CN206666547U - A kind of biochip for being used to screen rare cell in positioning and detection blood - Google Patents
A kind of biochip for being used to screen rare cell in positioning and detection blood Download PDFInfo
- Publication number
- CN206666547U CN206666547U CN201720371980.5U CN201720371980U CN206666547U CN 206666547 U CN206666547 U CN 206666547U CN 201720371980 U CN201720371980 U CN 201720371980U CN 206666547 U CN206666547 U CN 206666547U
- Authority
- CN
- China
- Prior art keywords
- layer
- capture
- microfluidic channel
- biochip
- positioning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The utility model discloses a kind of biochip for being used to screen rare cell in positioning and detection blood, including basic unit and microfluidic channel layer, microfluidic channel layer is close to be arranged in basic unit, the upper surface of microfluidic channel layer is provided with capture groove, the bottom land of capture groove is provided with multiple enrichment holes, the depth in enrichment hole is 15~50 microns, the upper surface of microfluidic channel layer is provided with signal amplification layer, opening position corresponding with capture groove is provided with nano gold layer on the upper surface of signal amplification layer, it is provided with through nano gold layer and signal amplification layer into passage and passing away, connected respectively with capture groove into passage and passing away；Advantage is energy efficient capture circulating tumor cell, does not damage circulating tumor cell and facilitate subsequent detection.
Description
Technical field
A kind of biochip is the utility model is related to, more particularly to it is a kind of rare in positioning and detection blood for screening
The biochip of cell.
Background technology
In recent years, CTC (Circulating Tumor Cell：Circulating tumor cell) research obtained it is increasing
Concern, many research reports have confirmed CTC in the early diagnosis of metastases, curative effect monitoring, individualized treatment and explored swollen
Tumor metastasis mechanism etc. has potential value, but contents of the CTC in the circulatory system is extremely low, often about 10,000,000
One or several CTC can be just found in normal plasma cell, wants effectively to be enriched with these cells extremely difficult, it is necessary to by special
Biochip and sensitive detection device, this turns into the Major Difficulties for limiting its clinically relevant application.
The sorting enrichment method to CTC is broadly divided into biological method and the major class of physics method two at present.Biology side
Method refers to the specific antigen-antibody absorption of biomarker progress for selecting specific antibody to cell surface expression, so as to
Active sorting enrichment aim cell or specificity remove cell and albumen unless beyond aim cell, only retain aim cell.Its
Biggest advantage is high specific, can efficient identification CTC, its shortcoming mainly has：1st, due to antigen-antibody suction-operated not
It can be carried out under high flow velocities, therefore limit its flow velocity, so as to more time-consuming, flux is low；2nd, antigen-antibody reaction can influence
Cytoactive, cause the antigen (surface marker) of cell surface to be expressed and do not know；3rd, antibody reagent price is more high.
Physical method refers to being based on intercellular thing using external force field (such as magnetic field, electric field, fluid field, ultrasonic wave etc.)
Reason property difference (size, morphotropism, density, dielectricity etc.) is separated.Such as in Application No. 201210477243.5
State's utility model patent application discloses " a kind of biochip for being used to screen rare cell in blood ", and its structure is such as
Under：Including the reeded thin slice of tool and slide, the groove on thin slice is close to described load glass towards slide and by thin slice
On piece, make to form a closed microfluidic channel between thin slice and slide；Offered on described thin slice described in connection
The fluid hole and inlet opening of microfluidic channel.It is put into magnetic nano-particle in blood sample, and magnetic nano-particle absorption exists
On target cell, the blood sample that has then been labeled rare cell flows through microfluidic channel, being attached with blood sample
The rare cell of magnetic nano-particle is placed on the gradient magnetic guiding that the magnetic below biochip is sent, and waits collection work
After the completion of work, slide is separated with thin slice, subsequent analysis is carried out to the rare cell on slide.The biochip of the structure
Have the disadvantage that in use：1st, free nano-particle and labeled nano-particle tumour cell are often adsorbed
And an a small range on slide is gathered in, this aggregation may be disturbed in identification process or even damage tumour is thin
Born of the same parents；2nd, the target nano-particle tumour cell of mark rolls in weak magnetic field areas, in the case where not captured by magnetic
Easily escaped out of microfluidic channel.
