CN208924838U - A kind of Spawn incubation bag of the fungi-proofing function of tool ventilation - Google Patents

Abstract

The utility model relates to a kind of Spawn incubation bags of the fungi-proofing function of tool ventilation, belong to edible mushroom technical field.The Spawn incubation bag is made of Spawn incubation bag body and fungi-proofing cloth patch；The Spawn incubation bag is made of Spawn incubation bag body and fungi-proofing cloth patch；There is sack in Spawn incubation bag body, fungi-proofing cloth patch is sticked to below sack from the position 160~200mm of sealing part, while wiping out the pocket portion of fungi-proofing cloth patch inside.Fungi-proofing cloth patch aperture is 0.01~0.05um.The edible mushroom seed in container can be made to obtain sufficient clean air while growing bacterium germination using Spawn incubation bag, fungi-proofing cloth pastes and can effectively exclude the various microorganisms in air, it is ensured that the strain of multiple eating bacterium kind is grown safely in a reservoir.

Description

A kind of Spawn incubation bag of the fungi-proofing function of tool ventilation

Technical field:

The utility model relates to a kind of Spawn incubation bags of the fungi-proofing function of tool ventilation, belong to technical field of edible fungi cultivation.

Background technique

Edible mushroom has high protein, low fat, contains a variety of amino acid of needed by human body and microelement, and have numerous food product The health-care effect that can not replace, it has also become 21 century health type food.As people's living standard improves, edible mushroom consumption figure Every year with 7% speed sustainable growth.In the China for possessing 1,300,000,000 populations, edible bacterium amount is consumed year up to more than 3,000 ten thousand tons.Add Edible mushroom be to be produced using agriculture and forestry organic waste material as culture medium, meet the 3R (Reduce that international community is advocated Minimizing, Reuse are recycled, Recycle recycling) index system, be conducive to the benign cycle for constructing Agro-ecological System, And promote lasting, harmonious, the sound development of ecological environment.China is a large agricultural country, and agriculture and forestry crop waste resource is abundant, greatly Most stalk material becomes environomental pollution source after burning, it is empty that stalk can also generate a large amount of carbon dioxide pollution in spontaneous fermentation Gas.And the macromoleculars nutrients such as cellulose and sugar in stalk material can be decomposed and be absorbed to the mycelia of edible mushroom especially agaricus bisporus Matter converts stalk material to the food of fungi of high protein, makes major contribution for human survival and health, promotes rural economy Healthy and sustainable development realizes the two-win of environmental protection and economic development.

Edible fungus culturing matrix nutrition is abundant, and especially culture medium quick-acting nutrition after high-temperature sterilization increases, and is meeting Mycelia is also more subject to various miscellaneous bacterias and worm mite infringement while growth, China's mushroom industry is in the course of cultivation by various Up to 15-20%, the control polluted in growth program is the core of entire growth for the underproduction caused by disease pest endangers, Annual strain enterprise has 10% or more strain to be infected and scrap by various insect pests and germ.Although in growth Pollution prevention program is designed in each link, from strain substrate container selection, matrix sterilizing, transfer room air cleaning require to Various prevention and control measures is made in air cleaning requirement of bacteria-producing room etc., but has and do not discharge place, and pollution still has.Through life The careful investigation of stages of labor sequence is analyzed, it is believed that the bacterium germination stage of the most of places of pollution source after inoculation, because the container of strain is all Equipped with an inoculation mouth, the air filtration gasket hole in the bottle cap of inoculation mouth is excessive, is easy to bring disease pest into air exchange Source and cause strain pollution.Edible fungus species manufacturing enterprise in China's uses glass in original seed and cultivar production process at present Bottle, plastic bottle, polybag make container, and bottleneck or sack are sealed using plastic closure, and layer of non-woven fabric is added in plastics upper shield As the barrier of air filtration and barrier miscellaneous bacteria, but non-woven fabrics aperture, in 50-100um, the air hole of large aperture keeps air logical Freely, but miscellaneous bacteria and pest also free in and out therewith, and therefore, frequent occurrence, acarid and bacterium mosquito also often appear in bag to living contaminants Interior feeding mycelia, causes insect pest and miscellaneous bacteria to break out, and strain pollution, bacteria environmental degradation, a large amount of strains are scrapped when serious, and strain is raw It produces and stops rectifying or close down.How to solve by the living contaminants that bottle/bag mouth imprecision causes to be current edible fungus species industry face The outstanding problem faced, it is the important leverage of strain safety in production that searching, which can ventilate and the Novel antibacterial cloth of disease pest can be stopped to paste,.

