CN205839034U - Tissue Culture Flask - Google Patents
Tissue Culture Flask Download PDFInfo
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- CN205839034U CN205839034U CN201620809809.3U CN201620809809U CN205839034U CN 205839034 U CN205839034 U CN 205839034U CN 201620809809 U CN201620809809 U CN 201620809809U CN 205839034 U CN205839034 U CN 205839034U
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- lid
- air
- vent
- tissue culture
- culture flask
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Abstract
The utility model discloses a kind of Tissue Culture Flask, including bottle, bottleneck and bottle cap, bottle cap includes: the first lid, and the upper end of the first lid offers some first air-vents；Second lid, plane along the first place, lid upper surface is rotationally connected with the first lid, between first lid and the second lid, the rotary shaft by being arranged at the first lid centre position connects, the upper surface of the second lid is provided with the second air-vent, when the second lid rotates around rotary shaft, the second air-vent and the first air-vent cooperatively form different size of air-vent；The hydrophobic filter membrane covering the first air-vent it is provided with on the diapire of the first lid portion inside.Realize the regulation to air-vent by rotating the second lid, improve the breathability of bottle cap；First lid, in renovating setting, effectively reduces the probability being infected with germs when bottle cap is positioned on operating board, it is provided that cell cultivates a more aseptic environment.
Description
Technical field
This utility model relates to technical field of stem cell culture, more specifically, it relates to a kind of Tissue Culture Flask.
Background technology
Cultivation to cell needs to carry out under certain conditions, such as temperature, pH value, osmotic pressure, nutrient, water, light, nothing
Bacterium condition, gas, and the requirement to gas, zooblast mainly needs be continuing to supply oxygen and get rid of carbon dioxide, thus
Tissue Culture Flask is to the isolation of antibacterial and oxygen or the supply of carbon dioxide, and the eliminating of carbon dioxide or oxygen is extremely important.
In existing Tissue Culture Flask, mainly including body, bottleneck and bottle cap, do not have ventilating structure, it is mainly by controlling bottle
The elasticity of lid controls the circulation of oxygen and carbon dioxide, and the flowing of these gases is all gaps connected with bottleneck from bottle cap
Middle entrance, such mode can supply the gas of culture bottle and flow, but poor air permeability, also make antibacterial enter bottle simultaneously
In, destroy the living environment of cell；And it is difficult to get hold of the elasticity of bottle cap, easily damages culture bottle.
The Chinese patent that public security number is CN201933092U discloses a kind of Tissue Culture Flask, including body and bottle cap, bottle
Being provided with bottleneck with it, bottleneck upper end is bottleneck, and described bottle cap offers 1 or the aperture of more than 1, and in bottle cap inside cover
Face is close to a metafiltration film, and above-mentioned aperture is completely covered by this filter membrane, and the aperture that Tissue Culture Flask is offered increases ventilative
Property, make the gaseous exchange inside and outside bottle unobstructed, meet the demand to gas during cell is cultivated, and the inside cover at the position at aperture
Face is close to, with a metafiltration film, both to completely cut off the entrance of antibacterial, the most not hinder gas exchange, it is ensured that the cell in culture bottle
Culture environment.But it is different that different cells cultivate the requirement to breathability, and the Tissue Culture Flask of different size size due to
The cell number cultivated is different, so gas permeability requirements is the most different, above-mentioned bottle cap universal performance is low.
Utility model content
The deficiency existed for prior art, the purpose of this utility model is to provide a kind of Tissue Culture Flask, it is possible to change
Become the breathability of bottleneck, it is adaptable to different size size and the different types of cell of cultivation.
For achieving the above object, this utility model provides following technical scheme:
A kind of Tissue Culture Flask, including bottle, bottleneck and bottle cap, described bottle cap includes:
First lid, the upper end of described first lid offers some first air-vents；
Second lid, the plane along the first place, lid upper surface is rotationally connected with described first lid, and described first
Between lid and the second lid, the rotary shaft by being arranged at the first lid centre position connects, the upper surface of described second lid
Being provided with the second air-vent, when described second lid rotates around rotary shaft, described second air-vent and the first air-vent are joined
Close and form different size of air-vent；
The hydrophobic filter membrane covering described first air-vent it is provided with on the diapire of described first lid portion inside.
By using technique scheme, rotate the second lid and the second air-vent and the first air-vent are cooperatively formed not
With the air-vent of size, cultivate or used by the Tissue Culture Flask of different size for different cells so that this bottle cap according to
Versatility；And on the diapire near the first lid portion inside of bottleneck, it is provided with the hydrophobic filter membrane covering the first air-vent, energy
Aseptic gas exchange is enough provided, reduces the risk polluted so that cell is cultivated and more standardized.
Further, the side of described second lid is provided with anti-slip part.
By using technique scheme, owing to needing to rotate the second lid when regulating air-vent size, thus anti-skidding
The setting in portion can increase rotation friction so that convenient operate the second lid of staff.
Further, the limit for limiting the second lid rotational angle it is provided with between described first lid and the second lid
Position mechanism.
By using technique scheme, arranging of position-limit mechanism one is to facilitate staff's rotational angle, it is possible to fast
Speed finds the size of required air-vent, and two are prevented from the second lid in the case of setting air-vent size occurs to turn
Dynamic, thus cause air-vent generation deviation, affect cell and preferably cultivate.
Further, the described second U-shaped setting of lid, and suitable with the shape of the first lid, described position-limit mechanism
Including the card-bit part being arranged on the second lid inwall, and be arranged on the first lid outer wall suitable with described card-bit part
Draw-in groove.
By using technique scheme, after the second lid turns an angle, card-bit part with draw-in groove clamping, thus can be incited somebody to action
Second lid limits, and prevents it to be rotated further.
Further, described card-bit part is provided with one, and described draw-in groove is provided with several.
By using technique scheme, draw-in groove is provided with several, is equivalent to there are several shelves when regulating air-vent
Position, simpler convenience during regulation.
Further, described first lid includes the connecting portion being connected with bottleneck, and sets in renovating with described connecting portion
The turning cover part put, described connecting portion and turning cover part are by snap-fitted.
By using technique scheme, owing to needing an aseptic environment when cultivating cell, in order to prevent bottle cap
Part antibacterial it is infected with, so by the first lid in renovating setting, so, effectively reducing by bottle when being placed on operating board
The probability that lid is infected with germs so that whole culture environment is more partial to asepticization.
Further, described connecting portion and turning cover part junction are provided with sealing ring.
By use technique scheme, sealing ring arrange one to be prevented from gas from junction pass in and out, it is right to affect
The supply of Tissue Culture Flask and aerofluxus.
Further, the upper side of described connecting portion is provided with a connecting piece, and described a connecting piece is away from connection
The one end in portion is provided with rotating shaft, and the lateral surface of described turning cover part is provided with the second connection sheet, and described second connection sheet offers
The jack being connected with described axis of rotation, and be provided with in described rotating shaft for the fixing retaining mechanism renovating angle.
By using technique scheme, the rotating shaft on a connecting piece is rotationally connected with the second jack being connected on sheet
Achieve the flip action of turning cover part, and in rotating shaft, be provided with retaining mechanism, it is possible to the turning cover part of rotation is fixed on necessarily
Position so that it is will not hinder one's work personnel's operation to Tissue Culture Flask.
Further, described retaining mechanism includes the storage tank being opened on the second connection sheet, the notch of described storage tank
Connection jack, is provided with fixture block, is provided with elastic component, described bullet between described fixture block and the bottom land of storage tank in described storage tank
Described fixture block is pushed into outside the notch of storage tank by property part in its natural state, described rotating shaft offers and described fixture block clamping
Groove.
By using technique scheme, when rotating turning cover part, fixture block and other location contacts of rotating shaft, at elastic component
Effect under fixture block shrink in storage tank, until turning cover part turns to certain angle, fixture block sticks in groove, the reset of elastic component
In fixture block is advanced groove by power, it is achieved thereby that the clamping of turning cover part.
Further, described elastic component includes elastic blob of viscose or spring.
Compared with prior art, the utility model has the advantages that:
1, by rotating the second lid realization regulation to air-vent, the breathability of bottle cap is improved；
2, the first lid is in renovating setting, effectively reduces the probability being infected with germs when bottle cap is positioned on operating board, it is provided that
Cell cultivates a more aseptic environment.
Accompanying drawing explanation
Fig. 1 is overall structure schematic diagram of the present utility model；
Fig. 2 is the exploded perspective view of bottle cap；
Fig. 3 is the enlarged drawing in A portion in Fig. 2.
Reference: 1, bottle；2, bottleneck；3, bottle cap；4, the first lid；41, the first air-vent；42, rotary shaft；5,
Two lids；6, the second air-vent；7, hydrophobic filter membrane；8, anti-slip part；9, card-bit part；10, draw-in groove；11, connecting portion；12, turning cover part；
13, hasp；14, a connecting piece；15, rotating shaft；16, second connects sheet；17, jack；18, storage tank；19, fixture block；20, elastic
Part；21, groove.
Detailed description of the invention
Below in conjunction with the accompanying drawings and embodiment, this utility model is described in detail.
A kind of Tissue Culture Flask, with reference to Fig. 1, the bottleneck 2 in angle shape being connected with bottle 1 including bottle 1 and bottle
Lid 3.
With reference to Fig. 2, bottle cap 3 includes the first lid 4 and the second lid 5, and the upper end of the first lid 4 offers U-shaped recessed
The portion of falling into, and end face has offered some first air-vents 41 thereon；The upper surface of this first lid 4 is provided with N number of region, and N is
Even number, the first air-vent 41 is arranged on alternate region.Second lid 5 is rotationally connected with the first lid 4 by rotary shaft 42,
The shape of the second lid 5 matches with U-shaped depressed part, and can be along the plane and first at the first place, lid 4 upper surface
Lid 4 is rotationally connected, and the upper surface of the second lid 5 is provided with the second air-vent 6, and the upper surface of the second lid 5 be arranged to
The shape that first lid 4 is identical, when the second lid 5 rotates around rotary shaft 42, the second air-vent 6 is joined with the first air-vent 41
Close and form different size of air-vent, to adapt to the cultivation of different types of cell or the Tissue Culture Flask of different size size,
Make bottle cap 3 according to versatility.
It is provided with a card-bit part 9 on second lid 5 inwall, the first lid 4 outer wall is provided with several and is uniformly arranged
The draw-in groove 10 suitable with card-bit part 9, such setting can limit the rotational angle of the second lid 5 to adjust the big of air-vent
Little.It addition, the outside of the second lid 5 is provided with anti-slip part 8, this anti-slip part 8 is made up of some the fins being vertically arranged.First
It is provided with the hydrophobic filter membrane 7 covering the first air-vent 41 on diapire within lid 4, exchanges for aseptic gas, it is provided that give thin
One gnotobasis of born of the same parents' culture bottle.
First lid 4 includes the connecting portion 11 threadeded with bottleneck 2, and with connecting portion 11 in renovating renovating of setting
Portion 12, in conjunction with Fig. 3, the upper side of connecting portion 11 is provided with a connecting piece 14, and at a connecting piece 14 away from connecting portion
One end of 11 is provided with rotating shaft 15, and the lateral surface of turning cover part 12 is provided with the second connection sheet 16, and the second connection sheet 16 offers
The jack 17 being rotationally connected with rotating shaft 15, when rotating shaft 15 and jack 17 grafting, turning cover part 12 is in transmission connection with connecting portion 11.Separately
Outward, connect second and offered storage tank 18 on sheet 16, the notch connection jack 17 of storage tank 18, and arrange in storage tank 18
Fixture block 19 spherical in shape, is provided with elastic component 20 between this fixture block 19 and bottom land of storage tank 18, and this elastic component 20 is at nature
Under state, fixture block 19 is pushed into outside the notch of storage tank 18, and symmetrical in rotating shaft 15 has offered two recessed with fixture block 19 clamping
Groove 21, when fixture block 19 and groove 21 snap-fit engagement, turning cover part 12 and connecting portion 11 cover or open in 180 degree with connecting portion 11
Open, it is therefore prevented that turning cover part 12 is rocked the personnel of hindering one's work and added culture medium or culture medium is poured out cell in Tissue Culture Flask
Culture bottle.Wherein, elastic component 20 can be elastic gum block or spring, uses spring in the present embodiment.
First lid 4 side relative with a connecting piece 14 is provided with hasp 13, for by turning cover part 12 and connecting portion
11 fasten, and are provided with sealing ring, in order to be supplied to Tissue Culture Flask one more in connecting portion 11 and turning cover part 12 junction
Aseptic environment.
The operation principle of this Tissue Culture Flask:
In Tissue Culture Flask during frame culture medium, open hasp 13, rotate turning cover part 12, until fixture block 19 sticks into groove 21
Interior turning cover part 12 is fixed, until after having added culture medium, more just turning cover part 12 fastens.Then according to kind and the bottle of cell
The size of body 1, rotates the second lid 5 to regulate the size of air-vent.
The above is only preferred implementation of the present utility model, and protection domain of the present utility model is not limited merely to
Above-described embodiment, all technical schemes belonged under this utility model thinking belong to protection domain of the present utility model.Should refer to
Go out, for those skilled in the art, without departing from the some improvement under this utility model principle premise and
Retouching, these improvements and modifications also should be regarded as protection domain of the present utility model.
Claims (10)
1. a Tissue Culture Flask, including bottle (1), bottleneck (2) and bottle cap (3), it is characterised in that described bottle cap (3) including:
First lid (4), the upper end of described first lid (4) offers some first air-vents (41)；
Second lid (5), the plane along the first lid (4) place, upper surface is rotationally connected with described first lid (4), described
Between first lid (4) with the second lid (5), the rotary shaft (42) by being arranged at the first lid (4) centre position is connected, institute
The upper surface stating the second lid (5) is provided with the second air-vent (6), when described second lid (5) rotates around rotary shaft (42)
Time, described second air-vent (6) and the first air-vent (41) cooperatively form different size of air-vent；
It is provided with, on the diapire that described first lid (4) is internal, the hydrophobic filter membrane (7) covering described first air-vent (41).
Tissue Culture Flask the most according to claim 1, it is characterised in that the side of described second lid (5) is provided with anti-
Cunning portion (8).
Tissue Culture Flask the most according to claim 2, it is characterised in that described first lid (4) and the second lid (5) it
Between be provided with the position-limit mechanism for limiting the second lid (5) rotational angle.
Tissue Culture Flask the most according to claim 3, it is characterised in that the U-shaped setting of described second lid (5), and with
The shape of the first lid (4) is suitable, and described position-limit mechanism includes the card-bit part (9) being arranged on the second lid (5) inwall, with
And the draw-in groove (10) suitable with described card-bit part (9) being arranged on the first lid (4) outer wall.
Tissue Culture Flask the most according to claim 4, it is characterised in that described card-bit part (9) is provided with one, described card
Groove (10) is provided with several.
Tissue Culture Flask the most according to claim 1, it is characterised in that described first lid (4) includes with bottleneck (2) even
The connecting portion (11) connect, and with described connecting portion (11) in renovating the turning cover part (12) of setting, described connecting portion (11) with turn over
Cap (12) is coordinated by hasp (13).
Tissue Culture Flask the most according to claim 6, it is characterised in that described connecting portion (11) is connected with turning cover part (12)
Place is provided with sealing ring.
Tissue Culture Flask the most according to claim 7, it is characterised in that the upper side of described connecting portion (11) is provided with
A connecting piece (14), described a connecting piece (14) is provided with rotating shaft (15) away from one end of connecting portion (11), described in renovate
The lateral surface in portion (12) is provided with the second connection sheet (16), described second connection sheet (16) offers and described rotating shaft (15) turns
The jack (17) being dynamically connected, and be provided with in described rotating shaft (15) for the fixing retaining mechanism renovating angle.
Tissue Culture Flask the most according to claim 8, it is characterised in that described retaining mechanism includes being opened in the second connection
Storage tank (18) on sheet (16), notch connection jack (17) of described storage tank (18), described storage tank is provided with in (18)
Fixture block (19), is provided with elastic component (20) between the bottom land of described fixture block (19) and storage tank (18), described elastic component (20) exists
Under naturalness, described fixture block (19) is pushed into outside the notch of storage tank (18), described rotating shaft (15) offers and described card
The groove (21) of block (19) clamping.
Tissue Culture Flask the most according to claim 9, it is characterised in that described elastic component (20) include elastic blob of viscose or
Spring.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620809809.3U CN205839034U (en) | 2016-07-27 | 2016-07-27 | Tissue Culture Flask |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620809809.3U CN205839034U (en) | 2016-07-27 | 2016-07-27 | Tissue Culture Flask |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205839034U true CN205839034U (en) | 2016-12-28 |
Family
ID=57641626
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620809809.3U Active CN205839034U (en) | 2016-07-27 | 2016-07-27 | Tissue Culture Flask |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205839034U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023151260A1 (en) * | 2022-02-11 | 2023-08-17 | 辛普森国际生命科技(天津)有限公司 | Cell culture flask |
-
2016
- 2016-07-27 CN CN201620809809.3U patent/CN205839034U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023151260A1 (en) * | 2022-02-11 | 2023-08-17 | 辛普森国际生命科技(天津)有限公司 | Cell culture flask |
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| GR01 | Patent grant |