CN209178405U - A kind of device for cell endogeny electric field intervention experiment - Google Patents
A kind of device for cell endogeny electric field intervention experiment Download PDFInfo
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- CN209178405U CN209178405U CN201821802470.XU CN201821802470U CN209178405U CN 209178405 U CN209178405 U CN 209178405U CN 201821802470 U CN201821802470 U CN 201821802470U CN 209178405 U CN209178405 U CN 209178405U
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- cell
- chamber
- cell culture
- electric field
- endogeny
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Abstract
The utility model relates to a kind of devices for cell endogeny electric field intervention experiment to be put into cell climbing sheet in the sight chamber including transparent cell culture chamber, electrode assembly and DC current regulator power supply；The universal meter is connected in the culture medium of cell culture chamber；One end of the electrode assembly is set in the culture medium in cell culture room by the through-hole of cell observation cover, and the other end is arranged in buffer, and the carbon electrode connecting with DC current regulator power supply is also placed in buffer；It further include transparent sealing case, the cell culture chamber is arranged in transparent sealing case, and constent temperature heater is equipped in the transparent sealing case, and the constent temperature heater is connected with temperature controller；It further include inverted microscope, the transparent sealing case is arranged on the objective table of inverted microscope, and the object lens face sight chamber of inverted microscope, eyepiece is connected with CCD shooting len, and the CCD shooting len is connected with computer.
Description
Technical field
The utility model belongs to field of biomedicine, is related to a kind of device for cell endogeny electric field intervention experiment.
Background technique
In recent years, people gradually go deep into the research of the biological effect of endogenous electric field, are related to organizing shape in embryonic development
At the every field of the cell biologies such as, inflammatory reaction, injury repair and tumour growth, it has already been proven that the fortune of various kinds of cell
Dynamic, division is influenced by physiology endogeny electric field (EFs).One is needed to the research of physiology endogeny electric field biological effect
Device appropriate, performance should be stablized, are reproducible；It is capable of the endogenous electric field of the quasi stationary physiological strength of mould, makees for a long time
Cell for culture；And morphological observation can be carried out under the microscope.Existing device production is complicated, cannot reuse,
Power-supply device is accurate and expensive.Reference cell electrophoretic apparatus, improvement, which has made, gives external dc endogenous electricity in culture cell
The device that field is intervened can produce the direct current endogeny electric field of physiological strength, simulates interior raw endogeny electric field, acts on external training
Feeding cell studies influence of the endogenous electric field intervention to cell behavior.
Utility model content
In view of this, the purpose of this utility model is to provide a kind of dresses for cell endogeny electric field intervention experiment
It sets, reference cell electrophoretic apparatus, improves to have made and give the device of external dc endogeny electric field intervention to culture cell, it can be with
The direct current endogeny electric field of physiological strength is generated, endogenous electric field is simulated, acts on the cell of in vitro culture, studies endogenous electricity
Intervene the influence to cell behavior in field.
In order to achieve the above objectives, the utility model provides the following technical solutions:
A kind of device for cell endogeny electric field intervention experiment, including transparent cell culture chamber, set on described thin
Cell observation cover, electrode assembly and DC current regulator power supply in born of the same parents culturing room, the cell culture chamber include a pedestal and
The supporting frame being connect with the pedestal；It is equipped with a transparent sight chamber on the base, is put into cell in the sight chamber and climbs
Piece；Cell observation cover is fixedly connected with the upper end of support frame as described above body, and one is equipped at the top of the cell observation cover and is suitable for microscope
The observation port of exploring, and the cell observation cover that both ends are equipped with for the through-hole by electrode, above the sight chamber is additionally provided with company
Connect the through-hole of universal meter；The universal meter is connected in the culture medium of cell culture chamber；
The electrode assembly is U-shaped agar-salt bridge electrode, the through-hole that one end of the electrode assembly passes through cell observation cover
In the culture medium in cell culture room, other end setting is also placed with steady with direct current in buffer, in the buffer
Press the carbon electrode of stabilized current supply connection；
It further include transparent sealing case, the cell culture chamber is arranged in transparent sealing case, sets in the transparent sealing case
There is constent temperature heater, the constent temperature heater is connected with temperature controller；
It further include inverted microscope, the transparent sealing case is arranged on the objective table of inverted microscope, inverted microscope
Object lens face sight chamber, eyepiece is connected with CCD shooting len, and the CCD shooting len is connected with computer.
Further, the temperature of the constent temperature heater is maintained at 37 degrees Celsius.
Further, the DC current regulator power supply output voltage 0.1-200V exports electric current 0.01-10mA, continuously may be used
It adjusts, further includes voltage and current display and timer on the DC current regulator power supply.