Summary of the invention

Technical problem

The technical problem to be solved by the utility model is to provide a kind of edible fungi strain bag of the fungi-proofing function of tool ventilation, solutions The low problem of pollution rate high finished product rate present in certainly existing edible fungus species production bacterium germination.

Technical solution

The Spawn incubation bag is made of Spawn incubation bag body (1) and fungi-proofing cloth patch (2)；Spawn incubation bag body has on (1) Sack (3), fungi-proofing cloth patch (2) are sticked to below sack (3) from the position 160~200mm of sealing part, while wiping out in fungi-proofing cloth patch The pocket portion of side, the fungi-proofing cloth patch aperture is 0.01~0.05um.Fungi-proofing cloth pastes long 110mm × wide 60mm, number cells Up to 5600.

The fungi-proofing cloth patch is overlapped by non-woven fabrics with microporous teflon membran suppresses and integral in a mold, using surpassing Sound wave determines aperture, and adjusting fungi-proofing cloth patch aperture is 0.01~0.05um；The microporous teflon membran is by polytetrafluoroethyl-ne Olefine resin is made, and membrane aperture is required in 0.01-0.05um, 50-80um of thickness, and porosity up to 80%, select by the non-woven fabrics The ES hot rolling type non-woven fabrics of changzhou Han Ke non-woven fabrics Co., Ltd production, with a thickness of 50g/m²。

The Spawn incubation bag body (1) is a kind of polybag for having transparent high temperature resistant high-pressure polypropylene CPP film, according to life Produce the formula and matrix weight of parent species, strain and cultivar, respectively three kinds of bacterium bag specifications, parent species bacterium bag specification are as follows: long 300 × wide by 180 × thickness 0.07mm；Original seed bacterium bag specification: long 350 × wide by 220 × thickness 0.07mm；Cultivar bacterium bag specification are as follows: long 450 × wide by 300 × thickness 0.07mm.

Beneficial effect

1, the utility model has done many screening tests, the high-pressure polypropylene preferably gone out in the selection of strain container first CPP film is as strain plastic bag container.For this film by the production and sales of Zhejiang Zhong Chengsu industry Co., Ltd, the name of an article is 5 layers of high temperature resistant total Synthesis film is squeezed, this film can be packed into various edible fungi substrates (grain, cotton seed hulls, sawdust, corncob, wheat as Spawn incubation bag Grain, fermentation material etc.), capacity is up to 0.5-10.0kg；This type of strain bag compares the strain bag thickness increase by 50% of other types, but It is more soft than other strain bags, sack can be repeatedly overturn after sealing and carries out shaking kind, and the plastics of strain bag not still may be used by brittle do not crack Keep original shape；Strain bag transparency height is clearly viewed mycelia growth, miscellaneous bacteria in bag in bag external enwergy and breeds situation；Plastics kind Bag high temperature resistant can be for 5 hours in 125 DEG C of sterilizing cabinets of temperature above non-sclerous non-fusible, and indices, which reach strain bag, to be managed The performance indicator thought.

