CN114136968A - Three-dimensional cell and organoid in-vitro growth noninvasive imaging system - Google Patents
Three-dimensional cell and organoid in-vitro growth noninvasive imaging system Download PDFInfo
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- CN114136968A CN114136968A CN202111412606.2A CN202111412606A CN114136968A CN 114136968 A CN114136968 A CN 114136968A CN 202111412606 A CN202111412606 A CN 202111412606A CN 114136968 A CN114136968 A CN 114136968A
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- 238000003384 imaging method Methods 0.000 title claims abstract description 71
- 210000002220 organoid Anatomy 0.000 title claims abstract description 30
- 238000000338 in vitro Methods 0.000 title claims abstract description 28
- 230000012010 growth Effects 0.000 title claims abstract description 17
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 3
- 238000009434 installation Methods 0.000 claims description 4
- 230000006978 adaptation Effects 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims description 2
- 238000012544 monitoring process Methods 0.000 abstract description 6
- 210000000056 organ Anatomy 0.000 abstract description 4
- 238000005096 rolling process Methods 0.000 abstract description 3
- 210000001519 tissue Anatomy 0.000 abstract description 3
- 241001465754 Metazoa Species 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000007306 turnover Effects 0.000 description 2
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
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- 230000008467 tissue growth Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
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Abstract
The invention relates to the technical field of cell and tissue culture, in particular to a three-dimensional cell and organoid in-vitro growth noninvasive imaging system; including the formation of image box, transparent base, the culture dish, the light source, first imaging element and second imaging element, first imaging element includes the rolling disc, rotating assembly, and a support, first camera and second camera, it carries out image acquisition to start second imaging element, utilize first camera to carry out image acquisition to the center department of the bottom of culture dish, start rotating assembly, it rotates on the swivel becket to drive the rolling disc through driven tooth portion, make the second camera rotate along the circumference line of the bottom of culture dish, thereby utilize the second camera to carry out omnidirectional image information acquisition to the bottom of culture dish, monitoring three-dimensional cell and the external condition of cultivateing of class organ more comprehensively.
Description
Technical Field
The invention relates to the technical field of cell and tissue culture, in particular to a three-dimensional cell and organoid in-vitro growth noninvasive imaging system.
Background
In the research of tumor and other tissue growth, the in vitro culture of organoid plays an important role, and can replace the animal transplantation method such as PDX, etc., to carry out the in vitro research with convenience, rapidness, low cost and no animal ethical risk. The tumor or other tissues are scattered into organoids, or three-dimensional cell masses which grow by unicellular clone are cultured in vitro, and the growth capacity of a certain cell strain or a specific organoid under a specific environment can be reflected by replacing in-vivo experiments of animals.
When a culture dish is used for culturing three-dimensional cells and organoids in vitro, an image pickup mechanism is often needed to collect image information, so that the culture conditions of the three-dimensional cells and the organoids are monitored.
However, when the existing three-dimensional cells and organoids are cultured in vitro, the camera shooting mechanism is often fixedly arranged at the top of the culture dish, so that the image information is acquired, the acquisition range is small, and the culture condition of the three-dimensional cells and organoids cannot be comprehensively monitored.
Disclosure of Invention
The invention aims to provide a noninvasive imaging system for in vitro growth of three-dimensional cells and organoids, which solves the problems that in the prior art, when the existing three-dimensional cells and organoids are cultured in vitro, a camera mechanism is often fixedly arranged at the top of a culture dish, so that image information is acquired, the acquisition range is small, and the culture condition of the three-dimensional cells and organoids cannot be comprehensively monitored.
