CN107868749A - Culture dish opening-closing cover device and Ames test instrument - Google Patents
Culture dish opening-closing cover device and Ames test instrument Download PDFInfo
- Publication number
- CN107868749A CN107868749A CN201610853349.9A CN201610853349A CN107868749A CN 107868749 A CN107868749 A CN 107868749A CN 201610853349 A CN201610853349 A CN 201610853349A CN 107868749 A CN107868749 A CN 107868749A
- Authority
- CN
- China
- Prior art keywords
- culture dish
- ware
- disk
- station
- closing cover
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
Abstract
The invention provides a kind of culture dish opening-closing cover device and Ames test instrument.Culture dish opening-closing cover device includes：Support；Upper disk, it regularly or can be rotatably mounted to the support, and have at least one first step hole thereon；Station turnplate, it regularly or can be rotatably mounted to the support and the lower section in the upper disk, and have at least one second step hole on the station turnplate；And elevating mechanism, it has support load portion.The culture dish opening-closing cover device and Ames test instrument of the present invention is because have upper disk, station turnplate and elevating mechanism, the ware lid and ware disk detachment of each culture dish can be made, the ware lid and ware disk that can also make disengaging combine, simple in construction, it is easy to control, especially suitable for full automatic Ames test instrument.Ware disk can be also transported at each station by station turnplate, to be added the operation such as many kinds of substance to ware disk.
Description
Technical field
The present invention relates to Ames test technical field, more particularly to a kind of culture dish opening-closing cover device and there is the culture
The Ames test instrument of ware opening-closing cover device.
Background technology
B.N.Ames etc. was established in 1975 through the effort of more than ten years and is continued to develop perfect salmonella back mutation examination
(also known as Salmonella reversion test, Ames test) is tested widely to have been used by countries in the world.The method than faster, it is easy, sensitive, economical,
And it is applied to test mixing thing, reflect the comprehensive effect of multiple pollutant.Food additives can be detected with Salmonella reversion test, are made up
The mutagenicity of product etc., thus speculate its carcinogenicity；With Salmonella reversion test detection Source water and drinking water mutagenicity, explore compared with
The relatively sanitary safe disinfectant measure of current methods；Or the mutagenicity of detection municipal sewage and industrial wastewater, with reference to chemistry point
Analysis, pollution sources are followed the trail of, foundation is provided for research Preventing Countermeasures；Detect the cause of soil, sludge, industrial residue compost, waste ashes
Mutability, after preventing the soil for maintaining life from being polluted by mutagen, pass through the murrain mankind；Detect gaseous contaminant
Mutagenicity, prevent pollutant via air, potential hazard occurred to human body by breathing；Compound is studied with Salmonella reversion test
The relation of structure and mutagens, theoretical foundation is provided to noval chemical compound of the environment without potential hazard for synthesis；Agricultural chemicals is detected micro-
Mutagenicity before and after biodegradation, understands agricultural chemicals whether there is hidden danger in metabolic process to the mankind after application；Also useful Ames examinations
The anti-mutation-ure of screening is tested, researchs and develops new anticarcinogen etc..Existing Ames test be all experimenter using dropper to
Addition reagent in culture dish, automaticity is low, and cost of labor is high, also can be by although can ensure certain experimental precision
Cause experimental result deviation huge in the error of people.
The content of the invention
The purpose of the present invention aims to provide a kind of culture dish opening-closing cover device, and it can be by the ware disk and ware of each culture dish
Lid separation, after addition many kinds of substance in ware disk, by the ware disk of receiving many kinds of substance and ware lid further in conjunction with full-automatic to realize
Ames test instrument provide support.
Therefore, the invention provides a kind of culture dish opening-closing cover device for Ames test instrument, including：
Support；
Upper disk, it regularly or can be rotatably mounted to the support, and have at least one first step hole thereon；
Station turnplate, it regularly or can be rotatably mounted to the support and the lower section in the upper disk, and described
There is at least one second step hole on station turnplate；With
Elevating mechanism, it has support load portion, and the elevating mechanism is configured to：
When being at coaxial position in a first step hole and a second step hole, make to carry culture dish
The support load portion moved down above the upper disk so that the support load portion successively by the first step hole and this
Two stepped holes, and during support load portion movement, the ware lid of culture dish is located at the first step hole and is made culture dish
Ware disk is located at the second step hole, so that the ware lid of culture dish and the separation of ware disk；With
The first step hole of ware lid and a second step hole for carrying ware disk are carried at one and is in coaxial
During opening position, the support load portion is moved up below the station turnplate so that the support load portion successively by this
Two stepped holes and the first step hole, and during support load portion movement, the ware disk of culture dish is moved up and is trained
The ware lid for supporting ware coordinates, so that the ware lid of culture dish is installed on ware disk.
Further, the station turnplate is rotatably installed on the support, and the station turnplate is configured to position
It is transported in the ware disk in the second step hole at each predetermined station.
Further, the quantity in the first step hole is multiple, and the first step hole and the second step hole
Quantity it is equal；
The station turnplate and the upper disk are rotatably installed on the support, and the station turnplate and the upper disk
Synchronous axial system.
