CN109142218A - A method of utilizing photosynthesis measurement system measurement different times vegetable seeds, fruit photosynthesis - Google Patents
A method of utilizing photosynthesis measurement system measurement different times vegetable seeds, fruit photosynthesis Download PDFInfo
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- CN109142218A CN109142218A CN201810778285.XA CN201810778285A CN109142218A CN 109142218 A CN109142218 A CN 109142218A CN 201810778285 A CN201810778285 A CN 201810778285A CN 109142218 A CN109142218 A CN 109142218A
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- leaf chamber
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- reticular structure
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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Abstract
The present invention provides a kind of method using photosynthesis measurement system measurement different times vegetable seeds, fruit photosynthesis.This method increases an auxiliary leaf chamber in existing 6400-02 or -02B LED light source leaf chamber, after silique to be determined is cut, is placed in auxiliary lobe room intracavity bottom or is sandwiched in leaf chamber slot in parallel, closes LED light source leaf chamber, carries out the measurement such as photosynthesis.The production of auxiliary leaf chamber used in this method is simple, easy to use.It can measure the photosynthesis of siliqua of oilseed rape and other vegetable seeds, fruit using this method, it is easy to operate, be widely used, accurately and reliably.
Description
Technical field
The present invention relates to photosynthesis of plant to measure system, and in particular to a kind of measurement vegetable seeds or fruit photosynthesis
Method.
Background technique
LI-6400 (XT) photosynthesis measurement system of LI-COR company production is current internationally recognized Plant Light cooperation
With determining instrument, principle is the CO by consuming during measurement photosynthesis of plant2It measures to calculate the net photosynthesis of plant speed
Rate.It is different since the plant variety of nature is various, so the leaf chamber of photosynthesis measurement system can not be applicable at present
In measurement in need plant sample.
In existing photosynthesis measurement system, band light source leaf chamber is rectangle (20mm × 30mm), and diameter is greater than
1mm, length are greater than the cylindricality (for example, siliqua of oilseed rape) or irregular type plant sample of 30mm, can not be closed by upper and lower leaf chamber
When gasket deformation sample is closed in upper and lower lobes room, thus closed gas-flow channel needed for sample detection can not be formed；
Alternatively, small sample is crossed less than 20mm for length, because that can not be fixed in leaf chamber, to be caused by air-flow sucking air flue
Block air flue, damage measurement system.Therefore, the CO that the plant sample of the above classification consumes in photosynthesis2It can not accurately examine
It surveys, i.e., the shape of leaf chamber and structure limit photosynthesis measurement systematic difference range.
Summary of the invention
Photosynthesis measurement system measurement different times vegetable seeds, fruit are utilized the purpose of the present invention is to provide a kind of
Photosynthetic method.
In order to achieve the above objectives, the invention adopts the following technical scheme:
A kind of auxiliary leaf chamber, it is described including the elastic material block to match with photosynthesis measurement system leaf chamber shape
The upper surface of elastic material block is provided with leaf chamber slot and the first reticular structure layer, the lower surface of elastic material block are provided with
For indent in the intracorporal card slot of elastic material block, the top surface of card slot is provided with the second reticular structure layer, the first reticular structure layer and
It is provided between two reticular structure layers through the inner cavity of elastic material block；Leaf chamber slot is including symmetry arrangement two-by-two in elastic material
The crack of block upper surface lumen openings both sides of the edge.
Preferably, the elastic material block is made of EVA foam.
Preferably, the first reticular structure layer and the second reticular structure layer are made of nylon wire.
Preferably, the nylon wire is 80-120 mesh.
Preferably, the first reticular structure layer and the second reticular structure layer are white, and elastic material block is black.
A kind of photosynthesis measurement system, including above-mentioned auxiliary leaf chamber, the auxiliary leaf chamber are fixed on photosynthetic by its card slot
It acts on the indoor cyclic sealing gasket of measurement system leaf, the hollow space of the gasket is opposite with the auxiliary inner cavity of leaf chamber.
A method of measurement different times vegetable seeds, fruit photosynthesis, comprising the following steps:
1) block to match with photosynthesis measurement system leaf chamber shape is processed using elastic material；In block
Portion's processing runs through the inner cavity of block upper and lower surfaces；In block upper surface, the both sides of the edge of lumen openings process symmetry arrangement two-by-two
Crack；Processing can cover the counterbore of the open edge at the lumen openings of block lower surface；Process the first reticular structure and
Second reticular structure is fixed in counterbore by two reticular structures, and covering blocks lower surface lumen openings；
2) vegetable seeds or fruit are put into inner cavity and are laid on the second reticular structure, or by vegetable seeds or fruit
Both ends are embedded in and are fixed in crack, so that vegetable seeds or fruit are across block upper surface lumen openings；Then by the first net
Shape structure is covered and is fixed at the lumen openings of block upper surface；
3) after step 2), using counterbore as card slot, block (built-in vegetable seeds, fruit) is fixed on by counterbore
On the indoor cyclic sealing gasket of photosynthesis measurement system leaf, keep the hollow space of the gasket opposite with inner cavity；
4) close photosynthesis measurement system leaf chamber, by photosynthesis measurement system leaf chamber closing process to block
Squeezing action makes the inner cavity for assisting leaf chamber form closed airflow channel；
5) LED light source for opening photosynthesis measurement system leaf chamber, is automatically obtained photosynthetic using photosynthesis measurement system
Data；
6) the total projection area of leaf area vegetable seeds or the tested part of fruit in photosynthetic data is replaced, thus right
Photosynthetic data are modified；
7) Physiological Index of Photosynthesis of vegetable seeds or fruit is calculated according to revised photosynthetic data.
Preferably, the vegetable seeds or fruit are tested the acquisition methods of the total projection area of part are as follows: are swept using root system
It retouches system measurement vegetable seeds or fruit is located at the total projection area of inner cavity point；Alternatively, acquisition inner cavity image, using washing root
Measurement analysis software calculates the total projection area of vegetable seeds or fruit in the image.
Preferably, the physical signs includes photosynthetic rate, stomatal conductance, intercellular CO2Concentration and transpiration rate.
The beneficial effects of the present invention are embodied in:
Auxiliary leaf chamber of the present invention utilize reticular structure, elastic material block and leaf chamber slot, solve cylindricality or other
Irregular shape plant sample (especially seed, fruit) is not easy the problem of loading in photosynthesis measurement system, ensure that
Leaf chamber air-tightness, structure, production are simple, easy to use, are widely used, and are guaranteeing the original performance of photosynthesis measurement system instrument
With global shape it is constant in the case where, expand photosynthesis measurement systematic difference range, improve the accurate of measurement result
Degree.
It further,, can be with using the nylon mesh material and EVA foam of certain mesh number in auxiliary leaf chamber of the invention
Sample loss is prevented, the test object scope of application is improved and fast, easily forms closed good detection airflow channel.
The present invention is modified by designing and producing auxiliary leaf chamber, and to photosynthetic data, is surveyed not changing photosynthesis
In the case where determining basic operation process, provided for the physiological status and photosynthesis of follow-up study rape different times silique growth
One reliable physical signs detection method, this method can also be used for detecting the seed or fruit of other plants, such as: sesame,
The awn of wheat of the pod of silk tree, beans etc. and wheat, rice spike of rice etc. photosynthesis.
Detailed description of the invention
Fig. 1 is auxiliary leaf chamber sectional view；
Fig. 2 is auxiliary leaf chamber stereoscopic schematic diagram；
Fig. 3 is auxiliary leaf chamber scheme of installation；
In figure: 1. nylon wires, 2. elastomer blocks, 3. leaf chamber slots, 4. card slots, leaf chamber, 6. auxiliary leaf chambers, 7. lower leaf chambers are close on 5.
Packing, 8. lower leaf chambers, 9.LED light source, 10. infrared analyzers, 11 hosts.
Specific embodiment
The present invention is described in further details with reference to the accompanying drawings and examples.
The present invention proposes a kind of detection oil using LI-6400 (XT) photosynthesis measurement system of LI-COR company production
The photosynthetic method of dish different times silique can be used for measuring fruit, seed of siliqua of oilseed rape and similar shape etc. and plant
The photosynthesis of object sample.
Rape is as a kind of oil crops, and within the scope of 10-120mm, thickness exists the Pod length of different growing stage
Change within the scope of 1-7mm.To complete detection, the present invention increases by one in existing 6400-02 or -02B LED light source leaf chamber
Assist leaf chamber.
Referring to Fig. 1 and Fig. 2, assist leaf chamber that there is the inner cavity, openable first Buddhist nun that ventilation is good, air-flow is not hindered to pass through
Imperial net and the second fixed nylon wire, and light transmittance is good, can carry tested silique weight, tested silique must not be in air-flow band
Auxiliary leaf chamber is left under dynamic.Therefore, the first nylon wire and the second nylon wire selection 80-120 mesh white Nylon net 1 are made.Auxiliary
Leaf chamber main body is black, high-elastic, 60 degree of hardness EVA foam elastomer block 2 (rectangular-shape, interior hollow section, that is, inner cavity), the
Two nylon wires are adhesively fixed with EVA foam elastomer block 2 with double-sided adhesive, formed inner cavity bottom end netted end face, can be used for place compared with
Small (short) silique, two-sided reinforcing surrounding above and below the first nylon wire plastic adhesive tape, intermediate unguyed area be 20mm ×
30mm, reinforce surrounding purpose be in order to enhance the first nylon wire intensity, and prevent the first nylon wire deform.It is put into sample auxiliary
It helps behind the inner cavity of leaf chamber and the first nylon wire is covered on inner cavity top opening (intermediate unguyed part and inner cavity top opening phase
It is right), and be fixed together with EVA foam elastomer block 2.To guarantee to assist leaf chamber that integrally there is certain elastic deformation amount, just
In the airflow channel that formation is closed.
There is certain thickness and longer silique, EVA foam elastomer block 2 for convenience of fixation (and guaranteeing airflow channel sealing)
Top lumen openings edge machined two groups of cracks (for example, every group three, be spaced uniform and arranged in parallel) along its length, and two
Group crack is symmetrical, constitutes leaf chamber slot 3 two-by-two, can be used for being embedded in, fixes silique.
Referring to Fig. 3, leaf chamber 6 is assisted to match with photosynthesis measurement system prophyll room, in prophyll room, upper leaf chamber 5 has
LED light source 9, lower leaf chamber 8 are connect with infrared analyzer 10, and host 11 is connect with infrared analyzer 10 by cable, and cable includes
Electric signal, electric power connection line and tracheae.Assist leaf chamber 6 the case where retaining the performances such as prophyll chamber shape and aeration, translucency
Under, guarantee that measured object is in the part that leaf chamber interior air-flow passes through always in the detection.It is fixed for convenience of the auxiliary installation of leaf chamber 6,
In square hole of 2 intracavity bottom of the EVA foam elastomer block opening processing as card slot 4,4 shape of card slot is agreed with lower leaf chamber gasket 7,
It is fixed in prophyll room by card slot 4.
When detecting siliqua of oilseed rape photosynthesis:
1) prepare to meet prophyll room (6400-02 or -02B the LED light source leaf chamber of the photosynthetic instrument of LI-6400) shape ruler first
Very little auxiliary leaf chamber 6.
2) if Pod length to be determined is less than 30mm, laid parallel is in auxiliary 6 the second nylon of intracavity bottom of leaf chamber
On the net；If length is more than or equal to 30mm, silique is sandwiched in parallel in leaf chamber slot 3.
3) the first nylon wire of auxiliary leaf chamber 6 is covered, then entire auxiliary leaf chamber 6 is fixed in lower leaf chamber 8, in closing
Leaf chamber 5 and lower leaf chamber 8.Carry out photosynthesis measurement；The other operations of photosynthesis measurement are the same as the photosynthetic instrument basic operation of LI-6400.
4) the photosynthetic data surveyed with LI6400FileEx software export host 11.
5) it is tested the measurement of silique total projection area
It is taken pictures, is write down photo resolution (dip) with camera, the sample number of photo mark and sample sample phase in photosynthetic instrument
Together, root measurement analysis software processing photo is washed with WinRHIZO, finds out the siliqua of oilseed rape total projection face placed in auxiliary leaf chamber
Product, unit conversion is at cm2.Or use WinDENDROTMThe silique total projection face placed in root system scanning system measurement auxiliary leaf chamber
Product, unit conversion is at cm2。
6) with silique total projection surface area substitute derived from leaf area in photosynthetic data, it is soft using LI6400FileEx
Part calculates photosynthetic parameters automatically.Such as: photosynthetic rate (Photo), stomatal conductance (Cond), intercellular CO2Concentration (Ci), transpiration rate
(Trmmol) physical signs such as.
Example 1, measured length are less than the siliqua of oilseed rape of 3cm
1) siliqua of oilseed rape is cut, laid parallel can be paved on the second nylon wire of intracavity bottom of auxiliary leaf chamber 6.
2) the vertical view picture that auxiliary 6 inner cavity of leaf chamber is taken with camera, writes down photo resolution (dip), picture makes marks
(consistent with photosynthetic instrument sample number).
3) entire auxiliary leaf chamber 6 is installed on the 6400- of the photosynthetic instrument of LI-6400 by the first nylon wire for covering auxiliary leaf chamber 6
In 02 or -02B LED light source leaf chamber, leaf chamber is closed, carries out photosynthesis measurement, is operated with photosynthetic instrument basic operation.
4) the photosynthetic data surveyed are exported with LI6400FileEx software.
5) photo of camera shooting is washed into root measurement analysis software with WinRHIZO and finds out siliqua of oilseed rape total projection area,
Unit conversion is at (cm2)。
6) with the silique total projection area substitute derived from leaf area in photosynthetic data, software is calculated automatically from light
Close parameter.Measured data is as shown in table 1:
1. siliqua of oilseed rape photosynthesis measurement result (< 3cm) of table
The siliqua of oilseed rape of example 2, measured length 3cm-10cm
1) siliqua of oilseed rape is cut, in leaf chamber slot 3, silique can be loaded onto each leaf chamber slot 3.
2) the vertical view picture that auxiliary 6 inner cavity of leaf chamber is taken with camera, writes down photo resolution (dip), picture makes marks
(consistent with photosynthetic instrument sample number).
3) entire auxiliary leaf chamber 6 is sandwiched in the 6400-02 of the photosynthetic instrument of LI-6400 by the first nylon wire for covering auxiliary leaf chamber 6
Or in -02B LED light source leaf chamber, leaf chamber is closed, opens light source, luminous intensity is set, carries out photosynthesis measurement, is operated with photosynthetic
Instrument basic operation.
4) the photosynthetic data surveyed are exported with LI6400FileEx software.
5) photo of camera shooting is washed into root measurement analysis software with WinRHIZO and finds out siliqua of oilseed rape total projection area,
Unit conversion is at (cm2)。
6) with the silique total projection area substitute derived from leaf area in photosynthetic data, software is calculated automatically from light
Close parameter.Measured data is as shown in table 2:
2. siliqua of oilseed rape photosynthesis measurement result (3-10cm) of table
Advantages of the present invention is as follows:
1) in the case where not changing instrument for determining photosynthesis device overall structure, accessory, connection, measurement performance, pass through increasing
Add auxiliary leaf chamber to expand the use scope of instrument, the photosynthesis of the silique of rape different times can be measured.
2) production of auxiliary leaf chamber is simple, low-cost, easy to use, reliable performance.
3) the photosynthetic purpose of measurement different times siliqua of oilseed rape has not been only reached, moreover, passing through increased auxiliary lobe
Room can also measure the photosynthesis of other class plants.Such as: rape, sesame, silk tree, beans pod class seed；The wheat of wheat
Awns, rice the crop and fruits such as spike of rice.
4) application range of LI-6400 (XT) is expanded, method is easy to operate, and it is easy to use, it is widely used, measurement is accurate
Property it is high.
5) auxiliary leaf chamber may be reused.
Claims (10)
1. a kind of auxiliary leaf chamber, it is characterised in that: including elastic material block, the upper surface of the elastic material block is provided with
Leaf chamber slot (3) and the first reticular structure layer, it is intracorporal in elastic material block that the lower surface of elastic material block is provided with indent
Card slot (4), the top surface of card slot (4) is provided with the second reticular structure layer, between the first reticular structure layer and the second reticular structure layer
It is provided with through the inner cavity of elastic material block；Leaf chamber slot (3) is including symmetry arrangement two-by-two in elastic material block upper surface
The crack of chamber opening both sides of the edge.
2. a kind of auxiliary leaf chamber according to claim 1, it is characterised in that: the elastic material block uses EVA foam system
At.
3. a kind of auxiliary leaf chamber according to claim 1, it is characterised in that: the first reticular structure layer and the second netted knot
Structure layer is made of nylon wire (1).
4. a kind of auxiliary leaf chamber according to claim 3, it is characterised in that: the nylon wire (1) is 80-120 mesh.
5. a kind of auxiliary leaf chamber according to claim 1, it is characterised in that: the first reticular structure layer and the second netted knot
Structure layer is white, and elastic material block is black.
6. a kind of photosynthesis measurement system, it is characterised in that: including photosynthesis measurement system leaf chamber and auxiliary leaf chamber
(6), the auxiliary leaf chamber (6) includes elastic material block, the upper surface of the elastic material block be provided with leaf chamber slot (3) with
And the first reticular structure layer, the lower surface of elastic material block is provided with indent in the intracorporal card slot of elastic material block (4), card slot
(4) top surface is provided with the second reticular structure layer, is provided between the first reticular structure layer and the second reticular structure layer through bullet
The inner cavity of property material block；Leaf chamber slot (3) is including symmetry arrangement two-by-two in elastic material block upper surface lumen openings two sides
The crack of edge；Auxiliary leaf chamber (6) is fixed on the indoor gasket of photosynthesis measurement system leaf (7) by card slot (4), is sealed
The hollow space for padding (7) is opposite with inner cavity.
7. a kind of method of measurement different times vegetable seeds, fruit photosynthesis, it is characterised in that: the following steps are included:
1) block is processed using elastic material；Run through the inner cavity of block upper and lower surfaces in bulk inner processing；On block
The both sides of the edge of surface lumen openings process the crack of symmetry arrangement two-by-two；Processing at the lumen openings of block lower surface can cover
The counterbore of the open edge；The first reticular structure and the second reticular structure are processed, the second reticular structure is fixed in counterbore, and
Covering blocks lower surface lumen openings；
2) vegetable seeds or fruit are put into inner cavity and are laid on the second reticular structure, or by vegetable seeds or fruit both ends
It is embedded in and is fixed in crack, so that vegetable seeds or fruit are across block upper surface lumen openings；Then by the first netted knot
Structure is covered and is fixed at the lumen openings of block upper surface；
3) after step 2), using counterbore as card slot (4), block is fixed on photosynthesis measurement system leaf chamber by counterbore
On interior gasket (7), keep the hollow space of gasket (7) opposite with inner cavity；
4) photosynthesis measurement system leaf chamber is closed, block is made to form closed airflow channel at inner cavity by being extruded in；
5) LED light source (9) for opening photosynthesis measurement system leaf chamber, automatically obtains plant species using photosynthesis measurement system
The photosynthetic data of son or fruit；
6) projected area of leaf area vegetable seeds or the tested part of fruit in photosynthetic data is replaced, thus to photosynthetic number
According to being modified；
7) Physiological Index of Photosynthesis of vegetable seeds or fruit is calculated according to revised photosynthetic data.
8. a kind of method of measurement different times vegetable seeds, fruit photosynthesis, feature exist according to claim 7
In: the acquisition methods of the projected area of the vegetable seeds or the tested part of fruit are as follows: measure plant using root system scanning system
Seed or fruit are located at the total projection area of inner cavity point, alternatively, acquisition inner cavity image, analyzes software meter using root measurement is washed
Calculate the total projection area of vegetable seeds or fruit in the image.
9. a kind of method of measurement different times vegetable seeds, fruit photosynthesis, feature exist according to claim 7
In: the physical signs includes photosynthetic rate, stomatal conductance, intercellular CO2Concentration and transpiration rate.
10. a kind of method of measurement different times vegetable seeds, fruit photosynthesis, feature exist according to claim 7
In: the elastic material is selected from EVA foam, and the first reticular structure and the second reticular structure are selected from nylon wire.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810778285.XA CN109142218A (en) | 2018-07-16 | 2018-07-16 | A method of utilizing photosynthesis measurement system measurement different times vegetable seeds, fruit photosynthesis |
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| CN201810778285.XA CN109142218A (en) | 2018-07-16 | 2018-07-16 | A method of utilizing photosynthesis measurement system measurement different times vegetable seeds, fruit photosynthesis |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111398285A (en) * | 2020-05-14 | 2020-07-10 | 上海乾菲诺农业科技有限公司 | Photosynthetic apparatus capable of fixing blade position |
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Application publication date: 20190104 |