The content of the invention
Main purpose of the present utility model is to provide a kind of energy efficient capture circulating tumor cell, does not damage circulating tumor
Cell and the biochip for being used to screen rare cell in positioning and detection blood for facilitating subsequent detection.
To achieve the above objectives, the technical solution adopted in the utility model is：One kind is used to screen positioning and detection blood
The biochip of middle rare cell, including basic unit and microfluidic channel layer, described microfluidic channel layer are close to be arranged on described
Basic unit on, the upper surface of described microfluidic channel layer is provided with capture groove, and the bottom land of described capture groove is provided with multiple
Enrichment hole, the depth in described enrichment hole is 15~50 microns, and the upper surface of described microfluidic channel layer is provided with letter
Number amplification layer, opening position corresponding with described capture groove is provided with nano gold layer on the upper surface of described signal amplification layer,
It is provided with through described nano gold layer and described signal amplification layer into passage and passing away, described enters passage
Connected respectively with described capture groove with described passing away.
Described capture groove by be sequentially communicated entrance area triangular in shape, rectangular capture region and triangular in shape
Exit region composition, described into passage and described entrance area position correspondence and to connect, multiple described enrichments are cheated
It is arranged in described capture region, described passing away and described exit region position correspondence and connects.In the structure,
Groove integrally hexagonal design is captured, from fluid-mechanics point of view, by program simulation trial, it was concluded that hexagonal
Body distribution is most uniform, and it can support the cell sorting of high flow capacity to capture.
Multiple described enrichment holes are arranged in array distribution in described capture region.It is provided with the outside of this chip
Magnetic field, when the circulating tumor cell of magnetic nano-particle mark flows through capture region, it will occur under the action of a magnetic force
Deflection perpendicular to flow velocity direction, enrichment hole is entered, so as to realize the sorting of circulating tumor cell, the setting in multiple enrichment holes
Improve the efficiency of sorting capture.
Described basic unit is silicon dioxide layer, and described microfluidic channel layer is silicon dioxide layer, plastic layer or resin
Layer, described signal amplification layer is silicon dioxide layer.In processing, by basic unit, microfluidic channel layer, signal amplification layer and nanometer
Layer gold is neatly superimposed successively, is put among 80 DEG C of baking oven and is solidified, and obtains complete structure.
The thickness of described basic unit is 1~2 millimeter, and the thickness of described microfluidic channel layer is 0.1~0.3 millimeter, institute
The thickness for the signal amplification layer stated is 10~30 microns.In the structure, each layer of thickness after Experimental comparison, reach with
Upper data, chip manufacturing cost is saved in the case where reaching efficient capture circulating tumor cell.
The surface modification of described capture groove has the anti-EpCAM antibody or anti-of specific recognition tumour cell
CD45 antibody.In the structure, anti-EpCAM antibody has the endogenic CTC cells of EpCAM antigens for capturing surface, and
CD45 is the general antigen of leucocyte, and the purpose using CD45 antibody is expressing the other thin of CD45 antigens in blood is removed
Born of the same parents, such as leucocyte, single ball etc. and the cell and albumen of the immune system of immunological regulation correlative, with reference to physics screening technique, with
Pure biology screening is compared, and is reduced cost of manufacture, is further reached the high purpose of separating purity.
Described basic unit is close to be arranged on described microfluidic channel layer, and the upper surface of described microfluidic channel layer is set
Capture groove is equipped with, the bottom land of described capture groove is provided with multiple enrichment holes, and the depth in described enrichment hole is 15~50 microns,
The upper surface of described basic unit is provided with signal amplification layer, on the upper surface of described signal amplification layer with described capture
Opening position is provided with nano gold layer corresponding to groove, through described nano gold layer, described signal amplification layer and described basic unit
It is provided with and enters passage and passing away, it is described to connect respectively with described capture groove with described passing away into passage
It is logical.For the ease of illustrating and taking into account the overall structure of micro-fluidic chip, the enrichment of this chip is designed with capturing CTC core position
In the centre of three-decker micro-fluidic chip, but it is in order at prevention CTC enrichments and the cross pollution being likely to occur during dye marker
With the convenience of practical application, microfluidic channel layer may be placed on bottom, is directly connected to by the micro-fluidic chip of commercialization
Sample to be enriched with enters passage and passing away, and basic unit, which is close to be arranged on described microfluidic channel layer, provides support, and
The position of signal amplification layer is constant.
