CN204051713U - Micropipettor - Google Patents

Micropipettor Download PDF

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Publication number
CN204051713U
CN204051713U CN201420485623.8U CN201420485623U CN204051713U CN 204051713 U CN204051713 U CN 204051713U CN 201420485623 U CN201420485623 U CN 201420485623U CN 204051713 U CN204051713 U CN 204051713U
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China
Prior art keywords
capillary
core pipe
inner chamber
micropipettor
cranse
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CN201420485623.8U
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Chinese (zh)
Inventor
梅占龙
吴靓
李阳
李华昕
林欣欣
陈晓红
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BGI Shenzhen Co Ltd
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BGI Shenzhen Co Ltd
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Abstract

The utility model belongs to laboratory equipment technical field, provide a kind of micropipettor, it comprises: there is the shell of inner chamber, core pipe that inner chamber is located in activity, for the capillary of imbitition and for providing the pressurizer of air pressure for inner chamber, the upper end that shell upper end is provided with Rong Xin pipe is stretched out and close-fitting movable span with it, its lower end is provided with the outlet spout holding capillary and pass, and the outer wall of outlet spout and capillary closely cooperates; Core pipe has the container cavity for holding capillary, can push out the push part of capillary when its lower end is provided with pressing core pipe upper end to outlet spout, and the tube wall of core pipe is provided with the through hole that the air holding inner chamber enters container cavity; Pressurizer is located on shell.The utility model adopts capillary to utilize capillarity imbitition, improves air pressure inside and extrudes liquid, can effectively prevent liquid contaminated by pressurizer; Capillary in core pipe can be pushed to outside by its push part, can form new capillary port after cutting off used part.

Description

Micropipettor
Technical field
The utility model belongs to laboratory equipment technical field, particularly relates to a kind of micropipettor.
Background technology
At biochemical field, often need the transfer carrying out micro liquid, the transfer of such as micro liquid between ultramicron reactor.In order to the requirement of satisfied transfer micro liquid, in prior art, devised lot of trace pipettor, but these micropipettors still cannot meet the requirement of many ultramicron reactors (such as the biochip in 5184 holes).The aperture of 5184 hole biochips is 470 μm, the liquid of 100-200nL can be held in hole, and the range of existing micropipettor is generally at 0.1 μ L-1mL, the bore of its imbitition, much larger than 470 μm, cannot carry out absorption transfer to the liquid in above-mentioned 5184 hole biochips.In addition, product in biochip is nucleic acid or protein matter often, it has quite high requirement to the clean level of pipettor, therefore after pipettor often shifts a liquid, the fluid suction head (or claiming rifle head) just needing replacing one new, change with high costs, the operation inconvenience of fluid suction head, cause the cost of whole pipetting also to improve thereupon.Meanwhile, also have that artificial to understand breach footpath little and change the high problem of fluid suction head cost, adopt capillary imbitition, but they adopt is that liquid is blown out capillary by the mode that mouth blows, this mode obviously easy contaminated liquid, causes liquid dead.
Utility model content
The purpose of this utility model is the defect overcoming prior art, provide a kind of operate simple and easy, with low cost, can the micropipettor of preventing pollution liquid.
The utility model realizes like this, a kind of micropipettor, it comprises: there is the shell of inner chamber, core pipe that described inner chamber is located in activity, for the capillary of imbitition and for providing the pressurizer of air pressure for described inner chamber, described shell upper end is provided with the upper end holding described core pipe and stretches out and close-fitting movable span with it, its lower end is provided with the outlet spout holding described capillary and pass, and the outer wall of described outlet spout and described capillary closely cooperates; Described core pipe has the container cavity for holding described capillary, its lower end can push out the push part of described capillary to described outlet spout when being provided with the described core pipe upper end of pressing, the tube wall of described core pipe is provided with the through hole that the air holding described inner chamber enters described container cavity; Described pressurizer is located on described shell.
Further, described push part comprise several be located at described core pipe lower end for clamp described capillary and can outwards elasticity launch clamping lobe, the thickness of described clamping lobe diminishes from the bottom to top gradually, the root of each described clamping lobe and the outer wall of described core pipe form step, clamp lobe and be enclosed to form clamping mouth for clamping described capillary described in several; Described push part also comprises the first spring and the cranse for tightening described clamping mouth, described first spring and described cranse are all sheathed on the outer wall of described clamping mouth, the internal diameter of described cranse is less than the maximum outside diameter of described clamping mouth, and the two ends of described first spring support described step and described cranse respectively; Described inner chamber lower end is provided with the counterbore holding described clamping mouth and stretch into, and the aperture of described counterbore is greater than external diameter when described clamping mouth launches and is less than the external diameter of described cranse, and described cranse supports the bottom of described inner chamber.
Further, described cranse is C type circle.
Preferably, described push part comprises two or three described clamping lobes.
Particularly, the end outer wall of each described clamping lobe is equipped with the projection for preventing described cranse slippage.
Further, described pressurizer comprises sleeve, the activity with movable chamber and is located at piston in described movable chamber and pull bar, described sleeve upper end is provided with the push-and-pull hole holding described pull bar and pass, and described pull bar upper end is provided with pressure cap, and its lower end is connected with described piston; Described pressurizer also comprises the second spring, and described second spring housing is located on described pull bar, and its two ends support described pressure cap and described sleeve respectively; The lower end of described sleeve is connected with the outer wall of described shell, described shell is provided with the passage being communicated with described inner chamber and described movable chamber, be provided with in described passage and hold the first valve that described movable chamber air enters described inner chamber, described sleeve is provided with air admission hole, is provided with and holds the second valve that outside air enters described movable chamber in described air admission hole.
Further, described air admission hole peripheral hardware has the screen pack for filtered air.
Particularly, the upper end of described core pipe is provided with and enters the mouth of pipe for what add capillary in described container cavity, described in enter on the mouth of pipe to be covered with pipe lid.
More specifically, the upper end of described core pipe is arranged with soft seal cover, and the upper end outer wall of described pipe lid and described shell is supported at described soft seal cover two ends respectively.
Further, described shell comprises body portion section and head sections, and described head sections and described body portion section are tightly connected.
Micropipettor provided by the utility model has following technique effect:
The utility model adopts capillary to utilize capillarity to draw micro liquid, after micropipettor being transferred to target place, the air pressure of inner chamber is improved by pressurizer, air pressure in indirect raising capillary, thus the liquid extruded in capillary, finally reach the object drawn and discharge micro liquid, omnidistance without the need to manually blowing out the liquid in capillary, can effectively prevent liquid contaminated.In addition, the utility model is also by arranging push part, the capillary be installed in core pipe can be pushed to outside, after cutting off that a part of capillary being infected with liquid, new clean free of contamination capillary port can be formed, thus simplify the program changing capillary, saved the running cost moving liquid simultaneously.
Accompanying drawing explanation
Fig. 1 is the longitudinal sectional view of micropipettor in the utility model embodiment;
Fig. 2 is the partial enlarged drawing of I in Fig. 1;
Fig. 3 is the partial enlarged drawing of II in Fig. 1.
Detailed description of the invention
In order to make the purpose of this utility model, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the utility model is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the utility model, and be not used in restriction the utility model.
See Fig. 1-Fig. 3, the utility model embodiment provides a kind of micropipettor, it comprises: there is the shell 1 of inner chamber 11, core pipe 2 that described inner chamber 11 is located in activity, for the capillary 3 of imbitition and for providing the pressurizer 4 of air pressure for described inner chamber 11, described shell 1 upper end is provided with the upper end holding described core pipe 2 and stretches out and close-fitting movable span 12 with it, its lower end is provided with the outlet spout 13 holding described capillary 3 and pass, and described outlet spout 13 closely cooperates with the outer wall of described capillary 3; Described core pipe 2 has the container cavity 21 for holding described capillary 3, its lower end can push out the push part 22 of described capillary 3 to described outlet spout 13 when being provided with pressing described core pipe 2 upper end, the tube wall of described core pipe 2 is provided with the through hole 23 that the air holding described inner chamber 11 enters described container cavity 21; Described pressurizer 4 is located on described shell 1.
Capillary 3 lower port of above-mentioned micropipettor immersed in liquid, capillary 3 sucks micro liquid automatically due to capillarity, now can complete the minim suction of liquid; Then micropipettor is moved, its capillary 3 lower port is placed on target location, control pressurizer 4 and provide micro-air pressure to inner chamber 11, air pressure flows through container cavity 21 by through hole 23 and imports in capillary 3, because capillary 3 internal gas pressure trace promotes, the micro liquid of its inside is extruded, the transfer of micro liquid can be completed.After often having shifted a liquid, the upper end of pressing core pipe 2, makes push part 22 push out the capillary 3 of certain length, is cut off by that a part of capillary being infected with liquid, new clean free of contamination capillary 3 lower port can be formed, carry out the pipetting of next round.
The utility model adopts capillary 3 to utilize capillarity to draw micro liquid, after micropipettor being transferred to target place, the air pressure of inner chamber 11 is improved by pressurizer 4, air pressure in indirect raising capillary 3, thus the liquid extruded in capillary, finally reach the object drawn and discharge micro liquid, omnidistance without the need to manually blowing out the liquid in capillary 3, can effectively prevent (or reduction) liquid contaminated.In addition, the utility model is also by arranging push part 22, the capillary 3 be installed in core pipe 2 can be pushed to outside, after cutting off that a part of capillary being infected with liquid, new clean free of contamination capillary port can be formed, thus simplify the program changing capillary 3, saved the running cost moving liquid simultaneously.
Specifically see Fig. 3, described push part 22 comprise several be located at described core pipe 2 lower end for clamp described capillary 3 and can outwards elasticity launch clamping lobe 221, the thickness of described clamping lobe 221 diminishes from the bottom to top gradually, the root of each described clamping lobe 221 and the outer wall of described core pipe 2 form step 24, clamp lobe 221 and be enclosed to form clamping mouth 222 for clamping described capillary 3 described in several; Described push part 22 also comprises the first spring 223 and the cranse 224 for tightening described clamping mouth 222, described first spring 223 and described cranse 224 are all sheathed on the outer wall of described clamping mouth 222, the internal diameter of described cranse 224 is less than the maximum outside diameter of described clamping mouth 222, and the two ends of described first spring 223 support described step 24 and described cranse 224 respectively; Described inner chamber 11 lower end is provided with the counterbore 111 holding described clamping mouth 222 and stretch into, and the aperture of described counterbore 111 is greater than external diameter when described clamping mouth 222 launches and is less than the external diameter of described cranse 224, and described cranse 224 supports the bottom of described inner chamber 11.
When pressing the upper end of core pipe 2, core pipe 2 moves down, clamping lobe 221 also moves down thereupon and enters in counterbore 111, cranse 224 is then by outside the counterbore 111 refused, the end of clamping lobe 221 (clamping mouth 222) is broken away from " bundle is fought " of cranse 224 and outwards launches, capillary 3 also breaks away from the clamping of clamping mouth 222 thereupon, due to gravity, stretches out downwards; When unclasping the upper end of core pipe 2, core pipe 2 oppositely movement, cranse 224 tightens clamping lobe 221 (clamping mouth 222) again, again firmly clamps capillary 3, now can cut off that a part of capillary 3 being infected with liquid.Cranse 224 in the utility model, except play tighten clamping mouth 222 effect except, also play the effect of sealing, prevent the circulation having air between inner chamber 11 and outlet spout 13.Can load many capillaries 3 in the container cavity 21 of core pipe 2, after a capillary 3 is finished, and then another capillary can stretch out outside outlet spout 13.
For the ease of assembling cranse 224, described cranse 224 can be C type circle, and the cranse 224 of C type can be buckled on clamping mouth 224 from the side.
