CN106834098A - A kind of device of the rapid extraction human stem cell for accurate medical treatment - Google Patents
A kind of device of the rapid extraction human stem cell for accurate medical treatment Download PDFInfo
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- CN106834098A CN106834098A CN201710147654.0A CN201710147654A CN106834098A CN 106834098 A CN106834098 A CN 106834098A CN 201710147654 A CN201710147654 A CN 201710147654A CN 106834098 A CN106834098 A CN 106834098A
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- diversion cavity
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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Abstract
The present invention relates to a kind of device of the rapid extraction human stem cell for accurate medical treatment, including bearing base, frame, knockout drum and recycling can, wherein frame is arranged on bearing base upper surface, knockout drum and recycling can are embedded in frame, knockout drum bottom is connected by mozzle with recycling can top, knockout drum includes sampling syringe needle, sampling pipe, protection tank body, separation screening chamber, negative pressure pump, ultrasonic oscillation device and cell filtration screen cloth, protection tank body includes supporting body and closure, separation screening chamber includes connecting hoop, diversion cavity and seal plug, cell filtration screen cloth is distributed in each diversion cavity and is coaxially distributed with diversion cavity, ultrasonic oscillation device is separately mounted to each diversion cavity side-wall outer side.The present invention can effectively improve the operating efficiency of stem cell separation and Extraction, the risk that cell sustains damage in an in vitro environment by exposure be reduced, it is ensured that the bioactivity of stem cell, the need for reaching clinical treatment and scientific research.
Description
Technical field
It is exactly a kind of rapid extraction people's soma for accurate medical treatment the present invention relates to a kind of medical auxiliary equipment
The device of cell.
Background technology
Now with stem cells technology research and the development of application technology, currently in numerous biological medicine research and disease
Clinical treatment activity in, stem cells technology is progressively promoted and used, but in real work discovery, currently carrying out
When stem cell extracts operation, although the need for current extraction equipment can be met to stem cell extraction operation, extraction equipment
Often complex operation, with high costs and relatively low to the operating efficiency that stem cell extracts operation, so that on the one hand serious restriction
The popularization and application of stem cells technology, cause the current stem cells technology often to limit in the lab, make in clinical treatment
With few, on the other hand also result in stem cell is extracted, it is more long due to expending the time, stem cell is increased exposed to outside
Time in environment, polluted and the risk of loss of biological activity by external environment condition so as to increased stem cell, therefore caused current
There is greatly puzzlement in stem cells technology, for this present situation, carried in the urgent need to developing a kind of stem cell of invention in the application
Take device, to meet actual work the need for.
The content of the invention
It is of the invention to provide a kind of thin for accurate medical rapid extraction people's soma for not enough present on prior art
The device of born of the same parents, the invention constitutes simple structure, flexible and convenient to use, easy to operate and use cost is cheap, can effectively improve
The operating efficiency of stem cell separation and Extraction, reduces the risk that cell sustains damage in an in vitro environment by exposure, it is ensured that dry thin
The bioactivity of born of the same parents, the need for reaching clinical treatment and scientific research.
To achieve these goals, the present invention is to realize by the following technical solutions:
A kind of device of rapid extraction human stem cell for accurate medical treatment, including bearing base, frame, knockout drum and recovery
Tank, wherein described frame be frame structure, be coaxially distributed installed in bearing base upper surface and with bearing base, knockout drum and
Recycling can is embedded in frame, and is connected with housing slide by slide rail, and knockout drum bottom is by mozzle and recycling can top
Connection, knockout drum includes sampling syringe needle, sampling pipe, protection tank body, separation screening chamber, negative pressure pump, ultrasonic oscillation device and thin
Born of the same parents' filter screen, protection tank body includes supporting body and closure, and closure is connected with each other and constitutes airtight cavity knot with supporting body
Structure, closure upper surface and supporting body lower surface are all provided with pod apertures, and the wherein pod apertures of closure upper surface are mutual with sampling pipe
Connection, the pod apertures of supporting body lower surface are connected with each other with mozzle, and sampling pipe front end is connected with syringe needle of sampling, end with separate
Screening chamber front end face is connected with each other, and separation screening chamber terminal surface is interconnected with negative pressure pump, and separation screening chamber is embedded in knockout drum
In supporting body, and coaxially it is distributed with supporting body, and is connected with each other by slide rail between separation screening chamber and supporting body, and retains 1-3
Millimeter external series gap, separation screening chamber includes connecting hoop, diversion cavity and seal plug, diversion cavity at least three and along carrier shaft
Line direction is uniform, is connected with each other by connecting hoop between diversion cavity, and positioned at diversion cavity and leading positioned at least significant end foremost
Seal plug is all provided with stream resonator end surface, wherein diversion cavity foremost is interconnected with sampling pipe, least significant end diversion cavity and negative pressure
Pump is interconnected, and cell filtration screen cloth is some, is distributed in each diversion cavity and is coaxially distributed with diversion cavity, and in each diversion cavity
It is all provided with least one cell filtration screen cloth, cell filtering net mesh is from being located at diversion cavity foremost position to diversion cavity position foremost
Put and successively decrease step by step, ultrasonic oscillation device is some, and be separately mounted to each diversion cavity side-wall outer side, and each ultrasonic oscillation device
It is distributed in the shape of a spiral around water conservancy diversion cavity axis.
