CN207944108U - A kind of Tissue Culture Dish applying three-dimensional magnetic field - Google Patents
A kind of Tissue Culture Dish applying three-dimensional magnetic field Download PDFInfo
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- CN207944108U CN207944108U CN201820177382.9U CN201820177382U CN207944108U CN 207944108 U CN207944108 U CN 207944108U CN 201820177382 U CN201820177382 U CN 201820177382U CN 207944108 U CN207944108 U CN 207944108U
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- magnetic field
- culture dish
- wafer
- supporting platform
- tissue culture
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Abstract
A kind of Tissue Culture Dish applying three-dimensional magnetic field, including culture dish, culture ware lid is equipped at the top of culture dish, culture dish is fixed at vertical magnetic field and on piece occurs, and the magnetic field that piece is used to generate vertical direction occurs for vertical magnetic field, vertical magnetic field occurs to be equipped with wafer-supporting platform below piece, vertical magnetic field occurs to be flexibly connected between piece and wafer-supporting platform, and wafer-supporting platform is set on the base, and the pedestal of wafer-supporting platform both sides is equipped with magnet slot, electromagnet is equipped in magnet slot, electromagnet is used to generate the magnetic field of horizontal direction.Using the above structure, the magnetic field that multi-angle can be applied to culture dish, to preferably influence of the research magnetic field to cell.
Description
Technical field
The utility model is related to cell culture apparatus field, especially a kind of Tissue Culture Dish applying three-dimensional magnetic field.
Background technology
Cell culture refers to normal physiological environment and nutritional condition in analogue body under in vitro conditions, is separately cultured body
Cell or the method for establishing cell line, and making cell be grown for a long time in culture vessel, being proliferated.Cell culture is in artificial condition
Lower progress, have the advantages that be easy to regulate and control and be convenient for observation, in molecular genetics, molecular biology, modern medicine, bioid
Etc. fields have important application value.For example, the fetal cell in amniotic fluid that falls off is obtained by amniocentesis, through meticulous
Chromosome analysis is carried out after born of the same parents' culture, whether diagnosable fetus suffers from genetic disease, technical guarantee is provided for prenatal and postnatal care.Closely
Year is the study found that outfield stimulates（Such as electric field, gravitational field, light field, magnetic field）It may cause the stress reaction for cultivating cell, to
Its proliferation, differentiation and form, structure, function are influenced, or even influences the construction of its internal organelle.Outfield is furtherd investigate to cell
Influence, had important practical significance in researchs in metabolism, chromosomal variation, inhereditary material expression with regulating and controlling etc..It grinds
Study carefully influence of the outfield to cell, new challenge is not only proposed to cell culture technology, while also being proposed to cell culture apparatus
New requirement.
Magnetic field, as a kind of specific form of substance objective reality, disperse is in wide physical space.Studies have shown that
There may be significant cell biocompatibilities for specific magnetic fields, such as：The reddish brown worm of wheat is after low-intensity magnetic field irradiates a couple of days, cell division
Speed can be dramatically speeded up；Steady magnetic field can reduce the content of cAMP in the Pineal Body In Raties cyclic adenosine monophosphate decorum；Low frequency magnetic field
Or static steady magnetic field is to leukemia cell line（Jurkat Clone E6-1）Proliferation have a significant impact.Although at present
Through having carried out some similar researchs, but due to differing greatly between cell category, and the adjustability of magnetic field parameter is strong so that people
There is huge exploration space on the cell biocompatibility in understanding magnetic field.At present in this Quasi-experiment study, cell culture with
Magnetic field occurs often to use discrete device, is not only unfavorable for experimental implementation, but also be not easy to experiment contrast.
In conclusion providing a kind of Tissue Culture Dish device applying three-dimensional magnetic field, will be given birth to for the cell for studying magnetic field
It is greatly convenient that object effect provides, and has significant application value.The country not yet finds similar device at present.
Invention content
The technical problem to be solved by the utility model is to provide a kind of Tissue Culture Dish applying three-dimensional magnetic field, can
The magnetic field for applying multi-angle to culture dish, to preferably influence of the research magnetic field to cell.