Preferably, described push part 22 comprises two or three described clamping lobes 221, and test proves, two or three described clamping lobes 221 not only can clamp capillary 3, and its excellent, and the manufacture being also convenient to them is shaping.
Particularly, the end outer wall of each described clamping lobe 221 is equipped with the projection 2211 for preventing the slippage of described cranse 224, and protruding 2211 can strengthen the external diameter clamping mouth 222, prevent cranse 224 from departing from.
Specifically see Fig. 2, described pressurizer 4 comprises sleeve 41, the activity with movable chamber 411 and is located at piston 42 in described movable chamber 411 and pull bar 43, described sleeve 41 upper end is provided with the push-and-pull hole 412 holding described pull bar 43 and pass, described pull bar 43 upper end is provided with pressure cap 431, and its lower end is connected with described piston 42; Described pressurizer 4 also comprises the second spring 44, and described second spring 44 is sheathed on described pull bar 43, and its two ends support described pressure cap 431 and described sleeve 41 respectively; The lower end of described sleeve 41 is connected with the outer wall of described shell 1, described shell 1 is provided with the passage 14 being communicated with described inner chamber 11 and described movable chamber 411, be provided with air in the described movable chamber 411 of appearance in described passage 14 and enter the first valve 45 of described inner chamber 11, described sleeve 41 is provided with air admission hole 413, is provided with and holds the second valve 46 that outside air enters described movable chamber 411 in described air admission hole 413.
During pressing pressure cap 431, piston 42 slippage downwards, movable chamber 411 internal gas pressure raises, second valve 46 is closed, and the first valve 45 is opened, and the air in movable chamber 411 flows into inner chamber 11, air flows in container cavity 21 through through hole 23 again, and the liquid the most at last in capillary 3 is extruded.When unclasping pressure cap 431, due to the second spring 44 upwards pushing cap 431, movable chamber 411 internal gas pressure reduces, first valve 45 is closed, second valve 46 is opened, outside air, in air admission hole 413 inflow activity chamber 411, completes the inflation in movable chamber 411, for extruding liquid carrying for enough air next time.
As a kind of preferred embodiment of the present utility model, described air admission hole 413 peripheral hardware has the screen pack 47 for filtered air, and air is effectively filtered through screen pack 47, reduces extraneous air to the pollution of pipettor inside.
The upper end of described core pipe 2 is provided with and enters the mouth of pipe 25 for what add capillary 3 in described container cavity 21, described in enter on the mouth of pipe 25 to be covered with pipe lid 5.Capillary 3 cuts into the capillary tube segment of suitable length, and many capillaries Duan Kecong enters the mouth of pipe 25 and loads in container cavity 21.Certainly, capillary 3 can also insert in container cavity 21 from outlet spout 13, and prerequisite is inserting the upper end (i.e. pipe lid 5) needing always to press core pipe 2 in capillary 3 process.
The upper end of described core pipe 2 is arranged with soft seal cover 6, and the upper end outer wall that 6 two ends support described pipe lid 5 and described shell 1 is respectively overlapped in described soft seal.The gap between 6 salable pipe lids 5 and shell 1 upper end is overlapped in soft seal, prevents inside and outside gas channelling.
The conveniently manufacture of shell 1 and assembling, described shell 1 can comprise body portion section 15 and head sections 16, and described head sections 16 is tightly connected with described body portion section 15.Can be one-body molded between head sections 16 and body portion section 15, also can screw thread form engage.In order to prevent core pipe 2 from skidding off disengaging shell 1 by movable span 12, the ring wall of core pipe 2 is also provided with flange 26, and flange 26 is positioned at inner chamber 11, supports with the inwall of inner chamber 11.
Below in conjunction with above-mentioned concrete structure, the micropipettor working method that the utility model embodiment provides is described:
Micropipettor first loads capillary 3 before using, and makes its front end expose outlet spout 13 certain length;
The one end of capillary 3 being exposed outlet spout 13 is immersed in liquid, and capillary 3 utilizes capillarity (capillarity) automatically to suck micro liquid, completes the absorption of liquid;
Transfer micropipettor, capillary 3 is inhaled and has one end of liquid to aim at object container or target object, the pressure cap 431 of pressing pressurizer 4, gas in piston 42 Compressive activities chamber 411, makes it pressure and raises, push the first valve 45 (now the second valve 46 is in closed condition) open, air in movable chamber 411 flows into inner chamber 11 through passage 14, flow into container cavity 21 by inner chamber 11 via through holes 23 again, finally flow in capillary 3, liquid is extruded capillary 3;
Then pressure cap 431 is unclasped, due to the effect of the second spring 44, pressure cap 431 forced back towards original position, piston 42 also moves up thereupon and returns to original position, air pressure in movable chamber 411 declines thereupon, and the first valve 45 is closed, and the second valve 46 is opened, extraneous air is inhaled in movable chamber 411, and the air pressure in movable chamber 411 returns to original air pressure;
Pressing tube lid 5, core pipe 2 moves down thereupon, and push part 22 pushes out the capillary 3 of suitable length to outlet spout 13, and the part being infected with liquid is wiped out by operating personnel, forms new clean free of contamination capillary;
Circulation above-mentioned steps, can complete the operation of micro liquid transfer repeatedly.
The foregoing is only preferred embodiment of the present utility model; not in order to limit the utility model; all do within spirit of the present utility model and principle any amendment, equivalent to replace or improvement etc., all should be included within protection domain of the present utility model.

Claims (10)

1. a micropipettor, it is characterized in that, comprise: there is the shell of inner chamber, core pipe that described inner chamber is located in activity, for the capillary of imbitition and for providing the pressurizer of air pressure for described inner chamber, described shell upper end is provided with the upper end holding described core pipe and stretches out and close-fitting movable span with it, its lower end is provided with the outlet spout holding described capillary and pass, and the outer wall of described outlet spout and described capillary closely cooperates; Described core pipe has the container cavity for holding described capillary, its lower end can push out the push part of described capillary to described outlet spout when being provided with the described core pipe upper end of pressing, the tube wall of described core pipe is provided with the through hole that the air holding described inner chamber enters described container cavity; Described pressurizer is located on described shell.
2. micropipettor as claimed in claim 1, it is characterized in that: described push part comprise several be located at described core pipe lower end for clamp described capillary and can outwards elasticity launch clamping lobe, the thickness of described clamping lobe diminishes from the bottom to top gradually, the root of each described clamping lobe and the outer wall of described core pipe form step, clamp lobe and be enclosed to form clamping mouth for clamping described capillary described in several; Described push part also comprises the first spring and the cranse for tightening described clamping mouth, described first spring and described cranse are all sheathed on the outer wall of described clamping mouth, the internal diameter of described cranse is less than the maximum outside diameter of described clamping mouth, and the two ends of described first spring support described step and described cranse respectively; Described inner chamber lower end is provided with the counterbore holding described clamping mouth and stretch into, and the aperture of described counterbore is greater than external diameter when described clamping mouth launches and is less than the external diameter of described cranse, and described cranse supports the bottom of described inner chamber.
3. micropipettor as claimed in claim 2, is characterized in that: described cranse is C type circle.
4. micropipettor as claimed in claim 2, is characterized in that: described push part comprises two or three described clamping lobes.
5. micropipettor as claimed in claim 2, is characterized in that: the end outer wall of each described clamping lobe is equipped with the projection for preventing described cranse slippage.
6. the micropipettor as described in any one of claim 1-5, it is characterized in that: described pressurizer comprises sleeve, the activity with movable chamber and is located at piston in described movable chamber and pull bar, described sleeve upper end is provided with the push-and-pull hole holding described pull bar and pass, described pull bar upper end is provided with pressure cap, and its lower end is connected with described piston; Described pressurizer also comprises the second spring, and described second spring housing is located on described pull bar, and its two ends support described pressure cap and described sleeve respectively; The lower end of described sleeve is connected with the outer wall of described shell, described shell is provided with the passage being communicated with described inner chamber and described movable chamber, be provided with in described passage and hold the first valve that described movable chamber air enters described inner chamber, described sleeve is provided with air admission hole, is provided with and holds the second valve that outside air enters described movable chamber in described air admission hole.
7. micropipettor as claimed in claim 6, is characterized in that: described air admission hole peripheral hardware has the screen pack for filtered air.
8. the micropipettor as described in any one of claim 1-5, is characterized in that: the upper end of described core pipe is provided with and enters the mouth of pipe for what add capillary in described container cavity, described in enter on the mouth of pipe to be covered with pipe lid.
9. micropipettor as claimed in claim 8, is characterized in that: the upper end of described core pipe is arranged with soft seal cover, and the upper end outer wall of described pipe lid and described shell is supported at described soft seal cover two ends respectively.
10. micropipettor as claimed in claim 1, it is characterized in that: described shell comprises body portion section and head sections, described head sections and described body portion section are tightly connected.
CN201420485623.8U 2014-08-26 2014-08-26 Micropipettor Active CN204051713U (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110672470A (en) * 2018-07-03 2020-01-10 宁德时代新能源科技股份有限公司 Pole piece infiltration testing method and device of secondary battery

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110672470A (en) * 2018-07-03 2020-01-10 宁德时代新能源科技股份有限公司 Pole piece infiltration testing method and device of secondary battery
CN110672470B (en) * 2018-07-03 2021-11-09 宁德时代新能源科技股份有限公司 Pole piece infiltration testing method and device of secondary battery

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C14 Grant of patent or utility model
GR01 Patent grant
PE01 Entry into force of the registration of the contract for pledge of patent right

Denomination of utility model: Portable loader for micropipette tip box

Effective date of registration: 20151223

Granted publication date: 20141231

Pledgee: Bank of China Limited by Share Ltd. Shenzhen East Branch

Pledgor: BGI SHENZHEN Co.,Ltd.

Registration number: 2015990001152

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Pledgor: BGI SHENZHEN Co.,Ltd.

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Denomination of utility model: Portable loader for micropipette tip box

Effective date of registration: 20181029

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Pledgor: BGI SHENZHEN Co.,Ltd.

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