CN104745471A - Electricity-force stimulating cell culture apparatus - Google Patents
Electricity-force stimulating cell culture apparatus Download PDFInfo
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- CN104745471A CN104745471A CN201510128360.4A CN201510128360A CN104745471A CN 104745471 A CN104745471 A CN 104745471A CN 201510128360 A CN201510128360 A CN 201510128360A CN 104745471 A CN104745471 A CN 104745471A
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- electricity
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- pole plate
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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Abstract
The invention provides an electricity-force stimulating cell culture apparatus, which comprises a cell cultivator and a signal generator, wherein the cell cultivator comprises a cultivator base; a conductive film is arranged on the cultivator base; an electricity-force stimulating electrode plate is arranged above the cell cultivator, and comprises an electricity-force stimulating electrode base on the upper part, a conductive layer on the middle part and a piezoelectric stimulator on the lower part; and a positive electrode and a negative electrode of the signal generator are respectively connected with the conductive layer on the electricity-force stimulating electrode plate and the conductive film on the cell cultivator. When cell cultivation is carried out, electric pulse stimulation can be provided for cells and a culture medium in the cell cultivator; after the piezoelectric stimulator of the electricity-force stimulating electrode plate is electrified, micro-vibration can be generated under the action of current, and the force stimulation equivalent to peripheral cell peristalsis in a living body is provided for the cells. The electricity-force stimulating cell culture apparatus is capable of really simulating the cell stress environment in the living body; and an environment which is relatively close to cell growth environment in the living body is provided for in vitro culture of the cells.
Description
Technical field
The invention belongs to biomedicine, cell engineering and tissue engineering technique field, particularly relate to a kind of can cell growth process carry out electricity and power stimulation electricity-Li irritation cell culture apparatus.
Background technology
In modern biomedical, along with the widespread use in clinical medicine of body implant and histoorgan, Cell culture invitro is made to become biomedical gradually and important research means in Tissue Engineering Study.Vitro culture conditions and organism inner cell grow the degree of agreement of actual environment, determine the verity of cell research result.
Cell in organism carries out signal communication mainly through bioelectrical signals and chemical signal, the primary condition that electricity irritation is cell conductance information, produces division, breaks up and move.The pulsed voltage that electric field not only can provide cell to need, and the pulse signal that electric field produces also may play certain promotion or restraining effect to the growthhabit of cell and intercellular substance and behavior.In the presence of electric field, the division of cell, differentiation and transition process may be distinct with the process of growth of cell under free state.Electrical field stimulation is used for the real growing environment can simulating organism inner cell in Cell culture invitro process really, thus makes cell cultures result more accurately credible.In addition, cell is when life tumor growth, cell peripheral tissue, body fluid or blood directly can produce the effect of power to cell, as muscle cell bear pulling force or pressure when human body moves, cranial nerve cell bears pressure etc. under naturally the wriggling of cerebral tissue, its growing environment is different from static cultivation environment under external state of nature.Relevant research shows, the power acting on cell stimulates, and may affect form, structure and function during Growth of Cells.
China ZL201010243578.1 discloses a kind of cell culture apparatus of shearing force-electricity combined stimulation, it adopts signal generator to provide electricity irritation to cell culture apparatus, flat flow chamber is adopted to provide shearing force to stimulate, flat flow chamber is provided with stream socket, fluid is provided by upstream and downstream liquid storage bottle and peristaltic pump, this apparatus structure is complicated, fluid flowing is adopted to provide shearing force to stimulate, the size of wayward stimulation power and uniformity coefficient, especially stimulate the uniformity coefficient of power, in flat flow chamber, the stimulation power at each position can not ensure unanimously.
Summary of the invention
The object of this invention is to provide a kind of electricity-Li irritation cell culture apparatus closer to organism inner cell growing environment.
For achieving the above object, the technical solution used in the present invention is:
A kind of electricity-Li irritation cell culture apparatus, comprise cell culture apparatus and signal generator, described cell culture apparatus comprises incubator substrate, and incubator substrate is provided with conducting film; Above described cell culture apparatus, be provided with electricity-Li stimulate pole plate, the electricity-Li that electricity-Li stimulates pole plate to comprise top stimulates pole plate substrate, the conductive layer at middle part and the piezoelectricity stimulator of bottom; Positive pole and the negative pole of described signal generator are connected electricity-Li respectively stimulates the conductive layer on pole plate and the conducting film on cell culture apparatus.
