CN204679302U - A kind of submicrosample original position filtering and sampling device - Google Patents
A kind of submicrosample original position filtering and sampling device Download PDFInfo
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- CN204679302U CN204679302U CN201520414918.0U CN201520414918U CN204679302U CN 204679302 U CN204679302 U CN 204679302U CN 201520414918 U CN201520414918 U CN 201520414918U CN 204679302 U CN204679302 U CN 204679302U
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Abstract
The utility model relates to a kind of submicrosample original position filtering and sampling device, it comprises porous plate (6), has the filter frame (2) of some shrinkage pools (7) and sampling probe (5), each Kong Weiyi sample cell (3) on wherein said porous plate (6); Described filter frame (2) upper limb can be stuck in that described porous plate (6) is upper and the shrinkage pool (7) of described filter frame (2) can enter in described sample cell (3), and shrinkage pool (7) bottom of wherein said filter frame (2) is provided with filter screen (1).Submicrosample original position filtering and sampling apparatus structure of the present utility model is simple, easy to operate, can without the need to artificial filter, little to sample loss, has very strong applicability and operability.
Description
Technical field
The utility model belongs to filter plant technical field, is specifically related to a kind of submicrosample original position filtering and sampling device.
Background technology
Flow cytometry is a kind of multinomial physics and biological characteristics of the individual cells that can detect in quick streamlined flow state, and is analyzed quantitative technology.Be characterized in carrying out many important parameters such as quantitative measurement cell DNA content, cell volume, protein content, enzymatic activity, cell-membrane receptor and surface antigen by Fast Measurement Ku Erte resistance, fluorescence, light scattering and light absorption.According to these parameters by cell of different nature separately, to obtain the pure cell colony for biology and medical research.
Because flow cytometer is generally will analyze unicellular, and cell easily forms cell mass when digestion, dyeing, the multi task process such as centrifugal, fixing, and the existence of cell mass can affect experimental result and block machine.The Normal practice in current laboratory before loading, gets one piece of nylon wire filter sample, thus the cell mass removed in suspension sample and bulk impurity.
Along with the development of streaming technology, most of flow cytometer has been equipped with high flux orifice plate automatic sample handling system can carry out rapid, full automatic 96 hole micro-samplings, but because the sample in 96 orifice plates is comparatively micro-, usually can not more than 200 μ l, if adopt traditional filter screen to filter each sample, cell sample loss is serious, may cause test cell quantity very little, and operation element amount is large, is unfavorable for the carrying out that high flux detects.
Utility model content
The purpose of this utility model is to overcome the deficiencies in the prior art, provide a kind of structure simple, easy and simple to handle, without the need to artificial filter, the submicrosample original position filtering and sampling device little to sample loss.
The purpose of this utility model is achieved by the following technical programs:
A kind of submicrosample original position filtering and sampling device, it comprises porous plate 6, has filter frame 2 and the sampling probe 5 of some shrinkage pools 7, each Kong Weiyi sample cell 3 on wherein said porous plate 6; Described filter frame 2 upper limb can be stuck on described porous plate 6 and the shrinkage pool 7 of described filter frame 2 can enter in described sample cell 3, is provided with filter screen 1 bottom the shrinkage pool 7 of wherein said filter frame 2.
In preferred embodiments, described porous plate 6 is 96 orifice plates.
In preferred embodiments, described filter frame 2 has the shrinkage pool 7 identical with the hole count of described porous plate 6.
In preferred embodiments, described sampling probe 5 is flow cytometer sample introduction needle.
In preferred embodiments, described filter screen 1 is 400 order nylon wires.
In preferred embodiments, the material of described filter frame 2 is polypropylene.
Wherein, described submicrosample refers to that sample volume is less than 500 microlitres, is preferably less than 300 microlitres, is more preferably less than 200 microlitres, is still more preferably less than 100 microlitres, then is more preferably less than 50 microlitres.
The using method of this submicrosample original position filtering and sampling device is: when individual cells for extracting in cell culture, cell chulture is carried out in the sample cell 3 of described porous plate 6, after having cultivated, described filter frame 2 to be placed on described porous plate 6 and to make the shrinkage pool 7 of described filter frame 2 enter into described sample cell 3, now described sampling probe 5 to be inserted in the shrinkage pool 7 of described filter frame 2 and by swabbing action, the cell sample in described sample cell 3 to be filtered by the filter screen 1 be positioned at bottom described shrinkage pool 7, under the swabbing action of described sampling probe 5, individual cells can pass through described filter screen 1, cell mass and other large particulate matters then cannot pass through, stay outside described filter screen 1.When this submicrosample original position filtering and sampling device is used for flow cytomery, described sampling probe 5 is flow cytometer sample introduction needle, this submicrosample original position filtering and sampling device is positioned in flow cytometer orifice plate automatic sample handling system, is particularly suitable for high-throughout micronucleus cell sample preparation.This submicrosample original position filtering and sampling device also can be used for filtering other samples, to be positioned over by sample to be filtered in described sample cell 3 and to adjust the order number of described filter screen 1 according to the size of the Objective extraction thing after filtering.
