CN2704044Y - Simple filtration dialysis collecting device - Google Patents
Simple filtration dialysis collecting device Download PDFInfo
- Publication number
- CN2704044Y CN2704044Y CN 200320116908 CN200320116908U CN2704044Y CN 2704044 Y CN2704044 Y CN 2704044Y CN 200320116908 CN200320116908 CN 200320116908 CN 200320116908 U CN200320116908 U CN 200320116908U CN 2704044 Y CN2704044 Y CN 2704044Y
- Authority
- CN
- China
- Prior art keywords
- dialysis
- collection tube
- filtration
- application
- sample pipe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Sampling And Sample Adjustment (AREA)
Abstract
The utility model relates to a simple filtration dialysis collecting device used for collecting zone electrophcresis, which is composed of a sample adding pipe, a collecting pipe, a hyperfiltration membrane, a dialyzing membrane, a sealing ring and a perforating. The device overcomes the defect that in the prior art, the cost is high, the structure is complicated, and the filtration and the dialysis can not be simultaneously proceeded; a simple device can be fabricated in a very low cost, and moreover, the filtration and the dialysis can be simultaneously proceeded; the scientific research time and cost are saved. The simple filtration dialysis collecting device can be applied to the biologic experimental research field.
Description
Affiliated technical field
The utility model relates to a kind of simple and easy filtration dialysis gathering-device, and it is the filtration dialysis gathering-device that is used to collect electrophoretic band for a dragon.
Background technology
At present, on the market the known wash-out device that is used to collect electrophoretic band have multiple, as the GE200 type gel elution instrument of Sweden PARMACIA company; The gel elution instrument of U.S. Bio-Rad company; Electroelution instrument/concentrator of U.S. C.B.S; The elution instrument that China Beijing Liu Yichang produces etc., these wash-out apparatus structure complexity, price is comparatively expensive, and after sample filters, also to just can be used for experimental observation, carry out scientific research task for the student working on a postgraduate program of school through dialysis, owing to be subjected to the restriction of experiment fees and experimental period, have a lot of actual difficulties.
Summary of the invention
In order to overcome existing wash-out apparatus structure complexity, price is more expensive, required sample can not filter the problem of dialysis simultaneously, the utility model provides a kind of simple and easy filtration dialysis gathering-device, by the application of sample pipe, be enclosed within application of sample pipe lower end outer wall collection tube, constitute at the ultra filtration membrane of application of sample pipe bottom, dialysis membrane in the collection tube bottom, between application of sample pipe and the collection tube by being tightly connected, it is characterized in that: collection tube upper end tube wall is provided with two parallel perforation, and the upper limb of this perforation is positioned under the bottom of application of sample pipe, and the perforation appearance is stained with insulating tape.
The beneficial effects of the utility model are, the making of this device is simple, and is with low cost, easy and simple to handle, reusable, and yield of specimens is big, and electrophoretic velocity is fast, and filtering dialysis can carry out simultaneously, and collected sample can be directly used in experimental observation.
Description of drawings
The utility model is described in further detail below in conjunction with drawings and Examples.
Fig. 1 is the structural representation of first embodiment of the utility model.
Fig. 2 is the structural representation of second embodiment of the utility model.
According to shown in Figure 1, a kind of simple and easy filter wash is crossed the dialysis gathering-device and is made up of application of sample pipe (1), sealing ring (2), ultra filtration membrane (3), collection tube (4), dialysis membrane (5), perforation (6).Its manufacturing process is as follows: with the bottom that ultra filtration membrane is sealed the bottom of application of sample pipe, sealed collection tube with dialysis membrane, and collection tube upper end and collection tube socket, the blended rubber circle is tightly connected.With the collecting pit horizontal positioned, perforation is slowly injected elution buffer from a side perforation to collection tube with pipettor up earlier; Along with the damping fluid in the collection tube increases gradually, air unnecessary in the collection tube can be discharged from from the opposite side perforation, removes pipettor at last gently, makes and fills with elution buffer in the collecting pit, not residual bubble.Use the insulation adhesive tape with aperture seal subsequently.In the application of sample pipe, inject the ability cathode electrophoresis damping fluid, mounted this wash-out dialysis apparatus is connected in the electrophoresis tank, the dialysis membrane of collection tube bottom is contacted with the anodic electrophoresis buffering liquid, the anode tap and the cathode terminal that guarantee the wash-out dialysis apparatus can form electric field, make charged particle in the sample under the effect of electric field force, pass ultra filtration membrane smoothly, enter in the collection tube, finally concentrate in the collection tube purpose that reaches filtration, dialysis and collect.
As shown in Figure 2, a kind of simple and easy filtration dialysis gathering-device is made up of application of sample pipe (1), sealing ring (2), ultra filtration membrane (3), collection tube (4), dialysis membrane (5), perforation (6), nut pipe (7), outer wall is made screw-like in the lower end of collection tube, the nut pipe that is spirally connected is used for the dialysis membrane of fastening collection tube bottom.
Claims (2)
1. simple and easy filtration dialysis gathering-device, by the application of sample pipe, at the collection tube of application of sample pipe lower end, constitute at the ultra filtration membrane of application of sample pipe bottom, dialysis membrane in the collection tube bottom, between the lower end outer wall of application of sample pipe and the collection tube upper end inwall by being tightly connected, it is characterized in that: collection tube upper end tube wall is provided with two parallel perforation, and the bottom of application of sample pipe is higher than the upper limb of perforation.
2. simple and easy filtration dialysis gathering-device as claimed in claim 1, it is characterized in that: collection tube lower end outer wall surface is screw-like, and overcoat one is used for the nut pipe of fastening dialysis membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200320116908 CN2704044Y (en) | 2003-10-10 | 2003-10-10 | Simple filtration dialysis collecting device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200320116908 CN2704044Y (en) | 2003-10-10 | 2003-10-10 | Simple filtration dialysis collecting device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2704044Y true CN2704044Y (en) | 2005-06-08 |
Family
ID=34762302
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200320116908 Expired - Fee Related CN2704044Y (en) | 2003-10-10 | 2003-10-10 | Simple filtration dialysis collecting device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2704044Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103884570A (en) * | 2014-03-28 | 2014-06-25 | 北京晶泰美康生物科技有限公司 | Integrated electro-eluting and collecting device |
| CN110038434A (en) * | 2019-04-23 | 2019-07-23 | 卢彩婷 | A kind of assemble method of dialysis apparatus and the dialysis apparatus |
-
2003
- 2003-10-10 CN CN 200320116908 patent/CN2704044Y/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103884570A (en) * | 2014-03-28 | 2014-06-25 | 北京晶泰美康生物科技有限公司 | Integrated electro-eluting and collecting device |
| CN110038434A (en) * | 2019-04-23 | 2019-07-23 | 卢彩婷 | A kind of assemble method of dialysis apparatus and the dialysis apparatus |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |