CN201729886U - Novel recovery device for recovering DNA in gel - Google Patents
Novel recovery device for recovering DNA in gel Download PDFInfo
- Publication number
- CN201729886U CN201729886U CN2010201815011U CN201020181501U CN201729886U CN 201729886 U CN201729886 U CN 201729886U CN 2010201815011 U CN2010201815011 U CN 2010201815011U CN 201020181501 U CN201020181501 U CN 201020181501U CN 201729886 U CN201729886 U CN 201729886U
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- CN
- China
- Prior art keywords
- dna
- gel
- pipe
- containing tube
- sample hose
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to a novel recovery device for recovering DNA in gel, which is characterized by comprising a sample pipe (1) and a containing pipe (2), wherein the sample pipe (1) and the containing pipe (2) are manually fastened to form a sealed structure, the sample pipe (1) comprises a pipe cover (3) and a pipe body (4), the pipe cover (3) is provided with a groove (5), the front end of the containing pipe (2) is provided with an opening (6), and the groove (5) and the opening (6) are respectively covered with a dialysis membrane (7). The utility model is simple and practical, has fewer condition requirements, and can effectively recover the DNA in the gel.
Description
Technical field
The utility model relates to the novel retrieving arrangement of recovery DNA in a kind of gel, is suitable for two and reclaims DNA from gel.Belong to medical instruments field.
Background technology
At present, reclaiming DNA from gel mainly is to adopt DNA adsorption column absorption method, and the advantage of this DNA adsorption column absorption method is quick, but has two defectives: the one, and described adsorption column complex structure, cost height; The 2nd, described adsorption column is low to the rate of recovery of large fragment DNA and minimum segment DNA.In addition, also usually adopt certain methods to reclaim DNA in the gel in the laboratory, such as glass bead method, dialysis tubing method etc., there is the low defective of the rate of recovery in these methods equally except the shortcoming that has requirement of experiment height, complex operation.
The utility model content
The purpose of this utility model is to be saved as the problem high, that the DNA rate of recovery is low in order to solve the above-mentioned existing DNA that reclaims from gel, provides in a kind of simple and practical, cost recovery is low, recovering effect is good gel and reclaims the novel retrieving arrangement of DNA.
The purpose of this utility model can reach by the following technical programs:
Reclaim the novel retrieving arrangement of DNA in the gel, its constructional feature is: comprise sample hose 1 and containing tube 2, sample hose 1 and containing tube 2 fasten mutually and form enclosed construction; Described sample hose 1 comprises that pipe cover 3 and body 4, is provided with groove 5 on 3 managing to cover, and is provided with opening 6 at the front end of containing tube 2, is coated with dialysis membrane 7 respectively at described groove 5 and described opening 6 places.
Purpose of the present invention can also reach by the following technical programs:
A kind of embodiment of the present utility model is: described pipe lid 3 is the open-close type elasticity locking connection structure with fixed connection point with body 4, and perhaps described pipe lid 3 is split type elasticity locking connection structure with body 4.
A kind of embodiment of the present utility model is: be provided with internal orifice 8 on described body 4, pass through plastics or resin formation reticulated structure at these internal orifice 8 places.
The present invention has following outstanding beneficial effect:
1, the utility model utilization dna fragmentation principle that anode moves under electric field action (with the dna gel electrophoresis), containing tube is set, the DNA electrophoresis in the gel to the liquid of containing tube, can be reached the purpose that reclaims DNA in the gel, reclaiming DNA does not have the restriction of clip size, rate of recovery height.
2, the utility model is easy and simple to handle, and practicality is not high to conditional request, and the laboratory, the department that possess deposition condition all can use, and can effectively reclaim the DNA in the gel, and good applying value and prospect are arranged.
Description of drawings
Fig. 1 is a structural representation of the present utility model.
Fig. 2 is the pipe lid synoptic diagram of sample hose.
Fig. 3 is the section of structure of containing tube.
