CN207062248U - A kind of culture box using gaseous mixture culture anaerobe - Google Patents
A kind of culture box using gaseous mixture culture anaerobe Download PDFInfo
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- CN207062248U CN207062248U CN201720294014.8U CN201720294014U CN207062248U CN 207062248 U CN207062248 U CN 207062248U CN 201720294014 U CN201720294014 U CN 201720294014U CN 207062248 U CN207062248 U CN 207062248U
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Abstract
It the utility model is related to a kind of culture box using gaseous mixture culture anaerobe.Specifically, culture box of the present utility model includes box body and top cover of faceplate；Wherein, the tray interior is divided into the cultivation region on top and the exclusion region of bottom, and the cultivation region and exclusion region separate via supportive grid；Air inlet and gas outlet are provided with the top cover of faceplate.The utility model aim to provide it is a kind of cheap, it is easy to operate, the culture box that can be cultivated in standard incubator, Anaerobic culturel purpose is used the syngas as to reach.
Description
Technical field
It the utility model is related to a kind of culture box using gaseous mixture culture anaerobe.
Background technology
Late 1980s, scientific research personnel successively chicken manure, seawater deposit and be to have etc. to be found that in material
Some can utilize the strain of synthesis gas producing and ethanol, find after this and successively that other can use the syngas as sole carbon source
Strain, hereafter just started the research of Synthesis gas zymotechnique, but because such strain is mainly anaerobic bacteria, for cultivating
Oxygen in journey is easily sensitive.
The culture of the current this anaerobe using synthesis gas typically uses anaerobic culture box, and this incubator needs
Substantial amounts of high pure nitrogen or carbon dioxide are consumed to drive away the air in case, is then passed through mixed gas again.This device is in itself
It is expensive, and need to consume a large amount of gases.
The content of the invention
The utility model provides a kind of culture box for gaseous mixture culture anaerobe.
Specifically, culture box of the present utility model includes：
Box body；With
Top cover of faceplate；
Wherein, the tray interior is divided into the cultivation region on top and the exclusion region of cassette bottom, the cultivation region and exclusion region warp
Separated by supportive grid；Air inlet and gas outlet are provided with the top cover of faceplate.
In one or more embodiments, via screw thread or flange seal between the top cover of faceplate and the box body.
In one or more embodiments, the cut-off is distinguished into 2~8 isolation blocks.
In one or more embodiments, each isolation block is formed by dividing plate, or independent open containers are placed in box
Body bottom and form each isolation block.
In one or more embodiments, the dividing plate is dismountable dividing plate, or the dividing plate and the box body one
It is body formed.
In one or more embodiments, the exclusion region is highly the 1/4~1/3 of box body height.
In one or more embodiments, air inlet pipe and escape pipe are provided with the top cover of faceplate, respectively via institute
State air inlet and gas outlet connects with box body.
In one or more embodiments, pressure detecting mouth is additionally provided with the top cover of faceplate, the culture box is also
Including pressure gauge, the pressure gauge connects via the pressure detecting mouth with box body.
In one or more embodiments, valve and optional micropore are respectively arranged with the air inlet pipe and escape pipe
Filter membrane.
In one or more embodiments, the box body material is transparent material.
In one or more embodiments, the box body material is glass, makrolon or polymethyl methacrylate.
In one or more embodiments, the box body is highly 10cm~50cm, and length is 15cm~60cm, width
For 15~40cm.
In one or more embodiments, the top cover of faceplate thickness is 5mm~15mm.
In one or more embodiments, box body and top cover of faceplate are sealed into flange seal, flange and top cover of faceplate
Between be provided with sealing ring.
In one or more embodiments, the top cover of faceplate edge is provided with 2~10 flange nut bolt connections.
In one or more embodiments, the caliber of the air inlet pipe and escape pipe is each independently 4 × 2.5mm, 6
× 4mm, 8 × 5mm, 10 × 6.5mm or 16 × 12mm.
In one or more embodiments, the valve is needle-valve, stop valve, ball valve, butterfly valve or plug valve.
In one or more embodiments, the air inlet pipe is not extended into box body；The escape pipe goes out described in
Gas port, it is inserted into cultivation region.
In one or more embodiments, the micro porous filtration membrane aperture is 0.22um~0.45um.
In one or more embodiments, culture box of the present utility model includes：
Box body；With
It is connected with the box body to form the top cover of faceplate of sealing space in box body；
Wherein, the tray interior is divided into the cultivation region on top and the exclusion region of bottom, the cultivation region and exclusion region warp
Separated by supportive grid；
The culture box also includes air inlet, gas outlet and the pressure detecting mouth being arranged on top cover of faceplate, and passes through respectively
Air inlet pipe, escape pipe and the pressure gauge connected by the air inlet, gas outlet and pressure detecting mouth with the box body.
In one or more embodiments, the culture box also includes being arranged on valve in the air inlet pipe and optionally
Micropore filtering film, and the valve and micropore filtering film being arranged on the escape pipe.
In one or more embodiments, the micropore filtering film is arranged between valve and air inlet or gas outlet.
Other aspects of the present utility model are aobvious and easy to those skilled in the art due to this disclosure
See.
Brief description of the drawings
Fig. 1：The utility model synthesis gas culture anaerobe cultivates the structure chart of box, wherein：1, box body；2, upper capping
Plate；3, sealing ring；4, air inlet pipe；5, pressure gauge；6, escape pipe；7, valve；8, micropore filtering film；9, valve；10, micro porous filtration
Film；11, support grid；12, bottom exclusion region；13, flange；14, cultivation region.
Embodiment
The utility model aims to provide a kind of cheap, easy to operate, culture that can be cultivated in standard incubator
Box, Anaerobic culturel purpose is used the syngas as to reach.By using provided herein is culture box realize this paper each side appearance
's.
Culture box of the present utility model includes box body and top cover of faceplate.
The material of box body can be transparent material, including but not limited to glass, makrolon, polymethyl methacrylate
Deng.Generally, the height of box body can be in the range of 10cm~50cm, and length can be in the range of 15cm~60cm, width
Can be in the range of 15~40cm.Box body can have various suitable shapes, such as cylinder, square, cuboid
Deng.
Two regions can be set in tray interior, and top is cultivation region, for placing culture vessel, such as culture dish；Bottom, i.e.,
Box body bottom is exclusion region, for placing some functional materials, such as Anaerobic indicator, oxygen scavenger, drier etc..
Exclusion region can be formed by separating with the dividing plate that box body is integrally formed, and generally be provided with 2~8 in the horizontal direction
Isolation block.Or in certain embodiments, dividing plate is dismountable dividing plate.In this case, can be in box body bottom not
With fixing groove is set on position, for blocking dividing plate.Multiple fixing grooves can be set, set different in order to according to different needs
The isolation block of size.Or in certain embodiments, the removable of independent upper opening can be placed in box body bottom
Container, such as 2~8 removable containers, box body bottom is placed side by side, and by placing support lattice on the top of these containers
Grid, so as to which tray interior is divided into cultivation region and exclusion region.
Supportive grid can be any suitable material, including but not limited to stainless steel, glass or lucite.Supportive grid
Can directly frame on dividing plate or open containers.Or in certain embodiments, the bottom on the inside of box body sets inwardly projecting
Part, to support the supportive grid.The inwardly projecting part can be the part protruded in several positions, can also
It is the projection for being linked to be ring-type, the supportive grid is supported as long as can stablize.For example, the bottom on the inside of box body is set
The part of 2~6 protrusions, for supporting the supportive grid.Preferably, the part of these protrusions is symmetrical.It should be understood that
, through hole is provided with supportive grid, to allow the gas of cultivation region and exclusion region to circulate.In addition, the height of exclusion region is led to
The 1/4~1/3 of Chang Kewei box body height.
Top cover of faceplate is generally connected with the box body, forms confined space in tray interior, the air exclusion with outside.It is logical
Often, the sealing of top cover of faceplate and box body can be thread seal or flange seal.
During using flange seal, culture box of the present utility model is additionally provided with flange, and top cover of faceplate is via flange and box body
It is tightly connected.Sealing ring can be also further provided between top cover of faceplate and flange.The material of sealing ring can be this area
Conventional material, such as can be rubber or polytetrafluoroethylene (PTFE).
In addition, 2~10 flange nut bolt connections also can be set in the edge of top cover of faceplate.Generally, it is set
Flange nut, which is bolted on top cover of faceplate, is in symmetrical position.
The thickness of top cover of faceplate is usually 5mm~15mm.
Air inlet, gas outlet and pressure detecting mouth are may be provided with top cover of faceplate.Air inlet pipe, outlet are can be inserted into air inlet
Escape pipe is can be inserted into mouthful, pressure detecting mouth then can be used for connection pressure gauge and tray interior.Generally, air inlet pipe does not extend through
Top cover of faceplate is with into tray interior, and gas outlet can extend through top cover of faceplate, and is inserted into the cultivation region of tray interior.
Air inlet pipe and the material of escape pipe can be the conventional materials in this area, and caliber can be caliber specification generally in the art, example
Such as 4 × 2.5mm or 6 × 4mm or 8 × 5mm or 10 × 6.5mm or 16 × 12mm.
Valve is may be provided with air inlet pipe and escape pipe.Valve can be valve commonly used in the art, include but is not limited to
Needle-valve, stop valve, ball valve, butterfly valve or plug valve.Optionally, micropore filtering film is provided between valve and air inlet, is being gone out
Micropore filtering film is provided between gas port and valve.Micropore filtering film commonly used in the art can be used, its usual aperture exists
In the range of 0.22um~0.45um.
Fig. 1 shows the structural representation of one culture box example of the utility model.The culture box include box body 1 and with
Box body 1 coordinates the top cover of faceplate 2 of snapping.Sealing ring 3 is provided with the border edge that box body 1 is open, top cover of faceplate 2 is pressed in
On sealing ring 3, and it is fastened on via flange 13 on box body 1.Six flange nut bolts are provided with the edge of top cover of faceplate 2 to connect
Connect, for tightening panel 2 and box body 1, be allowed to seal.Top cover of faceplate 2 is provided with air inlet pipe 4, pressure gauge 5 and escape pipe 6.Enter
Tracheae 4 is provided with valve 7 and micropore filtering film 8.Escape pipe 6 is provided with valve 9 and micropore filtering film 10.Upper strata is culture in box body 1
Area 14, lower floor are exclusion regions 12, are separated between cultivation region and bottom cut-off by support grid 11.
When carrying out microculture using culture box of the present utility model, the leak detection of equipment is carried out first.Stop leakage in the roof and finish
Afterwards, functional reagent, including oxygen scavenger, Anaerobic indicator and optional drier are put into exclusion region.In some embodiments
In, to be uniformly distributed these functional reagents, it can be spaced in separating element and be put into the functional reagent.Oxygen scavenger, anaerobism refer to
Show that agent and drier can be oxygen scavenger well known in the art, Anaerobic indicator and drier.For example, oxygen scavenger can be palladium,
Anaerobic indicator can be methylene blue reagent, and drier can be alundum (Al2O3).As it was noted above, in the dismountable situation of dividing plate
Under, dividing plate can be first inserted as needed, forms each separating element, be then sequentially placed into each functional reagent again.Using independent
In the case of the container of upper opening, each container can be first placed, then adds corresponding functional reagent in individual container successively；Or
After each functional reagent being added into each container, then each container is sequentially placed in box body bottom.It is afterwards that supportive grid is horizontal
It is placed on the projection set by the dividing plate, container or box body bottom.
Then, the culture dish after inoculation is positioned in the supportive grid of tray interior；Multiple culture dishes can be stacked on
In box body cultivation region.Then top cover of faceplate is covered, confined space is formed in tray interior.For example, in the feelings using thread seal
Under condition, top cover of faceplate is screwed；In the case of using flange seal, flange nut bolt connection is tightened.
The valve of air inlet pipe is opened, forming gas is passed through to air inlet pipe, gas is entered tray interior.Then outlet is opened
The valve of pipe, be passed through forming gas is utilized to catch up with the oxygen of most tray interior, until Anaerobic indicator is shown as in exclusion region
It is colourless.It is then shut off the valve of escape pipe.When the pressure of tray interior is in 0.01~0.05MPa, the valve of air inlet pipe is closed
Door.Afterwards, the culture box can be placed in and is most suitable for being cultivated in the incubator of objective microbe growth temperature.
