CN108614351B - Microscope with rotatable objective lens - Google Patents
Microscope with rotatable objective lens Download PDFInfo
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- CN108614351B CN108614351B CN201611141424.5A CN201611141424A CN108614351B CN 108614351 B CN108614351 B CN 108614351B CN 201611141424 A CN201611141424 A CN 201611141424A CN 108614351 B CN108614351 B CN 108614351B
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/248—Base structure objective (or ocular) turrets
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/02—Objectives
- G02B21/04—Objectives involving mirrors
Abstract
The invention provides a microscope with a rotatable objective lens, which comprises a microscope, a light source part, a scanning part, an objective lens, a photoelectric detection device and an objective lens light path adjusting part, wherein the microscope is provided with the light source part, the scanning part, the objective lens and the photoelectric detection device, the objective lens light path adjusting part is arranged between the objective lens and the photoelectric detection device, and the objective lens light path adjusting part is formed by sequentially forming a first rotating frame, a first right-angle optical adjusting frame, a second rotating frame, a first relay lens, a second right-angle optical adjusting frame, a third right-angle optical adjusting frame, a second relay lens and a cage-shaped cube. The objective lens is simple in structure, can rotate in multiple degrees of freedom with the focus as the center under the condition of ensuring that the optical path is unchanged, does not need to perform corresponding auxiliary adjustment on other optical paths in a microscope, effectively realizes imaging experiment observation on a living biological sample, is low in cost, and can be matched with a full-field or scanning microscope for use.
Description
Technical Field
The invention relates to the technical field of microscopes, in particular to a microscope with a rotatable objective lens.
Background
At present, a common microscope is mainly used for experimental observation of a slice sample, and a fixed imaging light path of the microscope is kept in a state vertical to a horizontal plane where the sample is located no matter the microscope is placed rightly or upside down in the process. However, for the imaging of the living biological sample, since the external shape is usually a specific three-dimensional shape, the surface is non-planar, and the imaging portion is various, if the objective lens is perpendicular to the imaging surface, the imaging of the living biological sample is often skewed, and when the living biological sample is horizontally placed, it is difficult to achieve the perpendicular of the objective lens and the imaging surface, and the microscope cannot normally work, so the fixed imaging optical path of the ordinary microscope is difficult to meet the imaging requirement of the living biological sample. Therefore, in order to realize experimental observation of living biological samples, various microscopic imaging techniques are rapidly developed, including two major categories, namely full-field imaging and flying-spot scanning imaging. Full-field imaging includes full-field optical coherence tomography and the like; the scanning imaging comprises nonlinear optical imaging such as multi-photon excited fluorescence, second/third harmonic generation, stimulated Raman scattering, coherent anti-Stokes Raman scattering and the like. However, the two existing imaging techniques have the following problems in the practical use process:
1. for full-field optical coherence tomography, in order to ensure the experimental observation of the living biological sample, if observation is performed in a mode of rotating the objective lens, the optical path of the rotating optical path on the sample arm is changed, so that corresponding adjustment is required to be performed on the optical path of the reference arm, accurate imaging can be realized, and the adjustment operation process is troublesome.
2. In a scanning microscope, an objective lens is rotated during experimental observation, if the optical path of a rotating optical path is changed at the moment, the optical path between a sleeve lens and the objective lens is changed, the optical path between the scanning lens and a scanning head needs to be correspondingly adjusted, and the other optical paths in the microscope are very difficult to be correspondingly adjusted while the objective lens is rotated, and only the Bergamo II of Thorlabs company in America can realize similar functions in a commercial microscope product, but only the angular rotation in the range of 0-95 degrees can be ensured.
Disclosure of Invention
The invention mainly aims to solve the problems in the prior art, and provides a microscope with a rotatable objective lens, which has a simple structure, can rotate with multiple degrees of freedom by taking a focus as a center under the condition of ensuring that the optical path is not changed, thereby effectively realizing the imaging experiment observation of a living biological sample without carrying out corresponding auxiliary adjustment on other optical paths in the microscope, has low cost, and can be matched with a full-field or scanning microscope for use.
In order to solve the technical problems, the invention adopts the technical scheme that: a microscope with a rotatable objective lens comprises a microscope, a light source part, a scanning part, an objective lens, a photoelectric detection device and an objective lens light path adjusting part, wherein the light source part, the scanning part, the objective lens and the photoelectric detection device are arranged on the microscope, the scanning part enables incident light beams of the light source part to enter the objective lens light path adjusting part, the objective lens light path adjusting part is arranged between the objective lens and the photoelectric detection device, and light information is output to a host of the microscope through the photoelectric detection device so as to control the light beam condition of the light source part to better meet detection requirements;
the scanning part is arranged to comprise a scanning head, a scanning lens and a sleeve lens, a scanning reflector is arranged on the scanning head, the scanning lens and the sleeve lens are sequentially arranged behind the scanning reflector, and the scanning reflector is used for guiding the light beam of the light source part so that the light beam of the light source part can enter the objective lens light path adjusting part through the collimation of the scanning lens and the sleeve lens;
the objective optical path adjusting part is composed of a first rotating frame, a first right-angle optical adjusting frame, a second rotating frame, a first relay lens, a second right-angle optical adjusting frame, a third right-angle optical adjusting frame, a second relay lens and a cage-shaped cube in sequence, a rotating shaft of the first rotating frame is arranged to be coincident with an imaging optical axis of the microscope, the first rotating frame is arranged on a right-angle edge of the first right-angle optical adjusting frame, the second rotating frame is arranged on the other right-angle edge of the first right-angle optical adjusting frame, a rotating shaft of the second rotating frame is arranged to pass through a focus of the objective lens, reflecting mirrors are arranged on oblique edges of the first right-angle optical adjusting frame, the second right-angle optical adjusting frame and the third right-angle optical adjusting frame, the reflecting mirrors are used for realizing 90-degree light beam rotation, and the reflecting mirrors can be selected according to actual requirements, The device comprises a first relay lens, a second relay lens, a cage-shaped cube, a photoelectric detection device, an objective lens, a light source part and a scanning head, wherein the first relay lens and the second relay lens are both arranged to be of an achromatic structure or a apochromatic structure, the upper end and the lower end of the cage-shaped cube are respectively provided with the photoelectric detection device and the objective lens, the cage-shaped cube feeds back light beam information to a host of the microscope through the photoelectric detection device and is matched with the actual detection condition of a living biological sample in the objective lens to control the light source part and the scanning head to realize the adjustment of the working condition of the light beam, and a.
Further, the front focal point of the first relay lens and the front focal point of the second relay lens are both arranged at the rear focal point of the sleeve lens, the first relay lens and the second relay lens are both arranged to convert the angle scanning collimated light beam of the sleeve lens into a parallel focusing light beam, and the first relay lens and the second relay lens are both arranged as a lens or a lens combination of any material, wavelength, shape and focal length according to requirements.
Further, the transmission wavelengths of the first relay lens and the second relay lens are both set to be 100nm to 2 um.
Furthermore, the first rotating frame and the second rotating frame are both set to rotate for 360 degrees, the first rotating frame and the second rotating frame are both set to be driven manually or electrically, and meanwhile, the rotation of the first rotating frame and the second rotating frame is in a continuous or stepping mode, and the first rotating frame and the second rotating frame are provided with locking devices to be fixed at any positions.
Furthermore, the inner cavity of the cage-shaped cube is provided with a dichroic mirror and an optical filter, and the dichroic mirror is matched with the optical filter and other devices of the objective lens light path adjusting part, so that NDD detection of the microscope can be realized.
Furthermore, the inner cavity of the cage-shaped cube is set as a reflector, and the reflector is matched with other devices of the objective optical path adjusting part, so that the requirement of common experimental detection of the microscope can be realized.
Further, the surface of the reflector is plated with a high-transmittance film, and the transmission wavelength of the reflector is set to be 100nm to 2 um.
Further, the objective lens is arranged on the microscope through an objective lens interface adapter, the objective lens adapter is further arranged on the objective lens, the first rotating frame is connected and arranged on the objective lens interface adapter, the objective lens interface adapter is electrically connected with the objective lens adapter, the objective lens adapter is connected with the cage-shaped cube, the objective lens interface adapter is arranged to enable the objective lenses of different specifications to be well connected with the microscope, and the objective lens light path adjusting part ensures that the objective lenses can accurately image the living biological samples.
The invention has the advantages and positive effects that:
(1) through the design of the light source part, the scanning part, the objective lens, the photoelectric detection device and the objective lens light path adjusting part, the microscope can realize good and accurate imaging of the living biological sample, and the photoelectric detection device outputs light information to a host of the microscope so as to control the light beam condition of the light source part to better meet the detection requirement.
(2) The objective lens optical path adjusting part is arranged behind the objective lens or the objective lens adapter thereof, so that the first rotating frame, the first right-angle optical adjusting frame, the second rotating frame, the first relay lens, the second right-angle optical adjusting frame, the third right-angle optical adjusting frame and the second relay lens are matched with the cage-shaped cube, the objective lens can rotate with a focus as a center with multiple degrees of freedom under the condition of ensuring that the optical path is unchanged, corresponding auxiliary adjustment of other optical paths in a microscope and repeated focusing of the objective lens are not needed, imaging experiment observation of a living biological sample is effectively realized, the cost is low, the objective lens optical path adjusting part can be matched with a full-field or scanning microscope, and the objective lens optical path adjusting part is applicable.
(3) Through the achromatic structure or apochromatic structure of the first relay lens and the second relay lens, the imaging clarity is further ensured, and the imaging quality is improved.
(4) The dichroic mirror and the optical filter or the reflecting mirror are placed in the inner cavity of the cage-shaped cube, so that NDD detection of a microscope or detection requirements of common experiments can be realized.
(5) The objective lenses with different specifications can be well connected with the microscope through the objective lens interface adapter.
Drawings
FIG. 1 is a block diagram illustrating the structure of the present invention.
Fig. 2 is a schematic view of the optical path of fig. 1 in operation.
In the figure: 10-microscope, 20-light source part, 30-scanning part, 301-scanning head, 302-scanning lens, 303-telescopic lens, 304-scanning reflector, 40-objective lens, 401-objective lens interface adapter, 402-objective lens adapter, 50-photoelectric detection device, 60-objective lens optical path adjusting part, 601-first rotating frame, 602-first right-angle optical adjusting frame, 603-second rotating frame, 604-first relay lens, 605-second right-angle optical adjusting frame, 606-third right-angle optical adjusting frame, 607-second relay lens and 608-cage cube.
Detailed Description
For a better understanding of the present invention, reference is made to the following detailed description and accompanying drawings that illustrate the invention.
As shown in fig. 1 and fig. 2, a microscope with a rotatable objective lens includes a microscope 10, a light source 20, a scanning portion 30, an objective lens 40, a photodetection device 50, and an objective lens optical path adjusting portion 60, wherein the microscope 10 is provided with the light source 20, the scanning portion 30, the objective lens 40, and the photodetection device 50, the scanning portion 30 realizes that an incident light beam of the light source 20 enters the objective lens optical path adjusting portion 60, the objective lens optical path adjusting portion 60 is disposed between the objective lens 40 and the photodetection device 50, and light information is output to a host of the microscope 10 through the photodetection device 50, so as to control the light beam condition of the light source 20 to better meet detection requirements.
The scanning section 30 is configured to include a scanning head 301, a scanning lens 302 and a sleeve lens 303, the scanning head 301 is provided with a scanning mirror 304, the scanning lens 302 and the sleeve lens 303 are sequentially provided behind the scanning mirror 304, and the scanning mirror 304 is configured to guide the light beam of the light source section 20 so that the light beam of the light source section 20 can enter the objective optical path adjusting section 60 through collimation of the scanning lens 302 and the sleeve lens 303.
The objective optical path adjusting section 60 is configured to be composed of a first rotating frame 601, a first right-angle optical adjusting frame 602, a second rotating frame 603, a first relay lens 604, a second right-angle optical adjusting frame 605, a third right-angle optical adjusting frame 606, a second relay lens 607, and a cage cube 608 in this order, a rotation axis of the first rotating frame 601 is configured to coincide with an imaging optical axis of the microscope 10, the first rotating frame 601 is configured on a right-angle side of the first right-angle optical adjusting frame 602, and the second rotating frame 603 is configured on the other right-angle side of the first right-angle optical adjusting frame 602, a rotation axis of the second rotating frame 603 is configured to pass through a focal point of the objective lens 40, mirrors are configured on oblique sides of the first right-angle optical adjusting frame 602, the second right-angle optical adjusting frame 605, and the third right-angle optical adjusting frame 606, and are configured to rotate the light beam by 90 degrees, and the mirrors may be selected according to actual needs, Wavelength and shape, the first relay lens 604 and the second relay lens 607 are both set to be achromatic structure or apochromatic structure, the upper and lower ends of the cage cube 608 are respectively provided with the photoelectric detection device 50 and the objective lens 40, the cage cube 608 feeds back light beam information from the photoelectric detection device 50 to the host of the microscope 10, and in cooperation with the actual detection condition of the living biological sample in the objective lens 40, the light source part 20 and the scanning head 301 are controlled to adjust the working condition of the light beam, so as to ensure good experimental observation effect.
The front focal point of the first relay lens 604 and the front focal point of the second relay lens 607 are both set at the rear focal point of the sleeve lens 303, the first relay lens 604 and the second relay lens 607 are both set to convert the angle-scanning collimated light beam of the sleeve lens 303 into a parallel focused light beam, and the first relay lens 604 and the second relay lens 607 are both set as lenses or lens combinations of any material, wavelength, shape, and focal length as required.
The transmission wavelengths of the first relay lens 604 and the second relay lens 607 are set to 100nm to 2 um.
The first rotating frame 601 and the second rotating frame 603 are both set to rotate by 360 degrees, the first rotating frame 601 and the second rotating frame 603 are both set to be driven manually or electrically, and the rotation is in a continuous or stepping mode, and a locking device is provided to fix the first rotating frame 601 and the second rotating frame 603 at any position.
The inner cavity of the cage cube 608 is configured as a dichroic mirror and a filter, which cooperate with the filters and other components of the objective optical path adjustment portion 60 to enable NDD detection of the microscope 10.
The interior cavity of the cage cube 608 is configured as a mirror that cooperates with other components of the objective optic path adjustment portion 40 to enable common experimental testing needs of the microscope 10.
The surface of the reflector is plated with a high-transmittance film, and the transmission wavelength of the reflector is set to be 100nm to 2 um.
The objective lens 40 is disposed on the microscope 10 through the objective lens interface adapter 401, and the objective lens 40 is further disposed with an objective lens adapter 402, the objective lens interface adapter 401 is disposed with the first rotating frame 601, and the objective lens interface adapter 401 is disposed with the objective lens adapter 402, the objective lens adapter 402 is disposed with the cage cube 608, the objective lens interface adapter 401 is disposed to make the objective lenses 40 of different specifications connect well with the microscope 10, and the objective lens optical path adjusting portion 60 ensures the objective lens 40 to image the living body biological sample well and accurately.
The microscope with the rotatable objective lens has a simple structure, can rotate the objective lens with multiple degrees of freedom by taking the focus as the center under the condition of ensuring that the optical path is not changed, thereby not needing to correspondingly adjust other optical paths in the microscope, effectively realizing the imaging experiment observation of the living biological sample, having low cost and being matched with a full-field or scanning microscope for use. When the microscope works, the objective lens 40 for detecting the required specification is arranged on the objective lens interface adapter 401, the scanning lens 302 of the microscope 10 images the angle scanning collimated light beam reflected by the scanning head 301 on the front focal plane thereof, then the angle scanning collimated light beam is converted by the sleeve lens 303 to converge on the back focal point thereof, then the angle scanning collimated light beam is imaged on the back focal plane thereof by the first relay lens 604, and finally the angle scanning collimated light beam is converted for the second time by the second relay lens 607 to converge on the back focal point thereof and enter the objective lens 40, in the process, the objective lens interface adapter 401 controls the first rotating frame 601 of the objective lens light path adjusting part 60 to rotate 360 degrees by taking the imaging optical axis of the microscope 10 as the rotating shaft, and simultaneously the second rotating frame 603 rotates 360 degrees by taking the horizontal optical axis after rotating 90 degrees for the first time as the rotating shaft, thereby driving the first straight angle optical adjusting frame 602 to rotate and under the action of the reflecting mirror thereon, when the invention is used for NDD detection, a dichroic mirror and a filter are placed in the inner cavity of the cage-shaped cube 608, the light beam entering the cage-shaped cube 608 assists the objective lens 40 to carry out living biological sample detection, and the photoelectric detection device 50 outputs light information to a host of the microscope 10 to control the light beam condition of the light source part 20 to meet the detection requirement, and meanwhile, the objective lens 40 controls the objective lens interface adapter 401 through the objective lens adapter 402 to drive the objective lens light path adjusting part 60 to work, so as to ensure good imaging detection observation of the living biological sample; when the invention is not used for NDD detection, a reflector is placed in the inner cavity of the cage cube 608, light beams entering the cage cube 608 assist the objective lens 40 to detect the living biological sample, and the photoelectric detection device 50 outputs light information to the host of the microscope 10 to control the light beam condition of the light source part 20 to meet the detection requirement, and meanwhile, the objective lens 40 controls the objective lens interface adapter 401 through the objective lens adapter 402 to drive the objective lens light path adjusting part 60 to work, so as to ensure good imaging detection observation of the living biological sample. Compared with the original microscope, the invention introduces a plurality of reflectors, relay lenses and other optical elements, which can widen the ultrashort laser pulse and influence the nonlinear imaging application, thus needing additional dispersion compensation.
The embodiments of the present invention have been described in detail, but the description is only for the preferred embodiments of the present invention and should not be construed as limiting the scope of the present invention. All equivalent changes and modifications made within the scope of the present invention should be covered by the present patent.
