CN207408306U - A kind of leaf chamber for being used to measure leaf cross-section chlorophyll fluorescence - Google Patents
A kind of leaf chamber for being used to measure leaf cross-section chlorophyll fluorescence Download PDFInfo
- Publication number
- CN207408306U CN207408306U CN201721348899.1U CN201721348899U CN207408306U CN 207408306 U CN207408306 U CN 207408306U CN 201721348899 U CN201721348899 U CN 201721348899U CN 207408306 U CN207408306 U CN 207408306U
- Authority
- CN
- China
- Prior art keywords
- leaf
- section
- leaf chamber
- blade
- chlorophyll fluorescence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 229930002875 chlorophyll Natural products 0.000 title claims abstract description 28
- 235000019804 chlorophyll Nutrition 0.000 title claims abstract description 28
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 title claims abstract description 28
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000000463 material Substances 0.000 claims abstract description 13
- 230000005540 biological transmission Effects 0.000 claims abstract description 3
- 238000007789 sealing Methods 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 238000002834 transmittance Methods 0.000 claims description 3
- 230000003287 optical effect Effects 0.000 abstract description 2
- 239000007789 gas Substances 0.000 description 15
- 230000000243 photosynthetic effect Effects 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000029553 photosynthesis Effects 0.000 description 3
- 238000010672 photosynthesis Methods 0.000 description 3
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 241001074085 Scophthalmus aquosus Species 0.000 description 1
- GCNLQHANGFOQKY-UHFFFAOYSA-N [C+4].[O-2].[O-2].[Ti+4] Chemical compound [C+4].[O-2].[O-2].[Ti+4] GCNLQHANGFOQKY-UHFFFAOYSA-N 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 239000000109 continuous material Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000005357 flat glass Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000003760 hair shine Effects 0.000 description 1
- 230000017525 heat dissipation Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Microscoopes, Condenser (AREA)
Abstract
The utility model is related to a kind of for measuring the leaf chamber of leaf cross-section chlorophyll fluorescence, which is characterized in that the leaf chamber includes leaf chamber body and blade presss from both sides；The leaf chamber body is the body structure being detachably connected by pedestal and upper cover, and the pedestal uses light transmissive material；The base top interval setting is useful for plugging two light transmission baffles of the fixed blade folder, and the base bottom is provided with photomask, offers to be convenient for the light hole for observing blade on the photomask；Through hole and check valve are provided on the upper lid, the through hole is used to plug the humidity sensor of fixed gas generating means, the check valve is used to connect the gas outlet of the gas generating unit, the upper cover lower part is additionally provided with to connect the light source opening of light supply apparatus, and the utility model can be widely applied in technical field of optical instrument.
Description
Technical field
The utility model is to belong to optical instrument skill on a kind of for measuring the leaf chamber of leaf cross-section chlorophyll fluorescence
Art field.
Background technology
Photosynthesis is one of some biology key that they depend on for existence biological processes on the earth, and Photosynthetic is by titanium dioxide
Carbon and water are converted into organic matter, release oxygen, while the luminous energy absorbed is changed into available chemical energy, and chlorophyll is glimmering
The probe that light is studied as photosynthesis, reflects the Photochemical Efficiency of Photosynthetic and the variation of heat dissipation ability, in blade
Difference mesophyll tissue of portion is different with light intensity absorption ability to different light medium.The chlorophyll fluorescence instrument of commercialization at present exists certain
Limitation (is only capable of measuring blade surface or the chlorophyll fluorescence of blade interior certain point), it is impossible to obtain the real light of mesophyll tissue
Conjunction ability, and the general small volume of fluorescence leaf chamber provisioned in the chlorophyll fluorescence instrument of commercialization are only capable of horizontal clamping blade, thoroughly
Unthreaded hole is in blade surface, it is impossible to the chlorophyll fluorescence that leaf cross-section is measured on microscope.
Using the chlorophyll fluorescence of high resolution microscope observation leaf cross-section, it can go deep into and systematically study blade
The difference of internal photosynthetic capacity, and currently used for measuring the easy device of leaf cross-section, it is general to be pressed from both sides using 2 glass slides
Cross section is observed towards microlens or uses U-shaped windowpane container, then clamped blade with 2 sheet glass gaskets and put by firmly blade
Enter in container and observe, however, such easy device is not avoided that the fluorescence pollution of blade surface, moisture can not be kept, caused
Make leaf cross-section fluoremetry inaccurate.
The content of the invention
In view of the above-mentioned problems, the purpose of this utility model is to provide a kind of fluoremetry accurately for measure blade transversal
The leaf chamber of face chlorophyll fluorescence.
To achieve the above object, the utility model takes following technical scheme：One kind is green for measuring leaf cross-section leaf
The leaf chamber of plain fluorescence, which is characterized in that the leaf chamber includes leaf chamber body and blade presss from both sides；The leaf chamber body is by pedestal and upper cover
The body structure being detachably connected, the pedestal use light transmissive material；The base top interval setting is useful for plugging
Two light transmission baffles of the fixed blade folder, the base bottom are provided with photomask, are offered on the photomask for just
In the light hole of observation blade；Through hole and check valve are provided on the upper lid, the through hole is used to plug fixed gas
The humidity sensor of device, the check valve are used to connect the gas outlet of the gas generating unit, and the upper cover lower part is also set
It is equipped with to connect the light source opening of light supply apparatus.
Preferably, the blade folder includes spring clip and gasket, and the spring clip includes two fold-line-shaped side plates, two foldings
The top plate opposite side of linear side plate is fixedly connected with spring, and each fold-line-shaped side plate interior sides are fixedly installed one for sandwiched leaf
The gasket of piece.
Preferably, the two fold-line-shaped side plates are using light transmissive material, and two gaskets are using lighttight black sea
Continuous material.
Preferably, the bottom of two gaskets and the bottom of the two fold-line-shaped side plates are described in same horizontal line or two
The bottom of gasket is higher than 1~2mm of bottom of the two fold-line-shaped side plates.
A fan is fixedly installed in the leaf chamber bodies top for being preferably located at the blade folder top.
Preferably, it is provided with sealing ring between the pedestal and upper cover.
Preferably, the light source opening uses slit shape structure.
Preferably, the pedestal is more than 95% silica glass material using light transmittance.
Preferably, the distance between two described baffles are not less than 30mm.
Preferably, the leaf chamber body uses the body structure of 10cm*10cm*6cm, and the height of the blade folder is not more than
50mm。
The utility model has the following advantages due to taking above technical scheme：1st, the blade folder of the utility model is
It is made of the spring clip using light transmissive material and the gasket using light tight absorbent material, blade is mounted on two on the inside of spring clip
Between gasket, installation is simple and fast, and after being passed through gas in leaf chamber body, bibulous gasket can ensure Blade measuring process
In always in moisture state, prevent leaves water loss from chloroplaset being caused to inactivate, while ensure the upper and lower surface of blade only close to aobvious
The part irradiation of objective avoids the fluorescence at other positions of blade from polluting, improves fluorimetric accuracy.2nd, this practicality is new
Type can ensure that the incident light of micro objective only irradiates leaf cross-section, prevent due to being provided with photomask in base bottom
The fluorescence pollution of blade upper and lower surface.3rd, it can need to set according to experiment in the leaf chamber body of the utility model and plug fixed gas
The humidity sensor of body generating means, upper cover can connect external light source device by light source interface and be passed through external light source, and lead to
The gas outlet for crossing check valve connection gas generating unit is passed through gas needed for experiment, and the leaf chamber body of box sealing can be according to aobvious
Micro mirror objective table carries out respective design, has very high flexibility.4th, fan is additionally provided in the leaf chamber body of the utility model,
Gas needed for experiment can be made equably to be full of in leaf chamber body, and then the cross section of blade is enable fully to carry out photosynthetic work
With the raising fluorimetric accuracy of leaf cross-section can be widely applied in photosynthetic instrument field.
Description of the drawings
Fig. 1 is the structure diagram of the utility model leaf chamber；
Fig. 2 is the structure diagram of the utility model blade folder；
Fig. 3 is using the utility model and the knot using existing easy device measure leaf cross-section chlorophyll fluorescence yield
Fruit comparison diagram, wherein, "-" represents the leaf cross-section chlorophyll fluorescence yield measured using the utility model, and "-" represents to use
The leaf cross-section chlorophyll fluorescence yield that existing easy device measures.
Specific embodiment
Come to carry out the utility model detailed description below in conjunction with attached drawing.It should be appreciated, however, that attached drawing is provided only
More fully understand the utility model, they should not be understood the limitation of paired the utility model.
As shown in Figure 1, provided by the utility model include leaf chamber for measuring the leaf chamber of leaf cross-section chlorophyll fluorescence
Body 1, blade folder 2, baffle 3, photomask 4, fan 5 and check valve 6, wherein, leaf chamber body 1 includes pedestal 11 and upper cover 12,
Pedestal 11 and baffle 3 use light transmissive material.
11 top of pedestal is detachably connected upper cover 12 by screw and forms leaf chamber body 1, and leaf chamber body 1 uses babinet knot
Structure, 11 top of pedestal are also arranged at intervals two baffles 3 for being useful for plugging fixed blade folder 2, and 11 bottom of pedestal is provided with photomask
4, the light hole that microscope 7 is convenient for observe blade 8 is offered on photomask 4.Leaf chamber body 1 above blade folder 2
Fan 5 is fixedly installed in top, and 12 top of upper cover offers through hole 13, for plugging the humidity sensor of fixed gas generating means
Device, 12 top of upper cover also plug fixed check valve 6, and for connecting the gas outlet of gas generating unit, 12 lower part both sides of upper cover are equal
Light source opening 14 is offered, for connecting light supply apparatus, wherein, gas generating unit and light supply apparatus are the prior art,
This is not repeated.
As shown in Fig. 2, blade folder 2 includes spring clip 21 and gasket 22, spring clip 21 includes two fold-line-shaped side plates 23 and one
Spring 24, the top plate opposite side of each fold-line-shaped side plate 23 are fixedly connected with spring 24, and each 23 inside of fold-line-shaped side plate is fixed
One gasket 22 for being used for sandwiched blade 8 is set, the bottoms of the bottom of two gaskets 22 and two fold-line-shaped side plates 23 in same horizontal line,
Or two gasket 22 bottom be higher than two fold-line-shaped side plates 23 1~2mm of bottom.Each gasket 22 may be employed lighttight black
Color sponge material, for keeping the moistening of blade 8 light tight, each fold-line-shaped side plate 23 uses light transmissive material.
In a preferred embodiment, sealing ring is additionally provided between pedestal 11 and upper cover 12, for ensureing leaf chamber sheet
The air-tightness of body 1.
In a preferred embodiment, pedestal 11 is more than 95% silica glass material using light transmittance.
In a preferred embodiment, light source opening 14 uses slit shape structure.
In a preferred embodiment, the size of leaf chamber body 1 can be carried out according to 7 objective table upper space of microscope
Setting, such as：Leaf chamber body 1 is using the body structure of 10cm*10cm*6cm, and the height of blade folder 2 is no more than 50mm.
In a preferred embodiment, the distance between two baffles 3 are not less than 30mm.
It is new that this practicality is described in detail using existing steam generator and inverted fluorescence microscope as specific embodiment below
Type is used to measure the use process of the leaf chamber of leaf cross-section chlorophyll fluorescence：
1) pedestal 11 is fixed on the objective table of inverted fluorescence microscope；
2) selection needs the fresh plant leaf observed, and avoids the blade that vein part cuts out 1cm*1cm sizes with blade
8；
3) gasket 22 with water is bedewed, and between the blade 8 cut is fixed on two gaskets 22, makes the cross section of blade 8
Edge keeps flushing with two gaskets, 22 bottom；
4) cross section of blade 8 will be made towards the object lens of inverted fluorescence microscope in 2 insertion baffle 3 of blade folder；
5) upper cover 12 and pedestal 11 are fixed by screws into leaf chamber body 1, and check the airtight of leaf chamber body 1
Property；
6) gas outlet of steam generator is connected into check valve 6, opens steam generator, water vapour is passed through
In leaf chamber body 1, opening fan 5 makes water vapour be uniformly filled in leaf chamber body 1；
7) humidity for setting humidity sensor by the moisture control function of steam generator is more than 70%, and will be wet
Spend in sensor insertion through hole 13, adjust the gas flow of steam generator and duration of ventilation make it is wet in leaf chamber body 1
Degree reaches requirement of experiment；
8) light supply apparatus is opened, external light source, which enters through light source opening in leaf chamber body 1, shines the cross section of blade 8
It penetrates, the cross section of blade 8 proceeds by photosynthesis；
9) inverted fluorescence microscope is adjusted, the cross section mesophyll tissue for making blade 8 is high-visible, to 8 cross section of blade
Chlorophyll fluorescence yield is measured.
As shown in figure 3, it is apparent that compared with using the easy device for measuring blade 8 cross section, using this practicality
New leaf chamber is measured the chlorophyll fluorescence yield of 8 cross section of blade the fluorescence pollution that can reduce by 8 surface of blade, protects
8 moisture of blade is held, makes fluorescence measurement more accurately and reliably.
The various embodiments described above are merely to illustrate the utility model, wherein the structure of each component, connection mode and manufacture craft
Deng can all be varied from, every equivalents carried out on the basis of technical solutions of the utility model and improvement,
It should not exclude outside the scope of protection of the utility model.
Claims (10)
- It is 1. a kind of for measuring the leaf chamber of leaf cross-section chlorophyll fluorescence, which is characterized in that the leaf chamber include leaf chamber body and Blade presss from both sides；The leaf chamber body is the body structure being detachably connected by pedestal and upper cover；The base top interval setting is useful for plugging two light transmission baffles of the fixed blade folder, and the base bottom is set There is photomask, offer to be convenient for the light hole for observing blade on the photomask；Through hole and check valve are provided on the upper lid, the through hole is used to plug the humidity sensor of fixed gas generating means Device, the check valve are used to connect the gas outlet of the gas generating unit, and the upper cover lower part is additionally provided with to connect light The light source opening of source device.
- 2. a kind of leaf chamber for being used to measure leaf cross-section chlorophyll fluorescence as described in claim 1, which is characterized in that described Blade folder includes spring clip and gasket, and the spring clip includes two fold-line-shaped side plates, and the top plate of the two fold-line-shaped side plates is opposite Side is fixedly connected with spring, and a gasket for sandwiched blade is fixedly installed in each fold-line-shaped side plate interior sides.
- A kind of 3. leaf chamber for being used to measure leaf cross-section chlorophyll fluorescence as claimed in claim 2, which is characterized in that two institutes Fold-line-shaped side plate is stated using light transmissive material, two gaskets use lighttight black sponge material.
- A kind of 4. leaf chamber for being used to measure leaf cross-section chlorophyll fluorescence as claimed in claim 2, which is characterized in that two institutes The bottom of gasket is stated with the bottom of the two fold-line-shaped side plates in the bottom of same horizontal line or two gaskets to be higher than described in two 1~2mm of bottom of fold-line-shaped side plate.
- 5. a kind of leaf chamber for being used to measure leaf cross-section chlorophyll fluorescence as described in claim 1, which is characterized in that be located at A fan is fixedly installed in the leaf chamber bodies top of the blade folder top.
- 6. a kind of leaf chamber for being used to measure leaf cross-section chlorophyll fluorescence as described in claim 1, which is characterized in that described Sealing ring is provided between pedestal and upper cover.
- 7. a kind of leaf chamber for being used to measure leaf cross-section chlorophyll fluorescence of such as claim 1 to 6 any one of them, feature It is, the light source opening uses slit shape structure.
- 8. a kind of leaf chamber for being used to measure leaf cross-section chlorophyll fluorescence of such as claim 1 to 6 any one of them, feature It is, the pedestal is more than 95% silica glass material using light transmittance.
- 9. a kind of leaf chamber for being used to measure leaf cross-section chlorophyll fluorescence of such as claim 1 to 6 any one of them, feature It is, the distance between two described baffles are not less than 30mm.
- It is 10. special if claim 1 to 6 any one of them is a kind of for measuring the leaf chamber of leaf cross-section chlorophyll fluorescence Sign is that the leaf chamber body uses the body structure of 10cm*10cm*6cm, and the height of the blade folder is not more than 50mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721348899.1U CN207408306U (en) | 2017-10-19 | 2017-10-19 | A kind of leaf chamber for being used to measure leaf cross-section chlorophyll fluorescence |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721348899.1U CN207408306U (en) | 2017-10-19 | 2017-10-19 | A kind of leaf chamber for being used to measure leaf cross-section chlorophyll fluorescence |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207408306U true CN207408306U (en) | 2018-05-25 |
Family
ID=62320307
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721348899.1U Expired - Fee Related CN207408306U (en) | 2017-10-19 | 2017-10-19 | A kind of leaf chamber for being used to measure leaf cross-section chlorophyll fluorescence |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207408306U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109142218A (en) * | 2018-07-16 | 2019-01-04 | 西北农林科技大学 | A method of utilizing photosynthesis measurement system measurement different times vegetable seeds, fruit photosynthesis |
| CN114646621A (en) * | 2022-03-21 | 2022-06-21 | 江苏大学 | Leaf in-situ dark adaptation device and method for chlorophyll fluorescence monitoring |
-
2017
- 2017-10-19 CN CN201721348899.1U patent/CN207408306U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109142218A (en) * | 2018-07-16 | 2019-01-04 | 西北农林科技大学 | A method of utilizing photosynthesis measurement system measurement different times vegetable seeds, fruit photosynthesis |
| CN114646621A (en) * | 2022-03-21 | 2022-06-21 | 江苏大学 | Leaf in-situ dark adaptation device and method for chlorophyll fluorescence monitoring |
| WO2023179354A1 (en) * | 2022-03-21 | 2023-09-28 | 江苏大学 | Leaf in-situ dark adaptation device and method for chlorophyll fluorescence monitoring |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180525 |