CN110736639A - Single cell automatic sampling instrument - Google Patents
Single cell automatic sampling instrument Download PDFInfo
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- CN110736639A CN110736639A CN201911217554.6A CN201911217554A CN110736639A CN 110736639 A CN110736639 A CN 110736639A CN 201911217554 A CN201911217554 A CN 201911217554A CN 110736639 A CN110736639 A CN 110736639A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/08—Devices for withdrawing samples in the solid state, e.g. by cutting involving an extracting tool, e.g. core bit
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- Immunology (AREA)
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- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses an single cell automatic sampling instrument, which comprises a positive imaging system, a micro-cutting system, a sample stage system and a sample extraction system, wherein the positive imaging system comprises a high-definition imaging CCD and a display which are arranged on a CCD adjusting bracket, the micro-cutting system comprises a hollow cutting needle head, a cutting probe adjusting bracket and a high-speed motor connected with the hollow cutting needle head, the hollow cutting needle head and the high-speed motor are arranged on the cutting probe adjusting bracket, the sample stage system comprises a sample stage and a sample heating platform arranged on the sample stage, a sample sheet is arranged on the sample heating platform, the hollow cutting needle head is aligned to the position right above a region to be cut of the sample sheet through the cutting probe adjusting bracket, the region to be cut is imaged on the display through the CCD adjusting bracket, the sample extraction system comprises a suction pipe connected with the hollow cutting needle head and a vacuum pump connected with the suction pipe, and a graduated scale is arranged on the sample heating platform.
Description
Technical Field
The invention relates to an single cell automatic sampling instrument, belonging to the application of precision automation and image recognition technology in the technical field of biological detection.
Background
The single cell separation belongs to the important technology of sample pretreatment of single cell research, and the direct acquisition of single cell or micro-area samples on tissues or living bodies is more close to the actual living environment of cells, and has positive significance in the fields of clinical, biology and pharmacy.
Disclosure of Invention
The invention aims to solve the technical problem of providing single cell or micro-area biological sample extractors which are rapid, have extremely low influence on the biological activity of samples and have moderate cost.
The technical scheme of the invention is as follows:
automatic sampling appearance of unicellular, including just putting imaging system, the microdissection system, sample platform system and sample extraction system, just putting imaging system including setting up high definition formation of image CCD and the display on CCD adjusts the support, the microdissection system includes hollow cutting syringe needle, the cutting probe alignment jig, the high-speed motor who is connected with hollow cutting syringe needle, hollow cutting syringe needle and high-speed motor set up on the cutting probe alignment jig, sample platform system includes sample platform and the sample warm table of setting on sample platform, set up the sample piece on the sample warm table, hollow cutting syringe needle aligns the sample piece through the cutting probe alignment jig and treats directly over the cutting region, adjust high definition formation of image CCD through CCD and will treat the region of cutting on the display, the article extraction system includes the straw of being connected with hollow cutting syringe needle, and the vacuum pump of being connected with the straw.
And a graduated scale is arranged on the sample heating table.
The invention has the beneficial effects that:
accurate positioning of the micro-area image: during sample pretreatment, a mark is made on a region to be cut, and the upright imaging system can perform accurate positioning and provide an accurate coordinate position for the cutting probe.
Accurate positioning of a micro-area of a cutting probe: the hollow cutting probe precisely moves to a micro area to be cut positioned by the upright imaging system through the cutting probe adjusting frame.
Micron-level hollow cutting probe: the hollow cutting probe is made of a novel composite material and prepared by a micro-nano semiconductor processing technology, has the technical advantages of micron size, good flexibility, difficulty in breaking and the like, and has the characteristics of small damage, controllable cutting position and size, simplicity in operation, low cost and the like by mechanically cutting a sample through the microneedle.
And image recognition and micro-area cutting are linked, namely after the image is recognized to the area to be cut, a signal is fed back to the cutting system, the motor and the cutting probe adjusting frame start to automatically align to the image recognition area, and cutting is started after the distance is set, so that the responsiveness and accuracy are ensured.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a top view of the overall profile of the sample after cutting;
figure 3 is a top view of the heating plate with scale markings.
In the figure: 1-high-definition imaging CCD, 2-display, 3-CCD adjusting bracket, 4-hollow cutting probe, 5-motor, 6-cutting probe adjusting bracket, 7-sample table, 8-sample heating table, 9-suction pipe, 10-vacuum pump, 11-micro-area cut sample, 12-sample slice, 13-micro area to be cut of sample.
Detailed Description
The invention is further illustrated in the following detailed description of the invention and examples.
automatic sampling appearance of unicellular, including just putting imaging system, the microdissection system, sample platform system and sample extraction system, just putting imaging system including setting up high definition formation of image CCD1 and display 2 on CCD adjusts support 3, the microdissection system includes hollow cutting syringe needle 4, cutting probe alignment jig 6, high-speed motor 5 connected with hollow cutting syringe needle 4, hollow cutting syringe needle 4 and high-speed motor 5 set up on cutting probe alignment jig 6, the sample platform system includes sample platform 7 and the sample warm table 8 of setting on sample platform 7, set up sample piece 12 on the sample warm table 8, hollow cutting syringe needle 4 aims at sample piece 12 through cutting probe alignment jig 6 and treats directly over the cutting region, adjust high definition formation of image CCD1 through CCD alignment jig 3 and will treat the region of cutting and image on display 2, the sample extraction system includes straw 9 connected with hollow cutting syringe needle 4, and the vacuum pump 10 connected with straw 9.
A graduated scale is arranged on the sample heating table 8.
The method comprises the steps of placing a sample piece 12 on a sample table 7, starting a heating device 8 if heating is needed, heating at 20-80 degrees, enabling the upper surface to be provided with scale marks to facilitate sample placement, adjusting the focal length of a high-definition imaging CCD1 by adjusting the position of a CCD adjusting bracket 33, enabling the high-definition imaging CCD1 to move back and forth and rotate at 360 degrees, enabling a micro area 13 (a common range: 100-2500 um area, and marking other small areas with cutting according to actual requirements) to be clearly imaged, enabling images to be directly observed through a display 2, adjusting a hollow cutting probe 4 and a cutting probe adjusting bracket 6, namely adjusting the cutting probe adjusting bracket 6 to move back and forth and left and right, enabling the hollow cutting probe 4 to be perpendicular to the surface of the sample piece 12, adjusting the hollow cutting probe 4 to be right above the area to be cut through the cutting probe adjusting bracket 6, setting the height coordinate of the hollow cutting probe 4 to be 0, setting the cutting depth, setting the cutting probe rotating speed to be equal to the cutting probe 4, setting the cutting probe 4 to be equal to the cutting speed ( is set on the sample piece, increasing the thickness of the sample piece 4, starting a vacuum pump to be set, and enabling the cutting probe 4 to be set after the cutting probe 4 and the cutting probe 4 to be set, the cutting probe 4, the cutting probe is set to be set, and the cutting probe.
Example (b):
experimental materials: the sample is a mouse fresh brain slice (thickness of about 45um, size 15' 12 mm);
CCD1 magnification: 40 times of the total weight;
a motor 5: light noise, small vibration and 120rpm of minimum rotating speed;
moving the CCD adjusting bracket 3 and the cutting probe adjusting bracket 6: minimum precision 10 um;
hollow cutting probe 4: the inner diameter is 1000um, and the outer diameter is 1500 um;
high-definition imaging CCD 1: the online imaging function can be realized;
sample heating stage 8: the temperature was 25 degrees celsius and the sample was placed in the middle position.
The experimental process comprises the following steps:
the tissue sample slice is laid at the center of the sample heating table 8, and the position to be cut is marked under the guidance of the high-definition imaging CCD 1;
position adjustment: precisely adjusting the hollow cutting probe 4 to the marked area by moving the CCD adjusting bracket 3 and the cutting probe adjusting bracket 6, and then adjusting the Z axis in the cutting probe adjusting bracket 6 to enable the hollow cutting probe 4 to be in contact with the upper surface of the brain slice of the mouse;
setting parameters: the height coordinate of the probe is set to be 0, and the cutting depth is 47um, the cutting rotating speed is 125rpm, and the falling speed of the probe is 50 um/min;
cutting: after the setting is finished, starting the motor 5 and the cutting probe adjusting frame 6, and rotating the hollow cutting probe 4 to start cutting;
sample treatment: after 1 minute, the micro-area cut sample 11 will remain in the hollow cutting probe 4 after cutting, and the sample is blown out by positive pressure provided by the vacuum pump 10 through the suction pipe 9 of the sample extraction system and placed in the sample storage position.
