CN204824906U - Specific nucleic acid separation, enrichment reaction unit - Google Patents
Specific nucleic acid separation, enrichment reaction unit Download PDFInfo
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- CN204824906U CN204824906U CN201520550310.0U CN201520550310U CN204824906U CN 204824906 U CN204824906 U CN 204824906U CN 201520550310 U CN201520550310 U CN 201520550310U CN 204824906 U CN204824906 U CN 204824906U
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- nucleic acid
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Abstract
The utility model discloses a specific nucleic acid separation, enrichment reaction unit, it includes the mouth of pipe, centrifuging tube body, first pipeline, bottle plug and second pipeline, and first pipeline is straight shape, and the second pipeline is the form of buckling, and the bottle plug is connected with the one end of first pipeline, the centre and the second pipe connection of first pipeline, the other end of first pipeline is connected on the centrifuging tube body, and the mouth of pipe is on the top of centrifuging tube body. The device can separate total nucleic acid as the nucleic acid isolation system, also can regard as specificity enrichment nucleic acid isolation system, treats the gene of research and does the separation, simultaneously, enrichment or separation be behind the nucleic acid, this reaction tube can be directly as the PCR reaction tube and do not influence reverse transcription, PCR, RT -PCR reaction. When nucleic acid extraction handles, do not need the adsorption column, can adsorb enrichment nucleic acid through the specificity primer of internal surface to reach the mesh that improves nucleic acid extraction and rate.
Description
Technical field
The utility model relates to the separation of a kind of specific nucleic acid, enrichment reaction unit, and after particularly relating to a kind of enrichment or isolating nucleic acid, this reaction tubes can directly as PCR(PolymeraseChainReaction, polymerase chain reaction) reaction tubes.
Background technology
Existingly to be separated total nucleic acid, process etc. needs multiple reaction unit and adsorption column, and such nucleic acid extraction rate is low, and cost is high.
Summary of the invention
Technical problem to be solved in the utility model is to provide the separation of a kind of specific nucleic acid, enrichment reaction unit, it can be separated total nucleic acid as separate nucleic acid system, also as specific enrichment separate nucleic acid system, gene to be studied can be separated; Meanwhile, after enrichment or isolating nucleic acid, this reaction tubes directly can not affect reverse transcription, PCR, RT-PCR reaction as PCR reaction tubes.When nucleic acid extraction process, do not need adsorption column, namely by the Auele Specific Primer adsorption and enrichment nucleic acid of internal surface, thus reach the object improving nucleic acid extraction rate.
The utility model solves above-mentioned technical problem by following technical proposals: a kind of specific nucleic acid is separated, enrichment reaction unit, it is characterized in that, it comprises the mouth of pipe, centrifuge tube body, the first pipeline, bottle stopper and second pipe, first pipeline is straight shape, second pipe is bending, and bottle stopper is connected with one end of the first pipeline, and the centre of the first pipeline is connected with second pipe, the other end of the first pipeline is connected on centrifuge tube body, and the mouth of pipe is on the top of centrifuge tube body.
Preferably, described bottle stopper is provided with one and buckles, and bottle stopper surface is in screw thread form.
Preferentially, be provided with cock cap, Nei Sai and plastic layer in described first pipeline, interior plug is between cock cap and plastic layer.
Preferably, described second pipe is provided with an air outlet, and air outlet is above second pipe.
Preferably, described centrifuge tube body is provided with insulation film, and insulation film is on the inwall of centrifuge tube body.
Preferably, the bottom of described centrifuge tube body is provided with frosted layer.
Positive progressive effect of the present utility model is: the utility model can be separated total nucleic acid as separate nucleic acid system, also as specific enrichment separate nucleic acid system, can be separated gene to be studied; Meanwhile, after enrichment or isolating nucleic acid, this reaction tubes directly can not affect reverse transcription, PCR, RT-PCR reaction as PCR reaction tubes.When nucleic acid extraction process, do not need adsorption column, namely by the Auele Specific Primer adsorption and enrichment nucleic acid of internal surface, thus reach the object improving nucleic acid extraction rate.
Accompanying drawing explanation
Fig. 1 is the PCR pipe structural representation of the utility model tapered bottom internal surface frosted.
In figure: the mouth of pipe 1, centrifuge tube body 2, insulation film 3, air outlet 4, cock cap 5, the first pipeline 6, bottle stopper 7, buckles 8, interior plug 9, plastic layer 10, second mouth of pipe 11, frosted layer 12.
Embodiment
The utility model preferred embodiment is provided, to describe the technical solution of the utility model in detail below in conjunction with accompanying drawing.
As shown in Figure 1, the utility model specific nucleic acid is separated, enrichment reaction unit comprises the mouth of pipe 1, centrifuge tube body 2, first pipeline 6, bottle stopper 7 and second pipe 11, first pipeline 6 is in straight shape, second pipe 11 is in bending, bottle stopper 7 is connected with one end of the first pipeline 6, the centre of the first pipeline 6 is connected with second pipe 11, and the other end of the first pipeline 6 is connected on centrifuge tube body 2, and the mouth of pipe 1 is on the top of centrifuge tube body 2.
Bottle stopper 7 is provided with one and buckles 8, buckles contribute to depositing reaction tubes in not used time; Bottle stopper 7 surface in screw thread form, can turn on and avoid when getting liquid because improper operation makes overflow simultaneously by fast rotary during use.
Be provided with cock cap 5, interior plug 9 and plastic layer 10 in first pipeline 6, interior plug 9 is between cock cap 5 and plastic layer 10, and plastic layer completely cuts off the air entered because of the incomplete heat retaining property of possibility of bottle stopper 7, strengthens a bottle inside holding constant temperature effect, makes reaction more consummate.Cock cap 5 prevents liquid from not having complete bottle inlet and overflowing.
Second pipe 11 is provided with an air outlet 4, and air outlet 4 is above second pipe 11, and when operating, plastic layer 10 shrinks cock cap 5 and tightly forced up by interior plug 9, thus reaches the effect of air outlet 4 exhaust.
Centrifuge tube body 2 is provided with insulation film 3, and insulation film 3 is on the inwall of centrifuge tube body 2, and what prevent heat disperses effective insulation.
The bottom of described centrifuge tube body is provided with frosted layer 12, increases its surface-area.
The utility model can be separated total nucleic acid as separate nucleic acid system, is separated, also as specific enrichment separate nucleic acid system, can be separated gene to be studied particular by centrifuge tube body, the first pipeline, second pipe etc.; Meanwhile, after enrichment or isolating nucleic acid, this reaction tubes directly can not affect reverse transcription, PCR, RT-PCR reaction as PCR reaction tubes.When nucleic acid extraction process, do not need adsorption column, namely by the Auele Specific Primer adsorption and enrichment nucleic acid of internal surface, thus reach the object improving nucleic acid extraction rate.
Described the utility model specific nucleic acid is separated, enrichment reaction unit, it is when nucleic acid extraction process, do not need adsorption column, namely by the Auele Specific Primer adsorption and enrichment nucleic acid of internal surface, thus reaching the object improving nucleic acid extraction rate, when being separated object nucleic acid in multiple nucleic acids mixture, object nucleic acid is separated from total nucleic acid by the effect be combined with the specific primer specificity of internal surface, thus obtain the higher object nucleic acid of purity, be conducive to subsequent experimental operation.Due to the specificity of its internal surface modifier, after enrichment, isolating nucleic acid, this system directly as PCR reaction tubes, and can not affect PCR, RT-PCR reaction.
