CN203360449U - Multi-field coupling in vitro cell experiment device - Google Patents
Multi-field coupling in vitro cell experiment device Download PDFInfo
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- CN203360449U CN203360449U CN 201320426926 CN201320426926U CN203360449U CN 203360449 U CN203360449 U CN 203360449U CN 201320426926 CN201320426926 CN 201320426926 CN 201320426926 U CN201320426926 U CN 201320426926U CN 203360449 U CN203360449 U CN 203360449U
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Abstract
The utility model discloses a multi-field coupling in vitro cell experiment device, which comprises a container, a blind flange and a multi-functional sample stand, wherein an insulation interlayer and a magnetic stirrer are arranged in the container; a plurality of holes are formed in the surface of the blind flange; a filler and an outlet relief are respectively formed in the upper end and the lower end of the container; a ball valve is arranged at the outlet relief; the magnetic stirrer is used for balancing the bath temperature; the blind flange is connected to the top of the container to form a structure capable of opening or closing the container; the sample stand can be placed on an electromagnetic filed generation device with a porous cell culture plate and/or a single porous cell culture plate, and is placed in the container in experiment. The multi-field coupling in vitro cell experiment device has the advantages that the electromagnetic filed generation device with the porous cell culture plate is placed on the sample stand, and then is placed in the container, the atmosphere, temperature and humidity condition needed for cell culture is controlled, the structure is simple, the cost is low, the operation is convenient, the function is multiplex, and multiple groups of experiments can be carried out.
Description
Technical field
The utility model relates to a kind of cell culture apparatus, and especially a kind of many coupling In vitro cell experiment devices, belong to medical instruments field.
Background technology
Natural somatocyte is in complicated body microenvironment, and the effect of the stress-strain of natural body, bio-electric field, biological magnetic field affects the structure of body tissue growth, the development of morphology and function.From nineteen fifty-seven Fukada and Yasuda, the discovery of the macroforce electrical property of bone and the stress of Bassett discovery are subsequently promoted to the reason of bone reconstruction may be the current potential that stress produces in bone, stimulated cell response, induced the physiological change of cell.
Biomechanics has in clinical application: the limbs lengthening art of orthopaedics, the pressurization ligamentopexis of fracture, cranium Jaw distraction art and skin soft tissue stretching art etc.The action of a magnetic field aspect, there is animal experiment to find: under the action of a magnetic field, bone trabecula number and the thickness of experimental group all increase, the maximum tensile strength of femur and the maximum compressive strength of centrum all reach normally, and the bone surface such as human spine, femur and inside will produce time dependent induced current.By electro-magnetic biological effect, affect the osteocyte metabolism, improve bone density, improve bone mass, reach the purpose of prevention and treatment osteoporosis and fracture.Bioelectrical effect is the moulding of bone and rebuild important role, adopts electricity irritation to promote that union of fracture is the methods for the treatment of that orthopaedics is commonly used.Apply the formed charge accumulated of negative voltage at fracture and be conducive to union of fracture.A large amount of experimentation on animalies also prove, continuous direct current, and pulse direct current, continuous intravenous electric field and pulse electromagnetic field all can promote growth and the reparation of bone, and widespread use in clinical treatment.
Utilize the clinical treatment method of Biological Strength, electricity, magnetic field effect to be widely used, yet its Molecular Biology Mechanism is not set forth yet fully.In vitro study to active somatic cell under power, electricity, magnetic field environment to inquire in it the Molecular Biology Mechanism significant.The power that the research of In vitro cell experiment is set except structure, electricity, magnetic field, its experimental situation must adapt with conventional cell culture environment, otherwise can not accurately simulate the living organism internal milieu, causes its result of study to lack scientific basis.