Culture box of the present utility model is applied to the various various microorganisms of forming gas culture.For example, forming gas can be with
It is H2With CO and CO2At least one of mixture.
The utility model has advantages below：
1st, box body appearance transparent is cultivated, the upgrowth situation of strain can be observed at any time；
2nd, enter escape pipe and connect miillpore filter, prevent gas from bringing miscellaneous bacteria into；
3rd, the mode being combined using culture gas is passed through with oxygen scavenger, the culture environment of anaerobism is ensured；
4th, for using the syngas as the microculture of sole carbon source, it is ensured that the continuation in cultivation cycle
Ventilation；
5th, lid installation pressure gauge can allow in box the state for keeping pressure-fired, prevent oxygen from penetrating into；With
6th, can be cultivated compared to the tradition culture box for relying on anaerobic system deoxygenation, the utility model after independent deoxygenation.
Hereafter the utility model will be illustrated in a manner of specific embodiment.It should be understood that these embodiments are only illustrative
, not limit the utility model.Unless otherwise noted, occur in this specification, claims and specific implementation case
All numerals, such as pressure numerical value should not be construed absolute exact value in gas componant, box, and the numerical value is in this area
What interior those of ordinary skill was understood, the error range that known technology is allowed.
It should be understood that in the scope of the utility model, above-mentioned each technical characteristic of the present utility model and below (such as implementation
Example) in specifically describe each technical characteristic between can be combined with each other, so as to form each preferable technical side of the utility model
Case.
Embodiment
Culture box is prepared as shown in Figure 1, and the culture medium includes box body 1 and coordinates the top cover of faceplate 2 of snapping with box body 1.
Sealing ring 3 is provided with the border edge that box body 1 is open, top cover of faceplate 2 is pressed on sealing ring 3, and is fastened via flange 13
On box body 1.The edge of top cover of faceplate 2 is provided with six flange nut bolt connections, for tightening panel 2 and box body 1, is allowed to
Sealing.Top cover of faceplate 2 is provided with air inlet pipe 4, pressure gauge 5 and escape pipe 6.Air inlet pipe 4 is provided with valve 7 and micropore filtering film 8.Go out
Tracheae 6 is provided with valve 9 and micropore filtering film 10.Upper strata is cultivation region 14 in box body 1, and lower floor is exclusion region 12, cultivation region with
Separated between the cut-off of bottom by support grid 11.
When carrying out microculture using the culture box, the leak detection of equipment is carried out first, and leak detection finishes, into bottom cut-off 12
It is put into oxygen scavenger palladium, Anaerobic indicator methylene blue reagent, drier alundum (Al2O3).Then supportive grid 11 is lain in a horizontal plane in into bottom
On cut-off 12.
Culture dish 14 after inoculation is positioned in the supportive grid 11 in box body 1；After top cover of faceplate 2 is covered, use
Flange nut bolt tightens upper and lower cover plates.Valve 7 is opened, forming gas is accessed from air inlet pipe 4, gas is entered in box.Then
Valve 9 is opened, gas needed for Spawn incubation is caught up with oxygen in most culture box, until anaerobism instruction is shown as nothing in bottom cut-off 12
Color.Valve 9 is closed, makes pressure gauge registration in 0.05MPa, closes valve 7, closes gas cylinder.
Seal box is positioned over and is most suitable for being cultivated in the incubator of objective microbe growth temperature.
The utility model is exemplarily described above in conjunction with accompanying drawing, it is clear that the utility model specific implementation not by
The limitation of aforesaid way, if the various improvement that methodology and technical scheme of the present utility model are carried out are employed, or without
Improvement directly applies to other occasions, within the scope of protection of the utility model.
Claims (9)
1. one kind culture box, it is characterised in that the culture box includes：
Box body；With
Top cover of faceplate；
Wherein, the tray interior is divided into the cultivation region on top and the exclusion region of bottom, and the cultivation region and exclusion region are via branch
Grid is supportted to separate, wherein, the exclusion region has 2~8 isolation blocks, and each isolation block is formed by dividing plate, or by can be removed
Open containers be placed in box body bottom and formed；The dividing plate is dismountable dividing plate, or the dividing plate and box body one
Shaping；Highly it is the 1/4~1/3 of box body height with the exclusion region；With
Air inlet and gas outlet are provided with the top cover of faceplate.
2. culture box as claimed in claim 1, it is characterised in that
Thread seal is sealed between the top cover of faceplate and the box body；Or
Flange seal is sealed between the top cover of faceplate and the box body, wherein, set between flange and the top cover of faceplate
There is sealing ring, the top cover of faceplate edge is provided with 2~10 flange nut bolt connections.
3. culture box as claimed in claim 1, it is characterised in that air inlet pipe and escape pipe are provided with the top cover of faceplate,
Connected respectively via the air inlet and gas outlet with box body；The air inlet pipe is not extended into box body；The escape pipe passes through
The gas outlet, is inserted into cultivation region.
4. the culture box as any one of claim 1-2, it is characterised in that the top cover of faceplate has following any
Individual or whole two features：
(a) the top cover of faceplate thickness is 5mm~15mm；With
(b) pressure detecting mouth is additionally provided with the top cover of faceplate, the culture also included pressure gauge of box is via the pressure
Detection mouth connects with box body.
5. as claimed in claim 3 culture box, it is characterised in that be respectively arranged with the air inlet pipe and escape pipe valve and
Optional micropore filtering film.
6. culture box as claimed in claim 5, it is characterised in that the culture box has following one or more features：
(i) caliber of the air inlet pipe and escape pipe is each independently 4 × 2.5mm, 6 × 4mm, 8 × 5mm, 10 × 6.5mm or 16
×12mm；
(ii) valve is needle-valve, stop valve, ball valve, butterfly valve or plug valve；With
(iii) the micro porous filtration membrane aperture is 0.22um~0.45um.
7. culture box as claimed in claim 1, it is characterised in that the box body is highly 10cm~50cm, length 15cm
~60cm, width are 15~40cm.
8. culture box as claimed in claim 1, it is characterised in that
The top cover of faceplate is connected with the box body to form sealing space in box body；
The culture box also includes the pressure detecting mouth being arranged on top cover of faceplate, and respectively via the air inlet, gas outlet
Air inlet pipe, escape pipe and the pressure gauge connected with pressure detecting mouth with the box body；With
In the exclusion region, each isolation block is formed by dividing plate；Wherein, the dividing plate is dismountable dividing plate, or the dividing plate
It is integrally formed with the box body.
9. culture box as claimed in claim 8, it is characterised in that the culture box also includes being arranged in the air inlet pipe
Valve and optional micropore filtering film, and the valve and micropore filtering film being arranged on the escape pipe；The air inlet pipe is not
Extend into box body；The escape pipe passes through the gas outlet, is inserted into the cultivation region.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720294014.8U CN207062248U (en) | 2017-03-24 | 2017-03-24 | A kind of culture box using gaseous mixture culture anaerobe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720294014.8U CN207062248U (en) | 2017-03-24 | 2017-03-24 | A kind of culture box using gaseous mixture culture anaerobe |
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| Publication Number | Publication Date |
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| CN207062248U true CN207062248U (en) | 2018-03-02 |
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| CN201720294014.8U Active CN207062248U (en) | 2017-03-24 | 2017-03-24 | A kind of culture box using gaseous mixture culture anaerobe |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114106986A (en) * | 2021-12-23 | 2022-03-01 | 梁华全 | Probiotic culture equipment |
-
2017
- 2017-03-24 CN CN201720294014.8U patent/CN207062248U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114106986A (en) * | 2021-12-23 | 2022-03-01 | 梁华全 | Probiotic culture equipment |
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Effective date of registration: 20220105 Address after: Room 120, floor 1, building 10 [8-5], courtyard 4, Sanjianfang Nanli, Chaoyang District, Beijing 100024 Patentee after: Ji state Laibo (Beijing) Biotechnology Development Co.,Ltd. Address before: 200433 room 901-55, No. 127, Guotong Road, Yangpu District, Shanghai Patentee before: SHANGHAI JITAILAI BIOTECHNOLOGY CO.,LTD. |