Internal surface at the bottom of centrifuge tube is after frosted process, increase its surface-area, can maximize and improve nucleic acid and the specific binding reaction efficiency modifying probe, 5 '-(SH)-(T) 25-3 ' also can substitute by specificity synthetic oligonucleotide fragment, for adsorbing specific DNA, RNA, carry out separate nucleic acid.The reaction tubes of frosted process in pipe, matte surface marked the oligonucleotide of Immobilizer amino, 5 ' marking sulfhydryl, and under given conditions, sulfydryl reacts, by oligonucleotide marker at reaction tubes matte surface with amino.Wherein, centrifuge tube is PP material, specification has 1.5mL, 0.5mL, 0.2mL and 0.1mL, the primer 5 ' modified in centrifuge tube tapered bottom frosted inner surface holds connection-SH, can with nucleic acid covalent attachment, as in conjunction with 5 '-(SH)-(T) 25-3 ', for 3 ' poly(A) mRNA specific adsorption and reverse transcription.
Above-described specific embodiment; the technical problem of solution of the present utility model, technical scheme and beneficial effect are further described; be understood that; the foregoing is only specific embodiment of the utility model; be not limited to the utility model; all within spirit of the present utility model and principle, any amendment made, equivalent replacement, improvement etc., all should be included within protection domain of the present utility model.
Claims (6)
1. a specific nucleic acid be separated, enrichment reaction unit, it is characterized in that, it comprises the mouth of pipe, centrifuge tube body, the first pipeline, bottle stopper and second pipe, first pipeline is straight shape, second pipe is bending, and bottle stopper is connected with one end of the first pipeline, and the centre of the first pipeline is connected with second pipe, the other end of the first pipeline is connected on centrifuge tube body, and the mouth of pipe is on the top of centrifuge tube body.
2. specific nucleic acid separation as claimed in claim 1, enrichment reaction unit, it is characterized in that, described bottle stopper is provided with one and buckles, and bottle stopper surface is in screw thread form.
3. specific nucleic acid separation as claimed in claim 1, enrichment reaction unit, it is characterized in that, be provided with cock cap, Nei Sai and plastic layer in described first pipeline, interior plug is between cock cap and plastic layer.
4. specific nucleic acid separation as claimed in claim 1, enrichment reaction unit, it is characterized in that, described second pipe is provided with an air outlet, and air outlet is above second pipe.
5. specific nucleic acid separation as claimed in claim 1, enrichment reaction unit, it is characterized in that, described centrifuge tube body is provided with insulation film, and insulation film is on the inwall of centrifuge tube body.
6. specific nucleic acid separation as claimed in claim 1, enrichment reaction unit, it is characterized in that, the bottom of described centrifuge tube body is provided with frosted layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520550310.0U CN204824906U (en) | 2015-07-28 | 2015-07-28 | Specific nucleic acid separation, enrichment reaction unit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520550310.0U CN204824906U (en) | 2015-07-28 | 2015-07-28 | Specific nucleic acid separation, enrichment reaction unit |
Publications (1)
| Publication Number | Publication Date |
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| CN204824906U true CN204824906U (en) | 2015-12-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201520550310.0U Active CN204824906U (en) | 2015-07-28 | 2015-07-28 | Specific nucleic acid separation, enrichment reaction unit |
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| CN (1) | CN204824906U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116510994A (en) * | 2023-07-03 | 2023-08-01 | 中国农业大学 | PCR tube for integrally extracting genome |
-
2015
- 2015-07-28 CN CN201520550310.0U patent/CN204824906U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116510994A (en) * | 2023-07-03 | 2023-08-01 | 中国农业大学 | PCR tube for integrally extracting genome |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: Specific nucleic acid separation, enrichment reaction unit Effective date of registration: 20180914 Granted publication date: 20151202 Pledgee: Beijing Leadman Biochemistry Co., Ltd. Pledgor: Ke Yi bio tech ltd, Shanghai Registration number: 2018310000060 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20200103 Granted publication date: 20151202 Pledgee: Beijing Leadman Biochemistry Co., Ltd. Pledgor: Ke Yi bio tech ltd, Shanghai Registration number: 2018310000060 |