Wherein, the 1-sample hose, the 2-containing tube, 3-manages lid, 4-body, 5-groove, 6-opening, 7-dialysis membrane, 8-internal orifice.
Embodiment
Specific embodiment 1:
Fig. 1, Fig. 2 and Fig. 3 constitute specific embodiment of the utility model 1
With reference to Fig. 1, Fig. 2 and Fig. 3, present embodiment comprises sample hose 1 and containing tube 2, and sample hose 1 and containing tube 2 fasten mutually and form enclosed construction; Described sample hose 1 comprises that pipe cover 3 and body 4, is provided with groove 5 on 3 managing to cover, and is provided with opening 6 at the front end of containing tube 2, is coated with dialysis membrane 7 respectively at described groove 5 and described opening 6 places.
In the present embodiment, described pipe lid 3 is the open-close type elasticity locking connection structure with fixed connection point with body 4; On described body 4, be provided with internal orifice 8, pass through plastics or resin formation reticulated structure at these internal orifice 8 places.
The principle of work of present embodiment:
During use, the gel piece that will contain DNA is put into sample hose 1, again sample hose 1 is put into containing tube 2, and be full of electrophoresis liquid, cover the pipe lid 3 of sample hose 1 then, sample hose 1 and containing tube 2 snapped together, with the DNA electrophoresis in the gel to the liquid of containing tube 2; Groove 5 and opening 6 are coated with dialysis membrane 7 and since dialysis membrane 7 be a kind of be the separatory membrane of impellent with the concentration difference, can separate the inside and outside electrophoresis liquid of pipe; The reticulated structure of internal orifice 8 makes gel piece can not enter containing tube 2, can remove gel piece when electrophoresis is finished simultaneously.
Specific embodiment 2:
The characteristics of the utility model specific embodiment 2 are: described pipe lid 3 is split type elasticity locking connection structure with body 4, and internal orifice 8 can be made reticulated structure by resin.All the other are with specific embodiment 1.
Claims (3)
1. reclaim the novel retrieving arrangement of DNA in the gel, it is characterized in that: comprise sample hose (1) and containing tube (2), sample hose (1) and containing tube (2) fasten mutually and form enclosed construction; Described sample hose (1) comprises pipe lid (3) and body (4), is provided with groove (5) on Guan Gai (3), is provided with opening (6) at the front end of containing tube (2), locates to be coated with respectively dialysis membrane (7) at described groove (5) and described opening (6).
2. reclaim the novel retrieving arrangement of DNA in the gel according to claim 1, it is characterized in that: described pipe lid (3) is the open-close type elasticity locking connection structure with fixed connection point with body (4), and perhaps described pipe lid (3) is split type elasticity locking connection structure with body (4).
3. reclaim the novel retrieving arrangement of DNA in the gel according to claim 1, it is characterized in that: on described body (4), be provided with internal orifice (8), locate by plastics or resin formation reticulated structure at this internal orifice (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010201815011U CN201729886U (en) | 2010-04-29 | 2010-04-29 | Novel recovery device for recovering DNA in gel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010201815011U CN201729886U (en) | 2010-04-29 | 2010-04-29 | Novel recovery device for recovering DNA in gel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201729886U true CN201729886U (en) | 2011-02-02 |
Family
ID=43521045
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010201815011U Expired - Fee Related CN201729886U (en) | 2010-04-29 | 2010-04-29 | Novel recovery device for recovering DNA in gel |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201729886U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108572099A (en) * | 2018-04-23 | 2018-09-25 | 上海交通大学 | A kind of method and apparatus for realizing biological tissue's rapid dyeing |
-
2010
- 2010-04-29 CN CN2010201815011U patent/CN201729886U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108572099A (en) * | 2018-04-23 | 2018-09-25 | 上海交通大学 | A kind of method and apparatus for realizing biological tissue's rapid dyeing |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110202 Termination date: 20120429 |