In order to solve the above technical problems, technical solution used by the utility model is：A kind of three-dimensional magnetic field of applying
Tissue Culture Dish, including culture dish, culture dish top are equipped with culture ware lid, and culture dish is fixed at vertical magnetic field and piece occurs
On, the magnetic field that piece is used to generate vertical direction occurs for vertical magnetic field, and vertical magnetic field occurs to be equipped with wafer-supporting platform, vertical magnetic field below piece
Occur to be flexibly connected between piece and wafer-supporting platform, wafer-supporting platform is set on the base, and the pedestal of wafer-supporting platform both sides is equipped with magnet slot, magnetic
Electromagnet is equipped in metal trough, electromagnet is used to generate the magnetic field of horizontal direction.
In preferred scheme, the vertical magnetic field occurs piece bottom surface and is equipped with anchorage bar, and the center of wafer-supporting platform is equipped with
Anchorage bar mounting hole, anchorage bar, which is inserted into, to be arranged in anchorage bar mounting hole.
In preferred scheme, the anchorage bar is equipped with thread segment, and the thread segment of anchorage bar is equipped with nut, and nut is set
It sets on the anchorage bar of wafer-supporting platform subjacent, the nut outer wall is equipped with Antiskid handle.
In preferred scheme, the vertical magnetic field occurs piece side wall and is equipped with mark line, on the wafer-supporting platform side wall
Equipped with angular range graticule.
In preferred scheme, the pedestal is divided into pedestal upper layer and pedestal lower layer, and pedestal upper layer and pedestal lower layer are two
The different cylindrical structure of a diameter of section, and the diameter of section on pedestal upper layer is less than the diameter of section of pedestal lower layer, described holds
Piece platform is fixed on pedestal upper layer；
The pedestal lower layer top surface is equipped with annular fence and outer ring fence, annular fence and outer ring fence
It is fixed at by fence supporting rod on the top surface of pedestal lower layer, annular fence and outer ring fence are equipped with vertical branch
Fagging, support plate bottom is equipped with passes through arc with annular fence and the matched arc bayonet of outer ring fence, support plate bottom
Bayonet is fixed on annular fence and outer ring fence；
The magnet slot is arranged at the top of support plate.
In preferred scheme, the magnet slot is "u"-shaped groove structure, and electromagnet is fixed in magnet slot, magnet
The inner bottom surface of slot is equipped with groove, and electromagnet conducting wire is equipped in groove, and the magnet slot lateral wall lower edge is equipped with graduation mark.
In preferred scheme, the magnet groove sidewall is equipped with bolt slot, and it is tight to be equipped with horizontal direction in bolt slot
Fixing bolt, the fastening end of fastening bolt is stretched into across bolt slot in magnet slot to be contacted with electromagnet, realizes electromagnet in magnet
Positioning in slot.
In preferred scheme, the wafer-supporting platform top surface is equipped with the ball grooves of annular, and ball, ball are equipped in ball grooves
Piece bottom surface occurs with vertical magnetic field to contact.
In preferred scheme, the culture dish outer wall is equipped at least two equally distributed bayonet inserted sheets, described
The pedestal upper layer top surface of wafer-supporting platform both sides is equipped with adherent column, is equipped with and the matched bayonet of bayonet inserted sheet at the top of adherent column.
In preferred scheme, the wafer-supporting platform is equipped at least one first wire guide, and the pedestal upper layer is equipped with
At least one second wire guide, the adherent column are equipped with oblique threading hole, and the pedestal lower layer side wall is equipped with third
Wire guide.
In preferred scheme, the vertical magnetic field occurs 5 top surface of piece and is equipped with multiple metal strips 37 disposed in parallel, gold
It is electrode 36 to belong to 37 both ends of item, and electrode 36 is connect by conducting wire 38 with power supply；
In preferred scheme, the vertical magnetic field occurs that multiple square crossing setting metal strips 37 are arranged on 5 top surface of piece,
37 both ends of metal strip are electrode 36, and electrode 36 is connect by conducting wire 38 with power supply；
In preferred scheme, the vertical magnetic field occurs 5 top surface of piece and is equipped with multiple magnetic patch 39.
A kind of Tissue Culture Dish applying three-dimensional magnetic field provided by the utility model, by using above structure, tool
There is following advantageous effect：
（1）The purpose for applying three-dimensional magnetic field to the cell in culture dish is realized, to easily facilitate research magnetic field to thin
The influence of born of the same parents；
（2）The power in magnetic field and direction can voluntarily be adjusted, so as to study the magnetic field force pair of different directions, size
The influence of cell in culture dish.
Description of the drawings
The utility model is described in further detail with reference to the accompanying drawings and examples：
Fig. 1 is the overall structure diagram of the utility model.
Fig. 2 is the roll structure schematic diagram at the top of the utility model vertical magnetic field generation piece.
Fig. 3 is the magnet slot structure schematic diagram of the utility model.
Fig. 4-6 is that piece top schematic diagram occurs for the different vertical magnetic field of the utility model.
In figure：Cultivate ware lid 1, culture dish 2, bayonet inserted sheet 3, the vertical magnetic field magnetic line of force 4, vertical magnetic field generation piece 5, label
Line 6, anchorage bar 7, thread segment 8, nut 9, Antiskid handle 10, the first wire guide 11, anchorage bar mounting hole 12, ball 13 hold piece
Platform 14, bayonet 15, oblique threading hole 16, adherent column 17, angular range graticule 18, the horizontal magnetic field magnetic line of force 19, electromagnet 20, magnet
Slot 21, graduation mark 22, support plate 23, the second wire guide 24, annular fence 25, outer ring fence 26, pedestal upper layer 27, fence
Supporting rod 28, privates hole 29, pedestal lower layer 30, fastening bolt 31, groove 32, electromagnet conducting wire 33, bolt slot 34, arc
Shape bayonet 35, electrode 36, metal strip 37, conducting wire 38, magnetic patch 39, ball grooves 40.
Specific implementation mode
Embodiment 1：
In Fig. 1, a kind of Tissue Culture Dish applying three-dimensional magnetic field, including culture dish 2,2 top of culture dish are equipped with training
Ware lid 1 is supported, culture dish 2 is fixed at vertical magnetic field and occurs on piece 5, and piece 5 occurs for vertical magnetic field for generating vertical direction
Magnetic field, in magnetic field line such as Fig. 1 shown in the vertical magnetic field magnetic line of force 4, vertical magnetic field occurs 5 lower section of piece and is equipped with wafer-supporting platform 14, perpendicular magnetic
Field occurs to be flexibly connected between piece 5 and wafer-supporting platform 14, and wafer-supporting platform 14 is set on the base, and the pedestal of 14 both sides of wafer-supporting platform is equipped with
Magnet slot 21, is equipped with electromagnet 20 in magnet slot 21, and electromagnet 20 is used to generate the magnetic field of horizontal direction, in magnetic field line such as Fig. 1
Shown in the horizontal magnetic field magnetic line of force 19.
In preferred scheme, the vertical magnetic field occurs 5 bottom surface of piece and is equipped with anchorage bar 7, on the center of wafer-supporting platform 14
Equipped with anchorage bar mounting hole 12, anchorage bar 7, which is inserted into, to be arranged in anchorage bar mounting hole 12.
In preferred scheme, the anchorage bar 7 is equipped with thread segment 8, and the thread segment 8 of anchorage bar 7 is equipped with nut 9,
Nut 9 is arranged on the anchorage bar 7 of 14 subjacent of wafer-supporting platform, and 9 outer wall of nut is equipped with Antiskid handle 10.
In preferred scheme, the vertical magnetic field occurs 5 side wall of piece and is equipped with mark line 6,14 side of wafer-supporting platform
Wall is equipped with angular range graticule 18.
In preferred scheme, 2 outer wall of culture dish is equipped at least two equally distributed bayonet inserted sheets 3, described
27 top surfaces of pedestal upper layer of 14 both sides of wafer-supporting platform be equipped with adherent column 17, the top of adherent column 17 is equipped with and bayonet inserted sheet 3
Matched bayonet 15.
Embodiment 2：
On the basis of embodiment 1, the pedestal is divided into pedestal upper layer 27 and pedestal lower layer 30, pedestal upper layer 27 and bottom
Seat lower layer 30 is the different cylindrical structure of two diameter of sections, and the diameter of section on pedestal upper layer 27 is less than cutting for pedestal lower layer 30
Face diameter, the wafer-supporting platform 14 are fixed on pedestal upper layer 27；
30 top surface of pedestal lower layer is equipped with annular fence 25 and outer ring fence 26, annular fence 25 and outer
Circular fence 26 is fixed at by fence supporting rod 28 on the top surface of pedestal lower layer 30, and annular fence 25 and outer ring are enclosed
Column 26 is equipped with vertical support plate 23, and 23 bottom of support plate is equipped with and 26 matched arc of annular fence 25 and outer ring fence
Shape bayonet 35,23 bottom of support plate are fixed on by arc bayonet 35 on annular fence 25 and outer ring fence 26；
The setting of magnet slot 21 is at 23 top of support plate.
On the basis of using the above structure, by adjusting position of the support plate 23 in circular fence 25,26, so as to
Enough adjust the direction of the horizontal magnetic field magnetic line of force 19 of two horizontal magnetic fields, the horizontal magnetic field magnetic force caused by two horizontal magnetic fields
When the angle of line 19 is not 180 °, whole device generates three-dimensional magnetic field, and angle can be between two magnetic fields of horizontal direction
It is arbitrary to adjust, convenient for the cellular change under the conditions of research different magnetic field.
Embodiment 3：
Such as Fig. 3, the magnet slot 21 is "u"-shaped groove structure, and electromagnet 20 is fixed in magnet slot 21, magnet slot
21 inner bottom surface is equipped with groove 32, and electromagnet conducting wire 33 is equipped in groove 32, is set in the 21 lateral wall lower edge of magnet slot
There is graduation mark 22.
In preferred scheme, 21 side wall of magnet slot is equipped with bolt slot 34, and level is equipped in bolt slot 34
To fastening bolt 31, the fastening end of fastening bolt 31 stretches into magnet slot 21 across bolt slot 34 and contacted with electromagnet 20,
Realize positioning of the electromagnet 20 in magnet slot 21.
Embodiment 4：
Such as Fig. 2, on the basis of embodiment 1,14 top surface of wafer-supporting platform is equipped with the ball grooves 40 of annular, ball grooves
Ball 13 is equipped in 40, ball 13 occurs 5 bottom surface of piece with vertical magnetic field and contacts.
Embodiment 5：
On the basis of embodiment 1,2,3, the wafer-supporting platform 14 is equipped at least one first wire guide 11, described
Pedestal upper layer 27 is equipped at least one second wire guide 24, and the adherent column 17 is equipped with oblique threading hole 16, the bottom
30 side wall of seat lower layer is equipped with privates hole 29.
The conducting wire that piece 5 occurs for vertical magnetic field is worn by the first wire guide 11, the second wire guide 24 and privates hole 29 successively
It crosses, the electromagnet conducting wire 33 of electromagnet 20 is passed through by oblique threading hole 16, the second wire guide 24 and privates hole 29 successively
The vertical magnetic field occurs 5 top surface of piece and is equipped with the structure for being used for figure magnetizing field；
The structure can be multiple metal strips disposed in parallel 37, and 37 both ends of metal strip are electrode 36, and electrode 36 is by leading
Line 38 is connect with power supply（Such as Fig. 4）；
Can be multiple square crossing setting metal strips 37,37 both ends of metal strip are electrode 36, and electrode 36 passes through conducting wire 38
It is connect with power supply（Such as Fig. 5）；
Can also be multiple magnetic patch 39（Such as Fig. 6）.
After vertical magnetic field occurs to be provided with the above-mentioned structure for figure magnetizing field on 5 top surface of piece, sight can be passed through
Magnetic field after examining graphically carries out the adjusting in vertical direction magnetic field.
Conducting wire 38 when using first two structure connects external power supply, supply voltage 0-220V, direct current supply.
When using magnetic patch 39, magnetic field is generated using the Fe/Ni permanent-magnet alloy square arrays of sputtering.
Embodiment 6：
On the basis of embodiment 1-5：
Wafer-supporting platform 14, adherent column 17 are acrylonitrile-butadiene-phenylethylene copolymer plastics material；
Magnet slot 21 uses vinyon material, entire horizontal magnetic field generating mechanism to use Demountable.
Electromagnet 20 can be changed to magnet, and it is 2 × 2 × 4 centimetres that magnet, which uses cuboid rare-earth permanent magnet, size,.
The course of work of whole device is as follows：
Device is placed in cell culture operations case.The anchorage bar 7 that vertical magnetic field to that 5 lower end of piece occur passes through wafer-supporting platform
The anchorage bar mounting hole 12 in 14 centers, rotation vertical magnetic field are occurred piece 5 to design angle, vertical magnetic field are occurred using nut 9
Piece 5 is fastened on wafer-supporting platform 14, vertical magnetic field to that conducting wire the first wire guide 11, the second wire guide 24 of piece 6 occur, finally the bottom of from
The privates hole 29 of 30 side wall of seat lower layer is pierced by, and is connected with external power supply, is adjusted power supply size, alternating current-direct current form, to obtain
Design magnetic field；
By horizontal magnetic field generation part（That is 21 part of magnet slot）The symmetrical circular fence 25,26 for being fixed on pedestal
On, depending on 21 number of magnet slot is according to specific experiment design, 21 position of regulating magnet slot obtains the magnetic field of different directions；If magnetic
Iron use electromagnet when, the adjusting of magnetic field size may be implemented, by conducting wire from oblique threading hole 16, the second wire guide 24, finally from
The privates hole 29 of 30 side wall of pedestal lower layer is pierced by, and is connect with external power supply；
Hereafter, glass culture dish 2 is inserted by bayonet inserted sheet 3 on the bayonet of 17 upper end of adherent column；
Cell culture medium and culture cell are added in culture dish 2, covers culture ware lid 1, observation cell is under magnetic field radiation
Growth conditions.
Claims (10)
1. a kind of Tissue Culture Dish applying three-dimensional magnetic field, including culture dish（2）, it is characterized in that：Culture dish（2）Top is equipped with
Cultivate ware lid（1）, culture dish（2）It is fixed at vertical magnetic field and piece occurs（5）On, piece occurs for vertical magnetic field（5）For generating
Piece occurs for the magnetic field of vertical direction, vertical magnetic field（5）Lower section is equipped with wafer-supporting platform（14）, vertical magnetic field generation piece（5）With wafer-supporting platform
（14）Between be flexibly connected, wafer-supporting platform（14）It is set on the base, wafer-supporting platform（14）The pedestal of both sides is equipped with magnet slot（21）,
Magnet slot（21）It is interior to be equipped with electromagnet（20）, electromagnet（20）Magnetic field for generating horizontal direction.
2. a kind of Tissue Culture Dish applying three-dimensional magnetic field according to claim 1, it is characterised in that：Described is vertical
Piece occurs for magnetic field（5）Bottom surface is equipped with anchorage bar（7）, wafer-supporting platform（14）Center be equipped with anchorage bar mounting hole（12）, fastening
Bar（7）It is inserted into and is arranged in anchorage bar mounting hole（12）In.
3. a kind of Tissue Culture Dish applying three-dimensional magnetic field according to claim 2, it is characterised in that：The fastening
Bar（7）It is equipped with thread segment（8）, anchorage bar（7）Thread segment（8）It is equipped with nut（9）, nut（9）It is arranged in wafer-supporting platform
（14）The anchorage bar of subjacent（7）On, the nut（9）Outer wall is equipped with Antiskid handle（10）.
4. a kind of Tissue Culture Dish applying three-dimensional magnetic field according to claim 2, it is characterised in that：Described is vertical
Piece occurs for magnetic field（5）Side wall is equipped with mark line（6）, the wafer-supporting platform（14）Side wall is equipped with angular range graticule（18）.
5. a kind of Tissue Culture Dish applying three-dimensional magnetic field according to claim 1, it is characterised in that：The pedestal
It is divided into pedestal upper layer（27）With pedestal lower layer（30）, pedestal upper layer（27）With pedestal lower layer（30）It is different for two diameter of sections
Cylindrical structure, and pedestal upper layer（27）Diameter of section be less than pedestal lower layer（30）Diameter of section, the wafer-supporting platform（14）
It is fixed at pedestal upper layer（27）On；
The pedestal lower layer（30）Top surface is equipped with annular fence（25）With outer ring fence（26）, annular fence（25）
With outer ring fence（26）Pass through fence supporting rod（28）It is fixed at pedestal lower layer（30）Top surface on, annular fence
（25）With outer ring fence（26）It is equipped with vertical support plate（23）, support plate（23）Bottom is equipped with and annular fence（25）
With outer ring fence（26）Matched arc bayonet（35）, support plate（23）Bottom passes through arc bayonet（35）In being fixed on
Circular fence（25）With outer ring fence（26）On；
The magnet slot（21）It is arranged in support plate（23）Top.
6. a kind of Tissue Culture Dish applying three-dimensional magnetic field according to claim 5, it is characterised in that：The magnet
Slot（21）For "u"-shaped groove structure, electromagnet（20）It is fixed at magnet slot（21）It is interior, magnet slot（21）Inner bottom surface be equipped with
Groove（32）, groove（32）It is interior to be equipped with electromagnet conducting wire（33）, the magnet slot（21）Lateral wall lower edge is equipped with graduation mark
（22）.
7. a kind of Tissue Culture Dish applying three-dimensional magnetic field according to claim 6, it is characterised in that：The magnet
Slot（21）Side wall is equipped with bolt slot（34）, bolt slot（34）It is interior to be equipped with horizontal direction fastening bolt（31）, fastening bolt
（31）Fastening end pass through bolt slot（34）Stretch into magnet slot（21）Interior and electromagnet（20）Electromagnet is realized in contact（20）
Magnet slot（21）Interior positioning.
8. a kind of Tissue Culture Dish applying three-dimensional magnetic field according to claim 6, it is characterised in that：Described holds piece
Platform（14）Top surface is equipped with the ball grooves of annular（40）, ball grooves（40）It is interior to be equipped with ball（13）, ball（13）With vertical magnetic field
Piece occurs（5）Bottom surface contacts.
9. a kind of Tissue Culture Dish applying three-dimensional magnetic field according to claim 5, it is characterised in that：The culture
Ware（2）Outer wall is equipped at least two equally distributed bayonet inserted sheets（3）, the wafer-supporting platform（14）The pedestal upper layer of both sides
（27）Top surface is equipped with adherent column（17）, adherent column（17）Top is equipped with and bayonet inserted sheet（3）Matched bayonet（15）.
10. a kind of Tissue Culture Dish applying three-dimensional magnetic field according to claim 9, it is characterised in that：Described holds
Piece platform（14）It is equipped at least one first wire guide（11）, the pedestal upper layer（27）Equipped at least one second wire guide
（24）, the adherent column（17）It is equipped with oblique threading hole（16）, the pedestal lower layer（30）Side wall is led equipped with third
String holes（29）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820177382.9U CN207944108U (en) | 2018-02-01 | 2018-02-01 | A kind of Tissue Culture Dish applying three-dimensional magnetic field |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820177382.9U CN207944108U (en) | 2018-02-01 | 2018-02-01 | A kind of Tissue Culture Dish applying three-dimensional magnetic field |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207944108U true CN207944108U (en) | 2018-10-09 |
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ID=63698724
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820177382.9U Expired - Fee Related CN207944108U (en) | 2018-02-01 | 2018-02-01 | A kind of Tissue Culture Dish applying three-dimensional magnetic field |
Country Status (1)
| Country | Link |
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| CN (1) | CN207944108U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108130275A (en) * | 2018-02-01 | 2018-06-08 | 三峡大学 | A kind of Tissue Culture Dish for applying three-dimensional magnetic field |
| CN110283723A (en) * | 2019-06-26 | 2019-09-27 | 西北工业大学深圳研究院 | A kind of culture apparatus |
-
2018
- 2018-02-01 CN CN201820177382.9U patent/CN207944108U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108130275A (en) * | 2018-02-01 | 2018-06-08 | 三峡大学 | A kind of Tissue Culture Dish for applying three-dimensional magnetic field |
| CN110283723A (en) * | 2019-06-26 | 2019-09-27 | 西北工业大学深圳研究院 | A kind of culture apparatus |
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Granted publication date: 20181009 Termination date: 20220201 |