CN205473753U

CN205473753U - SVF draws and uses kit

Info

Publication number: CN205473753U
Application number: CN201620044115.5U
Authority: CN
Inventors: 成琴芳; 李华
Original assignee: Shanghai Songtong Biotechnology Co Ltd
Current assignee: Shanghai Songtong Biotechnology Co Ltd
Priority date: 2016-01-18
Filing date: 2016-01-18
Publication date: 2016-08-17
Anticipated expiration: 2026-01-18

Abstract

The utility model discloses a SVF draws and uses kit, including interior body, box sleeve, vice box body and outer box. Interior body and box sleeve constitute main box body. Set up first recess on the up end of interior body, set up a centrifuging tube and place that the chamber is placed in chamber, two filters, the chamber is placed to wide -necked bottle and the chamber is placed to a burette on the bottom surface of this first recess, box sleeve comprises top surface, bottom surface and width direction's both sides face, and an arbitrary open surface that makes interior body follow box sleeve's length direction can insert box sleeve's inner chamber, seven first jacks and a second jack are seted up to this box sleeve's top surface, and seven third jacks are seted up to this box sleeve's bottom surface, the second recess is seted up to the up end of vice box body, sets up two reagent bottles and place the chamber on the bottom surface of this second recess, outer box includes end box and the lid that can open, makes the kit keep encapsulated situation. The utility model discloses a SVF draws and uses kit, not only portable instrument and reagent more can make things convenient for clinical staff to draw SVF.

Description

A kind of SVF extraction test kit

Technical field

This utility model relates to a kind of medical kit, is specifically related to a kind of SVF extraction test kit.

Background technology

Fatty tissue mainly by adipose cell, ASCs, vascular endothelial cell, pericyte, fibroblast, Huge chew cell and extracellular matrix composition.In the fatty tissue of every cubic centimeter volume, include 1 × 10⁶Individual Adipose cell, 1 × 10⁶Individual ASCs, 1 × 10⁶Individual vascular endothelial cell and (1～2) × 10⁶Other cells individual (as The cell of blood sources) autologous adipose tissue is organizational project and the research of regenerative medicine and application provides rich Rich substrate/source of human stem cell.Researcher is by the method for enzymic digestion, from abdominoplasty or liposuction Separation and Extraction stromal vascular fraction cell (stromal vascular fraction in the fatty tissue that art is discarded Cells, SVF).SVF is the colony that an adipose-derived stem cells mixes mutually with other cell component, warp Purer adipose-derived substrate/stem cell (adipose-derived can be obtained after crossing external adhere-wall culture Stromal/stemcells, ASCs).In order to cultivate fat stem cell, it is necessary for from fat extracting SVF. But, among prior art, complex for extracting the technique of SVF, need to use relatively multiple instruments ability Complete, be not very convenient for medical personnel.For medical personnel, in experimentation and facing Among bed research, experiment, operation etc. can be had adverse effect on by the instrument carrying all extraction SVF.

Utility model content

The purpose of this utility model is to overcome the defect of prior art to provide a kind of SVF extraction reagent Box, it has not only received all experimental apparatus that may use and reagent among SVF extraction process, and It is easy to carry, more can facilitate clinical staff and technical staff that SVF is extracted.

The purpose of this utility model is achieved in that a kind of SVF extraction test kit, including interior body, box Set, secondary box body and outer box, described interior body and box set composition main cartridge, wherein,

As a solid cuboid in described, its upper surface is offered the first recessed of a four-prism Groove, the bottom surface of this first groove is offered a centrifuge tube placed cavity, two filter placed cavities, one Wide mouthed bottle placed cavity and a dropper placed cavity；

The hollow cuboid that described box set is made up of the two sides of end face, bottom surface and width, this box set The size fit of profile of inner chamber size and described interior body, make the length direction that described interior body overlap from box times An open surface of anticipating can insert the inner chamber of box set；Identical first the inserting of seven diameters is offered on the end face of this box set Hole and diameter are more than the second jack of the first jack；Seven and seven first are offered on the bottom surface of this box set Jack one_to_one corresponding and diameter are less than the first slot 26 of the first jack；

The external width phase that described secondary box body is a solid thin-and-long cuboid and width overlaps with described box With, the second groove of a thin-and-long cuboid is offered in the upper surface of this secondary box body, at the end of this second groove Two reagent bottle placed cavities are offered in tandem on face；

Described outer box includes back box and openable lid, described back box has one by described main cartridge and The containing cavity that secondary box body is all accommodated therein, described lid covers in described back box, makes described test kit keep Sealing state.

Above-mentioned SVF extraction test kit, wherein, can place four centrifuge tubes in described centrifuge tube containing cavity； A filter is placed respectively in two described filter placed cavities；Can place in described dropper placed cavity Four Pasteur's droppers, place a wide mouthed bottle in described wide mouthed bottle placed cavity.

Above-mentioned a kind of SVF extraction test kit, wherein, the capacity of described centrifuge tube is 50ml, described The capacity of wide mouthed bottle is 100ml, and the capacity of four described Pasteur's droppers is 3ml, described filter Mesh size be 40 μm.

Above-mentioned SVF extraction test kit, wherein, divides in two reagent bottle placed cavities on described secondary box body Fang Zhi first reagent bottle and second reagent bottle.

Above-mentioned SVF extraction test kit, wherein, described back box and described lid are connected to that connect or mutual Independent.

SVF extraction test kit of the present utility model has the following characteristics that and makes SVF extract by appropriate design Among process, all experimental apparatus that may use and reagent are placed in the same aspect of test kit, no Only make these instruments be easy to put and carry, more can facilitate clinical staff and technical staff that SVF is entered Row extracts.Additionally reagent rack and interior body are designed to integral structure, it is to avoid the intersection between therapist Pollute.

Accompanying drawing explanation

Fig. 1 is the top view of SVF extraction test kit of the present utility model；

Fig. 2 is the A-A profile in Fig. 1；

Fig. 3 is the top view of the interior body in SVF extraction test kit of the present utility model；

Fig. 4 is the B-B profile in Fig. 3；

Fig. 5 is the longitudinal sectional drawing of the box set in SVF extraction test kit of the present utility model.

Detailed description of the invention

Below in conjunction with accompanying drawing, the utility model is described in further detail.

Refer to Fig. 1 to Fig. 5, SVF extraction test kit of the present utility model, use medical and food stage Other material makes, and radiation sterilization.Test kit includes interior body 1, box set 2, secondary box body 3 and outer box (not shown), interior body 1 and box set 2 composition main cartridge 10.

Interior body 1 is a solid cuboid, and its upper surface is offered the first groove of a four-prism 11, the bottom surface of this first groove 11 is offered 12, two filter placed cavities of a centrifuge tube placed cavity 13,13 ', wide mouthed bottle placed cavity 14 and a dropper placed cavity 15.Wherein, centrifuge tube placed cavity 12 Interior can crouching puts the centrifuge tube that four root volumes are 50ml, and most of body of these four centrifuge tubes is embedded in centrifugal In pipe placed cavity 12, fraction bottle is positioned at the first groove 11；Two filter placed cavities 13,13 ' Crouching the most respectively and put a filter, the mesh size of filter is 40 μm；Wide mouthed bottle placed cavity Sleeping in 14 to put a capacity be the wide mouthed bottle of 100ml, and most of bottle of this wide mouthed bottle is embedded in wide mouthed bottle and puts Putting in chamber 14, fraction bottle is positioned at the first groove 11；Can crouch in dropper placed cavity 15 and put four appearances Amount is Pasteur's dropper of 3ml.

The hollow cuboid that box set 2 is made up of the two sides 23 of end face 21, bottom surface 22 and width, The length of this box set 2 is identical with the length of interior body 1, the inner width of this box set 2 and the width phase of interior body 1 With, make interior body 1 can insert the inner chamber of box set 2 from any one open surface of the length direction of box set 2；Should Seven the first identical jacks 24 of diameter and a diameter is offered more than the first jack 24 on the end face of box set 2 The second jack 25；This box set 2 bottom surface on offer seven with seven the first jack 24 one_to_one corresponding and Diameter is less than the first slot 26 of the first jack 24.Seven the first jacks 24 are divided into three row and arrange, the left side String and middle string are respectively arranged two the first jacks 24, and the right string arranges three the first jacks 24； Second jack 25 is arranged in the bottom of left side string.

Secondary box body 3 is that a solid thin-and-long cuboid and width are identical with the external width of box set 2, should The second groove 31 of a thin-and-long cuboid is offered in the upper surface of secondary box body 3, at this second groove 31 Two reagent bottle placed cavities 32,32 ' are offered in tandem on bottom surface, these two reagent bottle placed cavities 32, Crouch respectively in 32 ' and put first reagent bottle and second reagent bottle, equipped with collagenase in the first reagent bottle, Equipped with normal saline in second reagent bottle.Most of bottle of the first reagent bottle and second reagent bottle is set In the placed cavity 32,32 ' of secondary box body 3, fraction bottle is positioned at the second groove 31.

It is that connect or separate that outer box includes that back box and openable lid, back box and lid are connected to；The end Having containing cavity main cartridge 10 and secondary box body 3 being all accommodated therein on box, lid covers in back box On, make whole test kit keep sealing state.

The layout of main cartridge 10 and secondary box body 3 be with conveniently laying and be extracted as principle and will place instrument and The placed cavity of reagent designs in same aspect, and when carrying out SVF and extracting, operation is very easy.

SVF extraction test kit of the present utility model in use, first opens the lid of outer box, by main cartridge 10 take out in back box, then are released in box set 2 by interior body 1, can be from interior body 1 and secondary box body 3 Required instrument and reagent being taken out from test kit, box set 2 can be used as reagent rack simultaneously, by centrifuge tube with Pasteur's dropper is plugged in the first jack 24 and first slot 26 of box set 2, and wide mouthed bottle is plugged on box set In second jack 25 of 2.

SVF extraction test kit of the present utility model, laying rack when integrating storage and operate with, All experimental apparatus that may use and reagent among SVF extraction process is made to be placed on examination by appropriate design In the same aspect of agent box, not only make these instruments be easy to put and carry, more can facilitate clinical medical and nursing SVF is extracted by personnel and technical staff.Additionally reagent rack and interior body are designed as one, it is to avoid control Cross-contamination between treatment person.

Above example is the most for illustration of the utility model, rather than to restriction of the present utility model, relevant skill The technical staff in art field, in the case of without departing from spirit and scope of the present utility model, it is also possible to make Various conversion or modification, the technical scheme of the most all equivalents also should belong to category of the present utility model, should Limited by each claim.

Claims

1. a SVF extraction test kit, including interior body, box set, secondary box body and outer box, it is characterised in that described interior body and box set composition main cartridge, wherein,

As a solid cuboid in described, its upper surface offered the first groove of a four-prism, the bottom surface of this first groove is offered a centrifuge tube placed cavity, two filter placed cavities, a wide mouthed bottle placed cavity and a dropper placed cavity；

The hollow cuboid that described box set is made up of the two sides of end face, bottom surface and width, the size fit of the profile of inner chamber size and the described interior body of this box set, make any one open surface of the length direction that described interior body overlap from box can insert the inner chamber that box overlaps；Seven the first identical jacks of diameter and diameter the second jack more than the first jack is offered on the end face of this box set；Seven the first slots being less than the first jack with seven the first jack one_to_one corresponding and diameter are offered on the bottom surface of this box set；

Described secondary box body be a solid thin-and-long cuboid and width identical with the external width that described box overlaps, the second groove of a thin-and-long cuboid is offered in the upper surface of this secondary box body, offers two reagent bottle placed cavities on the bottom surface of this second groove in tandem；

Described outer box includes back box and openable lid, and described back box has a containing cavity described main cartridge and secondary box body being all accommodated therein, and described lid covers in described back box, makes described test kit keep sealing state.

SVF extraction test kit the most according to claim 1, it is characterised in that four centrifuge tubes can be placed in described centrifuge tube containing cavity；A filter is placed respectively in two described filter placed cavities；Four Pasteur's droppers can be placed in described dropper placed cavity, in described wide mouthed bottle placed cavity, place a wide mouthed bottle.

A kind of SVF extraction test kit the most according to claim 2, it is characterised in that the capacity of described centrifuge tube is 50ml, and the capacity of described wide mouthed bottle is 100ml, and the capacity of four described Pasteur's droppers is 3ml, and the mesh size of described filter is 40 μm.

SVF extraction test kit the most according to claim 1, it is characterised in that place first reagent bottle and second reagent bottle in two reagent bottle placed cavities on described secondary box body respectively.

SVF extraction test kit the most according to claim 1, it is characterised in that described back box and described lid are connected to that connect or separate.