CN203700381U - Integrated device for microperfusion cell culture and collection of product thereof - Google Patents
Integrated device for microperfusion cell culture and collection of product thereof Download PDFInfo
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- CN203700381U CN203700381U CN201420055861.5U CN201420055861U CN203700381U CN 203700381 U CN203700381 U CN 203700381U CN 201420055861 U CN201420055861 U CN 201420055861U CN 203700381 U CN203700381 U CN 203700381U
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- perfusion
- culture
- nutrient solution
- water bath
- flow ipe
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Abstract
The utility model discloses an integrated device for microperfusion cell culture and collection of a product thereof. The integrated device comprises a culture gas chamber, a constant-temperature water bath, a constant-temperature system, perfusion grooves, a culture solution ultrafilter and a perfusion solution collection vessel, wherein the culture gas chamber, the constant-temperature water bath, the constant-temperature system and the perfusion grooves are arranged in a culture vessel, meanwhile, a culture gas inlet and a culture gas overflow hole are formed in the culture vessel; the lower parts of the perfusion grooves are positioned in the constant-temperature water bath and meanwhile are connected with the culture liquid ultrafilter via inflow pipelines; the upper parts of the perfusion grooves are located in the culture gas chamber and meanwhile are connected with the perfusion solution collection vessel via outflow pipelines. The integrated device integrates automatic liquid feeding, automatic gas feeding and automatic and continuous metabolite collecting, can avoid pollution and experiment loss, and can simplify operation process and improve the precision of the experimental result besides.
Description
Technical field
The utility model relates to a kind of micro perfusion cell cultures and product collection integrating device
Background technology:
While carrying out cell cultures, because needs interval certain hour is changed nutrient solution and removes metabolic waste, therefore may increase the systematic error of experiment.Especially while manually changing nutrient solution, not only make pollution probability increase, the liquid flow impact also may be because of application of sample time stirs cell, affect cell attachment, even also may occur cell suction or wash out and cause experiment loss.In addition, cell in vitro is cultivated the detection of metabolite at present, to adopt instant meta-bolites to detect, its detection method is by after cell cultures for some time, the nutrient solution extracting in culture vessel detects, and this detection method exists following shortcoming: (1) experimental result is likely subject to the interference of secondary metabolite and has error; (2) in order to collect meta-bolites and then Extending culture time, cause on the contrary metabolic waste to affect cellular metabolism type and affect experiment expection, even also may too much cause cellular metabolism abnormal or dead because of metabolite.Yet there are no the report of the device that integrates automatic liquid supply, automatic feeding, Automatic continuous collection meta-bolites.
Summary of the invention
The purpose of this utility model is to provide a kind of micro perfusion cell cultures and product collection integrating device, this device sustainable supply nutrient solution, and automatically collect meta-bolites.
For achieving the above object, the solution that the utility model adopts is: a kind of micro perfusion cell cultures and product collection integrating device, comprise cultivation gas compartment, water bath with thermostatic control, constant temperature system, perfusion groove, nutrient solution ultra-fine filter and perfusate collection container, described cultivation gas compartment, water bath with thermostatic control, constant temperature system and perfusion groove are located in culture vessel, wherein cultivate gas compartment and be positioned at the top of culture vessel, water bath with thermostatic control and constant temperature system lay respectively at middle part and the bottom of culture vessel, on culture vessel, be also provided with and cultivate gas input hole and cultivate gas overflowing hole simultaneously, the bottom of described perfusion groove is arranged in water bath with thermostatic control, and be connected with nutrient solution ultra-fine filter by its flow ipe, the top of perfusion groove is arranged in cultivates gas compartment, and be connected with perfusate collection container by its outflow pipeline.
The flow ipe of above-mentioned perfusion groove is or/and be also provided with doser on the flow ipe of nutrient solution ultra-fine filter.Described doser is valve-type doser, formed by Y-piece road, valve rack, nut and increased pressure board, wherein two ports in Y-piece road are connected on the flow ipe of perfusion groove or the flow ipe of nutrient solution ultra-fine filter, another port is sealed by rubber diaphragm, form dosing and thrust position, nut and increased pressure board are arranged on Y-piece road by valve rack, advance increased pressure board can reduce caliber by rotating nut.
Above-mentioned perfusion groove is multiple, and nutrient solution ultra-fine filter is connected with the flow ipe of each perfusion groove by perfusion skimmer, on the flow ipe of nutrient solution ultra-fine filter and each flow ipe of perfusion groove, is equipped with doser.
Above-mentioned perfusion groove is funnel-form, and nutrient solution influx place, its underpart is provided with buffering ball, and middle part is provided with buffering filtering membrane, and micropore glass Tissue Culture Plate is positioned on buffering filtering membrane.
Use procedure of the present utility model is: first will after the water bath with thermostatic control heating of system, maintain the optimum temps of cell cultures, then culture systems gas is passed into, and regulate airshed, open the microsyringe of micro perfusion nutrient solution, adjust flow by automatic fine syringe, continuous micro carries out nutrient solution supply, and makes liquid all enter perfusate collection container by miniature perfusion concentrated flow, makes nutrient solution be full of the whole tubing system of perfusion.Then adhesion being needed to the micropore glass Tissue Culture Plate of culture experiment cell is placed on the buffering filtering membrane in slight irrigation chute, and is submerged in nutrient solution, enters perfusion, notes aseptic technique.Meta-bolites as collected in need, the nutrient solution of collecting in selected Hemoperfusion time section.As need add observed drug metabolic effect, carry out dosing by perfusion valve doser, thrust rubber bag tank with pipettor tip and push, wherein give identical drug intervention to the cell of all slight irrigation chutes in this way, can carry out dosing by overall channel perfusion valve doser; Carry out different drug treating for different slight irrigation chute cells in this way, be chosen in single passage perfusion valve doser and inject corresponding medicine, now can settle multiple perfusate collection containers simultaneously, synchronously start perfusion, intervention, collection perfusate and analyze, can reduce the result that environment heterogeneity causes untrue.If coordinate with protein analysis system, in the continuous irrigation prognosis that drains off, collecting the product of different time sections analyzes, the Changing Pattern that after can showed cell a certain stimulus being stimulated, metabolism occurs in time, thus can be by the time sequence drugs of meta-bolites the rule that affects on cellular metabolism.
The utility model integrates automatic liquid supply, automatic feeding, Automatic continuous collection meta-bolites, and can give drug intervention the instant meta-bolites of collecting in different time points, thereby can avoid polluting and experiment loss, and can simplify the operation course and improve the precision of experimental result.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
Fig. 2 is the link schematic diagram of nutrient solution ultra-fine filter, skimmer, doser, perfusate collection container and multiple perfusion grooves.
Fig. 3 is the structural representation of perfusion groove.
Fig. 4 is the structural representation of nutrient solution ultra-fine filter.
Fig. 5 is the structural representation of valve-type doser.
In figure: 1-culture vessel 2-cultivation gas compartment, 3-constant temperature system, 4-water bath with thermostatic control, 5-nutrient solution ultra-fine filter, 6-perfusion groove, 7-perfusate collection container 8-cultivation gas input hole 9-cultivation gas overflowing hole, 10-perfusion skimmer, 11-buffering ball 12-buffering filtering membrane 13-micropore glass Tissue Culture Plate 14-ultra-filtration membrane, 15-doser, 16-doser, 17-Y-piece road, 18-nut, 19-increased pressure board, 20-valve rack, 21-rubber diaphragm
Embodiment
As shown in Figure 1, the utility model is mainly by culture vessel 1, cultivate gas compartment 2, constant temperature system 3, water bath with thermostatic control 4, nutrient solution ultra-fine filter 5, perfusion groove 6, perfusate collection container 7, perfusion skimmer 10 and doser 15, 16 compositions, the top of culture vessel 1 is the lid that there is sealed strip at edge, cultivate gas compartment 2, water bath with thermostatic control 4, constant temperature system 3 and miniature perfusion groove 6 are located in culture vessel 1, wherein cultivate gas compartment 2 and be positioned at the top of culture vessel 1, water bath with thermostatic control 4 and constant temperature system 3 lay respectively at middle part and the bottom of culture vessel 1, on the lid of culture vessel 1, be also provided with input CO simultaneously
2in input hole 8 and the cultivation gas overflowing hole 9 of cultivating gas, overfolw hole 9 is connected with gas recovery system.Miniature perfusion groove 6 is funnel-form, its tapered segment is arranged in water bath with thermostatic control 4, its cone tip portion is connected with nutrient solution ultra-fine filter 5 with adaptive plug, joint by flow ipe, column part is arranged in cultivates gas compartment 2, and is connected with perfusate collection container 7 with adaptive plug, joint by its outflow pipeline.
Perfusion groove 6 can adopt one or more, when perfusion groove 6 is while being multiple, as shown in Figure 2, nutrient solution ultra-fine filter 5 is connected by perfusion skimmer 10 and each perfusion groove 6, now perfusate collection container 7 as required, can be one, also can connect a perfusate collection container 7 by each perfusion groove 6.As can be seen from Figure 3, cone point opening part at perfusion groove 6 is also provided with a buffering ball 11, be provided with semi-transparent buffering filtering membrane 12 at circular cone and column part intersection, on buffering filtering membrane 12, place micropore glass Tissue Culture Plate 13, buffering ball 11 can prevent that perfusate is counter and flow, and can cushion perfusate and impact cell cultures sheet glass 13, buffering filtering membrane 12 can reduce perfusion liquid-flow the impact of cell is come off.
Nutrient solution ultra-fine filter 5 as shown in Figure 4, is UFO-like, which is provided with liquid influx, is wherein stained with ultra-filtration membrane 14, for filtering the impurity such as bacterium, and its lower perfusion skimmer 10 that connects.
The utility model is also provided with valve-type doser 15 and 16 on the flow ipe of nutrient solution ultra-fine filter 5 and the flow ipe of each perfusion groove 6, described valve-type doser 15 and 16 is by Y-piece road 17, valve rack 20, nut 18 and increased pressure board 19 form, wherein two ports in Y-piece road 17 are connected on the flow ipe of perfusion groove 6 or the flow ipe of nutrient solution ultra-fine filter 5, another port is sealed by rubber diaphragm 21, when dosing, rifle head thrusts from rubber diaphragm 21, dosing is complete extracts after rifle head, puncturing hole can be closed voluntarily, thereby can prevent that liquid is counter flows, form dosing and thrust position.Nut 18 and increased pressure board 19 are arranged on Y-piece road 17 by valve rack 20, and rotating nut 18 can advance increased pressure board 19, thereby can reduce caliber until close, to realize flow control and switch.
The foregoing is only one of the utility model preferred embodiment, it is pointed out that those of ordinary skills not departing under the prerequisite of the utility model use principle, can also do some modification and improvement, also should be considered as the utility model protection domain.
Claims (6)
1. a micro perfusion cell cultures and product collection integrating device, comprise and cultivate gas compartment (2), constant temperature system (3), water bath with thermostatic control (4), nutrient solution ultra-fine filter (5), perfusion groove (6) and perfusate collection container (7), it is characterized in that: described cultivation gas compartment (2), constant temperature system (3), water bath with thermostatic control (4) and perfusion groove (6) are located in culture vessel (1), wherein cultivate gas compartment (2) and be positioned at the top of culture vessel (1), water bath with thermostatic control (4) and constant temperature system (3) lay respectively at middle part and the bottom of culture vessel (1), on culture vessel (1), be also provided with and cultivate gas input hole (8) and cultivate gas overflowing hole (9) simultaneously, the bottom of described perfusion groove (6) is arranged in water bath with thermostatic control (4), and be connected with nutrient solution ultra-fine filter (5) by its flow ipe, the top of perfusion groove (6) is arranged in cultivates gas compartment (1), and be connected with perfusate collection container (7) by its outflow pipeline.
2. a kind of micro perfusion cell cultures according to claim 1 and product collection integrating device, is characterized in that: at the flow ipe of perfusion groove (6) or/and be also provided with doser (15,16) on the flow ipe of nutrient solution ultra-fine filter (5).
3. a kind of micro perfusion cell cultures according to claim 2 and product collection integrating device, it is characterized in that: described doser (15, 16) be valve-type doser, by Y-piece road (17), nut (18), increased pressure board (19) and valve rack (20) form, wherein two ports in Y-piece road (17) are connected on the flow ipe of perfusion groove (6) or the flow ipe of nutrient solution ultra-fine filter (5), another port is sealed by rubber diaphragm (21), form dosing and thrust position, nut (18) and increased pressure board (19) are arranged on Y-piece road (17) by valve rack (20), advance increased pressure board (19) can reduce caliber by rotating nut (18).
4. a kind of micro perfusion cell cultures according to claim 1 and product collection integrating device, it is characterized in that: described perfusion groove (6) is for multiple, and nutrient solution ultra-fine filter (5) is connected with the flow ipe of each perfusion groove (6) by perfusion skimmer (10).
5. a kind of micro perfusion cell cultures according to claim 4 and product collection integrating device, is characterized in that: on the flow ipe of nutrient solution ultra-fine filter (5) and each flow ipe of perfusion groove (6), be equipped with doser.
6. a kind of micro perfusion cell cultures according to claim 1 and product collection integrating device, it is characterized in that: perfusion groove (6) is funnel-form, nutrient solution influx place, its underpart is provided with buffering ball (11), middle part is provided with buffering filtering membrane (12), and micropore glass Tissue Culture Plate (13) is positioned on buffering filtering membrane (12).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420055861.5U CN203700381U (en) | 2014-01-28 | 2014-01-28 | Integrated device for microperfusion cell culture and collection of product thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420055861.5U CN203700381U (en) | 2014-01-28 | 2014-01-28 | Integrated device for microperfusion cell culture and collection of product thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203700381U true CN203700381U (en) | 2014-07-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420055861.5U Expired - Fee Related CN203700381U (en) | 2014-01-28 | 2014-01-28 | Integrated device for microperfusion cell culture and collection of product thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203700381U (en) |
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2014
- 2014-01-28 CN CN201420055861.5U patent/CN203700381U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140709 Termination date: 20150128 |
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| EXPY | Termination of patent right or utility model |