CN205786658U - The immunochromatography detecting system of dual frequency reception principle is excited based on single-frequency - Google Patents
The immunochromatography detecting system of dual frequency reception principle is excited based on single-frequency Download PDFInfo
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- CN205786658U CN205786658U CN201620702769.2U CN201620702769U CN205786658U CN 205786658 U CN205786658 U CN 205786658U CN 201620702769 U CN201620702769 U CN 201620702769U CN 205786658 U CN205786658 U CN 205786658U
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Abstract
The immunochromatography detecting system of dual frequency reception principle is excited based on single-frequency.Provide a kind of immunochromatography detecting system exciting dual frequency reception principle based on single-frequency.Including ultraviolet/visible spectrophotometer, information acquisition unit and the information process analysis unit being attached thereto, described ultraviolet/visible spectrophotometer is for determining the maximum excitation wavelength of target detection thing；Described information acquisition unit is for carrying out optical scanning to the target detection thing on test strip surface；Described information process analysis unit for carrying out Treatment Analysis to the described signal of telecommunication, thus output detections result.This utility model is in use, the fluorescence that target detection thing in test strip sends is divided into the light of two frequency ranges through two points of mirrors, again the light intensity of the light of two frequency ranges is normalized, thus eliminating the detection substrate interference to two wave bands, the light intensity of the fluorescence then sent by described target detection thing obtains the concentration information of target detection thing.
Description
Technical field
This utility model belongs to quarantine detection technique field, relates to a kind of immunochromatography detecting system and method, specifically relates to
And immunochromatography detecting system and the detection method thereof of dual frequency reception principle is excited based on single-frequency.
Background technology
Fluorescence immune chromatography technology is easy and simple to handle, quick because of it, and has the highest specificity and susceptiveness, necessarily will take
For colloidal gold immunochromatographimethod technology, and it is generalized to Clinical detection and the antibiosis of the diseases such as CRP, HCG, myocardial damage, acquired immune deficiency syndrome (AIDS)
The field of detection of food safety such as element, clenbuterol hydrochloride, tripolycyanamide.
Fluorescent test paper strip not only has using value in clinical medicine application, will have more in civilian medical health maintenance field
Wide market, it has following several big advantage: 1) cheap, test strips production cost is relatively low, detects as routine health
Easily-consumed products, will not be to the biggest financial burden of user increase；2) easy and simple to handle, easily-learned easily mastered, the professional skill to user
Level and schooling are less demanding, are extremely suitable for commercial market；3) detection is quickly, just can take detection knot in the short time
Really, it is not necessary to wait；4) it is suitable for index wide, can be applicable to multiple antigen and antibody carrier, and detection sensitivity is high；5) can be applicable to
Carry out quantifying to monitor continuously and personalized health guarantee.
Due to the fast development of fluorescence immune chromatography technical need, fluoroscopic examination precision and sensitivity are proposed higher
Requirement.At present, maximum during fluorescence immune chromatography detection interference source, it is simply that the fluorescence interference of substrate in detection sample, by
In interference substrate and target detection thing also exist in detection sample, and disturb fluorescence main peak and the target detection thing of substrate
Main peak close, thus detecting instrument cannot distinguish differentiation interference substrate and target detection thing.Present stage grow up each
Plant modulation of source method, background magnetic field and the impact of background not homology spectrum can be eliminated, but some interference substrates are by homology
Excitation source irradiates, and fluorescent modulation frequency is consistent with the frequency of detectable substance, so substrate cannot be eliminated by modulation of source method
Fluorescence disturbs.(such as Chinese patent, " one utilizes time resolution fluorescence spectral to characterize to have developed again time-resolved fluorescence technology in the recent period
The method and apparatus of material magnetic effect "), it is intended to by fluorescence decay time control, distinguish long decaying phosphor cycle or short decay
Fluorescence target cycle detectable substance and substrate, thus eliminate Substrate fluorescence impact.But, in the test event of whole blood, blood red egg
Wait Substrate fluorescence damped cycle to be close with the damped cycle of lanthanide series rare-earth elements in vain, be all hundreds of delicate rank, so the time
Resolved fluorometric technology is also difficult to solve Substrate fluorescence interference, but during the concentration of detection target detection thing, needs to pass through
The intensity of fluorescence obtains the concentration of target detection thing, and the most existing fluorescence immune chromatography technology can accurately not obtain target
The concentration of detectable substance.
Utility model content
The purpose of this utility model is the shortcoming overcoming above-mentioned prior art, it is provided that one excites double frequency based on single-frequency
The immunochromatography detecting system of record principle, this system can effectively eliminate the interference of Substrate fluorescence, and can accurately obtain
Take the concentration information of target detection thing.
The technical solution of the utility model is: includes ultraviolet/visible spectrophotometer, information acquisition unit and is attached thereto
Information process analysis unit,
Described ultraviolet/visible spectrophotometer is for determining the maximum excitation wavelength of target detection thing；
Described information acquisition unit is for carrying out optical scanning to the target detection thing on test strip surface, and it is to target
Detectable substance projects irradiating light beam, to receive the reflected fluorescent light of reagent paper, and described reflected fluorescent light is converted to the signal of telecommunication；
Described information process analysis unit for carrying out Treatment Analysis to the described signal of telecommunication, thus output detections result,
Described information acquisition unit include excitation source (1), semi-transparent semi-reflecting lens (2), lens (3), two points of mirrors (4) and
For detecting first sensor (5) and second sensor (6) of the light intensity of the fluorescence received；
The directional light that excitation source (1) sends is irradiated to detection examination successively after semi-transparent semi-reflecting lens (2) reflection and lens (3)
On the target detection thing on paper slip surface, the fluorescence that target detection thing ejects after semi-transparent semi-reflecting lens (2) transmission again through two points
Mirror (4) reflection and transmission, wherein, the fluorescence that two points of mirrors (4) reflect is irradiated on the photosurface of first sensor (5), and two
The fluorescence that point mirror (4) transmits is irradiated on the photosurface of the second sensor (6), the outfan of first sensor (5) and the
The outfan of two sensors (6) all inputs with described information process analysis unit are connected.
The fluorescence that described two points of mirrors (4) reflect is irradiated to the light of described first sensor (5) through the first optical filter (7)
On quick.
The fluorescence that described two points of mirrors (4) transmit is irradiated to the light of described second sensor (6) through the second optical filter (8)
On quick.
The directional light that described excitation source (1) sends incides described semi-transparent semi-reflecting lens (2) back reflection to lens (3),
Angle between directional light and the directional light of reflection that wherein said semi-transparent semi-reflecting lens (2) is incident is 45～135 °.
Described information process analysis unit uses single-chip microcomputer.
This utility model has the advantages that this utility model in use, the target in test strip
The fluorescence that detectable substance sends is divided into the light of two frequency ranges, first sensor and the second sensor to detect described two respectively through two points of mirrors
The light intensity of the light of individual frequency range, then the light intensity of the light of two frequency ranges is normalized, thus obtain target detection thing and send
The light intensity of fluorescence, thus eliminate the detection substrate interference to two wave bands, then by described target detection thing send glimmering
The light intensity of light obtains the concentration information of target detection thing, thus effectively improves the accuracy and speed of the concentration information detected.
Accompanying drawing explanation
Fig. 1 is principle assumption diagram of the present utility model,
Fig. 2 is workflow of the present utility model,
Fig. 3 is the spectrogram in this utility model embodiment,
Fig. 4 is calibration map in this utility model embodiment,
Fig. 5 is calibration curve and the equation of the first six group data in this utility model embodiment,
Fig. 6 is calibration curve and the equation of rear six groups of data in this utility model embodiment；
Figure 1 is excitation source, 2 is semi-transparent semi-reflecting lens, 3 is lens, 4 is two points of mirrors, 5 is first sensor, 6 is second
Sensor, 7 be the first optical filter, 8 be the second optical filter.
Detailed description of the invention
Below in conjunction with the accompanying drawings this utility model is described in further detail.
As it is shown in figure 1, the immunochromatography detecting system exciting dual frequency reception principle based on single-frequency of the present utility model, including
Ultraviolet/visible spectrophotometer, information acquisition unit and the information process analysis unit being attached thereto,
Described ultraviolet/visible spectrophotometer (utilizes ultraviolet/visible for the maximum excitation wavelength determining target detection thing
Spectrophotometric determination target detection thing, at the absorption curve of 280-760nm, finds the strongest characteristic absorption peak pair of target detection thing
Answer peak position, be the maximum excitation wavelength of sample)；
Described information acquisition unit is for carrying out optical scanning to the target detection thing on test strip surface, and it is to target
Detectable substance projects irradiating light beam, to receive the reflected fluorescent light of reagent paper, and described reflected fluorescent light is converted to the signal of telecommunication；
Described information process analysis unit for carrying out Treatment Analysis to the described signal of telecommunication, thus output detections result；
Described information acquisition unit includes excitation source 1, semi-transparent semi-reflecting lens 2, lens 3, two points of mirrors 4 and receives for detection
The first sensor 5 of the light intensity of fluorescence and the second sensor 6；
The directional light that excitation source 1 sends reflects through semi-transparent semi-reflecting lens 2 successively and is irradiated to test strip table after lens 3
On the target detection thing in face, the fluorescence that target detection thing ejects reflect through two points of mirrors 4 again after semi-transparent semi-reflecting lens 2 transmission and
Transmission, wherein, the fluorescence that two points of mirrors 4 reflect is irradiated on the photosurface of first sensor 5, and two points of mirrors 4 transmit
Fluorescence is irradiated on the photosurface of the second sensor 6, the outfan of first sensor 5 and the outfan of the second sensor 6 all with
The input of described information process analysis unit is connected.
In order to obtain purer fluorescence, the fluorescence that described two points of mirrors 4 reflect is irradiated to described through the first optical filter 7
On the photosurface of first sensor 5；The fluorescence that described two points of mirrors 4 transmit is irradiated to described second through the second optical filter 8 and passes
On the photosurface of sensor 6.
The directional light that described excitation source 1 sends incides described semi-transparent semi-reflecting lens 2 back reflection to lens 3, Qi Zhongsuo
Stating the angle between directional light and the directional light of reflection of semi-transparent semi-reflecting lens 2 incidence is 45～135 °, preferably 90 °.
Described information process analysis unit uses single-chip microcomputer.
Fig. 2 is the detection side of the immunochromatography detecting system exciting dual frequency reception principle based on single-frequency of the present utility model
Method, comprises the following steps:
1) excitation wavelength of fluorescence spectrum, is determined: utilize ultraviolet/visible spectrophotometer to measure target detection thing 280
～the absorption curve of 760nm, find target detection thing the strongest characteristic absorption peak correspondence peak position, be most preferably exciting of sample
Wavelength；
2), target detection thing is added in test strip；
3), according to step 1) test result, excitation source 1 sends the maximum excitation wavelength directional light spectrum of sample
Successively after semi-transparent semi-reflecting lens 2 reflects and lens 3 focus on, it is irradiated in test strip, in test strips at lens 3 focal point
After target detection thing is stimulated, launching the fluorescence spectrum of multiband, this spectrum, after lens 3, becomes directional light and is transmitted to
Two points of mirrors 4 of side, two frequency range spectrum are divided into both direction spectrum, respectively fluorescence one and fluorescence two by two points of mirrors 4；
4), fluorescence one through first optical filter 7 filter after, first sensor 5 convert optical signals to the signal of telecommunication and obtain
The detection light intensity of main peak, exports on single-chip microcomputer；
5), fluorescence two through second optical filter 8 filter after, the second sensor 6 convert optical signals to the signal of telecommunication and obtain
The detection light intensity of secondary peak, exports on single-chip microcomputer；
6), by the light intensity value that two spectral detection obtain it is normalized calculating, eliminates detection substrate to wherein certain ripple
The certain interference of section, obtains detecting light intensity, then substitutes into calculating target detection concentration value, normalization in the linear equation demarcated
Algorithm expression formula is as follows:
A=α T1+ β T2
Wherein, A be measure light intensity normalization after value of calculation, T1 Yu T2 is the measurement light intensity value of main peak and secondary peak, alpha+beta=
1；α Yu β parameter is that the intensity relation according to spectrogram determines.
Below in conjunction with specific embodiment, this utility model is elaborated.
Use the metallo-chelate synthesis fluorescent microsphere of lanthanide series rare-earth elements europium, as the fluorescent marker of Concentration Testing,
As it is shown on figure 3, the peak of this fluorescent marker excitation source is about 345nm position, this fluorescent marker emission band
Peak have at two, be the peak position of 615nm at one, be the secondary peak position of 590nm at another, peak position light intensity with
Secondary peak position light intensity is than about 4:1 relation；
Assume that the light intensity that 615nm measures is T1, and the light intensity that 590nm measures is T2, then calculate the light intensity value after normalization
For:
A=0.8*T1+0.2*T2；Because 4:1 relation, so α Yu β parameter value is 0.8 and 0.2；Can after the A value obtained
To carry out piecewise linearity demarcation, calibration process is:
1. the metallo-chelate standard sample of concentration known europium is diluted to a series of gradient in proportion, obtain 11 groups known
Concentration C0Standard sample (as shown in table 1)；
The most each standard sample is detected by detecting system of the present utility model, obtains their T10And T20Value, and
Substitute into formula A=α T1+ β T2, obtain the light intensity value A after normalization0(result is as shown in table 1)；
3. by the light intensity value A after normalization0With concentration value C0One_to_one corresponding, obtain standard curve and linear equation (as Fig. 4,
5, shown in 6, in Fig. 4,5,6, abscissa is light intensity value, and vertical coordinate is concentration value, and Fig. 5 is the first six group concentration value C and light intensity value
Function relation figure between A, now concentration value C and light intensity value A exponent function relation: C=6.406e0.004A, Fig. 6 is latter six groups
Function relation figure between concentration value C and light intensity value A, now concentration value C becomes logarithmic relationship with light intensity value A: C=
137.0ln(A)-781.2；
Light intensity value after the initial concentration value of table 1 standard sample and normalization
Detection target detection thing: add in test strip by target detection thing, opens detection system of the present utility model
System, detection obtains T1=425, T2=106, obtains the light intensity value A=0.8*425+0.2*106=361.2 of correspondence after normalization,
In the range of this value is in shown in Fig. 5, therefore this light intensity value A is substituted into the linear equation C=6.406e of Fig. 50.004AMiddle calculating sample
Concentration value, result is about 27.17ng/mL.
Claims (5)
1. excite the immunochromatography detecting system of dual frequency reception principle based on single-frequency, including ultraviolet/visible spectrophotometer, information
Collecting unit and the information process analysis unit being attached thereto,
Described ultraviolet/visible spectrophotometer is for determining the maximum excitation wavelength of target detection thing；
Described information acquisition unit is for carrying out optical scanning to the target detection thing on test strip surface, and it is to target detection
Thing projects irradiating light beam, to receive the reflected fluorescent light of reagent paper, and described reflected fluorescent light is converted to the signal of telecommunication；Described information processing
Analytic unit for carrying out Treatment Analysis to the described signal of telecommunication, thus output detections result, it is characterised in that
Described information acquisition unit includes excitation source (1), semi-transparent semi-reflecting lens (2), lens (3), two points of mirrors (4) and is used for
The first sensor (5) of the light intensity of the fluorescence that detection receives and the second sensor (6)；The directional light that excitation source (1) sends
It is irradiated on the target detection thing on test strip surface after semi-transparent semi-reflecting lens (2) reflection and lens (3) successively, target detection
The fluorescence that thing ejects is again through two points of mirror (4) reflections and transmission after semi-transparent semi-reflecting lens (2) transmission, and wherein, two points of mirrors (4) are anti-
The fluorescence shot out is irradiated on the photosurface of first sensor (5), and the fluorescence that two points of mirrors (4) transmit is irradiated to the second biography
On the photosurface of sensor (6), the outfan of first sensor (5) and the outfan of the second sensor (6) all with described information at
The input of reason analytic unit is connected.
The immunochromatography detecting system exciting dual frequency reception principle based on single-frequency the most according to claim 1, its feature exists
In, the fluorescence that described two points of mirrors (4) reflect is irradiated to the photosurface of described first sensor (5) through the first optical filter (7)
On.
The immunochromatography detecting system exciting dual frequency reception principle based on single-frequency the most according to claim 1, its feature exists
In, the fluorescence that described two points of mirrors (4) transmit is irradiated to the photosurface of described second sensor (6) through the second optical filter (8)
On.
The immunochromatography detecting system exciting dual frequency reception principle based on single-frequency the most according to claim 1, its feature exists
Described semi-transparent semi-reflecting lens (2) back reflection is incided to lens (3), Qi Zhongsuo in, the directional light that described excitation source (1) sends
The angle stated between directional light and the directional light of reflection that semi-transparent semi-reflecting lens (2) is incident is 45～135 °.
The immunochromatography detecting system exciting dual frequency reception principle based on single-frequency the most according to claim 1, its feature exists
In, described information process analysis unit uses single-chip microcomputer.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106018796A (en) * | 2016-07-05 | 2016-10-12 | 扬州千代科技有限公司 | Immunochromatographic detection system based on single-frequency excitation and double-frequency reception principle and detection method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106018796A (en) * | 2016-07-05 | 2016-10-12 | 扬州千代科技有限公司 | Immunochromatographic detection system based on single-frequency excitation and double-frequency reception principle and detection method thereof |
| CN106018796B (en) * | 2016-07-05 | 2017-10-13 | 扬州千代科技有限公司 | A kind of immunochromatography detecting system and its detection method for exciting dual frequency reception principle based on single-frequency |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20161207 Effective date of abandoning: 20171013 |