1-high-definition imaging CCD, 2-display, 3-CCD adjusting bracket, 4-hollow cutting probe, 5-motor, 6-cutting probe adjusting bracket, 7-sample table, 8-sample heating table, 9-suction pipe, 10-vacuum pump, 11-micro-area cut sample, 12-sample slice, 13-micro area to be cut of sample.
The high-definition imaging CCD1 is produced by Shenzhen ANDONSTAR company, and has the model number: a1; the display 2 is produced by Shenzhen profit corporation, model number HDV 20; the CCD adjusting bracket 3 is produced by Jiangsu Ruiming biology, and has the following model: WCJ100, respectively; the hollow cutting probe 4 is produced by Jiangsu Ruiming biology, and has the following model: MPC 300; the motor 5 is manufactured by Shanghai Whitledo electromechanical Co., Ltd., model number: 775A; the cutting probe adjusting frame 6 is produced by Jiangsu Ruiming biology, and has the following model: MPC 500; the sample table 7 is produced by Jiangsu Ruiming biology, and has the following model: SP 100); the sample heating stage 8 is manufactured by ge danna instruments ltd, model: HT-200; the straw 9 is manufactured by Beijing Oriental essence park science and technology Limited, model: SGK-2 LB-T; the vacuum pump 10 is manufactured by Beijing Oriental essence park science and technology Limited, model: SGK-2 LB.
Claims (2)
- The automatic sampling instrument for the single cells comprises a positive imaging system, a microdissection system, a sample stage system and a sample extraction system, and is characterized in that the positive imaging system comprises a high-definition imaging CCD (1) and a display (2) which are arranged on a CCD adjusting bracket (3), the microdissection system comprises a hollow cutting needle head (4), a cutting probe adjusting bracket (6), a high-speed motor (5) connected with the hollow cutting needle head (4), the hollow cutting needle head (4) and the high-speed motor (5) are arranged on the cutting probe adjusting bracket (6), the sample stage system comprises a sample stage (7) and a sample heating stage (8) arranged on the sample stage (7), a sample sheet (12) is arranged on the sample heating stage (8), the hollow cutting needle head (4) is aligned to a position right above a region to be cut of the sample sheet (12) through the cutting probe adjusting bracket (6), the high-definition imaging CCD to be cut region is imaged on the display (2) through the CCD adjusting bracket (3), and the sample extraction system comprises a suction pipe (9) connected with the hollow cutting probe adjusting bracket (4) and a vacuum pump (10).
- 2. The automatic single cell sampler as claimed in claim 1, wherein a graduated scale is provided on the sample heating stage (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911217554.6A CN110736639A (en) | 2019-12-03 | 2019-12-03 | Single cell automatic sampling instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911217554.6A CN110736639A (en) | 2019-12-03 | 2019-12-03 | Single cell automatic sampling instrument |
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| Publication Number | Publication Date |
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| CN110736639A true CN110736639A (en) | 2020-01-31 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201911217554.6A Pending CN110736639A (en) | 2019-12-03 | 2019-12-03 | Single cell automatic sampling instrument |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111676279A (en) * | 2020-05-20 | 2020-09-18 | 东南大学 | Spatial transcription set construction method |
| CN113155510A (en) * | 2021-04-16 | 2021-07-23 | 伊达生物有限公司 | Tissue cell segmentation sampling system and method |
-
2019
- 2019-12-03 CN CN201911217554.6A patent/CN110736639A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111676279A (en) * | 2020-05-20 | 2020-09-18 | 东南大学 | Spatial transcription set construction method |
| CN113155510A (en) * | 2021-04-16 | 2021-07-23 | 伊达生物有限公司 | Tissue cell segmentation sampling system and method |
| CN113155510B (en) * | 2021-04-16 | 2024-05-10 | 伊达生物有限公司 | Tissue cell segmentation sampling system and method |
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