CN111685051B - Animal hypoxia/reoxygenation culture device - Google Patents
Animal hypoxia/reoxygenation culture device Download PDFInfo
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- CN111685051B CN111685051B CN202010623099.6A CN202010623099A CN111685051B CN 111685051 B CN111685051 B CN 111685051B CN 202010623099 A CN202010623099 A CN 202010623099A CN 111685051 B CN111685051 B CN 111685051B
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- 241001465754 Metazoa Species 0.000 title claims abstract description 71
- 206010021143 Hypoxia Diseases 0.000 title claims abstract description 47
- 230000007954 hypoxia Effects 0.000 title claims abstract description 47
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 80
- 239000001301 oxygen Substances 0.000 claims abstract description 80
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 80
- 238000001514 detection method Methods 0.000 claims abstract description 10
- 238000005086 pumping Methods 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 235000013305 food Nutrition 0.000 claims description 15
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 238000010030 laminating Methods 0.000 claims description 5
- 239000000741 silica gel Substances 0.000 claims description 5
- 229910002027 silica gel Inorganic materials 0.000 claims description 5
- 238000012544 monitoring process Methods 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 238000005192 partition Methods 0.000 abstract description 9
- 238000010339 medical test Methods 0.000 abstract description 2
- 238000000605 extraction Methods 0.000 abstract 1
- 230000009286 beneficial effect Effects 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 238000010171 animal model Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 230000007774 longterm Effects 0.000 description 4
- 230000001276 controlling effect Effects 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 208000037273 Pathologic Processes Diseases 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 244000144993 groups of animals Species 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000009054 pathological process Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/03—Housing for domestic or laboratory animals
- A01K1/031—Cages for laboratory animals; Cages for measuring metabolism of animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/035—Devices for use in keeping domestic animals, e.g. fittings in housings or dog beds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/035—Devices for use in keeping domestic animals, e.g. fittings in housings or dog beds
- A01K1/0356—Feeding or drinking devices associated with cages
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- General Health & Medical Sciences (AREA)
- Birds (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to the field of medical test equipment, and relates to an animal hypoxia/reoxygenation culture device, wherein an incubator is divided into a plurality of culture cavities by a partition board, each culture cavity is respectively provided with a culture extraction box which is independently opened and closed, the upper surface of the incubator is provided with a transparent cover plate, and a feeding kettle is arranged on the transparent cover plate; the connection controller comprises an oxygen concentration detection conduit and a branch air inlet conduit which are communicated with each culture suction box and the control box, each branch air inlet conduit is communicated with the main air inlet conduit through a three-way connecting pipe, an air inlet control valve is arranged on each branch air inlet conduit, and the air inlet control valve is in signal connection with the control box through a wire with a wire conduit; the control box is provided with an oxygen concentration sensor and an oxygen concentration controller, and the animal hypoxia/reoxygenation culture device can simultaneously meet the requirements of the independence, automatic control, long-time culture and hypoxia/reoxygenation of animal culture.
Description
Technical Field
The invention belongs to the field of medical test equipment, relates to an animal hypoxia/reoxygenation culture device, and particularly relates to an animal hypoxia/reoxygenation culture device which can effectively regulate the oxygen concentration in an incubator, create a hypoxia/reoxygenation culture environment required by an animal model and ensure that the culture environment of a control group is not interfered to the maximum extent.
Background
The change in oxygen content is one of the most important regulators throughout the life cycle, extending from embryonic development, maintenance of normal body function, to pathological processes such as disease and aging, where hypoxia is one of the most common basic environments. Hypoxia culture is a culture state when oxygen in cells or organs is lower than the oxygen level in air, and has an important role in human medical research. In the prior art, an experimental animal model needs to be established by simulating a hypoxia environment, and the experimental animal model is used for researching a series of pathological, physiological and molecular biological index changes of animals or cells under the relatively hypoxia environment. The conventional hypoxia incubator is a hypoxia state formed by premixing air and nitrogen in a certain proportion, and is generally simple in design, and generally has the following problems:
1. traditional animal incubator can only realize the creation of hypoxia environment, and the function is single, and traditional animal culture experiment not only can use hypoxia culture environment, can use reoxygenation environment in more time moreover, because traditional experimental apparatus's function is single, when constructing reoxygenation environment, often oxygen concentration resumes the speed slowly, and long-time recovery process can influence experimental model's precision moreover.
2. The traditional animal incubator has the defect of concentrated culture, the control group is cultured together with animals of the experimental group, the relatively independent culture environment is lacking, some animals need to be treated in the culture process, but the animals are cultured together, when treated, the culture environment of other groups is inevitably influenced, the construction process of an animal model is not strict, and the experimental result is finally influenced.
3. Traditional animal incubator lacks relative independence and long-term system of raising, when needs put in food and water for the animal, need open the incubator, put in a certain amount of water and food to the incubator in, can inevitably bring the influence to the culture environment after opening the incubator, need readjust oxygen concentration, not only waste time and energy, and process time is long moreover, is difficult to guarantee whole culture process, animal culture environment's continuity and stability, finally influences experimental result.
4. The traditional animal incubator lacks automatic closed-loop control, and many times the regulation of oxygen concentration need human manual intervention to long-time regulation and control oxygen's inflow repeatedly, whole adjustment process is loaded down with trivial details and the degree of accuracy is low, causes experimental efficiency low, delays the experiment progress, extravagant experiment air supply.
Disclosure of Invention
In view of this, the invention provides an animal hypoxia/reoxygenation culture device which can simultaneously satisfy the realization of the independence, automatic control, long-time culture and hypoxia/reoxygenation of animal culture in order to solve the problems of single function, inconvenience in accurate automatic treatment and control of each animal and influence on the accuracy of experimental results of the traditional animal incubator.
In order to achieve the above purpose, the invention provides an animal hypoxia/reoxygenation culture device, comprising an incubator, a control box and a connection controller;
Wherein two or more than two partition boards which are the same as the end face of the incubator are arranged in the incubator and are separated into a plurality of culture cavities by the partition boards, each culture cavity is respectively provided with a culture box which is independently opened and closed, the upper surface of the incubator is provided with a transparent cover plate, and a feeding kettle for supplying nutrients for animals in the culture boxes is arranged on the transparent cover plate;
The connection controller comprises an oxygen concentration detection conduit and a branch air inlet conduit which are communicated with each culture suction box and the control box, each branch air inlet conduit is communicated with the main air inlet conduit through a three-way connecting pipe, an air inlet control valve is arranged on each branch air inlet conduit, and the air inlet control valve is in signal connection with the control box through a wire with a wire conduit;
An oxygen concentration sensor for monitoring the oxygen concentration in each culture pumping box in real time and an oxygen concentration controller for controlling the opening of an air inlet control valve on the branch air inlet pipe are arranged on the control box.
The beneficial effect of this basic scheme lies in: each culture cavity in the incubator, which is evenly separated by the partition plate, is used for respectively culturing single-group animals, provides the same living place for the animals, and does not affect the culture environment of other animals when the single animals are treated. The drawing type cultivation drawing box is convenient for taking animals. The transparent cover plate on the incubator is convenient for an experiment operator to observe and record the culture process of animals, and the feeding kettle on the transparent cover plate is used for providing necessary water and nutrient substances for the animals in the culture box, so that the long-term culture of the animals is realized. The oxygen concentration detection conduit is used for realizing real-time adjustment of the oxygen concentration in each incubator. Each culture pumping box is communicated with the main air inlet pipe through a three-way connecting pipe on the branch air inlet pipe, and different mediums (O 2 or N 2) are introduced into the main air inlet pipe, so that the switching of the hypoxia/reoxygenation culture conditions in each culture pumping box is realized. The air inlet control valve on each branch air inlet pipe is in signal connection with the oxygen concentration controller on the control box, the incubator is communicated with the control box through the oxygen concentration detection catheter to form a closed loop, the oxygen concentration in each culture pumping box is monitored in real time through the oxygen concentration sensor on the control box, the oxygen concentration sensor sends a control signal to the oxygen concentration controller according to the set oxygen concentration requirement, then the opening of the air inlet control valve on each branch air inlet pipe is controlled, the amount of gas entering each culture pumping box is adjusted, and accurate automatic control of the oxygen concentration in each culture pumping box is realized.
Further, the first pipe connecting hole that link up has been seted up to the casing of transparent apron, incubator and cultivate and take out the box upper surface, and oxygen concentration detects pipe one end and passes first pipe connecting hole and every cultivation and take out the box intercommunication, and the second pipe connecting hole has been seted up to the casing left side lateral wall lower part of incubator, cultivates to take out to be provided with on the box and take out the box inlet port of taking out with the cultivation that the second pipe connecting hole link up, and branch air inlet pipe one end passes the second pipe connecting hole and takes out the box intercommunication with every cultivation, and the exhaust hole that link up has been seted up on the casing of incubator and the right side lateral wall upper portion of taking out the box with cultivateing.
Further, an oxygen concentration display screen for displaying the oxygen concentration in the control box is arranged on the control box. The beneficial effects are that: the oxygen concentration display screen is used for displaying the oxygen concentration in the control box, and the oxygen concentration controller can be set and controlled.
Further, the contact surface of the culture drawer and the incubator is provided with a silica gel laminating layer. The beneficial effects are that: the silica gel laminating layer of the contact surface of the culture drawer box and the incubator can ensure that the culture drawer box has tightness after being closed, and prevents air leakage or animal escape.
Further, the end face of the incubator is in a right-angle trapezoid shape. The beneficial effects are that: the end face of the incubator is in a right-angle trapezoid shape, and the transparent cover plate on the upper surface of the incubator is in a slope shape, so that water and nutrition can be conveniently supplied to the water kettle for animals in each cultivating drawer.
Further, a food placing area for placing food is arranged in each cultivation box. The beneficial effects are that: the food placing area in the culture drawer box is used for placing food for animal survival.
Further, the transparent cover plate and the top of the culture drawing box are provided with through small holes, and the water outlet end of the water feeding kettle is fixedly provided with a water delivery pipe with a free end penetrating through the small holes.
Further, a one-way exhaust valve is fixedly arranged on the exhaust hole. The beneficial effects are that: the exhaust hole with the one-way exhaust valve is convenient for the discharge of malodor generated by animals in the culture pumping box.
The invention has the beneficial effects that:
1. According to the animal hypoxia/reoxygenation culture device disclosed by the invention, the incubator is divided into a plurality of culture cavities through the partition plates, so that different groups of animals can be cultured in independent spaces, each group of experimental animals can be conveniently treated at any time, the animal culture environments of other groups are not influenced during treatment, and independent culture is realized. In the aspect of oxygen concentration control, an oxygen concentration sensor sends a control signal to an oxygen concentration controller according to a set oxygen concentration requirement, so that the opening of an air inlet control valve on each branch air inlet pipe is controlled, the amount of air entering each culture pumping box is regulated, and the accurate automatic control of the oxygen concentration in each culture pumping box is realized. By introducing different media (O 2 or N 2) into the main air inlet pipe, the switching of the hypoxia/reoxygenation culture conditions in each culture pumping box is realized rapidly. In addition, a feeding system is designed for ensuring continuous cultivation for a relatively long time, long-term cultivation can be realized under the condition that the incubator is not opened, and the cultivation environment of other incubators is not influenced even if the incubator is opened.
2. Compared with the traditional manual-adjustment hypoxia incubator, the animal hypoxia/reoxygenation culture device disclosed by the invention realizes accurate closed-loop automatic control of the oxygen concentration in the incubator, and greatly improves the adjustment efficiency of the oxygen concentration. According to the different introduced gas mediums, the two functions of rapid hypoxia/reoxygenation can be realized in the same device. The independent culture box is adopted, so that animals in the box can be conveniently treated at any time, and animal culture in other boxes is not disturbed. The kettle and the food placement area arranged by the device ensure the long-term continuity of animal culture to the maximum extent, and avoid the interference of experimental conditions caused by the necessary food throwing. The animal hypoxia/reoxygenation culture device can simultaneously meet the requirements of independence, automatic control, long-time culture and realization of hypoxia/reoxygenation of animal culture.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention. The objects and other advantages of the invention may be realized and obtained by means of the instrumentalities and combinations particularly pointed out in the specification.
Drawings
For the purpose of making the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in the following preferred detail with reference to the accompanying drawings, in which:
FIG. 1 is a schematic diagram of the structure of an animal hypoxia/reoxygenation culture device according to the present invention;
FIG. 2 is a schematic diagram showing the structure of an incubator in the animal hypoxia/reoxygenation culture apparatus of the present invention;
FIG. 3 is a schematic view showing the structure of a partition plate installed in an incubator in the animal hypoxia/reoxygenation culture apparatus of the present invention;
FIG. 4 is a schematic diagram of the structure of a culture drawer in the animal hypoxia/reoxygenation culture device of the present invention;
FIG. 5 is a schematic view showing the structure of a transparent cover plate in the animal hypoxia/reoxygenation culture device of the present invention;
FIG. 6 is a schematic diagram showing the structure of a partition plate in the animal hypoxia/reoxygenation culture apparatus of the present invention;
FIG. 7 is a schematic diagram showing the structure of the shell of the incubator in the animal hypoxia/reoxygenation culture apparatus of the present invention.
Reference numerals: oxygen concentration controller 1, control box 2, oxygen concentration sensor 3, oxygen concentration display screen 4, oxygen concentration detection pipe 5, feeding kettle 6, first pipe connecting hole 7, incubator 8, second pipe connecting hole 9, air intake control valve 10, branch air intake pipe 11, main air intake pipe 12, tee joint pipe 13, wire 14, wire pipe 15, exhaust hole 16, cultivation taking out box 17, baffle 18, transparent cover plate 19, cultivation taking out box inlet port 20, silica gel laminating layer 21, food placement area 22.
Detailed Description
Other advantages and effects of the present invention will become apparent to those skilled in the art from the following disclosure, which describes the embodiments of the present invention with reference to specific examples. The invention may be practiced or carried out in other embodiments that depart from the specific details, and the details of the present description may be modified or varied from the spirit and scope of the present invention. It should be noted that the illustrations provided in the following embodiments merely illustrate the basic idea of the present invention by way of illustration, and the following embodiments and features in the embodiments may be combined with each other without conflict.
Wherein the drawings are for illustrative purposes only and are shown in schematic, non-physical, and not intended to limit the invention; for the purpose of better illustrating embodiments of the invention, certain elements of the drawings may be omitted, enlarged or reduced and do not represent the size of the actual product; it will be appreciated by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.
The same or similar reference numbers in the drawings of embodiments of the invention correspond to the same or similar components; in the description of the present invention, it should be understood that, if there are terms such as "upper", "lower", "left", "right", "front", "rear", etc., that indicate an azimuth or a positional relationship based on the azimuth or the positional relationship shown in the drawings, it is only for convenience of describing the present invention and simplifying the description, but not for indicating or suggesting that the referred device or element must have a specific azimuth, be constructed and operated in a specific azimuth, so that the terms describing the positional relationship in the drawings are merely for exemplary illustration and should not be construed as limiting the present invention, and that the specific meaning of the above terms may be understood by those of ordinary skill in the art according to the specific circumstances.
The animal hypoxia/reoxygenation culture device shown in figures 1-7 is applied to the simulation of animal hypoxia/reoxygenation survival conditions. If the hypoxia/reoxygenation condition is often involved in animal experiments, the oxygen concentration needs to be effectively regulated, the hypoxia/reoxygenation culture environment required by an animal model is created, the culture environment of a control group is ensured not to be interfered to the maximum extent, and the establishment of the hypoxia/reoxygenation culture condition of animals is realized.
The animal hypoxia/reoxygenation culture device mainly comprises an incubator 8, a control box 2 and a connection controller communicated with the incubator and the control box. The end face of the incubator 8 is in a right trapezoid shape, the incubator is divided into four identical culture cavities by three identical partition boards 18 which are identical to the end face of the incubator, each culture cavity is respectively provided with a culture drawer 17 which can be independently opened and closed, the culture drawer is similar to a drawer and is used for providing a single group of animal culture places, a food placing area 22 for placing food is arranged in each culture drawer so as to ensure the durability of animal culture, and a silica gel laminating layer 21 is arranged on the sealing contact surface of the culture drawer 17 and the incubator, so that the culture drawer is ensured to have tightness after being closed, and air leakage or animal escape is prevented.
The upper surface of the shell of the incubator 8 is provided with a transparent cover plate 19 with a certain thickness, which is convenient for observing and recording the animal culture process and is fixed at the top of each culture cavity in a slope shape. A feeding kettle 6 is arranged on the transparent cover plate, and the water outlet end of the feeding kettle 6 passes through a small hole penetrating through the transparent cover plate 19 and the culture suction box through a water delivery pipe to provide necessary water and nutrition for animals in the incubator.
The transparent cover plate 19, the shell of the incubator and the upper surface of the culture drawer box are provided with a first through pipe connecting hole 7, the lower part of the left side wall of the shell of the incubator is provided with a second through pipe connecting hole 9, the culture drawer box is provided with a culture drawer box air inlet hole 20 which is communicated with the second through pipe connecting hole 9, one end of a branch air inlet pipe penetrates through the second through pipe connecting hole 9 to be communicated with each culture drawer box 17, and the upper parts of the shell of the incubator and the right side wall of the culture drawer box are provided with a through exhaust hole 16.
The connection controller comprises an oxygen concentration detection conduit 5 connected to the first conduit connecting hole and communicated with the control box, a branch air inlet conduit 11 connected to the second conduit connecting hole and a main air inlet conduit 12, the oxygen concentration detection conduit realizes real-time adjustment of the oxygen concentration in the incubator, the main air inlet conduit is connected with each branch air inlet conduit 11 through a three-way connecting pipe 13, each branch air inlet conduit 11 is provided with an air inlet control valve 10, the air inlet control valves 10 are connected with the control box through wires 14 with a conduit 15 in a signal manner, and the air inlet control valves 10 are used for controlling the oxygen flow entering each culture pumping box.
An oxygen concentration sensor 3 for monitoring the oxygen concentration in each culture box in real time, an oxygen concentration controller 1 for controlling the opening of an air inlet control valve on the branch air inlet pipe and an oxygen concentration display screen 4 for displaying the oxygen concentration in the control box are arranged on the control box. The oxygen concentration sensor 3 monitors the oxygen concentration in the incubator in real time, the oxygen concentration controller 1 transmits control instructions sent by the oxygen concentration controller through wires in the wire pipes according to the set oxygen concentration requirement, the opening of an air inlet control valve on each branch air inlet pipe is regulated, and the regulation and the accurate control of the oxygen concentration in each culture pumping box are realized. The oxygen concentration display 4 may also set and operate an oxygen concentration controller.
