CN206089650U - Sampling system and ames test appearance - Google Patents
Sampling system and ames test appearance Download PDFInfo
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- CN206089650U CN206089650U CN201621082979.2U CN201621082979U CN206089650U CN 206089650 U CN206089650 U CN 206089650U CN 201621082979 U CN201621082979 U CN 201621082979U CN 206089650 U CN206089650 U CN 206089650U
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Abstract
The utility model provides a sampling system and ames test appearance. Sampling system includes: a plurality of mixed test tubes, a drive arrangement configures every mixed test tube of at least messenger into and moves first station and second station, a plurality of sample storage test tubes are used for holding multiple sample respectively, sampling device configures into and takes out the sample and carry the sample that takes out to the mixed in vitro that is in first station from a sample storage test tube, a plurality of solution storage container are used for holding multiple solution respectively, and solution remove device, configure into and take out the solution among a plurality of solution storage container according to predetermined order and carry to the mixed in vitro that is in the second station. The utility model discloses a because have a plurality of mixed test tubes and a drive arrangement, the preparation of multiple mixed liquid be can realize simultaneously among the sampling system and ames test appearance, the convenience and the experimental efficiency that have improved the experiment are being shown.
Description
Technical field
The utility model is related to Ames test technical field, more particularly to a kind of sample adding system and with the sample adding system
Ames test instrument.
Background technology
The Jing such as B.N.Ames more than ten years effort, set up and constantly develops perfect salmonella back mutation examination in 1975
Test (also known as Salmonella reversion test, Ames test) widely to be adopted by countries in the world.The method than faster, it is easy, sensitive, economical,
And suitable for test mixing thing, reflect the comprehensive effect of multiple pollutant.Can be with Salmonella reversion test detection food additives, cosmetic
The mutagenicity of product etc., thus speculates its carcinogenicity;With Salmonella reversion test detect Source water and drinking water mutagenicity, explore compared with
The relatively sanitary safe disinfectant measure of current methods;Or the mutagenicity of detection municipal sewage and industrial wastewater, with reference to chemistry point
Analysis, follows the trail of pollution sources, and to study Preventing Countermeasures foundation is provided;Detection soil, sludge, industrial residue compost, the cause of waste ashes
Mutability, after preventing the soil for maintaining life from being polluted by mutagen, by the murrain mankind;Detection gaseous contaminant
Mutagenicity, prevent pollutant via air, there is potential hazard to human body by breathing;Compound is studied with Salmonella reversion test
Structure and the relation of mutagens, theoretical foundation is provided for synthesis to noval chemical compound of the environment without potential hazard;Detection agricultural chemicals is micro-
Mutagenicity before and after biodegradable, understands agricultural chemicals whether there is hidden danger in metabolic process to the mankind after application;Also useful Ames examinations
The anti-mutation-ure of screening is tested, new anticarcinogen etc. is researched and developed.Existing Ames test be all experimenter using dropper to
Addition reagent in culture dish, automaticity is low, and cost of labor is high, although ensure that certain experimental precision, but also can be by
Cause experimental result deviation huge in the error of people.
Utility model content
The purpose of this utility model aims to provide a kind of sample adding system, and it can be automatically by many kinds of substance according to predetermined amount
It is added in mixing test tube and is mixed, automaticity, the cost of labor for significantly improving experiment is low, and experimental precision is high,
To realize that full automatic Ames test instrument provides support.
For this purpose, the utility model provides a kind of sample adding system for Ames test instrument, it includes:
Multiple mixing test tubes;
First driving means, are configured at least make each described mixing test tube move to the first station and the second station, and
First station and second station are in same position, or first station and second station are respectively at two
Individual position;
Multiple samples store test tube, are respectively used to hold various samples;
Sampler, be configured to take out sample from a sample storage test tube and be delivered on the sample of taking-up from
In the mixing test tube of first station;
Multiple solution storage vessels, are respectively used to hold various solution;And
Solution withdrawing device, is configured in a predetermined sequence take out the solution in the plurality of solution storage vessel simultaneously
It is delivered in the mixing test tube of second station.
Further, the plurality of sample stores test tube along a circumferencial direction interval setting;And
The sampler includes:
Sampling needle tubing and the first rotating lifting gear, first rotating lifting gear is configured at least drive the sampling
Needle tubing turns to the top of the mixing test tube in first station or each described sample stores the top of test tube, with
And be configured to drive the sampling needle tubing to carry out elevating movement;
First plunger displacement pump, connects via pipeline with the sampling needle tubing, so that the sampling needle tubing is from a sample
Storage test tube suction sample and the sample entered in the sampling needle tubing is set to spue in described the from the sampling needle tubing
In the mixing test tube of one station.
Further, the solution withdrawing device includes:
Second rotating lifting gear and it is multiple take liquid needle tubing, second rotating lifting gear is configured to drive described in each
Take liquid needle tubing turn to the mixing test tube in second station top or solution storage vessel it is upper
Side, and be configured to drive and take liquid needle tubing and carry out elevating movement described in each;
Second plunger displacement pump, connects via pipeline with liquid needle tubing is taken each Suo Shu;And
First flow path switching device, is configured to controllably turn on liquid needle tubing and second plunger displacement pump is taken described in one, with
Controllably make second plunger displacement pump promote to be taken described in each liquid needle tubing from inhalation solution in a solution storage vessel and
Make into this solution taken in liquid needle tubing take from this to spue in liquid needle tubing in the mixing test tube of second station.
Further, when first station and second station are in same position, in described in the position
Mixing test tube is first received after a kind of sample, then receives various solution, to form mixed liquor;
When first station and second station are respectively at two positions, the first driving means are also configured that
Into making each described mixing test tube first move to first station, after first station receives a kind of sample
Second station is moved to, to receive various solution, so as to form mixed liquor, is then again moved to the mixing test tube described
First station.
Further, the sampler also includes:
Sample presentation needle tubing, is installed on first rotating lifting gear, with the drive of first rotating lifting gear
The top of the mixing test tube in first station is turned to, and under the drive of first rotating lifting gear
Carry out elevating movement;And the sample presentation needle tubing is connected via pipeline with first plunger displacement pump, with from first station
The mixing test tube in absorb the mixed liquor;With
Second flow path switching device, is configured to controllably turn on the sample presentation needle tubing and first plunger displacement pump, or conducting
The sampling needle tubing and first plunger displacement pump.
Further, the sample adding system also includes:
Rinse bath, first rotating lifting gear is configured to drive the sampling needle tubing or the sample presentation needle tubing to rotate
To the top of the rinse bath;
Air compressor, its outlet is controllably turned on the import of the second flow path switching device;
Water pump, its import controllably joining water box or alcohol container, its outlet is controllably filled with second flow path switching
The import put is turned on or turned on the rinse bath.
Further, first station and second station are respectively at two positions;And
The sample adding system is configured to:
The first driving means make multiple adjacent mixing test tubes move to first station successively, described
First plunger displacement pump makes the sample for sucking scheduled volume in the sampling needle tubing from a sample storage test tube, then makes described
Sample in sampling needle tubing spues to the plurality of adjacent mixing test tube;
After the plurality of adjacent mixing test tube receives each sample of corresponding scheduled volume, the first driving means
A mixing test tube for making the plurality of adjacent mixing test tube moves to shifting after the second station reception various solution
First station is moved, first rotating lifting gear makes the sample presentation needle tubing, and back and forth lifting is multiple, first plunger
Pump makes the invisible spectro mixed liquor of the mixing enter the sample presentation needle tubing, after, the first driving means make the plurality of adjacent
Another mixing test tube of mixing test tube carries out above-mentioned action, until the plurality of adjacent mixing test tube completes above-mentioned dynamic
Make;
First plunger displacement pump makes the invisible spectro mixed liquor of the mixing enter after the sample presentation needle tubing, first plunger displacement pump
Make the mixed liquor in the sample presentation needle tubing fifty-fifty enter multiple mixed liquors to receive in container.
Further, the plurality of mixing test tube is along a circumferencial direction interval setting.
Further, the first driving means include:
Rotating cylinder, the plurality of mixing test tube is installed on the rotating cylinder;
Motor and transmission mechanism, the transmission mechanism is configured to for the rotary motion of the motor to be converted into institute
State the rotary motion of rotating cylinder.
The utility model additionally provides a kind of Ames test instrument, and it includes any of the above-described kind of sample adding system.
Because having multiple mixing test tubes and first to drive dress in sample adding system of the present utility model and Ames test instrument
Put, can simultaneously realize the preparation of various mixed liquors, significantly improve the convenience and conventional efficient of experiment.Further, since taking
The special construction of sampling device and solution withdrawing device, can make the simple structure of sample adding system and Ames test instrument, controlling party
Just.
Description of the drawings
Describe some concrete realities of the present utility model in detail by way of example, and not by way of limitation with reference to the accompanying drawings hereinafter
Apply example.Identical reference denotes same or similar part or part in accompanying drawing.It should be appreciated by those skilled in the art that
What these accompanying drawings were not necessarily drawn to scale.In accompanying drawing:
Fig. 1 is the schematic diagram of the sample adding system according to the utility model one embodiment;
Fig. 2 is the schematic partial schematic of the sample adding system according to the utility model one embodiment;
Fig. 3 is the schematic diagram of the Ames test instrument according to the utility model one embodiment;
Fig. 4 is the schematic local structural graph of the Ames test instrument according to the utility model one embodiment;
Fig. 5 is the signal that culture dish stores sorting equipment in Ames test instrument according to the utility model one embodiment
Property structure chart;
Fig. 6 is the schematic local structural graph of the Ames test instrument according to the utility model one embodiment.
Specific embodiment
To make purpose, technical scheme and the advantage of the utility model embodiment clearer, below in conjunction with accompanying drawing to tool
Body embodiment is described in detail.In the utility model embodiment it should be noted that some pipelines in the example shown only by
Portions are shown, some pipelines have been not shown in figure.
Fig. 1 is the schematic diagram of the sample adding system 400 according to the utility model one embodiment, as shown in figure 1, this
Utility model embodiment provides a kind of sample adding system 400 for Ames test instrument.The sample adding system 400 may include multiple
Mixing test tube 410, first driving means, multiple samples storage test tube 420, sampler 430, multiple solution storage vessels 440
With solution withdrawing device 450.
