CN208984507U - A kind of flow cytometer cell filter of convenient and efficient - Google Patents
A kind of flow cytometer cell filter of convenient and efficient Download PDFInfo
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- CN208984507U CN208984507U CN201821654422.0U CN201821654422U CN208984507U CN 208984507 U CN208984507 U CN 208984507U CN 201821654422 U CN201821654422 U CN 201821654422U CN 208984507 U CN208984507 U CN 208984507U
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- strainer
- cylinder
- flow cytometer
- retainer plate
- convenient
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Abstract
A kind of flow cytometer cell filter of convenient and efficient, is made of cylinder 1, pushing mechanism 2, strainer 3, strainer retainer plate 4, limiter 5；Pushing mechanism 2 is provided in cylinder 1,1 lower end outer wall of cylinder is cased with strainer retainer plate 4, and strainer 3 is set to 1 lower end of cylinder by strainer retainer plate 4.The utility model relates to flow cytometer cell filter it is easy to operate, sample and reagent loss are few；Maxicell and impurity blocking sieve in cell separation process can also be avoided to influence filter efficiency, the quick separating of cell during sample-adding is realized, improve the efficiency of sample preparation.
Description
Technical field
The utility model relates to a kind of flow cytometer cell filters of convenient and efficient, belong to medical apparatus technology neck
Domain.
Background technique
Flow cytometer is a kind of hygiene medical treatment instrument, be mainly used for in cell suspension cell or organelle carry out it is fast
Speed measurement, there is very extensive purposes in fields such as medicine, biology and agronomy.Flow cytometer belongs to high-end, accurate, valuable
Instrument, so the pre-processing requirements to sample are also relatively high.Before loading, either culture cell or the histocyte of processing will
It is prepared into cell suspension, with filter screen filtration, in case packed cell or impurity block instrument.
The cell filter for being traditionally used for flow cytometer is that one piece of nylon wire plug is temporarily taken to carry out in detection pipe
Filter, it is time-consuming and laborious, influence working efficiency.For this traditional filter type, people are improved, for example, utility model
Patent " a kind of flow cytometer cell filter " (patent No.: 201320742579X), involved in the utility model patent
Cell filter can be placed directly in detection pipe, and 10 filters link together, and can be used in combination but also independent
It uses, facilitates the large batch of sample of processing.But the technical solution still has technological deficiency: 1. are due to the cell filter
It is filtered by the effect of gravity, can cause to filter because of entrapped cell and the accumulated plugging strainer of impurity during the filtration process
The problem of efficiency decline and sample losses；2. above-mentioned cell filter is placed directly in detection pipe and operates, due to filtering
The difference of external and internal pressure in the process will lead to filter efficiency and further decline.
To sum up, the problem of the generally existing inefficient filtering of the existing cell filter dedicated for flow cytometer, therefore,
There is an urgent need to a kind of new devices, to solve the above problems.
Utility model content
The utility model provides a kind of flow cytometer cell filter that can be solved the above problems, i.e. the utility model institute
What is be related to is simple in the cell filter structure of flow cytometer, easy to use, additionally it is possible to overcome it is existing in the prior art under
State problem: strainer is easy to the accumulation because of impurity or cell due to strainer filtering effective area is made to become smaller during 1. cell filtrations,
Aim cell is difficult to act through strainer by gravity again, to influence the efficiency of sample preparation；2. existing filter according to
It is filtered by gravity, detection inner air tube will form draught head if not circulating with outside air, will affect filtering effect
Rate.
The purpose of the utility model is to provide a kind of flow cytometer cell filter of convenient and efficient, the fluidic cells
Instrument cell filter is made of cylinder 1, pushing mechanism 2, strainer 3, strainer retainer plate 4, limiter 5；It is provided with and pushes away in cylinder 1
Dynamic component 2, pushing mechanism 2 include push rod 201 and piston 202, and 201 lower end of push rod is fixedly connected with piston 202, outside push rod 201
It is exposed to 1 upper end of cylinder, is difficult in aim cell again by gravity when acting through strainer needs by external force, by pushing away
Bar 201 accelerates filter efficiency, avoids making strainer filtering significant surface because of the accumulation of impurity or maxicell during cell filtration
Product becomes smaller, and the purpose that cell efficiently separates is not achieved.Strainer retainer plate 4 is hollow cylinder, and 4 outer wall of strainer retainer plate is provided with
Several fins guarantee that detection pipe and outside air circulate, thereby improving filtration efficiency.1 lower end of cylinder and strainer retainer plate 4 are removable
Connection is unloaded, the diameter of strainer 3 is greater than the internal diameter of strainer retainer plate 4, and strainer 3 is fixed on the bottom of cylinder 1 by strainer retainer plate 4
Portion can disassemble strainer retainer plate 4 from 1 lower end of cylinder after completing filter operation every time, replace strainer 3.
Further, 1 lower end outer wall of cylinder is provided with external screw thread, and 4 inner wall of strainer retainer plate is provided with internal screw thread, cylinder 1
Lower end is connect with 4 spiral of strainer retainer plate, and 1 lower end of cylinder is facilitated to connect with strainer retainer plate 4, guarantees that cylinder 1 is fixed with strainer
The closely connection of circle 4.
Further, several fin longitudinal arrangements setting of 4 outer wall of strainer retainer plate, guarantees detection inner air tube and the external world
Air circulation prevents draught head, influences the filter efficiency of cell.
Further, 1 inner wall of cylinder is provided with limiter 5, prevents from excessively pushing push rod 201, touches piston 202
Filtered fluid leads to the loss and pollution of cell liquid.
Further, the bottom section of 1 inner wall of cylinder is arranged in limiter 5, guarantees that piston 202 does not reach filtered fluid
Pushing mechanism 2 can be made to help aim cell filtering simultaneously.
Further, 1 outer wall of cylinder is equipped with holding part 101, convenient for users to operating cell filter.
Further, holding part 101 is set to the middle part or top of cylinder 1, can be with uniform speed convenient for operator
Degree pushes push rod.
The utility model has the beneficial effects that
(1) the utility model relates to flow cytometer cell filter structure it is simple, there is practicability, operability.
(2) the utility model relates to flow cytometer cell filter can directly be placed in detection pipe, strainer is fixed
The fin of ring outer wall setting can guarantee that detection pipe inner air and outer air circulates, and air pressure is consistent, improve the filter efficiency of cell filter,
Reduce the loss of sample and reagent.
(3) when strainer makes strainer filtering effective area become smaller because of the accumulation of impurity or cell, filtering rate reduces, benefit
With pushing mechanism, achieve the purpose that cell efficiently separates, improves the efficiency of sample preparation.
(4) the utility model relates to flow cytometer cell filter can be used for the mistake of aim cell in supernatant liquid
Filter removes pushing mechanism, in the case where inside and outside detection pipe without pressure difference, is filtered by the effect of gravity.
(5) it can prevent from excessively pushing push rod, make piston contact to filtered fluid, lead to the loss and pollution of cell liquid, guarantee
Pushing mechanism can be made to help aim cell filtering while piston contact is less than filtered fluid.
Detailed description of the invention
FIG. 1 is a schematic structural view of the utility model
1- cylinder, 101- holding part, 2- pushing mechanism, 201- push rod, 202- piston, 4- strainer retainer plate, 5- limit
Device
Fig. 2 is that the utility model uses schematic diagram
1- cylinder, 2- pushing mechanism, 4- strainer retainer plate, 5- limiter, 6- detection pipe
Fig. 3 is the structural schematic diagram of the utility model strainer retainer plate
1- cylinder, 4- strainer retainer plate
Fig. 4 is the structural schematic diagram of the fixed strainer of the utility model strainer retainer plate
1- cylinder, 3- strainer, 4- strainer retainer plate
Specific embodiment
The utility model is further described through with reference to the accompanying drawings and detailed description.
A kind of flow cytometer cell filter of the convenient and efficient of embodiment 1
A kind of flow cytometer cell filter of convenient and efficient is by cylinder 1, pushing mechanism 2, strainer 3, strainer retainer plate
4, limiter 5 forms；Pushing mechanism 2 is provided in cylinder 1, cylinder 1 is equipped with holding part 101, and holding part 101 is set to
The middle part or top of cylinder 1；Pushing mechanism 2 includes push rod 201 and piston 202, and 201 lower end of push rod is fixedly connected with piston 202；
Push rod 201 exposes to 1 upper end of cylinder, and 1 lower end outer wall of cylinder is provided with external screw thread, and strainer retainer plate 4 is provided with internal screw thread, cylinder
1 lower end is connect with 4 spiral of strainer retainer plate, and the diameter of strainer 3 is greater than the internal diameter of strainer retainer plate 4, and strainer 3 is fixed by strainer
Circle 4 is fixed on 1 bottom of cylinder, and the bottom section of 1 inner wall of cylinder is arranged in limiter 5.
A kind of application method of the convenient and efficient flow cytometer cell filter of embodiment 2
In use, flow cytometer cell filter is placed in detection pipe by the way that strainer retainer plate is stable, will prepare
Good cell suspending liquid injects cylinder, stands half a minute, is filtered by gravity, then pushes push rod, cell is rapidly completed
Lock out operation.
Claims (7)
1. a kind of flow cytometer cell filter of convenient and efficient, which is characterized in that the flow cytometer cell filter,
It is made of cylinder (1), pushing mechanism (2), strainer (3), strainer retainer plate (4), limiter (5)；Promotion is provided in cylinder (1)
Component (2), pushing mechanism (2) include push rod (201) and piston (202), and push rod (201) lower end is fixedly connected with piston (202)；
Push rod (201) exposes to cylinder (1) upper end, and strainer retainer plate (4) is hollow cylinder, and the outer wall of strainer retainer plate (4) is arranged
There are several fins；Cylinder (1) lower end is detachably connected with strainer retainer plate (4), and the diameter of strainer (3) is greater than strainer retainer plate
(4) internal diameter, strainer (3) are fixed on the lower end base of cylinder (1) by strainer retainer plate (4).
2. a kind of flow cytometer cell filter of convenient and efficient according to claim 1, which is characterized in that the cylinder
Body (1) lower end outer wall is provided with external screw thread, and strainer retainer plate (4) inner wall is provided with internal screw thread, and cylinder (1) lower end is fixed with strainer
Enclose the connection of (4) spiral.
3. a kind of flow cytometer cell filter of convenient and efficient according to claim 1, which is characterized in that the filter
Several fin longitudinal arrangements of net retainer plate (4) outer wall are arranged.
4. a kind of flow cytometer cell filter of convenient and efficient according to claim 1, which is characterized in that cylinder
(1) inner wall is provided with limiter (5).
5. a kind of flow cytometer cell filter of convenient and efficient according to claim 4, which is characterized in that limiter
(5) bottom section in cylinder (1) inner wall is set.
6. a kind of flow cytometer cell filter of convenient and efficient according to claim 1, which is characterized in that the cylinder
Body (1) outer wall is equipped with holding part (101).
7. a kind of flow cytometer cell filter of convenient and efficient according to claim 6, which is characterized in that described to grab
Hold middle part or top that component (101) is set to cylinder (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821654422.0U CN208984507U (en) | 2018-10-12 | 2018-10-12 | A kind of flow cytometer cell filter of convenient and efficient |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821654422.0U CN208984507U (en) | 2018-10-12 | 2018-10-12 | A kind of flow cytometer cell filter of convenient and efficient |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208984507U true CN208984507U (en) | 2019-06-14 |
Family
ID=66787250
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821654422.0U Active CN208984507U (en) | 2018-10-12 | 2018-10-12 | A kind of flow cytometer cell filter of convenient and efficient |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208984507U (en) |
-
2018
- 2018-10-12 CN CN201821654422.0U patent/CN208984507U/en active Active
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