2, the utility model show that 4 data of table, test result are shown with 0.01-by multiple batches of screening test The indices of the mushroom of the fungi-proofing cloth patch strain bag culture in the aperture 0.05um are normal, and aeration is preferable in bacterium bag, does not have There is the flatulence phenomenon of bacterium bag；Water-in-bag part is sufficient, and fungi-proofing cloth patch window culture medium moisture content is maintained, and mycelia growth is normal； Especially miscellaneous bacteria amount significantly reduces during cultivating in a fungus bag, and kind bag yield rate is increased to 99.6%, stable product quality.Pass through 2 Multiple batches of test more than year is determined using the fungi-proofing cloth patch in the 0.01-aperture 0.05um as edible fungus species growbag.According to edible The oxygen that can only be allowed in air and carbon dioxide set needed for air in the growth of bacterium mycelia is swapped by micropore, and benefit Entered with micropore barrier multiple-microorganism, fungi-proofing cloth pastes long 110mm × wide 60mm, number cells up to more than 5600, be able to satisfy in Oxygen exchange amount needed for the mycelia growth of multiple eating bacterium strain.

3, the fungi-proofing cloth patch quality being fabricated to by polytetrafluoroethylene film is frivolous, and performance is stablized, and high temperature high voltage resistant is subjected to 125 DEG C 5 small timeinvariances of high temperature, micropore do not reduce, improve one toward it is used with low pressure polyethylene microporous barrier non-refractory and The shortcomings that pore membrane reduces (micropore is by polytetrafluoroethylene (PTFE) film production in the prior art).Therefore, there is hole using this fungi-proofing cloth patch Diameter constant magnitude, the strong advantage of fungi-proofing performance.

By largely screening, the ES hot rolling type non-woven fabrics of changzhou Han Ke non-woven fabrics Co., Ltd production, thickness are selected For 50g/m², such non-woven fabrics 5 hours under 125 DEG C high temperature are non-fusible, not modification, can fuse to be formed with polypropylene plastics pocket Closed closing line, ES hot rolling type non-woven fabrics have preferable gas permeability, water repellency and bullet drawing property.

4, more than 2 years come application fungi-proofing cloth patch strain bag do container production dictyophora phalloidea, coprinus comatus, Stropharia rugoso-annulata, agaricus bisporus, The mycelia such as Brazilian mushroom, mushroom, Pleurotus ferulae, grifola frondosus and Phellinus grow more slow edible fungus variety and its strain production In, it is shown from table 5, mycelia fast growing, mycelia is not agglomerated in bag, and hand is grabbed fungus block and easily dispersed, and mushroom fragrance is dense, culture Time shortens obviously, and strain yield rate significantly improves.The mycelia purseful time 30 days or so, 0.6% or less contamination rate；Mould Pollution rate 0.3%；Yeast pollution rate 0%；Acarid growth rate 0%；99% or more bag yield rate of kind.

Detailed description of the invention

Fig. 1 is parent species Spawn incubation bag structural schematic diagram

Fig. 2 is original seed Spawn incubation bag structural schematic diagram

Fig. 3 is cultivar original seed Spawn incubation bag structural schematic diagram

The fungi-proofing cloth of 1- Spawn incubation bag donor 2- pastes 3- Spawn incubation bag sack

Specific embodiment

The utility model will be further described with reference to the accompanying drawing.

The Spawn incubation bag is made of Spawn incubation bag body (1) and fungi-proofing cloth patch (2)；Spawn incubation bag body has on (1) Sack (3), fungi-proofing cloth patch (2) are sticked to below sack (3) from the position 160~200mm of sealing part, while wiping out in fungi-proofing cloth patch The pocket portion of side.Fungi-proofing cloth patch aperture is 0.01~0.05um.

Spawn incubation bag body (1) is a kind of polybag for having transparent high temperature resistant high-pressure polypropylene CPP film, female according to production The formula and matrix weight of kind, strain and cultivar make 3 kinds of strain bag specifications, parent species bacterium bag specification respectively are as follows: long 300 × wide by 180 × thickness 0.07mm, charging weight are 0.8 kilogram；Original seed strain bag specification: long 350 × wide by 220 × thickness 0.07mm, charging Weight is 1.5 kilograms；Cultivar strain bag specification are as follows: long 450 × wide by 300 × thickness 0.07mm, charging weight are 2.5 kilograms.