First driving means are configured at least to make each mixing test tube 410 to move to the first station and the second station, and the
One station and the second station are in same position, or the first station and the second station are respectively at two positions.
Multiple sample storage test tubes 420 are respectively used to hold various samples.
Sampler 430 can be configured to from sample storage test tube 420 and take out sample and by the sample conveying taken out
To in the mixing test tube 410 of the first station.
Multiple solution storage vessels 440 are respectively used to hold various solution.
Solution withdrawing device 450 is configured in a predetermined sequence take out the solution in multiple solution storage vessels 440
And be delivered in the mixing test tube 410 of the second station.
In this embodiment, when the first station and the second station are in same position, in the mixing test tube of the position
410 first receive after a kind of sample, then receive various solution, to form mixed liquor.When the first station and the second station are respectively at
During two positions, first driving means are configured to make each mixing test tube 410 first move to the first station, connect in the first station
Receive and the second station is moved to after a kind of sample, to receive various solution, so as to form mixed liquor, then again by the mixing test tube
410 move to the first station.
In the utility model embodiment, using multiple mixing test tubes 410 and first driving means, a mixing examination can be made
Pipe 410 moves to the first station and the second station, the sample in various solution and a sample storage test tube 420 is received, with shape
Into a kind of mixed liquor.Then, another mixing test tube 410 can be made to move to the first station and the second station, receives various samples
Another sample in test tube 420 is stored with another sample, to form another kind of mixed liquor.Multiple mixing test tubes 410 can be formed
Various mixed liquors.
In some embodiments of the present utility model, multiple samples store test tube 420 along a circumferencial direction interval setting.Take
Sampling device 430 may include to sample needle tubing 433, the first rotating lifting gear 431 and the first plunger displacement pump 432.First rotation and lifting is filled
Put 431 to be configured at least drive sampling needle tubing 433 to turn to the top for mixing test tube 410 or each sample in the first station
The top of storage test tube 420, and be configured to drive sampling needle tubing 433 to carry out elevating movement.First plunger displacement pump 432 is via pipeline
Connect with sampling needle tubing 433, so that sampling needle tubing 433 is from a sample storage test tube 420 suction sample and makes to enter sampling probe
Sample in pipe 433 spues in the mixing test tube 410 of the first station from sampling needle tubing 433.
When needing to take out the sample in a sample storage test tube 420, sampling needle tubing 433 can be first set to turn to the sample
The top of storage test tube 420, then makes sampling needle tubing 433 decline the lower section for stretching into solution in the sample storage test tube 420, then
Control the first plunger displacement pump 432 to work, the sample in the sample storage test tube 420 is entered in sampling needle tubing 433.Secondly, rise
Needle tubing 433 is sampled with rotating so as to turn to the top of the mixing test tube 410 in the first station, then make sampling needle tubing 433
Decline, finally make into the sample in sampling needle tubing 433 to be spued to the mixing test tube in the first station from sampling needle tubing 433
In 410, the taking-up of sample is completed.
In some embodiments of the present utility model, as shown in Fig. 2 solution withdrawing device 450 includes the second rotation and lifting
Device 451, multiple take liquid needle tubing 452, the second plunger displacement pump 453 and first flow path switching device.Second rotating lifting gear 451 is matched somebody with somebody
Be set to drive each to take liquid needle tubing 452 turns to the top of mixing test tube 410 in the second station or the storage of solution is held
The top of device 440, and be configured to drive each to take liquid needle tubing 452 and carry out elevating movement.Second plunger displacement pump 453 via pipeline with
Each takes liquid needle tubing 452 and connects.First flow path switching device is configured to controllably to turn on one and takes the plunger of liquid needle tubing 452 and second
Pump 453, it is molten controllably to make the second plunger displacement pump 453 promote each to take the suction from a solution storage vessel 440 of liquid needle tubing 452
Liquid takes in liquid needle tubing 452 from this with the solution for making into this to take in liquid needle tubing 452 and spues to the mixing test tube in the second station
In 410.
When needing to take out various solution, two ways can be passed through:Mode one, can make multiple liquid needle tubings 452 that take in many
The top of individual solution storage vessel 440, then make it is multiple take liquid needle tubing 452 decline stretch in multiple solution storage vessels 440.The
One flow passage selector device first makes one to take liquid needle tubing 452 to turn on the second plunger displacement pump 453, so that this takes suction in liquid needle tubing 452
Solution.Then second flow path switching device makes another take liquid needle tubing 452 to turn on the second plunger displacement pump 453, so that this takes liquid pin
Inhalation solution in pipe 452.Make the 3rd to take liquid needle tubing 452 followed by second flow path switching device to turn on plunger displacement pump, so that should
Take inhalation solution in liquid needle tubing 452.…….Until last takes suction in liquid needle tubing 452 having after corresponding solution.Rise multiple
Second plunger displacement pump 453, rotation is multiple to take liquid needle tubing 452, multiple liquid needle tubings 452 that take is turned to successively in the mixed of the second station
The top of test tube 410 is closed, when certain takes top of the liquid needle tubing 452 in the mixing test tube 410, first flow path switching device is led
Lead to this and take the plunger displacement pump 453 of liquid needle tubing 452 and second, so as to this takes the solution in liquid needle tubing 452 spue in the second station
Mixing test tube 410.Mode two, can make multiple tops for taking liquid needle tubing 452 in multiple solution storage vessels 440, then make many
It is individual take liquid needle tubing 452 decline stretch in multiple solution storage vessels 440.First flow path switching device first makes one to take liquid needle tubing
452 turn on the second plunger displacement pump 453, so that this takes inhalation solution in liquid needle tubing 452.Rise and rotate it is multiple take liquid needle tubing 452,
The top that liquid needle tubing 452 turns to the mixing test tube 410 in the second station that takes of inhalation solution is made, makes this take liquid needle tubing 452
Interior solution spues to the mixing test tube 410 in the second station.Then multiple sampling needle tubings 433 are rotated, makes multiple to take liquid pin
Top of the pipe 452 in multiple solution storage vessels 440, then make it is multiple take liquid needle tubing 452 and decline stretch into the storage of multiple solution
In container 440.First flow path switching device makes another take liquid needle tubing 452 to turn on the second plunger displacement pump 453, so that this takes liquid pin
Inhalation solution in pipe 452.Rise and rotate it is multiple take liquid needle tubing 452, the liquid needle tubing 452 that takes for making inhalation solution is turned in the
The top of the mixing test tube 410 of two stations, makes the solution taken in liquid needle tubing 452 spue to the mixing test tube in the second station
410.…….Until the solution in all of solution storage vessel 440 is all removed and is put into the mixing examination in the second station
In pipe 410.
In some embodiments of the present utility model, sampler 430 may also include sample presentation needle tubing 434 and second flow path is cut
Changing device.Sample presentation needle tubing 434 is installed on the first rotating lifting gear 431, to turn under the drive of the first rotating lifting gear 431
Move to the top of the mixing test tube 410 in the first station, and lifted under the drive of the first rotating lifting gear 431
Motion;And sample presentation needle tubing 434 is connected via pipeline with the first plunger displacement pump 432, with from the mixing test tube 410 of the first station
Absorb mixed liquor.Second flow path switching device is configured to controllably turn on the plunger displacement pump 432 of sample presentation needle tubing 434 and first, or conducting
The sampling plunger displacement pump 432 of needle tubing 433 and first.First plunger displacement pump 432 is configured to make into the mixed liquor in sample presentation needle tubing 434 to tell
Go out to mixed liquor and receive in container, such as in the ware disk of culture dish.
In some preferred embodiments of the present utility model, the first station and the second station are respectively at two positions.
And sample adding system 400 is configured to:
First driving means make multiple adjacent mixing test tubes 410 move to successively to the first station, the first plunger displacement pump 432
Make in sampling needle tubing 433 from the sample of suction scheduled volume in a sample storage test tube 420, then make in sampling needle tubing 433
Sample spues to multiple adjacent mixing test tubes 410.Each corresponding scheduled volume is received in multiple adjacent mixing test tubes 410
Sample after, first driving means make a mixing test tubes 410 of multiple adjacent mixing test tubes 410 move to the second station to connect
Receive and the first station is moved to after various solution, the first rotating lifting gear 431 makes sample presentation needle tubing 434, and back and forth lifting is multiple, so that
Many kinds of substance in the mixing test tube 410 forms more uniform mixed liquor, and the first plunger displacement pump 432 is made in the mixing test tube 410
Mixed liquor enters sample presentation needle tubing 434, after, first driving means make another mixing test tube of multiple adjacent mixing test tubes 410
410 carry out above-mentioned action, until multiple adjacent mixing test tubes 410 complete above-mentioned action.First plunger displacement pump 432 makes the mixing
Mixed liquor in test tube 410 is entered after sample presentation needle tubing 434, and the first plunger displacement pump 432 makes mixed liquor in sample presentation needle tubing 434 fifty-fifty
Receive in container into multiple mixed liquors.
That is, in this embodiment, first driving means are configured to make multiple adjacent mixing test tubes 410 successively
Move to the first station, in the first station same sample received, difference be the sample that receives number it is inconsistent, to be formed
Sample gradient;After move to the second station, to receive various solution.It is aobvious using multiple mixing test tubes 410 and first driving means
Work improves conventional efficient.
In some embodiments of the present utility model, when sample is different, need to be cleaned to sampling needle tubing 433, when
Sample is different, and and/or sample gradient it is different when, need to clean sample presentation needle tubing 434, therefore, in the embodiment
Sample adding system 400 also include rinse bath 461, air compressor 462, water pump 463, water tank and alcohol container.First rotation rises
Falling unit 431 is configured to the top for driving sampling needle tubing 433 or sample presentation needle tubing 434 to turn to rinse bath 461.Air compressor
462 outlet is controllably turned on the import of second flow path switching device.The import of water pump 463 controllably joining water box or alcohol
Container, the outlet of water pump 463 is controllably turned on the import of second flow path switching device or turned on rinse bath 461.For example, when
When sampling needle tubing 433 or sample presentation needle tubing 434 need cleaning, sampling needle tubing 433 or sample presentation needle tubing 434 are turned to into rinse bath 461
Top, after drop in rinse bath 461, water pump 463 can inject water into rinse bath 461 and needle tubing respectively, to clean needle tubing
It is inside and outside.Then, supply-water pump 463 injects alcohol into rinse bath 461 and needle tubing, and finally, air compressor 462 can be to
Injection air in needle tubing, to realize the cleaning to needle tubing.
In some embodiments of the present utility model, multiple mixing test tubes 410 are along a circumferencial direction interval setting.First drives
Dynamic device may include:Rotating cylinder, multiple mixing test tubes 410 are installed on rotating cylinder;Motor, is configured to drive Rotational Cylindrical
Body is rotated.First driving means can also configure at least mixing test tube 410 of the drive in the first station and carry out back and forth
Motion, so that a kind of sample and various solution in the mixing test tube 410 is well mixed.
Fig. 3 is the schematic diagram of the Ames test instrument according to the utility model one embodiment, as shown in figure 3,
And with reference to Fig. 4 to Fig. 6, the utility model embodiment additionally provides a kind of Ames test instrument, it may include pedestal 100, culture
Sample adding system 400 in ware storage sorting equipment 200, folding lid station apparatus 300 and any of the above-described embodiment, can make Ames
Test realizes automation, on the basis of cost of labor is reduced, improves conventional efficient and experimental precision.
Culture dish storage sorting equipment 200 can be configured to the culture dish for being not added with mixed liquor of storage in it one by one
Ground takes out, and after addition mixed liquor in each culture dish, the culture dish is deposited in it.
Folding lid station apparatus 300 can be configured to the movement for storing the drive culture dish of sorting equipment 200 using culture dish to be made
The ware disk of culture dish and ware lid depart from, and the ware disk of culture dish can be transported at each predetermined station.For example, folding lid work
Position device 300 can ware disk ware lid folding station receive culture dish, then by ware disk be transported to mixed liquor addition station, also need by
The ware disk of culture dish is transported and is back to ware disk ware lid folding station etc..
Sample adding system 400 can be configured to various solution and a kind of sample mixed, and the mixed liquor formed after mixing is defeated
Deliver in the ware disk for adding station in mixed liquor.
In some embodiments of the present utility model, the culture dish storage sorting equipment 200 may include base plate 210, culture
Ware frame 220 and elevating mechanism 230.
Base plate 210 can thereon offer through hole 211, the size of through hole 211 a circular slab and to be installed on pedestal 100
May be slightly larger than or more than the diameter of culture dish, to allow culture dish to pass through.
Culture dish frame 220 is rotatably installed on base plate 210.Culture dish frame 220 can have multiple circumferences along base plate 210
The accommodating chamber of direction arrangement, each accommodating chamber vertically extends, and each accommodating chamber is when the top of through hole 211 is turned to
It is coaxial with through hole 211.A pile culture dish can be placed in each accommodating chamber.
Elevating mechanism 230 can have the support load portion 231 for being arranged at the lower section of through hole 211, and elevating mechanism 230 is configured to:
Support load portion 231 is moved upward in through hole 211 so that turn to it is coaxial with through hole 211, accommodate at least
Culture dish in the accommodating chamber of one culture dish is located in support load portion 231, after move down support load portion 231, and make the receiving
The upper surface of in chamber, bottom culture dish moves to upper table concordant with the upper surface of base plate 210 or less than base plate 210
The position in face so that in the accommodating chamber, the culture dish of bottom is sorted out;And/or
The support load portion 231 for carrying one or more culture dishes is moved up, and under making in one or more culture dishes most
The lower surface of the culture dish of side moves to the position of upper surface concordant with the upper surface of base plate 210 or higher than base plate 210, with
It is stored in one or more culture dishes to turn in the accommodating chamber coaxial with through hole 211.
In some embodiments of the utility model embodiment, multiple accommodating chambers include at least one sorting accommodating chamber and
At least one storage accommodating chamber, each sorting accommodating chamber is configured to deposit culture dish to be sorted, each storage accommodating chamber configuration
Into storage culture dish to be stored.
When specifically used, first place one in each sorting accommodating chamber and pile up culture dish, each culture dish has ware lid and ware
Disk.When needing singly to take out the culture dish in a sorting accommodating chamber, first it is moved upward to support load portion 231 logical
In hole 211, make the upper surface in support load portion 231 about concordant with the upper surface of base plate 210.Culture dish frame 220 is rotated, the sorting is made
Accommodating chamber turns to the top of through hole 211, and the presence due to holding in the palm load portion 231, the culture dish in the sorting accommodating chamber is located at support and carries
In portion 231, will not be fallen by through hole 211.Then, with elevating mechanism 230 support load portion 231 can be made slowly to decline.When the sorting is held
Receive in chamber, bottom culture dish upper surface move to it is concordant with the upper surface of base plate 210 or upper less than base plate 210
During position (preferably concordantly, being slightly above or below) on surface, stop declining, rotate culture dish frame 220, to prevent from holding in the palm
When load portion 231 declines again, it is not necessary to which the culture dish of taking-up also falls.Followed by, continue to decline support load portion 231, make taking-up
Culture dish depart from through hole 211 and culture dish frame 220, carry out follow-up action in order to Ames test instrument.
