CN217103916U - Universal horizontal support of cell culture bottle - Google Patents
Universal horizontal support of cell culture bottle Download PDFInfo
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- CN217103916U CN217103916U CN202221040097.5U CN202221040097U CN217103916U CN 217103916 U CN217103916 U CN 217103916U CN 202221040097 U CN202221040097 U CN 202221040097U CN 217103916 U CN217103916 U CN 217103916U
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- board
- cell culture
- blake bottle
- adjusting rod
- bottle
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Abstract
In order to solve the quality problem that liquid level slope leads to in the blake bottle, the utility model provides an universal horizontal stand of cell culture bottle, a serial communication port, place board, base including the blake bottle, the blake bottle is placed the board and is frame construction, the blake bottle is placed and is provided with the bubble spirit level on the board, the blake bottle is placed and is provided with the spacing hole that the position corresponds the distribution on board and the base, it is fixed continuous through the connecting piece between two spacing holes that the position corresponds the distribution, judge through the horizontal bubble spirit level whether the blake bottle is placed the board and is placed the position level, if not level, accessible adjusting connection axle height, make the blake bottle place the board and reach the level, then place the blake bottle and place and carry out cell culture on the board is placed to the blake bottle, effectively solve the local too dense or too thin problem of cell culture because of the unsmooth cause of baffle in the cell culture box.
Description
Technical Field
The utility model relates to a cell culture device technical field, in particular to universal horizontal stand of cell culture bottle.
Background
Cell culture, refers to a method of simulating the in vivo environment in vitro, allowing cells to grow, reproduce and maintain the main structure and function. The cell culture technique can obtain a target cell line by subjecting a single cell to mass culture to simple single cell cloning or directed differentiation into a target cell, and can also study cell signal transduction, cell anabolism, cell growth and proliferation, and the like.
In the process of cell culture, suspension culture and adherent culture can be divided according to the growth mode of cells in a culture container. As the name implies, suspension culture refers to the suspension of cells in a cell culture fluid during the culture process, such as most immune cells and a part of tumor cells, and the cell culture container can be a cell culture bottle or a culture bag. Adherent culture refers to the growth of cells attached to the surface of a culture container in the culture process, the growth of the cells needs to be interdependent, and the cells need to be attached to the surface of a non-chemical substance (glass, plastic or other inactive substances) for growth, survival and maintenance, and finally contact inhibition occurs when an attachment surface grows to a monolayer and is paved on a plane. Most animal cells, including cells of non-lymphoid tissues and cells of a plurality of heteroploid systems belong to the type, an adherent cell culture vessel is mainly a cell culture bottle, when the adherent cells are cultured, primary isolated or frozen seed cells are firstly suspended in a cell culture medium according to a certain density, then are placed in a culture bottle special for the adherent cells, and finally the culture bottle is horizontally placed on a partition board in a cell culture box and is statically cultured under a certain temperature and carbon dioxide concentration. During the first hours of culture, cells suspended in the medium will settle freely and attach to the bottom of the flask, and then grow and expand continuously. After several days of culture, generally when the cells cover about 80% of the culture flask, enzymolysis digestion is carried out, and the cells are collected and frozen or continuously subcultured and expanded. Generally, the higher the initial inoculation density is, the faster the cells grow, but the culture time is not easy to control, the cells may grow too densely in a short time, and the cells will age and differentiate. If the cell seeding is too thin, the cells grow slowly or even cannot grow.
The working area of the cell incubator is a square cavity, a plurality of horizontal clapboards are arranged in the cell incubator, one or more culture bottles are piled up and placed on the clapboards for culture when in use, and usually 4-9 piles of culture bottles can be placed on one incubator clapboard according to different areas of the bottoms of the culture bottles. However, the problems of quality of the incubator, depreciation of equipment, excessive bearing and the like often cause the problems of deformation and inclination of the partition plate, so that the liquid level of the culture solution in the culture bottle is inclined during cell culture, the density of cells settled to the bottom of the bottle at the beginning is uneven, the subsequent cell amplification speed also generates great difference, partial cells are too dense after the culture, partial cells are too thin, and the overall quality of the cells is greatly reduced. In industrial production, in order to control production cost, the using amount of culture solution is generally less, and the bottle carrying rate of an incubator is generally higher, so that the situation is more obvious.