Further, the culture dish opening-closing cover device also includes tumbler, is arranged at the lower section of the station turnplate,
It is configured to drive the station turnplate to rotate.
Further, the culture dish opening-closing cover device also includes link gear, is configured to make the station turnplate and institute
State disk synchronous axial system.
Further, the diameter of the station turnplate is more than the upper disk.
Further, the elevating mechanism also includes straight line slide unit, to drive the support load portion to move up and down.
Further, the support load portion is bracket or pallet.
The present invention also provides a kind of Ames test instrument, and it includes any of the above-described kind of culture dish opening-closing cover device, the training
Support ware opening-closing cover device to be configured to the ware lid of culture dish and the separation of ware disk, after addition mixed liquor in the ware disk, by described in
Ware lid is installed on the ware disk.
Further, the station turnplate of the culture dish opening-closing cover device is configured to the ware disk from ware disk ware lid folding
Station is driven to mixed liquor addition station, and after adding the mixed liquor in the ware disk, by the ware disk from the mixing
Liquid addition station, which drives, arrives the ware disk ware lid folding station.
The culture dish opening-closing cover device and Ames test instrument of the present invention is because have upper disk, station turnplate and lift
Structure, the ware lid and ware disk detachment of each culture dish can be made, the ware lid and ware disk that can also make disengaging combine, simple in construction, controlling party
Just, especially suitable for full automatic Ames test instrument.Ware disk can be also transported at each station by station turnplate, so as to ware
Disk is added the operation such as many kinds of substance.
Brief description of the drawings
Some specific embodiments of the present invention are described in detail by way of example, and not by way of limitation with reference to the accompanying drawings hereinafter.
Identical reference denotes same or similar part or part in accompanying drawing.It should be appreciated by those skilled in the art that these
What accompanying drawing was not necessarily drawn to scale.In accompanying drawing：
Fig. 1 is the schematic diagram according to the culture dish opening-closing cover device of one embodiment of the invention；
Fig. 2 is the schematic diagram according to the Ames test instrument of one embodiment of the invention；
Fig. 3 is the schematic local structural graph according to the Ames test instrument of one embodiment of the invention；
Fig. 4 is the schematic local structural graph according to the Ames test instrument of one embodiment of the invention；
Fig. 5 is the schematic local structural graph according to the Ames test instrument of one embodiment of the invention.
Embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below in conjunction with accompanying drawing to specific real
Example is applied to be described in detail.In embodiments of the present invention it should be noted that some pipelines are only only shown part in the example shown
Pipeline section, some pipelines have been not shown in figure.
Fig. 1 is according to the schematic diagram of the culture dish opening-closing cover device 300 of one embodiment of the invention, such as Fig. 1 institutes
Show, the embodiments of the invention provide a kind of culture dish opening-closing cover device 300 for Ames test instrument.The sky culture dish folding
Lid arrangement 300 may include support, upper disk 310, station turnplate 320 and elevating mechanism 330.
Upper disk 310 regularly or can be rotatably mounted to support, and have at least one first step hole 311.
Station turnplate 320 regularly or can be rotatably mounted to support and the lower section in upper disk 310, and station turnplate
There is at least one second step hole 321 on 320.
Elevating mechanism 330 can have support load portion 331, and elevating mechanism 330 is configured to：
When being at coaxial position in a first step hole 311 and a second step hole 321, make to carry culture dish
Support load portion 331 moves down from the top of upper disk 310 so that support load portion 331 successively by the first step hole 311 and this second
Stepped hole 321, and during the movement of support load portion 331, the ware lid 720 of culture dish is located at the first step hole 311 and is made training
The ware disk 710 for supporting ware is located at the second step hole 321, so that the ware lid 720 and ware disk 710 of culture dish separate；With
It is in the first step for carrying ware lid 720 hole 311 and the second step for carrying ware disk 710 hole 321
When at coaxial position, support load portion 331 is moved up from the lower section of station turnplate 320 so that support load portion 331 successively by this
Two stepped holes 321 and the first step hole 311, and during the movement of support load portion 331, move up the ware disk of culture dish
Coordinate with the ware lid of culture dish, so that the ware lid of culture dish is installed on ware disk.
Specifically, in ware disk and ware the lid separation for needing to make culture dish, a first step hole 311 and one the is first made
Two stepped holes 321 are in coaxial position, and in the top in support load portion 331.Then support load portion 331 is made to rise to disk 310
Top, manually a culture dish is placed in support load portion 331 from other devices or people.Then, elevating mechanism 330 drives culture
Ware declines, and ware lid is located on the step surface in first step hole 311, ware disk is located on the step surface in second step hole 321.Together
Sample, when needing to combine the ware disk positioned at second step hole 321 and the ware disk positioned at first step hole 311, make elevating mechanism
330 drive support load portion 331 to carry out ascending motion.Culture dish opening-closing cover device 300 in the present embodiment can rapidly make
The ware disk of culture dish separates with ware lid, or ware lid is covered on ware disk.
In some embodiments of the invention, first step hole 311 should be equal with the quantity in second step hole 321.When upper
Disk 310 only has a first step hole 311, and when station turnplate 320 only has a second step hole 321, upper disk 310 is securable to
Support, station turnplate 320 are securable to support, are also rotatably mounted in support.The reason for so setting is, works as First
When rank hole 311 and second step hole 321 are one, ware disk ware lid folding station and mixed liquor addition station can be in same positions
Put, also can be in different positions.When in same station, station turnplate 320 can be fixed on support, when not in a station
When, station turnplate 320 can be arranged to be rotatably installed on support.Alternatively, upper disk 310 is also rotatably mounted in
Support, and with the synchronous axial system of station turnplate 320, to ensure in ware disk ware lid folding station, first step hole 311 and second step
Hole 321 is coaxial.
In other embodiments of the present invention, the quantity in first step hole 311 is multiple, and first step hole 311
It is equal with the quantity in second step hole 321.Station turnplate 320 and upper disk 310 are rotatably installed on support, and station turnplate
320 and the synchronous axial system of upper disk 310, to ensure the synchronism of the ware disk of same culture dish and ware lid.In such cases, ware disk ware
Lid folding station and mixed liquor addition station can be at different stations, when being added material, can also make another training
Support separation or combination that ware carries out ware disk and ware lid.
Preferably, in both examples above, when station turnplate 320 is rotatably installed on support, station turnplate
320 are configured to the ware disk in second step hole 321 being transported at each predetermined station.
In some embodiments of the invention, station turnplate 320 and upper disk 310 are rotatably installed on support, and station
When rotating disk 320 and upper 310 synchronous axial system of disk, the culture dish opening-closing cover device 300 in the embodiment also includes tumbler and connection
Motivation structure.Tumbler is arranged at the lower section of station turnplate 320, is configured to drive station turnplate 320 to rotate.Link gear configures
Into making station turnplate 320 and the synchronous axial system of upper disk 310.
Preferably, the diameter of station turnplate 320 is more than upper disk 310, to prevent upper the blocking to station turnplate 320 of disk 310
Caused by inconvenience added in ware disk many kinds of substance formation mixed liquor.The rotary shaft of station turnplate 320 and the rotation of upper disk 310
Shaft parallel is arranged at intervals, and when ensureing at ware disk ware lid folding station, a first step hole 311 and one second
Rank hole 321 is coaxially disposed.
In some embodiments of the invention, elevating mechanism 330 also includes straight line slide unit, to drive support load portion about 331
It is mobile.It is preferably bracket or pallet to hold in the palm load portion 331.
Fig. 2 is according to the schematic diagram of the Ames test instrument of one embodiment of the invention, as shown in Fig. 2 and joining
Fig. 3 to Fig. 5 is examined, the embodiment of the present invention additionally provides a kind of Ames test instrument, and it may include pedestal 100, culture dish storage point
The culture dish opening-closing cover device 300 and sample adding system 400 in device 200, any of the above-described embodiment are picked, Ames test can be made real
Show automation, on the basis of cost of labor is reduced, improve conventional efficient and experimental precision.
Culture dish storage sorting equipment 200 is configured with the elevating mechanism 330 of culture dish opening-closing cover device 300 from culture
The culture dish for being not added with many kinds of substance of storage singly takes out in ware storage sorting equipment 200, treats in each culture dish
After the mixed liquor for adding many kinds of substance composition, by culture dish storage culture dish storage sorting equipment 200.
The culture dish opening-closing cover device 300 is configured to separate on the ware lid 720 of culture dish and ware disk 710, treats the ware
In disk after addition mixed liquor, the ware lid is installed on the ware disk.
Sample adding system 400 can be configured to a variety of solution and a kind of sample mixed, and the mixed liquor formed after mixing is defeated
Deliver in the ware disk on culture dish opening-closing cover device 300.
In some embodiments of the invention, culture dish storage sorting equipment 200 may include bottom plate 210 and culture dish frame
220。
Bottom plate 210 can be a circular slab and be installed on pedestal 100, offer through hole 211, the big I of through hole 211 thereon
It is slightly larger than or the diameter more than culture dish, to allow culture dish to pass through.
Culture dish frame 220 is rotatably installed on bottom plate 210.Culture dish frame 220 can have multiple circumferences along bottom plate 210
The accommodating chamber of direction arrangement, each accommodating chamber vertically extend, and each accommodating chamber is when turning to the top of through hole 211
It is coaxial with through hole 211.A pile culture dish can be placed in each accommodating chamber.
The elevating mechanism 330 of culture dish opening-closing cover device 300 can be configured to：
Support load portion 331 is moved upward in through hole 211 so that turn to through hole 211 it is coaxial, accommodate at least
Culture dish in the accommodating chamber of one culture dish in support load portion 331, after move down support load portion 331, and make the receiving
The upper surface of intracavitary, bottom culture dish is moved to upper table concordant with the upper surface of bottom plate 210 or less than bottom plate 210
The position in face so that in the accommodating chamber, the culture dish of bottom is sorted out；And/or
The support load portion 331 for carrying one or more culture dishes is moved up, and is made most lower in one or more culture dishes
The lower surface of the culture dish of side is moved to the position of upper surface concordant with the upper surface of bottom plate 210 or higher than bottom plate 210, with
One or more culture dishes are stored in turn in the accommodating chamber coaxial with through hole 211.
In some embodiments of the embodiment of the present invention, multiple accommodating chambers are including at least one sorting accommodating chamber and at least
One storage accommodating chamber, each accommodating chamber that sorts are configured to deposit culture dish to be sorted, and each accommodating chamber that stores is configured to deposit
Put culture dish to be stored.
When specifically used, a pile culture dish is first placed in each sorting accommodating chamber, each culture dish has ware lid and ware
Disk.When needing singly to take out the culture dish in a sorting accommodating chamber, first it is moved upward to support load portion 331 logical
In hole 211, make the upper surface in support load portion 331 about concordant with the upper surface of bottom plate 210.Culture dish frame 220 is rotated, makes the sorting
Accommodating chamber turns to the top of through hole 211, and due to the presence in support load portion 331, the culture dish in the sorting accommodating chamber carries positioned at support
In portion 331, it will not be fallen by through hole 211.Then, support load portion 331 can be made slowly to decline with elevating mechanism 330, when the sorting is held
Receive intracavitary, bottom culture dish upper surface be moved to it is concordant with the upper surface of bottom plate 210 or upper less than bottom plate 210
During position (be preferably concordant, be slightly above or below) on surface, stop declining, culture dish frame 220 is rotated, to prevent from holding in the palm
When load portion 331 declines again, it is not necessary to which the culture dish of taking-up also falls.Followed by continuing to decline support load portion 331, make taking-up
Culture dish depart from through hole 211 and culture dish frame 220, carry out ware disk 710 and ware lid to enter culture dish opening-closing cover device 300
720 separation, and add mixed liquor into ware disk using sample adding system 400.
After the mixed liquor of many kinds of substance composition is added in the culture dish of taking-up, culture dish opening-closing cover device 300 makes ware
Disk 710 is combined with ware lid 720, and with reference to rear, support load portion 331 carries the culture dish and continued to move up, when the upper table of the culture dish
Face be moved to upper surface concordant with the upper surface of bottom plate 210 or less than bottom plate 210 position (be preferably it is concordant, slightly above or
When slightly below), stop rising, rotate culture dish frame 220, so that a storage accommodating chamber is turned in through hole 211
Top.Then the lower surface of the culture dish is made to be moved to upper surface concordant with the upper surface of bottom plate 210 or higher than bottom plate 210
Position.Culture dish frame 220 is finally rotated, to prevent after asking load portion 331 to decline, the culture dish stored in accommodating chamber falls.This
Sample can be such that culture dish is stored in storage accommodating chamber.
Further, culture dish frame 220 may include multiple hack levers vertically extended, upper backup pad frame and lower support
Grillage.Multiple hack levers limit multiple accommodating chambers, such as every four hack levers limit an accommodating chamber.Lower supporting plate frame and upper branch
Fagging frame is respectively arranged in the both ends of each hack lever.
Preferably, culture dish storage sorting equipment 200 also includes at least one collet 240, is arranged at each storage and accommodates
The bottom of chamber, the culture dish that each collet 240 is configured to carry in pallet moves in the presence of moving up, to allow pallet to take
The culture dish of load be moved upward to its top, and the culture dish carried in pallet be moved to above it after return to initial position,
With the culture dish of support load thereon.Such a setting can make the storing step of culture dish simpler, be added when in the culture dish of taking-up
Add after many kinds of substance, it is necessary to when the culture dish is stored, a storage accommodating chamber is turned in the upper of through hole 211
Side, then makes the culture dish be in the support load portion 331 of elevating mechanism 330, and support load portion 331 carries the culture dish and moved up,
Until the height in support load portion 331 is higher than collet 240, collet 240 first allows to hold in the palm load portion 331 and culture dish by the way that rear return is initial
Position, then load portion 331 is ask to decline, culture dish can be located on collet 240, and such a structure can be easy to hold in the palm the whereabouts in load portion 231.Enter
One step, collet 240 can also make have gap between a pile culture dish and bottom plate 210, be easy to user to take out culture dish.Specifically
Ground, each collet 240 include at least three support columns, and one end of each support column is rotatably installed on a storage accommodating chamber
Bottom, be rotated up when being moved up with the culture dish carried in pallet, so as to allow the culture dish that pallet carries to moving up
Move to its top, and the culture dish carried in pallet and return to initial position to lower rotation after being moved to above it, so as to profit
Culture dish is supported with its bar portion.
In some embodiments of the invention, the station turnplate 320 of the culture dish opening-closing cover device 300 be configured to by
The ware disk drives to mixed liquor from ware disk ware lid folding station and adds station, and adds the mixed liquor in the ware disk
Afterwards, the ware disk is driven to the ware disk ware lid folding station from mixed liquor addition station.In such cases, station turns
Disk 320 is preferably rotatably installed on support, and the second step hole 321 on station turnplate 320 is preferably several, and upper disk 310 is excellent
Elect as with the synchronous axial system of station turnplate 320, the first step hole 311 on upper disk 310 is preferably also multiple.
In some embodiments of the invention, sample adding system 400 may include it is multiple mixing test tubes 410, first driving means,
Multiple sample storage test tube 420, sampler, multiple solution storage vessels 440 and solution withdrawing devices.
First driving means are configured at least to make each mixing test tube 410 to be moved to the first station and second station, and the
One station and second station are respectively at two positions.
Multiple sample storage test tubes 420 are respectively used to hold a variety of samples.
Sampler can be configured to stored from sample and take out sample in test tube 420 and be delivered to the sample of taking-up from
In in the mixing test tube 410 of the first station.Sampler may be additionally configured to take out mixed liquor out of a mixing test tube 410, so
The mixed liquor of taking-up is transported to afterwards in the ware disk for adding station in mixed liquor.
Multiple solution storage vessels 440 are respectively used to hold a variety of solution.
Solution withdrawing device is configured in a predetermined sequence take out the solution in multiple solution storage vessels 440 and defeated
Deliver in the mixing test tube 410 in second station.
In this embodiment, first driving means are configured to make each mixing test tube 410 first be moved to the first station,
First station is moved to second station after receiving a kind of sample, to receive a variety of solution.Using multiple mixing test tubes 410 and first
Drive device, a mixing test tube 410 can be made to be moved to the first station and second station, a variety of solution are received and a sample is deposited
The sample in test tube 420 is stored up, to form a kind of mixed liquor.Then, can make another mixing test tube 410 be moved to the first station and
Second station, another sample in a variety of samples and another sample storage test tube 420 is received, to form another mixed liquor.
Multiple mixing test tubes 410 can form a variety of mixed liquors.In this embodiment, first driving means are configured to make multiple adjacent
Mixing test tube 410 is moved to the first station successively, receives same sample in the first station, difference is the more of the sample received
It is few inconsistent, to form sample gradient；After be moved to second station, to receive a variety of solution.Using multiple mixing Hes of test tube 410
First driving means significantly improve conventional efficient.
In some embodiments of the invention, multiple samples store test tube 420 and are arranged at intervals along a circumferencial direction.Sampling dress
Putting may include to sample needle tubing, the first rotating lifting gear 431 and the first plunger pump 432.First rotating lifting gear 431 is configured to
At least drive sampling needle tubing turns to the top of the mixing test tube 410 in the first station or each sample stores test tube 420
Top, and be configured to drive sampling needle tubing to carry out elevating movement.First plunger pump 432 connects via pipeline with sampling needle tubing,
So that sampling needle tubing sucks sample from a sample storage test tube 420 and makes to enter the sample in sampling needle tubing out of sampling needle tubing
Spue to the mixing test tube 410 in the first station.
When needing to take out the sample in a sample storage test tube 420, it sampling needle tubing is turned to the sample and deposit
The top of test tube 420 is stored up, sampling needle tubing is declined the lower section for stretching into solution in sample storage test tube 420, then controls
First plunger pump 432 works, and the sample in sample storage test tube 420 is entered in sampling needle tubing.Secondly, rise and rotation takes
Sample needle tubing, make its turn in the first station mixing test tube 410 top, then make sampling needle tubing decline, finally make into
Enter the sample sampled in needle tubing to spue in the mixing test tube 410 of the first station out of sampling needle tubing, complete taking for sample
Go out.
In some embodiments of the invention, solution withdrawing device includes the second rotating lifting gear 451, multiple takes liquid pin
Pipe 452, the second plunger pump 453 and first flow path switching device.Second rotating lifting gear 451, which is configured to drive, each takes liquid pin
Pipe 452 turns to the top of mixing test tube 410 or the top of a solution storage vessel 440 in second station, Yi Jipei
Being set to drive each takes liquid needle tubing 452 to carry out elevating movement.Second plunger pump 453 is via pipeline with each taking liquid needle tubing 452 to connect
It is logical.First flow path switching device, which is configured to controllably to turn on one, takes the plunger pump 453 of liquid needle tubing 452 and second, controllably to make
Second plunger pump 453 promotes each to take liquid needle tubing 452 inhalation solution and to make entrance out of a solution storage vessel 440 this takes liquid
Solution in needle tubing 452 is taken in liquid needle tubing 452 from this to spue to the mixing test tube 410 in second station.
When needing to take out a variety of solution, two ways can be passed through：Mode one, can make it is multiple take liquid needle tubing 452 be in it is more
The top of individual solution storage vessel 440, then make it is multiple take liquid needle tubing 452 decline stretch into multiple solution storage vessels 440.The
One flow passage selector device first makes one to take liquid needle tubing 452 to be turned on the second plunger pump, so that this takes suction in liquid needle tubing 452 molten
Liquid.Then second flow path switching device makes another take liquid needle tubing 452 to be turned on the second plunger pump, so that this takes liquid needle tubing 452
Interior inhalation solution.The 3rd is set to take liquid needle tubing 452 to be turned on plunger pump followed by second flow path switching device, so that this takes liquid pin
Inhalation solution in pipe 452.…….Until after last takes the interior suction of liquid needle tubing 452 to have corresponding solution.Rise and rotate multiple
Liquid needle tubing 452 is taken, makes multiple tops for taking liquid needle tubing 452 to turn to the mixing test tube 410 in second station successively, at some
When taking the liquid needle tubing 452 to be in the top of the mixing test tube 410, first flow path switching device turns on this and takes liquid needle tubing 452 and second
Plunger pump 453, so that this takes the solution in liquid needle tubing 452 to spue to the mixing test tube 410 in second station.Mode two, can
Make multiple tops for taking liquid needle tubing 452 to be in multiple solution storage vessels 440, then make it is multiple take liquid needle tubing 452 decline stretch into
In multiple solution storage vessels 440.First flow path switching device first makes one to take liquid needle tubing 452 to be turned on the second plunger pump, with
This is set to take inhalation solution in liquid needle tubing 452.Rise and rotate it is multiple take liquid needle tubing 452, make inhalation solution takes 452 turns of liquid needle tubing
Move to the top of the mixing test tube 410 in second station, make this take the solution in liquid needle tubing 452 to spue in second station
Mixing test tube 410.Then multiple sampling needle tubings are rotated, make multiple to take liquid needle tubing 452 to be in multiple solution storage vessels 440
Top, then make it is multiple take liquid needle tubing 452 decline stretch into multiple solution storage vessels 440.First flow path switching device makes separately
One takes liquid needle tubing 452 to be turned on the second plunger pump, so that this takes inhalation solution in liquid needle tubing 452.Rise and rotate multiple take
Liquid needle tubing 452, make inhalation solution takes liquid needle tubing 452 to turn to the top of the mixing test tube 410 in second station, takes this
Solution in liquid needle tubing 452 spues to the mixing test tube 410 in second station.…….Until all solution storage vessels
Solution in 440 is all removed and is put into the mixing test tube 410 in second station.
In some embodiments of the invention, sampler may also include sample presentation needle tubing and second flow path switching device.Send
Sample needle tubing is installed on the first rotating lifting gear 431, to be turned under the drive of the first rotating lifting gear 431 in first
The top of the mixing test tube 410 of station, and carry out elevating movement under the drive of the first rotating lifting gear 431；And sample presentation
Needle tubing connects via pipeline with the first plunger pump 432, to absorb mixed liquor out of mixing test tube 410 in the first station.The
Two flow passage selector devices are configured to controllably turn on sample presentation needle tubing and the first plunger pump 432, or conducting sampling needle tubing and the first post
Fill in pump 432.The mixed liquor that first plunger pump is configured to make to enter in sample presentation needle tubing spues to the ware disk of culture dish.
After each mixing test tube 410 receives a kind of sample in the first station, and second station is moved to, and received a variety of
The first station is immediately returned to after solution, sample presentation needle tubing can carry out reciprocal elevating movement, so that many kinds of substance in mixing test tube 410
More uniform mixed liquor is formed, then the invisible spectro mixed liquor of the mixing is delivered in the ware disk of one or more culture dishes.
In some embodiments of the invention, sample adding system 400 also includes rinse bath.The import of second flow path switching device
Air compressor and supply-water pump are controllably also connected by valving.Supply-water pump is additionally operable to provide cleaning fluid, example to rinse bath
Such as, the import of supply-water pump controllably joining water box or alcohol container.First rotating lifting gear 431 is configured to drive sampling needle tubing
The top of rinse bath is turned to sample presentation needle tubing, after drop in rinse bath.When needing cleaning, the first rotating lifting gear
431 can make sampling needle tubing and sample presentation needle tubing is in the top of rinse bath, and supply-water pump first can inject water into rinse bath and needle tubing, with
Clean the internal or external of needle tubing.Then, supply-water pump injects alcohol into rinse bath and needle tubing, and finally, air compressor can be to
Injection air in needle tubing, to realize the cleaning to needle tubing.
In some embodiments of the invention, multiple mixing test tubes 410 are arranged at intervals along a circumferencial direction.First driving dress
Putting may include：Rotating cylinder, multiple mixing test tubes 410 are installed on rotating cylinder；Motor, motor are configured to band turn
Dynamic cylinder is rotated.
In some embodiments of the invention, Ames test instrument may also include oscillating uniform device 500, for making in ware disk
Mixed liquor is further well mixed.Specifically, the oscillating uniform device 500 includes base, driving wheel, driven pulley, shaking-up frame, lifting dress
Put and carrying disk.Floor installation is in pedestal 100.Driving wheel and driven pulley are rotatably installed on base.Shaking-up frame its two
Individual position is rotatably installed on driving wheel and driven pulley respectively.
Especially, in this embodiment, base, driving wheel, driven pulley and shaking-up frame collectively form crank and rocker mechanism or
Parallel-crank mechanism.Further, shaking-up frame is arranged at the side plate on a side of substrate including substrate and upper end.Substrate
Driving wheel and driven pulley are rotatably installed on respectively at two positions of its lower surface.For example, substrate is in its lower surface
Two positions offer turn hole；Oscillating uniform device 500 also includes two rollers, is respectively arranged in the upper table of driving wheel and driven pulley
Face, and respectively in two turn holes.Lowering or hoisting gear is installed on side plate.Carrying disk is used for culture dish holding, and is installed on lifting
Device, to carry out elevating movement under the drive of lowering or hoisting gear.Carrying disk is preferably sucker.Lowering or hoisting gear can at least make culture dish
Ware disk 710 depart from second step hole 321, to prevent during rotationally, second step hole 321 hinders the motion of ware disk.
