CN206611945U - A kind of tissue culture bottle for cultivating brassica plant hypocotyl - Google Patents
A kind of tissue culture bottle for cultivating brassica plant hypocotyl Download PDFInfo
- Publication number
- CN206611945U CN206611945U CN201720364020.6U CN201720364020U CN206611945U CN 206611945 U CN206611945 U CN 206611945U CN 201720364020 U CN201720364020 U CN 201720364020U CN 206611945 U CN206611945 U CN 206611945U
- Authority
- CN
- China
- Prior art keywords
- bottle body
- bottle
- tissue culture
- brassica plant
- hypocotyl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Abstract
The utility model provides a kind of tissue culture bottle for cultivating brassica plant hypocotyl, including tissue culture bottle body and bottle cap, and the tissue culture bottle body includes upper bottle body and lower bottle body；The upper bottle body and the lower bottle body nested encryptions, and be rectangular parallelepiped structure, the upper bottle body can be stretched or bounced back along the lower bottle body；It is provided with the outside of the bottleneck of the upper bottle body on the inside of screw thread one, the bottle cap provided with the screw thread two matched with the screw thread one；The body and the bottle cap are made of lighttight black plastic or glass material.Body of the present utility model is double-deck telescopic rectangular parallelepiped structure, and body height can be adjusted flexibly according to the growing state of hypocotyl, hypocotyl is effectively prevent because being influenceed its vertical growth by tissue culture bottle height limitation；Meanwhile, the tissue culture bottle of cuboid can not interspace to each other when putting, and can farthest save space, improve the utilization ratio of tissue culture room.
Description
Technical field
The utility model belongs to a kind of experiment equipment, and in particular to a kind of tissue culture bottle of culture brassica plant hypocotyl.
Background technology
Plant protoplast is a kind of preferable material for studying cell physiological function, is widely used in genetic transformation, thin
The field such as born of the same parents' fusion and cell body mutation.Research for genetic and cell engineering is provided wide by the research of protoplast
Prospect.The hypocotyl of light culture, because it is very low without chlorophyll or content, without green exciting light under fluorescence microscope
Influence, can preferably determine the vigor of protoplast, and have it is demonstrated experimentally that with the isolated protoplast division of hypocotyl
Frequency is high, so that hypocotyl turns into the ideal material of separated protoplast.
In the practical operation of culture hypocotyl, often seed is seeded in common tissue culture bottle, then with shadings such as newspapers
Material is put into tissue culture room after carrying out shading, but general light screening material has poorly sealed and light leak or carried disease germs etc. and asks
Topic, so that hypocotyl can not well grow or easily be contaminated, meanwhile, shading treatment is also brought much not for operation
Just.Also, in separated protoplast, under light culture the hypocotyl length of plant compared with illumination cultivation when be obviously prolonged, it is and general
Tissue culture bottle it is shorter, limit its vertical growth, meanwhile, general tissue culture bottle be generally cylinder, can not when being put in tissue culture room
It is spaced relationship to avoiding, so as to waste the space of tissue culture room.
Utility model content
The problem of existing for prior art, the utility model provides a kind of tissue culture for cultivating brassica plant hypocotyl
Bottle.The technical solution of the utility model is：
A kind of tissue culture bottle for cultivating brassica plant hypocotyl, including tissue culture bottle body and bottle cap, the tissue culture bottle body
Including upper bottle body and lower bottle body；The upper bottle body and the lower bottle body nested encryptions, and be rectangular parallelepiped structure, the upper bottle
Body can be stretched or bounced back along the lower bottle body；Be provided with the outside of the bottleneck of the upper bottle body on the inside of screw thread one, the bottle cap be provided with
The screw thread two that the screw thread one matches；The body and the bottle cap use lighttight black plastic or glass material system
Into.
Further, the upper bottle body is identical with the height of the lower bottle body, is 7~10cm.
Further, the concrete mode of the upper bottle body and the lower bottle body nested encryptions is：The upper bottle body is relative
Two sides center is enclosed provided with raised, and the lower bottle body is provided with chute, institute with the upper bottle body with the two sides center in orientation
State and block is provided with above chute, the raised circle is embedded in the chute.
Further, the bottle cap center is provided with above air-vent, the air-vent and sets black screen membrane.
Further, provided with the rectangle anti-slip veins being interspersed on the outside of the bottle cap.
The beneficial effects of the utility model are：
1) the utility model is made up of light tight material, and hypocotyl can be made preferably to be grown in the environment of complete darkness,
The tedious steps of occlusion process are it also avoid simultaneously and because being polluted using light screening material to plant, improve Success in Experiment
Rate.
2) body of the present utility model is double-deck telescopic rectangular parallelepiped structure, can be flexible according to the growing state of hypocotyl
Body height is adjusted, hypocotyl is effectively prevent because being influenceed its vertical growth by tissue culture bottle height limitation；Meanwhile, cuboid
Tissue culture bottle can not be interspaced to each other when putting, can farthest save space, improve the utilization ratio of tissue culture room.
3) air-vent and black screen membrane also can be set in the bottle cap center, can reduce the pollution of plant and facilitate plant
It is ventilative.
Brief description of the drawings
Fig. 1 is a kind of structural representation of the present utility model；
Wherein 1- upper bottle bodies, 2- lower bottle bodies, 3- projection circles, 4- chutes, 5- screw threads one, 6- screw threads two, 7- air-vents, 8- is black
Color screen membrane, 9- bottle caps, 10- rectangle anti-slip veins, 11- blocks.
Embodiment
The utility model is described in further details with specific embodiment below in conjunction with the accompanying drawings, described is to this practicality
New explanation rather than restriction.
A kind of tissue culture bottle of culture brassica plant hypocotyl as shown in Figure 1, including tissue culture bottle body and bottle cap 9, institute
Stating tissue culture bottle body includes upper bottle body 1 and lower bottle body 2；Upper bottle body 1 and the lower bottle body 2 nested encryptions, concrete mode is：
The relative two sides center of the upper bottle body is provided with raised circle 3, the lower bottle body and two sides of the upper bottle body with orientation
Center is provided with chute 4, and the top of chute 4 is provided with block 11, and the raised circle 3 is embedded in the chute 4, the effect of block
Be prevent upper bottle body be stretched to it is most long after depart from lower bottle body；The upper bottle body 1 and the lower bottle body 2 are rectangular parallelepiped structure, institute
It is highly 7cm to state rectangular parallelepiped structure, and lower bottle body base length and width are 6.5cm, and upper bottle body base length and width are 6cm, upper bottle body bottle
Open height is 1cm, and bottleneck diameter is 6cm, and the upper bottle body 1 can stretch or bounce back along the lower bottle body 2；The upper bottle body 1
Screw thread 1 is provided with the outside of bottleneck, the inner side of bottle cap 9 is provided with the screw thread 26 matched with the screw thread 1；In the bottle cap 9
The heart is provided with air-vent 7, and the top of air-vent 7 sets black screen membrane 8；The outside of bottle cap 9 is additionally provided with the square being interspersed
Shape anti-slip veins 10.The body and the bottle cap are made of lighttight black plastic or glass material.
During using the present apparatus, directly in seed tissue culture bottle can be put into tissue culture room, without being entered again with light screening material
Row shading.
Preferred embodiment of the present utility model has shown and described in described above, but as previously described, it should be understood that it is practical new
Type is not limited to form disclosed herein, is not to be taken as the exclusion to other embodiment, and available for various other groups
Close, change and environment, and above-mentioned teaching or the technology of association area can be passed through in utility model contemplated scope described herein
Or knowledge is modified.And the change and change that those skilled in the art are carried out do not depart from the spirit and scope of utility model, then all
Should be in the protection domain of utility model appended claims.
Claims (5)
1. a kind of tissue culture bottle for cultivating brassica plant hypocotyl, it is characterised in that including tissue culture bottle body and bottle cap, described group
Training bottle body includes upper bottle body and lower bottle body；The upper bottle body and the lower bottle body nested encryptions, and be rectangular parallelepiped structure, institute
Stating upper bottle body can stretch or bounce back along the lower bottle body；It is provided with the outside of the bottleneck of the upper bottle body on the inside of screw thread one, the bottle cap
Provided with the screw thread two matched with the screw thread one；The body and the bottle cap use lighttight black plastic or glass
Material is made.
2. a kind of tissue culture bottle for cultivating brassica plant hypocotyl according to claim 1, it is characterised in that the upper bottle
Body is identical with the height of the lower bottle body, is 7~10cm.
3. a kind of tissue culture bottle for cultivating brassica plant hypocotyl according to claim 2, it is characterised in that the upper bottle
The concrete mode of body and the lower bottle body nested encryptions is：The relative two sides center of the upper bottle body is enclosed provided with raised, institute
Lower bottle body is stated to be provided with above chute, the chute provided with block with the two sides center in orientation with the upper bottle body, it is described convex
Looping is embedded in the chute.
4. a kind of tissue culture bottle for cultivating brassica plant hypocotyl according to claim 3, it is characterised in that the bottle cap
Center is provided with above air-vent, the air-vent and sets black screen membrane.
5. a kind of tissue culture bottle of culture brassica plant hypocotyl according to Claims 1 to 4 any one claim,
It is characterized in that provided with the rectangle anti-slip veins being interspersed on the outside of the bottle cap.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720364020.6U CN206611945U (en) | 2017-04-09 | 2017-04-09 | A kind of tissue culture bottle for cultivating brassica plant hypocotyl |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720364020.6U CN206611945U (en) | 2017-04-09 | 2017-04-09 | A kind of tissue culture bottle for cultivating brassica plant hypocotyl |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206611945U true CN206611945U (en) | 2017-11-07 |
Family
ID=60235874
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720364020.6U Expired - Fee Related CN206611945U (en) | 2017-04-09 | 2017-04-09 | A kind of tissue culture bottle for cultivating brassica plant hypocotyl |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206611945U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109603244A (en) * | 2019-01-08 | 2019-04-12 | 贵州大学 | A kind of clamping device for tissue culture filtering |
-
2017
- 2017-04-09 CN CN201720364020.6U patent/CN206611945U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109603244A (en) * | 2019-01-08 | 2019-04-12 | 贵州大学 | A kind of clamping device for tissue culture filtering |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171107 Termination date: 20180409 |