Disclosure of Invention
In order to solve the problem, the utility model provides a cell culture bottle universal horizontal bracket, the technical scheme of adoption as follows:
the utility model provides a universal horizontal stand of cell culture bottle, its characterized in that places board, base including the blake bottle, the blake bottle is placed the board and is frame construction, and the blake bottle is placed and is provided with the bubble spirit level on the board, and the blake bottle is placed and is provided with the spacing hole that the position corresponds the distribution on board and the base, and the position corresponds to and links to each other through the connecting piece is fixed between two spacing holes that distribute.
Preferably, the connecting piece comprises a fixed shaft and a connecting shaft with adjustable length, one end of the culture bottle placing plate is connected with the base through the fixed shaft, and the other end of the culture bottle placing plate is connected with the base through the connecting shaft.
Preferably, the connecting shaft comprises an adjusting knob, a first adjusting rod and a second adjusting rod, wherein an internal thread is arranged in the adjusting knob, the first adjusting rod and the second adjusting rod are respectively in threaded connection with the upper portion and the lower portion of the adjusting knob, external threads matched with the adjusting knob are arranged on the first adjusting rod and the second adjusting rod, and the rotating directions of the threads on the first adjusting rod and the second adjusting rod are opposite.
Preferably, the upper part of the first adjusting rod and the lower part of the second adjusting rod are both provided with a clamping groove matched with the limiting hole.
The beneficial effects of the utility model reside in that: judge through the horizontal bubble appearance that the blake bottle is placed the board and is placed whether level in position, if not level, the accessible is adjusted connecting axle height, makes the blake bottle place the board and reach the level, then places the cell culture bottle and place and carry out cell culture on the board is placed to the blake bottle, effectively solves the local too close or too rare problem of cell culture because of the unhomogeneous cell culture that causes of baffle in the cell culture case.
Drawings
FIG. 1 is a schematic view of the structure of the present invention
FIG. 2 is an exploded view of the structure of the present invention
FIG. 3 is a schematic view of a length-adjustable connecting shaft
FIG. 4 is a schematic view of the internal structure of FIG. 3
The culture flask comprises a culture flask placing plate 1, a 2-connecting shaft, 201-an adjusting knob, 202-a first adjusting rod, 203-a second adjusting rod, 204-a clamping groove, 3-a base, 4-a limiting hole, 5-a horizontal bubble instrument and 6-a fixing shaft.
Detailed Description
Exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. While exemplary embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.
In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description of the present invention, and do not indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
The universal horizontal bracket for the cell culture bottle as shown in the figures 1-4 comprises a culture bottle placing plate 1 and a base 3, wherein the culture bottle placing plate 1 is of a rectangular frame structure, a horizontal bubble instrument 5 is fixedly connected on the culture bottle placing plate 1, the base 3 is of a semi-elliptical frame structure as a whole, limiting holes 4 which are distributed in a penetrating way from top to bottom are arranged at the front and back sides of the left end and the right end of the culture bottle placing plate 1 and the base 3, the limiting holes 4 on the culture bottle placing plate 1 and the base 3 are correspondingly distributed, a connecting shaft 2 is connected between the two limiting holes which are distributed correspondingly, the left side of the culture bottle placing plate 1 and the left side of the base 3 are fixedly connected through an integrally formed fixed shaft 6, the right side of the culture bottle placing plate 1 is fixedly connected through a connecting shaft 2 with adjustable length, the connecting shaft 2 is composed of an adjusting knob 201, a first adjusting rod 202 and a second adjusting rod 203, threaded holes which are distributed in a penetrating way from top to bottom are arranged inside the adjusting knob 201, the outer surfaces of the lower part of the first adjusting rod 202 and the upper part of the second adjusting rod 203 are provided with external threads matched with threaded holes of the adjusting knob 201, the rotating directions of the external threads on the first adjusting rod 202 and the second adjusting rod 203 are opposite, in addition, annular clamping grooves 204 are arranged on the lower parts of the upper part of the first adjusting rod 202 and the second adjusting rod 203 and the upper part and the lower part of the fixed shaft 6, and the fixed shaft 6 and the adjusting shaft 2 are clamped with the limiting hole 4 through the clamping grooves 204.
