CN209508277U - Laser microprobe dating instrument - Google Patents

Laser microprobe dating instrument Download PDF

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Publication number
CN209508277U
CN209508277U CN201821794864.5U CN201821794864U CN209508277U CN 209508277 U CN209508277 U CN 209508277U CN 201821794864 U CN201821794864 U CN 201821794864U CN 209508277 U CN209508277 U CN 209508277U
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China
Prior art keywords
light
mirror
laser
wavelength
rupture
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CN201821794864.5U
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Chinese (zh)
Inventor
胡舜迪
闻路红
甘剑勤
郭荣
洪欢欢
陈腊
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GUANGZHOU HUAYUEHANG MEDICAL TECHNOLOGY Co.,Ltd.
Original Assignee
Ningbo Huayi Ningchuang Intelligent Science & Technology Co Ltd
Guangzhou Huayuehang Medical Technology Co Ltd
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Abstract

The utility model provides a kind of laser microprobe dating instrument, including laser, object lens;Object lens include: the optical channel having in shell along casing center axis;Laser is arranged in shell, and the wavelength of the rupture of membranes light of outgoing is λ1,Laser is 0 perpendicular to the projected area on the section on central axis optical channel;Wavelength convert component is arranged in shell, and in the emergent light optical path in laser;Wavelength convert component converts the wavelength of rupture of membranes light, the wavelength X of conversion light as needed2∈[380nm,780nm];Filter switch, and the switch that filters is λ for preventing wavelength as needed1Rupture of membranes light emission to embryo;Rupture of membranes light and/or conversion light are reflected into reflective mirror by two-dimensional scanning mirror unit;The projected area of two-dimensional scanning mirror unit and wavelength convert component on section is 0;Reflective mirror is arranged in optical channel;Reflected light on reflective mirror is transmitted in optical channel.The utility model, which has, controls accurate, advantages of simple structure and simple.

