CN114106986A - Probiotic culture equipment - Google Patents
Probiotic culture equipment Download PDFInfo
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- CN114106986A CN114106986A CN202111591048.0A CN202111591048A CN114106986A CN 114106986 A CN114106986 A CN 114106986A CN 202111591048 A CN202111591048 A CN 202111591048A CN 114106986 A CN114106986 A CN 114106986A
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- cavity
- side wall
- shell
- fixedly arranged
- incubator
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- 239000006041 probiotic Substances 0.000 title claims abstract description 54
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 54
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 39
- 238000007789 sealing Methods 0.000 claims abstract description 52
- 238000001514 detection method Methods 0.000 claims abstract description 26
- 238000003756 stirring Methods 0.000 claims description 29
- 230000005540 biological transmission Effects 0.000 claims description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 18
- 230000001580 bacterial effect Effects 0.000 claims description 10
- 230000008569 process Effects 0.000 claims description 9
- 239000012528 membrane Substances 0.000 claims description 7
- 238000011084 recovery Methods 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 230000003019 stabilising effect Effects 0.000 claims 1
- 239000001963 growth medium Substances 0.000 abstract description 13
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 8
- 239000001301 oxygen Substances 0.000 abstract description 8
- 229910052760 oxygen Inorganic materials 0.000 abstract description 8
- 230000008859 change Effects 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 13
- 241000233866 Fungi Species 0.000 description 9
- 239000007789 gas Substances 0.000 description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 230000008901 benefit Effects 0.000 description 6
- 238000012546 transfer Methods 0.000 description 6
- 230000007306 turnover Effects 0.000 description 6
- 238000012258 culturing Methods 0.000 description 5
- 102000038379 digestive enzymes Human genes 0.000 description 4
- 108091007734 digestive enzymes Proteins 0.000 description 4
- 229910001873 dinitrogen Inorganic materials 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 230000004099 anaerobic respiration Effects 0.000 description 3
- 210000001035 gastrointestinal tract Anatomy 0.000 description 3
- 238000012423 maintenance Methods 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 230000009699 differential effect Effects 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000012466 permeate Substances 0.000 description 2
- 210000000813 small intestine Anatomy 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 230000000087 stabilizing effect Effects 0.000 description 2
- 102000006395 Globulins Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- MSFSPUZXLOGKHJ-UHFFFAOYSA-N Muraminsaeure Natural products OC(=O)C(C)OC1C(N)C(O)OC(CO)C1O MSFSPUZXLOGKHJ-UHFFFAOYSA-N 0.000 description 1
- 108010013639 Peptidoglycan Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000003305 autocrine Effects 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 210000004443 dendritic cell Anatomy 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000005934 immune activation Effects 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000822 natural killer cell Anatomy 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000008621 organismal health Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
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- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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Abstract
The invention belongs to the field of probiotic culture equipment, and relates to probiotic culture equipment, which comprises a shell and is characterized in that a sealing plate is arranged on the shell, a sealing device is arranged on the sealing plate and is used for isolating the inside of a cavity from the outside to ensure the airtightness of the inside of the cavity, a collecting cavity is fixedly arranged on the lower side wall of the shell, a tilting device is arranged on the collecting cavity and is used for shaking the cavity inside the cavity, and the equipment is a probiotic incubator capable of automatically controlling the sealing condition of the cavity, can monitor the inside of the cavity while sealing the inside of the cavity, dynamically balance and adjust the internal change of the cavity, and ensures that a good propagation environment is provided for probiotics. The culture medium cavity can be internally provided with a timing tilting device, and the culture medium is internally stirred, so that the colony detection equipment is provided with a switch to prevent excessive oxygen from entering the cavity.
Description
Technical Field
The invention belongs to the field of probiotic culture equipment, and particularly relates to probiotic culture equipment.
Background
It is known that probiotics can synthesize digestive enzymes, and together with digestive enzymes synthesized by animals, the digestive enzymes participate in digestion of nutrients in intestinal tracts, stimulate the animals to secrete the digestive enzymes, reduce the crypt depth of small intestines, increase the villus height, increase the surface area of the small intestines and promote absorption of nutrients in the intestinal tracts. The self structure of the probiotics, such as components of peptidoglycan, lipoteichoic acid and the like, can be used as an antigen to directly play a role in immune activation, or can stimulate a host immune system through an autocrine immune activator, so that the immunity of animals is improved, the activity of innate immune cells and natural killer cells of an organism is enhanced, dendritic cells are activated, the organism is stimulated to generate cell factors, B cells are stimulated to secrete antibodies, and the probiotics can stimulate intestinal tracts to generate secretory globulin A and protect the health of the organism.
The probiotics is beneficial to body health and has good development prospect in the future.
This equipment design can independently control the probiotic incubator of cavity sealing condition, can seal up cavity inside and carry out the inside control to the cavity, carries out dynamic balance adjustment to the internal change of cavity, guarantees to provide good reproduction environment for the probiotic.
The timing tilting device can be designed inside the culture medium cavity, and the culture medium is stirred, so that probiotics can grow more easily.
And be equipped with the equipment that detects the bacterial colony at any time at the equipment side, be equipped with on it to the airtight switch of cavity, prevent when the sample, inside too much oxygen enters into the cavity, keep off oxygen for anaerobism probiotic.
Disclosure of Invention
The invention aims to solve the problems in the prior art and provides the probiotic culture equipment, which can dynamically balance and adjust internal parameters when a cavity is sealed, so as to provide a good environment for propagation of probiotics.
The purpose of the invention can be realized by the following technical scheme: the utility model provides a benefit fungus cultivation equipment, includes the casing, its characterized in that, be equipped with the closing plate on the casing, be equipped with sealing device on the closing plate, sealing device is used for guaranteeing the inside seal of cavity with outside isolated opening the cavity, the fixed collection chamber that is equipped with under the casing on the lateral wall, be equipped with tilting device on the collection chamber, tilting device is used for rocking the inside cavity of cavity, tilting device is including rotating collect the motor frame in the chamber, the motor frame internal fixation is equipped with the motor, the output of motor is equipped with the output shaft, be equipped with on the output shaft and turn over the device of stirring, turn over the device and be used for stirring turning over the nutrition base that the bacterium cultivateed, be equipped with detection device on the lateral wall about the casing, detection device is used for carrying out the detection to the inside bacterial colony of cavity.
Further, the sealing device comprises a thick sealing gasket fixed on the lower side wall of the sealing plate, a balancing device is arranged on the thick sealing gasket and used for adjusting dynamic balance of various parameters inside the cavity, locking nails are arranged on the sealing plate, clamping devices are arranged on the outer sides of the locking nails and used for guaranteeing stability of the sealing device and clamping the stabilizing device.
Further, the balancing device comprises a gas controller fixed on the sealing plate, a humidification cooler is fixedly arranged on the sealing plate, a pipeline is arranged at the lower end of the gas controller and the humidification cooler, the pipeline extends downwards and extends into the thick sealing gasket, a lead screw is fixedly arranged in the thick sealing gasket, an electric slider is arranged on the lead screw in a sliding mode, a detector is fixedly arranged in the inner portion of the lower side wall of the thick sealing gasket and used for detecting the air pressure, the temperature and the humidity in the cavity and transmitting electric signals to the electric slider to operate the electric slider to slide on the lead screw, an opening is formed in the electric slider, and when the opening coincides with the pipeline, the opening together with the cavity, the gas controller and the humidification cooler are arranged.
Furthermore, the clamping device comprises a clamping slide block which slides at the lower end of the sealing plate, the clamping slide block can slide into the side wall of the shell, a rotating push rod is arranged in the clamping slide block in a rotating mode, a bevel gear is arranged at the end, close to the axis, of the rotating push rod, a turning cavity is arranged at the end, close to the axis, of the clamping slide block, a bevel gear is arranged in the turning cavity in a rotating mode, a clamping block is arranged on the bevel gear in a rotating mode, the bevel gear can be in meshing transmission with the end, close to the axis, of the rotating push rod, when the bevel gear rotates, the clamping block can be driven to rotate, and the clamping block is ejected outwards or withdrawn.
Further, the tilting device comprises a tilting frame fixedly arranged on the collecting cavity, a sliding groove is formed in the tilting frame, an incubator is arranged on the tilting frame in a sliding mode and can swing back and forth on the tilting frame, a recovery device is arranged on the collecting cavity, and the recovery device is used for excessive moisture generated in the bacteria culturing process.
Further, recovery unit is including fixing the water collector on the collection chamber, the fixed membrane that permeates water that is equipped with on the lateral wall under the culture apparatus, unnecessary moisture can pass through the membrane that permeates water, process the water collector flows in collect intracavity portion.
Furthermore, the stirring device comprises a differential fixed on the output shaft, a shaft sleeve is fixedly arranged on the outer side of the differential, the differential can achieve the effect that the shaft sleeve and the output shaft have different rotating frequencies, a gear transmission box is fixedly arranged on the shaft sleeve, stirring blades are rotatably arranged on the outer side of the gear transmission box, and rotating force on the output shaft is transmitted to the stirring blades through the gear transmission box.
Further, detection device is including sliding be in the outer slide bar of detection on the lateral wall about the casing, it is equipped with the detection push rod to slide in the outer slide bar of detection, the fixed connecting plate that is equipped with on the inside wall about the casing, be equipped with transmission chamber piece in the connecting plate, transmission chamber piece internal rotation is equipped with the trailing wheel, the incubator internal rotation is equipped with the reel, it is equipped with the opening slide to slide in the incubator, the incubator internal fixation is equipped with the bearing, it is equipped with the diversion transfer line to rotate on the bearing, the opening slide with the reel with be connected with the rope between the diversion transfer line, the outer slide bar of detection can drive the trailing wheel rotates.
When using this equipment to carry out the cultivation of probiotic, put into the culture apparatus inside with the culture medium, through the closing plate with casing internal seal, and adjust inside air through the humidification cooler, take oxygen out and fill nitrogen gas into, and detect inside air pressure constantly, the temperature, humidity isoparametric, carry out the inside parameter to the useful department of cultivation probiotic of automatic adjustment casing, can slide to inside through detecting the outer slide bar, stretch into the culture apparatus inside with the detection push rod, carry out the inside probiotic detection of culture apparatus, guarantee that the probiotic of cultivateing reaches the standard, and at the stirring of the in-process of cultivateing the probiotic tilting and the inside stirring of culture apparatus, prevent inside culture medium center department high temperature scheduling problem, simultaneously will too much moisture filtering in stirring process, in order to avoid too much the reproduction that influences the probiotic.
When the sealing device is used, the rotating push rod is pushed, the clamping slide block is integrally pushed into the side wall of the shell, the rotating push rod rotates, the bevel gear is driven to rotate when the rotating push rod rotates, the clamping block can be popped out when the bevel gear rotates, the clamping block can clamp the clamping slide block in the shell, the clamping slide block can also clamp the locking nail, the locking nail is prevented from sliding up and down in the vertical direction, the effect that the shell is covered and sealed by the sealing plate is achieved, after the shell is sealed, the detector is started to dynamically monitor the interior of the cavity, when the temperature in the shell is overhigh, the electric slide block slides towards the right side, an opening in the electric slide block is communicated with a pipeline under a humidifying cooler to spray atomized water vapor for cooling, when the air pressure in the shell is unstable, the electric slide block towards the left side, the opening of the electric slide block is communicated with the pipeline under the air controller, and the air pressure in the shell is dynamically balanced through the air controller, prevent when detecting, nitrogen gas is outwards revealed, leads to outside oxygen to get into, can guarantee the inside sealed effect of device to the effect of the inside dynamic balance of maintenance device can provide better living reproduction environment for the probiotic.
Set for the fixed time interval and carry out the tilting to the incubator, the incubator rocks around on the frame that tilts and is favorable to unnecessary moisture to flow out downwards, flow into collection intracavity portion through the water collector, can produce anaerobic respiration and produce moisture at the probiotic reproduction in-process, need carry out the collection to this, and starter motor, opening sliding plate motor is driving the output shaft and is rotating, and the differential effect through differential mechanism reaches the effect with axle sleeve control rotational speed, make axle sleeve and output shaft slew velocity poor effect, and it is faster than the axle sleeve to be output shaft rotational efficiency, when the output shaft rotates, can carry out the transmission through gear drive case again, will drive and turn over the stirring leaf and rotate, it can carry out the stirring of the inside culture medium of incubator to turn over the stirring leaf and rotate, avoid the inside culture medium central temperature of incubator too high, lead to probiotic reproduction not good.
In the in-process of living fungus reproduction of benefit, carry out the detection to the bacterial colony, detect the whole inside slip of outer slide bar to the casing, the in-process that detects outer slide bar and slide in can drive the follower wheel and rotate, the follower wheel rotates and can drive the diversion transfer line and rotate, the diversion transfer line can carry out tightening up of rope, the rope is tightened up the back open slide and can be slided to keeping away from axle center end department, it can enter into the incubator inside to detect outer slide bar, it stretches into the incubator inside to detect the push rod afterwards, carry out the inside bacterial colony sampling of incubator, can guarantee at the in-process of cultivateing the benefit fungus, the fixed point is regularly surveyed the appearance and is detected, guarantee the quality and the quantity that the benefit fungus was cultivateed, avoid cultivateing the too much or too few condition of benefit fungus.
Drawings
Fig. 1 is an appearance structure schematic diagram of a probiotic culture device.
Fig. 2 is an internal cross-sectional view of a probiotic culturing apparatus.
Fig. 3 is a sectional view taken along a-a in fig. 2.
Fig. 4 is an enlarged view of the structure at B in fig. 2.
Fig. 5 is an enlarged view of the structure at C in fig. 3.
In the figure, 10, the housing; 11. a culture device; 12. a gas controller; 13. a humidifying and cooling device; 14. a pipeline; 15. a lead screw; 16. an electrical slider; 17. a detector; 18. a sealing plate; 19. locking nails; 20. a thick gasket; 21. clamping the sliding block; 22. rotating the push rod; 23. a direction-changing cavity; 24. a bevel gear; 25. a clamping block; 26. detecting the outer slide bar; 27. detecting the push rod; 28. a transmission cavity block; 29. a follower wheel; 30. a turning transmission rod; 31. a rope; 32. a reel; 33. an open slide plate; 34. a bearing; 35. a connecting plate; 36. a collection chamber; 37. a motor frame; 38. a motor; 39. a differential mechanism; 40. a shaft sleeve; 41. an output shaft; 42. a water permeable membrane; 43. a tilting frame; 44. a water collector; 45. stirring the leaves; 46. a gear transmission case.
Detailed Description
The following are specific embodiments of the present invention and are further described with reference to the drawings, but the present invention is not limited to these embodiments.
As shown in fig. 1, a probiotic culture equipment, including casing 10, a serial communication port, be equipped with closing plate 18 on the casing 10, be equipped with sealing device on the closing plate 18, sealing device is used for guaranteeing the inside seal of cavity with outside isolated opening with the cavity, fixed being equipped with on the lateral wall under the casing 10 collects chamber 36, be equipped with tilting device on collecting chamber 36, tilting device is used for rocking the inside cavity of cavity, tilting device is including rotating motor frame 37 on collecting chamber 36, motor frame 37 internal fixation is equipped with motor 38, the output of motor 38 is equipped with output shaft 41, be equipped with on the output shaft 41 and turn over the device of stirring, it is used for stirring the turning over of the nutrition base of bacterial culture to turn over the device of stirring, be equipped with detection device on the lateral wall about the casing 10, detection device is used for carrying out the detection to the inside bacterial colony of cavity.
As shown in fig. 2 and 3, the sealing device includes a thick sealing pad 20 fixed on the lower sidewall of the sealing plate 18, a balancing device is arranged on the thick sealing pad 20, the balancing device is used for adjusting dynamic balance of various parameters inside the cavity, a locking nail 19 is arranged on the sealing plate 18, a clamping device is arranged outside the locking nail 19, and the clamping device is used for ensuring stability of the sealing device to clamp the stabilizing device.
As shown in fig. 2, 3 and 5, the balancing device includes a gas controller 12 fixed on a sealing plate 18, a humidification cooler 13 is fixedly arranged on the sealing plate 18, a pipeline 14 is arranged at the lower ends of the gas controller 12 and the humidification cooler 13, the pipeline 14 extends downwards and extends into a thick sealing gasket 20, a lead screw 15 is fixedly arranged in the thick sealing gasket 20, an electric slider 16 is slidably arranged on the lead screw 15, a detector 17 is fixedly arranged in the lower side wall of the thick sealing gasket 20, the detector 17 is used for detecting the air pressure, the temperature and the humidity in the cavity and transmitting an electric signal to the electric slider 16, the electric slider 16 is operated to slide on the lead screw 15, an opening is arranged on the electric slider 16, and when the opening coincides with the pipeline 14, the opening together with the cavity, the gas controller 12 and the humidification cooler 13.
As shown in fig. 2, 3 and 5, the clamping device includes a clamping slider 21 sliding on the lower end of the sealing plate 18, the clamping slider 21 can slide into the side wall of the housing 10, a rotating push rod 22 is rotatably disposed in the clamping slider 21, a bevel gear is disposed near the axial center end of the rotating push rod 22, a turning cavity 23 is disposed near the axial center end of the clamping slider 21, a bevel gear 24 is rotatably disposed in the turning cavity 23, a clamping block 25 is rotatably disposed on the bevel gear 24, the bevel gear 24 can be engaged with the rotating push rod 22 near the axial center end for transmission, when the bevel gear 24 rotates, the clamping block 25 is driven to rotate, and the clamping block 25 is ejected or retracted outwards.
As shown in fig. 2 and 3, the tilting device comprises a collecting chamber 36, a tilting frame 43 is fixedly arranged on the collecting chamber 36, a sliding groove is arranged on the tilting frame 43, an incubator 11 is arranged on the tilting frame 43 in a sliding manner, the incubator 11 can swing back and forth on the tilting frame 43, and a recovery device is arranged on the collecting chamber 36 and used for excessive moisture generated in the bacteria culture process.
As shown in FIG. 3, the recovering device comprises a water collector 44 fixed on the collecting chamber 36, a water permeable membrane 42 is fixed on the lower side wall of the incubator 11, and the excessive water can flow into the collecting chamber 36 through the water collector 44 through the water permeable membrane 42.
As shown in fig. 3, the stirring device includes a differential 39 fixed on an output shaft 41, a shaft sleeve 40 is fixedly arranged outside the differential 39, the rotation frequency of the shaft sleeve 40 and the rotation frequency of the output shaft 41 can be different through the differential 39, a gear transmission case 46 is fixedly arranged on the shaft sleeve 40, stirring blades 45 are rotatably arranged outside the gear transmission case 46, and the rotation force on the output shaft 41 is transmitted to the stirring blades 45 through the gear transmission case 46.
As shown in fig. 2, 3 and 4, the detecting device includes an outer detecting slide bar 26 sliding on the left and right side walls of the casing 10, a detecting push rod 27 is slidably disposed in the outer detecting slide bar 26, a connecting plate 35 is fixedly disposed on the left and right inner side walls of the casing 10, a transmission cavity block 28 is disposed in the connecting plate 35, a follower wheel 29 is rotatably disposed in the transmission cavity block 28, a reel 32 is rotatably disposed in the incubator 11, an opening slide plate 33 is slidably disposed in the incubator 11, a bearing 34 is fixedly disposed in the incubator 11, a direction-changing transmission rod 30 is rotatably disposed on the bearing 34, a rope 31 is connected between the opening slide plate 33 and the reel 32 and between the direction-changing transmission rod 30, and the outer detecting slide bar 26 can drive the follower wheel 29 to rotate.
When the device is used for culturing probiotics, a culture medium is placed in the incubator 11, the interior of the shell 10 is sealed through the sealing plate 18, the interior air is adjusted through the humidifying cooler 13, oxygen is extracted and nitrogen is filled, parameters such as the interior air pressure, the temperature and the humidity are detected constantly, parameters which are useful for culturing the probiotics in the shell 10 are automatically adjusted, the parameters can slide inwards through the detection outer sliding rod 26, the detection push rod 27 extends into the incubator 11, the probiotics in the incubator 11 is detected, the cultured probiotics are guaranteed to reach the standard, the cultured probiotics are tilted and stirred in the incubator 11 in the process of culturing the probiotics, the problems that the temperature of the center of the interior culture medium is too high and the like are solved, and meanwhile, excessive moisture is filtered in the stirring process, so that the propagation of the probiotics is not influenced by excessive moisture.
When the sealing device is used, the rotating push rod 22 is pushed, the clamping slide block 21 is integrally pushed into the side wall of the shell 10, the rotating push rod 22 rotates, the rotating push rod 22 drives the bevel gear 24 to rotate, the bevel gear 24 rotates to eject the clamping block 25, the clamping block 25 clamps the clamping slide block 21 in the shell 10, the clamping slide block 21 also clamps the locking nail 19 to prevent the locking nail 19 from sliding up and down in the vertical direction, the effect that the sealing plate 18 covers and seals the shell 10 is achieved, after the sealing, the detector 17 is started to dynamically monitor the inside of the cavity, when the temperature inside of the shell 10 is overhigh, the electric slide block 16 slides to the right side, the opening on the electric slide block 16 is connected with the pipeline 14 below the humidifying and cooling device 13 to spray atomized water vapor for cooling, when the air pressure inside the shell 10 is unstable, the electric slide block 16 slides to the left side, the opening on the electric slide block 16 is connected with the pipeline 14 below the air controller 12, control the inside atmospheric pressure dynamic balance of casing 10 through gas controller 12, prevent when detecting that nitrogen gas is outwards revealed, lead to outside oxygen to get into, can guarantee the inside sealed effect of device to the effect of the inside dynamic balance of maintenance device can provide better living reproduction environment for the probiotic.
The incubator 11 is tilted at regular time intervals, and the incubator 11 is tilted back and forth on the tilting frame 43 to facilitate the downward flow of the excess water, into the collection chamber 36 through the water collector 44, anaerobic respiration and moisture generation are generated in the process of probiotic reproduction, and the collection of the anaerobic respiration and moisture generation is required, and the motor 38 is started, the motor 38 drives the output shaft 41 to rotate, and the effect of controlling the rotating speed of the shaft sleeve 40 is achieved through the differential effect of the differential 39, so that the rotating speed of the shaft sleeve 40 and the rotating speed of the output shaft 41 are different, and output shaft 41 rotation efficiency is faster than axle sleeve 40, when output shaft 41 rotated, again through gear transmission case 46 transmission, will drive stirring leaf 45 and rotate, stirring leaf 45 rotates and can carry out the stirring of the inside culture medium of incubator 11, avoids incubator 11 inside culture medium central temperature too high, leads to the probiotic to breed not good.
In the in-process of living fungus reproduction, carry out the detection to the bacterial colony, detect outer slide bar 26 and wholly slide to casing 10 inside, the in-process that detects outer slide bar 26 and slide in can drive follow-up wheel 29 and rotate, follow-up wheel 29 rotates and can drive diversion transfer line 30 and rotate, diversion transfer line 30 can carry out tightening up of rope 31, open slide 33 can slide to keeping away from axle center end department after rope 31 tightens up, it can enter into inside culture apparatus 11 to detect outer slide bar 26, stretch into inside culture apparatus 11 with detection push rod 27 afterwards, carry out the bacterial colony sampling inside culture apparatus 11, can guarantee at the in-process of cultivateing living fungus, the fixed point is regularly surveyed the appearance and is detected, guarantee the quality and the quantity that living fungus was cultivateed, avoid cultivateing the too much or the too few condition of living fungus.
Compared with the prior art, the probiotic culture equipment has the following advantages:
1. prevent when detecting, nitrogen gas is outwards revealed, leads to outside oxygen to get into, can guarantee the inside sealed effect of device to the effect of the inside dynamic balance of maintenance device can provide better living reproduction environment for the probiotic.
2. The stirring blades rotate to stir the culture medium in the incubator, so that the problem that the center temperature of the culture medium in the incubator is too high, and the probiotics is not well bred is avoided.
3. Carry out the bacterial colony sampling to the inside of incubator, can guarantee to survey the appearance and detect regularly fixed point at the in-process of cultivateing the probiotic, guarantee the quality and the quantity that the probiotic was cultivateed, avoid cultivateing the too much or too little condition of probiotic.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (8)
1. The probiotic culture equipment comprises a shell (10) and is characterized in that a sealing plate (18) is arranged on the shell (10), a sealing device is arranged on the sealing plate (18) and used for isolating the inside and the outside of a cavity to ensure the airtightness of the inside of the cavity, a collecting cavity (36) is fixedly arranged on the lower side wall of the shell (10), a tilting device is arranged on the collecting cavity (36) and used for shaking the cavity inside the cavity, the tilting device comprises a motor frame (37) rotating on the collecting cavity (36), a motor (38) is fixedly arranged in the motor frame (37), an output shaft (41) is arranged at the output end of the motor (38), a stirring device is arranged on the output shaft (41) and used for stirring a nutrient medium cultured by bacteria, and detection devices are arranged on the left side wall and the right side wall of the shell (10), the detection device is used for detecting bacterial colonies in the cavity.
2. A probiotic culture apparatus according to claim 1, characterised in that: sealing device is including fixing thick sealed pad (20) on closing plate (18) lower lateral wall, be equipped with balancing unit on thick sealed pad (20), balancing unit is used for adjusting the inside various parameter dynamic balance of cavity, be equipped with lock nail (19) on closing plate (18), the lock nail (19) outside is equipped with chucking device, and chucking device is used for guaranteeing sealing device stability with the stabilising arrangement chucking.
3. A probiotic culture apparatus according to claim 2, characterised in that: the balancing device comprises a gas controller (12) fixed on a sealing plate (18), a humidification cooler (13) is fixedly arranged on the sealing plate (18), the gas controller (12) and the lower end of the humidification cooler (13) are provided with pipelines (14), the pipelines (14) extend downwards and extend into the thick sealing gasket (20), a lead screw (15) is fixedly arranged in the thick sealing gasket (20), an electric slider (16) is arranged on the lead screw (15) in a sliding manner, a detector (17) is fixedly arranged in the lower side wall of the thick sealing gasket (20), the detector (17) is used for detecting the air pressure, the temperature and the humidity in the cavity and transmitting an electric signal to the electric slider (16) to operate the electric slider (16) to slide on the lead screw (15), an opening is arranged on the electric slider (16), and when the opening is coincided with the pipelines (14), will be connected with the chamber and the gas controller (12) and the humidification and cooling device (13).
4. A probiotic culture apparatus according to claim 3, characterised in that: the clamping device comprises a clamping slide block (21) which slides at the lower end of the sealing plate (18), the clamping slide block (21) can slide into the side wall of the shell (10), a rotating push rod (22) is arranged in the clamping slide block (21) in a rotating mode, bevel teeth are arranged at the end, close to the axial center, of the rotating push rod (22), a turning cavity (23) is arranged at the end, close to the axial center, of the clamping slide block (21), a bevel gear (24) is arranged in the turning cavity (23) in a rotating mode, a clamping block (25) is arranged on the bevel gear (24) in a rotating mode, the bevel gear (24) can be in meshing transmission with the end, close to the axial center, of the rotating push rod (22), and when the bevel gear (24) rotates, the clamping block (25) can be driven to rotate, and the clamping block (25) can be outwards ejected or withdrawn.
5. A probiotic culture apparatus according to claim 4, characterised in that: the tilting device comprises a tilting frame (43) fixedly arranged on the collection cavity (36), a sliding groove is formed in the tilting frame (43), an incubator (11) is arranged on the tilting frame (43) in a sliding mode, the incubator (11) can swing back and forth on the tilting frame (43), a recovery device is arranged on the collection cavity (36), and the recovery device is used for carrying out excessive moisture generated in the bacteria culture process.
6. A probiotic culture apparatus according to claim 5, characterised in that: the recovery device comprises a water collector (44) fixed on the collection cavity (36), a water permeable membrane (42) is fixedly arranged on the lower side wall of the incubator (11), and redundant water can flow into the collection cavity (36) through the water collector (44) through the water permeable membrane (42).
7. A probiotic culture apparatus according to claim 6, characterised in that: the stirring device comprises a differential (39) fixed on an output shaft (41), a shaft sleeve (40) is fixedly arranged on the outer side of the differential (39), the differential (39) can reach that the shaft sleeve (40) and the output shaft (41) have different rotating frequencies, a gear transmission box (46) is fixedly arranged on the shaft sleeve (40), a stirring blade (45) is arranged on the outer side of the gear transmission box (46) in a rotating mode, and rotating force on the output shaft (41) is transmitted to the stirring blade (45) through the gear transmission box (46).
8. The probiotic culture apparatus of claim 7, wherein: the detection device comprises a detection outer sliding rod (26) sliding on the left side wall and the right side wall of the shell (10), a detection push rod (27) is arranged in the detection outer slide bar (26) in a sliding way, connecting plates (35) are fixedly arranged on the left inner side wall and the right inner side wall of the shell (10), a transmission cavity block (28) is arranged in the connecting plate (35), a follower wheel (29) is rotationally arranged in the transmission cavity block (28), a reel (32) is rotationally arranged in the incubator (11), an opening sliding plate (33) is arranged in the incubator (11) in a sliding way, a bearing (34) is fixedly arranged in the incubator (11), a turning transmission rod (30) is rotatably arranged on the bearing (34), a rope (31) is connected between the opening slide plate (33) and the reel (32) as well as the direction-changing transmission rod (30), the detection outer slide bar (26) can drive the follower wheel (29) to rotate.
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