When the animal hypoxia/reoxygenation culture device is used, small animals to be tested are respectively placed in each culture box 17, and the culture boxes 17 are inserted into the culture cavities separated by the partition boards. The water and nutrient substances are supplied to animals in the culture pumping boxes through the water supply kettle, foods for animal survival are placed in the food placement area, an oxygen concentration controller is set and controlled through the oxygen concentration display screen 4, oxygen is introduced into each culture pumping box 17 through the main air inlet pipe 12, the oxygen concentration in the culture pumping boxes is transmitted to the control box through the oxygen concentration detection pipe 5, the oxygen concentration in the control box is displayed through the oxygen concentration sensor 3, the oxygen concentration controller 1 transmits control instructions sent by the oxygen concentration controller through wires in the wire pipes according to the set oxygen concentration requirements and the oxygen concentration detected by the oxygen concentration sensor 3, and the opening degree of the air inlet control valve 10 on each branch air inlet pipe 11 is adjusted, so that the oxygen concentration in each culture pumping box is adjusted and accurately controlled.
The switching of the hypoxia/reoxygenation culture conditions in each culture pumping box can be realized rapidly by introducing different media (O 2 or N 2) into the main air inlet pipe.
Finally, it is noted that the above embodiments are only for illustrating the technical solution of the present invention and not for limiting the same, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications and equivalents may be made thereto without departing from the spirit and scope of the present invention, which is intended to be covered by the claims of the present invention.
Claims (5)
1. An animal hypoxia/reoxygenation culture device is characterized by comprising an incubator, a control box and a connection controller; wherein, more than two baffle plates which are the same as the end face of the incubator are arranged in the incubator and are separated into a plurality of culture cavities by the baffle plates, each culture cavity is respectively provided with a culture box which is independently opened and closed, the upper surface of the incubator is provided with a transparent cover plate which is arranged in a slope shape, and a feeding kettle which is used for feeding the animals in the culture box is arranged on the transparent cover plate;
The connection controller comprises an oxygen concentration detection conduit and a branch air inlet conduit which are communicated with each culture suction box and the control box, each branch air inlet conduit is communicated with the main air inlet conduit through a three-way connecting pipe, an air inlet control valve is arranged on each branch air inlet conduit, and the air inlet control valve is in signal connection with the control box through a wire with a wire conduit;
An oxygen concentration sensor for monitoring the oxygen concentration in each culture pumping box in real time and an oxygen concentration controller for controlling the opening of an air inlet control valve on the branch air inlet pipe are arranged on the control box;
a silica gel laminating layer is arranged on the contact surface of the culture drawer and the incubator;
A food placing area for placing food is arranged in each culture drawer box;
the transparent cover plate and the top of the culture drawing box are provided with through small holes, and the water outlet end of the water feeding kettle is fixedly provided with a water delivery pipe with a free end penetrating through the small holes.
2. The animal hypoxia/reoxygenation culture apparatus of claim 1, wherein the transparent cover plate, the shell of the incubator and the upper surfaces of the culture boxes are provided with first through pipe connecting holes, one end of the oxygen concentration detection pipe penetrates through the first pipe connecting holes to be communicated with each culture box, the lower part of the left side wall of the shell of the incubator is provided with second pipe connecting holes, the culture boxes are provided with culture box air inlet holes communicated with the second pipe connecting holes, one end of the branch air inlet pipe penetrates through the second pipe connecting holes to be communicated with each culture box, and the shell of the incubator and the upper part of the right side wall of the culture boxes are provided with through air outlet holes.
3. The animal hypoxia/reoxygenation culture device of claim 1, wherein the control box is provided with an oxygen concentration display screen for displaying the oxygen concentration in the control box.
4. The animal hypoxia/reoxygenation culture apparatus of claim 1, wherein the end surface of the incubator has a right angle trapezoidal shape.
5. The animal hypoxia/reoxygenation culture device of claim 2, wherein the vent is fixedly mounted with a one-way vent valve.
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| CN202010623099.6A CN111685051B (en) | 2020-07-01 | 2020-07-01 | Animal hypoxia/reoxygenation culture device |
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| CN202010623099.6A CN111685051B (en) | 2020-07-01 | 2020-07-01 | Animal hypoxia/reoxygenation culture device |
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| CN111685051B true CN111685051B (en) | 2024-06-04 |
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