Description

Laser microprobe dating instrument
Technical field
The utility model relates to Embryo Cultures, in particular to laser microprobe dating instrument.
Background technique
Laser microprobe dating instrument is a kind of equipment for being scanned cutting operation to embryo by emission pulse laser beam.Swash In light rupture of membranes technology, the design to laser and instruction light is a very crucial job.Since laser is usually infrared light, people Eye it is invisible, indicate that the design of light can help operator that the position of laser beam is accurately positioned, if instruction light with The positional relationship processing of laser is bad, then beam of laser energy can be made to get to other positions, cause to damage to embryo.It is existing at present Scheme is main are as follows:
The first scheme: laser and instruction light are individually placed to object lens two sides, a face reflecting mirror and object are put among object lens The central axis of mirror fixation at a certain angle, the laser that laser issues, is incident on after being incident on the head-on reflection of reflecting mirror Object lens, guidance light are incident on eyepiece after being incident on the reverse side reflection of reflecting mirror.Guide light and laser all in the central axis of object lens On, it is contrary.The deficiency of this method is:
1. needing to use two light source variables, the difficulty and error of adjustment will increase in the process of adjustment, it is not easily-controllable System.
2. can only carry out laser single-point shooting, if to realize cutting must by way of manual moving stage, It cannot achieve automatically scanning cutting.
Second scheme: scanning system is mounted on the fluorescence channel of inverted microscope, and deficiency is: occupying fluorescence function It can use, it is very inconvenient.
Utility model content
To solve the deficiency in above-mentioned prior art, the utility model provides a kind of laser microprobe dating instrument, real Show two-dimensional scanning, and is not take up fluorescence channel.
The purpose of this utility model is achieved through the following technical solutions:
A kind of laser microprobe dating instrument, the laser microprobe dating instrument include laser, object lens;The object lens Include:
Shell, the interior optical channel having along the casing center axis of the shell;The laser is arranged in shell In vivo, the wavelength of the rupture of membranes light of outgoing is λ1,The laser the optical channel perpendicular to The projected area on section on the central axis is 0;
Wavelength convert component, the wavelength convert component are arranged in the shell, and are in the outgoing of the laser In light optical path;The wavelength convert component converts the wavelength of rupture of membranes light, the wavelength X of conversion light as needed2∈[380nm, 780nm];
Filter switch, and the optical filtering switch is λ for preventing wavelength as needed1Rupture of membranes light emission to embryo;
Rupture of membranes light and/or conversion light are reflected into reflective mirror by two-dimensional scanning mirror unit, the two-dimensional scanning mirror unit;It is described The projected area of two-dimensional scanning mirror unit and wavelength convert component on the section is 0;
Reflective mirror, the reflective mirror are arranged in the optical channel;Reflected light on reflective mirror is in the optical channel Interior transmission.
According to above-mentioned laser microprobe dating instrument, it is preferable that the reflective mirror is greater than the reflectivity of the rupture of membranes light 90%, which is greater than the reflectivity to the conversion light.
According to above-mentioned laser microprobe dating instrument, it is preferable that the reflective mirror is to wavelength X3The reflectivity of light be less than 1%, λ3∈[380nm,λ2)∪(λ2, 780nm].
According to above-mentioned laser microprobe dating instrument, optionally, the object lens further comprise:
Mirror unit, the mirror unit are arranged in the shell, and the conversion light is reflected into two-dimensional scanning Mirror unit;Projected area of the mirror unit on the section is 0.
According to above-mentioned laser microprobe dating instrument, optionally, the mirror unit includes:
First reflecting mirror, the emergent light are successively reflected by the first reflecting mirror and the second reflecting mirror;
Second reflecting mirror, first reflecting mirror and the second reflecting mirror are arranged along the circumferential direction of the optical channel.
According to above-mentioned laser microprobe dating instrument, optionally, the two-dimensional scanning mirror unit includes:
First scanning mirror, first scanning mirror and the second scanning mirror are setting up and down;
The rotary shaft of second scanning mirror, the first scanning mirror and the second scanning mirror is mutually perpendicular to.
According to above-mentioned laser microprobe dating instrument, it is preferable that the wavelength convert component is frequency multiplication component.
According to above-mentioned laser microprobe dating instrument, it is preferable that wavelength X1, λ1∈[1400nm,1500nm]。
Compared with prior art, the utility model has the beneficial effect that
1. object lens have both laser two-dimension scanning function, accurate positioning;
2. each component and optical path are not take up fluorescence channel in use process.
Detailed description of the invention
Referring to attached drawing, the disclosure of the utility model will be easier to understand.Skilled addressee readily understands that Be: these attached drawings are used only for illustrating the technical solution of the utility model, and are not intended to the protection to the utility model Range is construed as limiting.In figure:
Fig. 1 is the structure diagram according to the laser microprobe dating instrument of the utility model embodiment 1;
Fig. 2 is the structure diagram according to the laser microprobe dating instrument of the utility model embodiment 2.
Specific embodiment
Fig. 1-2 and following description describe the optional embodiments of the utility model how to instruct those skilled in the art Implement and reproduce the utility model.In order to instruct technical solutions of the utility model, it has simplified or omitted some conventional aspects.This Field technical staff should be understood that the variation or replacement from these embodiments will be in the scope of the utility model.This field Technical staff should be understood that following characteristics can be combined in various ways to form multiple modifications of the utility model.As a result, originally Utility model is not limited to following optional embodiments, and is only limited by the claims and their equivalents.
Embodiment 1:
Fig. 1 schematically illustrates the structure diagram of the laser microprobe dating instrument of the utility model embodiment 1, such as Fig. 1 institute Show, the laser microprobe dating instrument includes:
Laser, the wavelength for the rupture of membranes light that the laser issues are λ1, λ1∈ [1400nm, 1500nm],
Object lens, the object lens include:
Shell, the interior optical channel having along the casing center axis of the shell;The laser is arranged in shell In vivo, the laser is 0 perpendicular to projected area on the section on the central axis in the optical channel;
Wavelength convert component, such as frequency multiplication component, the wavelength convert component is arranged in the shell, and swashs in described In the emergent light optical path of light device;The wavelength convert component converts the wavelength of rupture of membranes light, the wavelength X of conversion light as needed2 ∈[380nm,780nm];The transfer efficiency of wavelength convert component is adjustable;
Filter switch, and it is λ that the optical filtering switch filters out wavelength as needed1Rupture of membranes light;