In order to ware disk that is more preferable and more accurately making carrying disk go to predetermined position to carry culture dish, oscillating uniform device 500 also includes
Position detecting device, it is configured to detect whether shaking-up frame is in or returns to initial position.For example, position detecting device includes installation
Signal patch and the sensor that is installed on base in the edge of substrate.
In some embodiments of the invention, Ames test instrument may also include stamp device 600, be configured in beating
The wall outer surface of the ware disk of code station or bottom wall outer surface set mark, cause to mix to prevent from mark being arranged at ware covering
Disorderly.Specifically, stamp device 600 may include code seat, at least one sliding block, at least one stamp pen and cylinder.Code seat is installed on
Pedestal 100.At least one sliding block is slidably mounted on code seat.Each stamp pen is fixed on a sliding block, with sliding block
Stretching motion is carried out, so as to set mark in the wall outer surface of the ware disk in stamp station.Cylinder arrangement is into controllably making
The stamp pen that each sliding block is installed on the sliding block carries out stretching motion.Further, code seat includes hang plate and setting
Supporting plate below hang plate.At least one sliding block is installed on hang plate, to be stretched along the direction parallel to hang plate
Contracting motion.The nib of each stamp pen obliquely downward, is arranged in the side wall of ware disk with will preferably mark.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, any improvements and modifications made also should be regarded as the protection of the present invention
Scope.
Claims (10)
- A kind of 1. culture dish opening-closing cover device for Ames test instrument, it is characterised in that including：Support；Upper disk, it regularly or can be rotatably mounted to the support, and have at least one first step hole thereon；Station turnplate, it regularly or can be rotatably mounted to the support and the lower section in the upper disk, and the station There is at least one second step hole on rotating disk；WithElevating mechanism, it has support load portion, and the elevating mechanism is configured to：When being in a first step hole and a second step hole at coaxial position, make the institute for carrying culture dish State support load portion to move down above the upper disk, so that the support load portion is successively by the first step hole and this second Rank hole, and during support load portion movement, the ware lid of culture dish is located at the first step hole and is made the ware disk of culture dish Positioned at the second step hole, so that the ware lid of culture dish and the separation of ware disk；WithThe first step hole of ware lid and a second step hole for carrying ware disk are carried at one and is in coaxial position During place, the support load portion is set to be moved up below the station turnplate, so that the support load portion passes through this second successively Rank hole and the first step hole, and during support load portion movement, the ware disk of culture dish is set to move up and culture dish Ware lid coordinate so that the ware lid of culture dish is installed on ware disk.
- 2. culture dish opening-closing cover device according to claim 1, it is characterised in thatThe station turnplate is rotatably installed on the support, and the station turnplate is configured to positioned at the second step Ware disk in hole is transported at each predetermined station.
- 3. culture dish opening-closing cover device according to claim 1, it is characterised in thatThe quantity in the first step hole is multiple, and the first step hole is equal with the quantity in the second step hole；The station turnplate and the upper disk are rotatably installed on the support, and the station turnplate and the upper disk are synchronous Rotate.
- 4. culture dish opening-closing cover device according to claim 3, it is characterised in that also include：Tumbler, the lower section of the station turnplate is arranged at, is configured to drive the station turnplate to rotate.
- 5. culture dish opening-closing cover device according to claim 3, it is characterised in that also include：Link gear, it is configured to make the station turnplate and the upper disk synchronous axial system.
- 6. culture dish opening-closing cover device according to claim 1, it is characterised in thatThe diameter of the station turnplate is more than the upper disk.
- 7. culture dish opening-closing cover device according to claim 1, it is characterised in thatThe elevating mechanism also includes straight line slide unit, to drive the support load portion to move up and down.
- 8. culture dish opening-closing cover device according to claim 1, it is characterised in thatThe support load portion is bracket or pallet.
- A kind of 9. Ames test instrument, it is characterised in that comprising the culture dish opening-closing cover device any one of right 1 to 8, The culture dish opening-closing cover device is configured to the ware lid of culture dish and the separation of ware disk, after addition mixed liquor in the ware disk, The ware lid is installed on the ware disk.