Claims (7)
1. A microscope having a rotatable objective lens, characterized by: the microscope with the rotatable objective lens comprises a microscope, a light source part, a scanning part, an objective lens, a photoelectric detection device and an objective lens light path adjusting part, wherein the light source part, the scanning part, the objective lens and the photoelectric detection device are arranged on the microscope, the scanning part enables incident light beams of the light source part to enter the objective lens light path adjusting part, and the objective lens light path adjusting part is arranged between the objective lens and the photoelectric detection device;
the scanning part comprises a scanning head, a scanning lens and a sleeve lens, wherein the scanning head is provided with a scanning reflector, and the scanning lens and the sleeve lens are sequentially arranged behind the scanning reflector;
the objective optical path adjusting part is composed of a first rotating frame, a first right-angle optical adjusting frame, a second rotating frame, a first relay lens, a second right-angle optical adjusting frame, a third right-angle optical adjusting frame, a second relay lens and a cage-shaped cube in sequence, a rotating shaft of the first rotating frame is arranged to be coincident with an imaging optical axis of the microscope, the first rotating frame is arranged on a right-angle edge of the first right-angle optical adjusting frame, the second rotating frame is arranged on the other right-angle edge of the first right-angle optical adjusting frame, a rotating shaft of the second rotating frame is arranged to pass through a focus of the objective lens, reflecting mirrors are arranged on oblique edges of the first right-angle optical adjusting frame, the second right-angle optical adjusting frame and the third right-angle optical adjusting frame, and the first relay lens and the second relay lens are arranged to be of an achromatic structure or a apochromatic structure, the upper end and the lower end of the cage-shaped cube are respectively provided with the photoelectric detection device and the objective lens;
objective pass through objective interface adapter set up in on the microscope, and still set up an objective adapter on this objective, connect the setting on the objective interface adapter first swivel mount, and this objective interface adapter sets up to electric connection the objective adapter, the objective adapter sets up to connect the cage form cube.
2. A microscope with a rotatable objective lens according to claim 1, wherein: the front focus of the first relay lens and the front focus of the second relay lens are both arranged at the rear focus of the sleeve lens.
3. A microscope with a rotatable objective lens according to claim 2, characterized in that: the transmission wavelengths of the first relay lens and the second relay lens are set to be 100nm to 2 um.
4. A microscope with a rotatable objective lens according to claim 1, wherein: the first rotating frame and the second rotating frame are both set to rotate by 360 degrees and are both manually or electrically driven.
5. A microscope with a rotatable objective lens according to claim 1, wherein: the inner cavity of the cage-shaped cube is provided with a dichroic mirror and a light filter.
6. A microscope with a rotatable objective lens according to claim 1, wherein: the inner cavity of the cage-shaped cube is provided with a reflector.
7. A microscope with a rotatable objective lens according to claim 1, wherein: the surface of the reflector is plated with a high-transmittance film, and the transmission wavelength of the reflector is set to be 100nm to 2 um.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611141424.5A CN108614351B (en) | 2016-12-12 | 2016-12-12 | Microscope with rotatable objective lens |
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| CN201611141424.5A CN108614351B (en) | 2016-12-12 | 2016-12-12 | Microscope with rotatable objective lens |
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| CN108614351A CN108614351A (en) | 2018-10-02 |
| CN108614351B true CN108614351B (en) | 2020-07-10 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112327471B (en) * | 2018-10-30 | 2022-10-14 | 深圳市真迈生物科技有限公司 | Microscope system, adjusting method thereof and sequencing system |
| CN109799222A (en) * | 2019-01-17 | 2019-05-24 | 华东师范大学 | A kind of image-pickup method of Raman face battle array EO-1 hyperion |
| CN109839732B (en) * | 2019-01-30 | 2023-10-20 | 中国科学技术大学 | Cage structure laser scanning confocal microscopic imaging system and method |
| CN113359287A (en) * | 2021-06-11 | 2021-09-07 | 江苏德恩医学技术有限公司 | Angle-adjustable rotary objective and microscope |
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| CN205942076U (en) * | 2016-07-27 | 2017-02-08 | 凝辉(天津)科技有限责任公司 | Rotatory light path of microscope objective |
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| WO2002023247A1 (en) * | 2000-09-18 | 2002-03-21 | Vincent Lauer | Confocal optical scanning device |
| CN1856725A (en) * | 2003-09-25 | 2006-11-01 | 莱卡微系统Cms有限责任公司 | Microscope with evanescent sample illumination |
| CN104541193A (en) * | 2012-07-05 | 2015-04-22 | 新加坡国立大学 | Light microscope and method of controlling the same |
| CN104634766A (en) * | 2015-01-30 | 2015-05-20 | 浙江大学 | Super-resolution device and method based on pumping-probe technology |
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