CN107868748B - Ames tester - Google Patents

Abstract

The application provides an Ames tester, and relates to the technical field of Ames tests. It comprises the following steps: the culture dish storage and sorting device is arranged on the base and is configured to take out culture dishes which are stored in the culture dish storage and sorting device and are not added with various substances one by one, and the culture dishes are stored in the culture dish storage and sorting device after mixed liquid composed of various substances is added in each culture dish; the cover opening and closing station device is configured to drive the culture dish to move by utilizing the culture dish storage and sorting device so as to separate a dish plate and a dish cover of the culture dish, and can convey the dish plate of the culture dish to each preset station; the sample adding system is configured to mix a plurality of solutions and one sample, and convey mixed liquid formed after mixing into a dish at a mixed liquid adding station; and the shaking device is used for uniformly mixing the mixed liquid in the dish. According to the application, each stack of culture dishes in each sorting and accommodating cavity can be taken out one by one, and a plurality of substances can be added into each culture dish taken out one by one and then transferred to each storage and accommodating cavity.

Description

Ames tester

Technical Field

The application relates to the technical field of Ames test technology, in particular to an Ames tester.

Background

N.Ames et al have been strived for over ten years, and Salmonella back mutation assays (also known as Ames assays, ames assays) established and developed in 1975 have been widely used in countries around the world. The method is relatively quick, simple, convenient, sensitive and economical, and is suitable for testing mixtures and reflecting the comprehensive effect of various pollutants. The Ames test can be used to detect mutagenicity of food additives, cosmetics, etc., and thereby infer carcinogenicity thereof; the Ames test is used for detecting the mutagenicity of source water and drinking water, and a sanitation and safety disinfection measure which is more sanitary than that of the existing method is explored; or detecting mutagenicity of urban sewage and industrial wastewater, and tracking pollution sources by combining chemical analysis, so as to provide basis for researching control countermeasures; detecting mutagenicity of soil, sludge, industrial waste residue compost and waste ash to prevent damage to human beings through crops after the soil for life maintenance is polluted by the mutagenic substances; detecting mutagenicity of gaseous pollutants, and preventing the pollutants from potentially damaging human bodies through the atmosphere and respiration; the Ames test is used for researching the relation between the structure and the mutagenicity of the compound, and a theoretical basis is provided for synthesizing a novel compound which has no potential harm to the environment; detecting mutagenicity of the pesticide before and after microbial degradation, and knowing whether the pesticide has hidden danger to human beings in the metabolic process after application; also, ames test was used to screen anti-mutant, research and development of new anticancer drugs, and the like. The existing Ames test is that laboratory staff adopts a dropper to add reagents into a culture dish, so that the automation degree is low, the labor cost is high, certain experimental precision can be ensured, and the experimental result deviation is huge due to human error.

Disclosure of Invention

The present application has been made in view of the above problems, and has as its object to provide an Ames tester which overcomes or at least partially solves the above problems.

The application provides an Ames tester, bao Deban, on which a through hole is arranged to allow a culture dish to pass through;

a culture dish rack rotatably mounted on the bottom plate and having a plurality of accommodation cavities arranged in a circumferential direction of the bottom plate, each of the accommodation cavities extending in a vertical direction and each of the accommodation cavities being coaxial with the through hole when rotated above the through hole; and

a lifting mechanism having a carrying portion disposed below the through hole, the lifting mechanism being configured to:

the supporting part is moved upwards into the through hole to rotate to be coaxial with the through hole and accommodate

The culture dish in the accommodating cavity of at least one culture dish is positioned on the supporting part, and then the supporting part moves downwards,

and the upper surface of the lowest culture dish in the accommodating cavity is moved to be level with or lower than the upper surface of the bottom plate

The upper surface of the bottom plate is positioned so that the lowest culture dish in the accommodating cavity is sorted out; and/or

The carrier carrying one or more dishes is moved upward and a lower surface of a lowermost dish of the one or more dishes is moved to a position flush with or higher than an upper surface of the bottom plate so that the one or more dishes are stored in the accommodation chamber rotated to be coaxial with the through hole.

Further, the plurality of receiving cavities comprises at least one sorting receiving cavity and/or at least one storage receiving cavity, each sorting receiving cavity being configured to store a culture dish to be sorted, each storage receiving cavity being configured to store a culture dish to be stored.

Further, the culture dish storage and sorting device further comprises at least one collet disposed at a lower portion of each storage accommodating cavity, wherein each collet is configured to move under the effect of upward movement of the culture dish carried by the carrying portion so as to allow the culture dish carried by the carrying portion to move upward to the upper side thereof, and returns to the initial position after the culture dish carried by the carrying portion moves upward to the upper side thereof so as to carry the culture dish thereon.

Further, each of the holders includes at least three support columns, one end of each of which is rotatably installed at a lower portion of one of the storage accommodating chambers to be rotated upward when the culture dish carried by the carrier is moved upward, thereby allowing the culture dish carried by the carrier to be moved upward, and rotated downward to return to an original position after the culture dish carried by the carrier is moved upward, thereby supporting the culture dish with the stem thereof.

Further, the dish rack includes a plurality of rack bars extending in a vertical direction, and the plurality of rack bars define the plurality of receiving cavities.

Further, the culture dish rack further comprises a lower support plate frame and an upper support plate frame which are respectively arranged at two ends of each rack rod.

Further, the lifting mechanism further comprises a linear sliding table so as to drive the carrying part to move up and down.

Further, the carrying part is a bracket or a tray.

Further, the culture dish storage and sorting device further comprises a rotating device, wherein the rotating device is arranged below the bottom plate and is configured to drive the culture dish rack to rotate.

The application also provides an Ames tester, which comprises any one of the culture dish storage sorting device, wherein the culture dish storage sorting device is configured to take out culture dishes which are stored in the culture dish storage sorting device and are not added with various substances one by one, and after the various substances are added in each culture dish, the culture dish is stored in the culture dish storage sorting device.

According to the Ames tester provided by the application, each stack of culture dishes in each sorting accommodating cavity can be taken out one by one, and the taken out culture dishes can be transferred to each storage accommodating cavity after a plurality of substances are added in the culture dishes.

Drawings

Some specific embodiments of the application will be described in detail hereinafter by way of example and not by way of limitation with reference to the accompanying drawings. The same reference numbers will be used throughout the drawings to refer to the same or like parts or portions. It will be appreciated by those skilled in the art that the drawings are not necessarily drawn to scale. In the accompanying drawings:

FIG. 1 is a schematic block diagram of a culture dish storage sorting apparatus according to one embodiment of the present application;

FIG. 2 is a schematic block diagram of another view of the culture dish storage sorting apparatus of FIG. 1;

FIG. 3 is a schematic block diagram of an Ames tester according to one embodiment of the application;

FIG. 4 is a schematic partial block diagram of an Ames tester according to one embodiment of the application;

fig. 5 is a schematic partial block diagram of an Ames tester according to one embodiment of the present application.

Description of the embodiments

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present application more apparent, the following detailed description of the embodiments will be given with reference to the accompanying drawings. It should be noted that in the embodiments of the present application, some of the pipes are shown only in part in the drawings, and some of the pipes are not shown in the drawings.

Fig. 1 is a schematic block diagram of a dish storage sorting apparatus 200 according to one embodiment of the present application. As shown in fig. 1 and referring to fig. 2, an embodiment of the present application provides a dish storage sorting apparatus 200 for an amsi tester. The dish storage sorting apparatus 200 may include a base plate 210, a dish rack 220, and a lifting mechanism 230.

The bottom plate 210 may be a circular plate with a through hole 211 formed therein, and the through hole 211 may be slightly larger or larger than the diameter of the culture dish to allow the culture dish to pass therethrough.

The dish rack 220 is rotatably mounted to the base plate 210. The dish rack 220 may have a plurality of receiving cavities aligned in the circumferential direction of the bottom plate 210, each receiving cavity extending in the vertical direction and each receiving cavity being coaxial with the through hole 211 when rotated above the through hole 211. A stack of culture dishes can be placed in each receiving chamber.

The elevating mechanism 230 may have a carrying portion 231 disposed below the through hole 211, and the elevating mechanism 230 is configured to:

firstly, the carrying part 231 is moved upwards into the through hole 211, so that the culture dish in the accommodating cavity which is coaxial with the through hole 211 and accommodates at least one culture dish is positioned on the carrying part 231, then the carrying part 231 is moved downwards, and the upper surface of the lowest culture dish in the accommodating cavity is moved to be level with the upper surface of the bottom plate 210, so that the lowest culture dish in the accommodating cavity is sorted out; and/or

The carrier 231 carrying the one or more dishes is moved upward and the lower surface of the lowermost one of the one or more dishes is moved to a position flush with the upper surface of the bottom plate 210 or higher than the upper surface of the bottom plate 210 so that the one or more dishes are stored in the receiving chamber rotated to be coaxial with the through hole 211.

In some implementations of embodiments of the application, the plurality of receiving cavities includes at least one sorting receiving cavity and at least one storage receiving cavity, each sorting receiving cavity configured to store a culture dish to be sorted, each storage receiving cavity configured to store a culture dish to be stored.

When the sorting and accommodating device is particularly used, a stack of culture dishes are firstly placed in each sorting and accommodating cavity, and each culture dish is provided with a dish cover and a dish plate. When it is desired to take out the dishes one by one in one of the sorting accommodation chambers, the carrying part 231 is first moved upward into the through hole 211 such that the upper surface of the carrying part 231 is approximately flush with the upper surface of the bottom plate 210. The culture dish rack 220 is rotated so that the sorting accommodation chamber is rotated above the through hole 211, and the culture dishes in the sorting accommodation chamber are positioned on the carrying part 231 and do not fall down from the through hole 211 due to the carrying part 231. Then, the carrying portion 231 can be slowly lowered by the elevating mechanism 230. When the upper surface of the lowermost dish in the sorting accommodation chamber moves to a position flush with the upper surface of the bottom plate 210 or lower than the upper surface of the bottom plate 210 (preferably flush, slightly higher or slightly lower), the descent is stopped, and the dish rack 220 is rotated to prevent the dishes not to be taken out from falling down when the loading part 231 descends again. Then, the carrier 231 is further lowered to separate the extracted culture dish from the through hole 211 and the culture dish rack 220, so that the Ames tester can perform the subsequent operation.

After various substances are added to the removed culture dish, the culture dish needs to be stored. Specifically, the culture dish is placed on the supporting part 231 of the elevating mechanism 230, and the supporting part 231 carries the culture dish to move upward when the upper surface of the culture dish moves to a position flush with the upper surface of the bottom plate 210 or lower than the upper surface of the bottom plate 210 (preferably flush, slightly higher or slightly higher)

Below all, the ascent is stopped, and the dish rack 220 is rotated so that one storage accommodating chamber is rotated above the through hole 211. The lower surface of the dish is then moved to a position flush with the upper surface of the bottom plate 210 or above the upper surface of the bottom plate 210. Finally, the dish rack 220 is rotated to prevent the dishes in the storage accommodating chamber from falling down after the carrying part 231 is lowered. This allows the dishes to be stored in the storage compartment.

In this embodiment, since the culture dish storage sorter 200 has both the sorting accommodation chamber and the storage accommodation chamber, the Ames tester having the culture dish storage sorter 200 may have only one culture dish storage sorter 200, and the carrier 231 of the elevating mechanism 230 may not move after storing the culture dishes, and the culture dishes may be taken out when one sorting accommodation chamber rotates above the through hole 211. Preferably, in this embodiment, the number of sorting pockets and storage pockets is equal.

In other embodiments of the application, the culture dish storage sorting device 200 has only at least one sorting receiving chamber or only at least one storage receiving chamber, i.e. the culture dish storage sorting device 200 serves only for sorting or for storage purposes. The Ames tester may have two petri dishes storage sorter 200, one for sorting only, in which petri dishes to which no various substances have been added are placed, and the other for storing only, in which petri dishes to which various substances have been added are placed.

In some embodiments of the present application, the dish rack 220 may include a plurality of rack bars 221 extending in a vertical direction, an upper support plate rack 223, and a lower support plate rack 222. The plurality of hack levers 221 define a plurality of receiving chambers, for example, one receiving chamber is defined every four hack levers 221. A lower support plate bracket 222 and an upper support plate bracket 223 are respectively installed at both ends of each rack bar 221. Lifting and lowering device

The mechanism 230 may further include a linear sliding table to drive the carrying portion 231 to move up and down. The carrying portion 231 is preferably a bracket or a tray. A rotation device is further disposed below the bottom plate 210 and configured to rotate the culture dish rack 220.

In some embodiments of the present application, the dish storage and sorting apparatus 200 further includes at least one collet 240 disposed at a lower portion of each storage receiving cavity, each collet 240 being configured to move under the upward movement of the dishes carried by the carrying part 231 to allow the dishes carried by the carrying part 231 to move upward thereto, and to return to an initial position to carry the dishes thereon after the dishes carried by the carrying part 231 move upward thereto. This arrangement makes the storage step of the culture dish simpler, and when the culture dish is required to be stored after various substances are added in the extracted culture dish, a storage accommodating cavity is rotated above the through hole 211, and then the culture dish is placed on the supporting part 231 of the lifting mechanism 230 for supporting

The carrier 231 carries the culture dish to move upwards until the height of the carrier 231 is higher than that of the collet 240, the collet 240 allows the carrier and the culture dish to pass through, then returns to the initial position, the carrier 231 descends, and the culture dish can be located on the collet 240. Further, the underwire may also provide a gap between the stack of plates and the bottom plate 210 to facilitate removal of the plates by a user.

Specifically, each of the holders 240 includes at least three support columns, one end of each of which is rotatably installed at a lower portion of one storage receiving chamber to be rotated upward when the culture dish carried by the carrier 231 is moved upward, thereby allowing the culture dish carried by the carrier 231 to be moved upward and rotated downward to return to an original position after the culture dish carried by the carrier 231 is moved upward, thereby supporting the culture dish with its stem.

FIG. 3 is a schematic block diagram of an Ames test apparatus according to one embodiment of the present application, as shown in FIG. 3, and referring to FIGS. 4 and 5, the embodiment of the present application further provides an Ames test apparatus, which may include a base 100, a dish storage sorting device 200 according to any of the above embodiments, a cover opening/closing station device 300, and a sample loading system 400, so that the Ames test may be implemented

The automation is realized, and the experimental efficiency and the experimental precision are improved on the basis of reducing the labor cost.

The bottom plate 210 and the lifting mechanism 230 of the dish storage and sorting apparatus 200 are installed to the base 100, and the dish storage and sorting apparatus 200 may be configured to take out dishes stored therein without adding various substances one by one, and store the dishes therein after adding a mixed solution of various substances in each dish. That is to say that the culture dish storing and sorting device 200 in this embodiment comprises both a sorting receiving chamber and a storage receiving chamber.

The cover opening and closing station apparatus 300 may be configured to disengage the dish 710 and the dish cover 720 of the culture dish by the movement of the culture dish by the culture dish storage and sorting apparatus 200, and may transport the dish of the culture dish to each predetermined station. For example, the cover opening/closing station apparatus 300 may receive the culture dish at the dish cover opening/closing station, and then transport the dish to the mixed solution adding station, and further transport the dish of the culture dish back to the dish cover opening/closing station, etc.

The sample application system 400 may be configured to mix a plurality of solutions with a sample and deliver the mixed solution formed after mixing to a dish at a mixed solution addition station.

Specifically, in some embodiments of the present application, the cover opening and closing station apparatus 300 may include a stand, an upper tray 310, and a station carousel 320. The bracket is fixed to the base 100. The upper plate 310 is rotatably mounted to the bracket and has a plurality of first stepped holes. The station carousel 320 is rotatably mounted to the support and below the upper plate 310, and the station carousel 320 has a plurality of second stepped holes thereon. Preferably, the number of the first stepped holes and the second stepped holes are equal.

The lifting mechanism 230 of the dish storage sorter 200 is also configured to: when a first stepped hole and a second stepped hole are positioned at the same axial position, the carrying part 231 carrying the culture dish downwards moves from the upper part of the upper plate 310, so that the carrying part 231 sequentially passes through the first stepped hole and the second stepped hole, and in the process of moving the carrying part 231, the dish cover 720 of the culture dish is positioned in the first stepped hole and the dish 710 of the culture dish is positioned in the second stepped hole, thereby separating the dish cover and the dish 710 of the culture dish; and when the first stepped hole of one carrying dish cover and the second stepped hole of one carrying dish are at the coaxial position, the carrying part 231 moves upwards from the lower direction of the station turntable 320, so that the carrying part 231 sequentially passes through the second stepped hole and the first stepped hole, and in the moving process of the carrying part 231, the dish of the culture dish moves upwards to be matched with the dish cover of the culture dish, so that the dish cover of the culture dish is installed on the dish.

That is, after the lifting mechanism 230 takes out the culture dishes one by one, the lifting mechanism 230 continues to descend, sequentially passes through the first step hole and the second step hole at the dish cover opening and closing station, so that the dish cover 720 is located on the step surface of the first step hole, the dish 710 is located on the step surface of the second step hole, and the dish is separated from the dish cover. Then, the station turntable 320 rotates to transport the dish to the mixed solution adding station, the sample adding system 400 transports the mixed solution into the dish 710, and then the station turntable 320 brings the dish back to the dish cover opening and closing station. At this time, the tray 231 under the station turntable 320 moves upward to set the dish cover on the dish, and continues to move upward to store the dishes in the dish storage sorter 200.

In the embodiment of the present application, the station turntable 320 and the upper plate 310 need to rotate synchronously to ensure the synchronicity of the dish and the dish cover of the same dish. In this case, the dish cover opening and closing station and the mixed liquid adding station can synchronously operate, so that when mixed liquid is added, the dish can be separated from or combined with the dish cover by the other dish. In addition, the diameter of the station carousel 320 is greater than that of the upper plate 310 to prevent inconvenience in adding the mixed liquor in the dish due to shielding of the station carousel 320 by the upper plate 310.

In some embodiments of the present application, the loading system 400 may include a plurality of mixing vials 410, a first drive device, a plurality of sample storage vials 420, a sampling device, a plurality of solution storage containers 440, and a solution retrieval device.

The first drive means are configured to move each mixing tube 410 to at least a first station and a second station, with the first station and the second station being in two positions, respectively.

A plurality of sample storage tubes 420 are each used to hold a plurality of samples.

The sampling device may be configured to withdraw a sample from one of the sample storage tubes 420 and transfer the withdrawn sample into the mixing tube 410 at the first station. The sampling device may also be configured to withdraw the mixed liquor from one of the mixing tubes 410 and then deliver the withdrawn mixed liquor to a dish in the mixed liquor addition station.

The plurality of solution storage containers 440 are respectively used to hold a plurality of solutions.

The solution withdrawal device is configured to withdraw and deliver the solutions in the plurality of solution storage containers 440 in a predetermined order into the mixing tube 410 at the second station.

In this embodiment, the first drive means is further configured to move each mixing tube 410 to a first station where it receives a sample and then to a second station where it receives multiple solutions. With a plurality of mixing vials 410 and first drive means, one mixing vial 410 may be moved to a first station and a second station to receive multiple solutions and samples in one sample storage vial 420 to form one mixed solution. The other mixing tube 410 may then be moved to the first station and the second station to receive the plurality of samples and the other sample within the other sample storage tube 420 to form the other mixing fluid.

Multiple mixing tubes 410 may form multiple mixtures. In this embodiment, the first drive means is further configured to sequentially move a plurality of adjacent mixing vials 410 to a first station where the same sample is received, with the difference that the received samples are not uniform in size to form a sample gradient; and then to a second station to receive a plurality of solutions. The use of multiple mixing vials 410 and first drive means significantly improves experimental efficiency.

In some embodiments of the present application, a plurality of sample storage tubes 420 are spaced apart along a circumferential direction. The sampling device may include a sampling syringe, a first rotational elevator 431, and a first plunger pump 432. The first rotary elevating means 431 is configured to at least rotate the sampling needle above the mixing tube 410 or above each sample storage tube 420 at the first station and to move the sampling needle up and down. The first plunger pump 432 is in communication with the sampling syringe via tubing to draw in a sample from one of the sample storage tubes 420 and to expel the sample from the sampling syringe into the mixing tube 410 at the first station.

When it is desired to withdraw a sample from a sample storage tube 420, the sampling needle is rotated to above the sample storage tube 420, the sampling needle is lowered into the sample storage tube 420 below the solution, and the first plunger pump 432 is controlled to operate to allow the sample from the sample storage tube 420 to enter the sampling needle. Next, the sampling needle is raised and rotated to be rotated above the mixing tube 410 at the first station, then the sampling needle is lowered, and finally the sample introduced into the sampling needle is discharged from the sampling needle into the mixing tube 410 at the first station, thereby completing the sample extraction.

In some embodiments of the present application, the solution taking-out device includes a second rotary elevating device 451, a plurality of solution taking needle pipes 452, a second plunger pump 453, and a first flow path switching device. The second rotary elevating means 451 is configured to rotate each of the liquid-taking needle tubes 452 above the mixing tube 410 or above one of the solution storage containers 440 at the second station, and is configured to move each of the liquid-taking needle tubes 452 up and down. A second plunger pump 453 communicates with each of the tapping needle tubes 452 via tubing. The first flow switching device is configured to controllably communicate a liquid withdrawal syringe 452 with the second plunger pump 453 to controllably cause the second plunger pump 453 to cause each liquid withdrawal syringe 452 to draw solution from a solution storage container 440 and to cause solution entered into the liquid withdrawal syringe 452 to be expelled from the liquid withdrawal syringe 452 into the mixing tube 410 at the second station.

When multiple solutions need to be withdrawn, two ways can be used: in one embodiment, the plurality of access syringes 452 may be positioned above the plurality of solution storage containers 440 and then the plurality of access syringes 452 may be lowered into the plurality of solution storage containers 440. The first flow path switching device first connects one of the liquid taking needle tubes 452 to the second plunger pump so that the liquid taking needle tube 452 sucks in the solution. The second flow path switching means then causes the other of the liquid taking needle 452 to be in communication with the second plunger pump so as to draw in the solution from the liquid taking needle 452. Then, the second flow path switching device connects the third liquid taking needle tube 452 to the plunger pump, so that the liquid taking needle tube 452 sucks the solution. ....... Until the last liquid taking needle tube 452 is sucked with the corresponding solution. The plurality of liquid taking needle tubes 452 are lifted and rotated, so that the plurality of liquid taking needle tubes 452 are sequentially rotated above the mixing test tube 410 at the second station, and when a certain liquid taking needle tube 452 is above the mixing test tube 410, the first flow switching device conducts the liquid taking needle tube 452 and the second plunger pump 453, so that the solution in the liquid taking needle tube 452 is discharged to the mixing test tube 410 at the second station. In a second mode, the plurality of access syringes 452 may be positioned above the plurality of solution storage containers 440 and then the plurality of access syringes 452 may be lowered into the plurality of solution storage containers 440. The first flow path switching device first connects one of the liquid taking needle tubes 452 to the second plunger pump so that the liquid taking needle tube 452 sucks in the solution. The plurality of liquid taking needle tubes 452 are lifted and rotated, so that the liquid taking needle tubes 452 for sucking the solution are rotated to the upper part of the mixing test tube 410 at the second station, and the solution in the liquid taking needle tubes 452 is discharged to the mixing test tube 410 at the second station. The plurality of sampling syringes are then rotated such that the plurality of access syringes 452 are positioned above the plurality of solution storage containers 440, and the plurality of access syringes 452 are then lowered into the plurality of solution storage containers 440. The first flow path switching device connects the other liquid taking needle 452 to the second plunger pump so that the liquid taking needle 452 sucks in the solution. The plurality of liquid taking needle tubes 452 are lifted and rotated, so that the liquid taking needle tubes 452 for sucking the solution are rotated to the upper part of the mixing test tube 410 at the second station, and the solution in the liquid taking needle tubes 452 is discharged to the mixing test tube 410 at the second station. ....... Until all of the solution in the solution storage container 440 has been removed and placed into the mixing tube 410 at the second station.

