CN111103284A - Constant temperature cell imaging system - Google Patents
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- CN111103284A CN111103284A CN201911113196.4A CN201911113196A CN111103284A CN 111103284 A CN111103284 A CN 111103284A CN 201911113196 A CN201911113196 A CN 201911113196A CN 111103284 A CN111103284 A CN 111103284A
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- 238000003384 imaging method Methods 0.000 title claims abstract description 49
- 238000004113 cell culture Methods 0.000 claims abstract description 65
- 210000004027 cell Anatomy 0.000 claims description 79
- 239000011521 glass Substances 0.000 claims description 33
- 238000010438 heat treatment Methods 0.000 claims description 23
- 238000012545 processing Methods 0.000 claims description 21
- 238000004891 communication Methods 0.000 claims description 17
- 230000007246 mechanism Effects 0.000 claims description 14
- 210000005056 cell body Anatomy 0.000 claims description 12
- 238000005192 partition Methods 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 7
- 238000005485 electric heating Methods 0.000 claims description 6
- 230000002070 germicidal effect Effects 0.000 claims description 5
- 230000033001 locomotion Effects 0.000 claims description 5
- 238000010295 mobile communication Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000006073 displacement reaction Methods 0.000 claims 1
- 230000007306 turnover Effects 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 17
- 230000008569 process Effects 0.000 abstract description 14
- 230000001766 physiological effect Effects 0.000 abstract description 4
- 230000024245 cell differentiation Effects 0.000 description 7
- 230000000694 effects Effects 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 230000001464 adherent effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 230000006855 networking Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000003760 hair shine Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultraviolet radiation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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Abstract
The invention discloses a constant-temperature cell imaging system, which comprises a tank body, a constant-temperature inner cavity, a constant-temperature loop and a trinocular biological image microscope, wherein: the invention can observe the whole process of cell culture by a trinocular biological image microscope while keeping the physiological activity of the cells, can store the characteristic information of the cells, can watch the state of the individual cells at any time by a mobile phone, can observe the whole process of cell culture by the mobile phone, and can be displayed on a display screen communicated with mobile terminals such as the mobile phone and the like when needed.
Description
Technical Field
The invention belongs to the technical field of detection equipment, and particularly relates to a constant-temperature cell imaging system.
Background
In the field of biological medicine, the application of a microscopic scanning trinocular biological image microscope is very wide, in the application process, a sample to be microscopically scanned is often required to be placed on an objective table, the objective table drives the sample to move, so that all parts of the sample can sequentially pass through a focusing plane of an objective lens, then all points on the sample can be respectively imaged through the objective lens, and finally the imaging of all points is spliced to obtain the image of the whole sample, thereby completing the microscopic scanning of the sample.
At present, the microscopic imaging of cells usually needs to place the cells on a slide holder first, then place the slide holder on an objective table, and correspondingly find the sample on the slide holder and clearly show the image of the cells through the movement of the objective table along an x axis and/or a y axis and the continuous focusing of a camera.
However, when microscopic imaging is performed in the process of cell differentiation and culture, most cells can be differentiated under a certain temperature condition, but at present, the microscopic imaging usually needs to be continuously focused and is performed by being directly exposed in the air, so that the risk of pollution is increased, the differentiation of the cells is greatly influenced by temperature change, the normal differentiation of the cells and the accuracy of the microscopic imaging are influenced, and the application limitation is large.
Disclosure of Invention
The present invention is directed to a constant temperature cell imaging system to solve the above-mentioned problems.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a constant temperature cell imaging system, includes cell body, constant temperature interior cavity, constant temperature return circuit, three mesh biological image microscopes, wherein: the cell body is internally provided with a constant-temperature inner cavity and a partition plate, an inner cavity is formed among the cell body, the constant-temperature inner cavity and the partition plate, a liquid medium is filled in the inner cavity, a constant-temperature loop is fixedly arranged at the bottom of the partition plate, a glass slide is arranged in the constant-temperature inner cavity, a light source is arranged below the glass slide, a cell culture tank and a shifting mechanism capable of horizontally shifting the cell culture tank are arranged above the glass slide, a trinocular biological image microscope is arranged above the cell body and is fixedly connected with a lifting device arranged behind the cell body through a connecting pipe, the trinocular biological image microscope is fixedly connected with a processing device capable of processing images and signals through a connecting cable, the processing device is fixedly connected with a display, a camera is arranged on the display, and a face recognition module and a communication module are arranged in the display, the communication module comprises a WiFi communication module and a mobile communication module, and the face recognition module can authenticate face information collected by the camera.
Preferably, the left side and the right side of the top of the tank body are correspondingly provided with tank doors, the tank doors are provided with tank door handles, one end of each tank door is movably connected with the tank body through a hinge column, the tank doors can rotate left and right relative to the tank body through the hinge columns, the tank doors on the left side and the right side are provided with connecting rings, the connecting rings correspondingly arranged on the left side and the right side can be combined into a hollow cylinder, the hollow cylinder can accommodate the three-eye biological image microscope to move up and down to enter and exit the tank body, and the tank body is provided with a sliding.
Preferably, the constant temperature loop includes the constant temperature pipeline, the constant temperature pipeline is intake and is held to run through the baffle and set up in the baffle left end just the constant temperature pipeline goes out the water outlet end and runs through the baffle and set up in the baffle right side distolaterally, last circulating pump, flow control valve and the heating pipe of being provided with of constant temperature pipeline, the inside electric heating wire who encircles the constant temperature pipeline outer wall that is provided with of heating pipe.
Preferably, the shifting mechanism comprises a motor, a telescopic column and a rubber push sheet, the motor is arranged on the inner wall of the constant-temperature inner cavity and is arranged at four ends of the constant-temperature inner cavity, the motor is arranged at the front end, the rear end, the left end and the right end of the constant-temperature inner cavity, the output end of the motor is correspondingly matched with the telescopic column, the other end of the telescopic column is fixedly connected with the rubber push sheet, and the motor can drive the telescopic column and the rubber push sheet to translate, so that the rubber push sheet can transversely and/or longitudinally move the cell culture tank, cells on the cell culture tank can be adjusted to corresponding positions, and microscopic imaging is.
Preferably, the slide glass is embedded in the constant-temperature inner cavity, the slide glass is transparent and can bear a transparent cell culture tank for placing cells to be cultured, a light source is arranged below the slide glass, light emitted by the light source can be focused by a condenser lens arranged above the light source, the focused light can be irradiated to the cells in the cell culture tank through the slide glass, and the light source is a light emitting diode corresponding to the slide glass array.
Preferably, the bottom of the trinocular biological image microscope is provided with an objective lens, the objective lens and the cell culture tank are arranged in parallel, the trinocular biological image microscope comprises a microscope tee joint, an eyepiece and a switching port are arranged on the microscope tee joint, the eyepiece is at a certain inclination angle and is arranged in front of the microscope tee joint, the switching port is arranged above the microscope tee joint, a CCD industrial camera is fixedly arranged above the switching port, and the CCD industrial camera can be electrically connected with a processing device through a connecting cable.
Preferably, the lifting device comprises a lifting motor, a lifting screw rod, a lifting bracket and a lifting slide block; the lifting screw rod is vertically arranged, the bottom of the lifting screw rod is correspondingly matched and connected with an output shaft of the lifting motor and can be driven by the output shaft of the lifting motor to rotate, the lifting slide block is matched with the lifting screw rod to form a threaded screw rod, a lifting support is arranged outside the lifting slide block in a covering mode, the lifting support and the lifting slide block are both of square cylinder structures, the lifting support is right opposite to the position of the connecting pipe, a lifting track is arranged at the position of the lifting screw rod, the rotation of the lifting screw rod can be correspondingly converted into the lifting slide block to move along the axial direction of the lifting screw rod, and therefore the three-mesh biological image microscope is driven to complete vertical lifting, and the three-mesh biological image microscope is enabled.
Preferably, the left side and the right side of the inner wall of the constant-temperature inner cavity are correspondingly provided with temperature sensors, the outer wall of the inner cavity is spirally and circularly provided with an electric heating wire, and the inner wall of the inner cavity is provided with an ultraviolet germicidal lamp which is arranged above the glass slide.
Preferably, the cell culture tank is of a hollow tank structure with an outer square and an inner circle, and a stirrer and a PH detector are arranged inside the cell culture tank.
Preferably, the inside PLC controlling means that is provided with of display just be provided with the display screen on the display, electric connection between display screen, PLC controlling means, light source, temperature sensor, heating pipe, elevator motor, PH detector, heating wire and the sterilamp.
The invention has the technical effects and advantages that: the constant-temperature cell imaging system comprises:
1. the cell in the cell culture tank can be adjusted to the corresponding position, the microscopic imaging is completed, and meanwhile, the focusing of the cell on the cell culture tank can be completed by the trinocular biological image microscope.
2. The inside of the constant-temperature inner cavity can be kept at a constant temperature through a constant-temperature circuit, so that the physiological activity of the cells can be maintained while the cell is microscopically imaged by the trinocular biological image microscope.
3. Can keep cell physiological activity through the whole process of the cultivation of three mesh biological image microscope observation cells simultaneously to can store the characteristic information of cell, and can retrieve when needs and show on the display screen, make things convenient for the later stage to look up.
4. Can effectively avoid and can effectively avoid the cell in the cell culture jar to be polluted by the bacterium through the setting of heating wire and sterilamp.
5. The characteristic information of the cells can be stored, the state of the individual cells can be watched at any time through the mobile phone, the whole process of the cell differentiation culture can be observed through the mobile phone, and the characteristic information can be displayed on a display screen communicated with a mobile terminal such as the mobile phone when needed.
6. The camera that sets up on the accessible display carries out face identification to operating personnel, prevents non-operating personnel maloperation to the camera that sets up on the accessible display supervises operating personnel in real time, prevents to take place the operation accident and can pass through the cell culture state in mobile terminal real time monitoring cell culture jar such as cell-phones.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic diagram of the present invention;
FIG. 3 is a cross-sectional view of the present invention;
FIG. 4 is a view showing the internal structure of the constant temperature internal chamber according to the present invention;
FIG. 5 is a block diagram of the present invention.
In the figure: 1-groove body, 2-constant temperature inner cavity, 3-constant temperature loop, 4-three-mesh biological image microscope, 5-lifting device, 6-connecting pipe, 7-connecting cable, 8-processing device, 9-display, 11-clapboard, 12-inner cavity, 13-groove door, 14-twisted column, 15-sliding groove, 121-temperature sensor, 122-electric heating wire, 123-ultraviolet sterilizing lamp, 131-groove door handle, 132-connecting ring, 133-hollow cylinder, 21-glass slide, 22-cell culture tank, 221-stirrer, 222-PH detector, 23-shifting mechanism, 24-light source, 241-light emitting diode, 25-condenser, 31-constant temperature pipeline, 311-circulating pump, 312-a flow control valve, 313-a heating pipe, 314-a heating wire, 231-a motor, 232-a telescopic column, 233-a rubber push sheet, 41-an objective lens, 42-a microscope tee joint, 43-an eyepiece, 44-an adapter, 45-a CCD industrial camera, 51-a lifting motor, 52-a lifting screw rod, 53-a lifting bracket, 54-a lifting slider, 55-a lifting track, 91-a PLC control device, 92-a display screen, 93-a camera, 94-a face recognition module and 95-a communication module.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to fig. 1 to 5 in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a constant temperature cell imaging system as shown in figures 1-5, which comprises a tank body 1, a constant temperature inner cavity 2, a constant temperature loop 3 and a trinocular biological image microscope 4, wherein:
a constant-temperature inner cavity 2 and a partition plate 11 are arranged inside the tank body 1, an inner cavity 12 is formed among the tank body 1, the constant-temperature inner cavity 2 and the partition plate 11, and the inner cavity 12 is filled with a liquid medium;
the bottom of the partition plate 11 is fixedly provided with a constant temperature loop 3, and the constant temperature loop 3 can keep the temperature inside the constant temperature inner cavity 2 controllable and can provide an environment with controllable temperature;
a glass slide 21 is arranged in the constant-temperature inner cavity 2, and a light source 24 is arranged below the glass slide 21;
a cell culture tank 22 and a shifting mechanism 23 capable of horizontally shifting the cell culture tank 22 are arranged above the glass slide 21, and different positions of the cell culture tank 22 can be subjected to microscopic imaging through the shifting mechanism 23, so that the defect that the conventional microscopic imaging lens is small in imaging can be overcome;
a trinocular biological image microscope 4 is arranged above the tank body 1, the trinocular biological image microscope 4 is fixedly connected with a lifting device 5 arranged behind the tank body 1 through a connecting pipe 6, and the cell culture process in the cell culture tank 22 can be observed through the trinocular biological image microscope 4;
the trinocular biological image microscope 4 is fixedly connected with a processing device 8 which can process images and signals through a connecting cable 7, and the processing device 8 is fixedly connected with a display 9;
a cell culture process;
the imaging device 4 is fixedly connected with a processing device 8 which can process images and signals through a connecting cable 7, and the processing device 8 is fixedly connected with a display 9;
a camera 93 is arranged on the display 9, and a face recognition module 94 and a communication module 95 are arranged inside the display 9;
the communication module 95 comprises a WiFi communication module and a mobile communication module;
the face recognition module 94 can authenticate the face information collected by the camera 93, the face recognition module 94 can take the face outline or iris information of a user as reference, an experimenter can manage the PLC control device 91 inside the display 9 by touching the display screen 92, the face information of a new user is recorded in the storage device in the PLC control device 91, invalid user information can be deleted, meanwhile, the working parameters of the PLC control device 91 can be adjusted by means of the display screen 92, faults during face recognition are eliminated, after the face recognition succeeds, the face recognition module 94 directly starts the display 9 to improve the operation convenience, when the face recognition is unsuccessful, the display 9 is in a dormant state.
But through communication module 95 remote control PLC controlling means 91 to open or close display screen 92, heating pipe 313, elevator motor 51, motor 231 and circulating pump 311 through PLC controlling means 91 operation, make mobile terminal such as accessible cell-phone and thing networking constant temperature cell imaging system communication connection, directly operate thing networking constant temperature cell imaging system through the cell-phone.
Specifically, the left side and the right side of the top of the tank body 1 are correspondingly provided with the tank door 13, the tank door 13 is provided with the tank door handle 131, one end of the tank door 13 is movably connected with the tank body 1 through the hinge column 14, the tank door 13 can rotate left and right relative to the tank body 1 through the hinge column 14, the tank door 13 on the left side and the right side is provided with the connecting ring 132, the connecting ring 132 correspondingly arranged on the left side and the right side can be combined into the hollow cylinder 133, the hollow cylinder 133 can accommodate the trinocular biological image microscope 4 to move up and down to enter and exit the tank body 1, the tank body 1 is provided with the sliding groove 15 capable of accommodating the connecting pipe 6 to lift, when the tank doors 13 on the left side and the right side are closed, the inside of the constant-temperature inner cavity 2 can be in a closed dark environment, the influence of other light rays on the trinocular biological image microscope 4 can be reduced, and when the tank doors on the left side While maintaining the physiological activity of the cells.
Specifically, constant temperature loop 3 includes constant temperature pipeline 31, constant temperature pipeline 31 is intake and is held through baffle 11 and set up in 11 left ends of baffle just constant temperature pipeline 31 goes out water end and runs through baffle 11 and set up in 11 right sides of baffle, be provided with circulating pump 311, flow control valve 312 and heating pipe 313 on constant temperature pipeline 31, heating pipe 313 is inside to be provided with the electric heating wire 314 who encircles constant temperature pipeline 31 outer wall, electric heating wire 314 utilizes electric energy transformation to become heat energy to this reaches the effect to the heating of liquid medium in the constant temperature pipeline 31.
Specifically, the shifting mechanism 23 includes a motor 231, a telescopic column 232 and a rubber push-piece 233, the motor 231 is disposed on the inner wall of the constant-temperature inner cavity 2, the motor 231 is disposed at four ends, front, rear, left and right, of the constant-temperature inner cavity 2, the output end of the motor 231 is correspondingly matched with the telescopic column 232, the other end of the telescopic column 232 is fixedly connected with the rubber push-piece 233, and the motor 231 can drive the telescopic column 232 and the rubber push-piece 233 to translate, so that the rubber push-piece 233 can move the cell culture tank 22 in a transverse and/or longitudinal manner, so that the cells in the cell culture tank 22 can be adjusted to corresponding positions and microscopic imaging can be completed.
Specifically, slide 21 inlays to be located inside 2 of constant temperature inner chamber body just slide 21 is transparent and can bear and place the transparent cell culture jar 22 that waits to cultivate the cell, slide 21 below is provided with light source 24 just light that light source 24 sent can be through the condensing lens focus that light source 24 top set up, and the inside light filter that can set up of condensing lens, and light after the focus can be permeable slide 21 shines cell in the cell culture jar 22, light source 24 is for corresponding the emitting diode 241 that slide 21 array set up, the accessible is adjusted emitting diode 241 on-off quantity and is reached and adjust the light luminance that light source 24 sent.
Specifically, the biological image microscope 4 bottom of trinocular is provided with objective 41, parallel arrangement each other between objective 41 and the cell culture jar 22, biological image microscope 4 of trinocular includes microscope tee bend 42 just be provided with eyepiece 43 and switching mouth 44 on the microscope tee bend 42, eyepiece 43 is 30 degrees inclination angle and sets up in microscope tee bend 42 the place ahead just microscope tee bend 42 top is provided with switching mouth 44, switching mouth 44 top is fixed and is provided with CCD industry camera 45, CCD industry camera 45 accessible connecting cable 7 and processing apparatus 8 electric connection, and the cell in the cell culture jar 22 becomes handstand, enlarged real image through objective 41, and this real image passes through eyepiece 43 again and generates upright, enlarged virtual image, enlarges into the handstand enlarged virtual image that can be observed by the people's eye through objective 41 and eyepiece 43, and CCD industry camera 45 accessible connecting cable 7 and the processing apparatus 8 fixed connection that can carry out image and signal processing just processing apparatus 8 fixed connection just The device 8 is fixedly connected to the display 9 so that the cell characteristic information can be displayed on the display 9, and the area of the image of the cell characteristic information viewed on the display 9 can be the same as the area viewed under the eyepiece 43.
Specifically, the lifting device 5 comprises a lifting motor 51, a lifting screw 52, a lifting bracket 53 and a lifting slider 54; the lifting screw 52 is vertically arranged, the bottom of the lifting screw is correspondingly matched and connected with an output shaft of the lifting motor 51 and can rotate under the driving of the output shaft of the lifting motor 51, the lifting slide block 54 is matched with the lifting screw 52 to form a threaded screw rod, the lifting slide block 54 is externally coated with a lifting support 53, the lifting support 53 and the lifting slide block 54 are both in a square cylinder structure, the lifting support 53 is provided with a lifting track 55 right facing the connecting pipe 6, the rotation of the lifting screw rod 52 can be correspondingly converted into the axial lifting motion of the lifting slide block 54 along the lifting screw rod 52, so that the trinocular biological image microscope 4 is driven to complete vertical lifting, and the trinocular biological image microscope 4 is enabled to complete the focusing on cells on the cell culture tank 22.
Specifically, the inner chamber 12 inner wall left and right sides corresponds and is provided with temperature sensor 121, can real-time detection state the real-time temperature of the inside liquid medium of inner chamber 12, inner chamber 12 outer wall spiral is around being provided with heating wire 122, and operating personnel can adjust how much to the power supply volume of heating wire 122 according to the demand to the temperature of control heating satisfies the demand that different temperatures were disinfected, and heating wire 122 spiral encircles inner chamber 12 outer wall setting, can reach required sterilizing temperature fast and sterilizing temperature can reach 250 degrees, inner chamber 12 inner wall is provided with sterilamp 123 just sterilamp 123 sets up in slide 21 top, and sterilamp 123 can kill the bacterium in the inner chamber 12 air.
A PLC control device 91 is arranged inside the display 9, and a display screen 92 is arranged on the display 9, the display screen 92, the PLC control device 91, the light source 24, the temperature sensor 121, the heating pipe 313, the lifting motor 51, the motor 231, the PH detector 222, the heating wire 122, and the ultraviolet germicidal lamp 123 are electrically connected, the PLC control device 91 has a storage function, the characteristic information of the cells can be stored and displayed on the display screen 92 when needed, and the PLC control device 91 can control the heating pipe 313 and the flow control valve 312 through the temperature sensor 121 to achieve the constant temperature of the liquid medium in the inner cavity 12, and when the PLC 91 detects that the PH value in the cell culture tank 22 exceeds the range through the PH detector 222, the PLC can display an image alarm through the display screen 92 to remind an operator.
The working principle is as follows: when the constant-temperature cell imaging system is used, firstly, cells are placed in a clean transparent cell culture tank 22 according to a specified method for culture, then a light source 24 arranged below a glass slide 21 is turned on, a PLC (programmable logic controller) 91 can control and adjust the light source 24 to emit uniform horizontal light and can control the on-off of the light source 24, the light emitted by the light source 24 can penetrate through the glass slide 21 and irradiate the cells in the cell culture tank 22, the light emitted by a light emitting diode 241 arranged in an array can penetrate through the glass slide 21 and illuminate the cells on the cell culture tank 22, the cells in the cell culture tank 22 form an inverted and amplified real image through an objective lens 41, the real image generates an upright and amplified virtual image through an eyepiece 43 and is amplified into an inverted and amplified virtual image which can be observed by human eyes through the objective lens 41 and the eyepiece lens 43, a lifting motor 51 arranged on a lifting device 5 can drive a lifting screw rod 52 to rotate so that a lifting slide block 54 moves up and down along the axial direction of the, thereby driving the trinocular biological image microscope 4 to complete vertical lifting, leading the trinocular biological image microscope 4 to complete the focusing of the cultured cells in the cell culture tank 22, leading the shifting mechanism 23 to drive the cell culture tank 22 to do two-dimensional motion along the horizontal direction, leading the shifting mechanism 23 to complete the vertical lifting of the trinocular biological image microscope 4, the relative position of the cell culture tank 22 plated with cells and the trinocular biological image microscope 4 can be adjusted, can enlarge the microscopic imaging range while improving the microscopic imaging accuracy, can observe the whole process of cell differentiation by a trinocular biological image microscope 4 while keeping the constant temperature of the cells, and the CCD industrial camera 45 can be fixedly connected with a processing device 8 which can process images and signals through a connecting cable 7, and the processing device 8 is fixedly connected with a display 9, so that cell characteristic information can be displayed on the display 9 to facilitate later consultation.
When the constant-temperature cell imaging system based on the Internet of things is used by an individual, individual cells are arranged in a clean transparent cell culture tank 22 according to a specified method, then a light source 24 arranged below a glass slide 21 can be turned on through a communication module 95 by using a mobile terminal such as a mobile phone, or the light source 24 arranged below the glass slide 21 can be turned on through a display screen 92, a PLC (programmable logic controller) 91 can control and adjust the light source 24 to emit uniform horizontal light and can control the light emitting diodes 241 to be turned on and off, the light emitted by the light source 24 can be focused through a condenser arranged above the light source 24, focused light can be irradiated to cells in the cell culture tank 22 through the glass slide 21, the light emitted by the light emitting diodes 241 arranged in an array can illuminate the cells on the cell culture tank 22 through the glass slide 21, and the mobile terminal such as the mobile phone can be used for controlling a lifting motor 51 arranged on a lifting device 5 through the communication module 95 to drive a lifting screw 52 The block 54 moves up and down along the axial direction of the lifting screw 52, thereby driving the imaging device 4 to complete vertical lifting, enabling the imaging device 4 to complete focusing on cells on the cell culture tank 22, and enabling mobile terminals such as mobile phones and the like to be capable of driving the cell culture tank 22 to do two-dimensional motion along the horizontal direction through the shifting mechanism 23, enabling the relative position of the cell culture tank 22 paved with the cells and the imaging device 4 to be adjusted through the shifting mechanism 23, being capable of expanding the micro-imaging range while improving the micro-imaging accuracy, enabling the cells to be kept at a constant temperature and simultaneously being capable of observing the whole process of cell differentiation culture through the imaging device 4, observing a virtual inverted and amplified image of the cells through the ocular lens 43, storing characteristic information of the cells through the CCD industrial camera 45, the processing device 8 and the display 9, and displaying the characteristic information on a display screen communicated with the mobile terminals such as mobile phones and the like when needed, the later-stage consultation is facilitated, the state of the individual cell can be watched at any time through the mobile phone, and the whole process of cell differentiation culture can be observed through the mobile phone.
When the constant-temperature cell imaging system based on the Internet of things is used in a hospital, the camera 93 arranged on the display 9 and the face recognition module 94 arranged in the display 9 can be used for carrying out face recognition on an operator, when the face recognition is successful, the face recognition module 94 can directly start the display 9, the operation convenience is improved, the cells are arranged in the clean transparent cell culture tank 22 according to a specified method,
then, a mobile terminal such as a mobile phone can be used to turn on the light source 24 arranged below the glass slide 21 through the communication module 95, or the light source 24 arranged below the glass slide 21 can be turned on through the display screen 92, the PLC control device 91 can control and adjust the light source 24 to emit uniform horizontal light and can control the on/off of the light emitting diodes 241, the light emitted by the light source 24 can be focused by the condenser lens arranged above the light source 24, the focused light can be irradiated to the cells in the cell culture tank 22 through the glass slide 21, the light emitted by the light emitting diodes 241 arranged in the array can be irradiated to the cells on the cell culture tank 22 through the glass slide 21, and the mobile terminal such as a mobile phone can be used to control the lifting motor 51 arranged on the lifting device 5 through the communication module 95 to drive the lifting screw 52 to rotate so that the lifting slider 54 can move up and down along the axial direction of the lifting screw 52, thereby driving, the imaging device 4 can complete the focusing of the cells on the cell culture tank 22, and can drive the cell culture tank 22 to move horizontally by using mobile terminals such as mobile phones and the like through the shifting mechanism 23 of the communication module 95, the shifting mechanism 23 can adjust the relative positions of the cell culture tank 22 paved with the cells and the imaging device 4, can enlarge the microscopic imaging range while improving the microscopic imaging accuracy, can observe the whole process of cell differentiation through the imaging device 4 while keeping the cells at a constant temperature, can observe the inverted and amplified virtual images of the cells through the ocular lens 43, can store the characteristic information of the cells through the CCD industrial camera 45, the processing device 8 and the display 9, can be displayed on a display screen communicated with the mobile terminals such as the mobile phones and the like when needed, is convenient for later-stage consultation, and can observe the cell states through the mobile phones at any time, and the whole process of the culture of cell differentiation can be observed through the mobile phone, and the PLC control device 91 arranged in the display 9 can supervise the operator in real time through the camera 93 arranged on the display 9, so that the operation accident is prevented, and the cell culture state in the cell culture tank 22 can be monitored in real time through mobile terminals such as the mobile phone.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.
Claims (10)
1. The utility model provides a constant temperature cell imaging system, includes cell body (1), cavity (2) in the constant temperature, constant temperature return circuit (3), trinocular biological image microscope (4), its characterized in that:
a constant-temperature inner cavity (2) and a partition plate (11) are arranged in the tank body (1), an inner cavity (12) is formed among the tank body (1), the constant-temperature inner cavity (2) and the partition plate (11), and the inner cavity (12) is filled with a liquid medium;
a constant temperature loop (3) is fixedly arranged at the bottom of the partition plate (11);
a glass slide (21) is arranged in the constant-temperature inner cavity (2), and a light source (24) is arranged below the glass slide (21);
a cell culture tank (22) and a displacement mechanism (23) capable of horizontally displacing the cell culture tank (22) are arranged above the glass slide (21);
a trinocular biological image microscope (4) is arranged above the tank body (1), and the trinocular biological image microscope (4) is fixedly connected with a lifting device (5) arranged behind the tank body (1) through a connecting pipe (6);
the trinocular biological image microscope (4) can be fixedly connected with a processing device (8) capable of processing images and signals through a connecting cable (7), and the processing device (8) is fixedly connected with a display (9);
a camera (93) is arranged on the display (9), and a face recognition module (94) and a communication module (95) are arranged in the display (9);
the communication module (95) comprises a WiFi communication module and a mobile communication module;
the face recognition module (94) can authenticate the face information collected by the camera.
2. A system for isothermal cell imaging according to claim 1, characterized by: the utility model discloses a three-purpose biological image microscope, including cell body (1), cell body (1) top left and right sides correspondence is provided with slot door (13) just be provided with slot door handle (131) on slot door (13), hinge post (14) and cell body (1) swing joint just are passed through to slot door (13) one end groove door (13) accessible hinge post (14) for cell body (1) horizontal rotation, be provided with go-between (132) just on left and right sides slot door (13) the go-between (132) that the left and right sides corresponds the setting can make up into hollow cylinder (133), hollow cylinder (133) can hold three-mesh biological image microscope (4) translation business turn over cell body (1) from top to bottom, be provided with spout (15) that can hold connecting pipe (6) and go up and down on cell body.
3. A system for isothermal cell imaging according to claim 1, characterized by: constant temperature loop (3) are including constant temperature pipeline (31), constant temperature pipeline (31) are intake and are held to run through baffle (11) and set up in baffle (11) left end just constant temperature pipeline (31) play water end runs through baffle (11) and set up in baffle (11) right-hand member side, be provided with circulating pump (311), flow control valve (312) and heating pipe (313) on constant temperature pipeline (31), heating pipe (313) inside is provided with electric heating wire (314) around constant temperature pipeline (31) outer wall.
4. A system for isothermal cell imaging according to claim 3, characterized by: the shifting mechanism (23) comprises a motor (231), a telescopic column (232) and a rubber push sheet (233), the motor (231) is arranged on the inner wall of the constant-temperature inner cavity (2), the motor (231) is arranged on the front, back, left and right ends of the constant-temperature inner cavity (2), the output end of the motor (231) is correspondingly matched with the telescopic column (232), the other end of the telescopic column (232) is fixedly connected with the rubber push sheet (233), and the motor (231) can drive the telescopic column (232) and the rubber push sheet (233) to translate, so that the rubber push sheet (233) can transversely and/or longitudinally move the cell culture tank (22), and cells on the cell culture tank (22) can be adjusted to corresponding positions and microscopic imaging can be completed.
5. A system for isothermal cell imaging according to claim 1, characterized by: slide glass (21) inlay and locate cavity (2) inside just in the constant temperature slide glass (21) are transparent and can bear and place transparent cell culture jar (22) that await the culture cell, slide glass (21) below is provided with light source (24) just light that light source (24) sent can be focused through condensing lens (25) that light source (24) top set up, and light after the focus is permeable slide glass (21) shine cell in cell culture jar (22), light source (24) are for corresponding emitting diode (241) that slide glass (21) array set up.
6. A system for isothermal cell imaging according to claim 1, characterized by: the utility model discloses a biological image microscope of trinocular, including microscope tee bend (42), switching mouth (44), eyepiece (43) and switching mouth (44) are provided with on microscope tee bend (42), eyepiece (43) are certain inclination and set up in microscope tee bend (42) the place ahead just microscope tee bend (42) top is provided with switching mouth (44), switching mouth (44) top is fixed and is provided with CCD industry camera (45), CCD industry camera (45) accessible connecting cable (7) and processing apparatus (8) electric connection between trinocular biological image microscope (4) bottom, mutual parallel arrangement between objective (41) and cell culture jar (22), trinocular biological image microscope (4) include microscope tee bend (42) just be provided with eyepiece (43) and switching mouth (44) on microscope tee bend (42).
7. A system for isothermal cell imaging according to claim 1, characterized by: the lifting device (5) comprises a lifting motor (51), a lifting screw rod (52), a lifting bracket (53) and a lifting slider (54); the lifting screw rod (52) is vertically arranged, the bottom of the lifting screw rod is correspondingly matched and connected with the output shaft of the lifting motor (51) and can rotate under the driving of the output shaft of the lifting motor (51), and the lifting slide block (54) is matched with the lifting screw rod (52) to form a threaded screw rod, the lifting support (53) is coated outside the lifting slide block (54), the lifting support (53) and the lifting slide block (54) are both of square column structures, a lifting track (55) is arranged at the position of the lifting bracket (53) opposite to the connecting pipe (6), the rotation of the lifting screw rod (52) can be correspondingly converted into the lifting movement of the lifting slide block (54) along the axial direction of the lifting screw rod (52), thereby driving the three-mesh biological image microscope (4) to complete vertical lifting, and enabling the three-mesh biological image microscope (4) to complete focusing on cells in the cell culture tank (22).
8. A system for isothermal cell imaging according to claim 1, characterized by: the temperature sensor (121) is correspondingly arranged on the left side and the right side of the inner wall of the constant-temperature inner cavity (2), the heating wire (122) is spirally arranged on the outer wall of the inner cavity (12) in a surrounding mode, the inner wall of the inner cavity (12) is provided with the ultraviolet germicidal lamp (123), and the ultraviolet germicidal lamp (123) is arranged above the glass slide (21).
9. A system for isothermal cell imaging according to claim 1, characterized by: the cell culture tank (22) is of a hollow tank structure with an outer square and an inner circle, and a stirrer (221) and a PH detector (222) are arranged inside the cell culture tank (22).
10. A system for isothermal cell imaging according to claim 1, characterized by: the LED lamp is characterized in that a PLC control device (91) is arranged inside the display (9), a display screen (92) is arranged on the display (9), and the display screen (92), the PLC control device (91), the light source (24), the temperature sensor (121), the heating pipe (313), the lifting motor (51), the motor (231), the PH detector (222), the heating wire (122) and the ultraviolet germicidal lamp (123) are electrically connected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911113196.4A CN111103284A (en) | 2019-11-14 | 2019-11-14 | Constant temperature cell imaging system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911113196.4A CN111103284A (en) | 2019-11-14 | 2019-11-14 | Constant temperature cell imaging system |
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| Publication Number | Publication Date |
|---|---|
| CN111103284A true CN111103284A (en) | 2020-05-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911113196.4A Withdrawn CN111103284A (en) | 2019-11-14 | 2019-11-14 | Constant temperature cell imaging system |
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| CN (1) | CN111103284A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111592986A (en) * | 2020-05-27 | 2020-08-28 | 南京铭声通电子科技有限公司 | Intelligent culture and remote observation culture device and method |
| CN111592984A (en) * | 2020-06-16 | 2020-08-28 | 赵宇航 | Multifunctional cell detector |
| CN114136968A (en) * | 2021-11-25 | 2022-03-04 | 杭州艾名医学科技有限公司 | Three-dimensional cell and organoid in-vitro growth noninvasive imaging system |
-
2019
- 2019-11-14 CN CN201911113196.4A patent/CN111103284A/en not_active Withdrawn
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111592986A (en) * | 2020-05-27 | 2020-08-28 | 南京铭声通电子科技有限公司 | Intelligent culture and remote observation culture device and method |
| CN111592986B (en) * | 2020-05-27 | 2024-03-19 | 南京兰伯艾克斯生物科技有限公司 | Intelligent culture and remote observation culture device and method |
| CN111592984A (en) * | 2020-06-16 | 2020-08-28 | 赵宇航 | Multifunctional cell detector |
| CN114136968A (en) * | 2021-11-25 | 2022-03-04 | 杭州艾名医学科技有限公司 | Three-dimensional cell and organoid in-vitro growth noninvasive imaging system |
| CN114136968B (en) * | 2021-11-25 | 2024-03-26 | 杭州艾名医学科技有限公司 | Three-dimensional cell and organoid in-vitro growth noninvasive imaging system |
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