CN206033739U - Packaging component is reformed transform to aseptic solution reagent bottle lid and bottle lid - Google Patents

Abstract

The utility model provides a packaging component is reformed transform to aseptic solution reagent bottle lid and bottle lid, belongs to the chemical reagent container field for the problem polluted takes place for save and interpolation, collection in -process easily that solve aseptic reagent. The utility model discloses compare with ordinary reagent bottle lid or bottle plug, its characteristics have the pipeline that two air expansion bottles are interior and the bottle is outer, and one of them root canal says that it passes in and out the pipeline to be the sterile solution, and another root canal way is the pressure adjustment pipeline, also have annular groove or screw thread at pipeline both ends, optional, still have the one -way pipe that sealed cap and bent angle are less than 90. The utility model discloses can reduce open surface at the interpolation of aseptic reagent, collection or save in -process and amass, increase the seal, reduce the chance of aseptic reagent and sterile culture thing pollution, especially be suitable for the automation mechanized operation.

Description

A kind of sterile solution reagent bottle cap and bottle cap transformation part combination

Technical field

This utility model belongs to chemical container, more particularly to a kind of sterile solution reagent bottle cap.

Background technology

Biomedicine field is one of domestic and international fastest-rising industry.In the scientific research of biomedicine field, production process The pollution for being related to substantial amounts of microorganism, in vitro cell culture, miscellaneous bacteria or heteroproteose cell be affect experiment or produce success or failure key because Element.With the development of industry, bioreactor has evolved to the cell culture body of kilolitre level, ten thousand upgradings, and antibiotic-free System or fermentation system become the trend of development, to reagent, consumptive material, equipment, the aseptic of Factory Building and technical staff sterile working The requirement of technology also more and more higher.

With Chinese hamster ovary celI（Chinese hamster ovary cell）Culture as a example by, need in incubation add basal medium and Repeatedly add supplementing culture medium, cell culture medium that is commercially available or voluntarily configuring typically is stored in common vial or plastic bottle In, bottle cap is vinyl cover or plug, tampon, and culture medium is added or needs open operation, culture medium to be exposed to ring during supplementing In border, the time is long, provides chance for pollution, has both been likely to result in cell culture system pollution, it is also possible to cause remaining culture medium Pollution.For large-scale bioreactor, as cultivating system is big, need the culture medium of addition many, corresponding open area and The open time is also long, so as to increase the chance of pollution.In addition, after Chinese hamster ovary celI culture terminates or in incubation, collection contains There is the supernatant of target protein, be directed to sterile working.

Therefore, antipollution aseptic reagent container or related tool, accessory, the scientific research, life to biomedicine field are developed Product has great significance.

The content of the invention

It is susceptible to during preservation and addition, collection for the culture medium with sterility requirements or other liquid reagents The problem of pollution, this utility model provide a kind of sterile solution reagent bottle cap and for transforming sterile solution reagent bottle cap zero Part is combined, and technical scheme is as follows：

A kind of sterile solution reagent bottle cap（1）, compared with common reagent bottle cap or bottle stopper, it is characterized in two companies Pipeline in logical bottle and outside bottle（Fig. 1）, wherein one pipeline is sterile solution inlet and outlet piping（2）, another pipeline be pressure adjust Length of tubing（3）.

Reagent bottle bottleneck and bottle cap specification species due to aseptic experiment room or used in sterile workshop is various, for the ease of Above-mentioned technical proposal is realized, carrying out transformation to the bottle cap of existing reagent bottle may be than directly by the life of the method such as compression moulding or injection The two-tube bottle cap for producing different size is more convenient, economical, and it is a kind of common aseptic molten for transforming to be that this this utility model is additionally provided The part combination of liquid reagent bottle cap, by two-tube external member（4）, gasket seal（5）, setting-up piece（6）With 2 nuts（7、8）Etc. group Part is constituted, wherein gasket seal be rubber blanket or other there is the material of sealing effectiveness, other assemblies are rigid, such as stainless Steel or rigid plastics；Wherein two-tube external member is 2 parallelpipeds linked together by rigid structure, and a pipeline is aseptic molten Liquid inlet and outlet piping（2）, another pipeline is pressure regulation tube road（3）, on 2 pipelines, have screw thread.

Preferably, described bottle cap or part combination, have at the two ends in sterile solution inlet and outlet piping and pressure regulation tube road Annular groove or screw thread, for fixation and sealing between pipeline and solution conduit or seal cap.

Preferably, described bottle cap or part combination, also with 2 seal caps（9）, can be by bayonet socket, rotation mouth or friction Power airtight connection is on sterile solution inlet and outlet piping or pressure regulation tube road.

Preferably, described bottle cap or part combination, the also unidirectional conduit with a bent angle less than 90 °（10）, conduit One end can be with described sterile solution inlet and outlet piping（2）By bayonet socket, rotation mouth or frictional force airtight connection, the other end is interior nearby It is equipped with check valve（11）, prevent solution from flowing backwards.

The beneficial effects of the utility model are, can reduce in the addition of aseptic reagent, during collecting or preserving open area, Increase seal, reduce the chance of aseptic reagent and Aseptic culture material pollution, be particularly suited for automation mechanized operation.

Description of the drawings

Fig. 1：A kind of sectional side elevation of sterile solution reagent bottle cap；

Fig. 2：A kind of top view of sterile solution reagent bottle cap；

Fig. 3：A kind of each component diagram for transforming the part combination of sterile solution reagent bottle cap；

Fig. 4：A kind of assembling schematic diagram of the part combination for transforming sterile solution reagent bottle cap and bottle cap（Sectional side elevation）；

Fig. 5：A kind of assembling schematic diagram of the part combination for transforming sterile solution reagent bottle cap and bottle cap（Top view）；

Fig. 6：A kind of assembling schematic diagram of the part combination for transforming sterile solution reagent bottle cap and bottle cap（Upward view）；

Fig. 7：Unidirectional conduit sectional side elevation；

Fig. 8：Add the schematic diagram of sterile solution to bioreactor using the bottle cap that this utility model is manufactured；

Fig. 9：The schematic diagram of sterile solution is collected from bioreactor using the bottle cap that this utility model is manufactured；

Figure 10：Unidirectional effective method schematic diagram；

Figure 11：The usage schematic diagram of seal cap；

Figure 12：Pressure monitoring method schematic diagram.

Specific embodiment

Embodiment 1, a kind of sterile solution reagent bottle cap

A kind of sterile solution reagent bottle cap（1）, as shown in Figure 1 and Figure 2, can be rotated on aseptic reagent bottle by screw socket, With the pipeline in two air expansion bottles and outside bottle on bottle cap, wherein one pipeline is sterile solution inlet and outlet piping（2）, another pipe Road is pressure regulation tube road（3）, respectively have annular groove at the two ends of 2 pipelines.

The bottle cap is polypropylene plastics material, can be manufactured by compression molding：The polypropylene material for mixing is inhaled by Material sucking machine Enter plasticator gun barrel, after being heated to semi-molten plastifying state in gun barrel, in mold cavity, upper/lower die is closed squeezing out quantitative Mould, compression moulding cooling and shaping, the demoulding, then through cutting ring, cushioning, complete the production of compression moulding lid.

Embodiment 2, a kind of part combination for transforming sterile solution reagent bottle cap

A kind of part combination for transforming sterile solution reagent bottle cap, as shown in figure 3, by two-tube external member（4）, sealing gasket Piece（5）, setting-up piece（6）With 2 nuts（7、8）Composition, wherein two-tube external member are put down by 2 that rigid structure links together Row pipeline, a pipeline are sterile solution inlet and outlet piping, and another pipeline is pressure regulation tube road, has screw thread on 2 pipelines, Respectively there is annular groove at the two ends of 2 pipelines；Gasket seal in part combination is the rubber blanket with 2 holes, other assemblies For stainless steel, simple structure can be by conventional rubber blanket and the manufacture of rustless steel processing and forming technology.

The using method of part combination is, in existing general reagent bottle cap（16）The upper method system by machine drilling Two holes that pass through can two-tube external member are made, then by gasket seal（5）, bottle cap（16）, setting-up piece（6）Sequentially pass through two-tube 2 pipelines of external member, be superimposed on connection 2 pipelines rigid structure on, then by 2 nuts be respectively sleeved in 2 pipelines it Go up and tighten, the structure for being formed as Figure 4-Figure 6, becomes a kind of bottle for carrying the pipeline in two air expansion bottles and outside bottle Lid.

The auxiliary that the part combination can also be matched, including 2 seal caps（9）, it is quality of rubber materials, may be stuck in 2 pipelines （Sterile solution inlet and outlet piping, pressure regulation tube road）Bottle external port on, as shown in figure 11, seal；One is carried also Unidirectional conduit of the bent angle less than 90 °（10）, as shown in fig. 7, main body is stainless steel, there is cushion rubber one end of conduit, can be with nothing Bacterium solution inlet and outlet piping（2）Bottle external port airtight connection, as shown in Figure 10, near the other end, be built-in with check valve（11）, should Unidirectional conduit can prevent from solution from blowing back in reagent bottle polluting.

Embodiment 3, using this utility model manufacture bottle cap to bioreactor add sterile solution

By sterile solution inlet and outlet piping（2）With flexible pipe airtight connection, the other end and bioreactor of flexible pipe（14）For The port airtight connection of addition culture medium, using peristaltic pump（13）Sterile solution is driven to flow from reagent bottle to bioreactor； Pressure regulation tube road and the filter that aperture is 0.22 micron（12）Airtight connection, keeps the balance of air pressure in bottle, and prevents air In microorganism enter bottle in.Method of attachment is as shown in Figure 8.

Embodiment 4, using this utility model manufacture bottle cap from bioreactor collect sterile solution

By sterile solution inlet and outlet piping（2）With flexible pipe airtight connection, the other end and bioreactor of flexible pipe（14）For Go out the port airtight connection of liquid, using peristaltic pump（13）Sterile solution is driven to flow from bioreactor to reagent bottle；Pressure is adjusted Length of tubing and the filter that aperture is 0.22 micron（12）Airtight connection, keeps the balance of air pressure in bottle, and prevents micro- in air It is biological to enter in bottle.Method of attachment is as shown in Figure 9.

Embodiment 5, the reagent bottle of the bottle cap for being provided with this utility model manufacture are used for storing sterile solution

When the sterile solution being provided with the reagent bottle of bottle cap of this utility model manufacture needs temporarily to store, by 2 Seal cap（9）It is installed in the bottle external port in sterile solution inlet and outlet piping and pressure regulation tube road, as shown in figure 11, then will examination Agent bottle is stored in warehouse.Some solution needs to monitor pressure in Storage period, at this moment can be by a pressure gauge（15）It is installed on nothing In the bottle external port in bacterium solution inlet and outlet piping or pressure regulation tube road, the port of another pipeline is sealed up with seal cap, such as Figure 12 It is shown, then reagent bottle is stored in reagent rack.

Claims (5)

1. a kind of sterile solution reagent bottle cap, it is characterised in that with two air expansion bottles with bottle outside pipeline, a pipeline is Sterile solution inlet and outlet piping, another pipeline are pressure regulation tube road.

2. a kind of part combination for transforming sterile solution reagent bottle cap, it is characterised in that by two-tube external member, gasket seal, Setting-up piece and 2 nut compositions；Wherein two-tube external member is 2 pipelines for being connected in parallel at together by rigid structure, a pipe Road is sterile solution inlet and outlet piping, and another pipeline is pressure regulation tube road, has screw thread on 2 pipelines.

3. the bottle cap or part combination described in claim 1 or 2, it is characterised in that sterile solution inlet and outlet piping and pressure are adjusted There are annular groove or screw thread in the two ends of pipeline, for fixation and sealing between pipeline and solution conduit or seal cap.

4. the bottle cap or part combination described in claim 1 or 2, it is characterised in that also with 2 seal caps, can by bayonet socket, Rotation mouth or frictional force airtight connection are on sterile solution inlet and outlet piping or pressure regulation tube road.

5. the bottle cap or part combination described in claim 1 or 2, it is characterised in that the leading less than 90 ° also with a bent angle Pipe, one end of conduit can be built-in with check valve, prevent molten with described sterile solution inlet and outlet piping airtight connection near the other end Liquid flows backwards.