CN202164297U - Peripheral blood non-hematologic cell separating concentrating device - Google Patents
Peripheral blood non-hematologic cell separating concentrating device Download PDFInfo
- Publication number
- CN202164297U CN202164297U CN 201120283581 CN201120283581U CN202164297U CN 202164297 U CN202164297 U CN 202164297U CN 201120283581 CN201120283581 CN 201120283581 CN 201120283581 U CN201120283581 U CN 201120283581U CN 202164297 U CN202164297 U CN 202164297U
- Authority
- CN
- China
- Prior art keywords
- peripheral blood
- upper plate
- cell
- box body
- case body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
Abstract
The utility model belongs to the technical field of cell dissociation, and particularly relates to a peripheral blood non-hematologic cell separating concentrating device which comprises a hollow case body, wherein openings are formed at the top of the case body; an upper plate is fixedly arranged at the top of the case body in a jointing manner; openings corresponding to the openings at the top of the case body in position are formed on the upper plate; film substances corresponding to the openings at the top of the case body in position are arranged between the upper plate and the top of the case body; and a connecting pipe is arranged at the bottom of the case body. The peripheral blood non-hematologic cell separating concentrating device can achieve separation, concentration and verification of peripheral blood non-hematologic cells at the same time, achieves high efficiency for separation and verification, loses no cell, is simple and convenient to operate, and has the advantages of short reaction time, simplicity, effectiveness and practicability.
Description
Technical field
The utility model belongs to the cell separation technology field, is specifically related to the non-haematogenous cell separation and enrichment of a kind of peripheral blood device.
Background technology
The isolation identification of non-haematogenous cells such as maternal peripheral blood fetal cell and peripheral blood circulating tumor cell, cancer cells bolt has significant meaning to practice.The isolation identification of maternal peripheral blood fetal cell has important practical value for the diagnosis and the prevention of antenatal diagnosis, inborn defect.Separating and evaluation, the cultivation of cancer cells and the genetic analysis of cancer cells of peripheral blood circulating tumor cell (cancer cells) and cancer cells bolt (group), has significant application value for the transfer of tumour, the recurrence of tumour, the sensitivity testing of tumor chemotherapeutic drug, the prognosis and the early stage diagnosis of tumour.
At present, separate the peripheral blood tumour cell abroad and mainly contain three types, one type of magnetic bead that encapsulates with the relevant monoclonal antibody of cell is through the magnetic field separation cell; Though this method good separating effect; But technical sophistication, reagent be expensive, can only separate the tumour cell about 70%, and will carry out immunohistochemical methods and cytogenetic response in addition, can not in same device, react; Operation steps is many, and length expends time in; The another kind of film that utilizes is held back technology; Adopt the vacuum suction apparatus of robotization to separate the peripheral blood tumour cell; But can miss the less tumour cell of volume; And separation and evaluation work are proceed step by step, can not in same device, carry out isolation identification work, in transcellular process, possibly lose the tumour cell that has been separated to.The separation of maternal peripheral blood fetal cell, Shang Weijian has commercial device.The 3rd type is to utilize cellular segregation liquid to carry out density gradient centrifugation to obtain cancer cells, and wherein cancer cells is lost more.
The utility model content
The purpose of the utility model provides a kind of non-haematogenous cell separation and enrichment of peripheral blood device of realizing cellular segregation, enrichment, evaluation.
The utility model adopts following technical scheme:
The non-haematogenous cell separation and enrichment of a kind of peripheral blood device; Comprise a hollow box body; The top of box body is provided with perforate, and fitting on the top fixedly installs upper plate, and upper plate is provided with the perforate corresponding with the box body tip position; Position corresponding to perforate between upper plate and the box body top is provided with film property material, and the box body bottom is provided with pipe connecting.
The perforate of said upper plate is provided with sample intake passage, and sample intake passage is provided with interface.
Said film property material is fixed in upper plate bottom surface or box body top, perhaps is held between upper plate and the box body top.Be coated with antibody on the said film property material.
Said film property material is polycarbonate membrane or nitrocellulose filter.
When the utility model uses; The interface of sample intake passage is connected with the test tube appearance container of containing cell solution in the upper plate perforate; The pipe connecting of box body side bottom is connected with negative pressure device, carries out negative pressure leaching, and cell solution diafiltration in the test tube appearance container or perpendicular flow are through film property material; Need isolating target cell in the film property separating substances entrapped cell solution sample, realize the separation and concentration of cell; Can the cell of separation and concentration on film property material be placed on tissue culture flasks cultivates and treats further research and identify; Also can on film property material, encapsulate corresponding antibody in case of necessity; Specificity is caught corresponding target cell; Can be added to the reagent such as staining fluid of immunohistochemical methods then and carry out the immunohistochemical staining reaction on the film property material or carry out fluorescent dye; Thereby identify target cell, in same device, realize the separation and the evaluation of cell.The box body top and the upper plate of the utility model are provided with a plurality of perforates, can detect a plurality of samples simultaneously, and be directly external by the suction filtration delivery cassette through the waste liquid of film property material.
