CN204939464U - Cellular segregation collector - Google Patents
Cellular segregation collector Download PDFInfo
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- CN204939464U CN204939464U CN201520683199.2U CN201520683199U CN204939464U CN 204939464 U CN204939464 U CN 204939464U CN 201520683199 U CN201520683199 U CN 201520683199U CN 204939464 U CN204939464 U CN 204939464U
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- waste liquid
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- collecting chamber
- collector
- filter membrane
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Abstract
The utility model provides a kind of cellular segregation collector for centrifugal device, it comprise be arranged on centrifugal device far-end and collect under centrifugal action target cell collecting chamber, the waste liquid chamber passed through for isolated waste liquid, be arranged at and be blocked between collecting chamber and waste liquid chamber and by target cell in collecting chamber and make the waste liquid of separation pass through and enter the filter membrane in waste liquid chamber; Collecting chamber is provided with the fluid inlet entering collecting chamber for cell suspending liquid, waste liquid chamber is provided with the liquid outlet that the waste liquid of separation is discharged; The diameter of the filter opening of filter membrane is less than the diameter of target cell.By cellular segregation collector of the present utility model, structure is simple, easy to operate, can isolated cell rapidly and accurately, is separated in totally-enclosed system and carries out, not easily cause damage and the pollution of cell; And target cell can be deposited in acquisition cavity preferably, can not block filter membrane, makes separation efficiency improve.
Description
Technical field
The utility model belongs to medical instruments field, particularly a kind of cellular segregation collector of collecting by filtration target cell.
Background technology
Cell therapy refers to the characteristic utilizing some to have the cell of specific function, after employing biological engineering method is obtained and/or is processed by amplification in vitro, specific culture etc., make these cells have enhancing immunity, kill the therapeutic efficiency such as pathogenic agent and tumour cell, promotion tissue and organ regeneration and physical recovery, thus reach the object of disease therapy.Cell therapy is T&B, is widely used in tumour, hepatic diseases at present, goes the every field such as scar, and have vast potential for future development.
The most basic problem of cell therapy is exactly first will isolate target cell.In prior art, usual employing whizzer realizes cellular segregation, celliferous nutrient solution is put into centrifuge tube in batches centrifugal, then take out supernatant liquor, the centrifugal several of injection balance liquid is to wash down nutrient chemical, and whole process is time-consuming, effort, and easy contamination of cells, repeatedly centrifugally also cause damage to cell, cell harvesting rate reduces.
For this reason, be necessary to provide a kind of cellular segregation collector preferably.
Utility model content
The purpose of this utility model be to provide a kind of can sharp separation collect required target cell by centrifugal action, easy and simple to handle and the cell injury produced in sepn process can be alleviated, not easily cause the cellular segregation collector of cell contamination.
For this reason, a kind of cellular segregation collector for centrifugal device in the utility model, is characterized in that: comprise be arranged on described centrifugal device far-end and collect under centrifugal action described target cell collecting chamber, the waste liquid chamber passed through for isolated waste liquid, be arranged at and be blocked between described collecting chamber and waste liquid chamber and by described target cell in described collecting chamber and make the described waste liquid of separation pass through and enter the filter membrane in described waste liquid chamber; Described collecting chamber is provided with the fluid inlet entering described collecting chamber for described cell suspending liquid, described waste liquid chamber is provided with the liquid outlet that the waste liquid of separation is discharged; The diameter of the filter opening of described filter membrane is less than the diameter of described target cell.
Preferably, the inner chamber of described collecting chamber is towards the diminishing shape in direction away from described filter membrane.
Preferably, the inner chamber of described collecting chamber is back taper chamber, and described filter membrane is circular.
Preferably, the outside of described collecting chamber is provided with base.
Preferably, the diameter of the filter opening of described filter membrane is 1-30um.
Relative to prior art, the utility model cellular segregation collector structure is simple, easy to operate, can isolated cell rapidly and accurately; And whole sepn process is carried out under full closeding state, not easily cause damage and the pollution of cell; And target cell can be deposited in acquisition cavity preferably, can not block filter membrane, and then greatly improve separation efficiency.
Accompanying drawing explanation
Fig. 1 is the one-piece construction schematic diagram of centrifugal device;
Fig. 2 is the structural representation of the cell filtration collector of the first embodiment of the present utility model;
Fig. 3 is the structural representation of the cell filtration collector of the second embodiment of the present utility model.
Embodiment
Describe cell separation apparatus of the present invention preferably embodiment in detail below in conjunction with accompanying drawing, but the present invention is not limited to these embodiments.
Fig. 1 is the one-piece construction schematic diagram of centrifugal device, and as shown in Figure 1, cellular segregation collector of the present utility model is positioned at centrifugal device both sides; Fig. 2 is the structural representation of cellular segregation collector of the present utility model, as shown in Figure 2, cellular segregation collector on centrifugal device of the present utility model, comprise be arranged on centrifugal device far-end and collect under centrifugal action target cell collecting chamber 13 and the waste liquid chamber 14 passed through for isolated waste liquid, be arranged at and be blocked between collecting chamber 13 and waste liquid chamber 14 and by target cell in collecting chamber 13 and make the waste liquid of separation pass through and enter the filter membrane 15 in waste liquid chamber 14;
Collecting chamber 13 is provided with the fluid inlet 11 entering described collecting chamber for described cell suspending liquid, waste liquid chamber 14 is provided with the liquid outlet 12 that the waste liquid of separation is discharged;
The diameter of the filter opening of filter membrane 15 is less than the diameter of described target cell;
When carrying out centrifuging, cell suspending liquid enters collecting chamber 13 by fluid inlet 11, in cell suspending liquid, moisture content and biological particles and biomolecules are entered waste liquid chamber 14 due to liquid stream pressure by filter membrane 15 and are discharged by liquid outlet 12, and target cell is stopped by filter membrane 15 and throws away filter membrane 15 under the influence of centrifugal force and fall to collecting chamber 13.
In this cell filtration collector of the present utility model, target cell not easily blocks filter membrane owing to can throw away described filter membrane under the effect of centrifugal force, thus the efficiency of centrifugal filtration process is improved.
As shown in Figure 1, when this cell filtration collector is installed on centrifugal dynamic filter, fluid inlet 11 and acquisition cavity 13 are positioned at the far-end of filtration unit centrifuge arm, liquid outlet 12 and waste liquid chamber 14 are positioned at the near-end of centrifuge arm, that is to say that the position of acquisition cavity 13 is compared with the position in waste liquid chamber 14, the luxuriant central arm of acquisition cavity 13 is far away than waste liquid chamber 14.Like this, liquid flows to liquid outlet 12 from fluid inlet 11, is to flow from away from centrifuge arm to nearly centrifuge arm direction; Like this, when cell suspending liquid arrives acquisition cavity 13, while cell is isolated in acquisition cavity 13 by filter membrane 15, acquisition cavity 13 can be fallen to away from filter membrane 15 under the influence of centrifugal force, thus can not filter opening be blocked, filtration procedure be continued smooth and easy.Moisture content and the waste liquid such as biological particles and biomolecules are then because liquid stream pressure enters waste liquid chamber 14 by filter membrane 15.Terminate centrifugal after, operator directly can take this cell filtration collector away and obtain useful cell.
Filter membrane 15 is hydrophilic filter film, and it adopts polyolefins or polyamide polymer to make.Preferably, filter membrane is made up of polyethylene or cellulose mixture or PE material or nylon material.The filter opening of filter membrane 15 is less than the size of the cell wanting separation and Extraction, thus the cell that must extract is isolated be deposited in acquisition cavity 13, and moisture content and other biomolecules flow to waste liquid chamber 14, then flow out from liquid outlet 12.Because general somatic size is 5-30um, particularly, the diameter of the filter opening of filter membrane 15 is less than 5um.Preferably, the diameter of the filter opening of filter membrane is 1-30um; More preferably, the diameter of filter opening is 3um-5um.
The inner chamber of described collecting chamber is towards the diminishing shape in direction away from described filter membrane.
As shown in Figure 2, cellular segregation collector can be formed as the inner chamber of collecting chamber towards the diminishing shape in direction away from filter membrane, particularly, when cellular segregation collector is arranged on centrifugal dynamic filter, the collecting chamber of cellular segregation collector is away from (namely towards on the direction away from described filter membrane) on the direction of centrifuge arm, and its lumenal cross-section is long-pending to be reduced gradually.The inner chamber of such as collecting chamber can be formed as back taper chamber, and filter membrane is formed as circular.Due to farthest away from centrifugal shaft position-namely the most advanced and sophisticated centrifugal force of back taper is maximum, cell can be made like this to be deposited on collecting chamber better, make its be not easy blocking to filter membrane hole place.
As shown in Figure 3, on the basis of embodiment one, a base can be set up in the collecting chamber outside of the utility model cellular segregation collector, makes cellular segregation collector complete in centrifugal collection and to be positioned over reposefully horizontal plane after centrifugal device takes off.
Adopt cellular segregation collector of the present invention, in centrifugal process, operate very easy, target cell can be gathered in easily in collecting chamber and not block filter membrane, and waste liquid can effectively be discharged, and separation efficiency is very remarkable.After centrifugal end, operator directly can take off this cellular segregation collector from cell separation apparatus, it is all now useful target cell in cellular segregation collector, such device can make centrifugal process very convenient, do not need to carry out many times centrifugally operated repeatedly, isolated target cell also can not be polluted simultaneously.
Content described in this specification sheets embodiment is only enumerating the way of realization of inventive concept; protection scope of the present invention should not be regarded as being only limitted to the specific form that embodiment is stated, protection scope of the present invention also and conceive the equivalent technologies means that can expect according to the present invention in those skilled in the art.
Claims (5)
1., for a cellular segregation collector for centrifugal device, it is characterized in that: comprise be arranged on described centrifugal device far-end and collect under centrifugal action target cell collecting chamber, the waste liquid chamber passed through for isolated waste liquid, be arranged at and be blocked between described collecting chamber and waste liquid chamber and by described target cell in described collecting chamber and make the described waste liquid of separation pass through and enter the filter membrane in described waste liquid chamber;
Described collecting chamber is provided with the fluid inlet entering described collecting chamber for described cell suspending liquid, described waste liquid chamber is provided with the liquid outlet that the waste liquid of separation is discharged;
The diameter of the filter opening of described filter membrane is less than the diameter of described target cell.
2. cellular segregation collector as claimed in claim 1, is characterized in that: the inner chamber of described collecting chamber is towards the diminishing shape in direction away from described filter membrane.
3. cellular segregation collector as claimed in claim 2, is characterized in that: the inner chamber of described collecting chamber is back taper chamber, and described filter membrane is circular.
4. cellular segregation collector as claimed any one in claims 1 to 3, is characterized in that: the outside of described collecting chamber is provided with base.
5. cellular segregation collector as claimed in claim 1, is characterized in that: the diameter of the filter opening of described filter membrane is 1-30um.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520683199.2U CN204939464U (en) | 2015-09-02 | 2015-09-02 | Cellular segregation collector |
| US15/230,516 US10258927B2 (en) | 2012-12-14 | 2016-08-08 | Centrifugal dynamic filtering apparatus and cell separation system using same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520683199.2U CN204939464U (en) | 2015-09-02 | 2015-09-02 | Cellular segregation collector |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204939464U true CN204939464U (en) | 2016-01-06 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520683199.2U Active CN204939464U (en) | 2012-12-14 | 2015-09-02 | Cellular segregation collector |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204939464U (en) |
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2015
- 2015-09-02 CN CN201520683199.2U patent/CN204939464U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |