CN206916156U - The cell culture apparatus of temperature-controllable - Google Patents

Abstract

It the utility model is related to field of cell culture, a kind of more particularly to cell culture apparatus of temperature-controllable, cell observation cover including cell culture chamber, on the cell culture chamber, the cell culture assembly in the cell culture room, and a cell culture temperature control assembly；Wherein cell culture chamber, including a base and the supporting frame that is connected with the base；Cell observation cover is fixedly connected with the upper end of support frame as described above body, and one is provided with the top of the cell observation cover and is suitable to the observation port that microscope probes；Cell culture assembly, on the base, and including containing liquid vessel, and it is placed in the Sheng liquid vessel inner cell culturing rack；The Cell culture rack includes carrier and the carrier film being fixed on carrier；And cell culture temperature control assembly, on supporting frame inwall, including thermoregulator and temperature inductor.The utility model is easy to control cell culture temperature, reduces cell culture difficulty.

Description

The cell culture apparatus of temperature-controllable

Technical field

It the utility model is related to field of cell culture, more particularly to a kind of cell culture apparatus of temperature-controllable.

Background technology

In biotechnology, cell culture is an essential process.Cell culture is usually to put cell Enter blake bottle, then be put into incubator and carry out.During cell culture blake bottle can be taken out from incubator and be put into microscope Under to observe the growing state of cell.Under normal circumstances because the temperature of incubator is higher than room temperature, the vapor in blake bottle Water smoke can be condensed into after quenching on bottle top.Water smoke can cause light diffusing reflection so that microscope imaging is unclear.

And because this carrier film is relatively thin, lighter weight, therefore added in culture vessel during cell solution, carrying Film often produces suspension and fold, increases follow-up cell culture and detection difficulty.

Utility model content

The purpose of this utility model is to provide a kind of cell culture apparatus of temperature-controllable, solves in cell culture and is easy to Cell culture temperature is controlled, and reduces the technical problem of cell culture difficulty.

In order to solve the above-mentioned technical problem, the utility model provides technical scheme and is：A kind of cell training of temperature-controllable Support device, including cell culture chamber, the cell observation cover on the cell culture chamber, in the cell culture room Cell culture assembly, and a cell culture temperature control assembly；Wherein cell culture chamber, including a base and with the base connect The supporting frame connect；Cell observation cover is fixedly connected with the upper end of support frame as described above body, and is provided with the top of the cell observation cover One is suitable to the observation port that microscope probes；Cell culture assembly, on the base, and including containing liquid vessel, and it is placed in The Sheng liquid vessel inner cell culturing rack；The Cell culture rack includes carrier and the carrier film being fixed on carrier；And Cell culture temperature control assembly, on supporting frame inwall, including thermoregulator and temperature inductor.

Further, the top of the cell observation cover is by the right baffle-plate that can be overturn around center line located at arranged on left and right sides Formed with right shell body, and the relative one side of left and right baffle is provided with symmetrical semicircle be open；I.e. left and right baffle closes Just to form described observation port.

Further, described supporting frame is hollow column structure, wherein being offered respectively on relative two sides One circular hole, the circular hole are respectively connected with the operating gloves inside support frame as described above body.

Further, for the Sheng liquid vessel to contain liquid plate, the Sheng liquid plate is provided with some Sheng fluid apertures, and each contains fluid apertures Inside it is equipped with described Cell culture rack.

Further, the carrier is cyclic structure, and the carrier lower ending opening face is to contain liquid vessel for stretching into Interior carrying mouth, what it is located at the carrier upper end open edge is for being suspended on the suspension part contained on fluid apertures wall；Wherein The suspension part outward flange is provided with a pair of mutually symmetrical with handles.

Further, the Cell culture rack is also sheathed on the dead ring at carrying mouth for fixing carrier film including one； I.e. described dead ring engagement sleeves are pressed in institute in one week at carrying mouth, by the edge part of the carrier film along the carrying mouth In the outer side wall surface for stating carrying mouth.

Further, the Sheng liquid vessel use IT0 electro-conductive glass, and its relative both ends connects a bronze medal guide card respectively.

Further, one end is additionally provided with support frame as described above body on wall to be electrically connected with the copper guide card, the other end and institute State the D.C. regulated power supply of thermoregulator electric connection.

Further, described temperature inductor includes the temperature sense pin being electrically connected with the thermoregulator；Institute State temperature sense pin and stretch into the Sheng liquid vessel temperature transmitted in Sheng liquid vessel.

Further alternative, the thermoregulator uses REX-C900 relay electronic temperature control instrument.The utility model Have the beneficial effect that：Use cell observation cover to provide the environment of observation of constant temperature for cell culture, avoid fog from producing, and Convenient operation and observation.And operating gloves are set on supporting frame, and required angle can be adjusted by facilitating during micro- sem observation.

Further, coordinated in the cell culture assembly using carrying mouth and carrier film, and carrier upper end is hung On liquid vessel are contained, carrying mouth, which stretches into, to be contained in liquid vessel, and the cell for alloing uniformly to be attached on carrier film fully absorbs Sheng liquid The nutrient of solution in vessel.

Further, the Sheng liquid vessel made using electro-conductive glass can make its heating, change cultivation temperature, and lead to Temperature needed for the regulation of excess temperature adjuster, it is simple in construction, it is easy for installation.

Brief description of the drawings

The utility model is further illustrated with reference to the accompanying drawings and examples.

Fig. 1 is the overall structure diagram of the cell culture apparatus of temperature-controllable of the present utility model；

Fig. 2 is the structural representation of cell observation cover of the present utility model；

Fig. 3 is the structural representation of Cell culture rack of the present utility model；

Fig. 4 is the structural representation of dead ring of the present utility model.

In figure：Cell culture chamber 1, cell observation cover 2, cell culture assembly 3, cell culture temperature control assembly 4, base 5th, supporting frame 6, observation port 7, Sheng liquid vessel 8, Cell culture rack 9, carrier 10, carrier film 11, right baffle-plate 12, right shell body 13rd, semicircle opening 14, circular hole 15, operating gloves 16, contain fluid apertures 17, carrying mouth 18, suspension part 19, handle 20, dead ring 21, Projection 22, copper guide card 23, D.C. regulated power supply 24, temperature sense pin 25, lead 26, temperature sensor 27.

Embodiment

Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made The every other embodiment obtained, belong to the scope of the utility model protection.

In description of the present utility model, it is to be understood that term " upper end ", " lower end ", " relative ", " left side ", " right side ", " top ", " bottom ", " interior ", the orientation of the instruction such as " outer " or position relationship be to be closed based on orientation shown in the drawings or position System, it is for only for ease of and describes the utility model and simplified description, rather than the device or element of instruction or hint meaning is necessary With specific orientation, with specific azimuth configuration and operation, therefore it is not intended that to limitation of the present utility model.In this reality With in new description, unless otherwise indicated, " some " are meant that two or more.

Embodiment 1：

As shown in Figures 1 to 4, the utility model provides a kind of cell culture apparatus of temperature-controllable, including cell training Support room 1, the cell observation cover 2 on the cell culture chamber 1, the cell culture assembly in the cell culture chamber 1 3, and a cell culture temperature control assembly 4；Wherein cell culture chamber 1 includes a base 5 and the support being connected with the base 5 Support body 6；Cell observation cover 2 is fixedly connected with the upper end of support frame as described above body 6, and the top of the cell observation cover 2 is provided with one and fitted In the observation port 7 that microscope probes；Use cell observation cover 2 to provide the environment of observation of constant temperature for cell culture, avoid mist Gas produces, and convenient operation and observation.

Optionally, the base 5 can use such as, but not limited to cup dolly 5 or direction base 5 etc..

Specifically, cell culture assembly 3 is located on the base 5, and including containing liquid vessel 8, and it is placed in the Sheng liquid The inner cell culturing rack 9 of vessel 8；The Cell culture rack 9 includes carrier 10 and the carrier film 11 being fixed on carrier 10；And Cell culture temperature control assembly 4 on the inwall of supporting frame 6 includes thermoregulator and temperature inductor.

Carrier 10 is used to fix carrier film 11, therefore in order to prevent the competent cell on carrier film 11 from migrating to carrier The accuracy of the subsequently cell detection on carrier film 11 is have impact on 10, carrier 10 can be made as being unfavorable for cell life The structure of long breeding.In order to which carrier 10 to be made as being unfavorable for the structure of cell growth breeding, it is preferable that carrier 10 is by gathering Lactic acid fused glass pellet, because PLA has hydrophobicity, without hydrophily, therefore cell is difficult to be attached on this material Growth, therefore cannot be used for supporting cell, competent cell is thus prevented to the transfer on carrier 10.Moreover, fusion sediment One kind that forming technique belongs in 3D printing technique, have the advantages that shaped article precision is high, surface quality is good, simultaneously because poly- Lactate material has the advantages that nonhazardous, makes have precision height, surface matter by the carrier 10 after PLA fused glass pellet Measure, the characteristic such as non-environmental-pollution.

Optionally, carrier film 11 is molded from electrostatic spinning process, and the received vector of electrostatic spinning process is buffer solution, quiet Carrier film 11 after Electrospun shaping may float on this buffer solution due to lighter weight, thinner thickness, only need to use tweezer Son is by carrier film 11 by i.e. achievable carrier film 11 and the separation of buffer solution, the hole of carrier film 11 are taken out and cleaned up in solution Gap size does not change.It is additionally, since conventional buffer solution to act on cytotoxic evil, therefore is taken out in buffer solution Fail to wash this solution completely after carrier film 11, also will not cell growth production when using this carrier film 11 carrying cell Raw large effect.

Specifically, as shown in Fig. 2 the top of cell observation cover 2 by located at arranged on left and right sides can around center line overturn a left side Baffle plate 12 and right shell body 13 form, and the relative one side of left and right baffle is provided with and symmetrical semicircle is open 14；I.e. left, Right shell body just forms described observation port 7 altogether.Observation port 7 passes through suitable for microscopical eyepiece.And on the observation port 7 Provided with a door, observation port 7 is blocked during the cell culture do not observed, forms the culture space of cell culture.

Described supporting frame 6 is hollow column structure, wherein a circular hole 15 is offered on relative two sides respectively, The circular hole 15 is respectively connected with the operating gloves 16 inside support frame as described above body 6.

Wherein optional, such as, but not limited to cylinder, the four prisms cylinder etc. coordinated with base 5 can be selected in supporting frame 6.

When being observed, the door of observation port 7 is taken away, eyepiece is observed through observation port 7, passes through operating gloves 16 contain the angular adjustment of liquid vessel 8 and microscope ocular.

The Sheng liquid vessel 8 are Sheng liquid plate, and the Sheng liquid plate is provided with some Sheng fluid apertures 17, and in each Sheng fluid apertures 17 Provided with described Cell culture rack 9.

As shown in figure 3, carrier 10 can use cyclic structure, the structure of inner hollow is formed, wherein under carrier 10 End opening face is for stretching into the carrying mouth 18 contained in liquid vessel 8, carries the shape of mouth 18 and can use and such as, but not limited to justifies Shape, square, polygon etc..What it is located at the upper end open edge of carrier 10 is for being suspended on the suspension contained on the wall of fluid apertures 17 Portion 19, the suspension part 19 formed, which can facilitate, takes out Cell culture rack 9, and tweezer in liquid vessel 8 is contained to avoid being stretched into tweezers Take, reduce operation difficulty.

Preferably, the outward flange of suspension part 19 is provided with mutually symmetrical with a pair of handles 20.Handle can be lived by tweezers tweezer Cell culture rack 9 is taken out or is put into and contained in liquid vessel 8 by hand 20, because handle 20 is arranged on the outward flange of suspension part 19, to Overhang, if therefore in order to prevent that the dust water in air from entering in vessel and need to be set in lid at the upper end open of tubular structure Gai Shi, handle 20 will not produce interference with lid on this.

Specific optional, carrier 10 is such as, but not limited to frustum type tubular structure, cylindrical barrel knot using structure Structure or prismatic tubular structure.But in order that the carrying mouth 18 on carrier 10 can smoothly enter the Sheng fluid apertures for containing liquid vessel 8 In 17, avoid colliding with each other and cause carrier film 11 to damage, therefore preferably carrier 10 is frustum type tubular structure, and frustum type tube Less opening down, the formation carrying mouth 18 of shape structure, because the small top and big bottom structure of frustum type tubular structure is special Property, one end area of the carrying mouth 18 of setting is small, can be stretched into easier in liquid container, protect carrier film 11 not to be damaged Wound.

Preferably, as shown in figure 4, the Cell culture rack 9 is also sheathed at carrying mouth 18 including one is used for fixed carrying The dead ring 21 of film 11；The i.e. described engagement sleeves of dead ring 21 are at carrying mouth 18, by the edge part of the carrier film 11 along institute State being pressed in for one week in the outer side wall surface of the carrying mouth 18 for carrying mouth 18.

Specifically, the inwall of dead ring 21 is provided with each and every one some projections 22 in one week.When the engagement sleeves of dead ring 21 are located at carrying When on the wall outer surface of mouth 18, this projection 22 is in impaction state, thus can apply one to the wall outer surface for carrying mouth 18 Fixed extruding force is with the edge one week of packed cell culture tunica fibrosa.This is simple in construction, easily realizes.

Carrying mouth 18 is used for the outer surface that the edge of carrier film 11 is fixed on to the carrying side wall of mouth 18, by with carrying mouth The dead ring 21 that 18 lateral walls coordinate compresses the edge of carrier film 11, ensures that carrier film 11 will not produce wrinkle when cultivating cell Contracting, and cell can be uniformly attached on carrier film 11, be absorbed to each cell and contained supporting for nutrient solution in liquid vessel 8 Point.Reduce the difficulty of cell culture.

Wherein, mode, the side of carrier film 11 specifically are fixedly connected with the one kind at the edge of carrier film 11 as carrying mouth 18 It was adhered to along one week by medical adhesive at carrying mouth 18.This is simple in construction, and medical adhesive has no toxic side effect, to cell Growth will not have an impact.

The Sheng liquid vessel 8 use IT0 electro-conductive glass, and its relative both ends connects a bronze medal guide card 23 respectively.In order to avoid Other make contains liquid vessel 8, saving cost, and the area of carrier film 11 should often use culture vessel with laboratory in Cell culture rack 9 Floor space it is equal, the Sheng liquid vessel 8 that what thus contrast experiment used contain liquid vessel 8 and laboratory can be selected to commonly use, can avoid Make in addition, so as to save cost.

One end is additionally provided with the inwall of support frame as described above body 6 and is electrically connected with the other end and temperature tune with the copper guide card 23 Save the D.C. regulated power supply 24 that device is electrically connected with.D.C. regulated power supply 24 transmits electric current to liquid vessel 8 are contained, and produces Sheng liquid vessel 8 Heat amount, the nutrient solution in heating vessel.D.C. regulated power supply 24 stretches out two leads 26, and two leads 26 and copper guide card 23 Connection end is equipped with the clip for accommodating copper guide card 23.

Described temperature inductor includes the temperature sense pin 25 being electrically connected with the thermoregulator；The temperature sense Answer pin 25 to stretch into the Sheng liquid vessel 8 and transmit the temperature contained in liquid vessel 8.

Wherein, temperature sense pin 25 uses Heat Conduction Material, and the temperature sense pin 25 is another in liquid with respect to stretching into One end is also associated with the temperature sensor 27 being electrically connected with thermoregulator.Temperature sense pin 25 rotates with temperature sensor 27 Connection.To avoid temperature sense from being impacted for nutrient solution, used temperature sense pin is nontoxic heat-conducting plastic.

The principle of regulation temperature is that the temperature sensed by temperature sense pin 25 passes to temperature sensor, TEMP Device feeds back to thermoregulator, and temperature exceedes setting range, just controls D.C. regulated power supply 24 to stop output, ensures temperature control In optimum range.

Wherein optional, temperature sensor 27 uses K-type armoured thermocouple WRNK-191 probe thermocouple temperature sensors 27。

Optionally, thermoregulator is using such as, but not limited to REX-C900 relays electronic temperature control instrument.

It is described above, only specific embodiment of the present utility model, but the scope of protection of the utility model is not limited to In this, any one skilled in the art can readily occur in change in the technical scope that the utility model discloses Or replace, it should all cover within the scope of protection of the utility model.Therefore, the scope of protection of the utility model should be with the power The protection domain that profit requires is defined.

Claims (10)

1. a kind of cell culture apparatus of temperature-controllable, it is characterised in that including cell culture chamber, located at the cell culture chamber On cell observation cover, the cell culture assembly in the cell culture room, an and cell culture temperature control assembly； Wherein cell culture chamber, including a base and the supporting frame that is connected with the base；Cell observation cover and support frame as described above body Upper end is fixedly connected, and one is provided with the top of the cell observation cover and is suitable to the observation port that microscope probes；Cell culture assembly, if In on the base, and including containing liquid vessel, and it is placed in the Sheng liquid vessel inner cell culturing rack；The Cell culture rack includes Carrier and the carrier film being fixed on carrier；And cell culture temperature control assembly, on supporting frame inwall, bag Include thermoregulator and temperature inductor.

2. the cell culture apparatus of temperature-controllable according to claim 1, it is characterised in that the top of the cell observation cover Portion is made up of the right baffle-plate and right shell body that can be overturn around center line located at arranged on left and right sides, and the side that left and right baffle is relative Face is provided with symmetrical semicircle opening；I.e. left and right baffle just forms described observation port altogether.

3. the cell culture apparatus of temperature-controllable according to claim 1, it is characterised in that during described supporting frame is Empty column structure, wherein offering a circular hole on relative two sides respectively, the circular hole is respectively connected with one and is located at the branch Operating gloves inside support body.

4. the cell culture apparatus of temperature-controllable according to claim 1, it is characterised in that the Sheng liquid vessel are Sheng liquid Plate, the Sheng liquid plate is provided with some Sheng fluid apertures, and each contain is equipped with described Cell culture rack in fluid apertures.

5. the cell culture apparatus of temperature-controllable according to claim 4, it is characterised in that the carrier is ring-type knot Structure, the carrier lower ending opening face are for stretching into the carrying mouth contained in liquid vessel, located at the carrier upper end open side At edge is for being suspended on the suspension part contained on fluid apertures wall；Wherein described suspension part outward flange is provided with mutually symmetrical with a pair Hand.

6. the cell culture apparatus of temperature-controllable according to claim 5, it is characterised in that the Cell culture rack is also wrapped Include one and be sheathed on the dead ring carried at mouth for fixing carrier film；I.e. described dead ring engagement sleeves are at carrying mouth, by institute State edge part being pressed in for one week in the outer side wall surface of the carrying mouth along the carrying mouth of carrier film.

7. the cell culture apparatus of the temperature-controllable according to any one of claim 1 to 6, it is characterised in that the Sheng liquid Vessel use IT0 electro-conductive glass, and its relative both ends connects a bronze medal guide card respectively.

8. the cell culture apparatus of temperature-controllable according to claim 7, it is characterised in that in support frame as described above body on wall It is additionally provided with one end to be electrically connected with the copper guide card, the D.C. regulated power supply that the other end is electrically connected with the thermoregulator.

9. the cell culture apparatus of temperature-controllable according to claim 8, it is characterised in that described temperature inductor bag Include the temperature sense pin being electrically connected with the thermoregulator；The temperature sense pin is stretched into the Sheng liquid vessel to transmit and contained Temperature in liquid vessel.

10. the cell culture apparatus of temperature-controllable according to claim 9, it is characterised in that the thermoregulator is adopted With REX-C900 relay electronic temperature control instrument.