During the use, place the device in the incubator on certain position on certain baffle earlier, judge 1 through horizontal bubble appearance 5 and be in horizontal plane position, if not flat, then rotate adjust knob 201 with the length of adjustment regulating spindle 2, observe horizontal bubble appearance 5 simultaneously, place board 1 and be in horizontal plane position until the blake bottle, then pile up the cell culture bottle one or several that will treat the cultivation and place on the blake bottle places board 1, confirm again through horizontal bubble appearance 5 that the blake bottle places board 1 and be in horizontal position, if not flat can finely tune two regulating spindles 2 again, place board 1 and be in horizontal plane position until the blake bottle, if alternate the blake bottle position in the process of cultivation, need to use this device again and adjust the blake bottle level.
The above description is only a preferred embodiment of the present invention, and it should be noted that: for those skilled in the art, without departing from the principle of the present invention, several improvements can be made, and these improvements should also be regarded as the protection scope of the present invention.
Claims (4)
1. The utility model provides a universal horizontal stand of cell culture bottle, its characterized in that places board, base including the blake bottle, the blake bottle is placed the board and is frame construction, and the blake bottle is placed and is provided with the bubble spirit level on the board, and the blake bottle is placed and is provided with the spacing hole that the position corresponds the distribution on board and the base, and the position corresponds to and links to each other through the connecting piece is fixed between two spacing holes that distribute.
2. The universal horizontal support for cell culture bottles of claim 1, wherein the connecting piece comprises a fixed shaft and a connecting shaft with adjustable length, one end of the culture bottle placing plate is connected with the base through the fixed shaft, and the other end of the culture bottle placing plate is connected with the base through the connecting shaft.
3. The cell culture bottle universal horizontal bracket as claimed in claim 2, wherein the connecting shaft is composed of an adjusting knob, a first adjusting rod and a second adjusting rod, wherein the adjusting knob is internally provided with internal threads, the first adjusting rod and the second adjusting rod are respectively in threaded connection with the upper part and the lower part of the adjusting knob, the first adjusting rod and the second adjusting rod are provided with external threads matched with the adjusting knob, and the threads on the first adjusting rod and the second adjusting rod are in opposite directions.
4. The universal horizontal bracket for cell culture bottles of claim 3, wherein the upper part of the first adjusting rod and the lower part of the second adjusting rod are provided with clamping grooves matched with the limiting holes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221040097.5U CN217103916U (en) | 2022-05-05 | 2022-05-05 | Universal horizontal support of cell culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221040097.5U CN217103916U (en) | 2022-05-05 | 2022-05-05 | Universal horizontal support of cell culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217103916U true CN217103916U (en) | 2022-08-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221040097.5U Active CN217103916U (en) | 2022-05-05 | 2022-05-05 | Universal horizontal support of cell culture bottle |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117070451A (en) * | 2023-10-13 | 2023-11-17 | 北京中卫医正科技有限公司 | Subculture method of mesenchymal stem cells |
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2022
- 2022-05-05 CN CN202221040097.5U patent/CN217103916U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117070451A (en) * | 2023-10-13 | 2023-11-17 | 北京中卫医正科技有限公司 | Subculture method of mesenchymal stem cells |
| CN117070451B (en) * | 2023-10-13 | 2024-01-30 | 北京中卫医正科技有限公司 | Subculture method of mesenchymal stem cells |
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