Described capture groove by be sequentially communicated entrance area triangular in shape, rectangular capture region and triangular in shape
Exit region composition, described into passage and described entrance area position correspondence and to connect, multiple described enrichments are cheated
It is arranged in described capture region, described passing away and described exit region position correspondence and connects.In the structure,
Groove integrally hexagonal design is captured, from fluid-mechanics point of view, by program simulation trial, it was concluded that hexagonal
Body distribution is most uniform, and it can support the cell sorting of high flow capacity to capture.
Multiple described enrichment holes are arranged in array distribution in described capture region.It is provided with the outside of this chip
Magnetic field, when the circulating tumor cell of magnetic nano-particle mark flows through capture region, it will occur under the action of a magnetic force
Deflection perpendicular to flow velocity direction, enrichment hole is entered, so as to realize the sorting of circulating tumor cell, the setting in multiple enrichment holes
Improve the efficiency of sorting capture.
Compared with prior art, the utility model has the advantage of：By setting capture groove, capture in microfluidic channel layer
Groove is provided with multiple enrichment holes, wait the blood sorted after capture groove is entered into passage, in the presence of external magnetic field, and thing
First by magnetic nano-particle mark circulating tumor cell flow through enrichment hole when, will occur under the action of a magnetic force perpendicular to
The deflection in flow velocity direction, enrichment hole is entered, is avoided and is followed bad tumour cell and excessively assemble, and labeled cell is not then from row
Go out passage outflow, so as to realize the capture of circulating tumor cell；Signal amplification layer directly participate in capture cell before and after it is various
Process, by the signal enlarge-effect of top layer nanogold, amplification detection signal, follow-up quick analysis can be realized；This practicality
New Energy efficient capture circulating tumor cell, circulating tumor cell is not damaged, while facilitate subsequent detection.
Brief description of the drawings
Fig. 1 is the structural representation of the decomposing state of the utility model embodiment one, embodiment two and embodiment three；
Fig. 2 is the structural representation of the decomposing state of the utility model embodiment four.
Embodiment
Utility model is described in further detail below in conjunction with accompanying drawing embodiment.
Embodiment one：As illustrated, a kind of biochip for being used to screen rare cell in positioning and detection blood, including
Basic unit 1 and microfluidic channel layer 2, microfluidic channel layer 2 are close to be arranged in basic unit 1, and the upper surface of microfluidic channel layer 2 is set
There is capture groove 21, the bottom land of capture groove 21 is provided with multiple enrichment holes 22, and the depth in enrichment hole 22 is 15~50 microns, micro-fluidic
The upper surface of channel layer 2 is provided with signal amplification layer 3, on the upper surface of signal amplification layer 3 with 21 corresponding position of capture groove
The place of putting is provided with nano gold layer 4, is provided with into passage 5 and passing away 6, enters through nano gold layer 4 and signal amplification layer 3
Enter passage 5 and passing away 6 to connect with capture groove 21 respectively.
Embodiment two：As illustrated, a kind of biochip for being used to screen rare cell in positioning and detection blood, including
Basic unit 1 and microfluidic channel layer 2, microfluidic channel layer 2 are close to be arranged in basic unit 1, and the upper surface of microfluidic channel layer 2 is set
There is capture groove 21, the bottom land of capture groove 21 is provided with multiple enrichment holes 22, and the depth in enrichment hole 22 is 15~50 microns, micro-fluidic
The upper surface of channel layer 2 is provided with signal amplification layer 3, on the upper surface of signal amplification layer 3 with 21 corresponding position of capture groove
The place of putting is provided with nano gold layer 4, is provided with into passage 5 and passing away 6, enters through nano gold layer 4 and signal amplification layer 3
Enter passage 5 and passing away 6 to connect with capture groove 21 respectively.