Further, the cell culture chamber is made using organic glass.
Make that give external dc endogeny electric field to culture cell dry the utility model has the beneficial effects that: improving
Pre- device can produce the direct current endogeny electric field of physiological strength, simulates interior raw endogenous electric field, acts in vitro culture
Cell, and cell culture is carried out by constant incubator, experimentation is recorded by inverted microscope and CCD shooting len, is had
Conducive to completion experiment.
Detailed description of the invention
In order to keep the purpose of this utility model, technical scheme and beneficial effects clearer, the utility model provides as follows
Attached drawing is illustrated:
Fig. 1 is the DC electric field tampering devic structural schematic diagram of cultured cell in vitro described in the utility model；
Fig. 2 is the DC electric field tampering devic stereoscopic schematic diagram of cultured cell in vitro described in the utility model.
Appended drawing reference: DC current regulator power supply 1, cell culture chamber 2, cell observation cover 3, electrode assembly 4, carbon electrode
5, transparent sealing case 6, inverted microscope 7, objective table 8, eyepiece 9, object lens 10.
Specific embodiment
Below in conjunction with attached drawing, the preferred embodiment of the utility model is described in detail.
As shown in Figure 1 and Figure 2, a kind of device for cell endogeny electric field intervention experiment, including transparent cell culture
Room 2, the cell observation cover 3 on the cell culture chamber 2, electrode assembly 4 and DC current regulator power supply 1, the cell
Culturing room 2 includes a pedestal and the supporting frame connecting with the pedestal；It is equipped with a transparent sight chamber on the base, it is described
Cell climbing sheet is put into sight chamber；Cell observation cover 3 is fixedly connected with the upper end of support frame as described above body, the cell observation cover 3
Top is equipped with one and is suitable for the observation port that microscope probes, and both ends are equipped with for the through-hole by electrode, the sight chamber top
Cell observation cover 3 be additionally provided with connection universal meter through-hole；The universal meter is connected to the culture medium of cell culture chamber 2
In；
The electrode assembly 4 is U-shaped agar-salt bridge electrode, and one end of the electrode assembly 4 passes through cell observation cover 3
Through-hole be set to cell culture chamber 2 in culture medium in, the other end setting be also placed in buffer, in the buffer with directly
Flow the carbon electrode 5 that current regulator power supply 1 connects；
It further include transparent sealing case 6, the cell culture chamber 2 is arranged in transparent sealing case 6, the transparent sealing case 6
Interior to be equipped with constent temperature heater, the constent temperature heater is connected with temperature controller；
It further include inverted microscope 7, the transparent sealing case 6 is arranged on the objective table 8 of inverted microscope 7, is inverted aobvious
The 10 face sight chamber of object lens of micro mirror 7, eyepiece 9 are connected with CCD shooting len, and the CCD shooting len is connected with computer.
The temperature of the constent temperature heater is maintained at 37 degrees Celsius.
The 1 output voltage 0.1-200V of DC current regulator power supply exports electric current 0.01-10mA, is continuously adjusted, described
It further include voltage and current display and timer on DC current regulator power supply 1.
The cell culture chamber 2 is made using organic glass.
The preparation flow of observation cell includes: to take glass slide 1 (75mm × 25mm), thicken coverslip 2 (50mm ×
24mm × 0.26-0.34mm), it is pasted in parallel along long axis at a distance of 18mm on glass slide and thickeies coverslip, gone with diamond dust polishing
Extra coverslip forms a U-type groove.Put cell climbing sheet made of 18mm × 18mm coverslip when EFs intervention experiment
Enter U-type groove, then take 50mm × 25mm glass slide a piece of, be placed in U-type groove, sealed with VALAP, a rectangle sight chamber is made,
And direct current is 50mm × 18mm × 0.26- 0.34mm by acting on the space of cell, size.
The preparation flow of cell culture chamber 2 includes: to take organic glass production one internal diameter 100mm × 25mm × 15mm rectangular thin
Born of the same parents culturing room 2, bottom plate center mill out one 50mm × 20mm rectangle defect with precision milling machine.Sight chamber is pasted onto rectangle to lack
In damage, cell culture chamber 2 is made.One organic glass top cover of another system, both ends are respectively bored a hole diameter 6mm, are passed through for electrode.?
It observes above cell and bores 2 holes diameter 1.5mm at a distance of 50mm on the top cover at position, for measuring voltage in testing.Cell culture
Room 2 is cleaned after completing by cell culture materials requirement, and ethylene oxide sterilizing is stored at room temperature.
Electrode assembly 4 includes: that the electrode being connected with cell culture chamber 2 uses agar-salt bridge electrode.With length >=300mm,
Diameter 6mm glass tube high temperature plastotype charges 56 DEG C of 2% agar+Steinberg ' s solution at U-tube, cleaning and high-temperature sterilization,
Natural cooling is made.It when filling agar, avoids occurring bubble in glass tube, electrode resistance is caused to increase, influence EFs effect.It is real
When testing in the culture medium of agar-salt bridge electrode one end merging cell culture chamber 2, the other end is placed in 5 place of carbon electrode
Steinberg ' s solution connects DC power supply with conducting wire by carbon electrode 5.
DC current regulator power supply 1 includes: to design output voltage 0.1-200V according to requirement of experiment, exports electric current 0.01-
10mA is continuously adjusted, the bistable DC power supply that voltage and current is shown, Timing.In use, two output lines are connected to carbon-point
Electrode 5.
Cell culture chamber 2 is placed in 37 DEG C of cell incubator, the cell in cell culture chamber 2 and Nostoc commune Vanch bottle
Or the cell in culture dish is similar, under regular culture conditions.When no endogenous electric field is intervened, cell is made by oneself using this experiment
Culturing room 2 cultivate cell survival and keep normal physiological condition, with regular growth colouring method understand cell survival with
Cell no significant difference in Nostoc commune Vanch ware.While giving the intervention of endogenous electric field, cell culture chamber 2 is placed on dress
Have on the constant temperature inverted biologic microscope objective table 8 of transparent sealing case 6, work the temperature inside the box is kept for 37 DEG C, passes through sight chamber pair
The activity of cell is observed, and 2 small volume of cell culture chamber, observation ward is made of glass, is placed conveniently within microscope slide
On platform 8, and ideal cell image can be obtained under mirror.
Finally, it is stated that preferred embodiment above is merely intended for describing the technical solutions of the present application, but not for limiting the present application, to the greatest extent
Pipe has been described in detail the utility model by above preferred embodiment, but those skilled in the art should manage
Solution, can make various changes, without departing from the utility model claims book institute to it in the form and details
The range of restriction.
Claims (4)
1. a kind of device for cell endogeny electric field intervention experiment, it is characterised in that: including transparent cell culture chamber, set
In cell observation cover, electrode assembly and DC current regulator power supply on the cell culture chamber, the cell culture chamber includes
One pedestal and the supporting frame being connect with the pedestal；It is equipped with a transparent sight chamber on the base, is put in the sight chamber
Enter cell climbing sheet；Cell observation cover is fixedly connected with the upper end of support frame as described above body, and it is suitable that one is equipped at the top of the cell observation cover
In the observation port that microscope probes, and both ends are equipped with the cell observation cover for the through-hole by electrode, above the sight chamber
It is additionally provided with the through-hole of connection universal meter；The universal meter is connected in the culture medium of cell culture chamber；
The electrode assembly is U-shaped agar-salt bridge electrode, and one end of the electrode assembly is set to by the through-hole of cell observation cover
In culture medium in cell culture room, other end setting is also placed with steady with DC voltage-stabilizing in buffer, in the buffer
The carbon electrode of galvanic electricity source connection；
It further include transparent sealing case, the cell culture chamber is arranged in transparent sealing case, and perseverance is equipped in the transparent sealing case
Warm heater, the constent temperature heater are connected with temperature controller；
It further include inverted microscope, the transparent sealing case is arranged on the objective table of inverted microscope, the object of inverted microscope
Mirror face sight chamber, eyepiece are connected with CCD shooting len, and the CCD shooting len is connected with computer.
2. the device according to claim 1 for cell endogeny electric field intervention experiment, it is characterised in that: the constant temperature
The temperature of heater is maintained at 37 degrees Celsius.
3. the device according to claim 1 for cell endogeny electric field intervention experiment, it is characterised in that: the direct current
Current regulator power supply output voltage 0.1-200V exports electric current 0.01-10mA, is continuously adjusted, the DC current regulator power supply
On further include voltage and current display and timer.
4. the device according to claim 1 for cell endogeny electric field intervention experiment, it is characterised in that: the cell
Culturing room is made using organic glass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821802470.XU CN209178405U (en) | 2018-11-02 | 2018-11-02 | A kind of device for cell endogeny electric field intervention experiment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821802470.XU CN209178405U (en) | 2018-11-02 | 2018-11-02 | A kind of device for cell endogeny electric field intervention experiment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209178405U true CN209178405U (en) | 2019-07-30 |
Family
ID=67363010
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821802470.XU Expired - Fee Related CN209178405U (en) | 2018-11-02 | 2018-11-02 | A kind of device for cell endogeny electric field intervention experiment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209178405U (en) |
-
2018
- 2018-11-02 CN CN201821802470.XU patent/CN209178405U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190730 Termination date: 20201102 |