CN207418760U - For the attached cell culture apparatus of polytype breast cancer cell synchronization dosing - Google Patents
For the attached cell culture apparatus of polytype breast cancer cell synchronization dosing Download PDFInfo
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- CN207418760U CN207418760U CN201721476333.7U CN201721476333U CN207418760U CN 207418760 U CN207418760 U CN 207418760U CN 201721476333 U CN201721476333 U CN 201721476333U CN 207418760 U CN207418760 U CN 207418760U
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- dosing
- breast cancer
- feed pipe
- polytype
- chemical feed
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Abstract
The utility model discloses a kind of attached cell culture apparatuses for polytype breast cancer cell synchronization dosing；Including chemical feed pipe, drug vial, mostly logical plastic tube and multiple bend neck blake bottles；There are one mostly logical plastic tube tools entrance and multiple outlets, multiple outlets are connected respectively by the way that medicine feeding needle is corresponding with multiple bend neck blake bottles, and entrance is connected by chemical feed pipe with drug vial；Chemical feed pipe is equipped with dosing filter device and chemical feed pipe switchs.The utility model helps to intervene the same acute drug of polytype breast cancer cell addition, it can guarantee dosing process control, synchronization, sterilization, at the uniform velocity change, it additionally aids and direct impact of the drug to cell is reduced during dosing, it can realize the appropriate culture for polytype breast cancer cell, be more conducive to observe cell routine and cell is kept to be in adhered state；Have the characteristics that it is easy to operate, highly practical, avoid pollution.
Description
Technical field
The utility model is related to technical field of cell culture, and in particular to one kind is synchronous for polytype breast cancer cell
The attached cell culture apparatus of dosing.
Background technology
Breast cancer (breast cancer) is a kind of one of malignant tumour for being happened at mammary gland galandular epithelium tissue and one
One of kind of highest tumour of the female malignant death rate, it was reported that the incidence of women with breast cancer is about in developing country
The 23% of women whole body malignant tumour is accounted for, the death rate of women with breast cancer accounts for 14% (Zhou Ye, Deng of women whole body malignant tumour
New Na, Fan Xiaoyan wait mastocarcinoma genes current treatment status and progress [J] translational medicine e-magazines, 2017,4 (9)：78-
83.) weight that the cause of disease of breast cancer, pathology, pathogenesis, treatment etc. are developing country's female malignants, therefore, is studied
Want one of problem.
At present, the cause of disease of breast cancer, pathology, pathogenesis are still not clear, while the therapy of breast cancer mainly includes
Chemotherapy (fMRI research [D] the China Science ＆ Technology University that Wang Lu chemotherapy influences patient with breast cancer's perform function,
2016.), (Li Jing, Lin Yifan, Xu Ying wait Mammary Cancer Treated by Chinese Medicine for radiation cure, operative treatment, Chinese medicine
Clinical progress [J] Liaoning Journal of Traditional Chinese Medicine, 2017,44 (9)：1994-1997.), acupuncture and moxibustion therapy etc., but breast cancer
Therapeutic effect is often not fully up to expectations, in particular for three cloudy breast cancer and advanced breast cancer (Dong Yi, Li Xiangpan, Song Qibin
.CDK4/6 inhibitor combined endocrine therapy advanced breast cancer progress [J] oncology magazines, 2017,23 (9)：807-
811.) therapeutic effect is extremely bad, and vast clinical medical personnel or basic researchers carry out new anti-breast cancer as a result,
The research of drug is very urgent.
But how vast clinical medical personnel or basic researchers carry out the research of new anti-breast cancer medicines
This just needs vast clinical medical personnel or the different types of breast cancer cell of basic researchers' in vitro culture, uses
New anti-breast cancer medicines are application factor, and using different types of breast cancer cell, main detection is new anti-for process object
Breast cancer medicines are to the effect of different types of breast cancer cell.
New anti-breast cancer medicines in order to active and effective are observed to different types of breast cancer cell line mcf-7, MDA-
MB-231, MDA-MB-468, MDA-MB-435 etc.) effect, it is necessary to be suitble to synchronization process different types of mammary gland
The adhere-wall culture bottle of cancer cell, and existing adhere-wall culture bottle cannot synchronize, the different types of breast of sterilization, controllableization processing
Adenocarcinoma cell and easily there is during different types of breast cancer cell dosing bacterium, virus, mycoplasma, Chlamydia, true
Bacterium etc. pollution condition still lacks new adherent training of the new anti-breast cancer medicines to different types of breast cancer cell at present
Bottle is supported, in order to study during different types of breast cancer cell culture dosing dosing synchronization, dosing is controllably changed, avoids dirt
Dye situation etc. correlation problem, there is an urgent need to develop the adhere-wall culture bottles for going out new different types of breast cancer cell.
The content of the invention
For in the prior art the defects of, the purpose of this utility model is to provide one kind convenient for user to a variety of mammary gland
Cancer cell dosing is realized dosing process control, synchronization, sterilization, is at the uniform velocity changed, helps to reduce drug during dosing
Direct impact to polytype breast cancer cell, additionally aiding prevents the thin for polytype breast cancer of cell attachment death
The attached cell culture apparatus of born of the same parents' synchronization dosing.
To achieve the above object, the utility model uses following technical scheme：
A kind of attached cell culture apparatus for polytype breast cancer cell synchronization dosing, which is characterized in that including：
One chemical feed pipe, a drug vial lead to plastic tube and multiple bend neck blake bottles more one；More logical plastic tube tools
There are one mostly logical plastics tube inlet and multiple more logical plastic tube outlets, the multiple more logical plastic tube outlets pass through medicine feeding needle respectively
Corresponding with multiple bend neck blake bottles to be connected, more logical plastics tube inlets are connected by the chemical feed pipe with the drug vial；Institute
Chemical feed pipe is stated to switch equipped with dosing filter device and chemical feed pipe.
Wherein, the chemical feed pipe includes dosing tube inlet, dosing buffering area, the chemical feed pipe switch and dosing being sequentially connected
Filter device；The dosing tube inlet is connected with the drug vial, and the dosing filter device is connected with more logical plastic tubes；
Between dosing tube inlet and dosing buffering area, if there are one ventilation connecting tubes, the end of the ventilation connecting tube is air filtration
Device.
Wherein, the dosing filter device include the first dosing filter device and the second dosing filter device, described first
Chemicals dosing plant is filtered to be arranged on than the second filtering chemicals dosing plant closer on the direction of drug vial；In the first filtering chemicals dosing plant
Equipped with the composite fibre film for possessing 0.45 μm of micropore；The mixing for possessing 0.22 μm of micropore is equipped in the second filtering chemicals dosing plant
Tunica fibrosa.
Wherein, the chemical feed pipe switch includes the dosing tube valve being arranged on the chemical feed pipe and on dosing tube valve
Plastics knob.
Wherein, more logical plastic tubes include leading to plastics tube inlet one more and lead to plastic tube outlet more four；Described
Leading to plastic tube outlet four respectively with four bend neck blake bottles to be connected more.
Wherein, more logical plastic tube outlets are connected tubule by second with the medicine feeding needle and are connected；The chemical feed pipe with
More logical plastics tube inlets are connected by the first connection tubule；The second connection tubule is equipped with spring clip；Described is more
Logical plastic tube outlet is connected the junction of tubule, the junction of the second connection tubule and medicine feeding needle, the first connection tubule with second
The junction for being connected tubule with first with the junction of the entrance of more logical plastic tubes and chemical feed pipe is equipped with plastic seal adhesive tape.
Wherein, the bend neck blake bottle is equipped with bend neck culture bottle cap；The bend neck culture bottle cap center is equipped with circle
The latex springhole of shape；The medicine feeding needle is deep into through the latex springhole in bend neck blake bottle.
Wherein, a chemical feed pipe, a drug vial, leading to plastic tube and multiple bend neck blake bottles one more and all setting
In in an incubator；
The incubator has incubator bottom plate, and the bend neck blake bottle is installed on incubator bottom plate；Bend neck blake bottle
Including body and bottleneck, the central axes of the bottleneck and the angle of incubator bottom plate are 13 °.
Wherein, the medicine feeding needle includes the head, body portion and the afterbody three parts that are sequentially connected；The head is equipped at least 20
A medicine feeding hole；The body portion is equipped with medicine feeding needle needle handle；The afterbody is connected tubule with second and is connected, and the second connection tubule with leading to more
The outlet of plastic tube is connected.
Wherein, a chemical feed pipe, a drug vial, leading to plastic tube and multiple bend neck blake bottles one more and all setting
In in an incubator；
The incubator has culture roof box；The outer surface of drug vial is equipped with drug vial scale, and the afterbody of drug vial is set
There is hanging ring, hanging ring is hung on by plastics fishing without hook on culture roof box.
Compared with prior art, the beneficial benefit of the utility model is：Help to add in polytype breast cancer cell
Same acute drug is intervened, and is ensured dosing process control, synchronization, sterilization, is at the uniform velocity changed, additionally aids in dosing
Drug is reduced in the process to the direct impact of polytype breast cancer cell and can be realized for polytype breast cancer cell
Appropriate culture, be more conducive to polytype breast cancer cell normal observation and polytype breast cancer cell kept to be in patch
Wall-like state.
Description of the drawings
Fig. 1 is the structure diagram of one embodiment of the utility model.
Fig. 2 is the structure diagram on medicine feeding needle head in the present embodiment.
Fig. 3 is the structure diagram of bend neck culture bottle cap in the present embodiment.
Reference sign：1- is directed to the attached cell culture apparatus of polytype breast cancer cell synchronization dosing；2- adds
Medicine bottle；The mostly logical plastic tubes of 3-；4- bend neck blake bottles；5- entrances；6- is exported；7- medicine feeding needles；8- chemical feed pipes；9- dosing tube inlets；
10- dosing buffering areas；11- chemical feed pipes switch；12- the first dosing filter devices；13- the second dosing filter devices；14- ventilations connect
It takes over；15- air filters；16- dosing tube valves；17- plastics knobs；The connection tubules of 18- second；19- spring clips；20-
First connection tubule；21- plastic seal adhesive tape；22- bend neck culture bottle caps；23- latex springholes；24- incubators；25- bottles
Body；26- bottlenecks；27- heads；28- bodies portion；29- afterbodys；30- medicine feeding holes；31- medicine feeding needle needle handles；32- bottom surfaces；33- drug vials
Scale value；34- hanging rings；35- plastics fishing without hooks；36- incubator bottom plates；37- cultivates roof box；A- angles.
Specific embodiment
As shown in Figure 1, the attached cell for polytype breast cancer cell synchronization dosing in the present embodiment cultivates dress
1 is put, including：Chemical feed pipe 8, drug vial 2, mostly logical plastic tube 3 and multiple bend neck blake bottles 4；There are one with adding for the mostly logical tool of plastic tube 3
The entrance 5 and multiple outlets 6 that pencil is connected, multiple outlets 6 are connected respectively by the way that medicine feeding needle 7 is corresponding with multiple bend neck blake bottles 4,
Entrance 5 is connected by chemical feed pipe 8 with drug vial 2；Chemical feed pipe 8 include be sequentially connected dosing tube inlet 9, dosing buffering area 10,
Chemical feed pipe switchs the 11, first dosing filter device 12 and the second dosing filter device 13；Dosing tube inlet 9 and dosing buffering area 10
Between, if there are one the ventilation connecting tubes 14 for playing ventilation, the end of ventilation connecting tube 14 is air filter 15；Dosing
Tube inlet 9 is connected with drug vial 2.
The composite fibre film for possessing 0.45 μm of micropore is equipped in first filtering chemicals dosing plant 12；Second filtering chemicals dosing plant 13
It is interior to be equipped with the composite fibre film for possessing 0.22 μm of micropore.
Chemical feed pipe switch 11 includes the dosing tube valve 16 being arranged on chemical feed pipe and the plastics on dosing tube valve 16
Knob 17.
Mostly logical plastic tube 3 includes an entrance 5 and four outlets 6；Four outlet 6 respectively with four 4 phases of bend neck blake bottle
Even.
The outlet 6 of more logical plastic tubes 3 is connected tubule 18 by second with medicine feeding needle 7 and is connected；Chemical feed pipe 8 and mostly logical plastic tube
Entrance 5 on 3 is connected by the first connection tubule 20, and the second connection tubule 18 is equipped with spring clip 19；On mostly logical plastic tube 3
Outlet 6 with second be connected tubule 18 junction, second connect the junction of tubule 18 and medicine feeding needle 7, first be connected tubule 20
The junction for being connected tubule 20 with first with the junction of the entrance 6 of more logical plastic tubes 3 and chemical feed pipe 8 is equipped with plastic seal glue
Item 21.
Bend neck blake bottle 4 is equipped with bend neck culture bottle cap 22 as shown in Figure 3；22 center of bend neck culture bottle cap is equipped with
Circular latex springhole 23；Medicine feeding needle 7 is deep into through latex springhole 23 in bend neck blake bottle 4.Bend neck blake bottle 4 and askew
Neck culture bottle cap 22 is closely engaged by screw thread.
Further include an incubator 24, chemical feed pipe 8, drug vial 2, mostly logical plastic tube 3 and multiple bend neck blake bottles 4；It is all provided with
In incubator 24；Incubator 24 includes incubator bottom plate 36 and culture roof box 37.
4 lower section of bend neck blake bottle is equipped with incubator bottom plate 36；Bend neck blake bottle 4 includes body 25 and bottleneck 26, bottleneck 26
Central axes and incubator bottom plate 36 between angle a for 13 °, the area of the bottom surface 32 of bend neck blake bottle 4 is 70cm2。
As shown in Fig. 2, medicine feeding needle includes the head 27, body portion 28 and the afterbody 29 that are sequentially connected；Head 27 passes through latex bullet
Property hole 23 be deep into bend neck blake bottle 4, head 27 be equipped at least 20 medicine feeding holes 30；Body portion 28 is equipped with medicine feeding needle needle handle 31；
Afterbody 29 is connected tubule 18 with second and is connected, and the second connection tubule 18 is connected with the outlet 6 on more logical plastic tubes 3.
The outer surface of drug vial 2 is equipped with drug vial scale 33, and the afterbody of drug vial 2 is equipped with hanging ring 34, and hanging ring 34 passes through modeling
Material fishing without hook 35 is hung on culture roof box 37.
The utility model patent can carry out dosing to 4 kinds of different types of breast cancer cells simultaneously, such as using new
The different types of breast cancer cell line mcf-7 of anti-breast cancer medicines processing, MDA-MB-231, MDA-MB-468, MDA-MB-435,
It needs to cultivate breast cancer cell line mcf-7, breast carcinoma cell strain MDA-MB-231, mammary gland respectively in four bend neck blake bottles 4
Cancer cell line MDA-MB-468 and breast carcinoma cell strain MDA-MB-435.
If the breast cancer cell dosing in wherein some bend neck blake bottle 4 is not needed to screw plastics knob 17, and
The bend neck blake bottle 4 corresponding second connection tubule 18 is clamped using spring clip 19.
If plastics are unscrewed, it is necessary to install new anti-breast cancer medicines in drug vial 2 to multiple cell line synchronization dosings
Knob 17, trip spring folder 19, new anti-breast cancer medicines pass through drug vial 2 at this time, chemical feed pipe 8, first connects tubule 20, more
Logical plastic tube 3, second connects tubule 18, medicine feeding needle 7 enters cultivating system.
Claims (10)
1. a kind of attached cell culture apparatus for polytype breast cancer cell synchronization dosing, which is characterized in that including：
One chemical feed pipe, a drug vial lead to plastic tube and multiple bend neck blake bottles more one；More logical plastic tubes have one
A more logical plastics tube inlet and multiple more logical plastic tube outlets, the multiple more logical plastic tubes outlets respectively by medicine feeding needle with it is more
A bend neck blake bottle, which corresponds to, to be connected, and more logical plastics tube inlets are connected by the chemical feed pipe with the drug vial；It is described to add
Pencil is equipped with dosing filter device and chemical feed pipe switchs.
2. the attached cell culture apparatus according to claim 1 for polytype breast cancer cell synchronization dosing,
It is characterized in that：
The chemical feed pipe includes dosing tube inlet, dosing buffering area, the chemical feed pipe switch and dosing filter device being sequentially connected；
The dosing tube inlet is connected with the drug vial, and the dosing filter device is connected with more logical plastic tubes；Chemical feed pipe enters
Between mouth and dosing buffering area, if there are one ventilation connecting tubes, the end of the ventilation connecting tube is air filter.
3. the attached cell culture apparatus according to claim 1 or 2 for polytype breast cancer cell synchronization dosing,
It is characterized in that：
The dosing filter device includes the first dosing filter device and the second dosing filter device, the first dosing filtering dress
It is arranged in than the second dosing filter device closer on the direction of drug vial；It is equipped with and possesses in the first dosing filter device
The composite fibre film of 0.45 μm of micropore；The composite fibre film for possessing 0.22 μm of micropore is equipped in the second dosing filter device.
4. the attached cell culture apparatus according to claim 1 or 2 for polytype breast cancer cell synchronization dosing,
It is characterized in that：
The chemical feed pipe switch includes the dosing tube valve being arranged on the chemical feed pipe and the plastics rotation on dosing tube valve
Button.
5. the attached cell culture apparatus according to claim 1 for polytype breast cancer cell synchronization dosing,
It is characterized in that：
More logical plastic tubes include leading to plastics tube inlet one more and lead to plastic tube outlet more four；Described leads to modeling more four
Expects pipe outlet is connected respectively with four bend neck blake bottles.
6. the attached cell culture apparatus according to claim 1 for polytype breast cancer cell synchronization dosing,
It is characterized in that：
More logical plastic tube outlets are connected tubule by second with the medicine feeding needle and are connected；The chemical feed pipe and more logical modelings
Expects pipe entrance is connected by the first connection tubule；The second connection tubule is equipped with spring clip；The how logical plastic tube goes out
Mouth is connected the junction of tubule, the junction of the second connection tubule and medicine feeding needle, the first connection tubule with leading to plastics more with second
The junction that the junction of the entrance of pipe and chemical feed pipe are connected tubule with first is equipped with plastic seal adhesive tape.
7. the attached cell culture apparatus according to claim 1 for polytype breast cancer cell synchronization dosing,
It is characterized in that：
The bend neck blake bottle is equipped with bend neck culture bottle cap；The bend neck culture bottle cap center is equipped with circular latex bullet
Property hole；The medicine feeding needle is deep into through the latex springhole in bend neck blake bottle.
8. the attached cell culture apparatus according to claim 1 for polytype breast cancer cell synchronization dosing,
It is characterized in that：
One chemical feed pipe, a drug vial lead to plastic tube and multiple bend neck blake bottles are all arranged at a training more one
It supports in case；
The incubator has incubator bottom plate, and the bend neck blake bottle is installed on incubator bottom plate；Bend neck blake bottle includes
Body and bottleneck, the central axes of the bottleneck and the angle of incubator bottom plate are 13 °.
9. the attached cell culture apparatus for polytype breast cancer cell synchronization dosing according to claim 1 or 6,
It is characterized in that：
The medicine feeding needle includes the head, body portion and the afterbody three parts that are sequentially connected；The head is equipped at least 20 medicine feeding holes；
The body portion is equipped with medicine feeding needle needle handle；The afterbody is connected tubule with second and is connected, the second connection tubule and more logical plastic tubes
Outlet is connected.
10. the attached cell culture apparatus according to claim 1 for polytype breast cancer cell synchronization dosing,
It is characterized in that：
One chemical feed pipe, a drug vial lead to plastic tube and multiple bend neck blake bottles are all arranged at a training more one
It supports in case；
The incubator has culture roof box；The outer surface of drug vial is equipped with drug vial scale, and the afterbody of drug vial, which is equipped with, to be hung
Ring, hanging ring are hung on by plastics fishing without hook on culture roof box.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721476333.7U CN207418760U (en) | 2017-11-08 | 2017-11-08 | For the attached cell culture apparatus of polytype breast cancer cell synchronization dosing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721476333.7U CN207418760U (en) | 2017-11-08 | 2017-11-08 | For the attached cell culture apparatus of polytype breast cancer cell synchronization dosing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207418760U true CN207418760U (en) | 2018-05-29 |
Family
ID=62307941
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721476333.7U Expired - Fee Related CN207418760U (en) | 2017-11-08 | 2017-11-08 | For the attached cell culture apparatus of polytype breast cancer cell synchronization dosing |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207418760U (en) |
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2017
- 2017-11-08 CN CN201721476333.7U patent/CN207418760U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180529 Termination date: 20191108 |