CN211079112U

CN211079112U - Circulating tumor is trapping apparatus for cell

Info

Publication number: CN211079112U
Application number: CN201920817686.1U
Authority: CN
Inventors: 宋明旭; 来锦云; 蒋文君
Original assignee: Affiliated Hospital of Jiangnan University
Current assignee: Affiliated Hospital of Jiangnan University
Priority date: 2019-05-31
Filing date: 2019-05-31
Publication date: 2020-07-24
Anticipated expiration: 2029-05-31

Abstract

The utility model relates to the technical field of medical auxiliary appliances, in particular to a capture device for circulating tumor cells, which comprises a capture device body, wherein the top of the capture device body is provided with a blood inlet, the interior of the capture device body is divided into a first cavity, a second cavity and a third cavity through a filter membrane layer, a plurality of telescopic balls are arranged in the first cavity, the telescopic balls are connected with an inflation tube through telescopic tubes, the other side of the first cavity is provided with an eluent conduit, and the top of the first cavity is provided with a tumor cell outlet; one side of the second chamber is provided with a plurality of air blowing pipes communicated with the second chamber, and one side of the second chamber is provided with a red blood cell delivery pipe; one side of the third chamber is provided with a cleaning liquid guide pipe, and one side of the bottom of the third chamber is provided with a effusion outlet. The capture device is more beneficial to the elution and capture of the tumor cells.

Description

Circulating tumor is trapping apparatus for cell

Technical Field

The utility model relates to the technical field of medical equipment, especially, relate to a circulating tumor is trapping apparatus for cell.

Background

Circulating Tumor Cells (CTCs) are a general name of various tumor cells existing in peripheral blood, and fall off from solid tumor focuses (primary focuses and metastatic focuses) due to spontaneous or diagnosis and treatment operations, most CTCs are subjected to apoptosis or phagocytosis after entering the peripheral blood, and a few CTCs can escape and develop into the metastatic focuses, so that the death risk of malignant tumor patients is greatly increased. The medical personnel in the prior art usually adopt a collection device with puncture to extract the circulating tumor cells of a patient, then transfer the circulating tumor cells into a capture device for subsequent elution, wash out interfering components such as red blood cells and the like, and then detect the eluted collection; the existing capture device is internally provided with a filter membrane layer, red blood cells, effusion and other components are separated through the filter membrane layer, but blood carrying tumor cells, red blood cells and other components enters the capture device in a static state, and after the tumor cells block the interception micropores of the filter membrane layer, the red blood cells, effusion and other components cannot be separated from the tumor capture material, so that the capture of the tumor cells is influenced finally.

SUMMERY OF THE UTILITY MODEL

The utility model discloses the technical problem that will solve is: aiming at the defects of the prior art, the capturing device for the circulating tumor cells is provided, and can promote the mobility of components such as tumor cells, effusion, red blood cells and the like, and is convenient for capturing the tumor cells.

In order to solve the technical problem, the technical scheme of the utility model is that:

a capturing device for circulating tumor cells comprises a capturing device body, wherein a blood inlet is formed in the top of the capturing device body, the inside of the capturing device body is divided into a first chamber, a second chamber and a third chamber through a filter membrane layer, a plurality of telescopic balls are arranged in the first chamber, the telescopic balls are connected with an inflation tube through telescopic tubes, an eluent conduit is arranged on the other side of the first chamber, and a tumor cell outlet is formed in the top of the first chamber; a plurality of air blowing pipes communicated with the second chamber are arranged on one side of the second chamber, and an erythrocyte eduction pipe is arranged on one side of the second chamber; one side of the third chamber is provided with a cleaning liquid guide pipe, and one side of the bottom of the third chamber is provided with a effusion outlet.

As an improved technical scheme, the end part of the inflation tube is connected with a squeezing air bag.

As an improved technical scheme, an inflatable air bag is connected to the end part of the air blowing pipe.

As an improved technical solution, the filtration membrane layer includes a first filtration membrane layer and a second filtration membrane layer, the first filtration membrane layer is located between the first chamber and the second chamber, and the second filtration membrane layer is located between the second chamber and the third chamber.

As a modified technical scheme, the third chamber is in a funnel shape.

As an improved technical scheme, the interception aperture of the first filtering membrane layer is 5-8 um.

As an improved technical scheme, the interception aperture of the second filtering membrane layer is 2-4 um.