The utility model can be realized separation, enrichment and the evaluation of the non-haematogenous cell of peripheral blood simultaneously; The utility model also capable of using carries out cell cultures with the cell of enrichment, carries out that gene qualitative and quantitative analysis, immunohistochemical methods and FISH detect and experiments such as follow-up gene diagnosis and analysis, isolation identification efficient height; Do not lose cell; Easy and simple to handle, the reaction times is short, and is simple, effective, practical.
Description of drawings
Fig. 1 is the structural representation of hollow box body;
Fig. 2 is the structural representation of upper plate;
Fig. 3 is the structural representation of the utility model;
State graph when Fig. 4 uses for the utility model.
Embodiment
Like Fig. 1,2, shown in 3, the non-haematogenous cell separation and enrichment of peripheral blood device comprises hollow box body 1; The top of box body 1 is provided with perforate 2, and fitting on the top 1 fixedly installs upper plate 4, and upper plate 4 is provided with and the corresponding perforate 7 in box body 1 top drilling 2 positions; The perforate 7 of upper plate 4 is provided with sample intake passage 5; Sample intake passage 5 is provided with interface 6, and the position corresponding to perforate 2 between upper plate 4 and box body 1 top is provided with film property material 8, and the area of film property material 8 is greater than the area of perforate 2; Film property material 8 is held between upper plate 4 and box body 1 top, and box body 1 lateral bottom is provided with pipe connecting 3.
Film property material 8 is polycarbonate membrane or nitrocellulose filter, and those skilled in the art can select the aperture of film property material 8 according to the size of substantial sepn cell, can coated antibody on the film property material 8, and specificity is caught corresponding target cell.
As shown in Figure 4 during use; The interface 6 of upper plate 4 sample intake passages 5 is connected with the test tube appearance container of containing cell solution, and the pipe connecting 3 of box body 1 is connected with negative pressure device, carries out negative pressure leaching; Cell solution diafiltration in the test tube appearance container or perpendicular flow are through film property material 8; Film property material 8 separates in the entrapped cell solution sample and needs isolating target cell, realizes the separation and concentration of cell, is directly discharged outside the box body 1 by suction filtration through the waste liquid of film property material 8; Can be added to the reagent such as staining fluid of immunohistochemical methods then and carry out the immunohistochemical staining reaction on the film property material 8 or carry out fluorescent dye, thereby identify target cell, thereby realize the separation and the evaluation of target cell.
Claims (5)
1. the non-haematogenous cell separation and enrichment of peripheral blood device; It is characterized in that: comprise a hollow box body; The top of box body is provided with perforate, and fitting on the top fixedly installs upper plate, and upper plate is provided with and the corresponding perforate in box body top drilling position; Position corresponding to perforate between upper plate and the box body top is provided with film property material, and the box body side bottom is provided with pipe connecting.
2. the non-haematogenous cell separation and enrichment of peripheral blood as claimed in claim 1 device, it is characterized in that: the perforate of said upper plate is provided with sample intake passage, and sample intake passage is provided with interface.
3. according to claim 1 or claim 2 the non-haematogenous cell separation and enrichment of peripheral blood device, it is characterized in that: said film property material is fixed in upper plate bottom surface or box body top, perhaps is held between upper plate and the box body top.
4. the non-haematogenous cell separation and enrichment of peripheral blood as claimed in claim 3 device is characterized in that: be coated with antibody on the said film property material.
5. the non-haematogenous cell separation and enrichment of peripheral blood as claimed in claim 4 device, it is characterized in that: said film property material is polycarbonate membrane or nitrocellulose filter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120283581 CN202164297U (en) | 2011-08-05 | 2011-08-05 | Peripheral blood non-hematologic cell separating concentrating device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120283581 CN202164297U (en) | 2011-08-05 | 2011-08-05 | Peripheral blood non-hematologic cell separating concentrating device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202164297U true CN202164297U (en) | 2012-03-14 |
Family
ID=45800137
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120283581 Expired - Fee Related CN202164297U (en) | 2011-08-05 | 2011-08-05 | Peripheral blood non-hematologic cell separating concentrating device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202164297U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106459863A (en) * | 2014-05-01 | 2017-02-22 | 阿卜杜拉国王科技大学 | A microfluidic device that separates cells |
-
2011
- 2011-08-05 CN CN 201120283581 patent/CN202164297U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106459863A (en) * | 2014-05-01 | 2017-02-22 | 阿卜杜拉国王科技大学 | A microfluidic device that separates cells |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120314 Termination date: 20140805 |
|
| EXPY | Termination of patent right or utility model |