CN113174320B - Microorganism sampling instrument and method - Google Patents

Microorganism sampling instrument and method Download PDF

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Publication number
CN113174320B
CN113174320B CN202110393711.XA CN202110393711A CN113174320B CN 113174320 B CN113174320 B CN 113174320B CN 202110393711 A CN202110393711 A CN 202110393711A CN 113174320 B CN113174320 B CN 113174320B
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sampling
test tube
clamping
moving unit
transverse
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CN113174320A (en
Inventor
夏荣钧
杨运波
孔繁勇
唐婧媛
沈悦
栾姝
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Eastern Liaoning University
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Eastern Liaoning University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/08Flask, bottle or test tube
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/50Means for positioning or orientating the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/02Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention provides a microorganism sampling instrument and a method, wherein the microorganism sampling instrument comprises an operation table, a frame, a vertical moving unit, a horizontal moving unit and a turnover structure; the operation table is provided with a test tube placement area and a sampling area; through the arrangement of the structure, the clamping structure can move in the transverse direction, and then the position between the test tube storage area and the area to be sampled can be moved; in addition, can carry out the ascending position movement of vertical direction through vertical mobile unit, and then realize the vertical motion to the clamping structure through the vertical motion to the clamping structure, and then realize the sample test tube decline sample wait to detect the sample or descend the purpose of placing the test-tube rack. The setting through flip structure can realize the adjustment to the angle of putting of sample test tube in vertical orientation. The structure realizes the automatic sampling process of the sample, avoids the process of manually sampling, and is particularly suitable for large-batch and continuous operation.

Description

Microorganism sampling instrument and method
Technical Field
The invention relates to the field of microorganism sampling, in particular to a sampling operation before detection of microorganisms in the fields of medicine and the like.
Background
Detection of microorganisms in medicine is a very important technical means based on the theoretical and experimental methods of microbiology to achieve studies on the type, concentration and nature of microorganisms in medical samples, thereby determining the type and concentration of microorganisms in these medical samples analyzed to evaluate the health of the human body or the type of disease.
In addition, in modern medicine, microbial detection of acquired medical specimens has become an essential and important component of standardized, high-quality development control of modern medicine.
However, in the sampling of medical samples, a wide variety of sample types are encountered, including, for example, fluids, semi-solids, and different sampling means may be employed for these different types of medical samples. For medical samples in semi-solid or solid form, such as sludge encountered in medicine, body tissue, body cuttings, medical cultures, etc., it is often necessary for these semi-solid or solid form medical substances to be held by an experimenter and then to perform a scooping operation on the sample by means of a small scoop, then to re-place the sample on the small scoop into the sampling tube and then to place the sampling tube on a sampling tube rack for subsequent further operational detection. The operation flow is very complex, the working efficiency is low, the long-time labor of the experimenters is challenged, and the burden is further caused to the experimenters when a large number of continuous samples are sampled.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a device structure and a method for reducing the sampling burden of experimenters and realizing efficient and rapid sampling operation so as to facilitate later-stage microorganism detection.
The invention provides a microorganism sampling instrument which comprises an operating platform, a frame, a vertical moving unit, a horizontal moving unit and a turnover structure, wherein the operating platform is arranged on the frame;
the operation table is provided with a test tube placing area and a sampling area, the test tube placing area is used for placing a fixing frame for storing test tubes, and the sampling area is used for placing an object to be sampled;
the stand is detachably fixed on the operating platform;
the vertical moving unit is arranged on the frame;
the transverse moving unit is connected to the vertical moving unit, and the vertical moving unit drives the transverse moving unit to move in the vertical direction;
the overturning structure is connected to the transverse moving unit, and the transverse moving unit can drive the overturning structure to move in the transverse direction;
the clamping structure is arranged on the overturning structure and is used for clamping the sampling test tube.
The beneficial effect of above-mentioned scheme is: through the arrangement of the structure, the clamping structure can move in the transverse direction, and then the position between the test tube storage area and the area to be sampled can be moved; in addition, can carry out the ascending position movement of vertical direction through vertical mobile unit, and then realize the vertical motion to the clamping structure through the vertical motion to the clamping structure, and then realize the sample test tube decline sample wait to detect the sample or descend the purpose of placing the test-tube rack. The setting through flip structure can realize the adjustment to the angle of putting of sample test tube in vertical orientation. The structure realizes the automatic sampling process of the sample, avoids the process of manually sampling the medical sample, and is particularly suitable for large-batch and continuous operation.
The vertical moving unit is provided with a supporting plate, a driving screw rod, a sliding rod and a driving block, wherein the supporting plate is provided with a lifting notch, the driving screw rod and the sliding rod are arranged in parallel and are arranged in the lifting notch, the driving block is sleeved on the driving screw rod and the sliding rod, and is connected with the transverse moving unit, and the transverse moving unit moves in the vertical direction through the rotation of the driving screw rod.
The transverse moving unit is provided with a transverse plate, a transverse screw rod, a sliding rod and moving blocks, wherein the transverse screw rod and the sliding rod are arranged in parallel and are arranged in a transverse notch of the transverse plate, the moving blocks are sleeved on the transverse screw rod and the sliding rod, the moving blocks are connected with the turnover structure, and the turnover structure moves horizontally through rotation of the transverse screw rod.
The turnover structure comprises a base plate, a turnover cylinder, a guide protrusion, a sliding body and a driving gear, wherein the turnover cylinder is fixedly arranged on the base plate, a telescopic rod of the turnover cylinder is connected with the sliding body, the sliding body is slidably sleeved on the guide protrusion, a linear tooth part is arranged at the bottom of the guide protrusion, the linear tooth part is meshed with the driving gear, and the driving gear is connected with the clamping structure.
In one preferred scheme, the clamping structure is provided with a first clamping rod and a second clamping rod which are arranged in a clamping opposite mode, the first clamping rod and the second clamping rod are provided with supporting roller bodies, the four groups of supporting roller bodies form clamping fastening effects on the sampling test tube, one supporting roller body is connected with a rotating motor, and the rotating motor is used for driving the supporting roller bodies to rotate and correspondingly drive the sampling test tube to rotate.
The beneficial effect of above-mentioned scheme is: for some samples with special materials, such as samples with higher hardness or high adhesiveness and difficult separation, the sampling test tube is enabled to continuously rotate through rotation, so that the cutting process of the samples can be completed in the descending process, and the collection process of the samples can be completed better.
The invention provides a sampling method of a microorganism sampling instrument, which comprises the following steps:
s1: the clamping structure is positioned above an object to be sampled through the actions of the transverse moving unit and the vertical moving unit, a sampling test tube is placed in the clamping structure, the clamping structure clamps and fixes the sampling test tube, and a sampling port of the sampling test tube is arranged downwards;
s2: the clamping structure moves downwards under the driving action of the vertical moving unit, the sampling port of the sampling test tube gradually contacts with the object to be sampled below, and the sampling process of the object to be sampled is completed through the sampling test tube, namely, a part of the object to be sampled enters the sampling test tube;
s3: the turnover structure drives the sampled sampling test tube to turn 180 degrees, so that a sampling port of the sampling test tube is placed upwards;
s4: the horizontal moving unit drives the clamping structure and the sampling test tube to move horizontally and finally move to the position above a test tube rack for storing the sampling test tube, then the clamping structure and the sampling test tube are lowered to the position of the test tube rack below through the vertical moving unit, the clamping structure is converted into a loose state from a clamping state, and the sampling test tube is dropped into a fixing hole of the test tube rack;
the above operation flow is repeated to realize the sampling operation of a plurality of sampling test tubes in batch.
In a preferred embodiment, the method comprises the following steps:
when the driving screw rod rotates, the driving block is correspondingly driven to move along the sliding rod, and the driving block correspondingly drives the transverse moving unit to move in the vertical direction.
In a preferred embodiment, the method comprises the following steps:
when the transverse screw rod rotates, the moving block is correspondingly driven to horizontally move along the sliding rod, and the moving block drives the turnover structure and the clamping structure to horizontally move.
In a preferred embodiment, the method comprises the following steps:
the telescopic link of upset cylinder drives the slider is followed the direction arch moves, and then makes the slider passes through sharp tooth drives drive gear rotates, drive gear drives in the rotation process the clamping structure carries out tilting motion, and then adjusts the orientation of clamping structure and sample test tube through this kind of tilting motion.
In a preferred embodiment, the method comprises the following steps:
when the object to be sampled is sampled through the sampling test tube, the rotating motor drives the supporting roller body to rotate, and the supporting roller body rotates and samples the clamped sampling test tube to finish the sampling work of the object to be sampled.
Drawings
FIG. 1 is a schematic structural view of a microorganism sampling apparatus of the present invention.
FIG. 2 is a schematic structural view of a microorganism sampling apparatus of the present invention.
FIG. 3 is a schematic view of a partial region of a microorganism sampling apparatus according to the present invention.
FIG. 4 is a schematic diagram of the structure of the flip structure region of the microorganism sampling instrument of the present invention.
FIG. 5 is a schematic structural view of a holding structure of the microorganism sampling apparatus of the present invention.
FIG. 6 is a schematic view of another sampling tube of the microbiological sampling apparatus of the present invention.
Detailed Description
First embodiment:
as shown in fig. 1 and 2, the present invention provides a microorganism sampling apparatus, which includes an operation table 10, a frame 11, a vertical moving unit 20, a lateral moving unit 30, and a turnover structure 40.
The operation table 10 is provided with a test tube placing area and a sampling area, the test tube placing area is provided with a fixing frame 12 for storing test tubes, and the sampling area is provided with an object 13 to be sampled.
The frame 11 is detachably fixed to the console 10.
The vertical moving unit 20 is provided on the frame 11.
The lateral moving unit 30 is connected to the vertical moving unit 20, and the vertical moving unit 20 drives the lateral moving unit 30 to move in the vertical direction.
The turning structure 40 is connected to the lateral moving unit 30, and the lateral moving unit 30 can drive the turning structure 40 to move in the lateral direction.
The clamping structure 50 is disposed on the overturning structure 40, and the clamping structure 50 is used for clamping the sampling test tube 60.
The invention provides a sampling method of a microorganism sampling instrument, which comprises the following steps:
s1: the clamping structure 50 is positioned above the object 13 to be sampled by the action of the transverse moving unit 30 and the vertical moving unit 20, an experimenter places one sampling test tube 62 into the clamping structure 50, the clamping structure 50 clamps and fixes the sampling test tube 60, and a sampling port of the sampling test tube 60 is arranged downwards;
s2: the clamping structure 50 moves downwards under the driving action of the vertical moving unit 20, the sampling port of the sampling test tube 60 gradually contacts with the object 13 to be sampled below, and the sampling process of the object 13 to be sampled is completed through the sampling test tube, namely, a part of the object 13 to be sampled enters the sampling test tube 60;
s3: the turnover structure 40 drives the sampled sampling test tube to turn 180 degrees, so that the sampling port of the sampling test tube 60 is upwards placed;
s4: the horizontal moving unit 30 drives the clamping structure 50 and the sampling test tube 60 to move horizontally and finally move to the position above the test tube rack 12 for storing the sampling test tubes, then the clamping structure 50 and the sampling test tube are lowered to the position of the test tube rack 12 below through the vertical moving unit 20, the clamping structure 50 is converted from a clamping state to a loosening state, and the sampling test tube 60 is dropped and placed in a fixing hole of the test tube rack 12;
the above operation flow is repeated to realize the sampling operation of a plurality of sampling test tubes in batch.
Second embodiment:
as shown in fig. 3, on the basis of the above embodiment, the vertical moving unit 20 of the present embodiment has a support plate 21, a driving screw 22, a sliding rod 23, and a driving block 24, where the support plate 21 has a lifting notch 25, the driving screw 22 and the sliding rod 23 are arranged in parallel and are all disposed in the lifting notch 25, the driving block 24 is sleeved on the driving screw 22 and the sliding rod, the driving block 24 is connected with the lateral moving unit 30, and the lateral moving unit 30 is made to move in the vertical direction by the rotation of the driving screw 22. Which comprises the following steps: when the driving screw 22 rotates, the driving block 24 is correspondingly driven to move along the sliding rod 23, and the driving block 24 correspondingly drives the transverse moving unit 30 to move in the vertical direction.
The transverse moving unit 30 is provided with a transverse plate 31, a transverse screw rod 32, a sliding rod 33 and a moving block 34, the transverse screw rod 32 and the sliding rod 33 are arranged in parallel and are arranged in a transverse notch 36 of the transverse plate 31, the moving block 34 is sleeved on the transverse screw rod 32 and the sliding rod 33, the moving block 34 is connected with the turnover structure 40, and the turnover structure 40 moves in the horizontal direction through rotation of the transverse screw rod 32. Which comprises the following steps:
when the transverse screw rod 32 rotates, the moving block 34 is correspondingly driven to horizontally move along the sliding rod 33, and the moving block 34 drives the overturning structure 40 and the clamping structure 50 to horizontally move.
Third embodiment:
as shown in fig. 4, on the basis of the above embodiment, the turnover structure 40 of the present embodiment includes a base plate 41, a turnover cylinder 42, a guide protrusion 43, a sliding body 44, and a driving gear 45, where the turnover cylinder 42 is fixedly disposed on the base plate 41, a telescopic rod of the turnover cylinder 42 is connected with the sliding body 44, the sliding body 44 is slidably sleeved on the guide protrusion 43, a linear tooth portion 46 is disposed at the bottom of the guide protrusion 43, the linear tooth portion 46 is engaged with the driving gear 45, and the driving gear 45 is connected with the clamping structure 50. Which comprises the following steps: the telescopic rod of the turnover air cylinder 42 drives the sliding body 44 to move along the guide protrusion 43, so that the sliding body 44 drives the driving gear 45 to rotate through the linear tooth portion 46, the driving gear 45 drives the clamping structure 50 to perform turnover motion in the rotating process, and the orientation of the clamping structure 50 and the sampling test tube is adjusted through the turnover motion.
Fourth embodiment:
as shown in fig. 5, on the basis of the above embodiment, the clamping structure 50 of the present embodiment has a first clamping lever 51 and a second clamping lever 52 which are arranged in a clampable and opposite manner, the first clamping lever 51 and the second clamping lever 52 have support roller bodies 53 thereon, four sets of support roller bodies 53 form a clamping fastening action on the sampling test tube 60, and one of the support roller bodies 53 is connected with a rotation motor 54, and the rotation motor 54 is used for driving the support roller bodies 53 to rotate and correspondingly drives the sampling test tube 60 to rotate. Which comprises the following steps: when the object 13 to be sampled is sampled through the sampling test tube 60, the rotary motor 54 drives the supporting roller body 53 to rotate, and the supporting roller body 53 rotates and samples the clamped sampling test tube 60 to finish the sampling work of the object 13 to be sampled.
As shown in fig. 6, the sampling test tube 60 includes a plastic protective sleeve 61, the plastic protective sleeve 61 is sleeved on the outer wall of the sampling test tube 60, the plastic protective sleeve 61 can avoid the outer wall of the sampling test tube 60 from contacting the sampled sample in the process of sampling through the test tube, and then the plastic protective sleeve 61 is removed after the sampling is successful. The clamping structure 50 is set to be a first clamping member and a second clamping member, the first clamping member clamps the middle area (the area sleeved by the non-plastic protective sleeve 61) of the sampling test tube 60, the second clamping member clamps the plastic protective sleeve 61, and after the sampling is completed, the second clamping member moves relative to the first clamping member, so that the plastic protective sleeve 61 is separated from the sampling test tube main body 62, and the adhesion of the sampling sample to the outer wall of the test tube can be completely avoided.
In other embodiments, the lateral movement unit can drive the clamping structure and the sampling test tube to the sampling test tube storage area, then complete the grabbing step of the empty test tube in the sampling test tube storage area through the clamping structure by matching of the vertical movement unit, then move the sampling test tube to be sampled on the sample to be sampled for sampling operation, and then put the sampled test tube on the designated test tube rack for fixing so as to prepare for the subsequent microorganism detection flow. Install the movable rail on the operation panel, the direction of movable rail is parallel with the width direction of operation panel, frame 11 slidable sets up on the movable rail, just so realized the position movement to frame 11 in width direction, just so make the clamping structure can carry out position adjustment in width direction, just so can cover bigger sample area, place the sample of waiting of different grade type or kind in a plurality of positions on the operation panel promptly, frame 11 moves on the movable rail, and cooperate the cooperation relation of the vertical mobile unit of transverse moving unit, and can accomplish the process of selectively taking a sample in a plurality of different positions in this region through the clamping structure, it is more convenient to use. The utility model provides a sample collection box, including the support roller body, the inside hollow structure that is of support roller body, the absorption hole is passed through the hollow structure of support roller body and is carried to the recovery box in to the guide tube of low reaches, the process sample to dry particulate matter sample can remain material granule on the test tube outer wall, when placing the test tube of taking a sample on the test-tube rack, the clamping structure can remove along the extending direction of test tube, the clamping area of middle part removes to the position of the sample connection of test tube promptly, can make the support roller body 53 rotate and then make the test tube rotate by means of rotating motor 54 in this process, and inhale the hollow structure of support roller body through the absorption hole with the particulate matter that remains on the test tube wall, then along hollow structure entering guide tube in and carry to the recovery box in.

Claims (9)

1. A microbiological sampling apparatus, comprising:
the test tube placing device comprises an operation table, a test tube placing device and a test tube collecting device, wherein the operation table is provided with a test tube placing area and a sampling area, the test tube placing area is used for placing a fixing frame for storing test tubes, and the sampling area is used for placing an object to be sampled;
the stand is detachably fixed on the operating platform;
the vertical moving unit is arranged on the rack;
the transverse moving unit is connected to the vertical moving unit, and the vertical moving unit drives the transverse moving unit to move in the vertical direction;
the overturning structure is connected to the transverse moving unit, and the transverse moving unit can drive the overturning structure to move in the transverse direction;
the clamping structure is arranged on the overturning structure and is used for clamping the sampling test tube;
the clamping structure is provided with a first clamping rod and a second clamping rod which are arranged in a clamping and opposite manner, the first clamping rod and the second clamping rod are provided with supporting roller bodies, the four groups of supporting roller bodies form clamping and fastening functions on the sampling test tube, one supporting roller body is connected with a rotating motor, and the rotating motor is used for driving the supporting roller bodies to rotate and correspondingly driving the sampling test tube to rotate;
the sampling test tube comprises a plastic protective sleeve, the plastic protective sleeve is sleeved on the outer wall of the sampling test tube, the clamping structure is provided with a first clamping piece and a second clamping piece, the first clamping piece clamps the middle area of the sampling test tube, the second clamping piece clamps the plastic protective sleeve, after sampling is completed, the second clamping piece moves relative to the first clamping piece, and then the plastic protective sleeve is separated from the sampling test tube main body, so that the adhesion effect of a sampling object on the outer wall of the test tube is avoided;
the utility model provides a sample test tube is including the support roller body, the inside hollow structure that is of support roller body, the absorption hole is through the hollow structure of support roller body to the downstream guide tube transport to retrieve in the box, the process sample to dry particulate matter sample can remain material granule on the test tube outer wall, when placing the test tube of taking a sample on the test-tube rack, the clamping structure can remove along the extending direction of test tube, the clamping area of middle part removes the position of the sample connection of test tube promptly, can make the support roller body rotate and then make the test tube rotate by means of rotating motor in this in-process, and inhale the hollow structure of support roller body through the absorption hole with the particulate matter that remains on the test tube wall, then enter into the guide tube along hollow structure and in the transport to retrieve the box.
2. A microbiological sampling apparatus according to claim 1, wherein,
the vertical moving unit is provided with a supporting plate, a driving screw rod, a sliding rod and a driving block, the supporting plate is provided with a lifting notch, the driving screw rod and the sliding rod are arranged in parallel and are all arranged in the lifting notch, the driving screw rod and the sliding rod are sleeved with the driving block, the driving block is connected with the transverse moving unit, and the transverse moving unit moves in the vertical direction through the rotation of the driving screw rod.
3. A microbiological sampling apparatus according to claim 2, wherein,
the transverse moving unit is provided with a transverse plate, a transverse screw rod, a sliding rod and moving blocks, wherein the transverse screw rod is arranged in parallel with the sliding rod and is arranged in a transverse notch of the transverse plate, the moving blocks are sleeved on the transverse screw rod and the sliding rod, the moving blocks are connected with the turnover structure, and the turnover structure moves horizontally through rotation of the transverse screw rod.
4. A microbiological sampling apparatus according to claim 3, wherein,
the turnover structure comprises a base plate, a turnover cylinder, a guide protrusion, a sliding body and a driving gear, wherein the turnover cylinder is fixedly arranged on the base plate, a telescopic rod of the turnover cylinder is connected with the sliding body, the sliding body is slidably sleeved on the guide protrusion, a linear tooth part is arranged at the bottom of the guide protrusion, the linear tooth part is meshed with the driving gear, and the driving gear is connected with the clamping structure.
5. The method for sampling a microorganism sampling instrument according to claim 4, comprising the steps of:
s1: the clamping structure is positioned above an object to be sampled through the actions of the transverse moving unit and the vertical moving unit, a sampling test tube is placed in the clamping structure, the clamping structure clamps and fixes the sampling test tube, and a sampling port of the sampling test tube is arranged downwards;
s2: the clamping structure moves downwards under the driving action of the vertical moving unit, the sampling port of the sampling test tube gradually contacts with the object to be sampled below, and the sampling process of the object to be sampled is completed through the sampling test tube, namely, a part of the object to be sampled enters the sampling test tube;
s3: the turnover structure drives the sampled sampling test tube to turn 180 degrees, so that a sampling port of the sampling test tube is placed upwards;
s4: the horizontal moving unit drives the clamping structure and the sampling test tube to move horizontally and finally move to the position above a test tube rack for storing the sampling test tube, then the clamping structure and the sampling test tube are lowered to the position of the test tube rack below through the vertical moving unit, the clamping structure is converted into a loose state from a clamping state, and the sampling test tube is dropped into a fixing hole of the test tube rack;
the above operation flow is repeated to realize the sampling operation of a plurality of sampling test tubes in batch.
6. The method for sampling a microbiological sampling apparatus according to claim 5, comprising the steps of:
when the driving screw rod rotates, the driving block is correspondingly driven to move along the sliding rod, and the driving block correspondingly drives the transverse moving unit to move in the vertical direction.
7. The method for sampling a microbiological sampling apparatus according to claim 6, comprising the steps of:
when the transverse screw rod rotates, the moving block is correspondingly driven to horizontally move along the sliding rod, and the moving block drives the turnover structure and the clamping structure to horizontally move.
8. The method for sampling a microbiological sampling apparatus according to claim 7, comprising the steps of:
the telescopic link of upset cylinder drives the slider is followed the direction arch moves, and then makes the slider passes through sharp tooth drives drive gear rotates, drive gear drives in the rotation process the clamping structure carries out tilting motion, and then adjusts the orientation of clamping structure and sample test tube through this kind of tilting motion.
9. The method of sampling a microbiological sampling apparatus according to claim 8, comprising the steps of:
when the object to be sampled is sampled through the sampling test tube, the rotating motor drives the supporting roller body to rotate, and the supporting roller body rotates and samples the clamped sampling test tube to finish the sampling work of the object to be sampled.
CN202110393711.XA 2021-04-13 2021-04-13 Microorganism sampling instrument and method Active CN113174320B (en)

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