CN207845661U - A kind of lymphocyte layering culture bottle - Google Patents
A kind of lymphocyte layering culture bottle Download PDFInfo
- Publication number
- CN207845661U CN207845661U CN201721922141.4U CN201721922141U CN207845661U CN 207845661 U CN207845661 U CN 207845661U CN 201721922141 U CN201721922141 U CN 201721922141U CN 207845661 U CN207845661 U CN 207845661U
- Authority
- CN
- China
- Prior art keywords
- sieve
- aperture
- lymphocyte
- microns
- bottle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 210000004698 lymphocyte Anatomy 0.000 title claims abstract description 29
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 abstract description 11
- 210000003743 erythrocyte Anatomy 0.000 abstract description 5
- 238000011109 contamination Methods 0.000 abstract description 4
- 230000012010 growth Effects 0.000 abstract description 4
- 239000000463 material Substances 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 210000001165 lymph node Anatomy 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The utility model discloses a kind of lymphocytes to be layered culture bottle, including 3 layers of sieve being horizontally set in bottle, and the aperture of upper screen is 11 microns, the aperture of middle level sieve is 9 microns, the aperture of lower screen is 7 microns；Bottom of bottle is 0.2 0.3cm with the distance between sieve and two neighboring sieve.By adding the different sieve in aperture in Tissue Culture Flask, lymphocyte can be separately cultured in same system, advantageously reduce the growth that mutually squeezes and influence lymphocyte of the red blood cell between lymphocyte and lymphocyte, it is time saving and energy saving, and consumptive material can be saved, cost is reduced, while being conducive to the cell contamination problem for avoiding cumbersome operation from bringing.
Description
Technical field
The utility model is related to field of cell culture more particularly to a kind of lymphocyte to be layered culture bottle.
Background technology
In anticoagulated whole blood, the size of red blood cell is 6~8.5 μm, is mostly 7 μm, lymphocyte can be divided into small by size
Lymphocyte (5~8 μm of diameter), medium size lymphocyte (9~12 μm) and large lymphocyte (13~20 μm) are only deposited in normal blood
In small lymphocyte and medium size lymphocyte, large lymphocyte sees the centrum germinativum of spleen and lymph node.In the prior art, different big
Small lymphocyte and red blood cell mix culture, are also easy to produce mutually extruding and influence the growth of lymphocyte.
Under normal conditions, in order to be separately cultured, increase culture efficiency needs to centrifuge in cell liquid, red thin to remove
Then born of the same parents carry out lymphocyte culture, not only complex steps, but also waste a large amount of consumptive materials, also increase the possibility of cell contamination
Property.
Invention content
The purpose of this utility model is that in view of the deficiencies of the prior art, providing a kind of lymphocyte layering culture bottle.
The purpose of this utility model is achieved through the following technical solutions：A kind of lymphocyte layering culture bottle, including
3 layers of sieve being horizontally set in bottle, the aperture of upper screen is 11 microns, the aperture of middle level sieve is 9 microns, lower screen
Aperture be 7 microns；Bottom of bottle is 0.2-0.3cm with the distance between sieve and two neighboring sieve.
Further, the twine pan coating for constituting the sieve has anti-CD monoclonal antibodies layer.
Further, the bottle is connected through a screw thread and is formed by top, middle and upper part, middle and lower part, four part of lower part, described
Top, middle and upper part, middle and lower part bottom surface be respectively upper screen, the middle level sieve that aperture is 9 microns, hole that aperture is 11 microns
The lower screen that diameter is 7 microns, bottom face are bottle bottom surface.
The beneficial effects of the utility model are：The sieve different by adding aperture in Tissue Culture Flask, by lymph
Cell is separately cultured in same system, and it is mutual between lymphocyte and lymphocyte to advantageously reduce red blood cell
The growth of lymphocyte is squeezed and influences, it is time saving and energy saving, and consumptive material can be saved, cost is reduced, while being conducive to avoid numerous
The cell contamination problem that trivial operation is brought.
Description of the drawings
Fig. 1 is the structural schematic diagram of the utility model；
Fig. 2 is the schematic diagram of the twine of sieve；
In figure, top 1, middle and upper part 2, middle and lower part 3, lower part 4, upper screen 11, middle level sieve 12, lower screen 13, net
Silk 21, anti-CD49d McAb layer 22.
Specific implementation mode
As shown in Figure 1, a kind of lymphocyte is layered culture bottle, including 3 layers of sieve being horizontally set in bottle, upper sieve
The aperture of net 11 is 11 microns, the aperture of middle level sieve 12 is 9 microns, the aperture of lower screen 13 is 7 microns；Two neighboring sieve
The distance between net is 1cm.Bottle is divided into top 1, middle and upper part 2, middle and lower part 3,4 four part of lower part by three-layer screen；In use,
Cell liquid is added in cell bottle, the means such as washes away, rock by culture solution so that different size of cell has respectively entered
Be separately cultured in different layers, advantageously reduce red blood cell between lymphocyte and lymphocyte it is mutual extruding and shadow
The growth of lymphocyte is rung, while being conducive to the cell contamination problem for avoiding cumbersome operation from bringing.
As shown in Fig. 2, 21 pan coating of twine for constituting the sieve has anti-CD49d McAb layer 22.Utilize T lymphocyte tables
Stimulant of the monoclonal antibody of face CD3 molecules as cell growth, can mass propgation lymphocyte, prepare it for chromosome
With.
Preferably, the bottle is connected through a screw thread and is formed by top 1, middle and upper part 2, middle and lower part 3,4 four part of lower part,
The top 1, middle and upper part 2, middle and lower part 3 bottom surface be respectively upper screen 11, aperture that aperture is 11 microns be in 9 microns
Layer sieve 12, the lower screen 13 that aperture is 7 microns, bottom face are bottle bottom surface.After the completion of cell culture, top is first extracted
1 cell liquid after then detaching top, takes the cell liquid of middle and upper part 2, then takes the cell of middle and lower part 3, lower part 4 successively again
Liquid.Or sieve is directly removed, carry out chromosome preparation.
Claims (3)
1. a kind of lymphocyte is layered culture bottle, which is characterized in that including 3 layers of sieve being horizontally set in bottle, upper sieve
The aperture of net (11) is 11 microns, the aperture of middle level sieve (12) is 9 microns, the aperture of lower screen (13) is 7 microns；Bottom of bottle
The distance between lower screen (13) and two neighboring sieve are 0.2-0.3cm.
2. culture bottle according to claim 1, which is characterized in that constitute the sieve twine (21) pan coating have it is anti-
CD3 monoclonal antibodies layer (22).
3. culture bottle according to claim 1, which is characterized in that the bottle is by top (1), middle and upper part (2), middle and lower part
(3), (4) four part of lower part is connected through a screw thread composition, the top (1), middle and upper part (2), middle and lower part (3) bottom surface be respectively
Upper screen (11) that aperture is 11 microns, the middle level sieve (12) that aperture is 9 microns, the lower screen that aperture is 7 microns
(13), bottom face is bottle bottom surface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721922141.4U CN207845661U (en) | 2017-12-30 | 2017-12-30 | A kind of lymphocyte layering culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721922141.4U CN207845661U (en) | 2017-12-30 | 2017-12-30 | A kind of lymphocyte layering culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207845661U true CN207845661U (en) | 2018-09-11 |
Family
ID=63416605
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721922141.4U Expired - Fee Related CN207845661U (en) | 2017-12-30 | 2017-12-30 | A kind of lymphocyte layering culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207845661U (en) |
-
2017
- 2017-12-30 CN CN201721922141.4U patent/CN207845661U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180911 Termination date: 20191230 |