- 10. Ames test instrument according to claim 9, it is characterised in that the station of the culture dish opening-closing cover device Rotating disk, which is configured to from ware disk ware lid folding station drive the ware disk to mixed liquor, adds station, and is added in the ware disk After the mixed liquor, the ware disk is driven to the ware disk ware lid folding station from mixed liquor addition station.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610853349.9A CN107868749B (en) | 2016-09-26 | 2016-09-26 | Culture dish device of uncapping and ames tester |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610853349.9A CN107868749B (en) | 2016-09-26 | 2016-09-26 | Culture dish device of uncapping and ames tester |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107868749A true CN107868749A (en) | 2018-04-03 |
| CN107868749B CN107868749B (en) | 2022-11-22 |
Family
ID=61752090
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610853349.9A Active CN107868749B (en) | 2016-09-26 | 2016-09-26 | Culture dish device of uncapping and ames tester |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107868749B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109470836A (en) * | 2018-10-23 | 2019-03-15 | 中国科学院合肥物质科学研究院 | A kind of Soil K+adsorption alkali-hydrolyzable nitrogen batch detection device |
| CN110240098A (en) * | 2019-05-22 | 2019-09-17 | 广西曼森生物科技有限公司 | Culture medium magazine hood-opening device |
| CN112662544A (en) * | 2021-01-08 | 2021-04-16 | 湖南隋侯珠科技有限公司 | Culture dish uncaps and transfer device, microorganism sample processing system |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0385902A1 (en) * | 1989-03-03 | 1990-09-05 | Armor Equipement Scientifique | Device for filling Petri dishes |
| CN102978103A (en) * | 2012-03-06 | 2013-03-20 | 牛刚 | Manufacturing apparatus and manufacturing method for small sterile prefabricated culture dish |
| CN105460247A (en) * | 2015-05-25 | 2016-04-06 | 郝玉有 | Device for subpackaging culture mediums |
| CN105692199A (en) * | 2016-04-07 | 2016-06-22 | 苏州普洛泰科精密工业有限公司 | Single body feeding and discharging machine for manufacturing LCD products |
| CN206089651U (en) * | 2016-09-26 | 2017-04-12 | 北京慧荣和科技有限公司 | Culture dish opens and shuts and covers device and ames test appearance |
-
2016
- 2016-09-26 CN CN201610853349.9A patent/CN107868749B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0385902A1 (en) * | 1989-03-03 | 1990-09-05 | Armor Equipement Scientifique | Device for filling Petri dishes |
| CN102978103A (en) * | 2012-03-06 | 2013-03-20 | 牛刚 | Manufacturing apparatus and manufacturing method for small sterile prefabricated culture dish |
| CN105460247A (en) * | 2015-05-25 | 2016-04-06 | 郝玉有 | Device for subpackaging culture mediums |
| CN105692199A (en) * | 2016-04-07 | 2016-06-22 | 苏州普洛泰科精密工业有限公司 | Single body feeding and discharging machine for manufacturing LCD products |
| CN206089651U (en) * | 2016-09-26 | 2017-04-12 | 北京慧荣和科技有限公司 | Culture dish opens and shuts and covers device and ames test appearance |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109470836A (en) * | 2018-10-23 | 2019-03-15 | 中国科学院合肥物质科学研究院 | A kind of Soil K+adsorption alkali-hydrolyzable nitrogen batch detection device |
| CN110240098A (en) * | 2019-05-22 | 2019-09-17 | 广西曼森生物科技有限公司 | Culture medium magazine hood-opening device |
| CN112662544A (en) * | 2021-01-08 | 2021-04-16 | 湖南隋侯珠科技有限公司 | Culture dish uncaps and transfer device, microorganism sample processing system |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107868749B (en) | 2022-11-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN206089650U (en) | Sampling system and ames test appearance | |
| CN206089651U (en) | Culture dish opens and shuts and covers device and ames test appearance | |
| CN208279624U (en) | Full-automatic Ames test instrument | |
| CN206096163U (en) | Ames test appearance | |
| US5578495A (en) | Apparatus, and process, for automatically sampling solids and semi-solids materials for analysis | |
| CN107868749A (en) | Culture dish opening-closing cover device and Ames test instrument | |
| CN206089652U (en) | Culture dish storage sorting device and ames test appearance | |
| CN110272815A (en) | Full-automatic Ames test instrument | |
| CN205236005U (en) | This experiment appearance of device, conveyor system and full -automatic em is piled up in culture dish letter sorting | |
| CN1152354A (en) | Modular vial autosampler | |
| CN206408230U (en) | Oscillating uniform device and Ames test instrument | |
| CN106281992A (en) | Full-automatic Ames test instrument | |
| CN208266156U (en) | Test tube mechanism for sorting and Ames test instrument with it | |
| CN107868748A (en) | Culture dish stores sorting equipment and Ames test instrument | |
| CN113916993A (en) | Solid phase extraction instrument and use method thereof | |
| JPH04248980A (en) | Device of automatically testing microorganism | |
| CN101376070B (en) | Hollow fiber membrane type liquid phase micro-extraction apparatus and method of use thereof | |
| CN106018855B (en) | A kind of PCT chromatography intelligent detection devices and its detection method | |
| CN108061786A (en) | A kind of water quality detecting device of automation collection, classification and Detection and record | |
| CN115491405A (en) | Microorganism detection device and microorganism detection method | |
| KR200395649Y1 (en) | The measuring apparatus for active sludge volume | |
| CN107811655A (en) | A kind of Urine specimens leave and take device | |
| JP5841684B1 (en) | BOD analyzer | |
| CN208659408U (en) | A kind of Urine specimens leave and take device | |
| CN110275039A (en) | Sample pipetting volume system and Ames test instrument |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |