CN207845662U - A kind of anti-pollution lymphocyte culture bottle of anti-human CD3 optimizations - Google Patents

Abstract

The utility model discloses a kind of anti-pollution lymphocyte culture bottles of anti-human CD3 optimizations, including 3 layers of sieve being horizontally set in bottle, and the aperture of upper screen is 11 microns, the aperture of middle level sieve is 9 microns, the aperture of lower screen is 7 microns；Bottom of bottle is 0.2 0.3cm with the distance between sieve and two neighboring sieve.By adding the different sieve in aperture in Tissue Culture Flask, lymphocyte can be separately cultured in same system, advantageously reduce the growth that mutually squeezes and influence lymphocyte of the red blood cell between lymphocyte and lymphocyte, it is time saving and energy saving, and consumptive material can be saved, cost is reduced, while being conducive to the cell contamination problem for avoiding cumbersome operation from bringing.

Description

A kind of anti-pollution lymphocyte culture bottle of anti-human CD3 optimizations

Technical field

The utility model is related to field of cell culture more particularly to a kind of anti-pollution lymphocyte trainings of anti-human CD3 optimizations Support bottle.

Background technology

In anticoagulated whole blood, the size of red blood cell is 6~8.5 μm, is mostly 7 μm, lymphocyte can be divided into small by size Lymphocyte (5~8 μm of diameter), medium size lymphocyte (9~12 μm) and large lymphocyte (13~20 μm) are only deposited in normal blood In small lymphocyte and medium size lymphocyte, large lymphocyte sees the centrum germinativum of spleen and lymph node.In the prior art, different big Small lymphocyte and red blood cell mix culture, are also easy to produce mutually extruding and influence the growth of lymphocyte.

Under normal conditions, in order to be separately cultured, increase culture efficiency needs to centrifuge in cell liquid, red thin to remove Then born of the same parents carry out lymphocyte culture, not only complex steps, but also waste a large amount of consumptive materials, also increase the possibility of cell contamination Property.

Invention content

The purpose of this utility model is that in view of the deficiencies of the prior art, providing a kind of anti-pollution leaching of anti-human CD3 optimizations Bar Tissue Culture Flask.

The purpose of this utility model is achieved through the following technical solutions：A kind of anti-pollution lymph of anti-human CD3 optimizations The aperture of Tissue Culture Flask, including bottle and bottle cap, including 3 layers of sieve being horizontally set in bottle, upper screen is 11 micro- Rice, middle level sieve aperture be 9 microns, the aperture of lower screen is 7 microns；Between bottom of bottle and sieve and two neighboring sieve Distance is 0.2-0.3cm；The bottle cap top surface is provided with filter opening, and filter opening aperture is at 44A °~0.22 μm.

Further, the twine pan coating for constituting the sieve has anti-CD49d McAb layer.

Further, the bottle is connected through a screw thread and is formed by top, middle and upper part, middle and lower part, four part of lower part, described Top, middle and upper part, middle and lower part bottom surface be respectively upper screen, the middle level sieve that aperture is 9 microns, hole that aperture is 11 microns The lower screen that diameter is 7 microns, bottom face are bottle bottom surface.

The beneficial effects of the utility model are：The sieve different by adding aperture in Tissue Culture Flask, by lymph Cell is separately cultured in same system, and it is mutual between lymphocyte and lymphocyte to advantageously reduce red blood cell The growth of lymphocyte is squeezed and influences, it is time saving and energy saving, and consumptive material can be saved, cost is reduced, while being conducive to avoid numerous The cell contamination problem that trivial operation is brought.

Description of the drawings

Fig. 1 is the structural schematic diagram of the utility model；

Fig. 2 is the schematic diagram of the twine of sieve；

Fig. 3 is bottle cap schematic diagram；

In figure, top 1, middle and upper part 2, middle and lower part 3, lower part 4, upper screen 11, middle level sieve 12, lower screen 13, net Silk 21, anti-CD49d McAb layer 22；Bottle cap 5, filter opening 15.

Specific implementation mode

As shown in Figure 1, a kind of anti-pollution lymphocyte culture bottle of anti-human CD3 optimizations, including be horizontally set in bottle 3 layers of sieve, the aperture of upper screen 11 is 11 microns, the aperture of middle level sieve 12 is 9 microns, the aperture of lower screen 13 is 7 microns；The distance between two neighboring sieve is 1cm.Three-layer screen by bottle be divided into top 1, middle and upper part 2, middle and lower part 3, under 4 four part of portion；In use, cell liquid is added in cell bottle, the means such as wash away, rock by culture solution so that different big Small cell, which has respectively entered in different layers, to be separately cultured, and advantageously reduces red blood cell to lymphocyte and lymphocyte Between mutual squeeze and influence the growth of lymphocyte, while the cell contamination for being conducive to that cumbersome operation is avoided to bring is asked Topic.

As shown in figure 3, the bottle cap top surface is provided with filter opening 15,15 aperture of filter opening is at 44A °~0.22 μm, these filter opening energy The mechanically microorganisms such as filter bacteria, fungi prevent this quasi-microorganism from entering in culture bottle, and carbon dioxide, water vapour etc. Micro-molecular gas can then pass freely through, and into culture bottle, so in cell cultivation process, after tightening bottle cap, can both make Histocyte is grown under conditions of defined gas concentration lwevel and moisture content humidity, and can play prevents bacterium, fungi etc. micro- Biology into culture bottle and contamination.

As shown in Fig. 2, 21 pan coating of twine for constituting the sieve has anti-CD49d McAb layer 22.Utilize T lymphocyte tables Stimulant of the monoclonal antibody of face CD3 molecules as cell growth, can mass propgation lymphocyte, prepare it for chromosome With.

Preferably, the bottle is connected through a screw thread and is formed by top 1, middle and upper part 2, middle and lower part 3,4 four part of lower part, The top 1, middle and upper part 2, middle and lower part 3 bottom surface be respectively upper screen 11, aperture that aperture is 11 microns be in 9 microns Layer sieve 12, the lower screen 13 that aperture is 7 microns, bottom face are bottle bottom surface.After the completion of cell culture, top is first extracted 1 cell liquid after then detaching top, takes the cell liquid of middle and upper part 2, then takes the cell of middle and lower part 3, lower part 4 successively again Liquid.Or sieve is directly removed, carry out chromosome preparation.

Claims (3)

1. a kind of anti-pollution lymphocyte culture bottle of anti-human CD3 optimizations, including bottle and bottle cap, which is characterized in that including water Flat 3 layers of sieve being arranged in bottle, the aperture of upper screen (11) is 11 microns, the aperture of middle level sieve (12) is 9 microns, The aperture of lower screen (13) is 7 microns；Bottom of bottle is 0.2-0.3cm with the distance between sieve and two neighboring sieve；It is described Bottle cap (5) top surface is provided with filter opening (15), and filter opening aperture is at 44A °~0.22 μm.

2. culture bottle according to claim 1, which is characterized in that constitute the sieve twine (21) pan coating have it is anti- CD3 monoclonal antibodies layer (22).

3. culture bottle according to claim 1, which is characterized in that the bottle is by top (1), middle and upper part (2), middle and lower part (3), (4) four part of lower part is connected through a screw thread composition, the top (1), middle and upper part (2), middle and lower part (3) bottom surface be respectively Upper screen (11) that aperture is 11 microns, the middle level sieve (12) that aperture is 9 microns, the lower screen that aperture is 7 microns (13), bottom face is bottle bottom surface.