At present, cell is applied to the experimental installation that the field of force, magnetic field and electric field studied four-point bending cell augmenter, pore pressurizing cell cultivation instrument, cell in vitro loading device with static magnetic field and cell electrophoresis apparatus etc., yet these equipment and instruments exist that application function is single, complex operation, price are high, can not independently carry out cell cultures, be difficult to realize that many group experiments carry out simultaneously, are unfavorable for studying the multiple not convenient factors such as experimental study of a large amount of samples of these type of needs of biomaterial and cell response.
The utility model content
The purpose of this utility model is in order to solve the defect of above-mentioned prior art, and a kind of simple in structure, with low cost, easy to operate, diverse in function is provided, and can meet many coupling In vitro cell experiment devices that simultaneously carry out many group experiments.
The purpose of this utility model can reach by taking following technical scheme:
Many coupling In vitro cell experiment devices is characterized in that: but comprise the container of constant control water temperature, with container, be complementary and surface be provided with a plurality of perforates blind flange, can realize the uniform magnetic stirring apparatus of distribution of water temperature and can place the sample pallet with the electromagnetic field generator of porous cell culture plate and/or single porous cell culture plate; The top and bottom of described container are respectively arranged with filler and dewatering outlet, and place, described dewatering outlet is provided with ball valve, but described blind flange is connected to the structure that the opened/closed container is formed on the top of container, and described magnetic stirring apparatus is arranged on the bottom of container; Described sample pallet is placed in container when experiment.
As a kind of preferred version, described container lower end also is provided with heating tube and, for placing the first thermometer hole of the first thermometer, described heating tube is connected with outside temperature regulating device respectively with the first thermometer, realizes the constant control water temperature.
As a kind of preferred version, the perforate that described blind flange surface arranges comprises air inlet port, production well, circuit jack, for placing barometric weather gage hole, for the second thermometer hole of placing the second thermometer and for placing hygroscopic wet bulb thermometer hole.
As a kind of preferred version, described electromagnetic field generator comprises one group of parallel Helmholtz coil, one group of parallel-plate electrode, dull and stereotyped pallet and power supply, and described parallel-plate electrode is placed on dull and stereotyped pallet, and described power supply bottom is provided with terminal stud; In the time of in electromagnetic field generator is placed in container, the wiring of described terminal stud is connected with the external power source control device by the circuit jack.
As a kind of preferred version, described sample pallet comprises that Multilayer Samples platform and at least three are for supporting the thread rack of sample table, described every layer of sample table is the irregular roundness shape that crops the part arc length, its surface is provided with at least three bracket holes that are complementary with thread rack, and described thread rack is through regulating the height with the fixed sample platform by hex-nut after bracket holes.
As a kind of preferred version, the surface of described every layer of sample table also is provided with a plurality of for placing the boring of centrifuge tube.
As a kind of preferred version, described container is the cylindrical straight tubular that insulating interlayer is housed, and conveniently the sample pallet is put into or is proposed, and improve the homogeneity of internal temperature, and it adopts stainless material to make, and can stablize pressurize.
As a kind of preferred version, be provided with rubber cradle between the top of described blind flange and container, to improve the sealing effectiveness of container.
The utility model has following beneficial effect with respect to prior art:
1, many coupling In vitro cell experiment devices of the present utility model can be realized many couplings of the field of force, magnetic field, electric field by electromagnetic field generator, also can realize the independent use of the field of force, magnetic field, electric field, can conveniently study more real biomechanics, the bioelectromagnetics Discussion of Mechanism in the molecular biology level.
2, many coupling In vitro cell experiment devices of the present utility model can be controlled the required atmosphere of cell cultures, temperature and humidity condition in container, the magnetic stirring apparatus that install bottom, can realize being evenly distributed of water temperature, the spatial distribution that has guaranteed the internal tank experimental temperature is even, can independently carry out In vitro cell experiment, without putting into specific cell culture incubator.
3, many coupling In vitro cell experiment devices of the present utility model are when experiment, the wiring of electromagnetic field generator can be connected with the external power source control device by the circuit jack, according to actual needs, utilize the external power source control device, can realize the cell culture environment under stationary magnetic field, pulsed magnetic field, steady electric field, constant pressure, but also be applicable to other, need the exert oneself research of other experiments such as chemosynthesis of electromagnetic field environment of temperature control.
4, many sample pallets that coupling In vitro cell experiment device is used of the present utility model, can regulate the height of sample table, thereby obtain suitable space, can conveniently carry out the cell sample of many group different experimental conditions is tested simultaneously, can meet the demand of lot of experiments, and sample table adopts the irregular roundness shape design that crops the part arc length, the circuit discharge of convenient electromagnetic generating unit, also can make the range of temperature of thermometer energy measuring vessel different heights, make environment control more science.
5, the structure of container that many coupling In vitro cell experiment devices of the present utility model are used is simple, with low cost, by stainless steel, made, have the advantages that height is withstand voltage, height is airtight, easily disinfect, and adopt Modular assembled to form, facilitate cleaning and sterilizing and change bad, old component.
The accompanying drawing explanation
The container Facad structure schematic diagram that Fig. 1 is many coupling In vitro cell experiment devices of the utility model.
The container plan structure schematic diagram that Fig. 2 is many coupling In vitro cell experiment devices of the utility model.
The sample pallet Facad structure schematic diagram that Fig. 3 is many coupling In vitro cell experiment devices of the utility model.
Sample table surface tissue schematic diagram in the sample pallet that Fig. 4 is many coupling In vitro cell experiment devices of the utility model.
Fig. 5 is the electromagnetic field generator structural representation with many of the utility model coupling In vitro cell experiment matched with devices.
Wherein, 1-container, 2-blind flange, the 3-magnetic stirring apparatus, 4-sample pallet, 5-filler, the 6-dewatering outlet, 7-heating tube, 8-the first thermometer hole, the 9-ball valve, 10-air inlet port, 11-production well, 12-circuit jack, 13-weather gage hole, 14-the second thermometer hole, 15-wet bulb thermometer hole, 16-rubber cradle, 17-sample table, the 18-thread rack, 19-bracket holes, 20-boring, the 21-hex-nut, 22-Helmholtz coil, 23-parallel-plate electrode, the dull and stereotyped pallet of 24-, the 25-power supply, 26-terminal stud, 27-porous cell culture plate.
Embodiment
Embodiment 1:
As shown in Figure 1-Figure 3, many of the present embodiment coupling In vitro cell experiment devices, the blind flange 2, magnetic stirring apparatus 3 and the sample pallet 4 that comprise container 1, be complementary with container 1.
As depicted in figs. 1 and 2, described container 1 is for being equipped with the cylindrical straight tubular of insulating interlayer, it adopts stainless material to make, can stablize pressurize, the upper end of described container 1 is provided with filler 5, lower end is provided with dewatering outlet 6, heating tube 7 and the first thermometer hole 8, the water larger by filler 5 injection specific heat capacities is as thermal insulation medium, 6 places, described dewatering outlet are provided with ball valve 9, the first thermometer hole 8 is placed with the first thermometer, and described heating tube 7 is connected with outside temperature regulating device respectively with the first thermometer; Described blind flange 2 is connected to the structure that but opened/closed container 1 is formed on the top of container 1, its surface is provided with air inlet port 10, production well 11, circuit jack 12, weather gage hole 13, the second thermometer hole 14 and wet bulb thermometer hole 15, described weather gage hole 13 is placed with weather gage, described the second thermometer hole 14 is placed with the second thermometer, described wet bulb thermometer hole 15 is placed with wet bulb thermometer, be provided with rubber cradle 16 between blind flange 2 and container 1 top, to improve the sealing effectiveness of container 1; Described magnetic stirring apparatus 3 is arranged on the bottom of container 1, can make the water generates eddy current of container 1 interlayer, even to guarantee the distribution of water temperature in container.
As shown in Figure 3 and Figure 4, described sample pallet 4 comprises four layers of sample table 17 and three thread racks 18, described every layer of sample table 17 is for cropping the irregular roundness shape of part arc length, its surface is provided with three bracket holes 19 and 16 borings 20, described bracket holes 19 is complementary with thread rack 18, described boring 20 can be placed the 15ml centrifuge tube corresponding with its diameter, and described thread rack 18 adopts stainless material to make; The height of every layer of sample table 17 is regulated and fixed to thread rack 18, through bracket holes 19, by hex-nut 21, and described every layer of sample table 17 can be placed with the electromagnetic field generator of porous cell culture plate and/or single porous cell culture plate.
As shown in Figure 5, described electromagnetic field generator comprises one group of parallel Helmholtz coil 22, one group of parallel-plate electrode 23, dull and stereotyped pallet 24 and power supply 25, described parallel-plate electrode 23 is placed on dull and stereotyped pallet 24, and described power supply 25 bottoms are provided with terminal stud 26; Be placed in container 1 when interior at electromagnetic field generator, the wiring of described terminal stud 26 is connected with the external power source control device by circuit jack 12.
As Figure 1-Figure 5, many coupling In vitro cell experiment processes of the present embodiment comprise the following steps:
1), by after container 1 sterilization and disinfection, in container 1, add a certain amount of deionized water to keep the humidity environment of cell cultures;
The porous cell culture plate 27 that 2) will be placed with cell sample is placed on 23 of the parallel-plate electrodes of electromagnetic field generator, makes porous cell culture plate 27 can load electromagnetic field;
3) electromagnetic field generator is placed on the first layer (from top to bottom) sample table 17 of sample pallet 4, then sample pallet 4 is placed in container 1, the wiring of terminal stud 26 is connected with the external power source control device by circuit jack 12, by blind flange 2 unopened containers 1;
4) by air inlet port 10 with rate of flow meter containing 5%CO
2medical mixed gas gas-holder be connected, then adjust pore 11, by weather gage, monitored, obtain stable air pressure;
5) closed ball valve 9 is closed dewatering outlet 6, makes container 1 in air-tight state, in filler 5, injects appropriate water, opens outside temperature regulating device, utilizes heating tube 7 heating, by the first thermometer, is monitored, and makes water temperature constant in preset temp A;
6) after outside temperature regulating device is opened for some time, by the interior gas temperature of the second thermometer measuring vessel 1, be actual temperature B, by magnetic stirring apparatus 3, control B=37 ℃;
7) after finishing, first turn off external power source control device, outside temperature regulating device and gas-holder pressurizing valve, then adjust pore 11 container 1 internal gas pressure is reduced, open blind flange 2 and take out sample pallets 4.
Embodiment 2:
Many the coupling In vitro cell experiment process principal features of the present embodiment are: step 3), regulate the hex-nut 21 of sample pallet 4, obtain suitable space, electromagnetic field generator is placed on the first layer (from top to bottom) sample table 17 of sample pallet 4, the single porous cell culture plate that is placed with cell sample is placed on the second layer sample table 17 of sample pallet 4 simultaneously, realize making the cell sample experiment of electricity consumption, magnetic, many couplings of power, also realized the cell sample experiment in the independent use field of force.All the other are with embodiment 1.
Embodiment 3:
Many the coupling In vitro cell experiment process principal features of the present embodiment are: step 3), regulate the hex-nut 21 of sample pallet 4, obtain suitable space, electromagnetic field generator is placed on the first layer (from top to bottom) sample table 17 of sample pallet 4, the single porous cell culture plate that is placed with cell sample is placed on the second layer sample table 17 of sample pallet 4 simultaneously, and the 15ml centrifuge tube that will be placed with cell sample is placed in the boring 20 of the 3rd layer of sample table 17 of sample pallet 4, experiment when realizing the various kinds of cell sample.All the other are with embodiment 1.