In order to achieve the above object, the present invention provides a three-dimensional cell and organoid in vitro growth noninvasive imaging system, which includes an imaging box, a transparent base, a culture dish, a light source, a first imaging unit and a second imaging unit, wherein the transparent base is disposed at the bottom of the inside of the imaging box, the culture dish is disposed above the transparent base, the first imaging unit is disposed inside the transparent base, and the second imaging unit and the light source are disposed at the top of the inside of the imaging box;
the first imaging unit comprises a rotating disc, a rotating assembly, a support, a first camera and a second camera, a rotating ring is arranged on the side wall of the inside of the transparent base, the rotating disc is movably connected with the rotating ring, a through hole is formed in the center of the rotating disc, the rotating assembly and the support are arranged at the bottom of the inside of the transparent base, the first camera is arranged at one end, away from the bottom of the transparent base, of the support, the first camera is located inside the through hole, the output end of the first camera corresponds to the center of the bottom of the culture dish, a driven tooth part is arranged at one end, close to the bottom of the transparent base, of the rotating disc, the second camera is arranged at one end, close to the culture dish, of the rotating disc, and the output end of the second circumferential camera corresponds to the bottom line of the culture dish, the output end of the rotating assembly corresponds to the driven tooth part.
Utilize the light source is in the imaging box the culture dish throws light on, starts the second imaging unit is right image acquisition is carried out at the top of culture dish, utilizes first camera is to carry out image acquisition in the center department of the bottom of culture dish, starts rotating assembly, through from the drive of toothed gearing the rolling disc is in rotate on the swivel becket, makes the second camera is followed the circumference line of the bottom of culture dish rotates, thereby utilizes the second camera is to carry out omnidirectional image information acquisition in the bottom of culture dish, monitoring three-dimensional cell and the external condition of cultivateing of class organ more comprehensively.
The rotating assembly comprises a first servo motor, a first gear reduction box and an output gear, the first servo motor is detachably connected with the bottom of the interior of the transparent base, the first gear reduction box is arranged at the output end of the first servo motor, the output gear is arranged at the output end of the first gear reduction box, and the output gear is meshed with the driven gear.
And starting the first servo motor, driving the output gear to rotate after the output rotating speed of the first servo motor is reduced by using the first gear reduction box, and driving the rotating ring to rotate as the output gear is meshed with the driven tooth part.
Wherein, the second imaging unit is including promoting subassembly, sliding seat, support frame, mount pad and third camera, the top of the inside of formation of image box be provided with the slide rail with promote the subassembly, the sliding seat with slide rail sliding connection, the sliding seat is close to the one end of slide rail is provided with the connecting plate, the sliding seat is kept away from the one end of sliding seat is provided with the support frame, the support frame is kept away from the one end of sliding seat is provided with the mount pad, be provided with on the mount pad the third camera, the output of third camera with the top of culture dish is corresponding, the output that promotes the subassembly with the connecting plate is corresponding.
The pushing assembly is started, the sliding seat is driven to move on the sliding rail through the connecting plate, so that the shooting range of the third camera is adjusted, and the in-vitro culture condition of the three-dimensional cells and the organoids can be monitored more comprehensively.
The pushing assembly comprises a second servo motor, a screw rod and a screw rod sleeve, the second servo motor is detachably connected with the top of the inside of the imaging box body, the output end of the second servo motor is provided with the screw rod, the screw rod sleeve is arranged on the screw rod, and the screw rod sleeve is detachably connected with the connecting plate.
And starting the second servo motor to drive the screw rod to rotate, and driving the sliding seat to translate on the sliding rail due to the fact that the screw rod sleeve is detachably connected with the connecting plate.
The pushing assembly further comprises a supporting piece, one end of the supporting piece is detachably connected with the top of the inside of the imaging box body, the other end of the supporting piece is sleeved on the outside of the screw rod, and the supporting piece is located at one end, far away from the second servo motor, of the screw rod.
One end of the screw rod, which is far away from the second servo motor, is provided with the support piece, and the screw rod is supported by the support piece, so that the phenomenon that the screw rod is damaged due to overlarge stress at the joint of the screw rod and the second servo motor is avoided.