Described piezoelectricity stimulator is made up of piezoelectric, and its surface is provided with micro-nano structure, and shape is round boss, rectangular boss or bar shaped boss.
Described electricity-Li stimulates the base material of pole plate to be the silicon of applicable MEMS technology or micro-nano technology technique, quartzite or glass; Platinum, titanium or gold that described electricity-Li stimulates the conductive layer of pole plate that the thin film preparation process in MEMS technology or micro-nano technology technique can be adopted to prepare.
The shape of described cell culture apparatus is circle, rectangle or square vessel structure; Described cell culture apparatus base material is the insulating material that biocompatibility is good; The conducting film of described cell culture apparatus is the platinum of good conductivity and good biocompatibility, titanium, gold, iridium oxide or stainless steel.
The present invention compared with prior art tool has the following advantages:
1. piezoelectricity stimulator of the present invention adopts piezoelectric to make, its surface is provided with micro-nano structure, due to the material that piezoelectric is good biocompatibility, having converting electric energy is the ability of mechanical energy, therefore after electricity-Li stimulates pole plate energising, the microstructure adopting piezoelectric to make can produce micro-vibration, act in cell and substratum and can produce micro-reactive force to cell, be equivalent to surrounding tissue or cell in organism wriggle to the power stimulation of cell generation, the inner cell force environment of organism can be simulated truly;
2. the present invention carries out by the size of control signal producer received current the size regulating piezoelectricity stimulator micro-nano structure Oscillation Amplitude, thus change the size of power stimulation, micro-reactive force dynamics dynamics that the micro-vibration of piezoelectricity stimulator produces is even, be convenient to control, closer to cells in vivo force environment;
3. the present invention stimulates the conductive layer on pole plate to be connected with signal generator by wire with the conducting film on cell culture apparatus by electricity-Li, form current circuit, can be cell and real electric pulse stimulation in analogue body is provided, produce micro-vibration by piezoelectricity stimulator and provide real power environmental stimulation in analogue body for cell, make the environment that the vitro culture conditions of cell grows closer to organism inner cell;
4. structure of the present invention is simple, uses easy to operate.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Fig. 2 is that in the present invention, electricity-Li stimulates electrode plate structure partial enlarged drawing.
Embodiment
As illustrated in fig. 1 and 2, a kind of electricity-Li irritation cell culture apparatus, it stimulates pole plate 2 and cell culture apparatus 3 to form primarily of signal generator 1, electricity-Li.Electricity-Li stimulates pole plate 2 to stimulate pole plate substrate 4, the conductive layer 5 at middle part and the piezoelectricity stimulator 6 of bottom to form by the electricity-Li on top; Cell culture apparatus 3 is made up of incubator substrate 7 and conducting film 8; The positive pole of signal generator 1 is connected electricity-Li with negative pole respectively respectively by wire 10 stimulates the conductive layer 5 on pole plate 2 and the incubator conducting film 8 on cell culture apparatus 3.
Described electricity-Li stimulates piezoelectricity stimulator 6 surface of pole plate 2 to be provided with micro-nano structure, and shape can be round boss, rectangular boss or bar shaped boss, and MEMS technology or micro-nano technology technique can be adopted to make; Its material is the piezoelectric of good biocompatibility, and as polyvinylidene difluoride (PVDF) PVDF and multipolymer thereof, its thickness can be determined as required.
Substrate 4 material of described electricity-Li stimulation pole plate 2 is the base material of applicable MEMS technology or micro-nano technology technique, as silicon, quartzite or glass.
Described electricity-Li stimulates the conductive layer 5 of pole plate 2 that the thin film preparation process in MEMS technology or micro-nano technology technique can be adopted to prepare, and thickness is nanometer or micron order, for the material of good biocompatibility and good conductivity is made, as platinum, titanium or gold.
The shape of described cell culture apparatus 3 is vessel shape, can be circle, rectangle or square vessel structure, and the material of the incubator substrate 7 of cell culture apparatus 3 is that the insulating material that biocompatibility is good is made, as glass or quartzite; The conducting film 8 of cell culture apparatus 3 is made up of the material of good conductivity and good biocompatibility, and the thickness of conducting film is micron or nano level, as platinum, titanium, gold, iridium oxide or stainless steel.
