CN207619405U - A kind of cell cultivation instrument gas concentration lwevel control device - Google Patents

A kind of cell cultivation instrument gas concentration lwevel control device Download PDF

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Publication number
CN207619405U
CN207619405U CN201720826222.8U CN201720826222U CN207619405U CN 207619405 U CN207619405 U CN 207619405U CN 201720826222 U CN201720826222 U CN 201720826222U CN 207619405 U CN207619405 U CN 207619405U
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CN
China
Prior art keywords
circulating line
branch
gas concentration
concentration lwevel
branch circulating
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Expired - Fee Related
Application number
CN201720826222.8U
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Chinese (zh)
Inventor
王为民
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Tianjin Binhai New Area Ji Yu Automation Technology Co Ltd
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Tianjin Binhai New Area Ji Yu Automation Technology Co Ltd
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Priority to CN201720826222.8U priority Critical patent/CN207619405U/en
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Publication of CN207619405U publication Critical patent/CN207619405U/en
Expired - Fee Related legal-status Critical Current
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Abstract

The utility model provides a kind of cell cultivation instrument gas concentration lwevel control device, including:Culturing room, feeder and concentration control apparatus;The feeder includes carbon dioxide air source and is set to the indoor device for generating air curtain of culture, and the carbon dioxide air source is connect with the device for generating air curtain by steam line;The device for generating air curtain includes circulating line and multiple outlet nozzles for being connect with the circulating line;The concentration control apparatus includes the reset valve, pressure sensor, miniature electromagnetic valve being set on the steam line, the gas concentration lwevel sensor being set to inside the culturing room, and the control unit that is connect respectively with the reset valve, pressure sensor, miniature electromagnetic valve, gas concentration lwevel sensor.

Description

A kind of cell cultivation instrument gas concentration lwevel control device
Technical field
The utility model is related to technical field of cell culture, and in particular to a kind of cell cultivation instrument gas concentration lwevel control Device.
Background technology
Cell culture invitro is that tissue or living cells living, the liquid being inoculated in specific culture vessel are taken out out of organism In state culture medium, wherein containing in a organized way or multiple nutritional components necessary to cell growth breeding, and it is special to place it in simulation Determine in organisms in the incubator of living environment, to be allowed to grow, be proliferated.
Animal cell culture needs to be continuing to supply oxygen and excludes carbon dioxide, and culture plant cell in contrast, is cultivated When different cell, the requirement for cultivating the gas concentration lwevel in instrument in air is different, and can be matched and specifically be contained according to demand There is the mixed gas of gas concentration lwevel, when gas concentration lwevel needed for experiment is different every time, it is also necessary to match different dioxies The mixed gas for changing concentration of carbon, makes troubles to operation.
Utility model content
In view of this, the utility model provides a kind of cell cultivation instrument gas concentration lwevel control device, can control automatically The concentration of carbon dioxide, adapts it to the demand of different gas concentration lwevels in system culture instrument.
The utility model provides a kind of cell cultivation instrument gas concentration lwevel control device, it is characterised in that:The cell Cultivating instrument gas concentration lwevel control device includes:Culturing room, feeder and concentration control apparatus;
The feeder includes carbon dioxide air source and is set to the indoor device for generating air curtain of culture, described Carbon dioxide air source is connect with the device for generating air curtain by steam line;
The device for generating air curtain includes circulating line and multiple outlet nozzles for being connect with the circulating line;
The concentration control apparatus includes the reset valve, pressure sensor, micro electromagnetic being set on the steam line Valve, the gas concentration lwevel sensor being set to inside the culturing room, and respectively with the reset valve, pressure sensor, The control unit that miniature electromagnetic valve, gas concentration lwevel sensor connect;
The gas concentration lwevel sensor is used to detect the concentration of the culture indoor carbon dioxide, and by testing result It is transferred to described control unit, described control unit controls the confession according to the testing result of the gas concentration lwevel sensor Device of air supplies for the culturing room.
Preferably, the circulating line of the device for generating air curtain includes major cycle pipeline and branch circulating line, described It is connected between major cycle pipeline and the branch circulating line;
The branch circulating line includes first branch circulating line and the second branch circulating line, and the first branch is followed Endless tube road and the second branch circulating line are circular pipeline, and the first branch circulating line and described second Branch circulating line is arranged concentrically, and the diameter of the second branch circulating line is more than the straight of the first branch circulating line Diameter;
The major cycle pipeline is distributed along a diameter of the second branch air supply pipe, is connected to the first branch cycle The center of pipeline and the second branch circulating line, the major cycle pipeline is provided with air inlet, the main circulation pipe Road is connected to by the air inlet with the steam line;
The major cycle pipeline is respectively arranged with first branch circulating line control valve and the control of the second branch circulating line Valve, the first branch circulating line control valve and the second branch circulating line control valve are connect with described control unit.
Preferably, multiple outlet nozzles are distributed on the first branch circulating line, the outlet nozzle is uniformly distributed In on circular first branch circulating line;
Multiple outlet nozzles are distributed on the second branch circulating line, the outlet nozzle is uniformly distributed in annular shape The second branch circulating line on;
The outlet nozzle number being distributed on the first branch circulating line and the second branch circulating line is equal, and It is interspersed.
Preferably, the outlet nozzle is shower shape outlet nozzle, is evenly arranged in the shower shape outlet nozzle more A venthole.
Preferably, the surface of the shower shape outlet nozzle is convex spherical.
Preferably, the cell cultivation instrument gas concentration lwevel control device is additionally provided with human-computer interaction interface, the people Machine interactive interface is connect with described control unit.
The utility model has the advantages and positive effects of:The utility model is provided with gas concentration lwevel control dress It sets, user can be arranged as required to different carbon dioxide concentration parameters, and can detect culture indoor two in real time Concentration of carbon is aoxidized, and transport of carbon dioxide in culturing room is want to control feeder according to real-time gas concentration lwevel.
The utility model is provided with device for generating air curtain simultaneously, passes through the device for generating air curtain, carbon dioxide gas physical efficiency It is enough to be uniformly invested in the culturing room so that the measurement of the concentration of carbon dioxide is more accurate.
Description of the drawings
Fig. 1 is the structural schematic diagram of the cell cultivation instrument gas concentration lwevel control device of the utility model;
Fig. 2 is the structural schematic diagram of the device for generating air curtain of the utility model;
Fig. 3 is the structural schematic diagram of the outlet nozzle of the utility model.
Specific implementation mode
In order to be better understood from the utility model, with reference to specific embodiments and the drawings to the utility model into advance one The description of step.
The utility model provides a kind of cell cultivation instrument gas concentration lwevel control device, the cell cultivation instrument titanium dioxide Concentration of carbon control device includes:Culturing room 1, feeder and concentration control apparatus.The feeder is used for the training Transport of carbon dioxide in room 1 is supported, the concentration control apparatus is used to detect the gas concentration lwevel in the culturing room 1, and root It is 1 transport of carbon dioxide of the culturing room to control the feeder according to the gas concentration lwevel in the culturing room 1.
Further, as shown in Figure 1, the feeder includes carbon dioxide air source 2 and is set to the culturing room Interior device for generating air curtain 3, the carbon dioxide air source 2 are connect with the device for generating air curtain 3 by steam line 4.It is described Carbon dioxide gas is delivered to the device for generating air curtain 3, the air curtain by carbon dioxide air source 2 by the steam line 4 Carbon dioxide gas is delivered in the form of air curtain in the culturing room 1 by generating means 3.
Further, the concentration control apparatus includes reset valve 41, the pressure sensing being set on the steam line 4 Device 42, miniature electromagnetic valve 43, the gas concentration lwevel sensor 5 being set to inside the culturing room 1, and respectively with it is described micro- The control unit 6 for adjusting valve 41, pressure sensor 42, miniature electromagnetic valve 43, gas concentration lwevel sensor 5 to connect.
The pressure sensor 42 is used to measure the pressure value of the carbon dioxide air source 2, and the pressure measured Value is transferred to described control unit 6, and when the pressure value in carbon dioxide air source 2 is higher than preset pressure value, the control is single Member 6 controls to adjust the reset valve 41, the supply gas pressure of the carbon dioxide air source 2 is reduced, when in the carbon dioxide air source 2 Pressure value be less than preset pressure value when, described control unit 6 controls to adjust the reset valve 41, increases the carbon dioxide The supply gas pressure of air source 2, the preset pressure value can be adjusted according to specific needs.
The gas concentration lwevel sensor 5 is used to measure the gas concentration lwevel in the culturing room 1, and will measure To carbon dioxide concentration value be transferred to described control unit 6, described control unit 6 by the carbon dioxide concentration value received with Preset carbon dioxide concentration value is compared, when the gas concentration lwevel in the culturing room 1 is less than preset carbon dioxide When concentration, starts the feeder and supplied for the culturing room 1.
Specifically, the gas concentration lwevel sensor 5 is connect by D/A converting circuit with described control unit 6, institute The analog quantity for stating the perception of gas concentration lwevel sensor 5 is converted into digital quantity by D/A converting circuit, and described control unit 6 connects The data by D/A converting circuit conversion are received, are compared with preset data, described control unit 6 is transported by PID It calculates and controls the time that the miniature electromagnetic valve 43 opens and closes.
Further, the utility model is additionally provided with human-computer interaction interface 7, the human-computer interaction interface 7 and the control Unit 6 connects, and by the human-computer interaction interface 7, preset pressure value and preset carbon dioxide concentration value can be arranged, together When can show carbon dioxide sensor 5 measure real-time carbon dioxide concentration value and the pressure sensor measure reality When pressure value, can also show the reset valve, miniature electromagnetic valve on off state.
Further, the utility model is additionally provided with warning device 8, and the warning device 8 connects with described control unit 6 It connects, when the real-time pressure value that the real-time carbon dioxide concentration value or the pressure sensor that carbon dioxide sensor measures measure When more than preset carbon dioxide concentration value or preset pressure value, the warning device 8 can send out alarm, remind and use Person.
Further, the device for generating air curtain 3 includes circulating line 31 and is connect with the circulating line multiple Outlet nozzle 32.
Further, the circulating line 31 includes major cycle pipeline 311 and branch circulating line 312, and the master follows It is connected between endless tube road 311 and the branch circulating line 312;The branch circulating line 312 includes first branch circulation pipe Road 3121 and the second branch circulating line 3122, the first branch circulating line 3121 and the second branch circulating line 3122 be circular pipeline, and the first branch circulating line 3121 and the second branch circulating line 3122 are concentric Setting, the diameter of the second branch circulating line 3122 are more than the diameter of the first branch circulating line 3121;The master Circulating line 311 is distributed along a diameter of the second branch circulating line 3122, is connected to the first branch circulating line 3121 and the second branch circulating line 3122, the center of the major cycle pipeline 311 be provided with air inlet 3111, institute Major cycle pipeline 311 is stated to be connected to the steam line 4 by the air inlet 3111.
The major cycle pipeline 311 is respectively arranged with first branch circulating line control valve 3112 and the second branch circulation pipe Road control valve 3113, the first branch circulating line control valve 3112 and the second branch circulating line control valve 3113 with Described control unit 6 connects.
When gas concentration lwevel that the gas concentration lwevel sensor 5 perceives with preset gas concentration lwevel very When close, described control unit 6, which can control, closes the second branch circulating line control valve 3113 so that carbon dioxide gas Body only recycles in the first branch circulating line 3121.
Further, multiple outlet nozzles 32, the outlet nozzle is distributed on the first branch circulating line 3121 32 are uniformly distributed on circular first branch circulating line 3121;It is distributed on the second branch circulating line 3122 more A outlet nozzle 32, the outlet nozzle 32 are uniformly distributed on circular the second branch circulating line 3122;Described first 32 numbers of outlet nozzle being distributed on branch circulating line 3121 and the second branch circulating line 3122 are equal, and staggeredly divide Cloth.
Further, the outlet nozzle 32 is shower shape outlet nozzle, is uniformly arranged in the shower shape outlet nozzle There are multiple ventholes 321.The surface of the shower shape outlet nozzle 32 is convex spherical.
It should be noted that herein, relational terms such as first and second and the like are used merely to a reality Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation In any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended to Non-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including those Element, but also include other elements that are not explicitly listed, or further include for this process, method, article or equipment Intrinsic element.In the absence of more restrictions, the element limited by sentence "including a ...", it is not excluded that There is also other identical elements in process, method, article or equipment including the element.
Finally it should be noted that:Obviously, above-described embodiment is only intended to clearly illustrate the utility model example, And it does not limit the embodiments.For those of ordinary skill in the art, may be used also on the basis of the above description To make other variations or changes in different ways.There is no necessity and possibility to exhaust all the enbodiments.And thus Among changes and variations that derived from are still in the scope of protection of the utility model.

Claims (6)

1. a kind of cell cultivation instrument gas concentration lwevel control device, it is characterised in that:The cell cultivation instrument carbon dioxide is dense Spending control device includes:Culturing room, feeder and concentration control apparatus;
The feeder includes carbon dioxide air source and is set to the indoor device for generating air curtain of the culture, the dioxy Change carbon air source to connect by steam line with the device for generating air curtain;
The device for generating air curtain includes circulating line and multiple outlet nozzles for being connect with the circulating line;
The concentration control apparatus includes the reset valve, pressure sensor, miniature electromagnetic valve being set on the steam line, if The gas concentration lwevel sensor being placed in inside the culturing room, and respectively with the reset valve, pressure sensor, micro electric The control unit that magnet valve, gas concentration lwevel sensor connect;
The gas concentration lwevel sensor is used to detect the concentration of the culture indoor carbon dioxide, and testing result is transmitted To described control unit, described control unit controls the gas supply according to the testing result of the gas concentration lwevel sensor and fills It is set to culturing room's gas supply.
2. cell cultivation instrument gas concentration lwevel control device according to claim 1, it is characterised in that:The air curtain hair The circulating line of generating apparatus includes major cycle pipeline and branch circulating line, the major cycle pipeline and the branch circulation pipe It is connected between road;
The branch circulating line includes first branch circulating line and the second branch circulating line, the first branch circulation pipe Road and the second branch circulating line are circular pipeline, and the first branch circulating line and the second branch Circulating line is arranged concentrically, and the diameter of the second branch circulating line is more than the diameter of the first branch circulating line;
The major cycle pipeline is distributed along a diameter of the second branch air supply pipe, is connected to the first branch circulating line The center of the second branch circulating line, the major cycle pipeline is provided with air inlet, the major cycle pipeline is logical The air inlet is crossed to be connected to the steam line;
The major cycle pipeline is respectively arranged with first branch circulating line control valve and the second branch circulating line control valve, institute It states first branch circulating line control valve and the second branch circulating line control valve is connect with described control unit.
3. cell cultivation instrument gas concentration lwevel control device according to claim 2, it is characterised in that:Described first Multiple outlet nozzles are distributed on the circulating line of road, the outlet nozzle is uniformly distributed in circular first branch circulating line On;
It is distributed with multiple outlet nozzles on the second branch circulating line, the outlet nozzle is uniformly distributed in circular the On two branch circulating lines;
The outlet nozzle number being distributed on the first branch circulating line and the second branch circulating line is equal, and staggeredly Distribution.
4. cell cultivation instrument gas concentration lwevel control device according to claim 1 or 3, it is characterised in that:It is described go out Gas nozzle is shower shape outlet nozzle, and multiple ventholes are evenly arranged in the shower shape outlet nozzle.
5. cell cultivation instrument gas concentration lwevel control device according to claim 4, it is characterised in that:The shower shape The surface of outlet nozzle is convex spherical.
6. cell cultivation instrument gas concentration lwevel control device according to claim 1, it is characterised in that:The cell training It supports instrument gas concentration lwevel control device and is additionally provided with human-computer interaction interface, the human-computer interaction interface connects with described control unit It connects.
CN201720826222.8U 2017-07-07 2017-07-07 A kind of cell cultivation instrument gas concentration lwevel control device Expired - Fee Related CN207619405U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201720826222.8U CN207619405U (en) 2017-07-07 2017-07-07 A kind of cell cultivation instrument gas concentration lwevel control device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201720826222.8U CN207619405U (en) 2017-07-07 2017-07-07 A kind of cell cultivation instrument gas concentration lwevel control device

Publications (1)

Publication Number Publication Date
CN207619405U true CN207619405U (en) 2018-07-17

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115627214A (en) * 2022-10-28 2023-01-20 中国科学院苏州生物医学工程技术研究所 Three-gas multi-channel gas supply system and method for incubator

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115627214A (en) * 2022-10-28 2023-01-20 中国科学院苏州生物医学工程技术研究所 Three-gas multi-channel gas supply system and method for incubator

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CF01 Termination of patent right due to non-payment of annual fee
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Granted publication date: 20180717

Termination date: 20210707