After the technical scheme is adopted, the beneficial effects of the utility model are that:

the trapping device for circulating tumor cells comprises a trapping device body, wherein a blood inlet is formed in the top of the trapping device body, the interior of the trapping device body is divided into a first chamber, a second chamber and a third chamber through a filter membrane layer, a plurality of telescopic balls are arranged in the first chamber, the telescopic balls are connected with an inflation tube through the telescopic tubes, an eluent guide tube is arranged on the other side of the first chamber, and a tumor cell outlet is formed in the top of the first chamber; one side of the second chamber is provided with a plurality of air blowing pipes communicated with the second chamber, and one side of the second chamber is provided with a red blood cell delivery pipe; one side of the third chamber is provided with a cleaning liquid guide pipe, and one side of the bottom of the third chamber is provided with a effusion outlet. Blood carrying tumor cells enters the capturing device body from a blood inlet and then is firstly retained in the first cavity, the telescopic ball is inflated through the inflation tube and the telescopic tube in order to increase the fluidity of the blood, the telescopic tube and the telescopic ball are inflated and then move telescopically in the first cavity, and meanwhile, the fluidity of the blood is increased, components such as effusion, red blood cells with small diameters and the like pass through the first filtering membrane layer to enter the second cavity, the tumor cells are retained in the first cavity, an eluent is conveyed into the first cavity through the eluent conduit, and the tumor cells are discharged from the tumor cell outlet and then are used for later-stage detection; inside back of components such as hydrops and red blood cell got into the second cavity, blow in to the second cavity through the gas blow pipe, the mobile shape of hydrops and red blood cell has been increased, red blood cell is held back to the second cavity inside, discharge components such as red blood cell through the red blood cell contact tube, the hydrops gets into inside the third cavity, discharge the hydrops through the hydrops discharge port, carry the washing liquid to the third cavity inside through the washing liquid pipe at last, the washing liquid passes inside filter membrane layer gets into second cavity and first cavity, realized first, second and third cavity's washing. The capture device increases the fluidity of blood, is more beneficial to capturing tumor cells, and realizes the effective separation of components such as red blood cells.

The end of the inflation tube is connected with an extrusion air bag. Medical personnel through by the spaced extrusion gasbag, gaseous along the gas tube entering flexible intraduct, at last along flexible intraduct entering flexible ball. Medical personnel's spaced extrusion gasbag for the flexible ball is at first cavity inside concertina movement, thereby promotes the mobility of blood. The structure is reasonable in design and simple in operation, and is more beneficial to capturing tumor cells.

The end part of the air blowing pipe is connected with an inflatable air bag. Medical personnel inflate the gasbag through the extrusion, and inside gas got into the second chamber along the gas blow pipe, blow hydrops and red blood cell and flow in the second chamber, and above-mentioned design more helps the separation of hydrops and red blood cell.

Since the filtration membrane layers include a first filtration membrane layer and a second filtration membrane layer, the first filtration membrane layer is located between the first chamber and the second chamber, and the second filtration membrane layer is located between the second chamber and the third chamber. The first filtering membrane layer and the second filtering membrane layer respectively realize the interception of components such as tumor cells, red blood cells and the like, and are favorable for eluting and capturing the tumor cells in blood.

Due to the funnel shape of the third chamber. The third chamber is designed to be funnel-shaped, so that collection and discharge of accumulated liquid are facilitated.

The interception aperture of the first filter membrane layer is 5-8 um. The first filtering membrane layer with the cut-off pore size is used for realizing the cut-off of tumor cells.

The interception aperture of the second filter membrane layer is 2-4 um. The second filtering membrane layer with the interception pore size realizes the interception of components such as red blood cells.

Drawings

FIG. 1 is a schematic structural view of a capturing device for circulating tumor cells according to the present invention;

the device comprises a capturing device body, a blood inlet, a first cavity, an eluent conduit, a tumor cell outlet, a.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

A capture device for circulating tumor cells is shown in figure 1 and comprises a capture device body 1, wherein a blood inlet 10 is arranged at the top of the capture device body 1, the interior of the capture device body 1 is divided into a first chamber 11, a second chamber 12 and a third chamber 13 through a filter membrane layer, a plurality of telescopic balls 2 (formed by processing elastic plastics) are respectively arranged at two sides of the interior of the first chamber 11, the telescopic balls 2 are connected with an inflation tube 4 (formed by processing elastic plastics) through a telescopic tube 3, the end part of the inflation tube 4 is connected with an extrusion air bag 5, an eluent conduit 110 is arranged at the other side of the first chamber 11, and a tumor cell outlet 111 is arranged at the top of the first chamber 11; a plurality of air blowing pipes 120 communicated with the second chamber 12 are arranged at one side of the second chamber 12, an air inflation bag 121 is connected at the end part of the air blowing pipe 120, and an erythrocyte eduction pipe 122 is arranged at one side of the second chamber 12; one side of the third chamber 13 is provided with a cleaning solution conduit 130, and one side of the bottom of the third chamber (funnel-shaped) 13 is provided with a liquid loading outlet 131.