Further, described protection tank inner surface separately sets semiconductor cooling device and electric heater unit, and semiconductor
Spaced distribution between refrigerating plant and electric heater unit.
Further, irradiation inactivated device, and described irradiation inactivated dress are set between described separation screening chamber and supporting body
Put on protection tank inner surface.
Further, separately it is connected with each other by some elastic cushion blocks between described separation screening chamber and supporting body.
Further, when the cell filtration screen cloth in described diversion cavity is two or more, then same diversion cavity
Cell filtration screen cloth mesh diameter it is identical.
Further, in described diversion cavity, elastic seal ring is set between the contact surface of adjacent two diversion cavities, and it is described
Elastic seal ring is coated on diversion cavity outer surface.
The present invention constitutes simple structure, flexible and convenient to use, easy to operate and use cost is cheap, can effectively improve dry
The operating efficiency that cell separation is extracted, reduces the risk that cell sustains damage in an in vitro environment by exposure, it is ensured that stem cell
Bioactivity, the need for reaching clinical treatment and scientific research.
Brief description of the drawings
Describe the present invention in detail with reference to the accompanying drawings and detailed description;
Fig. 1 is schematic structural view of the invention.
Specific embodiment
For technological means, creation characteristic, reached purpose and effect for making present invention realization are easy to understand, with reference to specific
Implementation method, is expanded on further the present invention.
A kind of device of rapid extraction human stem cell for accurate medical treatment as described in Figure 1, including bearing base 1,
Frame 2, knockout drum 3 and recycling can 4, wherein frame 2 be frame structure, installed in the upper surface of bearing base 1 and with bearing base 1
Coaxial distribution, knockout drum 3 and recycling can 4 are embedded in frame 2, and are slidably connected with frame 2 by slide rail 5, the bottom of knockout drum 3
Connected with the top of recycling can 4 by mozzle 6, knockout drum 3 includes sampling needle 31, sampling pipe 32, protection tank body 33, bolter
Select chamber 34, negative pressure pump 35, ultrasonic oscillation device 36 and cell filtration screen cloth 37, protection tank body 33 includes supporting body 331 and close
Capping 332, closure 332 and supporting body 331 are connected with each other and constitute airtight cavity structure, the upper surface of closure 332 and carrying
The lower surface of body 331 is all provided with pod apertures 333, and the wherein pod apertures 333 of the upper surface of closure 332 are connected with each other with sampling pipe 32, hold
The pod apertures 333 of the lower surface of carrier 331 are connected with each other with mozzle 6, the front end of sampling pipe 32 with sampling syringe needle 31 be connected, end with
The front end face of separation screening chamber 34 is connected with each other, and the terminal surface of separation screening chamber 34 is interconnected with negative pressure pump 35, separation screening chamber 34
Be embedded in the supporting body 331 of knockout drum 33, and with the coaxial distribution of supporting body 331, and between separation screening chamber 34 and supporting body 331 lead to
Slide rail 5 is crossed to be connected with each other, and retain 1-3 millimeters of external series gaps 6, separation screening chamber 34 include connecting hoop 341, diversion cavity 342 and
Seal plug 343, diversion cavity 342 at least three is simultaneously uniform along the axis direction of supporting body 331, and connecting hoop is passed through between diversion cavity 342
341 are connected with each other, and are all provided with seal plug positioned at diversion cavity 342 and positioned at least significant end the end of diversion cavity 342 foremost
First 343, wherein diversion cavity 342 foremost is interconnected with sampling pipe 32, least significant end diversion cavity 342 is mutually interconnected with negative pressure pump 35
Logical, cell filtration screen cloth 37 is some, be distributed in each diversion cavity 342 and with the coaxial distribution of diversion cavity 342, and each diversion cavity
It is all provided with least one cell filtration screen cloth 37 in 342, the mesh of cell filtering net 37 is from being located at the foremost position of diversion cavity 342 to leading
The stream foremost position of chamber 342 is successively decreased step by step, and ultrasonic oscillation device 36 is some, and is separately mounted to outside the side wall of each diversion cavity 342
Side, and each ultrasonic oscillation device 36 is distributed around the axis of diversion cavity 342 in the shape of a spiral.
