; and the push rod 4 is connected with the rear end part of the piston 3. This patent is through optimizing filter equipment's structure, realizes going on in step of the transfer and the filterable of liquid, has portably operated, through the cooperation of piston 3 with a piston cylinder, and then realizes the vacuum aspiration of cell culture liquid, filters for traditional gravity, has improved filtration efficiency.
The liquid inlet 5 is connected with a liquid suction nozzle through threads. Is convenient for meeting different liquid absorption requirements. And replacing different liquid suction nozzles.
The filter screen is detachably connected with the end cover. The end cover can be rotationally clamped with the filter screen. Or the filter screen is provided with a flanging extending backwards, and the flanging is in threaded connection with the front end of the liquid inlet. The filter screen is convenient to replace. The filter screen is used to stop large tissues that are not digested.
The outer edge of the cell filter sieve 8 is sleeved with a sealing ring. There is a gap between the cell filter sieve and the end cover.
Aiming at the aperture of the filter screen and the cell filter screen, the technical personnel in the field can adjust according to different filter requirements, so the patent is not limited.
Referring to fig. 4, the cell filter sieve is bent, and the front end of the piston 3 is matched with the curvature of the cell filter sieve. Through the bending of the cell filter screen, the filter area is convenient to increase, and the filter effect is improved. The cell filter sieve comprises a filter side cylinder 82 and a filter disc 83, the rear end of the filter side cylinder 82 is fixedly connected with the filter disc 83, and the front end of the filter side cylinder 82 is provided with a flanging 81 extending outwards; the flange 81 is clamped between the end cover 1 and the rear cylinder 2; holes for filtering are formed in the filtering side cylinder 82 and the filtering disc 83; the front end of the piston 3 is provided with a cylindrical groove for embedding the filtering side barrel and the filtering disc. By optimizing the structure of the cell filter sieve, the filtering efficiency is convenient to increase. The inner diameter of the cylindrical groove is matched with that of the filtering side cylinder. The axial length of the cylindrical groove is matched with that of the filtering side cylinder. The center of the end cap matches the curvature of the cell filter screen. The center of the end cover is concave. Reducing the degree of liquid inlet protrusion. The control device occupies the whole volume and is easy to package.
The liquid outlet 6 is arranged at the front end part of the rear cylinder body 2. When the liquid outlet 6 is arranged at the front end part of the rear cylinder body 2, the sample is pushed out by the forward pushing of the piston 3.
The liquid outlet 6 is arranged at the rear end part of the rear cylinder body 2. When liquid outlet 6 was seted up at the back tip of back barrel 2, after the appearance of inhaling, piston 3 constantly moves backward, when piston 3 was located the rear of liquid outlet 6, opens the shutoff lid or the valve of liquid outlet 6 department, derives the sample from liquid outlet 6.
The liquid inlet 5 is provided with a valve, and the valve on the liquid inlet 5 is a one-way valve leading the flow direction from the liquid inlet 5 into the piston cylinder; the liquid outlet 6 is provided with a valve, and the valve on the liquid outlet 6 is a one-way valve which leads the flow direction out of the piston cylinder. The automatic opening of the liquid inlet 5 and the liquid outlet 6 is realized conveniently in the reciprocating motion process of the piston 3, and the complexity of manual operation is avoided.
The liquid outlet 6 is detachably connected with a conduit, and the side wall of the rear cylinder body 2 is provided with a pipe buckle or a pipe clamp for fixing the conduit. When needing to discharge liquid, the catheter is put into a centrifuge tube or an EP tube to collect cell suspension for next operation.
The use process of the device is as follows:
1. and (4) extending the liquid inlet into the sample liquid to be filtered.
2. The push rod is used for pulling the piston to generate negative pressure, and the sample liquid to be filtered is sucked.
3. After the sample suction is finished, the liquid inlet is sealed, the push rod is pushed, and the cell suspension is discharged from the liquid outlet.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of improvements and decorations can be made without departing from the principle of the present invention, and these improvements and decorations should also be regarded as the protection scope of the present invention.
Claims (7)
1. The simple cell collecting and filtering device is characterized by comprising a piston cylinder, wherein the piston cylinder comprises an end cover and a rear cylinder body which are arranged in front and at back, the end cover is in threaded connection with the rear cylinder body, and a sealing ring is arranged at the joint of the end cover and the rear cylinder body;
a liquid inlet extending forwards is formed in the center of the end cover, a filter screen is installed in the liquid inlet, and a valve or a plugging cover is installed on the liquid inlet;
a cell filter sieve is clamped and fixed between the end cover and the rear cylinder body, and the meshes of the cell filter sieve are smaller than those of the filter screen;
the rear cylinder body is positioned behind the cell filter sieve and is provided with a liquid outlet, and the liquid outlet is provided with a valve or a blocking cover;
the piston is arranged in the piston cylinder and is connected with the rear cylinder body in a sliding manner;
the piston is characterized by further comprising a push rod, and the push rod is connected with the rear end portion of the piston.
2. The simple cell collecting and filtering device as claimed in claim 1, wherein: the liquid inlet is connected with a liquid suction nozzle through threads.
3. The simple cell collecting and filtering device as claimed in claim 1, wherein: the cell filter screen is bent, and the front end part of the piston is matched with the curvature of the cell filter screen.
4. The simple cell collecting and filtering device as claimed in claim 1, wherein: the cell filter sieve comprises a filter side cylinder and a filter disc, the rear end of the filter side cylinder is fixedly connected with the filter disc, and the front end of the filter side cylinder is provided with a flanging extending outwards;
the flanging is clamped between the end cover and the rear cylinder body;
holes for filtering are formed in the filtering side barrel and the filtering disc;
the front end of the piston is provided with a cylindrical groove for embedding the filtering side barrel and the filtering disc.
5. The simple cell collecting and filtering device as claimed in claim 1, wherein: the valve on the liquid inlet is a one-way valve leading the flow direction from the liquid inlet into the piston cylinder;
the valve on the liquid outlet is a one-way valve which is guided out from the piston cylinder in a flow guiding direction.
6. The simple cell collecting and filtering device as claimed in claim 1, wherein: the liquid outlet can be dismantled and be connected with a pipe, be equipped with on the lateral wall of back barrel and be used for fixing the pipe is buckled or the pipe clamp of pipe.
7. The simple cell collecting and filtering device as claimed in claim 1, wherein: the liquid outlet is arranged at the front end part or the rear end part of the rear cylinder body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022009095.7U CN213624139U (en) | 2020-09-15 | 2020-09-15 | Simple and easy cell collection filter equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022009095.7U CN213624139U (en) | 2020-09-15 | 2020-09-15 | Simple and easy cell collection filter equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213624139U true CN213624139U (en) | 2021-07-06 |
Family
ID=76648876
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022009095.7U Active CN213624139U (en) | 2020-09-15 | 2020-09-15 | Simple and easy cell collection filter equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213624139U (en) |
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2020
- 2020-09-15 CN CN202022009095.7U patent/CN213624139U/en active Active
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