CN210200665U

CN210200665U - Full-automatic single cell mass spectrum detection sample injector

Info

Publication number: CN210200665U
Application number: CN201921046653.8U
Authority: CN
Inventors: Ting Xu; 徐艇
Original assignee: Wuxi Ruisheng Medical Technology Co ltd
Current assignee: Wuxi Ruisheng Medical Technology Co ltd
Priority date: 2019-05-10
Filing date: 2019-07-06
Publication date: 2020-03-27
Anticipated expiration: 2029-07-06

Abstract

The utility model discloses a full-automatic unicellular mass spectrometry injector, including the mount, the mount is provided with the extraction system on, is provided with hollow nanometer probe on the extraction system, and the lower extreme of mount is provided with the workstation, and hollow nanometer probe below is unicellular sample, is provided with fluorescence spectrum collection analytic system on the workstation of unicellular sample below mount, is provided with the fluorescence microscope of inversion on the hollow nanometer probe, and the injector is still connected to hollow nanometer probe. The utility model discloses an extract single intracellular material, like cytoplasm, cell nucleus etc. then send the mass spectrometer injector automatically to carry out the composition analysis. Automatic sampling, conveying sample to the mass spectrum introduction port, the operating time that can significantly reduce guarantees sample quality, improves detection effect.

Description

Full-automatic single cell mass spectrum detection sample injector

Technical Field

The utility model relates to a cell mass spectrum detects injector belongs to biological detection technical field.

Background

At present, single cell mass spectrometry can only be manually sampled on a microscope and then sent to a sample inlet of a mass spectrometer, has the defects of complex operation, long operation time, easy damage to a sampling needle and the like, and is particularly not suitable for sample introduction of volatile samples.

Disclosure of Invention

The utility model aims to solve the technical problem that a safe, reliable full-automatic unicellular mass spectrometry detection injector is provided.

The technical scheme of the utility model is that:

the utility model provides a full-automatic unicellular mass spectrometry injector, includes the mount, is provided with extraction system on the mount, is provided with hollow nanometer probe on the extraction system, and the lower extreme of mount is provided with the workstation, is the unicellular sample on the workstation of hollow nanometer probe below, and unicellular sample below is provided with the fluorescence microscope of inversion on the workstation, and hollow nanometer probe still connects the injector.

The utility model has the advantages that:

extraction of single intracellular material: the hollow nanometer needle (the minimum diameter is30 nm) is accurately positioned inside the cell, such as the positions of cytoplasm, nucleus and the like, through a fixing frame (the precision is 200 nm), then the material extraction is carried out through an extraction system, and other products can not achieve the function;

automatic transfer of the extracted micro-samples: the extraction system is provided with an automatic transmission arm, can extend in the horizontal direction and rotate by 90 degrees, and is sent to a sample inlet of a mass spectrometer for component analysis, and the sample introduction time of a single sample is about 30 seconds;

the compatibility is good: the automatic sampling system adopts an independent movable fixed system, and simultaneously, the adjustable distance and the height range of the sampling needle are wider, so that the automatic sampling system can be used with various mass spectrometers, has excellent compatibility, and reduces the use cost of customers;

the utility model discloses an extract single intracellular material, like cytoplasm, cell nucleus etc. then send the mass spectrograph introduction port automatically and carry out the composition analysis. The automatic sampling and the sample transmission to the mass spectrum sample injector can greatly reduce the operation time, ensure the sample quality and improve the detection effect.

Drawings

Fig. 1 is a schematic structural diagram of the present invention;

fig. 2 is a partially enlarged mass spectrometry spectrum in an embodiment of the invention;

fig. 3 is an overall detection mass spectrum in an embodiment of the present invention.

In the figure: 1-fixed frame, 2-hollow nanometer probe, 3-single cell sample, 4-sample injector, 5-extraction system, 6-inverted fluorescence microscope.

Detailed Description

The invention is further described with reference to the following specific drawings and examples.

The utility model provides a full-automatic unicellular mass spectrometry injector, includes mount 1, is provided with extraction system 5 on the mount 1, is provided with hollow nanometer probe 2 on the extraction system 5, and the lower extreme of mount 1 is provided with the workstation, is unicellular sample 3 on the workstation of hollow nanometer probe 2 below, and unicellular sample 3 below is provided with inverted fluorescence microscope 6 on the workstation, and hollow nanometer probe 2 still connects injector 4.

During work, the extraction system 5 drives the hollow nanoprobe 2 to move up and down on the upright post of the fixing frame 1, the up-down distance between the hollow nanoprobe 2 and the single cell sample 3 is adjusted, and the front-back, left-right positions can be adjusted at the same time, after the hollow nanoprobe 2 sucks the single cell sample 3 into the pinhole, the single cell sample is rotationally transferred into the sample injector 4 for component analysis. The single intracellular material may be the cytoplasm or the nucleus. The automatic sampling and the sample transmission to the mass spectrum sample injector 4 can greatly reduce the operation time, ensure the sample quality and improve the detection effect.

The fixing frame 1 is manufactured by Jiangsu Ruiming biological company, and has the following model: GD 02; the hollow nanometer needle 2 is produced by Jiangsu Ruiming biological company, and has the following model: RMA-P-H; the single-cell sample 3 was obtained by (product No. CBP60232 manufactured by Cobioer Co., Ltd.; sample injector 4 manufactured by Riming Bio Inc. of Jiangsu, model No. YTQ 300; extraction System 5 manufactured by Riming Bio Inc. of Jiangsu, model No. FIS 300; and inverted fluorescence microscope 6 manufactured by Orinbas, model No. CKX 31.

Example (b):

experimental materials: the sample was HeLa cells (human cervical cancer cells, manufactured by Cobioer Co., Ltd., cat # CBP 60232),

fixing frame 1: minimum adjustment accuracy 200nm, left-right adjustment distance range: 1mm-500mm, the range of the up-down adjusting distance: 20mm-15000mm, angle adjusting range: 15-120 degrees; hollow nanoneedle 2: a minimum diameter of 30 nm; sample injector 4: the inner diameter of the sample inlet is 1 mm; the extraction system 5: minimum extraction volume 15 fL; inverted fluorescence microscope 6: the online fluorescence imaging function can be realized, and the minimum optical resolution is 1 um; mass spectrometry: product of TransMIT, model number, germany: AP-SMALDI 10

The experimental process comprises the following steps:

the sample cells 3 are attached to the bottom of the culture dish (before the experiment, the culture dish is filled with phosphate buffered saline-PBS and is placed in a carbon dioxide incubator for 10 hours at 20 ℃), and the culture dish is positioned in the middle of the stage of the inverted fluorescence microscope; moving the hollow nano needle 2 to the top position of the cell through the transverse axis (X axis) and the longitudinal axis (Z axis) of the fixing frame 1; adjusting the Z axis of the fixing frame to insert the hollow nanometer needle 2 into the cell to a depth of about 100nm (the depth can be controlled by micromanipulation); to the area of the substance to be extracted; starting an extraction system 5, adjusting the extraction working voltage to 3V, adjusting the extraction time to 5 seconds, and starting extraction; after extraction, the probe is moved upwards (Z axis) by 300mm by the fixing frame, then rotated 90 degrees in the anticlockwise direction, and finally moved 450mm in the horizontal direction, and the probe tip of the hollow probe enters the sample port of the sample injector by 200 mm; the sample injector starts to work, and the trace substances in the hollow needle are sucked out through the vacuum interface and are conveyed into a sample cavity of the mass spectrometer; the mass spectrometer starts the sample analysis.

The experimental results are as follows:

through mass spectrometry, a plurality of substance components in the cells can be clearly resolved, wherein FIG. 3 is a whole detection map (0-280), FIG. 2 is a partial amplification map (75.7-154), and six substance components are respectively marked by I-VI in FIG. 2.

Claims

1. The utility model provides a full-automatic unicellular mass spectrometry injector, includes mount (1), its characterized in that: the device is characterized in that an extraction system (5) is arranged on the fixing frame (1), a hollow nano probe (2) is arranged on the extraction system (5), a workbench is arranged at the lower end of the fixing frame (1), a single-cell sample (3) is arranged on the workbench below the hollow nano probe (2), an inverted fluorescence microscope (6) is arranged below the single-cell sample (3) on the workbench, and the hollow nano probe (2) is further connected with a sample injector (4).