In some embodiments of the application, the sampling device may further comprise a sample presentation needle and a second flow path switching device. The sample feeding needle tube is arranged on the first rotary lifting device 431, so as to rotate to the upper part of the mixing test tube 410 at the first station under the drive of the first rotary lifting device 431, and to perform lifting movement under the drive of the first rotary lifting device 431; and the sample presentation needle communicates with the first plunger pump 432 via tubing to draw the mixed fluid from the mixing tube 410 at the first station. The second flow path switching device is configured to controllably communicate the sample presentation needle with the first plunger pump 432 or to communicate the sample presentation needle with the first plunger pump 432. The first plunger pump is further configured to discharge the mixed liquid introduced into the sample-feeding needle tube into a dish of the culture dish.

When each mixing tube 410 receives a sample at the first station, moves to the second station, and returns to the first station immediately after receiving multiple solutions, the sample feeding needle tube can be moved up and down in a reciprocating manner to form a relatively uniform mixture of multiple substances in the mixing tube 410, and then the mixture in the mixing tube is transferred to a dish of one or more culture dishes.

In some embodiments of the application, the sample application system 400 further comprises a wash tank. The inlet of the second flow path switching device is also in controlled communication with the air compressor and the water supply pump via a valve device. The water supply pump is also used to provide cleaning solution to the cleaning tank, for example, the inlet of the water supply pump is controllably connected to the water tank or the alcohol reservoir. The first rotary lifting device 431 is configured to drive the sampling needle tube and the sample feeding needle tube to rotate to the upper side of the cleaning tank and then descend into the cleaning tank. When cleaning is needed, the first rotary lifting device 431 can enable the sampling needle tube and the sample feeding needle tube to be located above the cleaning tank, and the water supply pump can respectively inject water into the cleaning tank and the needle tube to clean the inside and the outside of the needle tube. Then, the water supply pump injects alcohol into the cleaning tank and the needle tube, and finally, the air compressor can inject air into the needle tube so as to clean the needle tube.

In some embodiments of the present application, a plurality of mixing vials 410 are spaced apart along a circumferential direction. The first driving means may comprise: a rotating cylinder to which a plurality of mixing tubes 410 are mounted; and the driving motor is configured to drive the rotary column body to perform rotary motion.

In some embodiments of the application, the Ames tester may further include a shaking device 500 for further homogenizing the mixture in the dish. Specifically, the shaking device 500 includes a base, a driving wheel, a driven wheel, a shaking frame, a lifting device and a carrying tray. The base is mounted to the base 100. The driving wheel and the driven wheel are both rotatably arranged on the base. The shaking-up frame is rotatably arranged on the driving wheel and the driven wheel at two positions of the shaking-up frame respectively.

In particular, in this embodiment, the base, the driving wheel, the driven wheel and the shaking frame together constitute a crank-rocker mechanism or a parallelogram mechanism. Further, the shaking-up frame comprises a base plate and a side plate, wherein the upper end of the side plate is arranged on one side surface of the base plate. The base plate is rotatably mounted to the driving wheel and the driven wheel at two portions of the lower surface thereof, respectively. For example, the substrate is provided with turning holes at two portions of the lower surface thereof; the shaking device 500 further comprises two rollers respectively mounted on the upper surfaces of the driving wheel and the driven wheel and respectively positioned in the two rotating holes. The lifting device is arranged on the side plate. The carrying tray is used for placing the culture dish and is arranged on the lifting device so as to carry out lifting motion under the driving of the lifting device. The carrying tray is preferably a suction cup. The lifting device can at least enable the dish plate of the culture dish to be separated from the second step hole so as to prevent the second step hole from obstructing the movement of the dish plate in the process of rotating. In order to better and more accurately move the carrier tray to a predetermined position for carrying the dish, the shaking apparatus 500 further comprises a position detection device configured to detect whether the shaking frame is in or back to the initial position. For example, the position detection device includes a signal sheet mounted at an edge of the substrate and a sensor mounted on the base.