Blood carrying tumor cells enters the capturing device body from a blood inlet and then is firstly retained in the first chamber, medical staff pass through the spaced extrusion air bag, gas enters the telescopic pipe along the inflation pipe and finally enters the telescopic ball along the telescopic pipe, the telescopic ball makes telescopic motion in the first chamber, the mobility of the blood is increased, components such as effusion and red blood cells with small diameters pass through the first filtering membrane layer to enter the second chamber, the tumor cells are retained in the first chamber, an eluent is conveyed into the first chamber through the eluent guide pipe, and the tumor cells are discharged from the tumor cell outlet and then are used for later-stage detection; medical personnel aerify the gasbag through the extrusion, it is inside that gas gets into the second cavity along the gas blow pipe, blow hydrops and red blood cell and flow at the second cavity is inside, after components such as hydrops and red blood cell get into the second cavity inside, blow in the second cavity through the gas blow pipe, the mobile shape of hydrops and red blood cell has been increased, red blood cell is held back to the second cavity inside, discharge components such as red blood cell through red blood cell contact tube, the hydrops gets into the third cavity inside, discharge the hydrops through the hydrops discharge port, carry the washing liquid to the third cavity inside through the washing liquid pipe at last, the washing liquid passes inside filter membrane layer gets into second cavity and first cavity, realized first, the washing of second and third cavity. The capture device increases the fluidity of blood, is more beneficial to capturing tumor cells, and realizes the effective separation of components such as red blood cells.

Wherein the filtration membrane layer comprises a first filtration membrane layer 6 (the cut-off pore size is 5-8um) and a second filtration membrane layer 7 (the cut-off pore size is 2-4um), the first filtration membrane layer 6 is located between the first chamber 11 and the second chamber 12, and the second filtration membrane layer 7 is located between the second chamber 12 and the third chamber 13. The first filtering membrane layer and the second filtering membrane layer respectively realize the interception of components such as tumor cells, red blood cells and the like, and are favorable for eluting and capturing the tumor cells in blood.

The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modifications, equivalents, improvements and the like made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.

Claims

1. A capturing device for circulating tumor cells, characterized in that: the device comprises a capturing device body, wherein a blood inlet is formed in the top of the capturing device body, the interior of the capturing device body is divided into a first chamber, a second chamber and a third chamber through a filter membrane layer, a plurality of telescopic balls are respectively arranged on two sides of the interior of the first chamber, the telescopic balls are connected with an inflation tube through telescopic tubes, an eluent guide tube is arranged on the other side of the first chamber, and a tumor cell outlet is formed in the top of the first chamber; a plurality of air blowing pipes communicated with the second chamber are arranged on one side of the second chamber, and an erythrocyte eduction pipe is arranged on one side of the second chamber; one side of the third chamber is provided with a cleaning liquid guide pipe, and one side of the bottom of the third chamber is provided with a effusion outlet.

2. The capturing device for circulating tumor cells according to claim 1, wherein: the end part of the inflation tube is connected with an extrusion air bag.

3. The capturing device for circulating tumor cells according to claim 1, wherein: the end part of the air blowing pipe is connected with an inflatable air bag.

4. The capturing device for circulating tumor cells according to claim 1, wherein: the filtration membrane layer comprises a first filtration membrane layer and a second filtration membrane layer, the first filtration membrane layer is located between the first chamber and the second chamber, and the second filtration membrane layer is located between the second chamber and the third chamber.

5. The capturing device for circulating tumor cells according to claim 1, wherein: the third chamber is funnel-shaped.

6. The capturing device for circulating tumor cells according to claim 4, wherein: the interception aperture of the first filtering membrane layer is 5-8 um.

7. The capturing device for circulating tumor cells according to claim 4, wherein: the interception aperture of the second filtering membrane layer is 2-4 um.