After many kinds of substance is added in the culture dish for taking out, need the culture dish storage.Specifically, the culture dish is made
In the support load portion 231 of elevating mechanism 230, support load portion 231 carries the culture dish and moves up, when the upper surface of the culture dish
The position for moving to upper surface concordant with the upper surface of base plate 210 or less than base plate 210 (preferably concordantly, is slightly above or omits
Less than) when, stop rising, culture dish frame 220 is rotated, so that a storage accommodating chamber is turned in the upper of through hole 211
Side.Then the lower surface for making the culture dish moves to upper surface concordant with the upper surface of base plate 210 or higher than base plate 210
Position.Culture dish frame 220 is finally rotated, after preventing from asking load portion 231 to decline, the culture dish stored in accommodating chamber falls.So
Culture dish can be made to be stored in storage accommodating chamber.
Further, culture dish frame 220 may include multiple hack levers for vertically extending, upper backup pad frame and lower support
Grillage.Multiple hack levers limit multiple accommodating chambers, for example, limit an accommodating chamber per four hack levers.Lower supporting plate frame and upper
Fagging frame, is respectively arranged in the two ends of each hack lever.Elevating mechanism 230 may also include straight line slide unit, to drive in support load portion 231
Lower movement.Support load portion 231 is preferably bracket or pallet.The lower section of base plate 210 is additionally provided with tumbler, is configured to drive culture
Ware frame 220 is rotated.
Preferably, culture dish storage sorting equipment 200 also includes at least one collet 240, is arranged at each storage and accommodates
The bottom in chamber, each collet 240 is configured to be moved in the presence of the culture dish that support load portion 231 carries is moved up, to allow
The culture dish that support load portion 231 carries is moved upward to its top, and after the culture dish that support load portion 231 carries is moved to above it
Initial position is returned to, to hold in the palm the culture dish for carrying thereon.This kind of setting can make the storing step of culture dish simpler, work as taking-up
Culture dish in be added after many kinds of substance, when needing to store in the culture dish, a storage accommodating chamber is rotated everywhere
In the top of through hole 211, the culture dish is then made in the support load portion 231 of elevating mechanism 230, support load portion 231 carries the training
Foster ware is moved up, until the height in support load portion 231 is higher than collet 240, collet 240 first allows to ask load portion and culture dish to pass through,
After return initial position, then ask load portion 231 to decline, culture dish can be located on collet 240, and this kind of structure can be easy to hold in the palm load portion
231 whereabouts.Further, collet can also make have gap between a pile culture dish and base plate 210, be easy to user to take out culture
Ware.Specifically, each collet includes at least three support columns, and one end of each support column is rotatably installed on a storage and holds
Receive the bottom in chamber, be rotated up when being moved up with the culture dish carried in pallet, so as to the culture dish that allows pallet to carry to
On be moved to its top, and after the culture dish carried in pallet is moved to above it to it is lower rotation return to initial position, from
And utilize its bar portion to support culture dish.
In some embodiments of the present utility model, folding lid station apparatus 300 may include support, upper disk 310 and station
Rotating disk 320.Support is fixed on pedestal 100.Upper disk 310 is rotatably installed on support, and with multiple first step holes.Station
Rotating disk 320 is rotatably installed on support and in the lower section of upper disk 310, and has multiple second steps on station turnplate 320
Hole.Preferably, the quantity in first step hole and second step hole is equal.
The elevating mechanism 230 of culture dish storage sorting equipment 200 is configured to:A first step hole with one second
When stepped hole is at coaxial position, the support load portion 231 for carrying culture dish is set to move down from the top of upper disk 310, so that support
Load portion 231 sequentially passes through the first step hole and the second step hole, and during asking load portion 231 to move, makes culture dish
Ware lid 720 is located at the first step hole and makes the ware disk 710 of culture dish be located at the second step hole, so that the ware lid of culture dish
Separate with ware disk;Coaxial position is in the first step hole that ware lid is carried at a second step hole for carrying ware disk
During place, support load portion 231 is set to move up from the lower section of station turnplate 320, so that support load portion 231 sequentially passes through the second step hole
With the first step hole, and during support load portion 231 moves, the ware disk for making culture dish moves up ware lid with culture dish
Coordinate, so that the ware lid of culture dish is installed on ware disk.
That is, after elevating mechanism 230 one by one takes out culture dish, elevating mechanism 230 continues to decline, successively
Through the first step hole in ware disk ware lid folding station and second step hole, ware lid 720 is set to be located at the platform in first step hole
On terrace, ware disk 710 is located on the step surface in second step hole, ware disk is separated with ware lid.Then, 320 turns of station turnplate
It is dynamic, ware disk is transported to into mixed liquor addition station, sample adding system 400 conveys mixed liquor into ware disk 710, then station turnplate
Ware dribbling is returned ware disk ware lid folding station by 320.Now, the support load portion 231 in the lower section of station turnplate 320 carries out ascending motion
Ware lid is set to be covered on ware disk, continuing ascending motion makes culture dish be stored in culture dish storage sorting equipment 200.
In embodiment of the present utility model, station turnplate 320 and upper disk 310 need synchronous axial system, to ensure same training
The ware disk of foster ware and the synchronism of ware lid.In such cases, ware disk ware lid folding station and mixed liquor addition station can same steppings
Action is made, and is separated with ware lid or combination with when mixed liquor is added, can also make another culture dish carry out ware disk.And
And, station turnplate 320 with diameter greater than upper disk 310, to prevent upper disk 310 from blocking the inconvenience for causing to station turnplate 320
Add mixed liquor in ware disk.
In some embodiments of the present utility model, Ames test instrument may also include oscillating uniform device 500, for making ware disk
Interior mixed liquor is further well mixed.Specifically, the oscillating uniform device 500 includes base, driving wheel, driven pulley, shaking-up frame, liter
Falling unit and carrying disk.Floor installation is in pedestal 100.Driving wheel and driven pulley are rotatably installed on base.Shaking-up frame exists
Two position is rotatably installed on respectively driving wheel and driven pulley.
Especially, in this embodiment, base, driving wheel, driven pulley and shaking-up frame collectively form crank and rocker mechanism or
Parallel-crank mechanism.Further, shaking-up frame includes that substrate and upper end are arranged at the side plate on a side of substrate.Substrate
Driving wheel and driven pulley are rotatably installed on respectively at two positions of its lower surface.For example, substrate is in its lower surface
Two positions offer turn hole;Oscillating uniform device 500 also includes two rollers, is respectively arranged in the upper table of driving wheel and driven pulley
Face, and be located at respectively in two turn holes.Lowering or hoisting gear is installed on side plate.Carrying disk is used for culture dish holding, and is installed on lifting
Device, to carry out elevating movement under the drive of lowering or hoisting gear.Carrying disk is preferably sucker.Lowering or hoisting gear can at least make culture dish
Ware disk detachment second step hole, to prevent during rotationally, second step hole hinder ware disk motion.In order to more
Carrying disk is set well and more accurately to go to predetermined position to carry the ware disk of culture dish, oscillating uniform device 500 also includes position detection dress
Put, be configured to detect whether shaking-up frame is in or returns to initial position.For example, position detecting device includes being installed on the side of substrate
Signal patch at edge and the sensor being installed on base.
In some embodiments of the present utility model, Ames test instrument may also include stamp device 600, be configured at place
Mark is set in the wall outer surface or diapire outer surface of the ware disk of stamp station, is caused with preventing that mark is arranged on ware lid
It is chaotic.Specifically, stamp device 600 may include code seat, at least one slide block, at least one stamp pen and cylinder.Code seat is installed
In pedestal 100.At least one slide block is slidably mounted on code seat.Each stamp pen is fixed on a slide block, with cunning
Block carries out stretching motion, so as to arrange mark in the wall outer surface of the ware disk in stamp station.Cylinder arrangement is into controllably
The stamp pen that each slide block is installed on the slide block is set to carry out stretching motion.Further, code seat includes hang plate and sets
The gripper shoe being placed in below hang plate.At least one slide block is installed on hang plate, is carried out with direction of the edge parallel to hang plate
Stretching motion.The nib of each stamp pen obliquely downward, is arranged on the side wall of ware disk with preferably will mark.
The above is only preferred embodiment of the present utility model, it is noted that for the common skill of the art
For art personnel, on the premise of without departing from the utility model principle, any improvements and modifications made also should be regarded as this reality
With new protection domain.
Claims (10)
1. a kind of sample adding system for Ames test instrument, it is characterised in that include:
Multiple mixing test tubes;
First driving means, are configured at least make each described mixing test tube move to the first station and the second station, and described
First station and second station are in same position, or first station and second station are respectively at two positions
Put;
Multiple samples store test tube, are respectively used to hold various samples;
Sampler, is configured to take out sample from a sample storage test tube and the sample of taking-up is delivered in institute
State in the mixing test tube of the first station;
Multiple solution storage vessels, are respectively used to hold various solution;And
Solution withdrawing device, is configured to that the solution in the plurality of solution storage vessel is taken out in a predetermined sequence and conveyed
To in the mixing test tube of second station.
2. sample adding system according to claim 1, it is characterised in that
The plurality of sample stores test tube along a circumferencial direction interval setting;And
The sampler includes:
Sampling needle tubing and the first rotating lifting gear, first rotating lifting gear is configured at least drive the sampling needle tubing
The top or each described sample that turn to the mixing test tube in first station store the top of test tube, Yi Jipei
Being set to the drive sampling needle tubing carries out elevating movement;
First plunger displacement pump, connects via pipeline with the sampling needle tubing, so that the sampling needle tubing is from a sample storage
Test tube sucks sample and makes the sample entered in the sampling needle tubing spue in first work from the sampling needle tubing
In the mixing test tube of position.
3. sample adding system according to claim 1, it is characterised in that the solution withdrawing device includes:
Second rotating lifting gear and it is multiple take liquid needle tubing, second rotating lifting gear is configured to drive and take liquid described in each
Needle tubing turns to the top or the top of a solution storage vessel of the mixing test tube in second station, with
And be configured to drive and take liquid needle tubing and carry out elevating movement described in each;
Second plunger displacement pump, connects via pipeline with liquid needle tubing is taken each Suo Shu;And
First flow path switching device, is configured to controllably turn on liquid needle tubing and second plunger displacement pump is taken described in one, with controllable
Make second plunger displacement pump promote to be taken described in each liquid needle tubing from inhalation solution in a solution storage vessel and make into
Enter the solution taken in liquid needle tubing take from this spue in liquid needle tubing in second station the mixing test tube in.
4. sample adding system according to claim 2, it is characterised in that
When first station and second station are in same position, the mixing test tube in the position is first received
After a kind of sample, then various solution are received, to form mixed liquor;
When first station and second station are respectively at two positions, the first driving means are configured to make
Each described mixing test tube first moves to first station, moves after first station receives a kind of sample
To second station, to receive various solution, so as to form mixed liquor, then again the mixing test tube moved to into described first
Station.
5. sample adding system according to claim 4, it is characterised in that the sampler also includes:
Sample presentation needle tubing, is installed on first rotating lifting gear, to rotate under the drive of first rotating lifting gear
To the top of the mixing test tube in first station, and carry out under the drive of first rotating lifting gear
Elevating movement;And the sample presentation needle tubing is connected via pipeline with first plunger displacement pump, with from the institute in first station
State in mixing test tube and absorb the mixed liquor;With
Second flow path switching device, is configured to controllably turn on the sample presentation needle tubing and first plunger displacement pump, or conducting is described
Sampling needle tubing and first plunger displacement pump.
6. sample adding system according to claim 5, it is characterised in that also include:
Rinse bath, first rotating lifting gear is configured to drive the sampling needle tubing or the sample presentation needle tubing to turn to institute
State the top of rinse bath;
Air compressor, its outlet is controllably turned on the import of the second flow path switching device;
Water pump, its import controllably joining water box or alcohol container, its outlet controllably with the second flow path switching device
Import is turned on or turned on the rinse bath.
7. sample adding system according to claim 5, it is characterised in that
First station and second station are respectively at two positions;And
The sample adding system is configured to:
The first driving means make multiple adjacent mixing test tubes move to first station successively, and described first
Plunger displacement pump makes the sample for sucking scheduled volume in the sampling needle tubing from a sample storage test tube, then makes the sampling
Sample in needle tubing spues to the plurality of adjacent mixing test tube;
After the plurality of adjacent mixing test tube receives each sample of corresponding scheduled volume, the first driving means make institute
A mixing test tubes for stating multiple adjacent mixing test tubes move to second station and receive and move to after various solution
First station, first rotating lifting gear makes the sample presentation needle tubing, and back and forth lifting is multiple, and first plunger displacement pump makes
The invisible spectro mixed liquor of the mixing enters the sample presentation needle tubing, after, the first driving means make the plurality of adjacent mixing
Another mixing test tube of test tube carries out above-mentioned action, until the plurality of adjacent mixing test tube completes above-mentioned action;
First plunger displacement pump makes the invisible spectro mixed liquor of the mixing enter after the sample presentation needle tubing, and first plunger displacement pump makes institute
State the mixed liquor in sample presentation needle tubing fifty-fifty to enter in multiple mixed liquors reception container.
8. sample adding system according to claim 1, it is characterised in that
The plurality of mixing test tube is along a circumferencial direction interval setting.
9. sample adding system according to claim 8, it is characterised in that the first driving means include:
Rotating cylinder, the plurality of mixing test tube is installed on the rotating cylinder;
Motor and transmission mechanism, the transmission mechanism is configured to for the rotary motion of the motor to be converted into described turning
The rotary motion of dynamic cylinder.
10. a kind of Ames test instrument, it is characterised in that including the sample adding system any one of claim 1 to 9.
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CN201621082979.2U CN206089650U (en) | 2016-09-26 | 2016-09-26 | Sampling system and ames test appearance |
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CN201621082979.2U CN206089650U (en) | 2016-09-26 | 2016-09-26 | Sampling system and ames test appearance |
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CN107515194A (en) * | 2017-09-28 | 2017-12-26 | 崔林 | A kind of urine detection instrument and its detection method |
CN109781676A (en) * | 2017-11-10 | 2019-05-21 | 陕西秦云农产品检验检测有限公司 | A kind of aflatoxin biodegrade testing tool and the method for breeding using it |
CN110275039A (en) * | 2018-03-15 | 2019-09-24 | 北京慧荣和科技有限公司 | Sample pipetting volume system and Ames test instrument |
CN110272815A (en) * | 2018-03-15 | 2019-09-24 | 北京慧荣和科技有限公司 | Full-automatic Ames test instrument |
CN110271856A (en) * | 2018-03-15 | 2019-09-24 | 北京慧荣和科技有限公司 | Mixing vessel transfer system and Ames test instrument |
CN110272815B (en) * | 2018-03-15 | 2022-11-22 | 北京慧荣和科技有限公司 | Full-automatic Ames experimental instrument |
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