In some embodiments of the present application, the Ames tester may further include a coding device 600 configured to provide a mark on the outer surface of the sidewall or the outer surface of the bottom wall of the dish at the coding station to prevent confusion in the placement of the mark on the dish cover. Specifically, the coding device 600 may include a code base, at least one slider, at least one coding pen, and a cylinder. The dock is mounted to the base 100. At least one slider is slidably mounted on the dock. Each code printing pen is fixed on one sliding block so as to stretch and retract along with the sliding block, and therefore marks are arranged on the outer surface of the side wall of the dish at the code printing station. The cylinder is configured to controllably extend and retract a stylus with each slider mounted on the slider. Further, the base comprises an inclined plate and a supporting plate arranged below the inclined plate. At least one slider is mounted on the inclined plate for telescopic movement in a direction parallel to the inclined plate. The pen point of each code printing pen faces obliquely downwards so as to better set the marks on the side wall of the dish.

The foregoing is merely a preferred embodiment of the present application and it should be noted that any modifications and adaptations to those skilled in the art should and have been made without departing from the principles of the present application.

Claims (6)

1. An ames tester, characterized in that: comprises a base, a culture dish storage and sorting device, a cover opening and closing station device, a sample adding system and a shaking device;

the culture dish storage and sorting device is arranged on the base and is configured to take out the culture dishes which are stored in the culture dish storage and sorting device and are not added with various substances one by one, and after mixed liquid composed of various substances is added into each culture dish, the culture dish is stored in the culture dish storage and sorting device; comprises a bottom plate, a culture dish rack, a lifting mechanism, at least one collet and a rotating device;

the bottom plate is provided with a through hole to allow the culture dish to pass through;

the culture dish rack is rotatably arranged on the bottom plate and is provided with a plurality of accommodating cavities arranged along the circumferential direction of the bottom plate, each accommodating cavity extends along the vertical direction, and each accommodating cavity is coaxial with the through hole when rotating above the through hole; the plurality of accommodating cavities comprise at least one sorting accommodating cavity and at least one storage accommodating cavity, each sorting accommodating cavity is configured to store culture dishes to be sorted, and each storage accommodating cavity is configured to store culture dishes to be stored;

the lifting mechanism is provided with a carrying part arranged below the through hole, and the lifting mechanism is configured to: firstly, the carrying part is moved upwards into the through hole, so that the culture dish in the accommodating cavity which is coaxial with the through hole and accommodates at least one culture dish is positioned on the carrying part, then, the carrying part is moved downwards, and the upper surface of the lowest culture dish in the accommodating cavity is moved to be level with the upper surface of the bottom plate, so that the lowest culture dish in the accommodating cavity is sorted out; and moving the carrying portion carrying one or more dishes upward and moving a lower surface of a lowermost one of the one or more dishes to a position flush with or higher than an upper surface of the bottom plate so that the one or more dishes are stored in the accommodation chamber rotated to be coaxial with the through hole; the lifting mechanism further comprises a linear sliding table so as to drive the supporting part to move up and down;

at least one shoe disposed at a lower portion of each storage accommodating chamber, each shoe configured to move under the upward movement of the culture dish carried by the carrying part to allow the culture dish carried by the carrying part to move upward to above the same, and to return to an initial position after the culture dish carried by the carrying part moves upward to above the same to carry the culture dish thereon; each of the shoe includes: at least three support columns, one end of each of which is rotatably installed at a lower portion of one of the storage accommodating chambers to be rotated upward when the culture dish carried by the carrying part moves upward, thereby allowing the culture dish carried by the carrying part to move upward above it, and rotated downward to return to an initial position after the culture dish carried by the carrying part moves upward above it, thereby supporting the culture dish with the stem thereof;

the rotating device is arranged below the bottom plate and is configured to drive the culture dish rack to rotate;

the cover opening and closing station device is configured to drive the culture dish to move by utilizing the culture dish storage and sorting device so as to separate a dish plate and a dish cover of the culture dish, and the dish plate of the culture dish can be conveyed to each preset station; comprises a bracket, an upper disc and a station turntable;

the bracket is fixed on the base;

the upper disc is rotatably arranged on the bracket and provided with a plurality of first step holes;

the station turntable is rotatably arranged on the bracket and is positioned below the upper disc and provided with a plurality of second step holes;

the lifting mechanism is further configured to: when one first step hole and one second step hole are positioned at the coaxial position, the carrying part carrying the culture dish downwards moves from the upper part of the upper plate, so that the carrying part sequentially passes through the first step hole and the second step hole, and in the moving process of the carrying part, the dish cover of the culture dish is positioned in the first step hole and the dish plate of the culture dish is positioned in the second step hole, so that the dish cover and the dish plate of the culture dish are separated; when the first step hole of one carrying dish cover and the second step hole of one carrying dish disk are positioned at the coaxial position, the carrying part moves upwards from the lower direction of the station turntable, so that the carrying part sequentially passes through the second step hole and the first step hole, and in the moving process of the carrying part, the dish disk of the culture dish moves upwards to be matched with the dish cover of the culture dish, so that the dish cover of the culture dish is installed on the dish disk;

the sample adding system is configured to mix a plurality of solutions with one sample, and convey mixed liquid formed after mixing into a dish at a mixed liquid adding station; comprises a plurality of mixing test tubes, a first driving device, a plurality of sample storage test tubes, a sampling device, a plurality of solution storage containers and a solution taking device;

the mixing test tubes are arranged at intervals along a circumferential direction;

the first driving device is configured to at least enable each mixing test tube to move to a first station and a second station, and the first station and the second station are respectively located at two positions; or is configured to move each of the mixing vials to a first station where one sample is received and then to a second station where multiple solutions are received; or is configured to move a plurality of adjacent mixing test tubes to a first station in sequence, and receive the same sample at the first station, wherein the difference is that the received samples are inconsistent so as to form a sample gradient; the first driving device includes: a rotating cylinder, wherein a plurality of mixing test tubes are arranged on the rotating cylinder; and a driving motor configured to drive the rotary cylinder to perform a rotary motion;

the plurality of sample storage test tubes are used for containing various samples and are arranged at intervals along a circumferential direction;

the sampling device is configured to take out a sample from one of the sample storage tubes and convey the taken sample into the mixing tube at the first station; or taking out the mixed liquid from one of the mixing test tubes, and then conveying the taken mixed liquid into a dish of a mixed liquid adding station; the sampling device comprises: sampling needle tube; the first rotary lifting device is configured to drive the sampling needle tube to at least rotate to the position above the mixed test tube at the first station or the position above each sample storage test tube, and is configured to drive the sampling needle tube to perform lifting movement; a first plunger pump in communication with said sampling needle by tubing to draw in a sample from one of said sample storage tubes and to expel a sample from said sampling needle into said mixing tube at a first station; the sample feeding needle tube is arranged on the first rotary lifting device, is driven by the first rotary lifting device to rotate to the position above the mixing test tube at the first station, and is driven by the first rotary lifting device to perform lifting movement; the sample feeding needle tube is communicated with the first plunger pump through a pipeline so as to absorb mixed liquid from the mixed test tube at the first station;

a second flow path switching device configured to controllably conduct the sample feeding needle tube and the first plunger pump, or conduct the sampling needle tube and the first plunger pump, the first plunger pump further configured to discharge the mixed liquid entering the sample feeding needle tube into a dish of a culture dish;

the plurality of solution storage containers are used for containing a plurality of solutions;

the solution taking-out device is configured to take out and convey the solutions in the plurality of solution storage containers to the mixing test tube at the second station in a predetermined order; a solution extraction device comprising: a liquid taking needle tube; the second rotary lifting device is configured to drive each liquid taking needle tube to rotate to the position above a mixing test tube at a second station or the position above a solution storage container, and is configured to drive each liquid taking needle tube to perform lifting movement; the second plunger pump is communicated with each liquid taking needle tube through a pipeline; a first flow path switching device configured to controllably communicate one of said liquid-taking needle tubes and said second plunger pump to controllably cause said second plunger pump to cause each of said liquid-taking needle tubes to draw in solution from one of said solution storage containers and to cause solution entered into said liquid-taking needle tube to be expelled from said liquid-taking needle tube into said mixing tube at a second station;

the shaking device is used for enabling the mixed liquid in the dish to be further and uniformly mixed.

2. An Ames tester as claimed in claim 1 wherein: the culture dish rack comprises a plurality of hack levers extending along the vertical direction, and the plurality of hack levers define a plurality of accommodating cavities.

3. An Ames tester as claimed in claim 2 wherein: the culture dish rack also comprises a lower support plate rack and an upper support plate rack which are respectively arranged at two ends of each rack rod.

4. An Ames tester as claimed in claim 1 wherein: the station turntable and the upper disc synchronously rotate;

and the diameter of the station turntable is larger than that of the upper disc.

5. An Ames tester as claimed in claim 1 wherein: the sample addition system further comprises: the inlet of the second flow path switching device is also in controlled communication with an air compressor and a water supply pump through a valve device, and the water supply pump can be used for supplying cleaning liquid to the cleaning tank;

the first rotary lifting device is configured to drive the sampling needle tube and the sample feeding needle tube to rotate to the upper part of the cleaning tank and then descend into the cleaning tank;

when cleaning is needed, the first rotary lifting device can enable the sampling needle tube and the sample feeding needle tube to be located above the cleaning tank, the water supply pump can respectively inject water into the cleaning tank and the needle tube so as to clean the inside and the outside of the needle tube, then, the water supply pump can inject alcohol into the cleaning tank and the needle tube, and finally, the air compressor can inject air into the needle tube so as to clean the needle tube.

6. An Ames tester as claimed in claim 1 wherein: the device also comprises a coding device which is configured to set marks on the outer surface of the side wall or the outer surface of the bottom wall of the dish at the coding station.