When cell cultures, cell culture apparatus 3 is for holding cell and substratum 9, and piezoelectricity stimulator 6 inserts in cell and substratum 9; Signal generator 1 stimulates the conductive layer 5 on pole plate 2 and the conducting film 8 on cell culture apparatus 3 to provide pulsed current for electricity-Li, electric current stimulates the conducting film 8 on the cell in conductive layer 5, piezoelectricity stimulator 6 and cell culture apparatus 3 on pole plate 2 and substratum 9, cell culture apparatus 3 to form loop through electricity-Li, provide galvanism to cell and substratum 9; In addition, electricity-Li stimulates the conductive layer 5 on pole plate 2 to provide electric current for piezoelectricity stimulator 6, and the piezoelectric of piezoelectricity stimulator 6 produces micro-vibration under galvanic action, for the cell in cell culture apparatus 3 and substratum 9 provide the stimulation of power; The present invention adopts single signal producer stimulating electrical signal and power can be provided to stimulate for cell cultures, and structure is simple, uses easy to operate.
Claims (4)
1. an electricity-Li irritation cell culture apparatus, comprises cell culture apparatus and signal generator, it is characterized in that, described cell culture apparatus comprises incubator substrate, and incubator substrate is provided with conducting film; Above described cell culture apparatus, be provided with electricity-Li stimulate pole plate, the electricity-Li that electricity-Li stimulates pole plate to comprise top stimulates pole plate substrate, the conductive layer at middle part and the piezoelectricity stimulator of bottom; Positive pole and the negative pole of described signal generator are connected electricity-Li respectively stimulates the conductive layer on pole plate and the conducting film on cell culture apparatus.
2. electricity-Li irritation cell culture apparatus as claimed in claim 1, it is characterized in that, described piezoelectricity stimulator is made up of piezoelectric, and its surface is provided with micro-nano structure, and shape is round boss, rectangular boss or bar shaped boss.
3. electricity-Li irritation cell culture apparatus as claimed in claim 1 or 2, is characterized in that, the base material of described electricity-Li stimulation pole plate is the silicon of applicable MEMS technology or micro-nano technology technique, quartzite or glass; Platinum, titanium or gold that described electricity-Li stimulates the conductive layer of pole plate that the thin film preparation process in MEMS technology or micro-nano technology technique can be adopted to prepare.
4. electricity-Li irritation cell culture apparatus as claimed in claim 3, is characterized in that, the shape of described cell culture apparatus is circle, rectangle or square vessel structure; Described cell culture apparatus base material is the insulating material that biocompatibility is good; The conducting film of described cell culture apparatus is the platinum of good conductivity and good biocompatibility, titanium, gold, iridium oxide or stainless steel.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510128360.4A CN104745471B (en) | 2015-03-24 | 2015-03-24 | A kind of electric power stimulates cell culture apparatus |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510128360.4A CN104745471B (en) | 2015-03-24 | 2015-03-24 | A kind of electric power stimulates cell culture apparatus |
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| CN104745471A true CN104745471A (en) | 2015-07-01 |
| CN104745471B CN104745471B (en) | 2017-06-27 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108324293A (en) * | 2018-01-17 | 2018-07-27 | 北京航空航天大学 | A kind of skeletal muscle myotube dynamics measurement chip and preparation method |
| CN109957508A (en) * | 2017-12-25 | 2019-07-02 | 深圳先进技术研究院 | A kind of cytositimulation device and cell stimulation methodologies |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004329122A (en) * | 2003-05-08 | 2004-11-25 | Jgc Corp | Cell culture device and culture method |
| CN101250482A (en) * | 2008-03-28 | 2008-08-27 | 重庆大学 | Microelectrode array chip for cell electrofusion |
| CN102656260A (en) * | 2009-10-19 | 2012-09-05 | 瑞生生物技术有限公司 | Method, device and apparatus for inducing self-adjusting cell electroporation |
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2015
- 2015-03-24 CN CN201510128360.4A patent/CN104745471B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004329122A (en) * | 2003-05-08 | 2004-11-25 | Jgc Corp | Cell culture device and culture method |
| CN101250482A (en) * | 2008-03-28 | 2008-08-27 | 重庆大学 | Microelectrode array chip for cell electrofusion |
| CN102656260A (en) * | 2009-10-19 | 2012-09-05 | 瑞生生物技术有限公司 | Method, device and apparatus for inducing self-adjusting cell electroporation |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109957508A (en) * | 2017-12-25 | 2019-07-02 | 深圳先进技术研究院 | A kind of cytositimulation device and cell stimulation methodologies |
| CN108324293A (en) * | 2018-01-17 | 2018-07-27 | 北京航空航天大学 | A kind of skeletal muscle myotube dynamics measurement chip and preparation method |
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| CN104745471B (en) | 2017-06-27 |
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