The utility model achieves following beneficial effect:
1, submicrosample original position filtering and sampling apparatus structure of the present utility model is simple, easy to operate, can without the need to artificial filter, little to cell sample loss, and the original position that can realize submicrosample is filtered, and has very strong applicability and operability.
2, submicrosample original position filtering and sampling device of the present utility model is particularly suitable for the sample preparation of flow cytometer high flux orifice plate automatic sample handling system, the original position that can realize batch sample is filtered and direct injected, thus improves the detection efficiency of flow cytometry.
Accompanying drawing illustrates:
Fig. 1 is submicrosample original position filtering and sampling device use-pattern schematic diagram of the present utility model.
Fig. 2 is the submicrosample original position filtering and sampling device vertical view in the utility model embodiment 1.
Wherein, each Reference numeral has following implication:
1. filter screen; 2. filter frame; 3. sample cell; 4. cell sample; 5. sampling probe; 6. porous plate; 7. shrinkage pool.
Embodiment
Below in conjunction with embodiment and accompanying drawing, the utility model is further elaborated, but should be understood that explanation is only illustrative, and not restrictive.
Embodiment 1
The present embodiment relates to a kind of few cells filtrator being exclusively used in flow cytometer, as shown in Figure 1, it comprises 96 orifice plates 6, has the filter frame 2 of 96 shrinkage pools 7 and flow cytometer sample introduction needle 5, each Kong Weiyi sample cell 3 on wherein said 96 orifice plates 6; Described filter frame 2 upper limb can be stuck on described porous plate 6 and the shrinkage pool 7 of described filter frame 2 can enter in described sample cell 3, is provided with filter screen 1 bottom the shrinkage pool 7 of wherein said filter frame 2.Described filter screen 1 is 400 order nylon wires.The material of described filter frame 2 is polypropylene.
During use, cell chulture is carried out in the sample cell 3 of described 96 orifice plates 6, after cultivation completes when needs filter to extract individual cells, described filter frame 2 to be placed on described 96 orifice plates 66 and to make the shrinkage pool 7 of described filter frame 2 enter into described sample cell 3, now described flow cytometer sample introduction needle 5 to be inserted in the shrinkage pool 7 of described filter frame 2 and by swabbing action, the cell sample in described sample cell 3 to be filtered by the filter screen 1 be positioned at bottom described shrinkage pool 7, under the swabbing action of described flow cytometer sample introduction needle 5, individual cells can pass through described filter screen 1, cell mass and other large particulate matters then cannot by staying outside described filter screen 1.This flow cytometer few cells filtrator is positioned in flow cytometer orifice plate automatic sample handling system, is particularly suitable for high-throughout micronucleus cell sample preparation.
Claims (7)
1. a submicrosample original position filtering and sampling device, it is characterized in that, it comprises porous plate (6), has the filter frame (2) of some shrinkage pools (7) and sampling probe (5), each Kong Weiyi sample cell (3) on wherein said porous plate (6); Described filter frame (2) upper limb can be stuck in that described porous plate (6) is upper and the shrinkage pool (7) of described filter frame (2) can enter in described sample cell (3), and shrinkage pool (7) bottom of wherein said filter frame (2) is provided with filter screen (1).
2. submicrosample original position filtering and sampling device according to claim 1, it is characterized in that, described porous plate (6) is 96 orifice plates.
3. submicrosample original position filtering and sampling device according to claim 1, is characterized in that, described filter frame (2) has the shrinkage pool (7) identical with the hole count of described porous plate (6).
4. submicrosample original position filtering and sampling device according to claim 1, is characterized in that, described sampling probe (5) is flow cytometer sample introduction needle.
5. submicrosample original position filtering and sampling device according to claim 1, is characterized in that, described filter screen (1) is 400 order nylon wires.
6. submicrosample original position filtering and sampling device according to claim 1, is characterized in that, the material of described filter frame (2) is polypropylene.
7. submicrosample original position filtering and sampling device according to claim 1, it is characterized in that, described submicrosample refers to that sample volume is less than 500 microlitres.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520414918.0U CN204679302U (en) | 2015-06-16 | 2015-06-16 | A kind of submicrosample original position filtering and sampling device |
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| CN201520414918.0U CN204679302U (en) | 2015-06-16 | 2015-06-16 | A kind of submicrosample original position filtering and sampling device |
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| CN204679302U true CN204679302U (en) | 2015-09-30 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104865100A (en) * | 2015-06-16 | 2015-08-26 | 云南中烟工业有限责任公司 | In-situ filtering and sampling device for ultramicro-samples |
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2015
- 2015-06-16 CN CN201520414918.0U patent/CN204679302U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104865100A (en) * | 2015-06-16 | 2015-08-26 | 云南中烟工业有限责任公司 | In-situ filtering and sampling device for ultramicro-samples |
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