CN115491405A

CN115491405A - Microorganism detection device and microorganism detection method

Info

Publication number: CN115491405A
Application number: CN202211362613.0A
Authority: CN
Inventors: 刘伟; 府岳; 孙志宏; 郁士林
Original assignee: Suzhou Nano Health Research Institute Co ltd
Current assignee: Suzhou Nano Health Research Institute Co ltd
Priority date: 2022-11-02
Filing date: 2022-11-02
Publication date: 2022-12-20

Abstract

The invention relates to a microorganism detection device and a microorganism detection method, and belongs to the technical field of microorganism detection. It mainly to in microbial detection, need prepare the diluent that has different dilutions, and the diluent of different ratios need utilize different sterilization straws to absorb, and this can cause the extravagant problem in a large number of straws, proposes following technical scheme: the method mainly comprises the steps of pretreatment, culture medium preparation, diluent sample preparation with different concentrations, inoculation and culture, and colony counting report generation and observation; the device comprises a diluent configuration mechanism, wherein the diluent configuration mechanism comprises a vertically corresponding proportioning barrel and a measuring barrel, the bottom end of the proportioning barrel is rotatably connected with the measuring barrel through a connecting arm, and a discharge port at the bottom end of the proportioning barrel is connected with a control valve. The design of diluent configuration mechanism is used in the microorganism detection method, the use of sterilization straws can be reduced in the process of configuring diluent with different concentrations, and the waste of straws is greatly reduced.

Description

Microorganism detection device and microorganism detection method

Technical Field

The invention relates to the technical field of microorganism detection, in particular to a microorganism detection device and a microorganism detection method.

Background

In the microbial detection, diluents with different dilutions need to be prepared, and diluents with different proportions need to be sucked by different sterilizing straws, which causes a great amount of waste of straws.

Disclosure of Invention

The present invention is directed to a microorganism detection apparatus and a microorganism detection method, which solve the problems described in the background art.

The technical scheme of the invention is as follows: a microorganism detection method mainly comprises the steps of pretreatment, culture medium preparation, diluent sample preparation with different concentrations, inoculation culture and colony count report generation and observation, and specifically comprises the following steps:

s1: the pretreatment comprises the preparation and disinfection of test instruments;

s2: preparing an agar culture medium, and cooling to 45 ℃;

s3: a diluent preparation mechanism is used for preparing 1;

s4: injecting the diluent with each concentration into different dishes, respectively adding about 15ml of agar culture medium, rotating the dishes to mix evenly, turning the dishes to the bottom upwards after the agar is solidified, and culturing in an incubator at 36 +/-1 ℃ for 48 +/-2 hours.

A microorganism detection device for the microorganism detection method of claim 1, comprising a diluent configuration mechanism, wherein the diluent configuration mechanism comprises a vertically corresponding proportioning barrel and a measuring barrel, the bottom end of the proportioning barrel is rotatably connected with the measuring barrel through a connecting arm, a discharge port at the bottom end of the proportioning barrel is connected with a control valve, and a piston capable of separating the internal space of the measuring barrel into specific volumes is arranged in the measuring barrel.

Preferably, the inner wall of the measuring cylinder is provided with scale marks.

Preferably, the lower surface of the piston is connected with a screw shaft, the bottom of the measuring cylinder is fixedly embedded with a screw sleeve, and the screw sleeve is in screw sleeve connection with the screw shaft.

Preferably, the proportioning barrel and the measuring barrel are provided with a plurality of barrels, the outer wall of each proportioning barrel is fixedly sleeved with a lantern ring, the upper lantern ring and the lower lantern ring are vertically adjacent and fixedly connected through a connecting rod, and the bottom end of the connecting rod which is located at the bottommost side is fixedly connected with a base.

Preferably, the outer wall of the measuring cylinder is fixedly sleeved with a liquid collecting bowl which is in a thin-wall ball shape and has an opening at the top end, and the liquid collecting bowl is used for collecting redundant diluent overflowing from the port of the measuring cylinder.

Preferably, an inner cavity flow channel is formed in the thin wall of the liquid collecting bowl, a first flow port communicated with the inner cavity flow channel is formed in the upper side of the inner wall of the liquid collecting bowl, and a second flow port communicated with the inner cavity flow channel is formed in the lower side of the inner wall of the liquid collecting bowl.