In the present embodiment, the described inner surface of protection tank body 33 separately sets semiconductor cooling device 7 and electric heater unit 8, and
Spaced distribution between semiconductor cooling device 7 and electric heater unit 8.
In the present embodiment, irradiation inactivated device 9, and described spoke are set between described separation screening chamber 34 and supporting body 331
It is arranged on the protection inner surface of tank body 33 according to inactivating device 9.
In the present embodiment, separately mutually interconnected by some elastic cushion blocks 10 between described separation screening chamber 34 and supporting body 331
Connect.
It is when the cell filtration screen cloth 37 in described diversion cavity 342 is two or more, then same in the present embodiment
The mesh diameter of cell filtration screen cloth 37 of diversion cavity 342 is identical.
In the present embodiment, in described diversion cavity 342, elastic seal ring is set between the contact surface of adjacent two diversion cavities 342
11, and described elastic seal ring 11 is coated on the outer surface of diversion cavity 342.
In the specific implementation, be installed in place for bearing base, frame, knockout drum and recycling can first by the present invention, and will divide
Connected between tank and recycling can, then sampling syringe needle is connected with human body, and start negative pressure pump simultaneously so that in human body
The body fluid such as blood are entered into separation screening chamber by syringe needle of sampling under suction function along sampling pipe, then in the negative of negative pressure pump
Under pressure effect, the body fluid for collecting passes sequentially through each cell filtration screen cloth and carries out different cell screenings separation along mozzle, and
Being driven by negative pressure in body fluid carries out cell screening separation simultaneously, and separately carrying out concussion to body fluid by ultrasonic oscillation device stirs
Mix, reach the purpose for improving detached job efficiency, by separating, the cell such as stem cell is fixed point by each cell filtration screen cloth
From temporarily the body fluid after separation is then entered into recycling can and preserved, it is to avoid surrounding enviroment are polluted.
Detached job is extracted stem cell is carried out simultaneously, can separately be added by the semiconductor cooling device and electricity that protect on tank body
Environment temperature when thermal is to detached job is adjusted, and is easy to maintain stem cell biological activity, while can also be by irradiation
Inactivating device carries out overall sterilization process to knockout drum, it is to avoid biological factor causes Stem cells injury.
The present invention constitutes simple structure, flexible and convenient to use, easy to operate and use cost is cheap, can effectively improve dry
The operating efficiency that cell separation is extracted, reduces the risk that cell sustains damage in an in vitro environment by exposure, it is ensured that stem cell
Bioactivity, the need for reaching clinical treatment and scientific research
General principle of the invention and principal character and advantages of the present invention has been shown and described above.The technical staff of the industry
It should be appreciated that the present invention is not limited to the above embodiments, the present invention is simply illustrated described in above-described embodiment and specification
Principle, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these change and
Improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appending claims and its equivalent
Thing is defined.
Claims (6)
1. the device of a kind of rapid extraction human stem cell for accurate medical treatment, it is characterised in that:It is described for precisely medical treatment
The quick device for carrying human stem cell include bearing base, frame, knockout drum and recycling can, wherein described frame is framework
Structure, is coaxially distributed installed in bearing base upper surface and with bearing base, and described knockout drum and recycling can is embedded in frame
It is interior, and be connected with housing slide by slide rail, described knockout drum bottom is connected by mozzle with recycling can top, described point
Include sampling syringe needle, sampling pipe, protection tank body, separation screening chamber, negative pressure pump, ultrasonic oscillation device and cell filtration sieve from tank
Net, described protection tank body includes supporting body and closure, and described closure is connected with each other and constitutes closed chamber with supporting body
Body structure, described closure upper surface and supporting body lower surface is all provided with pod apertures, wherein the pod apertures of closure upper surface with
Sampling pipe is connected with each other, pod apertures and mozzle interconnection, described sampling pipe front end and the sampling needle of supporting body lower surface
Head connection, end is connected with each other with separation screening chamber front end face, and described separation screening chamber terminal surface is interconnected with negative pressure pump,
Described separation screening chamber is embedded in the supporting body of knockout drum, and is coaxially distributed with supporting body, and separation screening chamber and supporting body
Between be connected with each other by slide rail, and retain 1-3 millimeters of external series gaps, described separation screening chamber include connecting hoop, diversion cavity and
Seal plug, described diversion cavity at least three is simultaneously uniform along supporting body axis direction, and connecting hoop is passed through between described diversion cavity
It is connected with each other, and seal plug is all provided with positioned at diversion cavity and positioned at least significant end diversion cavity end foremost, wherein institute
The diversion cavity foremost stated is interconnected with sampling pipe, and least significant end diversion cavity is interconnected with negative pressure pump, described cell mistake
Screen net is some, is distributed in each diversion cavity and is coaxially distributed with diversion cavity, and at least one cell is all provided with each diversion cavity
Filter screen, described cell filtering net mesh is passed step by step positioned at diversion cavity foremost position to diversion cavity foremost position certainly
Subtract, described ultrasonic oscillation device is some, and be separately mounted to each diversion cavity side-wall outer side, and each ultrasonic oscillation device is equal
It is distributed in the shape of a spiral around water conservancy diversion cavity axis.
2. a kind of device of rapid extraction human stem cell for accurate medical treatment according to claim 1, its feature exists
In described protection tank inner surface separately sets semiconductor cooling device and electric heater unit, and semiconductor cooling device and electricity add
Spaced distribution between thermal.
3. a kind of device of rapid extraction human stem cell for accurate medical treatment according to claim 1, its feature exists
In, irradiation inactivated device is set between described separation screening chamber and supporting body, and described irradiation inactivated device is arranged on safety can
On internal surface.
4. a kind of device of rapid extraction human stem cell for accurate medical treatment according to claim 1, its feature exists
In being separately connected with each other by some elastic cushion blocks between described separation screening chamber and supporting body.
5. a kind of device of rapid extraction human stem cell for accurate medical treatment according to claim 1, its feature exists
In, when cell filtration screen cloth in described diversion cavity is two or more, then the cell filtration screen cloth of same diversion cavity
Mesh diameter is identical.
6. a kind of device of rapid extraction human stem cell for accurate medical treatment according to claim 1, its feature exists
In in described diversion cavity, elastic seal ring, and described elastic seal ring cladding being set between the contact surface of adjacent two diversion cavities
In diversion cavity outer surface.
Priority Applications (1)
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CN201710147654.0A CN106834098B (en) | 2017-03-13 | 2017-03-13 | A kind of device of the rapidly extracting human stem cell for accurate medical treatment |
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CN201710147654.0A CN106834098B (en) | 2017-03-13 | 2017-03-13 | A kind of device of the rapidly extracting human stem cell for accurate medical treatment |
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CN106834098A true CN106834098A (en) | 2017-06-13 |
CN106834098B CN106834098B (en) | 2019-05-24 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112063502A (en) * | 2020-09-14 | 2020-12-11 | 于爱玲 | Biological culture device based on hemodialyzer |
Citations (4)
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CN102174395A (en) * | 2011-01-30 | 2011-09-07 | 中国科学院广州生物医药与健康研究院 | Automation augmentation and culture system of induced pluripotent stem cells |
CN103614339A (en) * | 2013-11-22 | 2014-03-05 | 武汉友芝友医疗科技有限公司 | Cell separation method |
US20150328634A1 (en) * | 2012-11-07 | 2015-11-19 | Life Technologies Corporation | Case for containing biological samples and corresponding method of use |
CN205613146U (en) * | 2016-05-16 | 2016-10-05 | 新疆维吾尔自治区人民医院 | Filter equipment a little shakes suitable for marrow filters |
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2017
- 2017-03-13 CN CN201710147654.0A patent/CN106834098B/en active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102174395A (en) * | 2011-01-30 | 2011-09-07 | 中国科学院广州生物医药与健康研究院 | Automation augmentation and culture system of induced pluripotent stem cells |
US20150328634A1 (en) * | 2012-11-07 | 2015-11-19 | Life Technologies Corporation | Case for containing biological samples and corresponding method of use |
CN103614339A (en) * | 2013-11-22 | 2014-03-05 | 武汉友芝友医疗科技有限公司 | Cell separation method |
CN205613146U (en) * | 2016-05-16 | 2016-10-05 | 新疆维吾尔自治区人民医院 | Filter equipment a little shakes suitable for marrow filters |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112063502A (en) * | 2020-09-14 | 2020-12-11 | 于爱玲 | Biological culture device based on hemodialyzer |
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Effective date of registration: 20190425 Address after: Room 1221, No. 31 Lane, No. 2, Jiading District, Shanghai 201800 Applicant after: Shanghai whitening Biotechnology Co., Ltd. Address before: 610041 No. 1, 8 floor, No. 1, Tianfu Avenue, 1388 middle and high tech Zone, Chengdu, Sichuan. Applicant before: CHENGDU YUYA SCIENCE & TECHNOLOGY CO., LTD. |
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