Two-dimensional scanning mirror unit, such as MEMS scanning galvanometer, the two-dimensional scanning mirror unit is by rupture of membranes light and/or conversion light It is reflected into reflective mirror;The projected area of the two-dimensional scanning mirror unit and wavelength convert component on the section is 0, and laser Optical path and optical channel between device, wavelength convert component and two-dimensional scanning mirror unit are not intersected;
Reflective mirror, the reflective mirror are arranged in the optical channel;Reflected light on reflective mirror is in the optical channel Interior transmission;The reflective mirror is greater than 90%, such as 99% to the reflectivity of the rupture of membranes light, which is greater than to the conversion light Reflectivity, such as 40-60%;And the reflective mirror is to wavelength X3Light reflectivity less than 1%, λ3∈[380nm,λ2)∪ (λ2, 780nm].
The working method of the laser microprobe dating instrument of the utility model embodiment, the working method the following steps are included:
(A1) the rupture of membranes light that laser issues is incident on wavelength convert component;
(A2) wavelength convert component works, by the wavelength X of the rupture of membranes light1It is transformed into wavelength X2∈[380nm,780nm];
(A3) conversion light is mapped on embryo after two-dimensional scanning mirror unit, reflective mirror;
Two-dimensional scanning mirror unit is adjusted, so that conversion light is mapped to the designated position of embryo;
The switch that filters is opened, and wavelength X is filtered out1Rupture of membranes light, prevent the indicating positions stage damage embryo;
(A4) working condition for adjusting wavelength convert component, when the rupture of membranes light passes through the wavelength convert component, few portion Point rupture of membranes light is converted to conversion light, and most of constant the passes through wavelength convert component;Rupture of membranes light and conversion light successively pass through two dimension The reflection for scanning mirror unit, reflective mirror, is mapped to the designated position of embryo.
Embodiment 2:
Fig. 2 schematically illustrates the structure diagram of the laser microprobe dating instrument of the utility model embodiment 2, such as Fig. 2 institute Show, the laser microprobe dating instrument includes:
Laser, the wavelength for the rupture of membranes light that the laser issues are λ1, λ1∈ [1400nm, 1500nm],Laser power range is 200mW-600mW;
Object lens, the object lens include:
Shell, the interior optical channel having along the casing center axis of the shell;The laser is arranged in shell In vivo, the laser is 0 perpendicular to projected area on the section on the central axis in the optical channel;
Wavelength convert component, such as frequency multiplication component, the wavelength convert component is arranged in the shell, and swashs in described In the emergent light optical path of light device;The wavelength convert component converts the wavelength of rupture of membranes light, the wavelength X of conversion light as needed2 ∈[380nm,780nm];The transfer efficiency of wavelength convert component is adjustable;
Filter switch, and it is λ that the optical filtering switch filters out wavelength as needed1Rupture of membranes light;
Mirror unit, the mirror unit are arranged in the shell, and the conversion light is reflected into two-dimensional scanning Mirror unit;Projected area of the mirror unit on the section is 0;The mirror unit includes:
First reflecting mirror, the emergent light are successively reflected by the first reflecting mirror and the second reflecting mirror;
Second reflecting mirror, first reflecting mirror and the second reflecting mirror are arranged along the circumferential direction of the optical channel;
Rupture of membranes light and/or conversion light are reflected into reflective mirror by two-dimensional scanning mirror unit, the two-dimensional scanning mirror unit;It is described The projected area of two-dimensional scanning mirror unit and wavelength convert component on the section be 0, and laser, wavelength convert component, Optical path and optical channel between mirror unit and two-dimensional scanning mirror unit are not intersected;The two-dimensional scanning mirror unit packet It includes:
First scanning mirror, first scanning mirror and the second scanning mirror are setting up and down;First scanning mirror, the first reflection Mirror and the second reflecting mirror are arranged along the circumferential direction of the optical channel;
The rotary shaft of second scanning mirror, the first scanning mirror and the second scanning mirror is mutually perpendicular to, to realize two-dimensional scanning function Energy;
Reflective mirror, the reflective mirror are arranged in the optical channel;Reflected light on reflective mirror is in the optical channel Interior transmission;The reflective mirror is greater than 90%, such as 99% to the reflectivity of the rupture of membranes light, which is greater than to the conversion light Reflectivity, such as 40-60%;And the reflective mirror is to wavelength X3Light reflectivity less than 1%, λ3∈[380nm,λ2)∪ (λ2, 780nm].
The working method of the laser microprobe dating instrument of the utility model embodiment, the working method the following steps are included:
(A1) the rupture of membranes light that laser issues is incident on wavelength convert component;
(A2) wavelength convert component works, by the wavelength X of the rupture of membranes light1It is transformed into wavelength X2∈[380nm,780nm];
(A3) conversion light successively by the first reflecting mirror, the second reflecting mirror reflection after be incident on the first scanning mirror;
It is mapped on embryo after two-dimensional scanning mirror unit, reflective mirror;
Two-dimensional scanning mirror unit is adjusted, so that conversion light is mapped to the designated position of embryo;
The switch that filters is opened, and wavelength X is filtered out1Rupture of membranes light, prevent the indicating positions stage damage embryo;
(A4) working condition for adjusting wavelength convert component, when the rupture of membranes light passes through the wavelength convert component, few portion Point rupture of membranes light is converted to conversion light, and most of constant the passes through wavelength convert component;Rupture of membranes light and conversion light successively pass through two dimension The reflection for scanning mirror unit, reflective mirror, is mapped to the designated position of embryo.
Embodiment 3:
According to the laser microprobe dating instrument of the utility model embodiment 2 and its application examples of working method.
In the application examples, the wavelength X of the rupture of membranes light of semiconductor laser outgoing1For 1480nm ± 20nm, power 500 ±50mW;Wavelength convert component uses frequency multiplication component, the wavelength X of conversion light2For 740nm ± 10nm;First scanning mirror, One reflecting mirror and the second reflecting mirror are arranged along the circumferential direction of the optical channel, between the main shaft of optical path be in perpendicular to shell Central axis plane on;First scanning mirror is rotated along first rotating shaft, and the second scanning mirror is rotated along the second shaft, and first Shaft and the second shaft are mutually perpendicular to;Shell cylindrical part, internal void are divided into optical channel, diameter R1;In shell Between partially have larger section of internal diameter, diameter R2, R2> R1;Above-mentioned laser, wavelength convert component, mirror unit and two dimension Scanning mirror unit and between optical path be in larger section of internal diameter, and be in R2> R > R1Region in, namely in the light The projected area learned in the section perpendicular to the central axis of shell in channel is 0.

Claims (8)

1. a kind of laser microprobe dating instrument, the laser microprobe dating instrument includes laser, object lens;It is characterized by: institute Stating object lens includes:
Shell, the interior optical channel having along the casing center axis of the shell;The laser is arranged in shell, The wavelength of the rupture of membranes light of outgoing is λ1,The laser is in the optical channel perpendicular to described The projected area on section on central axis is 0;
Wavelength convert component, the wavelength convert component are arranged in the shell, and are in the emergent light light of the laser On the road;The wavelength convert component converts the wavelength of rupture of membranes light, the wavelength X of conversion light as needed2∈[380nm, 780nm];
Filter switch, and the optical filtering switch is λ for preventing wavelength as needed1Rupture of membranes light emission to embryo;
Rupture of membranes light and/or conversion light are reflected into reflective mirror by two-dimensional scanning mirror unit, the two-dimensional scanning mirror unit;The two dimension Scanning the projected area of mirror unit and wavelength convert component on the section is 0;
Reflective mirror, the reflective mirror are arranged in the optical channel;Reflected light on reflective mirror passes in the optical channel It is defeated.
2. laser microprobe dating instrument according to claim 1, it is characterised in that: the reflective mirror is anti-to the rupture of membranes light Rate is penetrated greater than 90%, which is greater than the reflectivity to the conversion light.
3. laser microprobe dating instrument according to claim 2, it is characterised in that: the reflective mirror is to wavelength X3Light it is anti- Rate is penetrated less than 1%, λ3∈[380nm,λ2∪(λ2, 780nm].
4. laser microprobe dating instrument according to claim 1, it is characterised in that: the object lens further comprise:
Mirror unit, the mirror unit are arranged in the shell, and the conversion light is reflected into Scan mirror list Member;Projected area of the mirror unit on the section is 0.
5. laser microprobe dating instrument according to claim 4, it is characterised in that: the mirror unit includes:
First reflecting mirror, the emergent light are successively reflected by the first reflecting mirror and the second reflecting mirror;
Second reflecting mirror, first reflecting mirror and the second reflecting mirror are arranged along the circumferential direction of the optical channel.
6. laser microprobe dating instrument according to claim 5, it is characterised in that: the two-dimensional scanning mirror unit includes:
First scanning mirror, first scanning mirror and the second scanning mirror are setting up and down;First reflecting mirror, the second reflecting mirror and First scanning mirror is arranged along the circumferential direction of the optical channel;
The rotary shaft of second scanning mirror, the first scanning mirror and the second scanning mirror is mutually perpendicular to.
7. laser microprobe dating instrument according to claim 1, it is characterised in that: the wavelength convert component is frequency multiplication portion Part.
8. laser microprobe dating instrument according to claim 1, it is characterised in that: wavelength X1, λ1∈[1400nm,1500nm]。
CN201821794864.5U 2018-03-26 2018-11-01 Laser microprobe dating instrument Active CN209508277U (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201820414817 2018-03-26
CN2018204148177 2018-03-26

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Publication Number Publication Date
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109251857A (en) * 2018-03-26 2019-01-22 宁波华仪宁创智能科技有限公司 Laser microprobe dating instrument and its working method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109251857A (en) * 2018-03-26 2019-01-22 宁波华仪宁创智能科技有限公司 Laser microprobe dating instrument and its working method
CN109251857B (en) * 2018-03-26 2023-09-05 广州市华粤行医疗科技有限公司 Laser micro-cutting instrument and working method thereof

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GR01 Patent grant
GR01 Patent grant
TR01 Transfer of patent right

Effective date of registration: 20220215

Address after: 510700 First Floor, C2 Building, 11 Kaiyuan Avenue, Huangpu District, Guangzhou City, Guangdong Province

Patentee after: GUANGZHOU HUAYUEHANG MEDICAL TECHNOLOGY Co.,Ltd.

Address before: 510700 First Floor, C2 Building, 11 Kaiyuan Avenue, Huangpu District, Guangzhou City, Guangdong Province

Patentee before: GUANGZHOU HUAYUEHANG MEDICAL TECHNOLOGY Co.,Ltd.

Patentee before: Ningbo Huayi ningchuang Intelligent Technology Co., Ltd

TR01 Transfer of patent right