CN204848870U - Sample taking device - Google Patents

Sample taking device Download PDF

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Publication number
CN204848870U
CN204848870U CN201520475791.3U CN201520475791U CN204848870U CN 204848870 U CN204848870 U CN 204848870U CN 201520475791 U CN201520475791 U CN 201520475791U CN 204848870 U CN204848870 U CN 204848870U
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China
Prior art keywords
sampling
valve
line
steam
sample
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CN201520475791.3U
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Chinese (zh)
Inventor
陈晨晨
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Zhongsheng tianxinhe (Wuxi) Biotechnology Co., Ltd
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Beijing Tocell Biotechnology Co Ltd
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Abstract

The utility model provides a sample taking device, include: sample valve, sampling bottle, sample pipeline, admission line, air filter disc and steam conduit, wherein, the first end of sample valve links to each other with the sample connection of retort, the second end of sample valve passes through the sample pipeline with the sampling bottle links to each other, the third of sample valve end with the admission line links to each other, the air filter disc sets up on the admission line, the air filter disc can to input compressed air in the admission line, so that the sample valve with the liquid of remaining in the sample pipeline is discharged, the steam conduit connect in the air filter disc with admission line between the sample valve, the input can be right in the steam conduit the admission line the sample valve with the sample pipeline carries out the steam of sterilizing, the utility model provides a sample taking device has solved among the present sample taking device and to have retained the technical problem that the degree of accuracy that cell liquid led to the fact sample next time reduces because of the sample pipeline.

Description

Sampling unit
Technical field
The utility model relates to a kind of sampling system, particularly the sampling unit that samples of a kind of noresidue.
Background technology
Cell cultures is requisite process in biotechnology, a large amount of cells or its meta-bolites can be obtained by cell cultures, wherein, cell cultures is generally carried out in retort, need to carry out sampling analysis to the enchylema in retort in cell cultivation process, such as, the isoparametric detection of cell viability, cell density and glucose concn is carried out to enchylema; And morphologic observation is carried out to cell, the parameter related in cell cultivation process is adjusted by the analysis detecting data and morphologic observation, (dissolvedoxygen is called for short: the DO) parameter such as value and nutrient solution concentration for such as air flow, temperature value, pH value, dissolved oxygen.
At present, when enchylema in retort is sampled, sampled by sampling unit, sampling unit comprises: sampling valve and sampling jug, the first end of sampling valve is connected with the thief hole of retort, second end of sampling valve is connected with sampling jug by sampling line, 3rd end of sampling valve is connected with steam-pipe, wherein, sampling line is connected with blow-off line, before sampling, the steam control valve that steam-pipe is provided with and the wash water valve that blow-off line is provided with are opened, steam is through steam-pipe, sampling valve, sampling line and blow-off line carry out sterilizing to sampling unit, sampling is started after sterilizing, during sampling, the steam control valve that steam-pipe is provided with and the wash water valve that blow-off line is provided with are closed, enchylema in retort enters sampling jug by sampling valve through sampling line.
But after existing sampling unit sampling, can remain a part of enchylema in sampling line, when lower sub-sampling, the enchylema of this residual cell liquid meeting and lower sub-sampling mixes, thus can reduce the accuracy of lower sub-sampling.
Utility model content
The utility model provides a kind of sampling unit, solves in existing sampling unit because sampling line retains the technical problem that enchylema causes the accuracy of lower sub-sampling to reduce.
The utility model provides a kind of sampling unit, comprising:
Sampling valve, sampling jug, sampling line, intake ducting, air filter disc and steam-pipe;
Wherein, the first end of described sampling valve is connected with the thief hole of retort, and the second end of described sampling valve is connected with described sampling jug by described sampling line, and the 3rd end of described sampling valve is connected with described intake ducting;
Described air filter disc is arranged on described intake ducting, and described air filter disc to input compressed-air in described intake ducting, can be discharged to make the debris in described sampling valve and described sampling line;
Described steam-pipe is connected to the intake ducting between described air filter disc and described sampling valve, and in described steam-pipe, input can carry out the steam of sterilizing to described intake ducting, described sampling valve and described sampling line.
In embodiment of the present utility model, described intake ducting is provided with intake valve, and on the intake ducting of described intake valve between described air filter disc and described steam-pipe.
In embodiment of the present utility model, described steam-pipe is provided with steam control valve.
In embodiment of the present utility model, the first end of described sampling valve is connected by sterile connector with described retort thief hole.
In embodiment of the present utility model, described sampling line arranges connection valve, described sampling line is communicated with described sampling jug by described connection valve.
In embodiment of the present utility model, the sampling line between described connection valve and the 3rd end of described sampling valve connects blow-off line, and described blow-off line is provided with wash water valve.
In embodiment of the present utility model, described sampling line and described blow-off line are plastic conduit.
The sampling unit that the utility model provides, be connected with retort thief hole by the first end of described sampling valve, second end of described sampling valve is connected with described sampling jug by sampling line, 3rd end of described sampling valve is connected with intake ducting, described intake ducting is provided with air filter disc, described air filter disc can make the debris in described sampling valve and described sampling line discharge to blowing compressed air in described intake ducting, make to there is not debris in sampling line, descend the enchylema of sub-sampling gained just can be more accurate like this, reflect the cultivation situation in retort truly, solve in existing sampling unit because sampling line retains the technical problem that enchylema causes the accuracy of lower sub-sampling to reduce, and the intake ducting between described air filter disc and described sampling valve connects steam-pipe, the steam inputted in described steam-pipe is direct to described intake ducting before sampling, described sampling valve and described sampling line carry out sterilizing, whole sampling process simple operation, security is high.
Accompanying drawing explanation
In order to be illustrated more clearly in the utility model embodiment or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, apparently, accompanying drawing in the following describes is embodiments more of the present utility model, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is the structural representation of the utility model sampling unit;
Fig. 2 is the another structural representation of the utility model sampling unit.
Embodiment
For making the object of the utility model embodiment, technical scheme and advantage clearly, below in conjunction with the accompanying drawing in the utility model embodiment, technical scheme in the utility model embodiment is clearly and completely described, obviously, described embodiment is the utility model part embodiment, instead of whole embodiments.Based on the embodiment in the utility model, those of ordinary skill in the art are not making the every other embodiment obtained under creative work prerequisite, all belong to the scope of the utility model protection.
The sampling unit that the present embodiment provides, for sampling, specifically may be used for sampling cultured cells liquid in retort, can also be used for sampling when detecting other liquid samplings.
Fig. 1 is the structural representation of the utility model sampling unit, as shown in Figure 1, sampling unit comprises: sampling valve 2, sampling jug 3, sampling line C, intake ducting A, air filter disc 1 and steam-pipe B, wherein, the first end 2b of sampling valve 2 is connected with retort thief hole (not shown), second end 2c of sampling valve 2 is connected with sampling jug 3 by sampling line C, 3rd end 2a of sampling valve 2 is connected with intake ducting A, wherein, the first end 2b of sampling valve 2, be interconnected between second end 2c and the 3rd end 2a, in actual applications, for the ease of sampling, as shown in Figure 1, the the second end 2c be connected with sampling jug 3 is generally positioned at the bottom of sampling valve 2, 3rd end 2a is positioned at the top of sampling valve 2, in the present embodiment, the first end 2b of sampling valve 2, second end 2c and the 3rd end 2a is only in order to express easily, be used in reference to certain port of sampling valve 2, and be not used in the port limiting sampling valve 2, in the present embodiment, intake ducting A is provided with air filter disc 1, air filter disc 1 can make the debris in sampling valve 2 and sampling line C discharge to blowing compressed air in intake ducting A, after so every sub-sampling, in intake ducting A, pressurized air is blown into by the effect of air filter disc 1, the enchylema remained in sampling valve 2 and sampling line C is discharged under compressed-air actuated effect, then there is no debris in sampling valve 2 and sampling line C, just can not impact sampling result during lower sub-sampling, in the present embodiment, sterilizing must be carried out to sampling unit before every sub-sampling, in the present embodiment, be specially, intake ducting A between air filter disc 1 and sampling valve 2 connects steam-pipe B, the steam-energy inputted in steam-pipe B is to intake ducting A, sampling valve 2 and sampling line C carry out sterilizing, wherein sampling jug 3 can carry out sterilizing separately, after sterilizing terminates, stop input steam, start sampling, in the present embodiment, during sampling, sampling liquid enters sampling jug 3 by the first end 2b of sampling valve 2 and the second end 2c through sampling line C, after sampling, open air filter disc 1 and be blown into pressurized air in intake ducting A, enchylema residual in sampling valve 2 and sampling line C is discharged under compressed-air actuated effect, it should be noted that, in sampling process, in order to sample fast, also can open air filter disc 1 when sampling and be blown into pressurized air in intake ducting A, liquid in such sampling line can enter sampling jug 3 rapidly.
The sampling unit of the present embodiment, be connected with retort thief hole by the first end of sampling valve, second end of sampling valve is connected with described sampling jug by sampling line, 3rd end of sampling valve is connected with intake ducting, intake ducting is provided with air filter disc, described air filter disc can make the debris in sampling valve and described sampling line discharge to blowing compressed air in described intake ducting, make there is not residual enchylema in sampling line, during lower sub-sampling, the enchylema of sampling gained just can be more accurate, reflect the cultivation situation in retort truly, solve in existing sampling unit because sampling line retains the technical problem that enchylema causes the accuracy of lower sub-sampling to reduce, and the intake ducting between described air filter disc and described sampling valve connects steam-pipe, the steam inputted in described steam-pipe is direct to described intake ducting before sampling, described sampling valve and described sampling line carry out sterilizing, whole sampling process simple operation, security is high.
Fig. 2 is the another structural representation of the utility model sampling unit, on the basis of above-described embodiment, in the present embodiment, as shown in Figure 2, intake ducting A is provided with intake valve T1, and on the intake ducting A of intake valve T1 between air filter disc 1 and steam-pipe B, intake valve T1 enters intake ducting A for the pressurized air controlled in air filter disc 1, such as, intake valve T1 opens, pressurized air then in air filter disc 1 enters intake ducting A and removes the debris in sampling valve 2 and sampling line C, when sampling or sterilizing, then intake valve T1 closes, pressurized air in air filter disc 1 can not enter in intake ducting A, simultaneously, in the present embodiment, intake valve T1 can also control the compressed-air actuated flow of the input in intake ducting A, namely as required, the air flow quantity size in intake ducting A can be regulated by intake valve T1.
Further, for the ease of controlling the steam inputted in steam-pipe B, in the present embodiment, steam-pipe B is provided with steam control valve T2, steam control valve T2 can control the steam in steam-pipe, such as, steam control valve T2 opens, the steam then inputted enters intake ducting A through steam-pipe B, in sampling valve 2 and sampling line C, carry out sterilizing, when needs sample, steam control valve T2 closes, steam in steam-pipe can not enter intake ducting A, in sampling valve 2 and sampling line C, simultaneously, in the present embodiment, the size of steam flow in all right steam regulation pipeline B of steam control valve T2.
Further, in the present embodiment, the first end 2b of sampling valve 2 is connected by sterile connector with retort thief hole, the aseptic connection between sampling unit and thief hole is achieved by the connection of sterile connector, and in prior art, what the sampling valve interface in sampling unit was corresponding is standard chuck port, can debris be there is in sampling rear port place, first discharge opeing is needed before causing sampling, so often waste enchylema, in the present embodiment, the first end 2b of sampling valve 2 is connected with retort thief hole effectively can be prevented debris by sterile connector, first discharge opeing is carried out before not needing sampling.
Further, in the present embodiment, sampling line C arranges connection valve T3, and sampling line C is communicated with sampling jug 3, during sampling by connection valve T3, when connection valve T3 opens, sampling liquid then in sampling line C flows in sampling jug 3, and when connection valve T3 closes, then the sampling liquid in sampling line C cannot flow in sampling jug 3, in the present embodiment, sampling jug 3 can be entered to the sampling liquid in sampling line C control by sampling line C being arranged connection valve T3.
Further, in the present embodiment, sampling line C between connection valve T3 and the 3rd end 2c of sampling valve 2 connects blow-off line D, and blow-off line D is provided with wash water valve T4, in the present embodiment, during sampling, steam control valve T2, wash water valve T4 and intake valve T1 closes, connection valve T3 opens, the enchylema of sampling enters sampling jug 3 from sampling valve 2 through sampling line C, after sampling, intake valve T1 and wash water valve T4 opens, pressurized air is pressed in intake ducting A, enchylema residual in sampling valve 2 and sampling line C is discharged from blow-off line D or sampling line B, before lower sub-sampling, intake valve T1 and connection valve T3 closes, steam control valve T2 and wash water valve T4 opens, the steam of input is to intake ducting A, sampling valve 2 and sampling line C carry out sterilizing, after sterilizing, steam is discharged from blow-off line D, it should be noted that, in the present embodiment, air filter disc 1, each control valve and sampling jug 3 can pass through high-pressure sterilizing pot sterilizing before sterilizing, sampling valve is connected again after sterilizing.
Further, in the present embodiment, sampling line C and blow-off line D is plastic conduit, it can be such as PVC pipeline, such sampling line C and blow-off line D inwall smoother, during sampling, the resistance received among sampling line C from the liquid of thief hole outflow is less, and not easily keep on inner-walls of duct, be convenient to liquid and enter sampling jug 3, simultaneously, when being blown into pressurized air in intake ducting, liquid residual in sampling valve 2 and sampling line C, because of inner-walls of duct smoother, is also easy to discharge, thus greatly reduces ducted residual quantity.
Last it is noted that above each embodiment is only in order to illustrate the technical solution of the utility model, be not intended to limit; Although be described in detail the utility model with reference to foregoing embodiments, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein some or all of technical characteristic; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the scope of each embodiment technical scheme of the utility model.

Claims (7)

1. a sampling unit, is characterized in that, comprising:
Sampling valve, sampling jug, sampling line, intake ducting, air filter disc and steam-pipe;
Wherein, the first end of described sampling valve is connected with the thief hole of retort, and the second end of described sampling valve is connected with described sampling jug by described sampling line, and the 3rd end of described sampling valve is connected with described intake ducting;
Described air filter disc is arranged on described intake ducting, and described air filter disc to input compressed-air in described intake ducting, can be discharged to make the debris in described sampling valve and described sampling line;
Described steam-pipe is connected to the intake ducting between described air filter disc and described sampling valve, and in described steam-pipe, input can carry out the steam of sterilizing to described intake ducting, described sampling valve and described sampling line.
2. sampling unit according to claim 1, is characterized in that, described intake ducting is provided with intake valve, and on the intake ducting of described intake valve between described air filter disc and described steam-pipe.
3. sampling unit according to claim 1 and 2, is characterized in that, described steam-pipe is provided with steam control valve.
4. sampling unit according to claim 1 and 2, is characterized in that, the first end of described sampling valve is connected by sterile connector with described retort thief hole.
5. sampling unit according to claim 1 and 2, is characterized in that, described sampling line arranges connection valve, and described sampling line is communicated with described sampling jug by described connection valve.
6. sampling unit according to claim 5, is characterized in that, the sampling line between described connection valve and the 3rd end of described sampling valve connects blow-off line, and described blow-off line is provided with wash water valve.
7. sampling unit according to claim 6, is characterized in that, described sampling line and described blow-off line are plastic conduit.
CN201520475791.3U 2015-07-03 2015-07-03 Sample taking device Active CN204848870U (en)

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Application Number Priority Date Filing Date Title
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111879573A (en) * 2020-06-22 2020-11-03 安徽创华自控仪表设备有限公司 Pipeline sampling valve

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111879573A (en) * 2020-06-22 2020-11-03 安徽创华自控仪表设备有限公司 Pipeline sampling valve

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Address after: 101111 Room 202, building D, building 33, No. 99, kekechuang 14th Street, Beijing Economic and Technological Development Zone, Beijing

Patentee after: Zhongsheng tianxinhe (Wuxi) Biotechnology Co., Ltd

Address before: 101111 Room 202, building D, building 33, No. 99, kekechuang 14th Street, Beijing Economic and Technological Development Zone, Beijing

Patentee before: Beijing tianxinhe Biotechnology Co., Ltd