1, Spawn incubation bag makes

The utility model has done many screening tests in the selection of strain container, from vial, plastic bottle to polybag Charging production of hybrid seeds test has all been carried out, has observed bacterium germination and living contaminants situation, it is intended to therefrom select the bacterium for being adapted to growth Kind of packing container, and cooperate to make plastics kind that the strain for being suitable for a variety of kinds produces and corresponding to plastic products producer Strain bag specification.It is shown in Table 1, by testing the high-pressure polypropylene CPP film conduct preferably gone out from the strain container of 6 seed types Strain plastic bag container.For this film by the production and sales of Zhejiang Zhong Chengsu industry Co., Ltd, the name of an article is that 5 layers of co-extrusion of high temperature resistant synthesize film, This film can be packed into various edible fungi substrates (grain, cotton seed hulls, sawdust, corncob, wheat, fermentation material) as Spawn incubation bag, Capacity is up to 0.5-10.0kg；This type of strain bag increases by 50% compared to the strain bag thickness of other types, but more soft than other strain bags It is soft, sack can be repeatedly overturn after sealing and carries out shaking kind, and not brittle do not crack of the plastics of strain bag can still keep original shape；Bacterium Kind bag transparency height is clearly observed mycelia growth, miscellaneous bacteria in bag in bag external enwergy and breeds situation；Plastics kind bag high temperature resistant can be For 5 hours above non-sclerous non-fusible in 125 DEG C of sterilizing cabinets of temperature, indices reach the ideal performance of strain bag and refer to Mark.

1 strain bottle/bag type of table is compared with performance

2, fungi-proofing cloth patch aperture determines and makes

The existing seed bottle in China or plastic bag for cultivation of mushroom are using bottleneck or sack as charging, inoculation, closing and to breathe freely Used, closing and breathing freely is the both sides of conflict, therefore, is often able to gas permeability in practice and just has no time to attend to close Property, vice versa.So the pollution of miscellaneous bacteria and pest in strain production happens occasionally, closed for preferably solution sack and logical The contradiction of gas, feed inlet and outlet and blow vent, which are provided separately, can efficiently solve ventilation and closed contradiction, i.e., in polybag Designated position opens a hole by Air permenbility size and pastes upper ventilated membrane at aperture position again, and being exclusively used in air when growth needs It wants, plastic bag mouth is sealed with sealing machine immediately after charging and inoculation, is solved the ventilation in growth in this way and is needed to prevent again The problem of disease pest is invaded from sack in mycelia growth is stopped.The fungi-proofing cloth patch of growth in polybag is also to have wanting for profession Ask, it not only needs to ventilate, be satisfied with growth needs in bag, can not venthole it is excessive or excessive and with seed in bag walking Moisture influences mycelia growth, while being also required to have aeration again and can stop one kind of multiple-microorganism is dedicated fungi-proofing to breathe freely Cloth --- i.e. fungi-proofing cloth patch is as the Special ventilating film in strain bag.In order to by the germ often occurred in growth and pest It is filtered by fungi-proofing cloth patch, we search and than right Trichoderma viride, Pseudomonas thallus, bacillus subtilis bud The Individual Size data of spore, yeast, tiny anhui fritillary and rhabditis axei ovum, and will be needed for these biologies and mycelia growth The volume size of oxygen, carbon dioxide and hydrone is compared, and the results are shown in Table 2, it can be seen from the table oxygen molecule, titanium dioxide Carbon, hydrone volume be nanoscale, be respectively smaller than 1800 times of Pseudomonas, 3000 times of yeast, 4000 times of Trichoderma viride, more Nematode and ten thousand times of acarid or more are less than, to select the fungi-proofing cloth patch in suitable aperture to provide effective foundation.In this data basis On selected from the fungi-proofing cloth in the 0.01-aperture 0.05um patch strain bag respectively with 6 0.5 differential carry out production of hybrid seeds bacterium germination examinations It tests, observes temperature tolerance, aeration, flatulence, mushroom mycelium upgrowth situation, bacterial growth situation and the fungus growth situation of bacterium bag Deng 6 correlation factors, show that 4 data of table, test result are shown with the 0.01-hole 0.05um by multiple batches of screening test The indices of the mushroom of the fungi-proofing cloth patch strain bag culture of diameter are normal, and aeration is preferable in bacterium bag, bacterium bag does not occur Interior flatulence phenomenon；Water-in-bag part is sufficient, and fungi-proofing cloth patch window culture medium moisture content is kept, and mycelia growth is normal；Especially bacterium bag Miscellaneous bacteria amount significantly reduces in incubation, and kind bag yield rate is increased to 99.6%, stable product quality.Pass through more batches more than 2 years Determine that the fungi-proofing cloth patch using the 0.01-aperture 0.05um grows Spawn incubation bag as edible fungus species after secondary test.

2 predominant gas of table, microorganism and pest volume size compare

3 Pollution In Edible Mushroomss kind mycelium width of table

The fungi-proofing cloth of table 4 patch aperture performance compares

The fungi-proofing cloth patch is overlapped and integral by non-woven fabrics with microporous teflon membran, determines aperture using ultrasonic wave 2 materials are suppressed and integral in a mold afterwards, non-woven fabrics cuts body as microporous teflon membran, makes it and high pressure Polyethylene film pastes the ventilation window that strain bag is made.

3, fungi-proofing cloth patch strain bag production

This patent proposes that Spawn incubation bag is bonded by Spawn incubation bag body and fungi-proofing cloth patch；Spawn incubation bag body upper end There is sack, obtained fungi-proofing cloth patch is pasted onto below sack from the position 160~200mm of sealing part, while wiping out fungi-proofing cloth patch The bag body parts of plastics of inside is pasted using fungi-proofing cloth as the ventilative window of strain bag.Spawn incubation process is put with stand-type, Therefore fungi-proofing cloth patch position exists without matrix, is conducive to air exchange.

4, the application of Spawn incubation bag

Strain productive culture based formulas applied by Spawn incubation bag is 2 kinds: 1, millet 85%, fermented maize core powder 7%, Neutral calcium carbonate 5%, edible plaster 2%, lime 1%.This formula be applied to production straw rotting fungus kind, e.g., dictyophora phalloidea, coprinus comatus, Stropharia rugoso-annulata, agaricus bisporus and Brazilian mushroom；2, weedtree sawdust 52%, cotton seed hulls 15%, maize cob meal 15%, wheat bran 15%, in Property calcium carbonate 1%, edible plaster 1%, lime 1%.This formula is for producing domestomycetes kind, such as Phellinus, Pleurotus ferulae, grifola frondosus With mushroom etc..

Strain production process: carrying out clear water immersion for dry millet by recipe requirements before this, fishes out when water absorption is up to 40% It rises, carries out steam boiling, kept for 50 minutes up to 100 DEG C, release millet after steamed and shakeout while hot on ground；It will be required in formula again Calcium carbonate, gypsum and lime and millet stir evenly, be eventually adding maize cob meal and stirred evenly with millet, the culture being stirred Moisture can pack into sterilizing cabinet up to 43-45% and carry out autoclave sterilization base after measured；Sterilising temp controls 120 DEG C of guarantors 4 hours are held, millet is spread out into sterilizing cabinet when temperature drops to 60 DEG C, strain bag is allowed aseptically to continue cool to It is inoculated at 25 DEG C；Sack is opened in 100 grades of air cleaning transfer rooms and accesses strain, closes sack immediately, and overturn bag Son is uniformly mixed strain in bag with culture medium, and strain bag is placed on bacteria-producing room bacterium germination.Bacterium germination temperature in bag during inoculation 15 days Strain is moved into 14-16 DEG C of bacterium germination libraries again after bacterium bag mycelia is up-to-standard on inspection and continues culture 15 by 18-20 DEG C of degree control It sends out full full bag to hyphae length, and bacterium bag is white but water-in-bag point increases, and need to again move in 5 DEG C of libraries bacterium bag and carry out receipts water planting It supports 5-10 days, it is whole strain mycelial growth time 40-50 days, anhydrous to ooze now when culture medium presentation white in sack, open bag Survey is covered with mycelia on grain, and mycelia is not agglomerated in bag, and hand is grabbed fungus block and easily dispersed, and mushroom fragrance is dense, indicates strain bacterium Silk culture terminates, can be by strain preservation waiting vanning sale at low temperature.

Carrying out this specification strain bag 2 years in edible mushroom dictyophora phalloidea, coprinus comatus, Stropharia rugoso-annulata, agaricus bisporus, Brazilian mushroom, perfume (or spice) more The mycelia such as mushroom, Pleurotus ferulae, grifola frondosus and Phellinus, which grow in more slow edible fungus variety and its strain production, is applied. Our company does the strain of the kind of a variety of mycelia slow growths of container production using fungi-proofing cloth patch strain bag, shows from table 5, Its mycelia fast growing, incubation time shorten obviously, and strain yield rate significantly improves.The mycelia purseful time 30 days or so, bacterium Contamination rate control is below 0.6%；Mould contamination rate 0.3%；Yeast pollution rate 0%；Acarid growth rate 0%；Kind bag yield rate Up to 99% or more.

The application of the fungi-proofing cloth patch strain bag in the aperture 5 0.01~0.05um of table.

Claims (5)

1. The Spawn incubation bag of fungi-proofing function 1. a kind of tool is ventilated, it is characterised in that: the Spawn incubation bag is by Spawn incubation bag body (1) it is constituted with fungi-proofing cloth patch (2)；Have in Spawn incubation bag body (1) sack (3), fungi-proofing cloth patch (2) be sticked to below sack (3) from The position 160~200mm of sealing part, while wiping out the pocket portion of fungi-proofing cloth patch inside.
2. 2. Spawn incubation bag according to claim 1, it is characterised in that: fungi-proofing cloth patch aperture is 0.01~ 0.05um, fungi-proofing cloth paste long 110mm × wide 60mm, and number cells are up to 5000-6000.
3. 3. Spawn incubation bag according to claim 1 or 2, it is characterised in that: the fungi-proofing cloth patch is by non-woven fabrics and poly- four Fluoride microporous film coincidence is suppressed and integral in a mold, determines aperture using ultrasonic wave, and fungi-proofing cloth patch aperture is 0.01~ 0.05um；The microporous teflon membran is made of polyflon, and membrane aperture is required in 0.01-0.05um, thick 50-80um are spent, for porosity up to 80%, the nonwoven thickness is 50g/m²。
4. 4. Spawn incubation bag according to claim 1 or 2, it is characterised in that: the Spawn incubation bag body (1) is a kind of tool Transparent high temperature resistant high-pressure polypropylene CPP film five-layer co-squeezing synthesizes the polybag of film production, according to production parent species, strain and cultivation The formula and matrix weight of kind, respectively three kinds of bacterium bag specifications, parent species bacterium bag specification are as follows: long by 300 × wide by 180 × thick 0.07mm；Original seed bacterium bag specification: long 350 × wide by 220 × thickness 0.07mm；Cultivar bacterium bag specification are as follows: long by 450 × wide by 300 × thick 0.07mm。
5. 5. Spawn incubation bag according to claim 3, it is characterised in that: the Spawn incubation bag body (1) is that a kind of tool is saturating The polybag of bright high temperature high voltage resistant polypropylene CPP film five-layer co-squeezing synthesis film production, according to production parent species, strain and cultivar Formula and matrix weight, respectively three kinds of bacterium bag specifications, parent species bacterium bag specification are as follows: long 300 × wide by 180 × thickness 0.07mm；It is former Kind bacterium bag specification: long 350 × wide by 220 × thickness 0.07mm；Cultivar bacterium bag specification are as follows: long 450 × wide by 300 × thickness 0.07mm.