The above; it is only the utility model patent preferred embodiment; but the protection domain of the utility model patent is not limited to this; anyly be familiar with those skilled in the art in the disclosed scope of the utility model patent; be equal to replacement or changed according to technical scheme and the utility model patent design thereof of the utility model patent, all being belonged to the protection domain of the utility model patent.
Claims (8)
1. many coupling In vitro cell experiment devices is characterized in that: but comprise the container of constant control water temperature, with container, be complementary and surface be provided with a plurality of perforates blind flange, can realize the uniform magnetic stirring apparatus of distribution of water temperature and can place the sample pallet with the electromagnetic field generator of porous cell culture plate and/or single porous cell culture plate; The top and bottom of described container are respectively arranged with filler and dewatering outlet, and place, described dewatering outlet is provided with ball valve, but described blind flange is connected to the structure that the opened/closed container is formed on the top of container, and described magnetic stirring apparatus is arranged on the bottom of container; Described sample pallet is placed in container when experiment.
2. many coupling In vitro cell experiment devices according to claim 1, it is characterized in that: described container lower end also is provided with heating tube and, for placing the first thermometer hole of the first thermometer, described heating tube is connected with outside temperature regulating device respectively with the first thermometer.
3. many coupling In vitro cell experiment devices according to claim 2 is characterized in that: the perforate that described blind flange surface arranges comprises air inlet port, production well, circuit jack, for placing barometric weather gage hole, for the second thermometer hole of placing the second thermometer and for placing hygroscopic wet bulb thermometer hole.
4. many coupling In vitro cell experiment devices according to claim 3, it is characterized in that: described electromagnetic field generator comprises one group of parallel Helmholtz coil, one group of parallel-plate electrode, dull and stereotyped pallet and power supply, described parallel-plate electrode is placed on dull and stereotyped pallet, and described power supply bottom is provided with terminal stud; In the time of in electromagnetic field generator is placed in container, the wiring of described terminal stud is connected with the external power source control device by the circuit jack.
5. many coupling In vitro cell experiment devices according to claim 1, it is characterized in that: described sample pallet comprises that Multilayer Samples platform and at least three are for supporting the thread rack of sample table, described every layer of sample table is the irregular roundness shape that crops the part arc length, its surface is provided with at least three bracket holes that are complementary with thread rack, and described thread rack is through regulating the height with the fixed sample platform by hex-nut after bracket holes.
6. many coupling In vitro cell experiment devices according to claim 5, it is characterized in that: the surface of described every layer of sample table also is provided with a plurality of for placing the boring of centrifuge tube.
7. according to described many coupling In vitro cell experiment devices of claim 1-6 any one, it is characterized in that: described container is the cylindrical straight tubular that insulating interlayer is housed, and it adopts stainless material to make.
8. according to described many coupling In vitro cell experiment devices of claim 1-6 any one, it is characterized in that: between the top of described blind flange and container, be provided with rubber cradle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201320426926 CN203360449U (en) | 2013-07-17 | 2013-07-17 | Multi-field coupling in vitro cell experiment device |
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| CN 201320426926 CN203360449U (en) | 2013-07-17 | 2013-07-17 | Multi-field coupling in vitro cell experiment device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105505773A (en) * | 2015-12-28 | 2016-04-20 | 北京航空航天大学 | Magnetic-electric stimulation cell culture device |
| CN108130275A (en) * | 2018-02-01 | 2018-06-08 | 三峡大学 | A kind of Tissue Culture Dish for applying three-dimensional magnetic field |
-
2013
- 2013-07-17 CN CN 201320426926 patent/CN203360449U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105505773A (en) * | 2015-12-28 | 2016-04-20 | 北京航空航天大学 | Magnetic-electric stimulation cell culture device |
| CN108130275A (en) * | 2018-02-01 | 2018-06-08 | 三峡大学 | A kind of Tissue Culture Dish for applying three-dimensional magnetic field |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131225 Termination date: 20160717 |
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| CF01 | Termination of patent right due to non-payment of annual fee |