Wherein, the support frame includes electric putter, sleeve pipe, third servo motor, output shaft and mounting panel, electric putter's installation end with the connection is dismantled to the sliding seat, electric putter's output is provided with the sleeve pipe, sheathed tube inside is provided with third servo motor, the one end of mounting panel with sleeve pipe swing joint, the other end of mounting panel is provided with the mounting panel, third servo motor's output is provided with the output shaft, be provided with the fixture block on the output shaft, the mounting panel is close to sheathed tube one side is provided with the draw-in groove, the fixture block with draw-in groove looks adaptation.
The support frame by electric putter the sleeve pipe third servo motor the output shaft with the mounting panel constitutes, utilizes electric putter can adjust the height of mount pad to adjust third camera with distance between the culture dish, through third servo motor drives the output shaft rotates, thereby drives the mounting panel rotates, makes the mount pad is in circular motion is done at the top of culture dish, thereby enlarges the shooting scope of third camera, monitoring three-dimensional cell and the external condition of cultivateing of class organ that can be more comprehensive.
The invention relates to a three-dimensional cell and organoid in-vitro growth noninvasive imaging system which comprises an imaging box body, a transparent base, a culture dish, a light source, a first imaging unit and a second imaging unit, wherein the second imaging unit and the light source are arranged at the top inside the imaging box body, the first imaging unit comprises a rotating disc, a rotating assembly, a bracket, a first camera and a second camera, the culture dish in the imaging box body is illuminated by the light source, the second imaging unit is started to acquire images at the top of the culture dish, the first camera is used to acquire images at the center of the bottom of the culture dish, the rotating assembly is started, the rotating disc is driven to rotate on the rotating ring through a driven tooth part, so that the second camera rotates along the circumferential line of the bottom of the culture dish, therefore, the second camera is used for collecting the image information of the bottom of the culture dish in all directions, and the in-vitro culture condition of the three-dimensional cells and the organoid can be monitored more comprehensively.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a front view of a three-dimensional in vitro cell and organoid growth noninvasive imaging system provided by the present invention.
Fig. 2 is a sectional view of the internal structure taken along line a-a of fig. 1 according to the present invention.
Fig. 3 is a sectional view of the inner structure of fig. 1 taken along line B-B according to the present invention.
Fig. 4 is a schematic structural diagram of a first imaging unit provided by the present invention.
Fig. 5 is a schematic structural diagram of a second imaging unit provided by the present invention.
Fig. 6 is an enlarged view of a portion of the structure of fig. 5 a according to the present invention.
1-imaging box body, 2-transparent base, 3-culture dish, 4-light source, 5-first imaging unit, 51-rotating disc, 52-rotating assembly, 521-first servo motor, 522-first gear reduction box, 523-output gear, 53-bracket, 54-first camera, 55-second camera, 56-rotating ring, 57-through hole, 58-driven tooth part, 6-second imaging unit, 61-pushing assembly, 611-second servo motor, 612-screw rod, 613-screw rod sleeve, 614-supporting piece, 62-sliding seat, 63-supporting frame, 631-electric push rod, 632-sleeve pipe, 633-third servo motor, 634-output shaft, 635-mounting plate, 6351-plate body, 6352-turnover frame, 636-fixture block, 637-fixture groove, 638-turnover assembly, 6381-third servo motor, 6382-second gear reduction box, 6383-coupler, 64-mounting seat, 641-rotating shaft, 642-connecting body, 643-mounting block, 65-third camera, 66-sliding rail and 67-connecting plate.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
In the description of the present invention, it is to be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships illustrated in the drawings, and are used merely for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, are not to be construed as limiting the present invention. Further, in the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Referring to fig. 1 to 6, the present invention provides a three-dimensional cell and organoid in vitro growth noninvasive imaging system, which includes an imaging box 1, a transparent base 2, a culture dish 3, a light source 4, a first imaging unit 5 and a second imaging unit 6, wherein the transparent base 2 is disposed at the bottom of the interior of the imaging box 1, the culture dish 3 is disposed above the transparent base 2, the first imaging unit 5 is disposed in the interior of the transparent base 2, and the second imaging unit 6 and the light source 4 are disposed at the top of the interior of the imaging box 1;
the first imaging unit 5 comprises a rotating disc 51, a rotating assembly 52, a bracket 53, a first camera 54 and a second camera 55, a rotating ring 56 is arranged on the side wall of the inside of the transparent base 2, the rotating disc 51 is movably connected with the rotating ring 56, a through hole 57 is arranged at the center of the rotating disc 51, the rotating assembly 52 and the bracket 53 are arranged at the bottom of the inside of the transparent base 2, the first camera 54 is arranged at one end of the bracket 53 far away from the bottom of the transparent base 2, the first camera 54 is positioned inside the through hole 57, the output end of the first camera 54 corresponds to the center of the bottom of the culture dish 3, a driven tooth part 58 is arranged at one end of the rotating disc 51 close to the bottom of the transparent base 2, and the second camera 55 is arranged at one end of the rotating disc 51 close to the culture dish 3, the output end of the second camera 55 corresponds to the circumferential line of the bottom of the culture dish 3, and the output end of the rotating assembly 52 corresponds to the driven tooth part 58.
In this embodiment, the light source 4 is used to illuminate the culture dish 3 in the imaging box 1, the second imaging unit 6 is started to collect images of the top of the culture dish 3, the first camera 54 is used to collect images of the center of the bottom of the culture dish 3, the rotating assembly 52 is started, the driven tooth part 58 drives the rotating disc 51 to rotate on the rotating ring 56, so that the second camera 55 rotates along the circumferential line of the bottom of the culture dish 3, the second camera 55 is used to collect all-around image information of the bottom of the culture dish 3, and the in-vitro culture condition of three-dimensional cells and organs can be monitored more comprehensively.
Further, the rotating assembly 52 includes a first servo motor 521, a first gear reduction box 522 and an output gear 523, the first servo motor 521 is detachably connected to the bottom of the inside of the transparent base 2, the output end of the first servo motor 521 is provided with the first gear reduction box 522, the output end of the first gear reduction box 522 is provided with the output gear 523, and the output gear 523 is engaged with the driven gear.
In this embodiment, the first servo motor 521 is started, the first gear reduction box 522 is used to slow down the output rotation speed of the first servo motor 521, and then the output gear 523 is driven to rotate, and the output gear 523 is meshed with the driven tooth part 58, so that the rotating ring 56 is driven to rotate.
Further, the second imaging unit 6 includes a pushing assembly 61, a sliding seat 62, a supporting frame 63, a mounting seat 64, and a third camera 65, a sliding rail 66 and the pushing assembly 61 are disposed on the top of the inside of the imaging box 1, the sliding seat 62 is slidably connected to the sliding rail 66, a connecting plate 67 is disposed at one end of the sliding seat 62 close to the sliding rail 66, the supporting frame 63 is disposed at one end of the sliding seat 62 far away from the sliding seat 62, the mounting seat 64 is disposed at one end of the supporting frame 63 far away from the sliding seat 62, the third camera 65 is disposed on the mounting seat 64, an output end of the third camera 65 corresponds to the top of the culture dish 3, an output end of the pushing assembly 61 corresponds to the connecting plate 67, the pushing assembly 61 includes a second servo motor 611, a lead screw 612, and a lead screw sleeve 613, the second servo motor 611 is detachably connected to the top of the inside of the imaging box 1, the output end of the second servo motor 611 is provided with the lead screw 612, the lead screw 612 is provided with the lead screw sleeve 613, and the lead screw sleeve 613 is detachably connected to the connecting plate 67.
In this embodiment, the second servo motor 611 is started to drive the screw rod 612 to rotate, and the screw rod sleeve 613 is detachably connected to the connecting plate 67, so as to drive the sliding seat 62 to translate on the sliding rail 66, thereby adjusting the shooting range of the third camera 65, and more comprehensively monitoring the in-vitro culture condition of three-dimensional cells and organoids.
Further, the pushing assembly 61 further includes a supporting member 614, one end of the supporting member 614 is detachably connected to the top of the inside of the imaging box 1, the other end of the supporting member 614 is sleeved on the outside of the screw rod 612, and the supporting member 614 is located at one end of the screw rod 612 far away from the second servo motor 611.
In this embodiment, the support 614 is disposed at an end of the screw rod 612 far away from the second servo motor 611, and the support 614 is used to support the screw rod 612, so as to avoid the screw rod 612 from being damaged due to an excessive force applied to a connection portion between the screw rod 612 and the second servo motor 611.
Further, the support frame 63 includes an electric push rod 631, a sleeve 632, a third servo motor 633, an output shaft 634 and a mounting plate 635, the mounting end of the electric push rod 631 is detachably connected to the sliding seat 62, the output end of the electric push rod 631 is provided with the sleeve 632, the third servo motor 633 is arranged inside the sleeve 632, one end of the mounting plate 635 is movably connected to the sleeve 632, the other end of the mounting plate 635 is provided with the mounting seat 64, the output end of the third servo motor 633 is provided with the output shaft 634, a clamping block 636 is arranged on the output shaft 634, a clamping groove 637 is arranged on one side, close to the sleeve 632, of the mounting plate 635, and the clamping block 636 is matched with the clamping groove 637.
In this embodiment, the supporting frame 63 is composed of the electric push rod 631, the sleeve 632, the third servo motor 633, the output shaft 634 and the mounting plate 635, the height of the mounting seat 64 can be adjusted by the electric push rod 631, so as to adjust the distance between the third camera 65 and the culture dish 3, the output shaft 634 is driven to rotate by the third servo motor 633, so as to drive the mounting plate 635 to rotate, so that the mounting seat 64 performs a circular motion at the top of the culture dish 3, thereby enlarging the shooting range of the third camera 65, and more comprehensively monitoring the in vitro culture condition of the three-dimensional cells and the organoids.
Further, the mounting plate 635 includes a plate body 6351, a turning frame 6352 and a turning assembly 638, the plate body 6351 is movably connected to the casing 632, the turning frame 6352 is detachably connected to the plate body 6351, and is located at an end of the plate body 6351 far away from the casing 632, the mounting seat 64 is movably connected to the turning frame 6352, the turning assembly 638 is disposed on the plate body 6351, and an output end of the turning assembly 638 corresponds to the mounting seat 64.
In this embodiment, the turning assembly 638 is started to drive the installation seat 64 to turn over on the turning frame 6352, so as to adjust the inclination angle of the installation seat 64, and further adjust the shooting angle of the third camera 65, thereby more comprehensively monitoring the in vitro culture condition of the three-dimensional cells and the organoids.
Further, the mounting base 64 includes a rotating shaft 641, a connecting body 642 and a mounting block 643, wherein one end of the connecting body 642 is fixedly connected to the rotating shaft 641, the other end of the connecting body 642 is fixedly connected to the mounting block 643, the third camera 65 is disposed on the mounting block 643, and the output end of the turning assembly 638 corresponds to the rotating shaft 641.
In this embodiment, the turning assembly 638 is activated to rotate the rotating shaft 641, so as to adjust the inclination angle of the mounting block 643, and the rotating shaft 641 and the mounting block 643 are both fixedly connected to the connecting body 642, and are manufactured by using an integral molding technique, so that the structure is more stable.
Further, the turnover assembly 638 includes a fourth servo motor 6381, a second gear reduction box 6382 and a coupler 6383, the fourth servo motor 6381 is detachably connected to the plate 6351, an output end of the fourth servo motor 6381 is provided with the second gear reduction box 6382, an output end of the second gear reduction box 6382 is provided with the coupler 6383, and the coupler 6383 corresponds to the rotating shaft 641.
In this embodiment, the fourth servo motor 6381 is started, and the output rotation speed of the fourth servo motor 6381 is reduced by the second gear reduction box 6382, and then the rotating shaft 641 is driven to rotate by the coupling 6383.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (6)
1. A three-dimensional cell and organoid in vitro growth noninvasive imaging system is characterized in that,
the non-invasive imaging system for in vitro growth of the three-dimensional cells and the organoids comprises an imaging box body, a transparent base, a culture dish, a light source, a first imaging unit and a second imaging unit, wherein the transparent base is arranged at the bottom inside the imaging box body, the culture dish is arranged above the transparent base, the first imaging unit is arranged inside the transparent base, and the second imaging unit and the light source are arranged at the top inside the imaging box body;
the first imaging unit comprises a rotating disc, a rotating assembly, a support, a first camera and a second camera, a rotating ring is arranged on the side wall of the inside of the transparent base, the rotating disc is movably connected with the rotating ring, a through hole is formed in the center of the rotating disc, the rotating assembly and the support are arranged at the bottom of the inside of the transparent base, the first camera is arranged at one end, away from the bottom of the transparent base, of the support, the first camera is located inside the through hole, the output end of the first camera corresponds to the center of the bottom of the culture dish, a driven tooth part is arranged at one end, close to the bottom of the transparent base, of the rotating disc, the second camera is arranged at one end, close to the culture dish, of the rotating disc, and the output end of the second circumferential camera corresponds to the bottom line of the culture dish, the output end of the rotating assembly corresponds to the driven tooth part.
2. The three-dimensional cell and organoid in vitro growth non-invasive imaging system according to claim 1,
the rotating assembly comprises a first servo motor, a first gear reduction box and an output gear, the first servo motor is detachably connected with the bottom of the interior of the transparent base, the first gear reduction box is arranged at the output end of the first servo motor, the output gear is arranged at the output end of the first gear reduction box, and the output gear is meshed with the driven gear.
3. The three-dimensional cell and organoid in vitro growth non-invasive imaging system according to claim 1,
the second imaging unit is including promoting subassembly, sliding seat, support frame, mount pad and third camera, the top of the inside of formation of image box be provided with the slide rail with promote the subassembly, the sliding seat with slide rail sliding connection, the sliding seat is close to the one end of slide rail is provided with the connecting plate, the sliding seat is kept away from the one end of sliding seat is provided with the support frame, the support frame is kept away from the one end of sliding seat is provided with the mount pad, be provided with on the mount pad the third camera, the output of third camera with the top of culture dish is corresponding, the output that promotes the subassembly with the connecting plate is corresponding.
4. The three-dimensional cell and organoid in vitro growth non-invasive imaging system according to claim 3,
the pushing assembly comprises a second servo motor, a screw rod and a screw rod sleeve, the second servo motor is detachably connected with the top of the inside of the imaging box body, the output end of the second servo motor is provided with the screw rod, the screw rod sleeve is arranged on the screw rod, and the screw rod sleeve is detachably connected with the connecting plate.
5. The three-dimensional cell and organoid in vitro growth non-invasive imaging system according to claim 4,
the pushing assembly further comprises a supporting piece, one end of the supporting piece is detachably connected with the top of the interior of the imaging box body, the other end of the supporting piece is sleeved on the outer portion of the screw rod, and the supporting piece is located at one end, far away from the screw rod, of the second servo motor.
6. The three-dimensional cell and organoid in vitro growth non-invasive imaging system according to claim 3,
the support frame includes electric putter, sleeve pipe, third servo motor, output shaft and mounting panel, electric putter's installation end with the connection is dismantled to the sliding seat, electric putter's output is provided with the sleeve pipe, sheathed tube inside is provided with third servo motor, the one end of mounting panel with sleeve pipe swing joint, the other end of mounting panel is provided with the mounting panel, third servo motor's output is provided with the output shaft, be provided with the fixture block on the output shaft, the mounting panel is close to sheathed tube one side is provided with the draw-in groove, the fixture block with draw-in groove looks adaptation.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115895831A (en) * | 2022-11-15 | 2023-04-04 | 江苏春江生物科技有限公司 | Fermented Chinese herbal medicine blood pressure reducing preparation for pacific people and preparation device |
| CN115895831B (en) * | 2022-11-15 | 2023-09-19 | 江苏春江生物科技有限公司 | Fermented Chinese herbal medicine antihypertensive preparation and preparation device thereof |
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