CN206858582U - A kind of fermentation tank for cultivating vegetable material callus - Google Patents

A kind of fermentation tank for cultivating vegetable material callus Download PDF

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Publication number
CN206858582U
CN206858582U CN201720662405.0U CN201720662405U CN206858582U CN 206858582 U CN206858582 U CN 206858582U CN 201720662405 U CN201720662405 U CN 201720662405U CN 206858582 U CN206858582 U CN 206858582U
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CN
China
Prior art keywords
fixedly installed
fermentation tank
vegetable material
callus
motor
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Expired - Fee Related
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CN201720662405.0U
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Chinese (zh)
Inventor
张克中
李建
李建一
崔金腾
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Beijing University of Agriculture
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Beijing University of Agriculture
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Priority to CN201720662405.0U priority Critical patent/CN206858582U/en
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Publication of CN206858582U publication Critical patent/CN206858582U/en
Expired - Fee Related legal-status Critical Current
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Abstract

The utility model discloses a kind of fermentation tank for cultivating vegetable material callus, one end is fixedly installed PH electrodes in the middle part of the fermentation tank, the PH electrodes lower end thereof is fixedly installed liquid insulating layer, the liquid insulating layer lower end thereof is fixedly installed bearing, the cooling water inlet left side central portion is fixedly installed rabbling mechanism, one end is fixedly installed thermocouple port on the right side of the rabbling mechanism, and the thermocouple port upper one end is fixedly installed gauge port.The utility model generates the pH value during measure callus growth by setting wire, ball electrode bubble, glass shell, fluid sealant and liquid filling hole, to ensure beneficial effect that vegetable material grows in suitable pH value.By setting left flabellum, de-burring machine, right wing leaf and mixer, the nutrient and vegetable material for playing and being evenly distributed in fermentation tank in fermentation tank are generated, flat vanes do not injure vegetable material and stirred and beneficial effect that mixing area is larger.

Description

A kind of fermentation tank for cultivating vegetable material callus
Technical field
The fermentation tank field of culture vegetable material callus is the utility model is related to, is specially a kind of culture vegetable material The fermentation tank of callus.
Background technology
At present, people come frequently by the mode of tissue cultures by squamous subculture again and again or nutrient growth Obtain substantial amounts of callus;But such a method not only grow by cycles consumed, and needs more human and material resources;Testing It is very easy to different degrees of pollution occur in journey, prevents people from efficiently, quickly obtaining to obtain substantial amounts of callus, it is impossible to Realize the vegetable material needed for extensive, production in enormous quantities.
Utility model content
The purpose of this utility model is to provide a kind of fermentation tank for cultivating vegetable material callus, for culture now The maturation of fiery crane callus technology, the problem of overcoming above-mentioned processing test material, comprising manual and automatic control mode, automatically Control parameter includes temperature, rotating speed, humidity, air mass flow and pressure.
To achieve the above object, the utility model provides following technical scheme:A kind of culture vegetable material callus Fermentation tank, it is characterised in that:The fermentation tank of the culture vegetable material callus, including fermentation tank, PH electrodes, liquid insulating Layer, bearing, cooling water inlet, rabbling mechanism, thermocouple port, gauge port, air inlet, visor, motor, motor, transmission parts, Mechanical seal, enter hole, sample tap, coolant outlet and thermometer mouth, one end is fixedly installed PH electricity in the middle part of the fermentation tank Pole, the PH electrodes lower end thereof are fixedly installed liquid insulating layer, and the liquid insulating layer lower end thereof is fixedly installed branch Seat, described bearing right upper portion one end are fixedly installed cooling water inlet, and the cooling water inlet left side central portion is fixedly installed Rabbling mechanism, rabbling mechanism right side one end are fixedly installed thermocouple port, the thermocouple port upper one end fixed setting There is gauge port, the gauge port upper one end is fixedly installed air inlet, and air inlet top side is fixedly installed visor, Described visor upper left-hand one end is fixedly installed motor, and described motor left side central portion one end is fixedly installed motor, institute To state lower end thereof in the middle part of motor and be fixedly installed transmission parts, transmission parts bottom middle-end is fixedly installed mechanical seal, One end is fixedly installed into hole on the left of the mechanical seal, it is described enter hole on the left of one end be fixedly connected with sample tap, the sampling Mouth lower end thereof is fixedly installed coolant outlet, and middle side part one end is fixedly installed thermometer mouth under the coolant outlet.
Preferably, the PH electrodes, including wire, ball electrode bubble, glass shell, fluid sealant and liquid filling hole, the wire It is fixedly installed ball electrode bubble on the downside of middle part, the ball electrode bubble bottom side is fixedly installed glass shell, outside the glass Side is fixedly installed fluid sealant in shell inside, and the fluid sealant left upper portion is fixedly installed liquid filling hole.
Preferably, the rabbling mechanism, including left flabellum, de-burring machine, right wing leaf and mixer, on the right side of the left flabellum on Portion one end is fixedly installed de-burring machine, and the de-burring machine lower end thereof is fixedly installed right wing leaf, the right wing leaf left side central portion It is fixedly installed mixer.
Preferably, the fluid sealant is provided with two, and material is gelled using waterproof, the glass shell, and material is using poly- Carbonate resin, ball electrode bubble, formed using the lithium glass fusing blowing with hydrogen function, its resistance value be less than 250 megaohms, 25 degrees Celsius of temperature setting.
Preferably, the left flabellum and right wing leaf, are mutually permanently connected with mixer, the left flabellum and right wing leaf, respectively Three are provided with, using 45 degree of radian swash plates.
Preferably, the mechanical seal, using high-strength gravity type sealed plate.
Compared with prior art, the utility model has the advantages that as follows:
1. the utility model generates measure by setting wire, ball electrode bubble, glass shell, fluid sealant and liquid filling hole PH value during callus growth, to ensure beneficial effect that vegetable material grows in suitable pH value.
2. the utility model is generated and played in fermentation tank by setting left flabellum, de-burring machine, right wing leaf and mixer Evenly distribute the nutrient and vegetable material in fermentation tank, flat vanes do not injure vegetable material and stirred and mixing area Larger beneficial effect.
3. for the utility model by setting mechanical seal, thermocouple port and thermometer mouth, fermentation tank can be adjusted by generating Interior temperature, ensure plant material grown in optimum temperature environment.Suspend or paste suitable for high yield secreting type product Parietal cell cultivates and perfusion culture beneficial effect.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model.
Fig. 2 is this practical PH electrode structures schematic diagram.
Fig. 3 is this practical rabbling mechanism structural representation.
Figure:1 fermentation tank, 2PH electrodes, 21 wires, 22 ball electrodes bubble, 23 glass shells, 24 fluid sealants, 25 liquid filling holes, 3 Liquid insulating layer, 4 bearings, 5 cooling water inlets, 6 rabbling mechanisms, 61 left flabellums, 62 de-burring machines, 63 right wing leaves, 64 mixers, 7 Thermocouple port, 8 gauge ports, 9 air inlets, 10 visors, 11 motor, 12 motors, 13 transmission parts, 14 mechanical seals, 15 enter hole, 16 sample taps, 17 coolant outlets, 18 thermometer mouths
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made The every other embodiment obtained, belong to the scope of the utility model protection.
Fig. 1-3 are referred to, a kind of fermentation tank for cultivating vegetable material callus, to achieve the above object, this practicality is new Type provides following technical scheme:A kind of fermentation tank for cultivating vegetable material callus, it is characterised in that:The culture vegetable material The fermentation tank of callus, including, the middle part one end of fermentation tank 1 is fixedly installed PH electrodes 2, and the downside fixation of the middle part of wire 21 is set Ball electrode bubble 22 is equipped with, ball electrode steeps 22 bottom sides and is fixedly installed glass shell 23, and side is fixed in the inside of glass shell 23 Fluid sealant 24 is provided with, the left upper portion of fluid sealant 24 is fixedly installed liquid filling hole 25.Fluid sealant 24 is provided with two, and material uses Waterproof is gelled, glass shell 23, and material uses polycarbonate resin, ball electrode bubble 22, is fused using the lithium glass with hydrogen function Blowing forms, and its resistance value is less than 250 megaohms, 25 degrees Celsius of temperature setting.By setting wire 21, ball electrode bubble 22, glass Shell 23, fluid sealant 24 and liquid filling hole 25, the pH value during measure callus growth is generated, to ensure that vegetable material exists The beneficial effect grown in suitable pH value.The lower end thereof of PH electrodes 2 is fixedly installed liquid insulating layer 3, liquid insulating 3 lower end thereof of layer are fixedly installed bearing 4, and the right upper portion one end of bearing 4 is fixedly installed cooling water inlet 5, cooling water inlet 5 Left side central portion is fixedly installed rabbling mechanism 6, and left right upper portion one end of flabellum 61 is fixedly installed de-burring machine 62, under de-burring machine 62 Portion one end is fixedly installed right wing leaf 63, and the left side central portion of right wing leaf 63 is fixedly installed mixer 64.Left flabellum 61 and right wing leaf 63, it is mutually permanently connected with mixer 64, left flabellum 61 and right wing leaf 63, three is each provided with, using 45 degree of radian swash plates.It is logical Cross and left flabellum 61, de-burring machine 62, right wing leaf 63 and mixer 64 are set, generate to play in fermentation tank and evenly distribute fermentation tank In nutrient and vegetable material, flat vanes do not injure vegetable material and stir and beneficial effect that mixing area is larger Fruit, the right side one end of rabbling mechanism 6 are fixedly installed thermocouple port 7, and the upper one end of thermocouple port 7 is fixedly installed gauge port 8, instrument The upper one end of table mouth 8 is fixedly installed air inlet 9, and the top side of air inlet 9 is fixedly installed visor 10, the upper left-hand of visor 10 One end is fixedly installed motor 11, and the left side central portion one end of motor 11 is fixedly installed motor 12, the middle part bottom one of motor 12 End is fixedly installed transmission parts 13, and the bottom middle-end of transmission parts 13 is fixedly installed mechanical seal 14, mechanical seal 14, used High-strength gravity type sealed plate.The left side one end of mechanical seal 14 is fixedly installed into hole 15, is entered the left side one end of hole 15 and is fixedly connected with Sample tap 16, the lower end thereof of sample tap 16 are fixedly installed coolant outlet 17, and 17 times middle side part one end of coolant outlet are fixed It is provided with thermometer mouth 18.By setting mechanical seal 14, thermocouple port 7 and thermometer mouth 18, fermentation can be adjusted by generating Temperature in tank, ensure plant material grown in optimum temperature environment.Suitable for high yield secreting type product suspend or Attached cell cultivates and perfusion culture beneficial effect.
In summary:The fermentation tank of the culture vegetable material callus, by setting wire 21, ball electrode bubble 22, glass Glass shell 23, fluid sealant 24 and liquid filling hole 25, the pH value during measure callus growth is generated, to ensure vegetable material The beneficial effect grown in suitable pH value.By setting left flabellum 61, de-burring machine 62, right wing leaf 63 and mixer 64, the nutrient and vegetable material for playing and being evenly distributed in fermentation tank in fermentation tank are generated, flat vanes do not injure plant material Expect and stir and beneficial effect that mixing area is larger, by setting mechanical seal 14, thermocouple port 7 and thermometer mouth 18, the temperature that can be adjusted in fermentation tank is generated, ensures plant material grown in optimum temperature environment.Suitable for height Yield secretion type product suspends or attached cell culture and perfusion culture beneficial effect.
While there has been shown and described that embodiment of the present utility model, for the ordinary skill in the art, It is appreciated that these embodiments can be carried out with a variety of changes in the case where not departing from principle of the present utility model and spirit, repaiied Change, replace and modification, the scope of the utility model are defined by the appended claims and the equivalents thereof.

Claims (6)

  1. A kind of 1. fermentation tank for cultivating vegetable material callus, it is characterised in that:The hair of the culture vegetable material callus Fermentation tank, including fermentation tank (1), PH electrodes (2), liquid insulating layer (3), bearing (4), cooling water inlet (5), rabbling mechanism (6), thermocouple port (7), gauge port (8), air inlet (9), visor (10), motor (11), motor (12), transmission parts (13), mechanical seal (14), enter hole (15), sample tap (16), coolant outlet (17) and thermometer mouth (18), the fermentation tank One end is fixedly installed PH electrodes (2) in the middle part of body (1), and PH electrodes (2) lower end thereof is fixedly installed liquid insulating layer (3), liquid insulating layer (3) lower end thereof is fixedly installed bearing (4), and described bearing (4) right upper portion one end is fixed and set Cooling water inlet (5) is equipped with, cooling water inlet (5) left side central portion is fixedly installed rabbling mechanism (6), the rabbling mechanism (6) right side one end is fixedly installed thermocouple port (7), and thermocouple port (7) upper one end is fixedly installed gauge port (8), Gauge port (8) upper one end is fixedly installed air inlet (9), and air inlet (9) the top side is fixedly installed visor (10), described visor (10) upper left-hand one end is fixedly installed motor (11), described motor (11) left side central portion one end Motor (12) is fixedly installed, lower end thereof is fixedly installed transmission parts (13), the driving section in the middle part of the motor (12) Part (13) bottom middle-end is fixedly installed mechanical seal (14), and one end is fixedly installed into hole on the left of the mechanical seal (14) (15), it is described enter hole (15) on the left of one end be fixedly connected with sample tap (16), sample tap (16) the lower end thereof fixed setting There is a coolant outlet (17), middle side part one end is fixedly installed thermometer mouth (18) under the coolant outlet (17).
  2. A kind of 2. fermentation tank for cultivating vegetable material callus according to claim 1, it is characterised in that:The PH electricity Pole (2) includes wire (21), ball electrode bubble (22), glass shell (23), fluid sealant (24) and liquid filling hole (25), the wire (21) middle part downside is fixedly installed ball electrode bubble (22), and described ball electrode bubble (22) bottom side is fixedly installed glass shell (23), side is fixedly installed fluid sealant (24) in glass shell (23) inside, and fluid sealant (24) left upper portion is fixed It is provided with liquid filling hole (25).
  3. A kind of 3. fermentation tank for cultivating vegetable material callus according to claim 1, it is characterised in that:The stirring Mechanism (6) includes left flabellum (61), de-burring machine (62), right wing leaf (63) and mixer (64), on left flabellum (61) right side Portion one end is fixedly installed de-burring machine (62), and de-burring machine (62) lower end thereof is fixedly installed right wing leaf (63), the right side Flabellum (63) left side central portion is fixedly installed mixer (64).
  4. A kind of 4. fermentation tank for cultivating vegetable material callus according to claim 2, it is characterised in that:The sealing Glue (24) is provided with two materials and is gelled using waterproof, and glass shell (23) material uses polycarbonate resin, the electrode Ball bubble (22) using with hydrogen function lithium glass fusing blowing form, its resistance value be less than 250 megaohms, temperature setting 25 it is Celsius Degree.
  5. A kind of 5. fermentation tank for cultivating vegetable material callus according to claim 3, it is characterised in that:The left fan Leaf (61) and right wing leaf (63), are mutually permanently connected with mixer (64), the left flabellum (61) and right wing leaf (63), each to set There are three, using 45 degree of radian swash plates.
  6. A kind of 6. fermentation tank for cultivating vegetable material callus according to claim 1, it is characterised in that:The machinery Seal (14), using high-strength gravity type sealed plate.
CN201720662405.0U 2017-06-08 2017-06-08 A kind of fermentation tank for cultivating vegetable material callus Expired - Fee Related CN206858582U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201720662405.0U CN206858582U (en) 2017-06-08 2017-06-08 A kind of fermentation tank for cultivating vegetable material callus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201720662405.0U CN206858582U (en) 2017-06-08 2017-06-08 A kind of fermentation tank for cultivating vegetable material callus

Publications (1)

Publication Number Publication Date
CN206858582U true CN206858582U (en) 2018-01-09

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107988074A (en) * 2018-01-25 2018-05-04 吉林冠界生物技术有限公司 The full suspension cell culture reactor of 6000L serum-frees

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107988074A (en) * 2018-01-25 2018-05-04 吉林冠界生物技术有限公司 The full suspension cell culture reactor of 6000L serum-frees
CN107988074B (en) * 2018-01-25 2024-02-06 吉林冠界生物技术有限公司 6000L serum-free full-suspension cell culture reactor

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CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20180109

Termination date: 20180608

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