Compared with the prior art, the invention has the beneficial effects that:

(1): the design of the diluent preparation mechanism is used in the microorganism detection method, so that the use of sterilization straws can be reduced in the process of preparing diluents with different concentrations, and the waste of straws is greatly reduced;

(2): the design of diluent configuration mechanism is passed through in this application, and the diluent of different concentrations is from going up right side down the well in proper order, clearly visible, the problem of the wrong sample of record that probably appears when using proportion such as straw, test tube can effectively be avoided.

Drawings

FIG. 1 is a flow chart of a method for detecting microorganisms;

FIG. 2 is a schematic view of a diluent distribution mechanism of the microorganism detection apparatus;

fig. 3 is a cross-sectional view of the cartridge of fig. 2.

Reference numerals: a diluent preparation mechanism;

1. a proportioning barrel; 11. a control valve;

2. a connecting arm;

3. a measuring cylinder; 31. scale lines; 32. a screw shaft; 33. a threaded sleeve; 34. a liquid collection bowl; 341. a first circulation port; 342. a second flow port; 343. an inner cavity flow channel; 35. a piston;

4. a collar;

5. a connecting rod;

6. a base.

Detailed Description

The technical solution of the present invention is further explained with reference to the accompanying drawings and specific embodiments.

Example one

The invention provides a microorganism detection method, which is characterized in that: the method mainly comprises the steps of pretreatment, culture medium preparation, diluent sample preparation with different concentrations, inoculation and culture, and colony count report generation and observation, and specifically comprises the following steps:

s2: preparing an agar culture medium, and cooling to 45 ℃;

s3: preparing 1;

As shown in fig. 1-2, a microorganism detection device for the microorganism detection method comprises a diluent configuration mechanism a, wherein the diluent configuration mechanism a comprises a proportioning barrel 1 and a measuring barrel 3 which are vertically corresponding to each other, the bottom end of the proportioning barrel 1 is rotatably connected with the top end of a connecting arm 2 through a rotating shaft, and the bottom end of the connecting arm 2 is rotatably connected with the measuring barrel 3 through a rotating shaft. The discharge hole at the bottom end of the proportioning barrel 1 is connected with a control valve 11, and the control valve 11 can adopt an electromagnetic valve, a flow valve and the like. The piston 35 capable of separating the inner space of the measuring cylinder 3 into specific volumes is arranged in the measuring cylinder 3, the scale mark 31 is arranged on the inner wall of the measuring cylinder 3, and the volume above the piston 35 can be visually displayed according to the scale mark 31.

The lower surface of the piston 35 is connected with a screw shaft 32, the bottom of the measuring cylinder 3 is fixedly embedded with a screw sleeve 33, and the screw sleeve 33 is in screw sleeve connection with the screw shaft 32. When the screw shaft 32 is rotated, the screw shaft 32 can be driven to move up and down through the screw connection between the screw shaft 32 and the screw sleeve 33, so as to drive the piston 35 to move up and down, and thus the upper capacity of the piston 35 can be adjusted.

A ratio section of thick bamboo 1 and a measurement section of thick bamboo 3 all are provided with a plurality ofly, and a lantern ring 4 has all been cup jointed fixedly to the outer wall of every ratio section of thick bamboo 1, through connecting rod 5 fixed connection between the upper and lower adjacent lantern ring 4, is located the bottom fixedly connected with base 6 of the connecting rod 5 of bottommost side.

In this embodiment, the working principle of the diluent configuration mechanism a is as follows: firstly, injecting a sample and physiological saline into the uppermost single proportioning cylinder 1 according to the ratio of 1; in the above manner, 1ml of the 1 st 100 th dilution solution is taken out from the second proportioning cylinder 1 and poured into the third proportioning cylinder 1, and then 9ml of the normal saline is injected into the third proportioning cylinder 1 and mixed uniformly, so as to obtain the 1 st 1000 th dilution solution. Thus, three concentrations of the diluent samples were prepared.

And (3) placing the three plates below the three measuring cylinders 3 respectively, injecting diluent with corresponding concentration into each plate according to the mode, and then injecting a culture medium into each plate for inoculation and cultivation.

Example two

Compared with the first embodiment, the present invention further includes: as shown in fig. 2, a microorganism detection apparatus includes the following structure: the outer wall of the measuring tube 3 is fixedly sleeved with a liquid collecting bowl 34 which is in a thin-wall ball shape and has an opening at the top end, and the liquid collecting bowl 34 is used for collecting redundant diluent overflowing from the port of the measuring tube 3. An inner cavity flow channel 343 is formed in the thin wall of the liquid collecting bowl 34, a first flow port 341 communicated with the inner cavity flow channel 343 is formed in the upper side of the inner wall of the liquid collecting bowl 34, and a second flow port 342 communicated with the inner cavity flow channel 343 is formed in the lower side of the inner wall of the liquid collecting bowl 34.

The working principle of the embodiment is as follows: the embodiment is optimized on the basis of the first embodiment, and specifically, in the process of controlling the outflow of the diluent through the control valve 11, if the diluent overflows due to multiple flows, the overflowing diluent enters the liquid collecting bowl 34 through the top opening of the liquid collecting bowl 34 and is collected. When the measuring cylinder 3 is tilted, the opening of the measuring cylinder 3 is tilted downward, and at this time, the diluent in the liquid collecting bowl 34 flows along the inner wall of the liquid collecting bowl 34 and flows into the inner cavity flow channel 343 through the first flow port 341 and the second flow port 342, so as to prevent the diluent from being poured into the proportioning cylinder 1 together, and after the measuring cylinder 3 is reset, the diluent flows into the liquid collecting bowl 34 again through the second flow port 342 from the inner cavity flow channel 343.

In this embodiment, the liquid collection bowl 34 can be detachably connected with the measuring cylinder 3 in a manner of screwing threads and the like, and if the liquid collection bowl 34 is adopted, the diluent in the liquid collection bowl 34 can be cleaned only by detaching the liquid collection bowl 34 from the measuring cylinder 3; if the liquid collecting bowl 34 is integrally connected to the measuring tube 3, an outlet can be formed in the liquid collecting bowl 34, a sealing plug is additionally plugged into the outlet, and the diluent can be discharged through the outlet after the sealing plug is pulled out.

The embodiments of the present invention have been described in detail with reference to the drawings, but the present invention is not limited thereto, and various changes can be made within the knowledge of those skilled in the art without departing from the gist of the present invention.

Claims

1. A method for detecting a microorganism, comprising: the method mainly comprises the steps of pretreatment, culture medium preparation, diluent sample preparation with different concentrations, inoculation and culture, and colony count report generation and observation, and specifically comprises the following steps:

s2: preparing an agar culture medium, and cooling to 45 ℃;

s3: a diluent arrangement mechanism (a) is used for arranging diluents with the concentrations of 1;

2. The microorganism detection device for the microorganism detection method according to claim 1, comprising a diluent distribution mechanism (a), wherein the diluent distribution mechanism (a) comprises a vertically corresponding proportioning barrel (1) and a measuring barrel (3), the bottom end of the proportioning barrel (1) is rotatably connected with the measuring barrel (3) through a connecting arm (2), a control valve (11) is connected to a discharge port at the bottom end of the proportioning barrel (1), and a piston (35) capable of separating the internal space of the measuring barrel (3) into specific volumes is arranged in the measuring barrel (3).

3. A microorganism detection device according to claim 2, characterized in that the inner wall of the measuring cylinder (3) is provided with graduation marks (31).

4. The microorganism detection device according to claim 2, wherein a screw shaft (32) is connected to a lower surface of the piston (35), a screw sleeve (33) is fixedly embedded at the bottom of the measuring cylinder (3), and the screw sleeve (33) is spirally sleeved with the screw shaft (32).

5. The microorganism detection device according to claim 2, wherein a plurality of proportioning barrels (1) and measuring barrels (3) are provided, a lantern ring (4) is fixedly sleeved on the outer wall of each proportioning barrel (1), the upper and lower adjacent lantern rings (4) are fixedly connected through a connecting rod (5), and a base (6) is fixedly connected to the bottom end of the connecting rod (5) at the bottommost side.

6. The microorganism detection device according to claim 2, wherein a liquid collecting bowl (34) which is in a shape of a thin-walled ball and is open at the top end is fixedly sleeved on the outer wall of the measuring cylinder (3), and the liquid collecting bowl (34) is used for collecting excessive diluent overflowing from the port of the measuring cylinder (3).

7. The microorganism detection device according to claim 6, wherein an inner cavity flow channel (343) is formed inside the thin wall of the liquid collection bowl (34), a first flow port (341) communicated with the inner cavity flow channel (343) is formed on an upper side of an inner wall of the liquid collection bowl (34), and a second flow port (342) communicated with the inner cavity flow channel (343) is formed on a lower side of the inner wall of the liquid collection bowl (34).