In the present embodiment, capture groove 21 by be sequentially communicated entrance area 23 triangular in shape, rectangular capture region
24 and exit region 25 triangular in shape form, into passage 5 and the position correspondence of entrance area 23 and connect, multiple enrichment holes 22
It is arranged in capture region 24, passing away 6 and the position correspondence of exit region 25 and connects.
In the present embodiment, multiple enrichment holes 22 are arranged in capture region 24 in array distribution.The section shape in enrichment hole 22
Shape is triangle, rectangle, circle etc., is preferably circle, and the chi footpath scope in section is 10-20 microns.
When specifically used, magnetic nano-particle is put into blood sample first, magnetic nano-particle surface is with anti-
Body, by immune response absorption on target cell, then the blood sample that target cell has been labeled is entered from into passage 5
Enter among capture groove 21, in the presence of external magnetic field, the deflection perpendicular to flow velocity direction occurs for labeled CTC, enters
A labeled cell can only be accommodated by entering to enrichment hole 22, an enrichment hole 22, and labeled cell is not then from passing away 6
Outflow, takes out chip afterwards, injects a small amount of IR fluorescent dyes toward capture groove 21, with reference to nano gold layer 4, the detection letter that can amplify
Number intensity is better than traditional fluorescence signal, greatly facilitates subsequent analysis.
Embodiment three：As illustrated, a kind of biochip for being used to screen rare cell in positioning and detection blood, including
Basic unit 1 and microfluidic channel layer 2, microfluidic channel layer 2 are close to be arranged in basic unit 1, and the upper surface of microfluidic channel layer 2 is set
There is capture groove 21, the bottom land of capture groove 21 is provided with multiple enrichment holes 22, and the depth in enrichment hole 22 is 15~50 microns, micro-fluidic
The upper surface of channel layer 2 is provided with signal amplification layer 3, on the upper surface of signal amplification layer 3 with 21 corresponding position of capture groove
The place of putting is provided with nano gold layer 4, is provided with into passage 5 and passing away 6, enters through nano gold layer 4 and signal amplification layer 3
Enter passage 5 and passing away 6 to connect with capture groove 21 respectively.
In the present embodiment, capture groove 21 by be sequentially communicated entrance area 23 triangular in shape, rectangular capture region
24 and exit region 25 triangular in shape form, into passage 5 and the position correspondence of entrance area 23 and connect, multiple enrichment holes 22
It is arranged in capture region 24, passing away 6 and the position correspondence of exit region 25 and connects.
In the present embodiment, multiple enrichment holes 22 are arranged in capture region 24 in array distribution.The section shape in enrichment hole 22
Shape is triangle, rectangle, circle etc., preferably circle, and the chi footpath scope in section is 10~20 microns.
In the present embodiment, basic unit 1 is silicon dioxide layer, and microfluidic channel layer 2 is silicon dioxide layer, plastic layer or resin
Layer, signal amplification layer 3 is silicon dioxide layer.
In the present embodiment, the thickness of basic unit 1 is 1~2 millimeter, and the thickness of microfluidic channel layer 2 is 0.1~0.3 millimeter, letter
The thickness of number amplification layer 3 is 10~30 microns.
In the present embodiment, capture groove 21 surface modification have specific recognition tumour cell anti-EpCAM antibody or
Anti-CD45 antibody.The surface in particularly enrichment hole 22 is also equipped with anti-EpCAM antibody or anti-CD45 antibody.
Example IV：As illustrated, a kind of biochip for being used to screen rare cell in positioning and detection blood, including
Microfluidic channel layer 2 and basic unit 1, basic unit 1 are close to be arranged on microfluidic channel layer 2, and the upper surface of microfluidic channel layer 2 is set
There is capture groove 21, the bottom land of capture groove 21 is provided with multiple enrichment holes 22, and the depth in enrichment hole 22 is 15~50 microns, basic unit 1
Upper surface be provided with signal amplification layer 3, set on the upper surface of signal amplification layer 3 with capture 21 corresponding opening position of groove
There is nano gold layer 4, be provided with through nano gold layer 4, signal amplification layer 3 and basic unit 1 into passage 5 and passing away 6, into logical
Road 5 and passing away 6 connect with capture groove 21 respectively.
In the present embodiment, capture groove 21 by be sequentially communicated entrance area 23 triangular in shape, rectangular capture region
24 and exit region 25 triangular in shape form, into passage 5 and the position correspondence of entrance area 23 and connect, multiple enrichment holes 22
It is arranged in capture region 24, passing away 6 and the position correspondence of exit region 25 and connects.
In the present embodiment, multiple enrichment holes 22 are arranged in capture region 24 in array distribution.
Claims (9)
1. a kind of biochip for being used to screen rare cell in positioning and detection blood, including basic unit and microfluidic channel layer,
It is characterized in that：Described microfluidic channel layer is close to be arranged in described basic unit, the upper table of described microfluidic channel layer
Face is provided with capture groove, and the bottom land of described capture groove is provided with multiple enrichment holes, and the depth in described enrichment hole is 15~50
Micron, the upper surface of described microfluidic channel layer is provided with signal amplification layer, the upper surface of described signal amplification layer
Upper opening position corresponding with described capture groove is provided with nano gold layer, is put through described nano gold layer and described signal
Big layer is provided with into passage and passing away, it is described into passage and described passing away respectively with described capture groove
Connection.
2. a kind of biochip for being used to screen rare cell in positioning and detection blood according to claim 1, it is special
Sign is：Described capture groove by be sequentially communicated entrance area triangular in shape, rectangular capture region and triangular in shape
Exit region composition, described into passage and described entrance area position correspondence and to connect, multiple described enrichments are cheated
It is arranged in described capture region, described passing away and described exit region position correspondence and connects.
3. a kind of biochip for being used to screen rare cell in positioning and detection blood according to claim 2, it is special
Sign is：Multiple described enrichment holes are arranged in array distribution in described capture region.
4. a kind of biochip for being used to screen rare cell in positioning and detection blood according to claim 1, it is special
Sign is：Described basic unit is silicon dioxide layer, and described microfluidic channel layer is silicon dioxide layer, plastic layer or resin
Layer, described signal amplification layer is silicon dioxide layer.
5. a kind of biochip for being used to screen rare cell in positioning and detection blood according to claim 1, it is special
Sign is：The thickness of described basic unit is 1~2 millimeter, and the thickness of described microfluidic channel layer is 0.1~0.3 millimeter, described
Signal amplification layer thickness be 10~30 microns.
6. a kind of biochip for being used to screen rare cell in positioning and detection blood according to claim 1, it is special
Sign is：The surface modification of described capture groove has the anti-EpCAM antibody or anti-of specific recognition tumour cell
CD45 antibody.
7. a kind of biochip for being used to screen rare cell in positioning and detection blood, including microfluidic channel layer and basic unit,
It is characterized in that：Described basic unit is close to be arranged on described microfluidic channel layer, the upper table of described microfluidic channel layer
Face is provided with capture groove, and the bottom land of described capture groove is provided with multiple enrichment holes, and the depth in described enrichment hole is 15~50
Micron, the upper surface of described basic unit are provided with signal amplification layer, on the upper surface of described signal amplification layer with it is described
Capture groove corresponding to opening position be provided with nano gold layer, through described nano gold layer, described signal amplification layer and described
Basic unit be provided with into passage and passing away, it is described into passage and described passing away respectively with described capture
Groove connects.
8. a kind of biochip for being used to screen rare cell in positioning and detection blood according to claim 7, it is special
Sign is：Described capture groove by be sequentially communicated entrance area triangular in shape, rectangular capture region and triangular in shape
Exit region composition, described into passage and described entrance area position correspondence and to connect, multiple described enrichments are cheated
It is arranged in described capture region, described passing away and described exit region position correspondence and connects.
9. a kind of biochip for being used to screen rare cell in positioning and detection blood according to claim 8, it is special
Sign is：Multiple described enrichment holes are arranged in array distribution in described capture region.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720371980.5U CN206666547U (en) | 2017-04-11 | 2017-04-11 | A kind of biochip for being used to screen rare cell in positioning and detection blood |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720371980.5U CN206666547U (en) | 2017-04-11 | 2017-04-11 | A kind of biochip for being used to screen rare cell in positioning and detection blood |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206666547U true CN206666547U (en) | 2017-11-24 |
Family
ID=60380910
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720371980.5U Active CN206666547U (en) | 2017-04-11 | 2017-04-11 | A kind of biochip for being used to screen rare cell in positioning and detection blood |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206666547U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107058081A (en) * | 2017-04-11 | 2017-08-18 | 宁波美晶医疗技术有限公司 | A kind of biochip for being used to screen rare cell in positioning and detection blood |
| CN112191286A (en) * | 2020-09-29 | 2021-01-08 | 生物岛实验室 | Microfluidic chip and method for space omics sequencing and slide positioning identification |
| WO2022067567A1 (en) * | 2020-09-29 | 2022-04-07 | 生物岛实验室 | Microfluidic chip, and method for space omics sequencing and glass slide labeling |
-
2017
- 2017-04-11 CN CN201720371980.5U patent/CN206666547U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107058081A (en) * | 2017-04-11 | 2017-08-18 | 宁波美晶医疗技术有限公司 | A kind of biochip for being used to screen rare cell in positioning and detection blood |
| CN112191286A (en) * | 2020-09-29 | 2021-01-08 | 生物岛实验室 | Microfluidic chip and method for space omics sequencing and slide positioning identification |
| WO2022067567A1 (en) * | 2020-09-29 | 2022-04-07 | 生物岛实验室 | Microfluidic chip, and method for space omics sequencing and glass slide labeling |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107402295B (en) | Circulating tumor cell is automatically separated purifying micro-fluidic chip and its isolation and purification method | |
| CN106754240B (en) | For capturing and identifying the micro-fluidic chip of circulating tumor cell | |
| CN102925337B (en) | Microfluid cell capturing chip and manufacture method thereof | |
| CN105062866B (en) | For disposable separating chips module and the using method thereof of Peripheral Circulation tumour cell | |
| CN104651315B (en) | It is a kind of to recognize the method that tumour cell is sorted with cell size difference using antigen and antibody specific simultaneously in micro-fluidic chip | |
| CN206666547U (en) | A kind of biochip for being used to screen rare cell in positioning and detection blood | |
| CN103834558A (en) | Blood cell rapid sorting device and manufacturing method thereof | |
| CN108753571A (en) | A kind of cell typing capture chip and method | |
| CN103439241A (en) | Micro-fluidic chip detection system based on single-cell multi-parameter representation | |
| CN103266050A (en) | Microfluidic chip for sorting and application thereof | |
| CN208604119U (en) | Micro-fluidic chip for circulating tumor cell sorting | |
| Huang et al. | Rapid and precise tumor cell separation using the combination of size-dependent inertial and size-independent magnetic methods | |
| CN111744565B (en) | Microfluidic device and system for multi-channel parallel detection of cell deformability | |
| CN109486653A (en) | Trace cell capture system based on micro-fluidic and immune Magneto separate dual strategy | |
| Zeng et al. | Extraction of small extracellular vesicles by label-free and biocompatible on-chip magnetic separation | |
| CN109666584A (en) | A kind of experimental provision can be used for carrying out circulating tumor cell sorting experiment | |
| US9383355B2 (en) | Methods and related devices for continuous sensing utilizing magnetic beads | |
| CN112980677A (en) | Micro-fluidic chip for analyzing and sorting tumor cell migration capacity and preparation process | |
| CN107400623B (en) | Micro-fluidic chip for automatically capturing circulating tumor cells and automatic capturing method thereof | |
| CN108795693A (en) | A kind of micro-fluidic chip of capture blood rare cell | |
| CN103451087B (en) | Microfluidic chip capable of capturing tumor cells | |
| CN207130273U (en) | A kind of magnetic force micro-fluidic chip for capturing bacterium | |
| CN107148468A (en) | The microfluidic device with smooth surface for being enriched with rare cell and biomarker from biofluid | |
| CN207143246U (en) | A kind of integrated form Terahertz superstructure nano biological sensor | |
| Ni et al. | Inertia-magnetic microfluidics for rapid and high-purity separation of malignant tumor cells |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |