CN205011759U - This experiment appearance of full -automatic em - Google Patents
This experiment appearance of full -automatic em Download PDFInfo
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- CN205011759U CN205011759U CN201520320290.8U CN201520320290U CN205011759U CN 205011759 U CN205011759 U CN 205011759U CN 201520320290 U CN201520320290 U CN 201520320290U CN 205011759 U CN205011759 U CN 205011759U
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Abstract
The utility model provides a this experiment appearance of full -automatic em, it includes: culture dish conveyor system configures into and transports each predetermined station department with the culture dish, the mixing tank, cigarette appearance sampling device configures into from taking out cigarette appearance in the cigarette appearance test tube and carrying the cigarette appearance of taking out to the mixing tank in, a plurality of micro - injectors configure the corresponding solution that takes out corresponding solution from the corresponding solution storage device and will take out respectively into and carry to the mixing tank in to make multiple solution and cigarette appearance mix in the mixing tank, with mix liquid conveyor, configure into the culture dish be in carry from the mixed liquid that takes out mixed liquid in the mixing tank and will take out when mixing liquid and adding the station coil to the ware of culture dish in. The utility model discloses a this experiment appearance of full -automatic em adds reagent owing to can transport preposition department with the culture dish voluntarily in the tendency mixing tank to reagent after will mixing makes this experiment of em realize the automation in carrying the culture dish, has improved experimental efficiency and experiment precision.
Description
Technical field
The utility model relates to Ames test technical field, particularly relates to a kind of full-automatic Ames test instrument.
Background technology
B.N.Ames etc., through the effort of more than ten years, set up the salmonella reverse mutation assay (also known as Salmonella reversion test, Ames test) that also development is perfect and are widely adopted by countries in the world in 1975.This method than faster, easy, responsive, economical, and be applicable to test mixing thing, reflect the combined effect of multiple pollutant.The mutagenicity of foodstuff additive, makeup etc. can be detected with Salmonella reversion test, infer its carinogenicity thus; Detect the mutagenicity of Source water and drinking water with Salmonella reversion test, explore the disinfectant measure compared with current methods hygienic safety more; Or detect the mutagenicity of municipal effluent and trade effluent, in conjunction with chemical analysis, follow the trail of source of pollution, for research Preventing Countermeasures provides foundation; Detect the mutagenicity of soil, mud, industrial residue compost, refuse ashes, after polluting by mutagen to prevent the soil maintaining life, by the murrain mankind; Detect the mutagenicity of gaseous pollutant, preventing pollution thing via air, by breathing human body generation potential hazard; With Salmonella reversion test research compound structure with cause the relation of sex change, for synthesis provides theoretical foundation to environment without the new compound of potential hazard; Detect the mutagenicity of agricultural chemicals before and after microbiological deterioration, understand agricultural chemicals after application in metabolic process to the mankind with or without hidden danger; Also useful Salmonella reversion test screening anti-mutation thing, researchs and develops new anticarcinogen etc.Existing Ames test is all that experimenter adopts dropper to add reagent in culture dish, and level of automation is low, and cost of labor is high, although can ensure certain experimental precision, experimental result deviation also can be caused huge due to the error of people.
Utility model content
At least one object of the present utility model to provide a kind of full-automatic Ames test instrument, and the level of automation, the cost of labor that significantly improve experiment are low, and experimental precision is high.
To achieve these goals, the utility model provides a kind of full-automatic Ames test instrument, and it comprises:
Culture dish haulage system, is configured to culture dish is transported to each predetermined station place;
Tempering tank;
Cigarette sample sampling unit, is configured to from cigarette sample test tube, take out cigarette sample and be delivered in described tempering tank by the cigarette sample of taking-up;
Multiple microsyringe, is configured to respectively take out corresponding solution from corresponding solution storage device and is delivered in described tempering tank by the corresponding solution taken out, mixing to make multiple solution and described cigarette sample in described tempering tank; With
Mixed solution e Foerderanlage, is configured to be in when mixed solution adds station at described culture dish from described tempering tank, take out mixed solution and be delivered in the ware dish of described culture dish by the mixed solution of taking-up.
Alternatively, described full-automatic Ames test instrument also comprises: opening-closing cover device, it hoisting appliance comprising vacuum pad and drive described vacuum pad to move up and down, and is configured to:
Before described mixed solution e Foerderanlage carries mixed solution in the ware dish of described culture dish, the ware lid of described culture dish is made to depart from described ware dish, to carry mixed solution in described ware dish;
And after described mixed solution e Foerderanlage carries mixed solution in described ware dish, make described ware lid be covered on described ware dish.
Alternatively, described culture dish haulage system comprises:
Transverse motion mechanism, is configured to described culture dish is transversely moved in direction; With
Longitudinal motion mechanism, is installed on described transverse motion mechanism, and transversely to move in direction with described culture dish, it has the accommodation section for placing described culture dish, is configured to described culture dish is moved along the longitudinal direction.
Alternatively, described transverse motion mechanism comprises:
Cross slide way, is arranged on the base of full-automatic Ames test instrument;
Transverse slider, is slidably mounted on described cross slide way; With
Travelling belt, is configured to drive described transverse slider to move along described cross slide way;
Described longitudinal motion mechanism comprises:
Longitudinal rail, is fixed on described transverse slider, transversely to move in direction with described transverse slider;
There is the chassis of described accommodation section, be slidably mounted on described longitudinal rail, be configured to place described culture dish; With
Lead screw transmission mechanism, is configured to drive described chassis to move along described longitudinal rail.
Alternatively, described multiple microsyringe and described mixed solution e Foerderanlage are installed on the top of described cross slide way along the length direction compartment of terrain of described cross slide way.
Alternatively, described culture dish haulage system also comprises:
Two culture dish sorting stack devices, each described culture dish sorting stack device comprises:
Clamp assemblies, it has two V-type holders be oppositely arranged; With
Ware holder, vertically can be arranged at the below of described two V-type holders be oppositely arranged movably; And
Described in one of them, culture dish sorting stack device is configured to the culture dish of the foot in vertically place one group of culture dish to be placed on the chassis of described culture dish haulage system; Culture dish stack device described in another is configured to the culture dish on the chassis of described culture dish haulage system to be stacked to the below of the culture dish of the foot in the one group of culture dish vertically placed.
Alternatively, each described microsyringe comprise the first ram pump, the first T-valve, solution inlet pipe, solution go out pipe and solution intervalve, wherein
The entrance of described solution inlet pipe is configured to suck corresponding solution from corresponding solution storage device, and the outlet of described solution inlet pipe is connected to a valve port of described first T-valve; Described solution intervalve is for connecting another valve port of described first ram pump and described first T-valve; The import that described solution goes out pipe is connected to the another valve port of described first T-valve, and the outlet that described solution goes out pipe is for described tempering tank conveying liquid.
Alternatively, described cigarette sample sampling unit comprises: the second ram pump, on-off valve, scavenging solution inlet pipe, cigarette sample intervalve and cigarette sample inlet/outlet pipe and rotating lifting gear, wherein
Described cigarette sample intervalve is for connecting a valve port of described second ram pump and described on-off valve; One end of described cigarette sample inlet/outlet pipe is connected to another valve port of described on-off valve, described rotating lifting gear is configured to the other end of described cigarette sample inlet/outlet pipe to insert in described cigarette sample test tube to promote the other end of described cigarette sample inlet/outlet pipe after the cigarette sample sucking predetermined amount under the effect of described second ram pump, and make the other end of described cigarette sample inlet/outlet pipe be put to described cigarette sample test tube outer after turn to above described tempering tank, enter under the effect of described second ram pump in described tempering tank to make the cigarette sample of described predetermined amount;
The entrance of described scavenging solution inlet pipe is configured to suck cleaning solution from scavenging solution casing, and the outlet of described scavenging solution inlet pipe is connected to the secondary inlet of described second ram pump via valve.
Alternatively, described mixed solution e Foerderanlage comprises mixing inlet pipe, peristaltic pump and blends pipe and vertical lifting device;
Described vertical lifting device is configured to after carry mixed solution in described culture dish, the end blending pipe described in making is moved downward in waste liquid tank, to be delivered in waste liquid tank by the cleaning solution in described tempering tank, thus pipeline is utilized to flow to waste collecting device;
Described mixed solution e Foerderanlage be also configured to utilize described in blend pipe from be in mixed solution add station described culture dish ware dish in aspirate mixed solution, and the ware dish mixed solution of suction told to this culture dish, mix to make the culture dish in the ware dish of this culture dish.
Alternatively, the quantity of described cigarette sample test tube is 10, to place 10 kinds of cigarette samples; The quantity of described multiple microsyringe is 5, supplies liquid, bacterium liquid, solvent, S9 and top layer glue to carry S9 respectively.
At least there is following technique effect in the utility model embodiment:
1) full-automatic Ames test instrument of the present utility model is owing to automatically can be transported to preposition place by culture dish, reagent is added in trend tempering tank, and mixed reagent is transported in culture dish, Ames test is made to achieve automatization, on the basis reducing cost of labor, improve conventional efficient and experimental precision.
2) full-automatic Ames test instrument of the present utility model owing to can add reagent in each culture dish in one group of culture dish, and the culture dish after adding reagent is piled up into one group, further increases conventional efficient and experiment convenience.
3) full-automatic Ames test instrument of the present utility model is owing to can mix the cigarette sample of multiple different concns with all ingredients, and making to carry out the experiment of many groups in the same apparatus becomes possibility, and is convenient to the contrast of experimental result.
4) full-automatic Ames test instrument of the present utility model is owing to completely automatically can complete Ames test in an aseptic environment, and adopt 6 groups of independent high precision micro ceramic pumps (ram pump) controlled to complete pipetting, avoid the crossed contamination that in the pollution and test brought due to external environment, human factor causes, thus improve accuracy and the reliability of experimental result.
5) full automatic Ames test instrument of the present utility model is owing to requiring to carry out separator to each experiment culture dish according to the experimental program of experimenter's customization, experimenter is identified easily and distinguishes culture dish corresponding to each group of experiment, greatly reduce the workload of experimenter and improve the convenience of experimental result.
6) full-automatic Ames test instrument of the present utility model carries out heating in water bath insulation (Ames test top layer glue solution) and ice bath refrigerating thermal-insulation (Ames test S9 solution) owing to can adopt independent temperature system to the relevant secondary solvent of experiment, make the character of relevant secondary solvent can remain stable, further reduce experimental error, improve the accuracy of experiment.
7) full-automatic Ames test instrument of the present utility model is owing to can adopting advanced mixing and shaking up technology, experiment is fully mixed with various solution, and evenly tiling, on culture dish, makes experiment more press close to ideal model, further increases accuracy and the reliability of experiment.
Accompanying drawing explanation
Hereinafter describe specific embodiments more of the present utility model with reference to the accompanying drawings by way of example, and not by way of limitation in detail.Reference numeral identical in accompanying drawing denotes same or similar parts or part.It should be appreciated by those skilled in the art that these accompanying drawings may not be drawn in proportion.In accompanying drawing:
Fig. 1 is the one-piece construction figure of the full-automatic Ames test instrument according to the utility model embodiment;
Fig. 2 is the local structural graph of the full-automatic Ames test instrument according to the utility model embodiment;
Fig. 3 is the schematic expanded view at the place of A shown in Fig. 2;
Fig. 4 is the local structural graph of the full-automatic Ames test instrument according to another embodiment of the utility model;
Fig. 5 is the schematic expanded view at the place of B shown in Fig. 4;
Fig. 6 is the schematic diagram of the full-automatic Ames test Yi Zhong culture dish haulage system according to the utility model embodiment;
Fig. 7 is the schematic diagram of culture dish sorting stack device in the culture dish haulage system according to the utility model embodiment.
Embodiment
According to hereafter by reference to the accompanying drawings to the detailed description of the utility model specific embodiment, those skilled in the art will understand above-mentioned and other objects, advantage and feature of the present utility model more.
Fig. 1 is the one-piece construction figure of the full-automatic Ames test instrument according to the utility model embodiment.As shown in Figure 1, and referring to figs. 2 and 3.The utility model embodiment provides a kind of full-automatic Ames test instrument, and it makes Ames test achieve automatization, on the basis reducing cost of labor, improves conventional efficient and experimental precision.This full-automatic Ames test instrument can comprise culture dish haulage system 20, tempering tank 30, cigarette sample test tube 41, cigarette sample sampling unit 40, multiple solution storage device 51 and multiple microsyringe 50 and mixed solution e Foerderanlage 60.
Particularly, in the utility model embodiment, it should be noted that some pipelines are only illustrated portions in the example shown, some pipelines are not illustrated in the drawings.Culture dish haulage system 20 is configured to culture dish is transported to each predetermined station place, and it is transported to mixed solution to major general's culture dish and adds station, takes away culture dish to next station after treating to be added mixed solution in culture dish.Such as, culture dish haulage system 20 receives culture dish at its initial station, and culture dish is transported to the mixed solution interpolation station adding mixed solution from its initial station, and then this culture dish is transported to end station, to make this culture dish removed; And then get back to initial station and receive next culture dish.
Cigarette sample sampling unit 40 is configured to from cigarette sample test tube 41, take out cigarette sample and be delivered in tempering tank 30 by the cigarette sample of taking-up.In an embodiment of the present utility model, cigarette sample sampling unit 40 can extract the cigarette sample of predetermined amount from cigarette sample test tube 41, and spues the cigarette sample of this predetermined amount in tempering tank 30.Such as, cigarette sample sampling unit 40 can comprise: the second ram pump 45, on-off valve 42, scavenging solution inlet pipe (not shown), cigarette sample intervalve (not shown) and cigarette sample inlet/outlet pipe 43 and rotating lifting gear 44, and wherein cigarette sample intervalve is for connecting a valve port of the second ram pump 45 and on-off valve 42, one end (being called the first end of cigarette sample inlet/outlet pipe hereinafter) of cigarette sample inlet/outlet pipe is connected to another valve port of on-off valve 42, rotating lifting gear 44 is configured to the other end of cigarette sample inlet/outlet pipe (being called the second end of cigarette sample inlet/outlet pipe hereinafter) to insert in cigarette sample test tube 41 to promote the other end of cigarette sample inlet/outlet pipe after the cigarette sample sucking predetermined amount under the effect of the second ram pump 45, and make the other end of cigarette sample inlet/outlet pipe be put to cigarette sample test tube 41 outer after turn to above tempering tank 30, enter in tempering tank 30 under the effect of the second ram pump 45 to make the cigarette sample of predetermined amount.Cigarette sample intervalve can extend along spiral-line.The entrance of scavenging solution inlet pipe is configured to suck cleaning solution from scavenging solution casing, and the outlet of scavenging solution inlet pipe is connected to the secondary inlet of the second ram pump via valve.In embodiments more of the present utility model, on-off valve 42 can be the second T-valve, and described cigarette sample intervalve is for connecting a valve port of described second ram pump and described on-off valve; One end of described cigarette sample inlet/outlet pipe is connected to another valve port of described on-off valve, all the other port closeds of described second T-valve.
The special construction of above-mentioned cigarette sample sampling unit 40 can allow to carry out Ames test to multiple cigarette sample simultaneously, and the quantity of cigarette sample test tube 41 can be 10, to place 10 kinds of cigarette samples.When testing with the first cigarette sample, second end of cigarette sample inlet/outlet pipe 43 is turned to the top of the first cigarette sample test tube by rotating lifting gear 44, then the below in its invisible spectro first cigarette sample liquid face of insertion first cigarette sample that declines is made, control on-off valve 42 and be communicated with cigarette sample intervalve and cigarette sample inlet/outlet pipe 43, second ram pump 45 air-breathing to make to suck in cigarette sample inlet/outlet pipe 43 first cigarette sample of predetermined amount.Rotating lifting gear 44, by the top of the second end band to the first cigarette sample test tube of cigarette sample inlet/outlet pipe 43, then makes it turn to the top of tempering tank 30, and makes it drop to predetermined position.Second ram pump 45 feels elated and exultant that the first cigarette sample of the predetermined amount sucked in cigarette sample inlet/outlet pipe 43 is spued in tempering tank 30.
After in the ware dish mixed solution in tempering tank 30 being delivered to culture dish, if desired cigarette sample (be that second cigarette sample test as changed the first cigarette sample) is changed, then disconnect cigarette sample intervalve and cigarette sample inlet/outlet pipe, open the secondary inlet of the second ram pump, second ram pump 45 air-breathing enters in the second ram pump to make cleaning solution through scavenging solution inlet pipe, connect cigarette sample intervalve and cigarette sample inlet/outlet pipe, close the secondary inlet of the second ram pump, second ram pump 45 is felt elated and exultant to be spued by the cleaning solution in it in tempering tank 30, to clean cigarette sample inlet/outlet pipe, the cleaning solution entered in tempering tank 30 cleans tempering tank 30, mixed solution e Foerderanlage 60 sucks cleaning solution and transfers out in tempering tank 30, to clean the transfer line of mixed solution e Foerderanlage 60.In embodiments more of the present utility model, suck in advance in the pump housing of the second ram pump 45 and in cigarette sample intervalve and store some scavenging solutions, namely the second ram pump 45 first sucks scavenging solution, then control on-off valve 42 and be communicated with cigarette sample intervalve and cigarette sample inlet/outlet pipe, when needs clean cigarette sample inlet/outlet pipe, second ram pump 45 directly exports through cigarette sample inlet/outlet pipe the scavenging solution stored in its cavity and in cigarette sample intervalve to tempering tank 30, realizes the cleaning to cigarette sample inlet/outlet pipe.When needs wash cigarette sample, owing to having air insulated post between the cigarette sample in cigarette sample inlet/outlet pipe and cleaning liquid, therefore scavenging solution can not impact cigarette sample.
When testing with the second cigarette sample, second end of cigarette sample inlet/outlet pipe 43 is turned to the top of the second cigarette sample test tube by rotating lifting gear 44, then the below in its invisible spectro second cigarette sample liquid face of insertion second cigarette sample that declines is made, control on-off valve 42 and be communicated with cigarette sample intervalve and cigarette sample inlet/outlet pipe 43, second ram pump 45 air-breathing to make to suck in cigarette sample inlet/outlet pipe 43 second cigarette sample of predetermined amount.Rotating lifting gear 44, by the top of the second end band to the second cigarette sample test tube 41 of cigarette sample inlet/outlet pipe 43, then makes it turn to the top of tempering tank 30, and makes it drop to predetermined position.Second ram pump 45 feels elated and exultant that the second cigarette sample of the predetermined amount sucked in cigarette sample inlet/outlet pipe 43 is spued in tempering tank 30.
Multiple microsyringe 50 is configured to respectively take out corresponding solution from corresponding solution storage device 51 and is delivered in tempering tank 30 by the corresponding solution taken out, and mixes in tempering tank 30 to make multiple solution and cigarette sample.The quantity of multiple microsyringe 50 can be 5, supplies liquid, bacterium liquid, solvent, S9 and top layer glue to carry S9 respectively.Top layer glue also can be called as upper strata soft agar.S9 also can be called as S9 mixed solution.When it should be noted that experiment, the bottom glue of experiment has prepared in advance and has been laid in the ware tray bottom of culture dish.In embodiments more of the present utility model, each microsyringe 50 comprises the first ram pump 55, first T-valve 52, solution inlet pipe 53, solution go out pipe 54 and solution intervalve 56, wherein the entrance of solution inlet pipe 53 is configured to suck corresponding solution from corresponding solution storage device 51, and the outlet of solution inlet pipe 53 is connected to a valve port of the first T-valve 52; Solution intervalve 56 is for connecting another valve port of the first ram pump 55 and the first T-valve 52; The import that solution goes out pipe 54 is connected to the another valve port of the first T-valve 52, and solution goes out the outlet of pipe 54 for carrying liquid to tempering tank 30.Solution intervalve 56 can extend along spiral-line, to increase volume, prevents solution to be inhaled in the first ram pump.The end that the solution of multiple microsyringe 50 goes out pipe 54 is fixed on a fixed block, and this fixed block is formed with groove, turns to the position above tempering tank 30 with the second end limiting cigarette sample inlet/outlet pipe 43.
Each microsyringe also can comprise scavenging solution inlet pipe, and its entrance is configured to suck cleaning solution from scavenging solution casing, and the outlet of scavenging solution inlet pipe is connected to the secondary inlet of the first ram pump via valve.
When each microsyringe 50 works, control the first T-valve 52 and be communicated with solution inlet pipe 53 and solution intervalve 56, first ram pump 55 air-breathing is to make the corresponding solution sucking predetermined amount in solution inlet pipe 53, wherein the part solution of predetermined amount enters in solution intervalve 56, control the first T-valve 52 again and be communicated with solution intervalve 56 and solution goes out pipe 54, second ram pump 55 feels elated and exultant to make the solution sucked in solution intervalve 56 spue in tempering tank 30, to mix with cigarette sample.When needs clean, open the secondary inlet of the first ram pump, disconnection solution intervalve 56 and solution inlet pipe 53, solution go out pipe 54, first ram pump 55 air-breathing enters in the first ram pump 55 to make cleaning solution through scavenging solution inlet pipe, close the secondary inlet of the first ram pump, connection solution intervalve 56 and solution inlet pipe 53, first ram pump 55 are felt elated and exultant to be spued by the cleaning solution in it in corresponding solution storage device 51, to clean solution inlet pipe 53.Then, connection solution intervalve 56 and solution go out pipe 54, and the first ram pump 55 is felt elated and exultant to be spued by the cleaning solution in it in tempering tank 30, cleans to go out pipe 54 to solution.
Mixed solution e Foerderanlage 60 is configured to be in when mixed solution adds station at culture dish from tempering tank 30, take out mixed solution and be delivered in the ware dish of culture dish by the mixed solution of taking-up.Preferably, mixed solution e Foerderanlage 60 be also configured to utilize described in blend pipe from be in mixed solution add station described culture dish ware dish in aspirate mixed solution, and the ware dish mixed solution of suction told to this culture dish, mix to make the culture dish in the ware dish of this culture dish.Mixed solution e Foerderanlage 60 comprises mixing inlet pipe 61, peristaltic pump 62 and blends pipe 63 and vertical lifting device 64.Vertical lifting device 64 is configured to after carry mixed solution in culture dish, makes the end blending pipe 63 be moved downward in waste liquid tank, to be delivered in waste liquid tank by the cleaning solution in tempering tank 30, thus utilizes pipeline to flow to waste collecting device.In the utility model embodiment, waste liquid tank is positioned at and is in the below that mixed solution adds the culture dish of station, waste liquid tank receives from the unnecessary mixed solution blended in pipe with from the cleaning solution blending pipe, and utilizes pipeline to make unnecessary mixed solution and cleaning solution flow out full-automatic Ames test instrument.
Because culture dish comprises ware dish and ware lid, therefore, full-automatic Ames test instrument in the utility model embodiment also comprises opening-closing cover device 70, it is configured to: carry mixed solution in the ware dish of mixed solution e Foerderanlage 60 to culture dish before, the ware lid of culture dish is made to depart from ware dish, to carry mixed solution in ware dish; And after mixed solution e Foerderanlage 60 carries mixed solution in ware dish, make ware lid be covered on ware dish.Particularly, opening-closing cover device can comprise vacuum pad 72 and the hoisting appliance 71 driving vacuum pad to move up and down and pump.When uncapping, vacuum pad 72 moves down, and after the air after extruding the air in it or in pump aspiration vacuum sucker 72, hoisting appliance 71 drives ware lid to move upward.During closing lid, vacuum pad 72 drives ware lid to move downward, and after ware lid is covered on ware dish, culture dish moves, thus vacuum pad and ware lid are departed from; Or after ware lid is covered on ware dish, pump carries air in vacuum pad 72, makes ware lid and vacuum pad depart from.
In embodiments more of the present utility model, as shown in Figures 4 to 6, culture dish haulage system 20 comprises transverse motion mechanism, be configured to culture dish is transversely moved in direction, so that culture dish is transferred to other one from one of full-automatic Ames test instrument, mixed solution adds station can be positioned at a certain position in the middle of full-automatic Ames test instrument.
In order to make the layout of full-automatic Ames test instrument more rationally and be convenient to mixed solution e Foerderanlage 60 to blend waste liquid in pipe or cleaning fluid discharge in the waste liquid tank on base 10, culture dish haulage system 20 also comprises longitudinal motion mechanism, be installed on transverse motion mechanism, transversely to move in direction with culture dish, it has the accommodation section for culture dish holding, is configured to culture dish is moved along the longitudinal direction.Particularly, culture dish is after from starting position, transversely predetermined distance is moved in direction, move predetermined distance more along the longitudinal direction and reach mixed solution interpolation station, cover opening-closing mechanism 70 takes ware lid away, the mixed solution of taking-up is delivered in the ware dish of culture dish by mixed solution e Foerderanlage 60, and then cover opening-closing mechanism 70 covers ware lid.After culture dish returns predetermined distance along the longitudinal direction, more transversely direction moves to end station.Be laid on the ware dish of culture dish to make mixed solution, by the transverse shifting of turning back with vertically move, such as, the culture dish in it with mixed solution can move back and forth twice along the longitudinal direction, and then transversely direction moves back and forth twice, be finally parked in end station.Realized by horizontal and vertical running gear, make mixed solution be laid on the ware dish of culture dish, this process also can be claimed to be shake up process to mixed solution.After culture dish returns predetermined distance along the longitudinal direction, mixed solution e Foerderanlage 60 carries waste liquid or cleaning solution in waste liquid tank.
Particularly, as shown in Figure 6, transverse motion mechanism comprises cross slide way 21, is arranged on the base 10 of full-automatic Ames test instrument; Transverse slider, is slidably mounted on cross slide way 21; And travelling belt, be configured to drive transverse slider transversely guide rail move.Longitudinal motion mechanism can comprise: longitudinal rail, is fixed on transverse slider, transversely to move in direction with transverse slider; There is the chassis 22 of accommodation section, be slidably mounted on longitudinal rail, be configured to culture dish holding; And lead screw transmission mechanism, be configured to drive chassis 22 longitudinally guide rail move.
The length direction compartment of terrain of mixed solution e Foerderanlage 60, multiple microsyringe 50 and mixed solution e Foerderanlage 60 transversely guide rail is installed on the top of cross slide way.Tempering tank 30, cigarette sample test tube 41, cigarette sample sampling unit 40 and multiple solution storage device 51 can be arranged on base 10, and are positioned at the front of cross slide way.
In other embodiment of the present utility model, the culture dish haulage system of full-automatic Ames test instrument also comprises two culture dish sorting stack devices 80, each culture dish sorting stack device 80 is configured to the culture dish of the foot in vertically place one group of culture dish to be placed on the chassis 22 of culture dish haulage system 20, or the culture dish on the chassis 22 of culture dish haulage system 20 is stacked to the below of culture dish of the foot in one group of culture dish vertically placing.Two culture dish sorting stack devices 80 can be arranged on cross slide way 21 two ends, the culture dish of the foot in one group of culture dish to be placed into the chassis 22 of culture dish haulage system 20, with the below of the culture dish of the foot be stacked to by the culture dish on the chassis 22 of culture dish haulage system 20 in one group of culture dish vertically placing, thus automatically complete picking and placeing of culture dish.
In a specific embodiment of the present utility model, as shown in Figure 7, culture dish sorting stack device 80 comprises clamping device 81 and can the ware holder 82 of vertically movement, ware holder 82 can be driven vertically to move by hoisting appliance 84.Clamping device 81 and ware holder 82 are configured to: hold one group of culture dish in ware holder 82, and clamping device 81 is when clamping in one group of culture dish, with the ware lid overlying contact of foot ware dish, ware holder 82 moves down until the diapire of this culture dish contacts with chassis 22 with the culture dish of the foot in one group of culture dish, thus is placed on chassis 22 by the culture dish of the foot in vertically place one group of culture dish; Wherein, the accommodation section on chassis 22 is the groove extended along the length direction of described ware holder, and to make the diapire of culture dish contact with chassis 22, and when the direction 22 surpassed on chassis away from ware holder is moved, ware holder is extracted out from groove.The ware dish of the foot in one group of culture dish is clamped at clamping device 81, ware holder 82 is positioned at predetermined position to allow the groove inserted when chassis 22 is moved near ware holder on chassis 22, and when groove is inserted in ware holder 82, ware holder 82 culture dish carried on it moves upward until the ware lid of the culture dish carried contacts with the ware dish of the foot in one group of culture dish, the culture dish of the foot in new one group of culture dish is become with the culture dish making ware holder 82 carry, clamping device 81 unclamps clamping, ware holder 82 continues to move upward to make clamping device 81 clamp the culture dish of the foot in one group of new culture dish, thus the culture dish on chassis 22 is stacked to the below of the culture dish of the foot in the one group of culture dish vertically placed, to become one group of new culture dish.Such as, clamping device 81 comprises two V-type holders be oppositely arranged and two longitudinal directions clamp guide rails and grasping drive unit 85, two V-type holders be oppositely arranged clamp guide rail move toward one another to clamp the ware dish of corresponding culture dish along corresponding longitudinal direction under the effect of grasping drive unit 85, and two V-type holders be oppositely arranged unclamp along opposing the moving of corresponding longitudinally clamping guide rail the ware dish clamping corresponding culture dish under the effect of grasping drive unit 85.
Two culture dish sorting stack devices 80 lay respectively at the two ends of cross slide way 21, culture dish sorting stack device is used for the chassis 22 culture dish of the foot in vertically place one group of culture dish being placed into culture dish haulage system 20, and another one culture dish sorting stack device is used for the culture dish on the chassis 22 of culture dish haulage system 20 to be stacked to the below of the culture dish of the foot in the one group of culture dish vertically placed.In the utility model embodiment, each culture dish is placed 80 devices and is also comprised curved baffle 83, so that the placement of one group of culture dish, ensures the stability of one group of culture dish.
In other embodiment of the present utility model, full-automatic Ames test instrument also comprises at least one magnetic stirring apparatus, at least one thermostat and spray code spraying apparatus 90.Each magnetic stirring apparatus is configured to make the solution in a solution storage device 51 produce concussion, to prevent precipitation.Each thermostat is configured to make the solution in a solution storage device 51 remain on steady temperature.The quantity of magnetic stirring apparatus can be 2, is respectively used to stir upper strata soft agar and S-9 mixed solution.The quantity of thermostat is 2, and not for being incubated upper strata soft agar and S-9 mixed solution, the thermostat wherein preserving S-9 mixed solution is 4 DEG C of refrigeration plants, is specially semiconductor refrigerator; The thermostat preserving upper strata soft agar is 45 DEG C of heating units, is specially water bath device.Spray code spraying apparatus is configured to carry out coding to culture dish, to distinguish culture dish.
Particularly, the working process of the utility model embodiment is: be placed on by one group of culture dish in the ware holder 82 of culture dish sorting stack device 80 of the left side of full-automatic Ames test instrument, adjustment makes ware holder 82 hold one group of culture dish, and clamping device 81 clamps in one group of culture dish, with the ware dish of the ware lid overlying contact of foot, ware holder 82 moves down, the culture dish of bottom in one group of culture dish is placed on chassis 22, predetermined distance is moved on travelling belt drive chassis 22 transversely guide rail, opening-closing cover device 70 takes away ware lid, lead screw transmission mechanism drives chassis 22 longitudinally guide rail reach predetermined distance, make culture dish be in mixed solution and add station.
Cigarette sample sampling unit 40 and 5 microsyringes 50 add cigarette sample or corresponding solution according to predetermined test order in tempering tank 30.Mixed mixed solution is transported in the ware dish of culture dish by mixed solution e Foerderanlage 60.Lead screw transmission mechanism drives chassis 22 longitudinally to move predetermined distance after guide rail, makes culture dish be in the top of cross slide way.Opening-closing cover device 70 covers ware lid.Travelling belt drive chassis 22 transversely guide rail moves to the culture dish sorting stack device 80 of the right side of full-automatic Ames test instrument, makes clamping device 81 clamp this culture dish.And travelling belt drive chassis 22 transversely guide rail move in the process of culture dish sorting stack device of the right side of full-automatic Ames test instrument, use spray code spraying apparatus 90 pairs of culture dish to carry out coding.Successively by after adding mixed solution in ware dish each in one group of culture dish, that takes right side away completing pile (one group) culture dish adding mixed solution, completes.
After completing add mixed solution in the ware dish of a culture dish, can clean mixed solution e Foerderanlage 60, cigarette sample sampling unit 40 and tempering tank 30.
The above is only preferred implementation of the present utility model; should be understood that; for those skilled in the art, under the prerequisite not departing from the utility model principle, any improvements and modifications made also should be considered as protection domain of the present utility model.
Claims (10)
1. a full-automatic Ames test instrument, is characterized in that, comprising:
Culture dish haulage system, is configured to culture dish is transported to each predetermined station place;
Tempering tank;
Cigarette sample sampling unit, is configured to from cigarette sample test tube, take out cigarette sample and be delivered in described tempering tank by the cigarette sample of taking-up;
Multiple microsyringe, is configured to respectively take out corresponding solution from corresponding solution storage device and is delivered in described tempering tank by the corresponding solution taken out, mixing to make multiple solution and described cigarette sample in described tempering tank; With
Mixed solution e Foerderanlage, is configured to be in when mixed solution adds station at described culture dish from described tempering tank, take out mixed solution and be delivered in the ware dish of described culture dish by the mixed solution of taking-up.
2. full-automatic Ames test instrument according to claim 1, is characterized in that, also comprise:
Opening-closing cover device, it hoisting appliance comprising vacuum pad and drive described vacuum pad to move up and down, is configured to:
Before described mixed solution e Foerderanlage carries mixed solution in the ware dish of described culture dish, the ware lid of described culture dish is made to depart from described ware dish, to carry mixed solution in described ware dish;
And after described mixed solution e Foerderanlage carries mixed solution in described ware dish, make described ware lid be covered on described ware dish.
3. full-automatic Ames test instrument according to claim 1, is characterized in that, described culture dish haulage system comprises:
Transverse motion mechanism, is configured to described culture dish is transversely moved in direction; With
Longitudinal motion mechanism, is installed on described transverse motion mechanism, and transversely to move in direction with described culture dish, it has the accommodation section for placing described culture dish, is configured to described culture dish is moved along the longitudinal direction.
4. full-automatic Ames test instrument according to claim 3, is characterized in that,
Described transverse motion mechanism comprises:
Cross slide way, is arranged on the base of full-automatic Ames test instrument;
Transverse slider, is slidably mounted on described cross slide way; With
Travelling belt, is configured to drive described transverse slider to move along described cross slide way;
Described longitudinal motion mechanism comprises:
Longitudinal rail, is fixed on described transverse slider, transversely to move in direction with described transverse slider;
There is the chassis of described accommodation section, be slidably mounted on described longitudinal rail, be configured to place described culture dish; With
Lead screw transmission mechanism, is configured to drive described chassis to move along described longitudinal rail.
5. full-automatic Ames test instrument according to claim 4, is characterized in that, described multiple microsyringe and described mixed solution e Foerderanlage are installed on the top of described cross slide way along the length direction compartment of terrain of described cross slide way.
6. full-automatic Ames test instrument according to claim 1, is characterized in that, described culture dish haulage system also comprises:
Two culture dish sorting stack devices, each described culture dish sorting stack device comprises:
Clamp assemblies, it has two V-type holders be oppositely arranged; With
Ware holder, vertically can be arranged at the below of described two V-type holders be oppositely arranged movably; And
Described in one of them, culture dish sorting stack device is configured to the culture dish of the foot in vertically place one group of culture dish to be placed on the chassis of described culture dish haulage system; Culture dish stack device described in another is configured to the culture dish on the chassis of described culture dish haulage system to be stacked to the below of the culture dish of the foot in the one group of culture dish vertically placed.
7. full-automatic Ames test instrument according to claim 1, is characterized in that, each described microsyringe comprise the first ram pump, the first T-valve, solution inlet pipe, solution go out pipe and solution intervalve, wherein
The entrance of described solution inlet pipe is configured to suck corresponding solution from corresponding solution storage device, and the outlet of described solution inlet pipe is connected to a valve port of described first T-valve; Described solution intervalve is for connecting another valve port of described first ram pump and described first T-valve; The import that described solution goes out pipe is connected to the another valve port of described first T-valve, and the outlet that described solution goes out pipe is for described tempering tank conveying liquid.
8. full-automatic Ames test instrument according to claim 1, is characterized in that, described cigarette sample sampling unit comprises: the second ram pump, on-off valve, scavenging solution inlet pipe, cigarette sample intervalve and cigarette sample inlet/outlet pipe and rotating lifting gear, wherein
Described cigarette sample intervalve is for connecting a valve port of described second ram pump and described on-off valve; One end of described cigarette sample inlet/outlet pipe is connected to another valve port of described on-off valve, described rotating lifting gear is configured to the other end of described cigarette sample inlet/outlet pipe to insert in described cigarette sample test tube to promote the other end of described cigarette sample inlet/outlet pipe after the cigarette sample sucking predetermined amount under the effect of described second ram pump, and make the other end of described cigarette sample inlet/outlet pipe be put to described cigarette sample test tube outer after turn to above described tempering tank, enter under the effect of described second ram pump in described tempering tank to make the cigarette sample of described predetermined amount;
The entrance of described scavenging solution inlet pipe is configured to suck cleaning solution from scavenging solution casing, and the outlet of described scavenging solution inlet pipe is connected to the secondary inlet of described second ram pump via valve.
9. full-automatic Ames test instrument according to claim 1, is characterized in that, described mixed solution e Foerderanlage comprises mixing inlet pipe, peristaltic pump and blends pipe and vertical lifting device;
Described vertical lifting device is configured to after carry mixed solution in described culture dish, the end blending pipe described in making is moved downward in waste liquid tank, to be delivered in waste liquid tank by the cleaning solution in described tempering tank, thus pipeline is utilized to flow to waste collecting device;
Described mixed solution e Foerderanlage be also configured to utilize described in blend pipe from be in mixed solution add station described culture dish ware dish in aspirate mixed solution, and the ware dish mixed solution of suction told to this culture dish, mix to make the culture dish in the ware dish of this culture dish.
10. full-automatic Ames test instrument according to claim 1, is characterized in that,
The quantity of described cigarette sample test tube is 10, to place 10 kinds of cigarette samples;
The quantity of described multiple microsyringe is 5, supplies liquid, bacterium liquid, solvent, S9 and top layer glue to carry S9 respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520320290.8U CN205011759U (en) | 2015-05-18 | 2015-05-18 | This experiment appearance of full -automatic em |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520320290.8U CN205011759U (en) | 2015-05-18 | 2015-05-18 | This experiment appearance of full -automatic em |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205011759U true CN205011759U (en) | 2016-02-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520320290.8U Expired - Fee Related CN205011759U (en) | 2015-05-18 | 2015-05-18 | This experiment appearance of full -automatic em |
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| Country | Link |
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| CN (1) | CN205011759U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106893672A (en) * | 2017-04-11 | 2017-06-27 | 尹康康 | A kind of soil bacteria stoste takes equipment |
| CN107868748A (en) * | 2016-09-26 | 2018-04-03 | 北京慧荣和科技有限公司 | Culture dish stores sorting equipment and Ames test instrument |
| CN108220162A (en) * | 2018-02-01 | 2018-06-29 | 何加铭 | Virus culture device and its control method |
-
2015
- 2015-05-18 CN CN201520320290.8U patent/CN205011759U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107868748A (en) * | 2016-09-26 | 2018-04-03 | 北京慧荣和科技有限公司 | Culture dish stores sorting equipment and Ames test instrument |
| CN107868748B (en) * | 2016-09-26 | 2023-09-26 | 北京慧荣和科技有限公司 | Ames tester |
| CN106893672A (en) * | 2017-04-11 | 2017-06-27 | 尹康康 | A kind of soil bacteria stoste takes equipment |
| CN108220162A (en) * | 2018-02-01 | 2018-06-29 | 何加铭 | Virus culture device and its control method |
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