CN209974799U

CN209974799U - Stem cell constant temperature culture apparatus

Info

Publication number: CN209974799U
Application number: CN201920578564.1U
Authority: CN
Inventors: 刘长征
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-04-25
Filing date: 2019-04-25
Publication date: 2020-01-21
Anticipated expiration: 2029-04-25

Abstract

The utility model discloses a stem cell constant temperature culture apparatus, including the incubator, the bottom inner wall of incubator is provided with four evenly distributed's seat shell, and the side outer wall of seat shell has all opened the louvre, the side inner wall of seat shell is provided with the support shell, and the bottom inner wall of support shell has placed the cultivation box, the pit seat has been placed to the bottom inner wall of cultivation box, and the top outer wall of pit seat opens there is the pit, the bottom inner wall of seat shell is provided with the electric plate, and the bottom outer wall of support shell is provided with temperature sensor, the top of incubator is provided with the case lid, and the top outer wall of case lid opens there are four evenly distributed's handle hole. The utility model discloses can control stem cell's culture temperature, heating efficiency is high, makes the culture medium can rapid heating up to required temperature, can carry out multiple stem cell's cultivation simultaneously, and the baffle can prevent to influence each other between each region, can prevent that external bacterium from getting into in the incubator.

Description

Stem cell constant temperature culture apparatus

Technical Field

The utility model relates to a stem cell culture technical field especially relates to a stem cell constant temperature culture apparatus.

Background

The neural stem cell is a kind of stem cell existing in embryonic or adult brain, spinal cord and other tissues, is a kind of mother cell with division potential and self-renewal capacity, can be differentiated into various cells of nerve tissues such as neuron, astrocyte, oligodendrocyte and the like through unequal division modes, and can also be trans-differentiated into blood cells and skeletal muscle cells, and the current scientific technology can be used for culturing and amplifying the neural stem cell in vitro.

Through search, the chinese patent application No. CN201721041909.7 discloses a neural stem cell culture device, which includes a culture container. One of the neural stem cell culture devices in the above patents has the following disadvantages:

1. this culture apparatus can only detect the temperature during stem cell culture and cannot adjust the temperature during stem cell culture.

2. The culture device can only culture one type of stem cells at a time, and the culture efficiency is low.

3. When the operating bottle cap is opened to operate the culture device, external bacteria easily enter the culture device from the operating hole, and stem cell pollution is caused.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects existing in the prior art and providing a stem cell constant temperature culture device.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a stem cell constant-temperature culture device comprises an incubator, wherein four seat shells are uniformly distributed on the inner wall of the bottom of the incubator, radiating holes are formed in the outer walls of the side surfaces of the seat shells, a supporting shell is arranged on the inner wall of the side surface of the seat shell, a culture box is placed on the inner wall of the bottom of the supporting shell, a pit seat is placed on the inner wall of the bottom of the culture box, a pit is formed in the outer wall of the top of the pit seat, an electric heating plate is arranged on the inner wall of the bottom of the seat shell, a temperature sensor is arranged on the outer wall of the bottom of the supporting shell, a box cover is arranged above the incubator, four operation holes are uniformly distributed on the outer wall of the top of the box cover, a sealing gasket is arranged on the inner wall of each operation hole, an end cover is bonded on the outer wall of the top of the sealing gasket, a handle is arranged on the outer wall of, the inside joint of incubator has the baffle, and the bottom outer wall and the top outer wall of baffle all bond there is the rubber pad.

Further, four side inner walls of incubator all are provided with the lamp stand, and the equal joint of inner wall of lamp stand has sterilamp.

Further, the outer wall of one side of the case lid relative all is provided with the buckle, and the outer wall top of one side of the incubator relative all is provided with the dop, and buckle and dop looks adaptation.

Further, the outer wall of the top of the box cover is provided with a lifting handle, and the outer wall of the lifting handle is sleeved with a rubber sleeve.

Furthermore, the vertical section of the partition board is of a cross-shaped structure.

Further, the pits are of a hemispherical structure.

Furthermore, the electric hot plate and the ultraviolet germicidal lamp are both connected with a switch, the switch is connected with a processor, and the signal output end of the temperature sensor is connected with the signal input end of the processor through a signal line.

The utility model has the advantages that:

1. through setting up electric plate and temperature sensor, set up electric plate and temperature sensor in the seat shell, can heat the culture box through electric plate and temperature sensor, then can control stem cell's cultivation temperature, heating efficiency is high, makes the culture medium ability rapid heating up to required temperature.

2. Through setting up baffle and four cultivation boxes, set up the baffle in the incubator, and the cross section of baffle is cruciform structure, and the baffle falls into four regions with the incubator, and all sets up a cultivation box in every region, then can carry out the cultivation of multiple stem cell simultaneously, and the baffle can prevent to influence each other between each region.

3. Through setting up the silica gel film, set up the silica gel film in the handle hole, and be provided with the round hole on the silica gel film, at the stem cell culture in-process, when needs add nutrient solution or inhibitor, the outer wall of burette can contact with the outer wall of round hole, can prevent then that external bacterium from getting into the incubator.

Drawings

FIG. 1 is a schematic structural view of a stem cell constant temperature culture apparatus provided by the present invention;

FIG. 2 is a schematic view of the external structure of a stem cell constant temperature culture apparatus according to the present invention;

FIG. 3 is a schematic view of the incubator structure of the stem cell constant temperature culture apparatus of the present invention;

FIG. 4 is a schematic diagram of the circuit connection of the stem cell constant temperature culture apparatus of the present invention.

In the figure: 1-incubator, 2-pit seat, 3-clapboard, 4-rubber pad, 5-pit, 6-temperature sensor, 7-electric hot plate, 8-heat dissipation hole, 9-seat shell, 10-incubator, 11-ultraviolet germicidal lamp, 12-chuck, 13-buckle, 14-case cover, 15-sealing gasket, 16-handle, 17-silica gel film, 18-handle, 19-operation hole, 20-round hole, 21-end cover, 22-observation window.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Referring to fig. 1-4, a stem cell constant temperature culture device, comprising an incubator 1, the bottom inner wall of the incubator 1 is connected with four seat shells 9 uniformly distributed through screws, and the side outer walls of the seat shells 9 are all opened with heat dissipation holes 8, the side inner walls of the seat shells 9 are welded with support shells 23, and the bottom inner wall of the support shells 23 is placed with a culture box 10, the bottom inner wall of the culture box 10 is placed with a concave seat 2, and the top outer wall of the concave seat 2 is opened with a concave pit 5, the bottom inner wall of the seat shells 9 is connected with an electric heating plate 7 through screws, and the bottom outer wall of the support shells 23 is connected with a temperature sensor 6 through screws, a box cover 14 is arranged above the incubator 1, and the top outer wall of the box cover 14 is opened with four operation holes 19 uniformly distributed, the inner wall of the operation holes 19 is inserted with a sealing gasket 15, and the top outer wall of the sealing gasket 15 is bonded, the silica gel film 17 is bonded to the bottom of the inner wall of the side face of the operation hole 19, the round hole 20 is formed in the outer wall of the bottom of the silica gel film 17, the partition plate 3 is clamped inside the incubator 1, and the rubber pad 4 is bonded to the outer wall of the bottom and the outer wall of the top of the partition plate 3.

The utility model discloses in, four side inner walls of incubator 1 all have the lamp stand through the screw connection, and the equal joint of inner wall of lamp stand has sterilamp 11, the relative one side outer wall of case lid 14 all has buckle 13 through the screw connection, relative one side outer wall top of incubator 1 all has dop 12 through the screw connection, and buckle 13 and dop 12 looks adaptation, the top outer wall welding of case lid 14 has handle 18, and the outer wall of handle 18 has cup jointed the rubber sleeve, baffle 3's perpendicular cross-section is cruciform structure, pit 5 is hemispherical structure, electric plate 7 and sterilamp 11 all are connected with the switch, and the switch is connected with the treater, temperature sensor 6's signal output part passing signal line connects in the signal input part of treater, the model of treater is ARM9TDMI, temperature sensor 6's model is BM 100.

The working principle is as follows: when in use, culture media required by stem cell culture are respectively added into the culture boxes 10, the box cover 14 is covered, the ultraviolet germicidal lamp 11 is used for sterilizing the interior of the culture device and the culture media, after sterilization, the end cover 21 is opened through the handle 16, stem cells are dripped into each culture box 10 from the operation hole 19 through the dropper, the temperature of each interval in the culture box 1 can be controlled through the electric heating plate 7 and the temperature sensor 6, the temperature is suitable for the culture of the stem cells, the partition plate 3 can prevent the temperature of each interval from influencing each other, when the stem cells proliferate, the pits 5 on the pit seat 2 can enable the stem cells to slightly gather in each pit at the bottom of the container, thereby preventing the stem cells from gathering in large quantity, when nutrient solution or inhibitor is required to be added into the culture media in the culture process, the end cover 21 can be opened, the dropper is inserted into the round hole 20 for adding, the silica gel film 17 can prevent external bacteria from entering the culture box 1, the worker can observe the culture condition of the stem cells through the observation window 22.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. The stem cell constant-temperature culture device comprises an incubator (1) and is characterized in that four seat shells (9) which are uniformly distributed are arranged on the inner wall of the bottom of the incubator (1), radiating holes (8) are formed in the outer walls of the side faces of the seat shells (9), a supporting shell (23) is arranged on the inner wall of the side face of the seat shell (9), a culture box (10) is placed on the inner wall of the bottom of the supporting shell (23), a pit seat (2) is placed on the inner wall of the bottom of the culture box (10), pits (5) are formed in the outer wall of the top of the pit seat (2), an electric heating plate (7) is arranged on the inner wall of the bottom of the seat shell (9), a temperature sensor (6) is arranged on the outer wall of the bottom of the supporting shell (23), a box cover (14) is arranged above the incubator (1), and four operation holes (19) which are uniformly distributed are formed in, the inner wall of handle hole (19) is provided with sealed pad (15), and the top outer wall that seals up pad (15) bonds and have end cover (21), and the top outer wall of end cover (21) is provided with handle (16), the side inner wall bottom bonding of handle hole (19) has silica gel film (17), and the bottom outer wall of silica gel film (17) opens and have round hole (20), the inside joint of incubator (1) has baffle (3), and the bottom outer wall and the top outer wall of baffle (3) all bond and have rubber pad (4).

2. The stem cell constant-temperature culture device as claimed in claim 1, wherein lamp holders are arranged on the inner walls of four sides of the culture box (1), and ultraviolet germicidal lamps (11) are clamped on the inner walls of the lamp holders.

3. The stem cell constant-temperature culture device as claimed in claim 2, wherein the outer wall of the box cover (14) on the opposite side is provided with a buckle (13), the top of the outer wall of the culture box (1) on the opposite side is provided with a clamping head (12), and the buckle (13) is matched with the clamping head (12).

4. The stem cell constant-temperature culture device as claimed in claim 3, characterized in that a handle (18) is arranged on the outer wall of the top of the box cover (14), and a rubber sleeve is sleeved on the outer wall of the handle (18).

5. A stem cell thermostatic culture device according to claim 4, characterized in that the vertical section of the partition plate (3) is a cross-shaped structure.

6. A stem cell thermostatic culture device according to claim 5, wherein the concave pit (5) is a hemispherical structure.

7. The stem cell constant-temperature culture device as claimed in claim 6, characterized in that the electric heating plate (7) and the ultraviolet germicidal lamp (11) are both connected with a switch, the switch is connected with a processor, and the signal output end of the temperature sensor (6) is connected with the signal input end of the processor through a signal wire.